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Sequences

Sequence HMGA1.vkAug05

GTTGGAACTAGTCCCTTTGACTCCCCGTTTCTATTTTATTTTCTTCTGGGGCTTTATTTTGCGGGGAGGGGACGGAGGGAGGGGGCTTTCTTCCTCTCCC CCGCGCCCGCGCGCGCCAGGTTTCCTGCCCCAAGGCGGGCTTGGGTGCCCCCTCTGGCAGGAAGTGGGGCCGGGTGCACCCGCGTGGCGGTGCATGCGGT CGGGGTGCGCTGCTTTCCCGCGCCTGCAGGAGCGGCCTGCGCCCCTCCCCCCGCGCGCCCTGGCCCCGGGTCCCGCCGGCCGGCCTGCAACGGCCGGGCG CAGACACTTGCAGAGTCACCCTCGGGCCGGGTCTGGAGCCTGATGCCTCGGGGGTTTAGCCCTAGCCGCTAGGCGGCATCCCAGCCATCACTCTTCCACC TGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGG CAGGCCGCGCAAGCAGCCTCCGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACC CGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGG AGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCC AGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATG GGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACT TAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGT TCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGG CAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCC AACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCC CTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGG TGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGC TCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCA ATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATAC TCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGT GAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTC CCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative donor

Frame 0

VGTSPFDSPFLFYFLLGLYFAGRGRREGAFFLS PAPARARFPAPRRAWVPPLAGSGAGCTRVAVHAV GVRCFPAPAGAACAPPPARPGPGSRRPACNGRA QTLAESPSGRVWSLMPRGFSPSR*AASQPSLFH LLLREGKMSESSSKSSQPLASKQEKDGTEKRGRG RPRKQPPEPSEVPTPKRPRGRPKGSKNKGAAKT RKTTTTPGRKPRGRPKKLEKEEEEGISQESSEE EQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPSP GPPSPPPLAATPIFHLCPHHHTTQHTSRCRAPM G*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSH LAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFTV PSGFP*WGLGGFPWP*KGPKPHLILARPTPLPW QQQVWPMEGGAGPQDSPSQTVFVTTWGSLFILP QLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATAP LPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLR WAGAAPLTLLCFRHSAISPSSDGAPITRSSPCP LPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFVQ CSILRHPSLLLLPAPNVHPHCLLFCPRSPPPRY SLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE *ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWGS LSGFLFAVT*IKKISFSGLE

Frame 1

LELVPLTPRFYFIFFWGFILRGGDGGRGLSSSP PRPRAPGFLPQGGLGCPLWQEVGPGAPAWRCMR SGCAAFPRLQERPAPLPPRALAPGPAGRPATAGR RHLQSHPRAGSGA*CLGGLALAARRHPSHHSST CSLEKGR*VSRARSPASPWPPSRKRTALRSGAG AGRASSLRSPAKCQHLRDLGADQREAKTRVLPRP GKPPQLQEGNQGADPKNWRRRKRRASRRSPRRR SSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLP QAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLPW AEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT *PHPAPAASPLPWWWGHCSLGFWFGGALSAPSL FPLASHSGAWEGSPGLKRGPSPISSWHALLHCPG SSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFP NFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQP PCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*G GLGLLP*PYFASATQPFPPPQMGHQ*QGAHPAR SQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLF NVPFFDIRHCCCYQRQMFILIASCSAHDPLPQDT LCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQS EQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGA PSLVSYLQLLE*KKYPFLDW

Frame 2

WN*SL*LPVSILFSSGALFCGEGTEGGGFLPLP RARARQVSCPKAGLGAPSGRKWGRVHPRGGACG RGALLSRACRSGLRPSPRAPWPRVPPAGLQRPG ADTCRVTLGPGLEPDASGV*P*PLGGIPAITLPP AP*RREDE*VELEVQPALGLQAGKGRH*EAGPG QAAQAASGAQRSANT*ETSGPTKGKQKQGCCQD PENHHNSRKETKGQTQKTGEGGRGGHLAGVLGGG AVTHACRLLLTGGAASFWDWTALLRSHRPHPFP RPTITTASGRHPHLPPVPSPPHYTAHQPLQGSH GLSGEQFSPGLSSQLPPPTHAYTHALLDKANIPL SRTLHLLRPHSLGGGDIALWAFGLGAPSLLLHC SLWLPIVGPGRVPLALKGAQAPSHPGTPYSTAL AAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPSP TSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSP LALRLGSEYILW*WRCSHLLSR*GPRALWPPPE VGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLPA PNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCS MFHSSTSVIAAATSAKCSSSLPPVLPTIPSPKI LFVGKRGWGMAGWVTDYPSPREGGALPLGCCSRV SKGAQIDHKGCRGHLLPLFCWGGVAMICPSLGL PLWFPICSYLNKKNILFWTG

Sequence HMGA1.veAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGAGCCCAGCGAAGTGCCAACACCT AAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCA AAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCT TCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCAC CTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCC CCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGG GACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCT TAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATT CCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTAC AGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGAT GCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAG CCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTC CATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCA AATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGA CTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTC CTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCC TTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPEPSEVPTP KRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRP KKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQL PSGTGQLCSAPTAPTPSPGPPSPPPLAATPIFH LCPHHHTTQHTSRCRAPMG*VGSSFPLASVPSS PRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVVG TLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPWP *KGPKPHLILARPTPLPWQQQVWPMEGGAGPQD SPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSVT GRQEG*VPYSLFTVATAPLPSAWDLSTYCGDGD AVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRHS AISPSSDGAPITRSSPCPLPTPLLLLPAPQGSGS IFPLFTNYLWTVVLFFVQCSILRHPSLLLLPAP NVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*P TTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGATS SPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKIS FSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLRSPAKCQHL RDLGADQREAKTRVLPRPGKPPQLQEGNQGADP KNWRRRKRRASRRSPRRRSSDPCVPPAPHWRSS FLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSST CALTTTLHSTPAAAGLPWAEWGAVFPWPQFPAP PAHPRIHTCPPGQG*HPT*PHPAPAASPLPWWW GHCSLGFWFGGALSAPSLFPLASHSGAWEGSPGL KRGPSPISSWHALLHCPGSSRCGQWRGVLAPRI PPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLRL QEGRRGESPTPSSLWPQPPCPPPGI*VHIVVMEM QSLIVQVRPKSPVAAT*GGLGLLP*PYFASATQ PFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVLV PFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQRQ MFILIASCSAHDPLPQDTLCGEEGLGHGRLGDR LPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GPP PPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKYP FLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASGAQRSANT *ETSGPTKGKQKQGCCQDPENHHNSRKETKGQTQ KTGEGGRGGHLAGVLGGGAVTHACRLLLTGGAA SFWDWTALLRSHRPHPFPRPTITTASGRHPHLP PVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQLP PPTHAYTHALLDKANIPLSRTLHLLRPHSLGGG DIALWAFGLGAPSLLLHCSLWLPIVGPGRVPLA LKGAQAPSHPGTPYSTALAAAGVANGGGCWPPGF PQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFGY RKAGGVSPLLPLHCGHSPLALRLGSEYILW*WR CSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPLS HFPLLRWGTNNKELTLPAPNPPPAPPCPPRFWF HFSSVHKLPLDSCVVFCSMFHSSTSVIAAATSA KCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVTD YPSPREGGALPLGCCSRVSKGAQIDHKGCRGHL LPLFCWGGVAMICPSLGLPLWFPICSYLNKKNI LFWTG

Sequence HMGA1.lAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGAGCCCAGCG AAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACC AAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCA CTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCA CCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTC AGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACT CCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAG GGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGC TGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAG CCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATT GTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTG CTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCC CAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGC TGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCA GGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGT GTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAA TAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPEPS EVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRKP RGRPKKLEKEEEEGISQESSEEEQ*PMRAACSS LEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLAA TPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPLAS VPSSPRPPTHTHMPSWTRLTSHLAAPCTCCVPT PLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGLG GFPWP*KGPKPHLILARPTPLPWQQQVWPMEGGA GPQDSPSQTVFVTTWGSLFILPQLP*SPY*VGQ PPSVTGRQEG*VPYSLFTVATAPLPSAWDLSTY CGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTLLC FRHSAISPSSDGAPITRSSPCPLPTPLLLLPAP QGSGSIFPLFTNYLWTVVLFFVQCSILRHPSLL LLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGAWQ AG*PTTPVPGKVGPCP*DAAAE*ARGPKSTIKG VGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT* IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLRSPA KCQHLRDLGADQREAKTRVLPRPGKPPQLQEGN QGADPKNWRRRKRRASRRSPRRRSSDPCVPPAPH WRSSFLLGLDSFAPLPPPPPLPQAHHHHRLWPP PPSSTCALTTTLHSTPAAAGLPWAEWGAVFPWP QFPAPPAHPRIHTCPPGQG*HPT*PHPAPAASPL PWWWGHCSLGFWFGGALSAPSLFPLASHSGAWE GSPGLKRGPSPISSWHALLHCPGSSRCGQWRGV LAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRLDS PLRLQEGRRGESPTPSSLWPQPPCPPPGI*VHI VVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PYF ASATQPFPPPQMGHQ*QGAHPARSQPPSCSSLPP KVLVPFFLCSQTTSGQLCCFLFNVPFFDIRHCC CYQRQMFILIASCSAHDPLPQDTLCGEEGLGHG RLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV *GPPPPPVLLGRGSHDLSQPGAPSLVSYLQLLE *KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGAQR SANT*ETSGPTKGKQKQGCCQDPENHHNSRKET KGQTQKTGEGGRGGHLAGVLGGGAVTHACRLLL TGGAASFWDWTALLRSHRPHPFPRPTITTASGRH PHLPPVPSPPHYTAHQPLQGSHGLSGEQFSPGL SSQLPPPTHAYTHALLDKANIPLSRTLHLLRPH SLGGGDIALWAFGLGAPSLLLHCSLWLPIVGPGR VPLALKGAQAPSHPGTPYSTALAAAGVANGGGC WPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGWT APFGYRKAGGVSPLLPLHCGHSPLALRLGSEYIL W*WRCSHLLSR*GPRALWPPPEVGWGCSPNPTL LPPLSHFPLLRWGTNNKELTLPAPNPPPAPPCP PRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVIAA ATSAKCSSSLPPVLPTIPSPKILFVGKRGWGMA GWVTDYPSPREGGALPLGCCSRVSKGAQIDHKG CRGHLLPLFCWGGVAMICPSLGLPLWFPICSYLN KKNILFWTG

Sequence HMGA1.uAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA GGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCA CAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCAT GCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCA CCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGA GCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTG CACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTC CCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGT GGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTC CCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCC TGGGATCTGAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGC CACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTT CTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACC AGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGG TGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGG CCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAA AATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV GSQEPSEVPTPKRPRGRPKGSKNKGAAKTRKTT TTPGRKPRGRPKKLEKEEEEGISQESSEEEQ*PM RAACSSLEEQLPSGTGQLCSAPTAPTPSPGPPS PPPLAATPIFHLCPHHHTTQHTSRCRAPMG*VG SSFPLASVPSSPRPPTHTHMPSWTRLTSHLAAPC TCCVPTPLVVGTLLSGLLVWGRPLCSFTVPSGF P*WGLGGFPWP*KGPKPHLILARPTPLPWQQQV WPMEGGAGPQDSPSQTVFVTTWGSLFILPQLP*S PY*VGQPPSVTGRQEG*VPYSLFTVATAPLPSA WDLSHLLSR*GPRALWPPPEVGWGCSPNPTLLP PLSHFPLLRWGTNNKELTLPAPNPPPAPPCPPRF WFHFSSVHKLPLDSCVVFCSMFHSSTSVIAAAT SAKCSSSLPPVLPTIPSPKILFVGKRGWGMAGW VTDYPSPREGGALPLGCCSRVSKGAQIDHKGCRG HLLPLFCWGGVAMICPSLGLPLWFPICSYLNKK NILFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW* GVRSPAKCQHLRDLGADQREAKTRVLPRPGKPP QLQEGNQGADPKNWRRRKRRASRRSPRRRSSDP CVPPAPHWRSSFLLGLDSFAPLPPPPPLPQAHHH HRLWPPPPSSTCALTTTLHSTPAAAGLPWAEWG AVFPWPQFPAPPAHPRIHTCPPGQG*HPT*PHP APAASPLPWWWGHCSLGFWFGGALSAPSLFPLAS HSGAWEGSPGLKRGPSPISSWHALLHCPGSSRC GQWRGVLAPRIPPAKLSLSPRGAHFSSFPNFPS PRTRLDSPLRLQEGRRGESPTPSSLWPQPPCPPP GI*VTYCPGEAQEPCGRHLRWAGAAPLTLLCFR HSAISPSSDGAPITRSSPCPLPTPLLLLPAPQG SGSIFPLFTNYLWTVVLFFVQCSILRHPSLLLLP APNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG *PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVG ATSSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKK ISFSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG RESGAQRSANT*ETSGPTKGKQKQGCCQDPENHH NSRKETKGQTQKTGEGGRGGHLAGVLGGGAVTH ACRLLLTGGAASFWDWTALLRSHRPHPFPRPTI TTASGRHPHLPPVPSPPHYTAHQPLQGSHGLSGE QFSPGLSSQLPPPTHAYTHALLDKANIPLSRTL HLLRPHSLGGGDIALWAFGLGAPSLLLHCSLWL PIVGPGRVPLALKGAQAPSHPGTPYSTALAAAGV ANGGGCWPPGFPQPNCLCHHVGLTFHPSPTSLV PVLGWTAPFGYRKAGGVSPLLPLHCGHSPLALR LGSESLIVQVRPKSPVAAT*GGLGLLP*PYFASA TQPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKV LVPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCY QRQMFILIASCSAHDPLPQDTLCGEEGLGHGRLG DRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*G PPPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*K KYPFLDW

Sequence HMGA1.gAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG GGACAGCGCTGGTAGGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGAC CCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAG GAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCC CAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCAT GGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCAC TTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTG TTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTG GCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCC CAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCC CCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAG GTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCG CTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTC AATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATA CTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAG TGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCT CCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP GTALVGSQEPSEVPTPKRPRGRPKGSKNKGAAKT RKTTTTPGRKPRGRPKKLEKEEEEGISQESSEE EQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAPM G*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSH LAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLPW QQQVWPMEGGAGPQDSPSQTVFVTTWGSLFILP QLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLR WAGAAPLTLLCFRHSAISPSSDGAPITRSSPCP LPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPRY SLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE *ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG SLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP GQRW*GVRSPAKCQHLRDLGADQREAKTRVLPR PGKPPQLQEGNQGADPKNWRRRKRRASRRSPRRR SSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLP QAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT *PHPAPAASPLPWWWGHCSLGFWFGGALSAPSL FPLASHSGAWEGSPGLKRGPSPISSWHALLHCP GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFP NFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQP PCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT* GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPAR SQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLF NVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQS EQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGA PSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESGAQRSANT*ETSGPTKGKQKQGCCQD PENHHNSRKETKGQTQKTGEGGRGGHLAGVLGG GAVTHACRLLLTGGAASFWDWTALLRSHRPHPFP RPTITTASGRHPHLPPVPSPPHYTAHQPLQGSH GLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLHC SLWLPIVGPGRVPLALKGAQAPSHPGTPYSTAL AAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSP LALRLGSEYILW*WRCSHLLSR*GPRALWPPPE VGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCS MFHSSTSVIAAATSAKCSSSLPPVLPTIPSPKI LFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLGL PLWFPICSYLNKKNILFWTG

Sequence HMGA1.dAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGGAGCC CAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGG AAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCT CCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGC CGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTG GCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCC CCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCT GGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGG GGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTG GACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTAC ATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTA CTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTG CCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATT GCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCA TGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAA AGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTAC TTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative donor

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL QAGKGRH*EAGPGQAAQAASGESRDSAGRESGA QRSANT*ETSGPTKGKQKQGCCQDPENHHNSRK ETKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL LTGGAASFWDWTALLRSHRPHPFPRPTITTASG RHPHLPPVPSPPHYTAHQPLQGSHGLSGEQFSP GLSSQLPPPTHAYTHALLDKANIPLSRTLHLLRP HSLGGGDIALWAFGLGAPSLLLHCSLWLPIVGP GRVPLALKGAQAPSHPGTPYSTALAAAGVANGG GCWPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGW TAPFGYRKAGGVSPLLPLHCGHSPLALRLGSEY ILW*WRCSHLLSR*GPRALWPPPEVGWGCSPNP TLLPPLSHFPLLRWGTNNKELTLPAPNPPPAPPC PPRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVI AAATSAKCSSSLPPVLPTIPSPKILFVGKRGWG MAGWVTDYPSPREGGALPLGCCSRVSKGAQIDHK GCRGHLLPLFCWGGVAMICPSLGLPLWFPICSY LNKKNILFWTG

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA GGRARASQPSLFHLLLREGKMSESSSKSSQPLA SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQEP SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGR KPRGRPKKLEKEEEEGISQESSEEEQ*PMRAAC SSLEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLA ATPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPL ASVPSSPRPPTHTHMPSWTRLTSHLAAPCTCCV PTPLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGL GGFPWP*KGPKPHLILARPTPLPWQQQVWPMEG GAGPQDSPSQTVFVTTWGSLFILPQLP*SPY*V GQPPSVTGRQEG*VPYSLFTVATAPLPSAWDLST YCGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTL LCFRHSAISPSSDGAPITRSSPCPLPTPLLLLP APQGSGSIFPLFTNYLWTVVLFFVQCSILRHPSL LLLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGA WQAG*PTTPVPGKVGPCP*DAAAE*ARGPKSTI KGVGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT *IKKISFSGLE

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRS PAKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPA PHWRSSFLLGLDSFAPLPPPPPLPQAHHHHRLW PPPPSSTCALTTTLHSTPAAAGLPWAEWGAVFPW PQFPAPPAHPRIHTCPPGQG*HPT*PHPAPAAS PLPWWWGHCSLGFWFGGALSAPSLFPLASHSGA WEGSPGLKRGPSPISSWHALLHCPGSSRCGQWRG VLAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRL DSPLRLQEGRRGESPTPSSLWPQPPCPPPGI*V HIVVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PY FASATQPFPPPQMGHQ*QGAHPARSQPPSCSSL PPKVLVPFFLCSQTTSGQLCCFLFNVPFFDIRH CCCYQRQMFILIASCSAHDPLPQDTLCGEEGLGH GRLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP* RV*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQL LE*KKYPFLDW

Sequence HMGA1.rAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGGAGCC CAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCG GAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCC CTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGC TCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACA TCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCT TCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACT GCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATC CTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCA CAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCA CCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCC TGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTT TTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCC AAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCA GCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCT GGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative donor

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL QAGKGRH*EAGPGQAAQAASGESRDSAGRESGA QRSANT*ETSGPTKGKQKQG*EGGRGGHLAGVL GGGAVTHACRLLLTGGAASFWDWTALLRSHRPHP FPRPTITTASGRHPHLPPVPSPPHYTAHQPLQG SHGLSGEQFSPGLSSQLPPPTHAYTHALLDKAN IPLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLL HCSLWLPIVGPGRVPLALKGAQAPSHPGTPYST ALAAAGVANGGGCWPPGFPQPNCLCHHVGLTFH PSPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGH SPLALRLGSEYILW*WRCSHLLSR*GPRALWPP PEVGWGCSPNPTLLPPLSHFPLLRWGTNNKELT LPAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVF CSMFHSSTSVIAAATSAKCSSSLPPVLPTIPSP KILFVGKRGWGMAGWVTDYPSPREGGALPLGCC SRVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSL GLPLWFPICSYLNKKNILFWTG

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA GGRARASQPSLFHLLLREGKMSESSSKSSQPLA SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQEP SEVPTPKRPRGRPKGSKNKGEKEEEEGISQESS EEEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPT PSPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRA PMG*VGSSFPLASVPSSPRPPTHTHMPSWTRLT SHLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCS FTVPSGFP*WGLGGFPWP*KGPKPHLILARPTPL PWQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFI LPQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVA TAPLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRH LRWAGAAPLTLLCFRHSAISPSSDGAPITRSSP CPLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLF FVQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPP RYSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAA AE*ARGPKSTIKGVGATSSPCSVGEG*P*FVPA WGSLSGFLFAVT*IKKISFSGLE

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRS PAKCQHLRDLGADQREAKTRVRRRKRRASRRSPR RRSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPP LPQAHHHHRLWPPPPSSTCALTTTLHSTPAAAG LPWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*H PT*PHPAPAASPLPWWWGHCSLGFWFGGALSAP SLFPLASHSGAWEGSPGLKRGPSPISSWHALLH CPGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSS FPNFPSPRTRLDSPLRLQEGRRGESPTPSSLWP QPPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAA T*GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHP ARSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCF LFNVPFFDIRHCCCYQRQMFILIASCSAHDPLP QDTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQ QSEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQP GAPSLVSYLQLLE*KKYPFLDW

Sequence HMGA1.oAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG GGACAGCGCTGGTAGGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGA AGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAG CTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACA CTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATAC ACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTT TTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCC ATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTT TGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGA GTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAG GTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGA TGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACA AACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCC TCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAA GGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGG GAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative donor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP GTALVGSQEPSEVPTPKRPRGRPKGSKNKGEKEE EEGISQESSEEEQ*PMRAACSSLEEQLPSGTGQ LCSAPTAPTPSPGPPSPPPLAATPIFHLCPHHH TTQHTSRCRAPMG*VGSSFPLASVPSSPRPPTHT HMPSWTRLTSHLAAPCTCCVPTPLVVGTLLSGL LVWGRPLCSFTVPSGFP*WGLGGFPWP*KGPKP HLILARPTPLPWQQQVWPMEGGAGPQDSPSQTVF VTTWGSLFILPQLP*SPY*VGQPPSVTGRQEG* VPYSLFTVATAPLPSAWDLSTYCGDGDAVTYCP GEAQEPCGRHLRWAGAAPLTLLCFRHSAISPSSD GAPITRSSPCPLPTPLLLLPAPQGSGSIFPLFT NYLWTVVLFFVQCSILRHPSLLLLPAPNVHPHC LLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVPGK VGPCP*DAAAE*ARGPKSTIKGVGATSSPCSVG EG*P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP GQRW*GVRSPAKCQHLRDLGADQREAKTRVRRR KRRASRRSPRRRSSDPCVPPAPHWRSSFLLGLDS FAPLPPPPPLPQAHHHHRLWPPPPSSTCALTTT LHSTPAAAGLPWAEWGAVFPWPQFPAPPAHPRI HTCPPGQG*HPT*PHPAPAASPLPWWWGHCSLGF WFGGALSAPSLFPLASHSGAWEGSPGLKRGPSP ISSWHALLHCPGSSRCGQWRGVLAPRIPPAKLS LSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRRGE SPTPSSLWPQPPCPPPGI*VHIVVMEMQSLIVQ VRPKSPVAAT*GGLGLLP*PYFASATQPFPPPQ MGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLCSQ TTSGQLCCFLFNVPFFDIRHCCCYQRQMFILIA SCSAHDPLPQDTLCGEEGLGHGRLGDRLPQSQG RWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVLLG RGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESGAQRSANT*ETSGPTKGKQKQG*EGG RGGHLAGVLGGGAVTHACRLLLTGGAASFWDWT ALLRSHRPHPFPRPTITTASGRHPHLPPVPSPPH YTAHQPLQGSHGLSGEQFSPGLSSQLPPPTHAY THALLDKANIPLSRTLHLLRPHSLGGGDIALWA FGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQAP SHPGTPYSTALAAAGVANGGGCWPPGFPQPNCL CHHVGLTFHPSPTSLVPVLGWTAPFGYRKAGGV SPLLPLHCGHSPLALRLGSEYILW*WRCSHLLSR *GPRALWPPPEVGWGCSPNPTLLPPLSHFPLLR WGTNNKELTLPAPNPPPAPPCPPRFWFHFSSVH KLPLDSCVVFCSMFHSSTSVIAAATSAKCSSSLP PVLPTIPSPKILFVGKRGWGMAGWVTDYPSPRE GGALPLGCCSRVSKGAQIDHKGCRGHLLPLFCW GGVAMICPSLGLPLWFPICSYLNKKNILFWTG