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Sequences

Sequence RAD52.cAug05

AGAGACGCTCGCGCACCCTTCCCATTCTCCTCTGCGCGGCCTCCATCTAAGATCTCTTCCCCTTGTCCATAGCCTAGATCGAGCTCCCTGTGTGCACCGC GCGCTGCCCGAGGCGCCCCAGTGACAGCTGGAGTGGACAAAGTGACCAAGGGCCTCAAGGACACACAAGGCCAAGAAGATTTGAAGTGCTGTAGGTCAAC CAGAATCAAGATGTCTGGGACTGAGGAAGCAATTCTTGGAGGACGTGACAGCCATCCTGCTGCTGGCGGCGGCTCAGTGTTATGCTTTGGACAGTGCCAG TACACAGCAGAAGAGTACCAGGCCATCCAGAAGGCCCTGAGGCAGAGGCTGGGCCCAGAATACATAAGTAGCCGCATGGCTGGCGGAGGCCAGAAGGTGT GCTACATTGAGGGTCATCGGGTAATTAATCTGGCCAATGAGATGTTTGGTTACAATGGCTGGGCACACTCCATCACGCAGCAGAATGTGGATTTTGTTGA CCTCAACAATGGCAAGTTCTACGTGGGAGTCTGTGCATTTGTGAGGGTCCAGCTGAAGGTGAGGGGATGGAGTAGACCTGCTGCCCGGAAGGATCAGTGG GTGGTAGGAGAAGGATGGTTCATATCATGAAGATGTTGGTTATGGTGTTAGTGAGGGCCTCAAGTCCAAGGCTTTATCTTTGGAGAAGGCAAGGAAGGAG GCGGTGACAGACGGGCTGAAGCGAGCCCTCAGGCTTCCCTTGCTGGGAGTAAGTGGTAGAATCCTGTACTCTCTCTTCTCCGTGCACTCAGTCATGTGTG CCGGAGGCCTTCCCACTCCTACTGCGAGTGCACAGACAGCACCCTCCTCTCCGTGCTCCTCAGCAGTCCTCCGCTACGCACAGGAGTTTTGGGAATGCAC TTGGAAACTGTATTCTGGACAAAGACTACCTGAGATCACTAAATAAGCTTCCACGCCAGTTGCCTCTTGAAGTGGATTTAACTAAAGCGAAGAGACAAGA TCTTGAACCGTCTGTGGAGGAGGCAAGATACAACAGCTGCCGACCGAACATGGCCCTGGGACACCCACAGCTGCAGCAGGTGACCTCCCCTTCCAGACCC AGCCATGCTGTGATACCGGCGGACCAGGACTGCAGCTCCCGAAGCCTGAGCTCATCCGCCGTGGAGAGCGAGGCCACGCACCAGCGGAAGCTCCGGCAGA AGCAGCTGCAGCAGCAGTTCCGGGAGCGGATGGAGAAGCAGCAGGTTCGAGTCTCCACGCCGTCAGCTGAGAAGAGTGAGGCAGCGCCTCCGGCCCCTCC TGTGACGCACAGCACTCCTGTAACTGTCTCAGAACCACTCCTGGAGAAAGACTTCCTTGCAGGAGTGACTCAAGAATTAATCAAGACTCTTGAAGACAAC TCTGAAAAGTGGGCTGTGACTCCCGATGCAGGGGATGGTGTGGTCAAGCCCTCGTCTAGAGCAGACCCAGCCCAGACCTCTGACACATTAGCCTTGAACA ACCAGATGGTGACCCAGAACAGGACTCCACACAGCGTTTGCCACCAGAAACCACAAGCAAAATCTGGATCTTGGGACCTCCAAACTTATAGCGCTGACCA ACGCACAACAGGAAACTGGGAATCTCATAGGAAGAGCCAGGACATGAAGAAAAGGAAATATGATCCATCTTAACTGAGGCTCAGGCCACATAATTGGACT CTGTCACAAAGGGACTTTGGAAAACTACTTTTTGGTCATGAAATTGTTCATCGCTGCTGGAGAATGAACGTCATTGCGATTTATCTTGCTTCATTCTGAA CCTTATCAAGAGGATCTGACTGAGAGCCCACTGCAGTTAGAGCTGAGCACTTTTGAAAAGCTTGTCCATCACTCTAGTAGGGAGAGGCTCTGGACAGATG AATACCTTTTCTTCGGCTTGTGAGGCTTCCCACTATTTATTACTGAACTATTATGTTAATGAAGATGGACATTTTAGGAATCACCAATGGCTCCTTGCCC TCAAGCAATATAGGCCAGACTTGGTCCTAAGCACCTGCCTCAGCAATTGTCTACATTCAGTTGTTTTGCATAACGTCTGCCTTCTTTCCTTTACGGTCCA TGCCTTTAATGTTGCCCACATTAAGCACTGTGGATCACGACAGGAAAAAGGTTGGAGCAGTGCTTTTCACTACTTTGTATCAATCCAGGCTACAATCTTC ATTTAATATAAATAATTTATGGATTTATGACATTACAATCCTGCATTGTTTCAAGACTGACATTTTTTCCTAAGGAAGGAAATAATCATCTAAGACCACG AAAAAAGGCTGTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTGAGACGGGGTCTGGCTGTGTTGCCCTGACTGGAGTTCAGTGGTGCAAACACAGCTCTCT CCACAACCTCTTGGGCCCAAGTGATACTCCCACCTCTGCCTTACAAAATACAGGGATTACTGGTGTGAGCCACTGTGTCTGGCCAGAAAAGGCATTTTTG AGAAAGCAAATCGTATACCTTATTAACAAAATAGAATATATATATATTGCTTATCTGAAATGCTTGAAACCAGAATTGTTTTGCATTTTTTGAATATTTG TATACACATAATGAGACCTTGGGGATGGGACCCAAGTCTGAACGTGGAATTCACCTGTGTTTCGTGTATATGCCTCATACACATAATTTTGTGCATGAAA CAGAGTTTTTGTATAAGAAGATACACTGCAGCTGAAGAGGGCTGGGTTTTTTTTTCTCTTAGGGTCGCTGCATAAACTGTTGTATGCCTGGTGCTTTGCG ACTTGTCACACGAGGTCACGTGTGGAATTTTCCACTTCTGGCATCACGTCAGTGCTCAGAAATTTTCTGATCTCAGAGCATTTCAATTAGGGATGCTCAA ACGCAACTGTTTCTACTTCCCCATTTCAGGTGTGAGATGTAACCCACCTTGACCATAAATTGGCTTTTCATAGTGCTCAGATGTTTCATAATTCTTCCCC CAAAATAATGAAGTTTGCAGTTTAACAACTTTTTTTTTGAGAGGGGGGGTCTGGCTCTGTAGCGCAGGCTGGAGTGCAGCGGTGCCATCTTGACTCATTG CAGCCTCCGCCTGCCAGGATTCAAGCAATTCTCCTGCCTCAGGCTCCCGAGTAGCTGGGATTACAGACACCCACCACGACGCCCGACTAATTTTTGTATT TTTAGTAGAGGAGACGGGGTTTCTCCATGTTGGCCAGGCTGGTCTCAAACTGACCTCAAGTGATCTGCCCACCTCGGCCTCCCAAAGTAAAAAGTACTAG AATTATAGGCATGAGCCATTGCGCCCAGCCAATTTAATAATTTTTTTAGACGTATTATTTTGGGCTGGGCTGTGGCTAATCTTGAAGCAGTGACTTCCTA TTTTTTGGCTCAGACCCATTTGGGAAGCTGTGAACCTGAGATAAGGAAGTCTGAAAGTACTAGCCGGCAGCCGACCGTTTCTATTCCTTTCGAAGTTAAG GAGACAGCAAAGGAGCTCCCAGGAATTAGGGATCCAGTTATAAATTGCACTTAACTCAAAATAGTGCCAGTGAGAATGTGAAGGAATCTTCTGGAAATGA AACCCCACAGCTCAACAAAAGCAAGTTTGGTTTATTTGAATGGTTTCATTAAATAAGTCTACAAAGGTAACAAACTGAAGCACAAAGCGCAATCTTACAA GAAGCGCAACACCCAGAGTTAGTGCAAAATGTACAATTAACGGCTGCTGAGAAACCCCCAAAGTTTGAATTTTTATTTTTATTTTTAACAGTGTACATTG TTAAATAATGTAAGGAAAACATTTATAATTCAGAAAGTTTTTAATGTGGAAAAAGATACTCAAAGTGTACTATTAACACAAAAATAATTTAAACAGTTCA GTAGCACTAGTTCATTCCTCTAGGCTTGTTATTTCATACACAGGAGGATGAAATCAACAGTAAATAACACCAGTTCTGACTTTTTTTGTGTATGAGTGAA AAGATGTCAAAATTATTATTAAACCCAAATAACAAATGATAATGTGCTGCATGAGGCTCCACTGCTCCTCTGGTATAAAGTATGTTTCACTCTTGGGTGG CCCCTTTCTCAGCTACTCAGCCCCTTGTTGGTTCTATCCTTACTCTCTCCACAAGAAATACAAGCAGAGAATAAATAAATCATTTTAAAGCAGGGGTGGA AGCCCTTGCCCTTTTCCTTTCTACCTTTTAAAAACCTTCAGCTCTCATTTGCATATTTAGGGCCGTAGACCAACTTACTTACTTACTAAGGGAAGCAGGG CCCTTAAGGAATGAACAGGGTACAGGAGCCTCCAGCCTCTTTCTGTGGTAGTGGTGGTGGTGGTGGTGATTCCCAGAATATGCCATTGACGTGGGTAAAA GGTTGGAAATATTTTCAGTTTCCCAACTAAGAGTCAAGAAGCACCCCAAAATTAAGAGTCCTAGCTTCTTTCTTTGTCTTCAGAGATAACAAAGATCCTA AACATAGCCAGTTAGGACAGTATTTCCGGAAAGCTCTGAAAATAATTAATAACTACCCATTACAAGGAGGCATAGCACTGAATAACACTGATTGGTTGCA GTTATTACTTACGGGGAGTATTATTTATTTGAAAATGTGAACATATTAGTTGTACTTCTAGAAATAAAGGTGGCACAATAGATTATGATCCTGCAACCAT TTGGACATCATAAAGGAAATGAGTAGGAATAAGTATTCTTTCCTAGAATGATTAATTCAGATGTGGAAAGATAGATAGATTAACTTTCCACAAAATGTAG TACCCCAAGCTGAGAAATGCATGGTACATGTGCAACAAGAGGAAAGCAATCCAAACAGTTTTTCTGAGAGCAATTTTCTCCATCTGTGACTTTTCACTAT CAGGTTCCCCAACTTGAAAACTAGCCCCCCACCCCCTTCTCTTTATGTTCAGTTTCTTCTAGGTCACGTCTGTTCAAGCCTAGCTCAAATAAGAAAGGTT TATATGACAGTCACTGGCCAGGCATCACGTTTGTTTCCTCATTCTTTAGTCTTTGTCTTTCACCCAGCACACACCATTCTTTTAAGTTGTCCAATTTTGT TACAACACCCCCTCCTCACTATTCCCGAAACAGAACTTTCTGTTTTTAACCACTGATGCCAACCTAACTTCCCACCCTACTCATCTTACACCTGCTCTAG ACAAAGTTGTTGAATGACTTAAAAAAATACTTTTTCAATTTCCCTTTGGCATAACCACTGCTGGACTGCTGGGGTCACATGGACCTACGATTTCCTTCCA CATGCCCTTTATTGATCTGGGGTCTGGTAAGTTGATGCTAATGGCAGAAGCAATAGAAGTCCAAGGCACTAAAATGTCCAGCAAGCAACACTGGGAAACA GAAGTATTCAAGTACAGTAATTTATACAATTTTACATTCACATTTATTTATCTAATACAATGGAAATACAAAGGAAAAAGTTAAAGTGTCTCAATAAGTA AAGGACAGAGCTAGCTTTTTTTGGAACTTTTTAAACGTTTCATATTTTACTCTTAAAACTACTGTTGTCCAACAACATTTTATATATCACAGTGTTTCCA CATCAAAACTGATGGCAAAGTTACATGTCCGCAGGGAACTTAATTTTTTTTTTTTCCTAATTGCTAATGCTGCAGTCAAAGCTGCTAATATCTTTTGCTT AAAGCACTAATGACCTATCATAAGAAAGAGTTGCACCCCAGTACCCTGGTATTTTTCACTGGGTTCTAAAGAGCAAAGAAAAAATAAAAAAGAGAGGGGT AAAAAAAAGGGAGGAGGGCAGGGTAGGAGAGAGCCCAGTAGGTGGTTTTTGTCCTGTGGTTCGGATTCTTGAGGGAGTGAGGGATTCAGGGCATGTACCA AAAGCAAGAGCTCCACCCACTCGAAATTACAGATGGGTTTGATTTTACATTGAGTTCTCCAGCTTCCTTATGAACAAGACTTTCCTAGCCCTCATCCTCA TTACTGAGTATAGATAAACAGGAAAGCATGGTCTGAAGCTGCCCCTTGACAAAAAAGCTGTTCTTTCCTTCCTCTCCCATTCCAATAACTGGAGCGGGGG AGACTCTCCATTCTGAGGGCTCAATGCAGTATTAAAAACATTCCCCTCTGGCAAGAAATAACCAGTTAAATGCAGCACTAGTAGTTAGTATATAGGCTAC TTCCCACCCCCACCCACCCCCTCAGCATTCCATCTCCTGCCCCCAGATCTATTCAGTTAATGTCTCTAGGTCTAAGTGGTCCGCAGGTTGGAGCCTGGGG GGTTGCTGATGGATTTCTGCAAATTGCTGATGTTGAAATTCTGCAAGGAGGCAGTTCCCACAGGTTGGAACTGGCCAAGTGGCTGTGGTGCTGGGCCACC CGTCCCACTCCATTGAGCGCCAAATGGGGTAGCTGGAAAGCCATACTGCTGTGGGGGGCACATAGACTTGGTGAAGTGACCTAGAGAGGTAGCTGATGCT GCAGAGATGGGGGCAGAGCCACCCAGGTTCGAGGTCATGGAGATGCACAGTTGCCCAGGTGCAGAGAACTTCCTTGCCATTCCAGGGCCCTGAAAACAAA ACCACCACGTGTCAAGTCTGGTGTGGGCCATGGTATAAAATGTGTGGCGCTAACATATACTTCTAGTATAATCCTGTAAGATACAGCAGAACAGTCTTCT GTAGTATTTATTGGCTCTTTATATTTACCAAGTAAGGGTGAAAATAATCCTCATGAGAGGGAGGGATGTCATGTGCGTGTGTGTCTGTGTGTGTGTAGTT GAGGATGACGAAGG

RAD52.u.f.18 (forward primer)
RAD52.u.r.18 (reverse primer)
Intron retention

Frame 0

RDARAPFPFSSARPPSKISSPCP*PRSSSLCAP RAARGAPVTAGVDKVTKGLKDTQGQEDLKCCRST RIKMSGTEEAILGGRDSHPAAGGGSVLCFGQCQ YTAEEYQAIQKALRQRLGPEYISSRMAGGGQKV CYIEGHRVINLANEMFGYNGWAHSITQQNVDFVD LNNGKFYVGVCAFVRVQLKVRGWSRPAARKDQW VVGEGWFIS*RCWLWC**GPQVQGFIFGEGKEG GGDRRAEASPQASLAGSKW*NPVLSLLRALSHVC RRPSHSYCECTDSTLLSVLLSSPPLRTGVLGMH LETVFWTKTT*DH*ISFHASCLLKWI*LKRRDK ILNRLWRRQDTTAADRTWPWDTHSCSR*PPLPDP AML*YRRTRTAAPEA*AHPPWRARPRTSGSSGR SSCSSSSGSGWRSSRFESPRRQLRRVRQRLRPL L*RTALL*LSQNHSWRKTSLQE*LKN*SRLLKTT LKSGL*LPMQGMVWSSPRLEQTQPRPLTH*P*T TRW*PRTGLHTAFATRNHKQNLDLGTSKLIALT NAQQETGNLIGRART*RKGNMIHLN*GSGHIIGL CHKGTLENYFLVMKLFIAAGE*TSLRFILLHSE PYQEDLTESPLQLELSTFEKLVHHSSRERLWTD EYLFFGL*GFPLFITELLC**RWTF*ESPMAPCP QAI*ARLGPKHLPQQLSTFSCFA*RLPSFLYGP CL*CCPH*ALWITTGKRLEQCFSLLCINPGYNL HLI*IIYGFMTLQSCIVSRLTFFPKEGNNHLRPR KKAVFCFFFFFFF*DGVWLCCPDWSSVVQTQLS PQPLGPK*YSHLCLTKYRDYWCEPLCLARKGIF EKANRIPY*QNRIYIYCLSEMLETRIVLHFLNIC IHIMRPWGWDPSLNVEFTCVSCICLIHIILCMK QSFCIRRYTAAEEGWVFFSLRVAA*TVVCLVLC DLSHEVTCGIFHFWHHVSAQKFSDLRAFQLGMLK RNCFYFPISGVRCNPP*P*IGFS*CSDVS*FFP QNNEVCSLTTFFLRGGVWLCSAGWSAAVPS*LI AASACQDSSNSPASGSRVAGITDTHHDARLIFVF LVEETGFLHVGQAGLKLTSSDLPTSASQSKKY* NYRHEPLRPANLIIFLDVLFWAGLWLILKQ*LP IFWLRPIWEAVNLR*GSLKVLAGSRPFLFLSKLR RQQRSSQELGIQL*IALNSK*CQ*ECEGIFWK* NPTAQQKQVWFI*MVSLNKSTKVTN*STKRNLT RSATPRVSAKCTINGC*ETPKV*IFIFIFNSVHC *IM*GKHL*FRKFLMWKKILKVYY*HKNNLNSS VALVHSSRLVISYTGG*NQQ*ITPVLTFFVYE* KDVKIIIKPK*QMIMCCMRLHCSSGIKYVSLLGG PFLSYSAPCWFYPYSLHKKYKQRINKSF*SRGG SPCPFPFYLLKTFSSHLHI*GRRPTYLLTKGSR ALKE*TGYRSLQPLSVVVVVVVVIPRICH*RG*K VGNIFSFPTKSQEAPQN*ES*LLSLSSEITKIL NIAS*DSISGKL*K*LITTHYKEA*H*ITLIGC SYYLRGVLFI*KCEHISCTSRNKGGTIDYDPATI WTS*RK*VGISILS*ND*FRCGKIDRLTFHKM* YPKLRNAWYMCNKRKAIQTVFLRAIFSICDFSL SGSPT*KLAPHPLLFMFSFF*VTSVQA*LK*ERF I*QSLARHHVCFLIL*SLSFTQHTPFF*VVQFC YNTPSSLFPKQNFLFLTTDANLTSHPTHLTPAL DKVVE*LKKILFQFPFGITTAGLLGSHGPTISFH MPFIDLGSGKLMLMAEAIEVQGTKMSSKQHWET EVFKYSNLYNFTFTFIYLIQWKYKGKS*SVSIS KGQS*LFLELFKRFIFYS*NYCCPTTFYISQCFH IKTDGKVTCPQGT*FFFFPNC*CCSQSC*YLLL KALMTYHKKELHPSTLVFFTGF*RAKKK*KREG *KKGRRAG*ERAQ*VVFVLWFGFLRE*GIQGMYQ KQELHPLEITDGFDFTLSSPASL*TRLS*PSSS LLSIDKQESMV*SCPLTKKLFFPSSPIPITGAG ETLHSEGSMQY*KHSPLARNNQLNAALVVSI*AT SHPHPPPQHSISCPQIYSVNVSRSKWSAGWSLG GC*WISANC*C*NSARRQFPQVGTGQVAVVLGH PSHSIERQMG*LESHTAVGGT*TW*SDLER*LML QRWGQSHPGSRSWRCTVAQVQRTSLPFQGPENK TTTCQVWCGPWYKMCGANIYF*YNPVRYSRTVF CSIYWLFIFTK*G*K*SS*EGGMSCACVSVCV*L RMTK

Frame 1

ETLAHPSHSPLRGLHLRSLPLVHSLDRAPCVHR ALPEAPQ*QLEWTK*PRASRTHKAKKI*SAVGQ PESRCLGLRKQFLEDVTAILLLAAAQCYALDSAS TQQKSTRPSRRP*GRGWAQNT*VAAWLAEARRC ATLRVIG*LIWPMRCLVTMAGHTPSRSRMWILL TSTMASSTWESVHL*GSS*R*GDGVDLLPGRISG W*EKDGSYHEDVGYGVSEGLKSKALSLEKARKE AVTDGLKRALRLPLLGVSGRILYSLFSVHSVMC AGGLPTPTASAQTAPSSPCSSAVLRYAQEFWECT WKLYSGQRLPEITK*ASTPVAS*SGFN*SEETR S*TVCGGGKIQQLPTEHGPGTPTAAAGDLPFQT QPCCDTGGPGLQLPKPELIRRGERGHAPAEAPAE AAAAAVPGADGEAAGSSLHAVS*EE*GSASGPS CDAQHSCNCLRTTPGERLPCRSDSRINQDS*RQ L*KVGCDSRCRGWCGQALV*SRPSPDL*HISLEQ PDGDPEQDSTQRLPPETTSKIWILGPPNL*R*P THNRKLGIS*EEPGHEEKEI*SILTEAQAT*LD SVTKGLWKTTFWS*NCSSLLENERHCDLSCFILN LIKRI*LRAHCS*S*ALLKSLSITLVGRGSGQM NTFSSACEASHYLLLNYYVNEDGHFRNHQWLLA LKQYRPDLVLSTCLSNCLHSVVLHNVCLLSFTVH AFNVAHIKHCGSRQEKGWSSAFHYFVSIQATIF I*YK*FMDL*HYNPALFQD*HFFLRKEIII*DH EKRLFFVFFFFFFFETGSGCVALTGVQWCKHSSL HNLLGPSDTPTSALQNTGITGVSHCVWPEKAFL RKQIVYLINKIEYIYIAYLKCLKPELFCIF*IF VYT**DLGDGTQV*TWNSPVFRVYASYT*FCA*N RVFV*EDTLQLKRAGFFFLLGSLHKLLYAWCFA TCHTRSRVEFSTSGITSVLRNFLISEHFN*GCS NATVSTSPFQV*DVTHLDHKLAFHSAQMFHNSSP KIMKFAV*QLFF*EGGSGSVAQAGVQRCHLDSL QPPPARIQAILLPQAPE*LGLQTPTTTPD*FLY F**RRRGFSMLARLVSN*PQVICPPRPPKVKSTR IIGMSHCAQPI**FF*TYYFGLGCG*S*SSDFL FFGSDPFGKL*T*DKEV*KY*PAADRFYSFRS* GDSKGAPRN*GSSYKLHLTQNSASENVKESSGNE TPQLNKSKFGLFEWFH*ISLQR*QTEAQSAILQ EAQHPELVQNVQLTAAEKPPKFEFLFLFLTVYI VK*CKENIYNSESF*CGKRYSKCTINTKII*TVQ *H*FIPLGLLFHTQEDEINSK*HQF*LFLCMSE KMSKLLLNPNNK**CAA*GSTAPLV*SMFHSWV APFSATQPLVGSILTLSTRNTSRE*INHFKAGVE ALALFLSTF*KPSALICIFRAVDQLTYLLREAG PLRNEQGTGASSLFLW*WWWWW*FPEYAIDVGK RLEIFSVSQLRVKKHPKIKSPSFFLCLQR*QRS* T*PVRTVFPESSENN**LPITRRHSTE*H*LVA VITYGEYYLFENVNILVVLLEIKVAQ*IMILQP FGHHKGNE*E*VFFPRMINSDVER*ID*LSTKCS TPS*EMHGTCATRGKQSKQFF*EQFSPSVTFHY QVPQLEN*PPTPFSLCSVSSRSRLFKPSSNKKG LYDSHWPGITFVSSFFSLCLSPSTHHSFKLSNFV TTPPPHYSRNRTFCF*PLMPT*LPTLLILHLL* TKLLNDLKKYFFNFPLA*PLLDCWGHMDLRFPS TCPLLIWGLVS*C*WQKQ*KSKALKCPASNTGKQ KYSSTVIYTILHSHLFI*YNGNTKEKVKVSQ*V KDRASFFWNFLNVSYFTLKTTVVQQHFIYHSVS TSKLMAKLHVRRELNFFFFLIANAAVKAANIFCL KH**PIIRKSCTPVPWYFSLGSKEQRKNKKERG KKKGGGQGRREPSRWFLSCGSDS*GSEGFRACT KSKSSTHSKLQMGLILH*VLQLPYEQDFPSPHPH Y*V*INRKAWSEAAP*QKSCSFLPLPFQ*LERG RLSILRAQCSIKNIPLWQEITS*MQH**LVYRL LPTPTHPLSIPSPAPRSIQLMSLGLSGPQVGAWG VADGFLQIADVEILQGGSSHRLELAKWLWCWAT RPTPLSAKWGSWKAILLWGAHRLGEVT*RGS*C CRDGGRATQVRGHGDAQLPRCRELPCHSRALKTK PPRVKSGVGHGIKCVALTYTSSIIL*DTAEQSS VVFIGSLYLPSKGENNPHEREGCHVRVCLCVCS *G*RR

Frame 2

RRSRTLPILLCAASI*DLFPLSIA*IELPVCTA RCPRRPSDSWSGQSDQGPQGHTRPRRFEVL*VN QNQDVWD*GSNSWRT*QPSCCWRRLSVMLWTVP VHSRRVPGHPEGPEAEAGPRIHK*PHGWRRPEGV LH*GSSGN*SGQ*DVWLQWLGTLHHAAECGFC* PQQWQVLRGSLCICEGPAEGEGME*TCCPEGSV GGRRRMVHIMKMLVMVLVRASSPRLYLWRRQGRR R*QTG*SEPSGFPCWE*VVESCTLSSPCTQSCV PEAFPLLLRVHRQHPPLRAPQQSSATHRSFGNA LGNCILDKDYLRSLNKLPRQLPLEVDLTKAKRQD LEPSVEEARYNSCRPNMALGHPQLQQVTSPSRP SHAVIPADQDCSSRSLSSSAVESEATHQRKLRQ KQLQQQFRERMEKQQVRVSTPSAEKSEAAPPAPP VTHSTPVTVSEPLLEKDFLAGVTQELIKTLEDN SEKWAVTPDAGDGVVKPSSRADPAQTSDTLALN NQMVTQNRTPHSVCHQKPQAKSGSWDLQTYSADQ RTTGNWESHRKSQDMKKRKYDPS*LRLRPHNWT LSQRDFGKLLFGHEIVHRCWRMNVIAIYLASF* TLSRGSD*EPTAVRAEHF*KACPSL**GEALDR* IPFLRLVRLPTIYY*TIMLMKMDILGITNGSLP SSNIGQTWS*APASAIVYIQLFCITSAFFPLRS MPLMLPTLSTVDHDRKKVGAVLFTTLYQSRLQSS FNINNLWIYDITILHCFKTDIFS*GRK*SSKTT KKGCFLFFFFFFFLRRGLAVLP*LEFSGANTAL STTSWAQVILPPLPYKIQGLLV*ATVSGQKRHF* ESKSYTLLTK*NIYILLI*NA*NQNCFAFFEYL YTHNETLGMGPKSERGIHLCFVYMPHTHNFVHE TEFLYKKIHCS*RGLGFFFS*GRCINCCMPGALR LVTRGHVWNFPLLASRQCSEIF*SQSISIRDAQ TQLFLLPHFRCEM*PTLTINWLFIVLRCFIILP PK**SLQFNNFFFERGGLAL*RRLECSGAILTHC SLRLPGFKQFSCLRLPSSWDYRHPPRRPTNFCI FSRGDGVSPCWPGWSQTDLK*SAHLGLPK*KVL EL*A*AIAPSQFNNFFRRIILGWAVANLEAVTSY FLAQTHLGSCEPEIRKSESTSRQPTVSIPFEVK ETAKELPGIRDPVINCT*LKIVPVRM*RNLLEM KPHSSTKASLVYLNGFIK*VYKGNKLKHKAQSYK KRNTQS*CKMYN*RLLRNPQSLNFYFYF*QCTL LNNVRKTFIIQKVFNVEKDTQSVLLTQK*FKQF SSTSSFL*ACYFIHRRMKSTVNNTSSDFFCV*VK RCQNYY*TQITNDNVLHEAPLLLWYKVCFTLGW PLSQLLSPLLVLSLLSPQEIQAENK*IILKQGW KPLPFSFLPFKNLQLSFAYLGP*TNLLTY*GKQG P*GMNRVQEPPASFCGSGGGGGDSQNMPLTWVK GWKYFQFPN*ESRSTPKLRVLASFFVFRDNKDP KHSQLGQYFRKALKIINNYPLQGGIALNNTDWLQ LLLTGSIIYLKM*TY*LYF*K*RWHNRL*SCNH LDIIKEMSRNKYSFLE*LIQMWKDR*INFPQNV VPQAEKCMVHVQQEESNPNSFSESNFLHL*LFTI RFPNLKTSPPPPSLYVQFLLGHVCSSLAQIRKV YMTVTGQASRLFPHSLVFVFHPAHTILLSCPIL LQHPLLTIPETELSVFNH*CQPNFPPYSSYTCSR QSC*MT*KNTFSISLWHNHCWTAGVTWTYDFLP HALY*SGVW*VDANGRSNRSPRH*NVQQATLGN RSIQVQ*FIQFYIHIYLSNTMEIQRKKLKCLNK* RTELAFFGTF*TFHILLLKLLLSNNILYITVFP HQN*WQSYMSAGNLIFFFS*LLMLQSKLLISFA *STNDLS*ERVAPQYPGIFHWVLKSKEKIKKRGV KKREEGRVGESPVGGFCPVVRILEGVRDSGHVP KARAPPTRNYRWV*FYIEFSSFLMNKTFLALIL ITEYR*TGKHGLKLPLDKKAVLSFLSHSNNWSGG DSPF*GLNAVLKTFPSGKK*PVKCSTSS*YIGY FPPPPTPSAFHLLPPDLFS*CL*V*VVRRLEPG GLLMDFCKLLMLKFCKEAVPTGWNWPSGCGAGPP VPLH*APNGVAGKPYCCGGHIDLVK*PREVADA AEMGAEPPRFEVMEMHSCPGAENFLAIPGP*KQ NHHVSSLVWAMV*NVWR*HILLV*SCKIQQNSLL *YLLALYIYQVRVKIILMRGRDVMCVCVCVCVV EDDE

Sequence RAD52.fAug05

AGAGACGCTCGCGCACCCTTCCCATTCTCCTCTGCGCGGCCTCCATCTAAGATCTCTTCCCCTTGTCCATAGCCTAGATCGAGCTCCCTGTGTGCACCGC GCGCTGCCCGAGGCGCAGGTCAACCAGAATCAAGATGTCTGGGACTGAGGAAGCAATTCTTGGAGGACGTGACAGCCATCCTGCTGCTGGCGGCGGCTCA GTGTTATGCTTTGGACAGTGCCAGTACACAGCAGAAGAGTACCAGGCCATCCAGAAGGCCCTGAGGCAGAGGCTGGGCCCAGAATACATAAGTAGCCGCA TGGCTGGCGGAGGCCAGAAGGTGTGCTACATTGAGGGTCATCGGGTAATTAATCTGGCCAATGAGATGTTTGGTTACAATGGCTGGGCACACTCCATCAC GCAGCAGAATGTGGATTTTGTTGACCTCAACAATGGCAAGTTCTACGTGGGAGTCTGTGCATTTGTGAGGGTCCAGCTGAAGGATGGTTCATATCATGAA GATGTTGGTTATGGTGTTAGTGAGGGCCTCAAGTCCAAGGCTTTATCTTTGGAGAAGGCAAGGAAGGAGGCGGTGACAGACGGGCTGAAGCGAGCCCTCA GGCTTCCCTTGCTGGGAGTAAGTGGTAGAATCCTGTACTCTCTCTTCTCCGTGCACTCAGTCATGTGTGCCGGAGGCCTTCCCACTCCTACTGCGAGTGC ACAGACAGCACCCTCCTCTCCGTGCTCCTCAGCAGTCCTCCGCTACGCACAGGAGTTTTGGGAATGCACTTGGAAACTGTATTCTGGACAAAGACTACCT GAGATCACTAAATAAGCTTCCACGCCAGTTGCCTCTTGAAGTGGATTTAACTAAAGCGAAGAGACAAGATCTTGAACCGTCTGTGGAGGAGGCAAGATAC AACAGCTGCCGACCGAACATGGCCCTGGGACACCCACAGCTGCAGCAGGTGACCTCCCCTTCCAGACCCAGCCATGCTGTGATACCGGCGGACCAGGACT GCAGCTCCCGAAGCCTGAGCTCATCCGCCGTGGAGAGCGAGGCCACGCACCAGCGGAAGCTCCGGCAGAAGCAGCTGCAGCAGCAGTTCCGGGAGCGGAT GGAGAAGCAGCAGGTTCGAGTCTCCACGCCGTCAGCTGAGAAGAGTGAGGCAGCGCCTCCGGCCCCTCCTGTGACGCACAGCACTCCTGTAACTGTCTCA GAACCACTCCTGGAGAAAGACTTCCTTGCAGGAGTGACTCAAGAATTAATCAGTAAATACCTTGAAATGCTTAGACTCTTGAAGACAACTCTGAAAAGTG GGCTGTGACTCCCGATGCAGGGGATGGTGTGGTCAAGCCCTCGTCTAGAGCAGACCCAGCCCAGACCTCTGACACATTAGCCTTGAACAACCAGATGGTG ACCCAGAACAGGACTCCACACAGCGTTTGCCACCAGAAACCACAAGCAAAATCTGGATCTTGGGACCTCCAAACTTATAGCGCTGACCAACGCACAACAG GAAACTGGGAATCTCATAGGAAGAGCCAGGACATGAAGAAAAGGAAATATGATCCATCTTAACTGAGGCTCAGGCCACATAATTGGACTCTGTCACAAAG GGACTTTGGAAAACTACTTTTTGGTCATGAAATTGTTCATCGCTGCTGGAGAATGAACGTCATTGCGATTTATCTTGCTTCATTCTGAACCTTATCAAGA GGATCTGACTGAGAGCCCACTGCAGTTAGAGCTGAGCACTTTTGAAAAGCTTGTCCATCACTCTAGTAGGGAGAGGCTCTGGACAGATGAATACCTTTTC TTCGGCTTGTGAGGCTTCCCACTATTTATTACTGAACTATTATGTTAATGAAGATGGACATTTTAGGAATCACCAATGGCTCCTTGCCCTCAAGCAATAT AGGCCAGACTTGGTCCTAAGCACCTGCCTCAGCAATTGTCTACATTCAGTTGTTTTGCATAACGTCTGCCTTCTTTCCTTTACGGTCCATGCCTTTAATG TTGCCCACATTAAGCACTGTGGATCACGACAGGAAAAAGGTTGGAGCAGTGCTTTTCACTACTTTGTATCAATCCAGGCTACAATCTTCATTTAATATAA ATAATTTATGGATTTATGACATTACAATCCTGCATTGTTTCAAGACTGACATTTTTTCCTAAGGAAGGAAATAATCATCTAAGACCACGAAAAAAGGCTG TTTTTTGTTTTTTTTTTTTTTTTTTTTTTTGAGACGGGGTCTGGCTGTGTTGCCCTGACTGGAGTTCAGTGGTGCAAACACAGCTCTCTCCACAACCTCT TGGGCCCAAGTGATACTCCCACCTCTGCCTTACAAAATACAGGGATTACTGGTGTGAGCCACTGTGTCTGGCCAGAAAAGGCATTTTTGAGAAAGCAAAT CGTATACCTTATTAACAAAATAGAATATATATATATTGCTTATCTGAAATGCTTGAAACCAGAATTGTTTTGCATTTTTTGAATATTTGTATACACATAA TGAGACCTTGGGGATGGGACCCAAGTCTGAACGTGGAATTCACCTGTGTTTCGTGTATATGCCTCATACACATAATTTTGTGCATGAAACAGAGTTTTTG TATAAGAAGATACACTGCAGCTGAAGAGGGCTGGGTTTTTTTTTCTCTTAGGGTCGCTGCATAAACTGTTGTATGCCTGGTGCTTTGCGACTTGTCACAC GAGGTCACGTGTGGAATTTTCCACTTCTGGCATCACGTCAGTGCTCAGAAATTTTCTGATCTCAGAGCATTTCAATTAGGGATGCTCAAACGCAACTGTT TCTACTTCCCCATTTCAGGTGTGAGATGTAACCCACCTTGACCATAAATTGGCTTTTCATAGTGCTCAGATGTTTCATAATTCTTCCCCCAAAATAATGA AGTTTGCAGTTTAACAACTTTTTTTTTGAGAGGGGGGGTCTGGCTCTGTAGCGCAGGCTGGAGTGCAGCGGTGCCATCTTGACTCATTGCAGCCTCCGCC TGCCAGGATTCAAGCAATTCTCCTGCCTCAGGCTCCCGAGTAGCTGGGATTACAGACACCCACCACGACGCCCGACTAATTTTTGTATTTTTAGTAGAGG AGACGGGGTTTCTCCATGTTGGCCAGGCTGGTCTCAAACTGACCTCAAGTGATCTGCCCACCTCGGCCTCCCAAAGTAAAAAGTACTAGAATTATAGGCA TGAGCCATTGCGCCCAGCCAATTTAATAATTTTTTTAGACGTATTATTTTGGGCTGGGCTGTGGCTAATCTTGAAGCAGTGACTTCCTATTTTTTGGCTC AGACCCATTTGGGAAGCTGTGAACCTGAGATAAGGAAGTCTGAAAGTACTAGCCGGCAGCCGACCGTTTCTATTCCTTTCGAAGTTAAGGAGACAGCAAA GGAGCTCCCAGGAATTAGGGATCCAGTTATAAATTGCACTTAACTCAAAATAGTGCCAGTGAGAATGTGAAGGAATCTTCTGGAAATGAAACCCCACAGC TCAACAAAAGCAAGTTTGGTTTATTTGAATGGTTTCATTAAATAAGTCTACAAAGGTAACAAACTGAAGCACAAAGCGCAATCTTACAAGAAGCGCAACA CCCAGAGTTAGTGCAAAATGTACAATTAACGGCTGCTGAGAAACCCCCAAAGTTTGAATTTTTATTTTTATTTTTAACAGTGTACATTGTTAAATAATGT AAGGAAAACATTTATAATTCAGAAAGTTTTTAATGTGGAAAAAGATACTCAAAGTGTACTATTAACACAAAAATAATTTAAACAGTTCAGTAGCACTAGT TCATTCCTCTAGGCTTGTTATTTCATACACAGGAGGATGAAATCAACAGTAAATAACACCAGTTCTGACTTTTTTTGTGTATGAGTGAAAAGATGTCAAA ATTATTATTAAACCCAAATAACAAATGATAATGTGCTGCATGAGGCTCCACTGCTCCTCTGGTATAAAGTATGTTTCACTCTTGGGTGGCCCCTTTCTCA GCTACTCAGCCCCTTGTTGGTTCTATCCTTACTCTCTCCACAAGAAATACAAGCAGAGAATAAATAAATCATTTTAAAGCAGGGGTGGAAGCCCTTGCCC TTTTCCTTTCTACCTTTTAAAAACCTTCAGCTCTCATTTGCATATTTAGGGCCGTAGACCAACTTACTTACTTACTAAGGGAAGCAGGGCCCTTAAGGAA TGAACAGGGTACAGGAGCCTCCAGCCTCTTTCTGTGGTAGTGGTGGTGGTGGTGGTGATTCCCAGAATATGCCATTGACGTGGGTAAAAGGTTGGAAATA TTTTCAGTTTCCCAACTAAGAGTCAAGAAGCACCCCAAAATTAAGAGTCCTAGCTTCTTTCTTTGTCTTCAGAGATAACAAAGATCCTAAACATAGCCAG TTAGGACAGTATTTCCGGAAAGCTCTGAAAATAATTAATAACTACCCATTACAAGGAGGCATAGCACTGAATAACACTGATTGGTTGCAGTTATTACTTA CGGGGAGTATTATTTATTTGAAAATGTGAACATATTAGTTGTACTTCTAGAAATAAAGGTGGCACAATAGATTATGATCCTGCAACCATTTGGACATCAT AAAGGAAATGAGTAGGAATAAGTATTCTTTCCTAGAATGATTAATTCAGATGTGGAAAGATAGATAGATTAACTTTCCACAAAATGTAGTACCCCAAGCT GAGAAATGCATGGTACATGTGCAACAAGAGGAAAGCAATCCAAACAGTTTTTCTGAGAGCAATTTTCTCCATCTGTGACTTTTCACTATCAGGTTCCCCA ACTTGAAAACTAGCCCCCCACCCCCTTCTCTTTATGTTCAGTTTCTTCTAGGTCACGTCTGTTCAAGCCTAGCTCAAATAAGAAAGGTTTATATGACAGT CACTGGCCAGGCATCACGTTTGTTTCCTCATTCTTTAGTCTTTGTCTTTCACCCAGCACACACCATTCTTTTAAGTTGTCCAATTTTGTTACAACACCCC CTCCTCACTATTCCCGAAACAGAACTTTCTGTTTTTAACCACTGATGCCAACCTAACTTCCCACCCTACTCATCTTACACCTGCTCTAGACAAAGTTGTT GAATGACTTAAAAAAATACTTTTTCAATTTCCCTTTGGCATAACCACTGCTGGACTGCTGGGGTCACATGGACCTACGATTTCCTTCCACATGCCCTTTA TTGATCTGGGGTCTGGTAAGTTGATGCTAATGGCAGAAGCAATAGAAGTCCAAGGCACTAAAATGTCCAGCAAGCAACACTGGGAAACAGAAGTATTCAA GTACAGTAATTTATACAATTTTACATTCACATTTATTTATCTAATACAATGGAAATACAAAGGAAAAAGTTAAAGTGTCTCAATAAGTAAAGGACAGAGC TAGCTTTTTTTGGAACTTTTTAAACGTTTCATATTTTACTCTTAAAACTACTGTTGTCCAACAACATTTTATATATCACAGTGTTTCCACATCAAAACTG ATGGCAAAGTTACATGTCCGCAGGGAACTTAATTTTTTTTTTTTCCTAATTGCTAATGCTGCAGTCAAAGCTGCTAATATCTTTTGCTTAAAGCACTAAT GACCTATCATAAGAAAGAGTTGCACCCCAGTACCCTGGTATTTTTCACTGGGTTCTAAAGAGCAAAGAAAAAATAAAAAAGAGAGGGGTAAAAAAAAGGG AGGAGGGCAGGGTAGGAGAGAGCCCAGTAGGTGGTTTTTGTCCTGTGGTTCGGATTCTTGAGGGAGTGAGGGATTCAGGGCATGTACCAAAAGCAAGAGC TCCACCCACTCGAAATTACAGATGGGTTTGATTTTACATTGAGTTCTCCAGCTTCCTTATGAACAAGACTTTCCTAGCCCTCATCCTCATTACTGAGTAT AGATAAACAGGAAAGCATGGTCTGAAGCTGCCCCTTGACAAAAAAGCTGTTCTTTCCTTCCTCTCCCATTCCAATAACTGGAGCGGGGGAGACTCTCCAT TCTGAGGGCTCAATGCAGTATTAAAAACATTCCCCTCTGGCAAGAAATAACCAGTTAAATGCAGCACTAGTAGTTAGTATATAGGCTACTTCCCACCCCC ACCCACCCCCTCAGCATTCCATCTCCTGCCCCCAGATCTATTCAGTTAATGTCTCTAGGTCTAAGTGGTCCGCAGGTTGGAGCCTGGGGGGTTGCTGATG GATTTCTGCAAATTGCTGATGTTGAAATTCTGCAAGGAGGCAGTTCCCACAGGTTGGAACTGGCCAAGTGGCTGTGGTGCTGGGCCACCCGTCCCACTCC ATTGAGCGCCAAATGGGGTAGCTGGAAAGCCATACTGCTGTGGGGGGCACATAGACTTGGTGAAGTGACCTAGAGAGGTAGCTGATGCTGCAGAGATGGG GGCAGAGCCACCCAGGTTCGAGGTCATGGAGATGCACAGTTGCCCAGGTGCAGAGAACTTCCTTGCCATTCCAGGGCCCTGAAAACAAAACCACCACGTG TCAAGTCTGGTGTGGGCCATGGTATAAAATGTGTGGCGCTAACATATACTTCTAGTATAATCCTGTAAGATACAGCAGAACAGTCTTCTGTAGTATTTAT TGGCTCTTTATATTTACCAAGTAAGGGTGAAAATAATCCTCATGAGAGGGAGGGATGTCATGTGCGTGTGTGTCTGTGTGTGTGTAGTTGAGGATGACGA AGG

RAD52.u.f.18 (forward primer)
RAD52.u.r.18 (reverse primer)
Intron retention

Frame 0

RDARAPFPFSSARPPSKISSPCP*PRSSSLCAP RAARGAGQPESRCLGLRKQFLEDVTAILLLAAAQ CYALDSASTQQKSTRPSRRP*GRGWAQNT*VAA WLAEARRCATLRVIG*LIWPMRCLVTMAGHTPS RSRMWILLTSTMASSTWESVHL*GSS*RMVHIMK MLVMVLVRASSPRLYLWRRQGRRR*QTG*SEPS GFPCWE*VVESCTLSSPCTQSCVPEAFPLLLRV HRQHPPLRAPQQSSATHRSFGNALGNCILDKDYL RSLNKLPRQLPLEVDLTKAKRQDLEPSVEEARY NSCRPNMALGHPQLQQVTSPSRPSHAVIPADQD CSSRSLSSSAVESEATHQRKLRQKQLQQQFRERM EKQQVRVSTPSAEKSEAAPPAPPVTHSTPVTVS EPLLEKDFLAGVTQELISKYLEMLRLLKTTLKS GL*LPMQGMVWSSPRLEQTQPRPLTH*P*TTRW* PRTGLHTAFATRNHKQNLDLGTSKLIALTNAQQ ETGNLIGRART*RKGNMIHLN*GSGHIIGLCHK GTLENYFLVMKLFIAAGE*TSLRFILLHSEPYQE DLTESPLQLELSTFEKLVHHSSRERLWTDEYLF FGL*GFPLFITELLC**RWTF*ESPMAPCPQAI *ARLGPKHLPQQLSTFSCFA*RLPSFLYGPCL*C CPH*ALWITTGKRLEQCFSLLCINPGYNLHLI* IIYGFMTLQSCIVSRLTFFPKEGNNHLRPRKKA VFCFFFFFFF*DGVWLCCPDWSSVVQTQLSPQPL GPK*YSHLCLTKYRDYWCEPLCLARKGIFEKAN RIPY*QNRIYIYCLSEMLETRIVLHFLNICIHI MRPWGWDPSLNVEFTCVSCICLIHIILCMKQSFC IRRYTAAEEGWVFFSLRVAA*TVVCLVLCDLSH EVTCGIFHFWHHVSAQKFSDLRAFQLGMLKRNC FYFPISGVRCNPP*P*IGFS*CSDVS*FFPQNNE VCSLTTFFLRGGVWLCSAGWSAAVPS*LIAASA CQDSSNSPASGSRVAGITDTHHDARLIFVFLVE ETGFLHVGQAGLKLTSSDLPTSASQSKKY*NYRH EPLRPANLIIFLDVLFWAGLWLILKQ*LPIFWL RPIWEAVNLR*GSLKVLAGSRPFLFLSKLRRQQ RSSQELGIQL*IALNSK*CQ*ECEGIFWK*NPTA QQKQVWFI*MVSLNKSTKVTN*STKRNLTRSAT PRVSAKCTINGC*ETPKV*IFIFIFNSVHC*IM *GKHL*FRKFLMWKKILKVYY*HKNNLNSSVALV HSSRLVISYTGG*NQQ*ITPVLTFFVYE*KDVK IIIKPK*QMIMCCMRLHCSSGIKYVSLLGGPFL SYSAPCWFYPYSLHKKYKQRINKSF*SRGGSPCP FPFYLLKTFSSHLHI*GRRPTYLLTKGSRALKE *TGYRSLQPLSVVVVVVVVIPRICH*RG*KVGN IFSFPTKSQEAPQN*ES*LLSLSSEITKILNIAS *DSISGKL*K*LITTHYKEA*H*ITLIGCSYYL RGVLFI*KCEHISCTSRNKGGTIDYDPATIWTS *RK*VGISILS*ND*FRCGKIDRLTFHKM*YPKL RNAWYMCNKRKAIQTVFLRAIFSICDFSLSGSP T*KLAPHPLLFMFSFF*VTSVQA*LK*ERFI*Q SLARHHVCFLIL*SLSFTQHTPFF*VVQFCYNTP SSLFPKQNFLFLTTDANLTSHPTHLTPALDKVV E*LKKILFQFPFGITTAGLLGSHGPTISFHMPF IDLGSGKLMLMAEAIEVQGTKMSSKQHWETEVFK YSNLYNFTFTFIYLIQWKYKGKS*SVSISKGQS *LFLELFKRFIFYS*NYCCPTTFYISQCFHIKT DGKVTCPQGT*FFFFPNC*CCSQSC*YLLLKALM TYHKKELHPSTLVFFTGF*RAKKK*KREG*KKG RRAG*ERAQ*VVFVLWFGFLRE*GIQGMYQKQE LHPLEITDGFDFTLSSPASL*TRLS*PSSSLLSI DKQESMV*SCPLTKKLFFPSSPIPITGAGETLH SEGSMQY*KHSPLARNNQLNAALVVSI*ATSHP HPPPQHSISCPQIYSVNVSRSKWSAGWSLGGC*W ISANC*C*NSARRQFPQVGTGQVAVVLGHPSHS IERQMG*LESHTAVGGT*TW*SDLER*LMLQRW GQSHPGSRSWRCTVAQVQRTSLPFQGPENKTTTC QVWCGPWYKMCGANIYF*YNPVRYSRTVFCSIY WLFIFTK*G*K*SS*EGGMSCACVSVCV*LRMT K

Frame 1

ETLAHPSHSPLRGLHLRSLPLVHSLDRAPCVHR ALPEAQVNQNQDVWD*GSNSWRT*QPSCCWRRL SVMLWTVPVHSRRVPGHPEGPEAEAGPRIHK*PH GWRRPEGVLH*GSSGN*SGQ*DVWLQWLGTLHH AAECGFC*PQQWQVLRGSLCICEGPAEGWFIS* RCWLWC**GPQVQGFIFGEGKEGGGDRRAEASPQ ASLAGSKW*NPVLSLLRALSHVCRRPSHSYCEC TDSTLLSVLLSSPPLRTGVLGMHLETVFWTKTT *DH*ISFHASCLLKWI*LKRRDKILNRLWRRQDT TAADRTWPWDTHSCSR*PPLPDPAML*YRRTRT AAPEA*AHPPWRARPRTSGSSGRSSCSSSSGSG WRSSRFESPRRQLRRVRQRLRPLL*RTALL*LSQ NHSWRKTSLQE*LKN*SVNTLKCLDS*RQL*KV GCDSRCRGWCGQALV*SRPSPDL*HISLEQPDG DPEQDSTQRLPPETTSKIWILGPPNL*R*PTHNR KLGIS*EEPGHEEKEI*SILTEAQAT*LDSVTK GLWKTTFWS*NCSSLLENERHCDLSCFILNLIK RI*LRAHCS*S*ALLKSLSITLVGRGSGQMNTFS SACEASHYLLLNYYVNEDGHFRNHQWLLALKQY RPDLVLSTCLSNCLHSVVLHNVCLLSFTVHAFN VAHIKHCGSRQEKGWSSAFHYFVSIQATIFI*YK *FMDL*HYNPALFQD*HFFLRKEIII*DHEKRL FFVFFFFFFFETGSGCVALTGVQWCKHSSLHNL LGPSDTPTSALQNTGITGVSHCVWPEKAFLRKQI VYLINKIEYIYIAYLKCLKPELFCIF*IFVYT* *DLGDGTQV*TWNSPVFRVYASYT*FCA*NRVF V*EDTLQLKRAGFFFLLGSLHKLLYAWCFATCHT RSRVEFSTSGITSVLRNFLISEHFN*GCSNATV STSPFQV*DVTHLDHKLAFHSAQMFHNSSPKIM KFAV*QLFF*EGGSGSVAQAGVQRCHLDSLQPPP ARIQAILLPQAPE*LGLQTPTTTPD*FLYF**R RRGFSMLARLVSN*PQVICPPRPPKVKSTRIIG MSHCAQPI**FF*TYYFGLGCG*S*SSDFLFFGS DPFGKL*T*DKEV*KY*PAADRFYSFRS*GDSK GAPRN*GSSYKLHLTQNSASENVKESSGNETPQ LNKSKFGLFEWFH*ISLQR*QTEAQSAILQEAQH PELVQNVQLTAAEKPPKFEFLFLFLTVYIVK*C KENIYNSESF*CGKRYSKCTINTKII*TVQ*H* FIPLGLLFHTQEDEINSK*HQF*LFLCMSEKMSK LLLNPNNK**CAA*GSTAPLV*SMFHSWVAPFS ATQPLVGSILTLSTRNTSRE*INHFKAGVEALA LFLSTF*KPSALICIFRAVDQLTYLLREAGPLRN EQGTGASSLFLW*WWWWW*FPEYAIDVGKRLEI FSVSQLRVKKHPKIKSPSFFLCLQR*QRS*T*P VRTVFPESSENN**LPITRRHSTE*H*LVAVITY GEYYLFENVNILVVLLEIKVAQ*IMILQPFGHH KGNE*E*VFFPRMINSDVER*ID*LSTKCSTPS *EMHGTCATRGKQSKQFF*EQFSPSVTFHYQVPQ LEN*PPTPFSLCSVSSRSRLFKPSSNKKGLYDS HWPGITFVSSFFSLCLSPSTHHSFKLSNFVTTP PPHYSRNRTFCF*PLMPT*LPTLLILHLL*TKLL NDLKKYFFNFPLA*PLLDCWGHMDLRFPSTCPL LIWGLVS*C*WQKQ*KSKALKCPASNTGKQKYS STVIYTILHSHLFI*YNGNTKEKVKVSQ*VKDRA SFFWNFLNVSYFTLKTTVVQQHFIYHSVSTSKL MAKLHVRRELNFFFFLIANAAVKAANIFCLKH* *PIIRKSCTPVPWYFSLGSKEQRKNKKERGKKKG GGQGRREPSRWFLSCGSDS*GSEGFRACTKSKS STHSKLQMGLILH*VLQLPYEQDFPSPHPHY*V *INRKAWSEAAP*QKSCSFLPLPFQ*LERGRLSI LRAQCSIKNIPLWQEITS*MQH**LVYRLLPTP THPLSIPSPAPRSIQLMSLGLSGPQVGAWGVAD GFLQIADVEILQGGSSHRLELAKWLWCWATRPTP LSAKWGSWKAILLWGAHRLGEVT*RGS*CCRDG GRATQVRGHGDAQLPRCRELPCHSRALKTKPPR VKSGVGHGIKCVALTYTSSIIL*DTAEQSSVVFI GSLYLPSKGENNPHEREGCHVRVCLCVCS*G*R R

Frame 2

RRSRTLPILLCAASI*DLFPLSIA*IELPVCTA RCPRRRSTRIKMSGTEEAILGGRDSHPAAGGGS VLCFGQCQYTAEEYQAIQKALRQRLGPEYISSR MAGGGQKVCYIEGHRVINLANEMFGYNGWAHSIT QQNVDFVDLNNGKFYVGVCAFVRVQLKDGSYHE DVGYGVSEGLKSKALSLEKARKEAVTDGLKRAL RLPLLGVSGRILYSLFSVHSVMCAGGLPTPTASA QTAPSSPCSSAVLRYAQEFWECTWKLYSGQRLP EITK*ASTPVAS*SGFN*SEETRS*TVCGGGKI QQLPTEHGPGTPTAAAGDLPFQTQPCCDTGGPGL QLPKPELIRRGERGHAPAEAPAEAAAAAVPGAD GEAAGSSLHAVS*EE*GSASGPSCDAQHSCNCL RTTPGERLPCRSDSRINQ*IP*NA*TLEDNSEKW AVTPDAGDGVVKPSSRADPAQTSDTLALNNQMV TQNRTPHSVCHQKPQAKSGSWDLQTYSADQRTT GNWESHRKSQDMKKRKYDPS*LRLRPHNWTLSQR DFGKLLFGHEIVHRCWRMNVIAIYLASF*TLSR GSD*EPTAVRAEHF*KACPSL**GEALDR*IPF LRLVRLPTIYY*TIMLMKMDILGITNGSLPSSNI GQTWS*APASAIVYIQLFCITSAFFPLRSMPLM LPTLSTVDHDRKKVGAVLFTTLYQSRLQSSFNI NNLWIYDITILHCFKTDIFS*GRK*SSKTTKKGC FLFFFFFFFLRRGLAVLP*LEFSGANTALSTTS WAQVILPPLPYKIQGLLV*ATVSGQKRHF*ESK SYTLLTK*NIYILLI*NA*NQNCFAFFEYLYTHN ETLGMGPKSERGIHLCFVYMPHTHNFVHETEFL YKKIHCS*RGLGFFFS*GRCINCCMPGALRLVT RGHVWNFPLLASRQCSEIF*SQSISIRDAQTQLF LLPHFRCEM*PTLTINWLFIVLRCFIILPPK** SLQFNNFFFERGGLAL*RRLECSGAILTHCSLR LPGFKQFSCLRLPSSWDYRHPPRRPTNFCIFSRG DGVSPCWPGWSQTDLK*SAHLGLPK*KVLEL*A *AIAPSQFNNFFRRIILGWAVANLEAVTSYFLA QTHLGSCEPEIRKSESTSRQPTVSIPFEVKETAK ELPGIRDPVINCT*LKIVPVRM*RNLLEMKPHS STKASLVYLNGFIK*VYKGNKLKHKAQSYKKRN TQS*CKMYN*RLLRNPQSLNFYFYF*QCTLLNNV RKTFIIQKVFNVEKDTQSVLLTQK*FKQFSSTS SFL*ACYFIHRRMKSTVNNTSSDFFCV*VKRCQ NYY*TQITNDNVLHEAPLLLWYKVCFTLGWPLSQ LLSPLLVLSLLSPQEIQAENK*IILKQGWKPLP FSFLPFKNLQLSFAYLGP*TNLLTY*GKQGP*G MNRVQEPPASFCGSGGGGGDSQNMPLTWVKGWKY FQFPN*ESRSTPKLRVLASFFVFRDNKDPKHSQ LGQYFRKALKIINNYPLQGGIALNNTDWLQLLL TGSIIYLKM*TY*LYF*K*RWHNRL*SCNHLDII KEMSRNKYSFLE*LIQMWKDR*INFPQNVVPQA EKCMVHVQQEESNPNSFSESNFLHL*LFTIRFP NLKTSPPPPSLYVQFLLGHVCSSLAQIRKVYMTV TGQASRLFPHSLVFVFHPAHTILLSCPILLQHP LLTIPETELSVFNH*CQPNFPPYSSYTCSRQSC *MT*KNTFSISLWHNHCWTAGVTWTYDFLPHALY *SGVW*VDANGRSNRSPRH*NVQQATLGNRSIQ VQ*FIQFYIHIYLSNTMEIQRKKLKCLNK*RTE LAFFGTF*TFHILLLKLLLSNNILYITVFPHQN* WQSYMSAGNLIFFFS*LLMLQSKLLISFA*STN DLS*ERVAPQYPGIFHWVLKSKEKIKKRGVKKR EEGRVGESPVGGFCPVVRILEGVRDSGHVPKARA PPTRNYRWV*FYIEFSSFLMNKTFLALILITEY R*TGKHGLKLPLDKKAVLSFLSHSNNWSGGDSP F*GLNAVLKTFPSGKK*PVKCSTSS*YIGYFPPP PTPSAFHLLPPDLFS*CL*V*VVRRLEPGGLLM DFCKLLMLKFCKEAVPTGWNWPSGCGAGPPVPL H*APNGVAGKPYCCGGHIDLVK*PREVADAAEMG AEPPRFEVMEMHSCPGAENFLAIPGP*KQNHHV SSLVWAMV*NVWR*HILLV*SCKIQQNSLL*YL LALYIYQVRVKIILMRGRDVMCVCVCVCVVEDDE

Sequence RAD52.mAug05

TGTTATGCTTTGGACAGTGCCAGTACACAGCAGAAGAGTACCAGGCCATCCAGAAGGCCCTGAGGCAGAGGCTGGGCCCAGAATACATAAGTAGCCGCAT GGCTGGCGGAGGCCAGAAGGTGTGCTACATTGAGGGTCATCGGGTAATTAATCTGGCCAATGAGATGTTTGGTTACAATGGCTGGGCACACTCCATCACG CAGCAGAATGTGGATTTTGTTGACCTCAACAATGGCAAGTTCTACGTGGGAGTCTGTGCATTTGTGAGGGTCCAGCTGAAGGTGAGGGGATGGAGTAGAC CTGCTGCCCGGAAGGATCAGTGGGTGGTAGGAGAAGGATGGTTCATATCATGAAGATGTTGGTTATGGTGTTAGTGAGGGCCTCAAGTCCAAGGCTTTAT CTTTGGAGAAGGCAAGGAAGGAGGCGGTGACAGACGGGCTGAAGCGAGCCCTCAGGCTTCCCTTGCTGGGAGTAAGTGGTAGAATCCTGTACTCTCTCTT CTCCGTGCACTCAGTCATGTGTGCCGGAGGCCTTCCCACTCCTACTGCGAGTGCACAGACAGCACCCTCCTCTCCGTGCTCCTCAGCAGTCCTCCGCTAC GCACAGGAGTTTTGGGAATGCACTTGGAAACTGTATTCTGGACAAAGACTACCTGAGATCACTAAATAAGCTTCCACGCCAGTTGCCTCTTGAAGTGGAT TTAACTAAAGCGAAGAGACAAGATCTTGAACCGTCTGTGGAGGAGGCAAGATACAACAGCTGCCGACCGAACATGGCCCTGGGACACCCACAGCTGCAGC AGGTGACCTCCCCTTCCAGACCCAGCCATGCTGTGATACCGGCGGACCAGGACTGCAGCTCCCGGTATGC

RAD52.u.f.18 (forward primer)
RAD52.u.r.18 (reverse primer)
Intron retention

Frame 0

CYALDSASTQQKSTRPSRRP*GRGWAQNT*VAA WLAEARRCATLRVIG*LIWPMRCLVTMAGHTPSR SRMWILLTSTMASSTWESVHL*GSS*R*GDGVD LLPGRISGW*EKDGSYHEDVGYGVSEGLKSKAL SLEKARKEAVTDGLKRALRLPLLGVSGRILYSLF SVHSVMCAGGLPTPTASAQTAPSSPCSSAVLRY AQEFWECTWKLYSGQRLPEITK*ASTPVAS*SG FN*SEETRS*TVCGGGKIQQLPTEHGPGTPTAAA GDLPFQTQPCCDTGGPGLQLPVC

Frame 1

VMLWTVPVHSRRVPGHPEGPEAEAGPRIHK*PH GWRRPEGVLH*GSSGN*SGQ*DVWLQWLGTLHH AAECGFC*PQQWQVLRGSLCICEGPAEGEGME*T CCPEGSVGGRRRMVHIMKMLVMVLVRASSPRLY LWRRQGRRR*QTG*SEPSGFPCWE*VVESCTLS SPCTQSCVPEAFPLLLRVHRQHPPLRAPQQSSAT HRSFGNALGNCILDKDYLRSLNKLPRQLPLEVD LTKAKRQDLEPSVEEARYNSCRPNMALGHPQLQ QVTSPSRPSHAVIPADQDCSSRY

Frame 2

LCFGQCQYTAEEYQAIQKALRQRLGPEYISSRM AGGGQKVCYIEGHRVINLANEMFGYNGWAHSIT QQNVDFVDLNNGKFYVGVCAFVRVQLKVRGWSR PAARKDQWVVGEGWFIS*RCWLWC**GPQVQGFI FGEGKEGGGDRRAEASPQASLAGSKW*NPVLSL LRALSHVCRRPSHSYCECTDSTLLSVLLSSPPL RTGVLGMHLETVFWTKTT*DH*ISFHASCLLKWI *LKRRDKILNRLWRRQDTTAADRTWPWDTHSCS R*PPLPDPAML*YRRTRTAAPGM

Sequence RAD52.eAug05

AGAGACGCTCGCGCACCCTTCCCATTCTCCTCTGCGCGGCCTCCATCTAAGATCTCTTCCCCTTGTCCATAGCCTAGATCGAGCTCCCTGTGTGCACCGC GCGCTGCCCGAGGCGCAGGTCAACCAGAATCAAGATGTCTGGGACTGAGGAAGCAATTCTTGGAGGACGTGACAGCCATCCTGCTGCTGGCGGCGGCTCA GTGTTATGCTTTGGACAGTGCCAGTACACAGCAGAAGAGTACCAGGCCATCCAGAAGGCCCTGAGGCAGAGGCTGGGCCCAGAATACATAAGTAGCCGCA TGGCTGGCGGAGGCCAGAAGGTGTGCTACATTGAGGGTCATCGGGTAATTAATCTGGCCAATGAGATGTTTGGTTACAATGGCTGGGCACACTCCATCAC GCAGCAGAATGTGGATTTTGTTGACCTCAACAATGGCAAGTTCTACGTGGGAGTCTGTGCATTTGTGAGGGTCCAGCTGAAGGTGAGGGGATGGAGTAGA CCTGCTGCCCGGAAGGATCAGTGGGTGGTAGGAGAAGGATGGTTCATATCATGAAGATGTTGGTTATGGTGTTAGTGAGGGCCTCAAGTCCAAGGCTTTA TCTTTGGAGAAGGCAAGGAAGGAGGCGGTGACAGACGGGCTGAAGCGAGCCCTCAGGTGAGCGCGGGGCTGGGAGTCTAACCTGCGTCATTTCAGCTCTT GCTTCAGAGCAGATTTCGATTTTTTTTGGAAGAGGTAACACATCCACATGGTTCAAAAATCCAAGCAGCGTGGAAGGTAATTCTCACCCCTGCCCTGGTT CCTGTCTGCCGTTTTCCCACTTTCGTTAGTATTTTTTCAATGTTTCTGTTTCAGGCTTCCCTTGCTGGGAGTAAGTGGTAGAATCCTGTACTCTCTCTTC TCCGTGCACTCAGTCATGTGTGCCGGAGGCCTTCCCACTCCTACTGCGAGTGCACAGACAGCACCCTCCTCTCCGTGCTCCTCAGCAGTCCTCCGCTACG CACAGGAGTTTTGGGAATGCACTTGGAAACTGTATTCTGGACAAAGACTACCTGAGATCACTAAATAAGCTTCCACGCCAGTTGCCTCTTGAAGTGGATT TAACTAAAGCGAAGAGACAAGATCTTGAACCGTCTGTGGAGGAGGCAAGATACAACAGCTGCCGACCGAACATGGCCCTGGGACACCCACAGCTGCAGCA GGTGACCTCCCCTTCCAGACCCAGCCATGCTGTGATACCGGCGGACCAGGACTGCAGCTCCCGAAGCCTGAGCTCATCCGCCGTGGAGAGCGAGGCCACG CACCAGCGGAAGCTCCGGCAGAAGCAGCTGCAGCAGCAGTTCCGGGAGCGGATGGAGAAGCAGCAGGTTCGAGTCTCCACGCCGTCAGCTGAGAAGAGTG AGGCAGCGCCTCCGGCCCCTCCTGTGACGCACAGCACTCCTGTAACTGTCTCAGAACCACTCCTGGAGAAAGACTTCCTTGCAGGAGTGACTCAAGAATT AATCAGTAAATACCTTGAAATGCTTAGACTCTTGAAGACAACTCTGAAAAGTGGGCTGTGACTCCCGATGCAGGGGATGGTGTGGTCAAGCCCTCGTCTA GAGCAGACCCAGCCCAGACCTCTGACACATTAGCCTTGAACAACCAGATGGTGACCCAGAACAGGACTCCACACAGCGTTTGCCACCAGAAACCACAAGC AAAATCTGGATCTTGGGACCTCCAAACTTATAGCGCTGACCAACGCACAACAGGAAACTGGGAATCTCATAGGAAGAGCCAGGACATGAAGAAAAGGAAA TATGATCCATCTTAACTGAGGCTCAGGCCACATAATTGGACTCTGTCACAAAGGGACTTTGGAAAACTACTTTTTGGTCATGAAATTGTTCATCGCTGCT GGAGAATGAACGTCATTGCGATTTATCTTGCTTCATTCTGAACCTTATCAAGAGGATCTGACTGAGAGCCCACTGCAGTTAGAGCTGAGCACTTTTGAAA AGCTTGTCCATCACTCTAGTAGGGAGAGGCTCTGGACAGATGAATACCTTTTCTTCGGCTTGTGAGGCTTCCCACTATTTATTACTGAACTATTATGTTA ATGAAGATGGACATTTTAGGAATCACCAATGGCTCCTTGCCCTCAAGCAATATAGGCCAGACTTGGTCCTAAGCACCTGCCTCAGCAATTGTCTACATTC AGTTGTTTTGCATAACGTCTGCCTTCTTTCCTTTACGGTCCATGCCTTTAATGTTGCCCACATTAAGCACTGTGGATCACGACAGGAAAAAGGTTGGAGC AGTGCTTTTCACTACTTTGTATCAATCCAGGCTACAATCTTCATTTAATATAAATAATTTATGGATTTATGACATTACAATCCTGCATTGTTTCAAGACT GACATTTTTTCCTAAGGAAGGAAATAATCATCTAAGACCACGAAAAAAGGCTGTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTGAGACGGGGTCTGGCTG TGTTGCCCTGACTGGAGTTCAGTGGTGCAAACACAGCTCTCTCCACAACCTCTTGGGCCCAAGTGATACTCCCACCTCTGCCTTACAAAATACAGGGATT ACTGGTGTGAGCCACTGTGTCTGGCCAGAAAAGGCATTTTTGAGAAAGCAAATCGTATACCTTATTAACAAAATAGAATATATATATATTGCTTATCTGA AATGCTTGAAACCAGAATTGTTTTGCATTTTTTGAATATTTGTATACACATAATGAGACCTTGGGGATGGGACCCAAGTCTGAACGTGGAATTCACCTGT GTTTCGTGTATATGCCTCATACACATAATTTTGTGCATGAAACAGAGTTTTTGTATAAGAAGATACACTGCAGCTGAAGAGGGCTGGGTTTTTTTTTCTC TTAGGGTCGCTGCATAAACTGTTGTATGCCTGGTGCTTTGCGACTTGTCACACGAGGTCACGTGTGGAATTTTCCACTTCTGGCATCACGTCAGTGCTCA GAAATTTTCTGATCTCAGAGCATTTCAATTAGGGATGCTCAAACGCAACTGTTTCTACTTCCCCATTTCAGGTGTGAGATGTAACCCACCTTGACCATAA ATTGGCTTTTCATAGTGCTCAGATGTTTCATAATTCTTCCCCCAAAATAATGAAGTTTGCAGTTTAACAACTTTTTTTTTGAGAGGGGGGGTCTGGCTCT GTAGCGCAGGCTGGAGTGCAGCGGTGCCATCTTGACTCATTGCAGCCTCCGCCTGCCAGGATTCAAGCAATTCTCCTGCCTCAGGCTCCCGAGTAGCTGG GATTACAGACACCCACCACGACGCCCGACTAATTTTTGTATTTTTAGTAGAGGAGACGGGGTTTCTCCATGTTGGCCAGGCTGGTCTCAAACTGACCTCA AGTGATCTGCCCACCTCGGCCTCCCAAAGTAAAAAGTACTAGAATTATAGGCATGAGCCATTGCGCCCAGCCAATTTAATAATTTTTTTAGACGTATTAT TTTGGGCTGGGCTGTGGCTAATCTTGAAGCAGTGACTTCCTATTTTTTGGCTCAGACCCATTTGGGAAGCTGTGAACCTGAGATAAGGAAGTCTGAAAGT ACTAGCCGGCAGCCGACCGTTTCTATTCCTTTCGAAGTTAAGGAGACAGCAAAGGAGCTCCCAGGAATTAGGGATCCAGTTATAAATTGCACTTAACTCA AAATAGTGCCAGTGAGAATGTGAAGGAATCTTCTGGAAATGAAACCCCACAGCTCAACAAAAGCAAGTTTGGTTTATTTGAATGGTTTCATTAAATAAGT CTACAAAGGTAACAAACTGAAGCACAAAGCGCAATCTTACAAGAAGCGCAACACCCAGAGTTAGTGCAAAATGTACAATTAACGGCTGCTGAGAAACCCC CAAAGTTTGAATTTTTATTTTTATTTTTAACAGTGTACATTGTTAAATAATGTAAGGAAAACATTTATAATTCAGAAAGTTTTTAATGTGGAAAAAGATA CTCAAAGTGTACTATTAACACAAAAATAATTTAAACAGTTCAGTAGCACTAGTTCATTCCTCTAGGCTTGTTATTTCATACACAGGAGGATGAAATCAAC AGTAAATAACACCAGTTCTGACTTTTTTTGTGTATGAGTGAAAAGATGTCAAAATTATTATTAAACCCAAATAACAAATGATAATGTGCTGCATGAGGCT CCACTGCTCCTCTGGTATAAAGTATGTTTCACTCTTGGGTGGCCCCTTTCTCAGCTACTCAGCCCCTTGTTGGTTCTATCCTTACTCTCTCCACAAGAAA TACAAGCAGAGAATAAATAAATCATTTTAAAGCAGGGGTGGAAGCCCTTGCCCTTTTCCTTTCTACCTTTTAAAAACCTTCAGCTCTCATTTGCATATTT AGGGCCGTAGACCAACTTACTTACTTACTAAGGGAAGCAGGGCCCTTAAGGAATGAACAGGGTACAGGAGCCTCCAGCCTCTTTCTGTGGTAGTGGTGGT GGTGGTGGTGATTCCCAGAATATGCCATTGACGTGGGTAAAAGGTTGGAAATATTTTCAGTTTCCCAACTAAGAGTCAAGAAGCACCCCAAAATTAAGAG TCCTAGCTTCTTTCTTTGTCTTCAGAGATAACAAAGATCCTAAACATAGCCAGTTAGGACAGTATTTCCGGAAAGCTCTGAAAATAATTAATAACTACCC ATTACAAGGAGGCATAGCACTGAATAACACTGATTGGTTGCAGTTATTACTTACGGGGAGTATTATTTATTTGAAAATGTGAACATATTAGTTGTACTTC TAGAAATAAAGGTGGCACAATAGATTATGATCCTGCAACCATTTGGACATCATAAAGGAAATGAGTAGGAATAAGTATTCTTTCCTAGAATGATTAATTC AGATGTGGAAAGATAGATAGATTAACTTTCCACAAAATGTAGTACCCCAAGCTGAGAAATGCATGGTACATGTGCAACAAGAGGAAAGCAATCCAAACAG TTTTTCTGAGAGCAATTTTCTCCATCTGTGACTTTTCACTATCAGGTTCCCCAACTTGAAAACTAGCCCCCCACCCCCTTCTCTTTATGTTCAGTTTCTT CTAGGTCACGTCTGTTCAAGCCTAGCTCAAATAAGAAAGGTTTATATGACAGTCACTGGCCAGGCATCACGTTTGTTTCCTCATTCTTTAGTCTTTGTCT TTCACCCAGCACACACCATTCTTTTAAGTTGTCCAATTTTGTTACAACACCCCCTCCTCACTATTCCCGAAACAGAACTTTCTGTTTTTAACCACTGATG CCAACCTAACTTCCCACCCTACTCATCTTACACCTGCTCTAGACAAAGTTGTTGAATGACTTAAAAAAATACTTTTTCAATTTCCCTTTGGCATAACCAC TGCTGGACTGCTGGGGTCACATGGACCTACGATTTCCTTCCACATGCCCTTTATTGATCTGGGGTCTGGTAAGTTGATGCTAATGGCAGAAGCAATAGAA GTCCAAGGCACTAAAATGTCCAGCAAGCAACACTGGGAAACAGAAGTATTCAAGTACAGTAATTTATACAATTTTACATTCACATTTATTTATCTAATAC AATGGAAATACAAAGGAAAAAGTTAAAGTGTCTCAATAAGTAAAGGACAGAGCTAGCTTTTTTTGGAACTTTTTAAACGTTTCATATTTTACTCTTAAAA CTACTGTTGTCCAACAACATTTTATATATCACAGTGTTTCCACATCAAAACTGATGGCAAAGTTACATGTCCGCAGGGAACTTAATTTTTTTTTTTTCCT AATTGCTAATGCTGCAGTCAAAGCTGCTAATATCTTTTGCTTAAAGCACTAATGACCTATCATAAGAAAGAGTTGCACCCCAGTACCCTGGTATTTTTCA CTGGGTTCTAAAGAGCAAAGAAAAAATAAAAAAGAGAGGGGTAAAAAAAAGGGAGGAGGGCAGGGTAGGAGAGAGCCCAGTAGGTGGTTTTTGTCCTGTG GTTCGGATTCTTGAGGGAGTGAGGGATTCAGGGCATGTACCAAAAGCAAGAGCTCCACCCACTCGAAATTACAGATGGGTTTGATTTTACATTGAGTTCT CCAGCTTCCTTATGAACAAGACTTTCCTAGCCCTCATCCTCATTACTGAGTATAGATAAACAGGAAAGCATGGTCTGAAGCTGCCCCTTGACAAAAAAGC TGTTCTTTCCTTCCTCTCCCATTCCAATAACTGGAGCGGGGGAGACTCTCCATTCTGAGGGCTCAATGCAGTATTAAAAACATTCCCCTCTGGCAAGAAA TAACCAGTTAAATGCAGCACTAGTAGTTAGTATATAGGCTACTTCCCACCCCCACCCACCCCCTCAGCATTCCATCTCCTGCCCCCAGATCTATTCAGTT AATGTCTCTAGGTCTAAGTGGTCCGCAGGTTGGAGCCTGGGGGGTTGCTGATGGATTTCTGCAAATTGCTGATGTTGAAATTCTGCAAGGAGGCAGTTCC CACAGGTTGGAACTGGCCAAGTGGCTGTGGTGCTGGGCCACCCGTCCCACTCCATTGAGCGCCAAATGGGGTAGCTGGAAAGCCATACTGCTGTGGGGGG CACATAGACTTGGTGAAGTGACCTAGAGAGGTAGCTGATGCTGCAGAGATGGGGGCAGAGCCACCCAGGTTCGAGGTCATGGAGATGCACAGTTGCCCAG GTGCAGAGAACTTCCTTGCCATTCCAGGGCCCTGAAAACAAAACCACCACGTGTCAAGTCTGGTGTGGGCCATGGTATAAAATGTGTGGCGCTAACATAT ACTTCTAGTATAATCCTGTAAGATACAGCAGAACAGTCTTCTGTAGTATTTATTGGCTCTTTATATTTACCAAGTAAGGGTGAAAATAATCCTCATGAGA GGGAGGGATGTCATGTGCGTGTGTGTCTGTGTGTGTGTAGTTGAGGATGACGAAGG

RAD52.u.f.18 (forward primer)
RAD52.u.r.18 (reverse primer)
Intron retention

Frame 0

RDARAPFPFSSARPPSKISSPCP*PRSSSLCAP RAARGAGQPESRCLGLRKQFLEDVTAILLLAAAQ CYALDSASTQQKSTRPSRRP*GRGWAQNT*VAA WLAEARRCATLRVIG*LIWPMRCLVTMAGHTPS RSRMWILLTSTMASSTWESVHL*GSS*R*GDGVD LLPGRISGW*EKDGSYHEDVGYGVSEGLKSKAL SLEKARKEAVTDGLKRALR*ARGWESNLRHFSS CFRADFDFFWKR*HIHMVQKSKQRGR*FSPLPWF LSAVFPLSLVFFQCFCFRLPLLGVSGRILYSLF SVHSVMCAGGLPTPTASAQTAPSSPCSSAVLRY AQEFWECTWKLYSGQRLPEITK*ASTPVAS*SGF N*SEETRS*TVCGGGKIQQLPTEHGPGTPTAAA GDLPFQTQPCCDTGGPGLQLPKPELIRRGERGH APAEAPAEAAAAAVPGADGEAAGSSLHAVS*EE* GSASGPSCDAQHSCNCLRTTPGERLPCRSDSRI NQ*IP*NA*TLEDNSEKWAVTPDAGDGVVKPSS RADPAQTSDTLALNNQMVTQNRTPHSVCHQKPQA KSGSWDLQTYSADQRTTGNWESHRKSQDMKKRK YDPS*LRLRPHNWTLSQRDFGKLLFGHEIVHRC WRMNVIAIYLASF*TLSRGSD*EPTAVRAEHF*K ACPSL**GEALDR*IPFLRLVRLPTIYY*TIML MKMDILGITNGSLPSSNIGQTWS*APASAIVYI QLFCITSAFFPLRSMPLMLPTLSTVDHDRKKVGA VLFTTLYQSRLQSSFNINNLWIYDITILHCFKT DIFS*GRK*SSKTTKKGCFLFFFFFFFLRRGLA VLP*LEFSGANTALSTTSWAQVILPPLPYKIQGL LV*ATVSGQKRHF*ESKSYTLLTK*NIYILLI* NA*NQNCFAFFEYLYTHNETLGMGPKSERGIHL CFVYMPHTHNFVHETEFLYKKIHCS*RGLGFFFS *GRCINCCMPGALRLVTRGHVWNFPLLASRQCS EIF*SQSISIRDAQTQLFLLPHFRCEM*PTLTI NWLFIVLRCFIILPPK**SLQFNNFFFERGGLAL *RRLECSGAILTHCSLRLPGFKQFSCLRLPSSW DYRHPPRRPTNFCIFSRGDGVSPCWPGWSQTDL K*SAHLGLPK*KVLEL*A*AIAPSQFNNFFRRII LGWAVANLEAVTSYFLAQTHLGSCEPEIRKSES TSRQPTVSIPFEVKETAKELPGIRDPVINCT*L KIVPVRM*RNLLEMKPHSSTKASLVYLNGFIK*V YKGNKLKHKAQSYKKRNTQS*CKMYN*RLLRNP QSLNFYFYF*QCTLLNNVRKTFIIQKVFNVEKD TQSVLLTQK*FKQFSSTSSFL*ACYFIHRRMKST VNNTSSDFFCV*VKRCQNYY*TQITNDNVLHEA PLLLWYKVCFTLGWPLSQLLSPLLVLSLLSPQE IQAENK*IILKQGWKPLPFSFLPFKNLQLSFAYL GP*TNLLTY*GKQGP*GMNRVQEPPASFCGSGG GGGDSQNMPLTWVKGWKYFQFPN*ESRSTPKLR VLASFFVFRDNKDPKHSQLGQYFRKALKIINNYP LQGGIALNNTDWLQLLLTGSIIYLKM*TY*LYF *K*RWHNRL*SCNHLDIIKEMSRNKYSFLE*LI QMWKDR*INFPQNVVPQAEKCMVHVQQEESNPNS FSESNFLHL*LFTIRFPNLKTSPPPPSLYVQFL LGHVCSSLAQIRKVYMTVTGQASRLFPHSLVFV FHPAHTILLSCPILLQHPLLTIPETELSVFNH*C QPNFPPYSSYTCSRQSC*MT*KNTFSISLWHNH CWTAGVTWTYDFLPHALY*SGVW*VDANGRSNR SPRH*NVQQATLGNRSIQVQ*FIQFYIHIYLSNT MEIQRKKLKCLNK*RTELAFFGTF*TFHILLLK LLLSNNILYITVFPHQN*WQSYMSAGNLIFFFS *LLMLQSKLLISFA*STNDLS*ERVAPQYPGIFH WVLKSKEKIKKRGVKKREEGRVGESPVGGFCPV VRILEGVRDSGHVPKARAPPTRNYRWV*FYIEF SSFLMNKTFLALILITEYR*TGKHGLKLPLDKKA VLSFLSHSNNWSGGDSPF*GLNAVLKTFPSGKK *PVKCSTSS*YIGYFPPPPTPSAFHLLPPDLFS *CL*V*VVRRLEPGGLLMDFCKLLMLKFCKEAVP TGWNWPSGCGAGPPVPLH*APNGVAGKPYCCGG HIDLVK*PREVADAAEMGAEPPRFEVMEMHSCP GAENFLAIPGP*KQNHHVSSLVWAMV*NVWR*HI LLV*SCKIQQNSLL*YLLALYIYQVRVKIILMR GRDVMCVCVCVCVVEDDE

Frame 1

ETLAHPSHSPLRGLHLRSLPLVHSLDRAPCVHR ALPEAQVNQNQDVWD*GSNSWRT*QPSCCWRRL SVMLWTVPVHSRRVPGHPEGPEAEAGPRIHK*PH GWRRPEGVLH*GSSGN*SGQ*DVWLQWLGTLHH AAECGFC*PQQWQVLRGSLCICEGPAEGEGME* TCCPEGSVGGRRRMVHIMKMLVMVLVRASSPRLY LWRRQGRRR*QTG*SEPSGERGAGSLTCVISAL ASEQISIFFGRGNTSTWFKNPSSVEGNSHPCPG SCLPFSHFR*YFFNVSVSGFPCWE*VVESCTLSS PCTQSCVPEAFPLLLRVHRQHPPLRAPQQSSAT HRSFGNALGNCILDKDYLRSLNKLPRQLPLEVD LTKAKRQDLEPSVEEARYNSCRPNMALGHPQLQQ VTSPSRPSHAVIPADQDCSSRSLSSSAVESEAT HQRKLRQKQLQQQFRERMEKQQVRVSTPSAEKS EAAPPAPPVTHSTPVTVSEPLLEKDFLAGVTQEL ISKYLEMLRLLKTTLKSGL*LPMQGMVWSSPRL EQTQPRPLTH*P*TTRW*PRTGLHTAFATRNHK QNLDLGTSKLIALTNAQQETGNLIGRART*RKGN MIHLN*GSGHIIGLCHKGTLENYFLVMKLFIAA GE*TSLRFILLHSEPYQEDLTESPLQLELSTFE KLVHHSSRERLWTDEYLFFGL*GFPLFITELLC* *RWTF*ESPMAPCPQAI*ARLGPKHLPQQLSTF SCFA*RLPSFLYGPCL*CCPH*ALWITTGKRLE QCFSLLCINPGYNLHLI*IIYGFMTLQSCIVSRL TFFPKEGNNHLRPRKKAVFCFFFFFFF*DGVWL CCPDWSSVVQTQLSPQPLGPK*YSHLCLTKYRD YWCEPLCLARKGIFEKANRIPY*QNRIYIYCLSE MLETRIVLHFLNICIHIMRPWGWDPSLNVEFTC VSCICLIHIILCMKQSFCIRRYTAAEEGWVFFS LRVAA*TVVCLVLCDLSHEVTCGIFHFWHHVSAQ KFSDLRAFQLGMLKRNCFYFPISGVRCNPP*P* IGFS*CSDVS*FFPQNNEVCSLTTFFLRGGVWL CSAGWSAAVPS*LIAASACQDSSNSPASGSRVAG ITDTHHDARLIFVFLVEETGFLHVGQAGLKLTS SDLPTSASQSKKY*NYRHEPLRPANLIIFLDVL FWAGLWLILKQ*LPIFWLRPIWEAVNLR*GSLKV LAGSRPFLFLSKLRRQQRSSQELGIQL*IALNS K*CQ*ECEGIFWK*NPTAQQKQVWFI*MVSLNK STKVTN*STKRNLTRSATPRVSAKCTINGC*ETP KV*IFIFIFNSVHC*IM*GKHL*FRKFLMWKKI LKVYY*HKNNLNSSVALVHSSRLVISYTGG*NQ Q*ITPVLTFFVYE*KDVKIIIKPK*QMIMCCMRL HCSSGIKYVSLLGGPFLSYSAPCWFYPYSLHKK YKQRINKSF*SRGGSPCPFPFYLLKTFSSHLHI *GRRPTYLLTKGSRALKE*TGYRSLQPLSVVVVV VVVIPRICH*RG*KVGNIFSFPTKSQEAPQN*E S*LLSLSSEITKILNIAS*DSISGKL*K*LITT HYKEA*H*ITLIGCSYYLRGVLFI*KCEHISCTS RNKGGTIDYDPATIWTS*RK*VGISILS*ND*F RCGKIDRLTFHKM*YPKLRNAWYMCNKRKAIQT VFLRAIFSICDFSLSGSPT*KLAPHPLLFMFSFF *VTSVQA*LK*ERFI*QSLARHHVCFLIL*SLS FTQHTPFF*VVQFCYNTPSSLFPKQNFLFLTTD ANLTSHPTHLTPALDKVVE*LKKILFQFPFGITT AGLLGSHGPTISFHMPFIDLGSGKLMLMAEAIE VQGTKMSSKQHWETEVFKYSNLYNFTFTFIYLI QWKYKGKS*SVSISKGQS*LFLELFKRFIFYS*N YCCPTTFYISQCFHIKTDGKVTCPQGT*FFFFP NC*CCSQSC*YLLLKALMTYHKKELHPSTLVFF TGF*RAKKK*KREG*KKGRRAG*ERAQ*VVFVLW FGFLRE*GIQGMYQKQELHPLEITDGFDFTLSS PASL*TRLS*PSSSLLSIDKQESMV*SCPLTKK LFFPSSPIPITGAGETLHSEGSMQY*KHSPLARN NQLNAALVVSI*ATSHPHPPPQHSISCPQIYSV NVSRSKWSAGWSLGGC*WISANC*C*NSARRQF PQVGTGQVAVVLGHPSHSIERQMG*LESHTAVGG T*TW*SDLER*LMLQRWGQSHPGSRSWRCTVAQ VQRTSLPFQGPENKTTTCQVWCGPWYKMCGANI YF*YNPVRYSRTVFCSIYWLFIFTK*G*K*SS*E GGMSCACVSVCV*LRMTK

Frame 2

RRSRTLPILLCAASI*DLFPLSIA*IELPVCTA RCPRRRSTRIKMSGTEEAILGGRDSHPAAGGGS VLCFGQCQYTAEEYQAIQKALRQRLGPEYISSR MAGGGQKVCYIEGHRVINLANEMFGYNGWAHSIT QQNVDFVDLNNGKFYVGVCAFVRVQLKVRGWSR PAARKDQWVVGEGWFIS*RCWLWC**GPQVQGF IFGEGKEGGGDRRAEASPQVSAGLGV*PASFQLL LQSRFRFFLEEVTHPHGSKIQAAWKVILTPALV PVCRFPTFVSIFSMFLFQASLAGSKW*NPVLSL LRALSHVCRRPSHSYCECTDSTLLSVLLSSPPLR TGVLGMHLETVFWTKTT*DH*ISFHASCLLKWI *LKRRDKILNRLWRRQDTTAADRTWPWDTHSCS R*PPLPDPAML*YRRTRTAAPEA*AHPPWRARPR TSGSSGRSSCSSSSGSGWRSSRFESPRRQLRRV RQRLRPLL*RTALL*LSQNHSWRKTSLQE*LKN *SVNTLKCLDS*RQL*KVGCDSRCRGWCGQALV* SRPSPDL*HISLEQPDGDPEQDSTQRLPPETTS KIWILGPPNL*R*PTHNRKLGIS*EEPGHEEKE I*SILTEAQAT*LDSVTKGLWKTTFWS*NCSSLL ENERHCDLSCFILNLIKRI*LRAHCS*S*ALLK SLSITLVGRGSGQMNTFSSACEASHYLLLNYYV NEDGHFRNHQWLLALKQYRPDLVLSTCLSNCLHS VVLHNVCLLSFTVHAFNVAHIKHCGSRQEKGWS SAFHYFVSIQATIFI*YK*FMDL*HYNPALFQD *HFFLRKEIII*DHEKRLFFVFFFFFFFETGSGC VALTGVQWCKHSSLHNLLGPSDTPTSALQNTGI TGVSHCVWPEKAFLRKQIVYLINKIEYIYIAYL KCLKPELFCIF*IFVYT**DLGDGTQV*TWNSPV FRVYASYT*FCA*NRVFV*EDTLQLKRAGFFFL LGSLHKLLYAWCFATCHTRSRVEFSTSGITSVL RNFLISEHFN*GCSNATVSTSPFQV*DVTHLDHK LAFHSAQMFHNSSPKIMKFAV*QLFF*EGGSGS VAQAGVQRCHLDSLQPPPARIQAILLPQAPE*L GLQTPTTTPD*FLYF**RRRGFSMLARLVSN*PQ VICPPRPPKVKSTRIIGMSHCAQPI**FF*TYY FGLGCG*S*SSDFLFFGSDPFGKL*T*DKEV*K Y*PAADRFYSFRS*GDSKGAPRN*GSSYKLHLTQ NSASENVKESSGNETPQLNKSKFGLFEWFH*IS LQR*QTEAQSAILQEAQHPELVQNVQLTAAEKP PKFEFLFLFLTVYIVK*CKENIYNSESF*CGKRY SKCTINTKII*TVQ*H*FIPLGLLFHTQEDEIN SK*HQF*LFLCMSEKMSKLLLNPNNK**CAA*G STAPLV*SMFHSWVAPFSATQPLVGSILTLSTRN TSRE*INHFKAGVEALALFLSTF*KPSALICIF RAVDQLTYLLREAGPLRNEQGTGASSLFLW*WW WWW*FPEYAIDVGKRLEIFSVSQLRVKKHPKIKS PSFFLCLQR*QRS*T*PVRTVFPESSENN**LP ITRRHSTE*H*LVAVITYGEYYLFENVNILVVL LEIKVAQ*IMILQPFGHHKGNE*E*VFFPRMINS DVER*ID*LSTKCSTPS*EMHGTCATRGKQSKQ FF*EQFSPSVTFHYQVPQLEN*PPTPFSLCSVS SRSRLFKPSSNKKGLYDSHWPGITFVSSFFSLCL SPSTHHSFKLSNFVTTPPPHYSRNRTFCF*PLM PT*LPTLLILHLL*TKLLNDLKKYFFNFPLA*P LLDCWGHMDLRFPSTCPLLIWGLVS*C*WQKQ*K SKALKCPASNTGKQKYSSTVIYTILHSHLFI*Y NGNTKEKVKVSQ*VKDRASFFWNFLNVSYFTLK TTVVQQHFIYHSVSTSKLMAKLHVRRELNFFFFL IANAAVKAANIFCLKH**PIIRKSCTPVPWYFS LGSKEQRKNKKERGKKKGGGQGRREPSRWFLSC GSDS*GSEGFRACTKSKSSTHSKLQMGLILH*VL QLPYEQDFPSPHPHY*V*INRKAWSEAAP*QKS CSFLPLPFQ*LERGRLSILRAQCSIKNIPLWQE ITS*MQH**LVYRLLPTPTHPLSIPSPAPRSIQL MSLGLSGPQVGAWGVADGFLQIADVEILQGGSS HRLELAKWLWCWATRPTPLSAKWGSWKAILLWG AHRLGEVT*RGS*CCRDGGRATQVRGHGDAQLPR CRELPCHSRALKTKPPRVKSGVGHGIKCVALTY TSSIIL*DTAEQSSVVFIGSLYLPSKGENNPHE REGCHVRVCLCVCS*G*RR