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Sequences

Sequence HMGA1.uAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA GGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCA CAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCAT GCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCA CCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGA GCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTG CACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTC CCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGT GGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTC CCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCC TGGGATCTGAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGC CACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTT CTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACC AGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGG TGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGG CCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAA AATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV GSQEPSEVPTPKRPRGRPKGSKNKGAAKTRKTT TTPGRKPRGRPKKLEKEEEEGISQESSEEEQ*PM RAACSSLEEQLPSGTGQLCSAPTAPTPSPGPPS PPPLAATPIFHLCPHHHTTQHTSRCRAPMG*VG SSFPLASVPSSPRPPTHTHMPSWTRLTSHLAAPC TCCVPTPLVVGTLLSGLLVWGRPLCSFTVPSGF P*WGLGGFPWP*KGPKPHLILARPTPLPWQQQV WPMEGGAGPQDSPSQTVFVTTWGSLFILPQLP*S PY*VGQPPSVTGRQEG*VPYSLFTVATAPLPSA WDLSHLLSR*GPRALWPPPEVGWGCSPNPTLLP PLSHFPLLRWGTNNKELTLPAPNPPPAPPCPPRF WFHFSSVHKLPLDSCVVFCSMFHSSTSVIAAAT SAKCSSSLPPVLPTIPSPKILFVGKRGWGMAGW VTDYPSPREGGALPLGCCSRVSKGAQIDHKGCRG HLLPLFCWGGVAMICPSLGLPLWFPICSYLNKK NILFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW* GVRSPAKCQHLRDLGADQREAKTRVLPRPGKPP QLQEGNQGADPKNWRRRKRRASRRSPRRRSSDP CVPPAPHWRSSFLLGLDSFAPLPPPPPLPQAHHH HRLWPPPPSSTCALTTTLHSTPAAAGLPWAEWG AVFPWPQFPAPPAHPRIHTCPPGQG*HPT*PHP APAASPLPWWWGHCSLGFWFGGALSAPSLFPLAS HSGAWEGSPGLKRGPSPISSWHALLHCPGSSRC GQWRGVLAPRIPPAKLSLSPRGAHFSSFPNFPS PRTRLDSPLRLQEGRRGESPTPSSLWPQPPCPPP GI*VTYCPGEAQEPCGRHLRWAGAAPLTLLCFR HSAISPSSDGAPITRSSPCPLPTPLLLLPAPQG SGSIFPLFTNYLWTVVLFFVQCSILRHPSLLLLP APNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG *PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVG ATSSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKK ISFSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG RESGAQRSANT*ETSGPTKGKQKQGCCQDPENHH NSRKETKGQTQKTGEGGRGGHLAGVLGGGAVTH ACRLLLTGGAASFWDWTALLRSHRPHPFPRPTI TTASGRHPHLPPVPSPPHYTAHQPLQGSHGLSGE QFSPGLSSQLPPPTHAYTHALLDKANIPLSRTL HLLRPHSLGGGDIALWAFGLGAPSLLLHCSLWL PIVGPGRVPLALKGAQAPSHPGTPYSTALAAAGV ANGGGCWPPGFPQPNCLCHHVGLTFHPSPTSLV PVLGWTAPFGYRKAGGVSPLLPLHCGHSPLALR LGSESLIVQVRPKSPVAAT*GGLGLLP*PYFASA TQPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKV LVPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCY QRQMFILIASCSAHDPLPQDTLCGEEGLGHGRLG DRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*G PPPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*K KYPFLDW

Sequence HMGA1.gAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG GGACAGCGCTGGTAGGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGAC CCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAG GAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCC CAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCAT GGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCAC TTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTG TTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTG GCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCC CAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCC CCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAG GTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCG CTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTC AATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATA CTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAG TGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCT CCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP GTALVGSQEPSEVPTPKRPRGRPKGSKNKGAAKT RKTTTTPGRKPRGRPKKLEKEEEEGISQESSEE EQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAPM G*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSH LAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLPW QQQVWPMEGGAGPQDSPSQTVFVTTWGSLFILP QLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLR WAGAAPLTLLCFRHSAISPSSDGAPITRSSPCP LPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPRY SLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE *ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG SLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP GQRW*GVRSPAKCQHLRDLGADQREAKTRVLPR PGKPPQLQEGNQGADPKNWRRRKRRASRRSPRRR SSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLP QAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT *PHPAPAASPLPWWWGHCSLGFWFGGALSAPSL FPLASHSGAWEGSPGLKRGPSPISSWHALLHCP GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFP NFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQP PCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT* GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPAR SQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLF NVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQS EQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGA PSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESGAQRSANT*ETSGPTKGKQKQGCCQD PENHHNSRKETKGQTQKTGEGGRGGHLAGVLGG GAVTHACRLLLTGGAASFWDWTALLRSHRPHPFP RPTITTASGRHPHLPPVPSPPHYTAHQPLQGSH GLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLHC SLWLPIVGPGRVPLALKGAQAPSHPGTPYSTAL AAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSP LALRLGSEYILW*WRCSHLLSR*GPRALWPPPE VGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCS MFHSSTSVIAAATSAKCSSSLPPVLPTIPSPKI LFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLGL PLWFPICSYLNKKNILFWTG

Sequence HMGA1.dAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGGAGCC CAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGG AAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCT CCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGC CGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTG GCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCC CCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCT GGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGG GGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTG GACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTAC ATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTA CTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTG CCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATT GCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCA TGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAA AGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTAC TTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL QAGKGRH*EAGPGQAAQAASGESRDSAGRESGA QRSANT*ETSGPTKGKQKQGCCQDPENHHNSRK ETKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL LTGGAASFWDWTALLRSHRPHPFPRPTITTASG RHPHLPPVPSPPHYTAHQPLQGSHGLSGEQFSP GLSSQLPPPTHAYTHALLDKANIPLSRTLHLLRP HSLGGGDIALWAFGLGAPSLLLHCSLWLPIVGP GRVPLALKGAQAPSHPGTPYSTALAAAGVANGG GCWPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGW TAPFGYRKAGGVSPLLPLHCGHSPLALRLGSEY ILW*WRCSHLLSR*GPRALWPPPEVGWGCSPNP TLLPPLSHFPLLRWGTNNKELTLPAPNPPPAPPC PPRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVI AAATSAKCSSSLPPVLPTIPSPKILFVGKRGWG MAGWVTDYPSPREGGALPLGCCSRVSKGAQIDHK GCRGHLLPLFCWGGVAMICPSLGLPLWFPICSY LNKKNILFWTG

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA GGRARASQPSLFHLLLREGKMSESSSKSSQPLA SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQEP SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGR KPRGRPKKLEKEEEEGISQESSEEEQ*PMRAAC SSLEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLA ATPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPL ASVPSSPRPPTHTHMPSWTRLTSHLAAPCTCCV PTPLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGL GGFPWP*KGPKPHLILARPTPLPWQQQVWPMEG GAGPQDSPSQTVFVTTWGSLFILPQLP*SPY*V GQPPSVTGRQEG*VPYSLFTVATAPLPSAWDLST YCGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTL LCFRHSAISPSSDGAPITRSSPCPLPTPLLLLP APQGSGSIFPLFTNYLWTVVLFFVQCSILRHPSL LLLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGA WQAG*PTTPVPGKVGPCP*DAAAE*ARGPKSTI KGVGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT *IKKISFSGLE

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRS PAKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPA PHWRSSFLLGLDSFAPLPPPPPLPQAHHHHRLW PPPPSSTCALTTTLHSTPAAAGLPWAEWGAVFPW PQFPAPPAHPRIHTCPPGQG*HPT*PHPAPAAS PLPWWWGHCSLGFWFGGALSAPSLFPLASHSGA WEGSPGLKRGPSPISSWHALLHCPGSSRCGQWRG VLAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRL DSPLRLQEGRRGESPTPSSLWPQPPCPPPGI*V HIVVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PY FASATQPFPPPQMGHQ*QGAHPARSQPPSCSSL PPKVLVPFFLCSQTTSGQLCCFLFNVPFFDIRH CCCYQRQMFILIASCSAHDPLPQDTLCGEEGLGH GRLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP* RV*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQL LE*KKYPFLDW

Sequence HMGA1.rAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGGAGCC CAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCG GAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCC CTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGC TCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACA TCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCT TCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACT GCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATC CTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCA CAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCA CCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCC TGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTT TTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCC AAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCA GCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCT GGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL QAGKGRH*EAGPGQAAQAASGESRDSAGRESGA QRSANT*ETSGPTKGKQKQG*EGGRGGHLAGVL GGGAVTHACRLLLTGGAASFWDWTALLRSHRPHP FPRPTITTASGRHPHLPPVPSPPHYTAHQPLQG SHGLSGEQFSPGLSSQLPPPTHAYTHALLDKAN IPLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLL HCSLWLPIVGPGRVPLALKGAQAPSHPGTPYST ALAAAGVANGGGCWPPGFPQPNCLCHHVGLTFH PSPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGH SPLALRLGSEYILW*WRCSHLLSR*GPRALWPP PEVGWGCSPNPTLLPPLSHFPLLRWGTNNKELT LPAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVF CSMFHSSTSVIAAATSAKCSSSLPPVLPTIPSP KILFVGKRGWGMAGWVTDYPSPREGGALPLGCC SRVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSL GLPLWFPICSYLNKKNILFWTG

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA GGRARASQPSLFHLLLREGKMSESSSKSSQPLA SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQEP SEVPTPKRPRGRPKGSKNKGEKEEEEGISQESS EEEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPT PSPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRA PMG*VGSSFPLASVPSSPRPPTHTHMPSWTRLT SHLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCS FTVPSGFP*WGLGGFPWP*KGPKPHLILARPTPL PWQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFI LPQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVA TAPLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRH LRWAGAAPLTLLCFRHSAISPSSDGAPITRSSP CPLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLF FVQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPP RYSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAA AE*ARGPKSTIKGVGATSSPCSVGEG*P*FVPA WGSLSGFLFAVT*IKKISFSGLE

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRS PAKCQHLRDLGADQREAKTRVRRRKRRASRRSPR RRSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPP LPQAHHHHRLWPPPPSSTCALTTTLHSTPAAAG LPWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*H PT*PHPAPAASPLPWWWGHCSLGFWFGGALSAP SLFPLASHSGAWEGSPGLKRGPSPISSWHALLH CPGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSS FPNFPSPRTRLDSPLRLQEGRRGESPTPSSLWP QPPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAA T*GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHP ARSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCF LFNVPFFDIRHCCCYQRQMFILIASCSAHDPLP QDTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQ QSEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQP GAPSLVSYLQLLE*KKYPFLDW

Sequence HMGA1.oAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG GGACAGCGCTGGTAGGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGA AGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAG CTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACA CTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATAC ACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTT TTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCC ATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTT TGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGA GTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAG GTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGA TGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACA AACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCC TCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAA GGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGG GAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP GTALVGSQEPSEVPTPKRPRGRPKGSKNKGEKEE EEGISQESSEEEQ*PMRAACSSLEEQLPSGTGQ LCSAPTAPTPSPGPPSPPPLAATPIFHLCPHHH TTQHTSRCRAPMG*VGSSFPLASVPSSPRPPTHT HMPSWTRLTSHLAAPCTCCVPTPLVVGTLLSGL LVWGRPLCSFTVPSGFP*WGLGGFPWP*KGPKP HLILARPTPLPWQQQVWPMEGGAGPQDSPSQTVF VTTWGSLFILPQLP*SPY*VGQPPSVTGRQEG* VPYSLFTVATAPLPSAWDLSTYCGDGDAVTYCP GEAQEPCGRHLRWAGAAPLTLLCFRHSAISPSSD GAPITRSSPCPLPTPLLLLPAPQGSGSIFPLFT NYLWTVVLFFVQCSILRHPSLLLLPAPNVHPHC LLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVPGK VGPCP*DAAAE*ARGPKSTIKGVGATSSPCSVG EG*P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP GQRW*GVRSPAKCQHLRDLGADQREAKTRVRRR KRRASRRSPRRRSSDPCVPPAPHWRSSFLLGLDS FAPLPPPPPLPQAHHHHRLWPPPPSSTCALTTT LHSTPAAAGLPWAEWGAVFPWPQFPAPPAHPRI HTCPPGQG*HPT*PHPAPAASPLPWWWGHCSLGF WFGGALSAPSLFPLASHSGAWEGSPGLKRGPSP ISSWHALLHCPGSSRCGQWRGVLAPRIPPAKLS LSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRRGE SPTPSSLWPQPPCPPPGI*VHIVVMEMQSLIVQ VRPKSPVAAT*GGLGLLP*PYFASATQPFPPPQ MGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLCSQ TTSGQLCCFLFNVPFFDIRHCCCYQRQMFILIA SCSAHDPLPQDTLCGEEGLGHGRLGDRLPQSQG RWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVLLG RGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESGAQRSANT*ETSGPTKGKQKQG*EGG RGGHLAGVLGGGAVTHACRLLLTGGAASFWDWT ALLRSHRPHPFPRPTITTASGRHPHLPPVPSPPH YTAHQPLQGSHGLSGEQFSPGLSSQLPPPTHAY THALLDKANIPLSRTLHLLRPHSLGGGDIALWA FGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQAP SHPGTPYSTALAAAGVANGGGCWPPGFPQPNCL CHHVGLTFHPSPTSLVPVLGWTAPFGYRKAGGV SPLLPLHCGHSPLALRLGSEYILW*WRCSHLLSR *GPRALWPPPEVGWGCSPNPTLLPPLSHFPLLR WGTNNKELTLPAPNPPPAPPCPPRFWFHFSSVH KLPLDSCVVFCSMFHSSTSVIAAATSAKCSSSLP PVLPTIPSPKILFVGKRGWGMAGWVTDYPSPRE GGALPLGCCSRVSKGAQIDHKGCRGHLLPLFCW GGVAMICPSLGLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.vmAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA GGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCA TCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGC TCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCAC ACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCT CCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGG GCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTG GCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGT GGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTC CCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAA GAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAA TAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTG GACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGC CCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCT GCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCC ATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV GSQKEPSEVPTPKRPRGRPKGSKNKGEKEEEEG ISQESSEEEQ*PMRAACSSLEEQLPSGTGQLCSA PTAPTPSPGPPSPPPLAATPIFHLCPHHHTTQH TSRCRAPMG*VGSSFPLASVPSSPRPPTHTHMP SWTRLTSHLAAPCTCCVPTPLVVGTLLSGLLVWG RPLCSFTVPSGFP*WGLGGFPWP*KGPKPHLIL ARPTPLPWQQQVWPMEGGAGPQDSPSQTVFVTT WGSLFILPQLP*SPY*VGQPPSVTGRQEG*VPYS LFTVATAPLPSAWDLSTYCGDGDAVTYCPGEAQ EPCGRHLRWAGAAPLTLLCFRHSAISPSSDGAP ITRSSPCPLPTPLLLLPAPQGSGSIFPLFTNYLW TVVLFFVQCSILRHPSLLLLPAPNVHPHCLLFC PRSPPPRYSLWGRGAGAWQAG*PTTPVPGKVGP CP*DAAAE*ARGPKSTIKGVGATSSPCSVGEG*P *FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW* GVRRSPAKCQHLRDLGADQREAKTRVRRRKRRA SRRSPRRRSSDPCVPPAPHWRSSFLLGLDSFAP LPPPPPLPQAHHHHRLWPPPPSSTCALTTTLHST PAAAGLPWAEWGAVFPWPQFPAPPAHPRIHTCP PGQG*HPT*PHPAPAASPLPWWWGHCSLGFWFG GALSAPSLFPLASHSGAWEGSPGLKRGPSPISSW HALLHCPGSSRCGQWRGVLAPRIPPAKLSLSPR GAHFSSFPNFPSPRTRLDSPLRLQEGRRGESPT PSSLWPQPPCPPPGI*VHIVVMEMQSLIVQVRPK SPVAAT*GGLGLLP*PYFASATQPFPPPQMGHQ *QGAHPARSQPPSCSSLPPKVLVPFFLCSQTTS GQLCCFLFNVPFFDIRHCCCYQRQMFILIASCSA HDPLPQDTLCGEEGLGHGRLGDRLPQSQGRWGP APRMLQQSEQGGPNRP*RV*GPPPPPVLLGRGS HDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG RESEGAQRSANT*ETSGPTKGKQKQG*EGGRGGH LAGVLGGGAVTHACRLLLTGGAASFWDWTALLR SHRPHPFPRPTITTASGRHPHLPPVPSPPHYTA HQPLQGSHGLSGEQFSPGLSSQLPPPTHAYTHAL LDKANIPLSRTLHLLRPHSLGGGDIALWAFGLG APSLLLHCSLWLPIVGPGRVPLALKGAQAPSHP GTPYSTALAAAGVANGGGCWPPGFPQPNCLCHHV GLTFHPSPTSLVPVLGWTAPFGYRKAGGVSPLL PLHCGHSPLALRLGSEYILW*WRCSHLLSR*GP RALWPPPEVGWGCSPNPTLLPPLSHFPLLRWGTN NKELTLPAPNPPPAPPCPPRFWFHFSSVHKLPL DSCVVFCSMFHSSTSVIAAATSAKCSSSLPPVL PTIPSPKILFVGKRGWGMAGWVTDYPSPREGGAL PLGCCSRVSKGAQIDHKGCRGHLLPLFCWGGVA MICPSLGLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.nAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGA GCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCC TCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCA CCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAG GGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTA ACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCT CCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCC ACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTC ATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGG CCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCG CCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCA CCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGT TTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCC CCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCT GCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAG CCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL QAGKGRH*EAGPGQAAQAASGESRDSAGRESEG AQRSANT*ETSGPTKGKQKQG*EGGRGGHLAGV LGGGAVTHACRLLLTGGAASFWDWTALLRSHRPH PFPRPTITTASGRHPHLPPVPSPPHYTAHQPLQ GSHGLSGEQFSPGLSSQLPPPTHAYTHALLDKA NIPLSRTLHLLRPHSLGGGDIALWAFGLGAPSLL LHCSLWLPIVGPGRVPLALKGAQAPSHPGTPYS TALAAAGVANGGGCWPPGFPQPNCLCHHVGLTF HPSPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCG HSPLALRLGSEYILW*WRCSHLLSR*GPRALWP PPEVGWGCSPNPTLLPPLSHFPLLRWGTNNKEL TLPAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVV FCSMFHSSTSVIAAATSAKCSSSLPPVLPTIPS PKILFVGKRGWGMAGWVTDYPSPREGGALPLGC CSRVSKGAQIDHKGCRGHLLPLFCWGGVAMICPS LGLPLWFPICSYLNKKNILFWTG

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA GGRARASQPSLFHLLLREGKMSESSSKSSQPLA SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKE PSEVPTPKRPRGRPKGSKNKGEKEEEEGISQES SEEEQ*PMRAACSSLEEQLPSGTGQLCSAPTAP TPSPGPPSPPPLAATPIFHLCPHHHTTQHTSRCR APMG*VGSSFPLASVPSSPRPPTHTHMPSWTRL TSHLAAPCTCCVPTPLVVGTLLSGLLVWGRPLC SFTVPSGFP*WGLGGFPWP*KGPKPHLILARPTP LPWQQQVWPMEGGAGPQDSPSQTVFVTTWGSLF ILPQLP*SPY*VGQPPSVTGRQEG*VPYSLFTV ATAPLPSAWDLSTYCGDGDAVTYCPGEAQEPCGR HLRWAGAAPLTLLCFRHSAISPSSDGAPITRSS PCPLPTPLLLLPAPQGSGSIFPLFTNYLWTVVL FFVQCSILRHPSLLLLPAPNVHPHCLLFCPRSPP PRYSLWGRGAGAWQAG*PTTPVPGKVGPCP*DA AAE*ARGPKSTIKGVGATSSPCSVGEG*P*FVP AWGSLSGFLFAVT*IKKISFSGLE

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRR SPAKCQHLRDLGADQREAKTRVRRRKRRASRRSP RRRSSDPCVPPAPHWRSSFLLGLDSFAPLPPPP PLPQAHHHHRLWPPPPSSTCALTTTLHSTPAAA GLPWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG* HPT*PHPAPAASPLPWWWGHCSLGFWFGGALSA PSLFPLASHSGAWEGSPGLKRGPSPISSWHALL HCPGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFS SFPNFPSPRTRLDSPLRLQEGRRGESPTPSSLW PQPPCPPPGI*VHIVVMEMQSLIVQVRPKSPVA AT*GGLGLLP*PYFASATQPFPPPQMGHQ*QGAH PARSQPPSCSSLPPKVLVPFFLCSQTTSGQLCC FLFNVPFFDIRHCCCYQRQMFILIASCSAHDPL PQDTLCGEEGLGHGRLGDRLPQSQGRWGPAPRML QQSEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQ PGAPSLVSYLQLLE*KKYPFLDW

Sequence HMGA1.mAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG GGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGA GGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGA CAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACC ACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCA TACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGG CTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGC CCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGT CTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGG TGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTC CAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTC AGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTC ACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATT GCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGG GAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTT GGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP GTALVGSQKEPSEVPTPKRPRGRPKGSKNKGEKE EEEGISQESSEEEQ*PMRAACSSLEEQLPSGTG QLCSAPTAPTPSPGPPSPPPLAATPIFHLCPHH HTTQHTSRCRAPMG*VGSSFPLASVPSSPRPPTH THMPSWTRLTSHLAAPCTCCVPTPLVVGTLLSG LLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGPK PHLILARPTPLPWQQQVWPMEGGAGPQDSPSQTV FVTTWGSLFILPQLP*SPY*VGQPPSVTGRQEG *VPYSLFTVATAPLPSAWDLSTYCGDGDAVTYC PGEAQEPCGRHLRWAGAAPLTLLCFRHSAISPSS DGAPITRSSPCPLPTPLLLLPAPQGSGSIFPLF TNYLWTVVLFFVQCSILRHPSLLLLPAPNVHPH CLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVPG KVGPCP*DAAAE*ARGPKSTIKGVGATSSPCSV GEG*P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP GQRW*GVRRSPAKCQHLRDLGADQREAKTRVRR RKRRASRRSPRRRSSDPCVPPAPHWRSSFLLGLD SFAPLPPPPPLPQAHHHHRLWPPPPSSTCALTT TLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHPR IHTCPPGQG*HPT*PHPAPAASPLPWWWGHCSLG FWFGGALSAPSLFPLASHSGAWEGSPGLKRGPS PISSWHALLHCPGSSRCGQWRGVLAPRIPPAKL SLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRRG ESPTPSSLWPQPPCPPPGI*VHIVVMEMQSLIV QVRPKSPVAAT*GGLGLLP*PYFASATQPFPPP QMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLCS QTTSGQLCCFLFNVPFFDIRHCCCYQRQMFILI ASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQSQ GRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVLL GRGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESEGAQRSANT*ETSGPTKGKQKQG*EG GRGGHLAGVLGGGAVTHACRLLLTGGAASFWDW TALLRSHRPHPFPRPTITTASGRHPHLPPVPSPP HYTAHQPLQGSHGLSGEQFSPGLSSQLPPPTHA YTHALLDKANIPLSRTLHLLRPHSLGGGDIALW AFGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQA PSHPGTPYSTALAAAGVANGGGCWPPGFPQPNC LCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAGG VSPLLPLHCGHSPLALRLGSEYILW*WRCSHLLS R*GPRALWPPPEVGWGCSPNPTLLPPLSHFPLL RWGTNNKELTLPAPNPPPAPPCPPRFWFHFSSV HKLPLDSCVVFCSMFHSSTSVIAAATSAKCSSSL PPVLPTIPSPKILFVGKRGWGMAGWVTDYPSPR EGGALPLGCCSRVSKGAQIDHKGCRGHLLPLFC WGGVAMICPSLGLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.vhAug05

CTGGAAAAATGTGTAGCCTAGGCCTCCGGCGTCTCATCCATCTGTAGCCTGGGGATAAGGACTAGGATGAAGATGTGGAGGACTGTCCTTTTTAAGCCTG TAATACAGGGTATTCCCAAATACAGGTTGCAGGCCCTCCTTCCAATGAGAAGTTTTAGGAAAACTCAAAATTGATGGGGTTAATTAAACCCCAACAGGCC TCTAAGAAGAAGGAAGATGCTAGAAAATGACCAAGAAGGTTTTAGGATGAGCCCTAAACCTCATCCTAAAGGATGAGGGCCAGCCAGGGTCTGTTAGGCG GTGCATTTCAGTTGGAGAATTCCCTACCCATCTGACAAAGTCCCATCTGACTCCAGTTGGGCAGAGGAGGGGTAAGGGGAAAGAGGAGGGTGGCCTTTGG CTTTGGGCTCACTTTCCTCCCCCACCTCACCCACTAGCTTCAACCATCTCCTCCCTCCCTCAATTATACCAATCTGAATTCTCCAGGGATTTACCCTGGG TGAGAACAGAGAAGAGAGCTGGTGGTGGGCACCACCCCTGGCTGTGTGATTGGCTCTTTTTGGTCCTGAGTCTTGCACCTTGCCAGATAGGCAATATTGT CAGTGAGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGG AAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGA AGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCA AGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCAC TGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCAC CCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCA GTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTC CCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGG GTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCT GGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGC CCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTG TGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGC TTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCC AAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCT GCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAG GCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTG TAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAAT AAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

LEKCVA*ASGVSSICSLGIRTRMKMWRTVLFKP VIQGIPKYRLQALLPMRSFRKTQN*WG*LNPNRP LRRRKMLENDQEGFRMSPKPHPKG*GPARVC*A VHFSWRIPYPSDKVPSDSSWAEEG*GERGGWPL ALGSLSSPTSPTSFNHLLPPSIIPI*ILQGFTLG ENREESWWWAPPLAV*LALFGPESCTLPDRQYC Q*ASQPSLFHLLLREGKMSESSSKSSQPLASKQ EKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSE VPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRKP RGRPKKLEKEEEEGISQESSEEEQ*PMRAACSS LEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLAAT PIFHLCPHHHTTQHTSRCRAPMG*VGSSFPLAS VPSSPRPPTHTHMPSWTRLTSHLAAPCTCCVPT PLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGLGG FPWP*KGPKPHLILARPTPLPWQQQVWPMEGGA GPQDSPSQTVFVTTWGSLFILPQLP*SPY*VGQ PPSVTGRQEG*VPYSLFTVATAPLPSAWDLSTYC GDGDAVTYCPGEAQEPCGRHLRWAGAAPLTLLC FRHSAISPSSDGAPITRSSPCPLPTPLLLLPAP QGSGSIFPLFTNYLWTVVLFFVQCSILRHPSLLL LPAPNVHPHCLLFCPRSPPPRYSLWGRGAGAWQ AG*PTTPVPGKVGPCP*DAAAE*ARGPKSTIKG VGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT*I KKISFSGLE

Frame 1

WKNV*PRPPASHPSVAWG*GLG*RCGGLSFLSL *YRVFPNTGCRPSFQ*EVLGKLKIDGVN*TPTG L*EEGRC*KMTKKVLG*ALNLILKDEGQPGSVRR CISVGEFPTHLTKSHLTPVGQRRGKGKEEGGLW LWAHFPPPPHPLASTISSLPQLYQSEFSRDLPW VRTEKRAGGGHHPWLCDWLFLVLSLAPCQIGNIV SEHPSHHSSTCSLEKGR*VSRARSPASPWPPSR KRTALRSGAGAGRASSLR*VPGQRW*GVRRSPA KCQHLRDLGADQREAKTRVLPRPGKPPQLQEGNQ GADPKNWRRRKRRASRRSPRRRSSDPCVPPAPH WRSSFLLGLDSFAPLPPPPPLPQAHHHHRLWPP PPSSTCALTTTLHSTPAAAGLPWAEWGAVFPWPQ FPAPPAHPRIHTCPPGQG*HPT*PHPAPAASPL PWWWGHCSLGFWFGGALSAPSLFPLASHSGAWE GSPGLKRGPSPISSWHALLHCPGSSRCGQWRGVL APRIPPAKLSLSPRGAHFSSFPNFPSPRTRLDS PLRLQEGRRGESPTPSSLWPQPPCPPPGI*VHI VVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PYFA SATQPFPPPQMGHQ*QGAHPARSQPPSCSSLPP KVLVPFFLCSQTTSGQLCCFLFNVPFFDIRHCC CYQRQMFILIASCSAHDPLPQDTLCGEEGLGHGR LGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV *GPPPPPVLLGRGSHDLSQPGAPSLVSYLQLLE *KKYPFLDW

Frame 2

GKMCSLGLRRLIHL*PGDKD*DEDVEDCPF*AC NTGYSQIQVAGPPSNEKF*ENSKLMGLIKPQQA SKKKEDARK*PRRF*DEP*TSS*RMRASQGLLG GAFQLENSLPI*QSPI*LQLGRGGVRGKRRVAFG FGLTFLPHLTH*LQPSPPSLNYTNLNSPGIYPG *EQRRELVVGTTPGCVIGSFWS*VLHLAR*AIL SVSIPAITLPPAP*RREDE*VELEVQPALGLQAG KGRH*EAGPGQAAQAASGESRDSAGRESEGAQR SANT*ETSGPTKGKQKQGCCQDPENHHNSRKET KGQTQKTGEGGRGGHLAGVLGGGAVTHACRLLLT GGAASFWDWTALLRSHRPHPFPRPTITTASGRH PHLPPVPSPPHYTAHQPLQGSHGLSGEQFSPGL SSQLPPPTHAYTHALLDKANIPLSRTLHLLRPHS LGGGDIALWAFGLGAPSLLLHCSLWLPIVGPGR VPLALKGAQAPSHPGTPYSTALAAAGVANGGGC WPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGWTA PFGYRKAGGVSPLLPLHCGHSPLALRLGSEYIL W*WRCSHLLSR*GPRALWPPPEVGWGCSPNPTL LPPLSHFPLLRWGTNNKELTLPAPNPPPAPPCPP RFWFHFSSVHKLPLDSCVVFCSMFHSSTSVIAA ATSAKCSSSLPPVLPTIPSPKILFVGKRGWGMA GWVTDYPSPREGGALPLGCCSRVSKGAQIDHKGC RGHLLPLFCWGGVAMICPSLGLPLWFPICSYLN KKNILFWTG

Sequence HMGA1.vfAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGCATCCCAGCCATCACTCT TCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGG CCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGG GGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGA AGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGAC TGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCAC CACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCA CGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTC TGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCC AAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAA CTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGA GGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTAT TGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCT CCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCT GTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCT CATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCC CAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTC TGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTG GAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSASQPSL FHLLLREGKMSESSSKSSQPLASKQEKDGTEKRG RGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPR GRPKGSKNKGAAKTRKTTTTPGRKPRGRPKKLE KEEEEGISQESSEEEQ*PMRAACSSLEEQLPSGT GQLCSAPTAPTPSPGPPSPPPLAATPIFHLCPH HHTTQHTSRCRAPMG*VGSSFPLASVPSSPRPP THTHMPSWTRLTSHLAAPCTCCVPTPLVVGTLLS GLLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGP KPHLILARPTPLPWQQQVWPMEGGAGPQDSPSQ TVFVTTWGSLFILPQLP*SPY*VGQPPSVTGRQE G*VPYSLFTVATAPLPSAWDLSTYCGDGDAVTY CPGEAQEPCGRHLRWAGAAPLTLLCFRHSAISP SSDGAPITRSSPCPLPTPLLLLPAPQGSGSIFPL FTNYLWTVVLFFVQCSILRHPSLLLLPAPNVHP HCLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPV PGKVGPCP*DAAAE*ARGPKSTIKGVGATSSPCS VGEG*P*FVPAWGSLSGFLFAVT*IKKISFSGL E

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPHPSHHS STCSLEKGR*VSRARSPASPWPPSRKRTALRSG AGAGRASSLR*VPGQRW*GVRRSPAKCQHLRDLG ADQREAKTRVLPRPGKPPQLQEGNQGADPKNWR RRKRRASRRSPRRRSSDPCVPPAPHWRSSFLLG LDSFAPLPPPPPLPQAHHHHRLWPPPPSSTCALT TTLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHP RIHTCPPGQG*HPT*PHPAPAASPLPWWWGHCS LGFWFGGALSAPSLFPLASHSGAWEGSPGLKRGP SPISSWHALLHCPGSSRCGQWRGVLAPRIPPAK LSLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGR RGESPTPSSLWPQPPCPPPGI*VHIVVMEMQSLI VQVRPKSPVAAT*GGLGLLP*PYFASATQPFPP PQMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFL CSQTTSGQLCCFLFNVPFFDIRHCCCYQRQMFIL IASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQS QGRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPV LLGRGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRIPAITL PPAP*RREDE*VELEVQPALGLQAGKGRH*EAG PGQAAQAASGESRDSAGRESEGAQRSANT*ETS GPTKGKQKQGCCQDPENHHNSRKETKGQTQKTGE GGRGGHLAGVLGGGAVTHACRLLLTGGAASFWD WTALLRSHRPHPFPRPTITTASGRHPHLPPVPS PPHYTAHQPLQGSHGLSGEQFSPGLSSQLPPPTH AYTHALLDKANIPLSRTLHLLRPHSLGGGDIAL WAFGLGAPSLLLHCSLWLPIVGPGRVPLALKGA QAPSHPGTPYSTALAAAGVANGGGCWPPGFPQPN CLCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAG GVSPLLPLHCGHSPLALRLGSEYILW*WRCSHL LSR*GPRALWPPPEVGWGCSPNPTLLPPLSHFPL LRWGTNNKELTLPAPNPPPAPPCPPRFWFHFSS VHKLPLDSCVVFCSMFHSSTSVIAAATSAKCSS SLPPVLPTIPSPKILFVGKRGWGMAGWVTDYPSP REGGALPLGCCSRVSKGAQIDHKGCRGHLLPLF CWGGVAMICPSLGLPLWFPICSYLNKKNILFWT G

Sequence HMGA1.vcAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCCATCCCAGCCATCACTCTTCCACCTGCTCCTT AGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGC GCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGG AAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAG GGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCT CCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACA GCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATG CCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTT GGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCAT CCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACC ACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCT ACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGC CCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCA CCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACC TCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTT CTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGG CCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGT AGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAAIPAITLPPAP *RREDE*VELEVQPALGLQAGKGRH*EAGPGQAA QAASGESRDSAGRESEGAQRSANT*ETSGPTKG KQKQGCCQDPENHHNSRKETKGQTQKTGEGGRG GHLAGVLGGGAVTHACRLLLTGGAASFWDWTALL RSHRPHPFPRPTITTASGRHPHLPPVPSPPHYT AHQPLQGSHGLSGEQFSPGLSSQLPPPTHAYTH ALLDKANIPLSRTLHLLRPHSLGGGDIALWAFGL GAPSLLLHCSLWLPIVGPGRVPLALKGAQAPSH PGTPYSTALAAAGVANGGGCWPPGFPQPNCLCH HVGLTFHPSPTSLVPVLGWTAPFGYRKAGGVSPL LPLHCGHSPLALRLGSEYILW*WRCSHLLSR*G PRALWPPPEVGWGCSPNPTLLPPLSHFPLLRWG TNNKELTLPAPNPPPAPPCPPRFWFHFSSVHKLP LDSCVVFCSMFHSSTSVIAAATSAKCSSSLPPV LPTIPSPKILFVGKRGWGMAGWVTDYPSPREGG ALPLGCCSRVSKGAQIDHKGCRGHLLPLFCWGGV AMICPSLGLPLWFPICSYLNKKNILFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLPSQPSLFHLLL REGKMSESSSKSSQPLASKQEKDGTEKRGRGRP RKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKG SKNKGAAKTRKTTTTPGRKPRGRPKKLEKEEEE GISQESSEEEQ*PMRAACSSLEEQLPSGTGQLC SAPTAPTPSPGPPSPPPLAATPIFHLCPHHHTTQ HTSRCRAPMG*VGSSFPLASVPSSPRPPTHTHM PSWTRLTSHLAAPCTCCVPTPLVVGTLLSGLLV WGRPLCSFTVPSGFP*WGLGGFPWP*KGPKPHLI LARPTPLPWQQQVWPMEGGAGPQDSPSQTVFVT TWGSLFILPQLP*SPY*VGQPPSVTGRQEG*VP YSLFTVATAPLPSAWDLSTYCGDGDAVTYCPGEA QEPCGRHLRWAGAAPLTLLCFRHSAISPSSDGA PITRSSPCPLPTPLLLLPAPQGSGSIFPLFTNY LWTVVLFFVQCSILRHPSLLLLPAPNVHPHCLLF CPRSPPPRYSLWGRGAGAWQAG*PTTPVPGKVG PCP*DAAAE*ARGPKSTIKGVGATSSPCSVGEG *P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCHPSHHSSTCSL EKGR*VSRARSPASPWPPSRKRTALRSGAGAGR ASSLR*VPGQRW*GVRRSPAKCQHLRDLGADQR EAKTRVLPRPGKPPQLQEGNQGADPKNWRRRKRR ASRRSPRRRSSDPCVPPAPHWRSSFLLGLDSFA PLPPPPPLPQAHHHHRLWPPPPSSTCALTTTLH STPAAAGLPWAEWGAVFPWPQFPAPPAHPRIHTC PPGQG*HPT*PHPAPAASPLPWWWGHCSLGFWF GGALSAPSLFPLASHSGAWEGSPGLKRGPSPIS SWHALLHCPGSSRCGQWRGVLAPRIPPAKLSLSP RGAHFSSFPNFPSPRTRLDSPLRLQEGRRGESP TPSSLWPQPPCPPPGI*VHIVVMEMQSLIVQVR PKSPVAAT*GGLGLLP*PYFASATQPFPPPQMGH Q*QGAHPARSQPPSCSSLPPKVLVPFFLCSQTT SGQLCCFLFNVPFFDIRHCCCYQRQMFILIASC SAHDPLPQDTLCGEEGLGHGRLGDRLPQSQGRWG PAPRMLQQSEQGGPNRP*RV*GPPPPPVLLGRG SHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Sequence HMGA1.tAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA GGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCA CCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACC CATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCA TCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGG GGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACC CTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGC TTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGG TGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTA GTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCC GCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGG CTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCC CCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATT CTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGG GAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGG GCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGT TTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV GSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKT TTTPGRKPRGRPKKLEKEEEEGISQESSEEEQ*P MRAACSSLEEQLPSGTGQLCSAPTAPTPSPGPP SPPPLAATPIFHLCPHHHTTQHTSRCRAPMG*V GSSFPLASVPSSPRPPTHTHMPSWTRLTSHLAAP CTCCVPTPLVVGTLLSGLLVWGRPLCSFTVPSG FP*WGLGGFPWP*KGPKPHLILARPTPLPWQQQ VWPMEGGAGPQDSPSQTVFVTTWGSLFILPQLP* SPY*VGQPPSVTGRQEG*VPYSLFTVATAPLPS AWDLSTYCGDGDAVTYCPGEAQEPCGRHLRWAG AAPLTLLCFRHSAISPSSDGAPITRSSPCPLPTP LLLLPAPQGSGSIFPLFTNYLWTVVLFFVQCSI LRHPSLLLLPAPNVHPHCLLFCPRSPPPRYSLW GRGAGAWQAG*PTTPVPGKVGPCP*DAAAE*ARG PKSTIKGVGATSSPCSVGEG*P*FVPAWGSLSG FLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW* GVRRSPAKCQHLRDLGADQREAKTRVLPRPGKP PQLQEGNQGADPKNWRRRKRRASRRSPRRRSSD PCVPPAPHWRSSFLLGLDSFAPLPPPPPLPQAHH HHRLWPPPPSSTCALTTTLHSTPAAAGLPWAEW GAVFPWPQFPAPPAHPRIHTCPPGQG*HPT*PH PAPAASPLPWWWGHCSLGFWFGGALSAPSLFPLA SHSGAWEGSPGLKRGPSPISSWHALLHCPGSSR CGQWRGVLAPRIPPAKLSLSPRGAHFSSFPNFP SPRTRLDSPLRLQEGRRGESPTPSSLWPQPPCPP PGI*VHIVVMEMQSLIVQVRPKSPVAAT*GGLG LLP*PYFASATQPFPPPQMGHQ*QGAHPARSQP PSCSSLPPKVLVPFFLCSQTTSGQLCCFLFNVPF FDIRHCCCYQRQMFILIASCSAHDPLPQDTLCG EEGLGHGRLGDRLPQSQGRWGPAPRMLQQSEQG GPNRP*RV*GPPPPPVLLGRGSHDLSQPGAPSLV SYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG RESEGAQRSANT*ETSGPTKGKQKQGCCQDPENH HNSRKETKGQTQKTGEGGRGGHLAGVLGGGAVT HACRLLLTGGAASFWDWTALLRSHRPHPFPRPT ITTASGRHPHLPPVPSPPHYTAHQPLQGSHGLSG EQFSPGLSSQLPPPTHAYTHALLDKANIPLSRT LHLLRPHSLGGGDIALWAFGLGAPSLLLHCSLW LPIVGPGRVPLALKGAQAPSHPGTPYSTALAAAG VANGGGCWPPGFPQPNCLCHHVGLTFHPSPTSL VPVLGWTAPFGYRKAGGVSPLLPLHCGHSPLAL RLGSEYILW*WRCSHLLSR*GPRALWPPPEVGWG CSPNPTLLPPLSHFPLLRWGTNNKELTLPAPNP PPAPPCPPRFWFHFSSVHKLPLDSCVVFCSMFH SSTSVIAAATSAKCSSSLPPVLPTIPSPKILFVG KRGWGMAGWVTDYPSPREGGALPLGCCSRVSKG AQIDHKGCRGHLLPLFCWGGVAMICPSLGLPLW FPICSYLNKKNILFWTG

Sequence HMGA1.kAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGATCCGCATTTGCTACCAG CGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATC CCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGC ACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACAC CTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACC CAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAG CTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCC ACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTC CCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTG GGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGC CTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGA TTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTT ACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAG ATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTC AGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGT TCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGC CAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACC GACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACC TCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATAT CCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSDPHLLP AAAAAEPGRSSAPSTAAPGNPERAPQAGGRARAS QPSLFHLLLREGKMSESSSKSSQPLASKQEKDG TEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPT PKRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRP KKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQ LPSGTGQLCSAPTAPTPSPGPPSPPPLAATPIF HLCPHHHTTQHTSRCRAPMG*VGSSFPLASVPSS PRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVV GTLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPW P*KGPKPHLILARPTPLPWQQQVWPMEGGAGPQD SPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSV TGRQEG*VPYSLFTVATAPLPSAWDLSTYCGDG DAVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRHS AISPSSDGAPITRSSPCPLPTPLLLLPAPQGSG SIFPLFTNYLWTVVLFFVQCSILRHPSLLLLPA PNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*P TTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGAT SSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKI SFSGLE

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPIRICYQ RRPRRSQAGPQRPAPPLPATRSAHRRPAAELAH PSHHSSTCSLEKGR*VSRARSPASPWPPSRKRTA LRSGAGAGRASSLR*VPGQRW*GVRRSPAKCQH LRDLGADQREAKTRVLPRPGKPPQLQEGNQGAD PKNWRRRKRRASRRSPRRRSSDPCVPPAPHWRSS FLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSS TCALTTTLHSTPAAAGLPWAEWGAVFPWPQFPA PPAHPRIHTCPPGQG*HPT*PHPAPAASPLPWWW GHCSLGFWFGGALSAPSLFPLASHSGAWEGSPG LKRGPSPISSWHALLHCPGSSRCGQWRGVLAPR IPPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLRL QEGRRGESPTPSSLWPQPPCPPPGI*VHIVVME MQSLIVQVRPKSPVAAT*GGLGLLP*PYFASAT QPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVLV PFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQR QMFILIASCSAHDPLPQDTLCGEEGLGHGRLGD RLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GPP PPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKY PFLDW

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRSAFATS GGRGGARPVLSAQHRRSRQPGARTAGRRPSSRI PAITLPPAP*RREDE*VELEVQPALGLQAGKGR H*EAGPGQAAQAASGESRDSAGRESEGAQRSANT *ETSGPTKGKQKQGCCQDPENHHNSRKETKGQT QKTGEGGRGGHLAGVLGGGAVTHACRLLLTGGA ASFWDWTALLRSHRPHPFPRPTITTASGRHPHLP PVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQL PPPTHAYTHALLDKANIPLSRTLHLLRPHSLGG GDIALWAFGLGAPSLLLHCSLWLPIVGPGRVPLA LKGAQAPSHPGTPYSTALAAAGVANGGGCWPPG FPQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFG YRKAGGVSPLLPLHCGHSPLALRLGSEYILW*WR CSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPL SHFPLLRWGTNNKELTLPAPNPPPAPPCPPRFW FHFSSVHKLPLDSCVVFCSMFHSSTSVIAAATSA KCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVT DYPSPREGGALPLGCCSRVSKGAQIDHKGCRGH LLPLFCWGGVAMICPSLGLPLWFPICSYLNKKNI LFWTG

Sequence HMGA1.fAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG GGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAA GACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAG GAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTT CCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCC CATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCC CACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCA CTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCC CTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTT CCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAG CCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCT GAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGC CCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTG TTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAG ATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCA GAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGG GCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP GTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAK TRKTTTTPGRKPRGRPKKLEKEEEEGISQESSE EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTP SPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAP MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSF TVPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVAT APLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFF VQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAW GSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP GQRW*GVRRSPAKCQHLRDLGADQREAKTRVLP RPGKPPQLQEGNQGADPKNWRRRKRRASRRSPRR RSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGL PWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS LFPLASHSGAWEGSPGLKRGPSPISSWHALLHC PGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT *GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQ DTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG APSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESEGAQRSANT*ETSGPTKGKQKQGCCQ DPENHHNSRKETKGQTQKTGEGGRGGHLAGVLG GGAVTHACRLLLTGGAASFWDWTALLRSHRPHPF PRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANI PLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHP SPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS PLALRLGSEYILW*WRCSHLLSR*GPRALWPPP EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTL PAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCS RVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG LPLWFPICSYLNKKNILFWTG

Sequence HMGA1.eAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCG GCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAG CTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGG ACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGA CCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGA GGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCC CCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCA TGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCA CTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACT GTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCT GGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCC CCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCC CCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGA GGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCC GCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTT CAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGAT ACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGA GTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGC TCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRSAFATSGGRGGARPVLSAQHRRSR QPGARTAGRRPSSRIPAITLPPAP*RREDE*VE LEVQPALGLQAGKGRH*EAGPGQAAQAASGESR DSAGRESEGAQRSANT*ETSGPTKGKQKQGCCQD PENHHNSRKETKGQTQKTGEGGRGGHLAGVLGG GAVTHACRLLLTGGAASFWDWTALLRSHRPHPF PRPTITTASGRHPHLPPVPSPPHYTAHQPLQGSH GLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTAL AAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS PLALRLGSEYILW*WRCSHLLSR*GPRALWPPPE VGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPKI LFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG LPLWFPICSYLNKKNILFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEDPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSESS SKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPG TALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAK TRKTTTTPGRKPRGRPKKLEKEEEEGISQESSEE EQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAP MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSH LAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFILP QLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPCP LPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE *ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG SLSGFLFAVT*IKKISFSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKIRICYQRRPRRSQAGPQRPAPPLP ATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLR*VPG QRW*GVRRSPAKCQHLRDLGADQREAKTRVLPR PGKPPQLQEGNQGADPKNWRRRKRRASRRSPRR RSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLP QAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPSL FPLASHSGAWEGSPGLKRGPSPISSWHALLHCP GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQP PCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT* GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLF NVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGA PSLVSYLQLLE*KKYPFLDW

Sequence HMGA1.cAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGA GCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGA AGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCT GCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCT GGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCC CTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCG TCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGG CCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAG GGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGG TTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAG TACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACC CTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCC CTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTC ATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGG GCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCA TAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGT TACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL QAGKGRH*EAGPGQAAQAASGESRDSAGRESEG AQRSANT*ETSGPTKGKQKQGCCQDPENHHNSR KETKGQTQKTGEGGRGGHLAGVLGGGAVTHACRL LLTGGAASFWDWTALLRSHRPHPFPRPTITTAS GRHPHLPPVPSPPHYTAHQPLQGSHGLSGEQFS PGLSSQLPPPTHAYTHALLDKANIPLSRTLHLLR PHSLGGGDIALWAFGLGAPSLLLHCSLWLPIVG PGRVPLALKGAQAPSHPGTPYSTALAAAGVANG GGCWPPGFPQPNCLCHHVGLTFHPSPTSLVPVLG WTAPFGYRKAGGVSPLLPLHCGHSPLALRLGSE YILW*WRCSHLLSR*GPRALWPPPEVGWGCSPN PTLLPPLSHFPLLRWGTNNKELTLPAPNPPPAPP CPPRFWFHFSSVHKLPLDSCVVFCSMFHSSTSV IAAATSAKCSSSLPPVLPTIPSPKILFVGKRGW GMAGWVTDYPSPREGGALPLGCCSRVSKGAQIDH KGCRGHLLPLFCWGGVAMICPSLGLPLWFPICS YLNKKNILFWTG

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA GGRARASQPSLFHLLLREGKMSESSSKSSQPLA SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKE PSEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPG RKPRGRPKKLEKEEEEGISQESSEEEQ*PMRAA CSSLEEQLPSGTGQLCSAPTAPTPSPGPPSPPPL AATPIFHLCPHHHTTQHTSRCRAPMG*VGSSFP LASVPSSPRPPTHTHMPSWTRLTSHLAAPCTCC VPTPLVVGTLLSGLLVWGRPLCSFTVPSGFP*WG LGGFPWP*KGPKPHLILARPTPLPWQQQVWPME GGAGPQDSPSQTVFVTTWGSLFILPQLP*SPY* VGQPPSVTGRQEG*VPYSLFTVATAPLPSAWDLS TYCGDGDAVTYCPGEAQEPCGRHLRWAGAAPLT LLCFRHSAISPSSDGAPITRSSPCPLPTPLLLL PAPQGSGSIFPLFTNYLWTVVLFFVQCSILRHPS LLLLPAPNVHPHCLLFCPRSPPPRYSLWGRGAG AWQAG*PTTPVPGKVGPCP*DAAAE*ARGPKST IKGVGATSSPCSVGEG*P*FVPAWGSLSGFLFAV T*IKKISFSGLE

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRR SPAKCQHLRDLGADQREAKTRVLPRPGKPPQLQE GNQGADPKNWRRRKRRASRRSPRRRSSDPCVPP APHWRSSFLLGLDSFAPLPPPPPLPQAHHHHRL WPPPPSSTCALTTTLHSTPAAAGLPWAEWGAVFP WPQFPAPPAHPRIHTCPPGQG*HPT*PHPAPAA SPLPWWWGHCSLGFWFGGALSAPSLFPLASHSG AWEGSPGLKRGPSPISSWHALLHCPGSSRCGQWR GVLAPRIPPAKLSLSPRGAHFSSFPNFPSPRTR LDSPLRLQEGRRGESPTPSSLWPQPPCPPPGI* VHIVVMEMQSLIVQVRPKSPVAAT*GGLGLLP*P YFASATQPFPPPQMGHQ*QGAHPARSQPPSCSS LPPKVLVPFFLCSQTTSGQLCCFLFNVPFFDIR HCCCYQRQMFILIASCSAHDPLPQDTLCGEEGLG HGRLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP *RV*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQ LLE*KKYPFLDW