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Sequences

Sequence HMGA1.vnAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT
ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG
TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC
AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC
GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT
CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGGAGAA
GGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACT
GGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACC
ACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCAC
GCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCT
GGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCA
AGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAAC
TGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAG
GGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATT
GTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTC
CTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTG
TTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTC
ATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCC
AGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCT
GTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGG
AG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL
YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC
PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF
RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG
RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL
QAGKGRH*EAGPGQAAQAASGESRDSAGRESGE
GGRGGHLAGVLGGGAVTHACRLLLTGGAASFWD
WTALLRSHRPHPFPRPTITTASGRHPHLPPVPSP
PHYTAHQPLQGSHGLSGEQFSPGLSSQLPPPTH
AYTHALLDKANIPLSRTLHLLRPHSLGGGDIAL
WAFGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQ
APSHPGTPYSTALAAAGVANGGGCWPPGFPQPN
CLCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAG
GVSPLLPLHCGHSPLALRLGSEYILW*WRCSHLL
SR*GPRALWPPPEVGWGCSPNPTLLPPLSHFPL
LRWGTNNKELTLPAPNPPPAPPCPPRFWFHFSS
VHKLPLDSCVVFCSMFHSSTSVIAAATSAKCSSS
LPPVLPTIPSPKILFVGKRGWGMAGWVTDYPSP
REGGALPLGCCSRVSKGAQIDHKGCRGHLLPLF
CWGGVAMICPSLGLPLWFPICSYLNKKNILFWTG

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY
IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF
VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS
DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA
GGRARASQPSLFHLLLREGKMSESSSKSSQPLA
SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQEK
EEEEGISQESSEEEQ*PMRAACSSLEEQLPSGT
GQLCSAPTAPTPSPGPPSPPPLAATPIFHLCPH
HHTTQHTSRCRAPMG*VGSSFPLASVPSSPRPPT
HTHMPSWTRLTSHLAAPCTCCVPTPLVVGTLLS
GLLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGP
KPHLILARPTPLPWQQQVWPMEGGAGPQDSPSQT
VFVTTWGSLFILPQLP*SPY*VGQPPSVTGRQE
G*VPYSLFTVATAPLPSAWDLSTYCGDGDAVTY
CPGEAQEPCGRHLRWAGAAPLTLLCFRHSAISPS
SDGAPITRSSPCPLPTPLLLLPAPQGSGSIFPL
FTNYLWTVVLFFVQCSILRHPSLLLLPAPNVHP
HCLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVP
GKVGPCP*DAAAE*ARGPKSTIKGVGATSSPCS
VGEG*P*FVPAWGSLSGFLFAVT*IKKISFSGL
E

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI
SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL
SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF
QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP
AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP
PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRR
RRKRRASRRSPRRRSSDPCVPPAPHWRSSFLLGL
DSFAPLPPPPPLPQAHHHHRLWPPPPSSTCALT
TTLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHP
RIHTCPPGQG*HPT*PHPAPAASPLPWWWGHCSL
GFWFGGALSAPSLFPLASHSGAWEGSPGLKRGP
SPISSWHALLHCPGSSRCGQWRGVLAPRIPPAK
LSLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRR
GESPTPSSLWPQPPCPPPGI*VHIVVMEMQSLI
VQVRPKSPVAAT*GGLGLLP*PYFASATQPFPP
PQMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLC
SQTTSGQLCCFLFNVPFFDIRHCCCYQRQMFIL
IASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQS
QGRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVL
LGRGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Sequence HMGA1.vlAug05

GGGTCCCCAGCAGCAAGAGGTGGGGGGAGGCACCAGATGGTATGAGAGCTTCCAGGGAGACCCGCCAAGATCTCCAGGCAGGCCGGGCCAGGCTCTCTGG
GATTCCGGACTGGCTTCGCCCAACTGGAACCCCCTCGATGAGGGGGCCCCAGGCAGACCTTATATGAGCATCCCAGCCATCACTCTTCCACCTGCTCCTT
AGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGC
GCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAA
AACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTG
GAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAG
TGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCC
CACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTG
AGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCC
GCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCT
CTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCA
GCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTT
CCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGC
CCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGC
TGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCA
ACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTT
CCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTT
GTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCA
AGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCT
CTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

GSPAARGGGRHQMV*ELPGRPAKISRQAGPGSL
GFRTGFAQLEPPR*GGPRQTLYEHPSHHSSTCSL
EKGR*VSRARSPASPWPPSRKRTALRSGAGAGR
ASSLRRSPAKCQHLRDLGADQREAKTRVLPRPG
KPPQLQEGNQGADPKNW
RRRKRRASRRSPRRRSS
DPCVPPAPHWRSSFLLGLDSFAPLPPPPPLPQA
HHHHRLWPPPPSSTCALTTTLHSTPAAAGLPWA
EWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT*P
HPAPAASPLPWWWGHCSLGFWFGGALSAPSLFP
LASHSGAWEGSPGLKRGPSPISSWHALLHCPGS
SRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFPNF
PSPRTRLDSPLRLQEGRRGESPTPSSLWPQPPC
PPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*GG
LGLLP*PYFASATQPFPPPQMGHQ*QGAHPARSQ
PPSCSSLPPKVLVPFFLCSQTTSGQLCCFLFNV
PFFDIRHCCCYQRQMFILIASCSAHDPLPQDTL
CGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQSEQ
GGPNRP*RV*GPPPPPVLLGRGSHDLSQPGAPS
LVSYLQLLE*KKYPFLDW

Frame 1

GPQQQEVGGGTRWYESFQGDPPRSPGRPGQALW
DSGLASPNWNPLDEGAPGRPYMSIPAITLPPAP
*RREDE*VELEVQPALGLQAGKGRH*EAGPGQAA
QAASEGAQRSANT*ETSGPTKGKQKQGCCQDPE
NHHNSRKETKGQTQKT
GEGGRGGHLAGVLGGGA
VTHACRLLLTGGAASFWDWTALLRSHRPHPFPRP
TITTASGRHPHLPPVPSPPHYTAHQPLQGSHGL
SGEQFSPGLSSQLPPPTHAYTHALLDKANIPLS
RTLHLLRPHSLGGGDIALWAFGLGAPSLLLHCSL
WLPIVGPGRVPLALKGAQAPSHPGTPYSTALAA
AGVANGGGCWPPGFPQPNCLCHHVGLTFHPSPT
SLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSPLA
LRLGSEYILW*WRCSHLLSR*GPRALWPPPEVG
WGCSPNPTLLPPLSHFPLLRWGTNNKELTLPAP
NPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCSMF
HSSTSVIAAATSAKCSSSLPPVLPTIPSPKILF
VGKRGWGMAGWVTDYPSPREGGALPLGCCSRVS
KGAQIDHKGCRGHLLPLFCWGGVAMICPSLGLPL
WFPICSYLNKKNILFWTG

Frame 2

VPSSKRWGEAPDGMRASRETRQDLQAGRARLSG
IPDWLRPTGTPSMRGPQADLI*ASQPSLFHLLL
REGKMSESSSKSSQPLASKQEKDGTEKRGRGRP
RKQPPKEPSEVPTPKRPRGRPKGSKNKGAAKTRK
TTTTPGRKPRGRPKKL
EKEEEEGISQESSEEEQ
*PMRAACSSLEEQLPSGTGQLCSAPTAPTPSPG
PPSPPPLAATPIFHLCPHHHTTQHTSRCRAPMG*
VGSSFPLASVPSSPRPPTHTHMPSWTRLTSHLA
APCTCCVPTPLVVGTLLSGLLVWGRPLCSFTVP
SGFP*WGLGGFPWP*KGPKPHLILARPTPLPWQQ
QVWPMEGGAGPQDSPSQTVFVTTWGSLFILPQL
P*SPY*VGQPPSVTGRQEG*VPYSLFTVATAPL
PSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLRWA
GAAPLTLLCFRHSAISPSSDGAPITRSSPCPLP
TPLLLLPAPQGSGSIFPLFTNYLWTVVLFFVQC
SILRHPSLLLLPAPNVHPHCLLFCPRSPPPRYSL
WGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE*A
RGPKSTIKGVGATSSPCSVGEG*P*FVPAWGSL
SGFLFAVT*IKKISFSGLE

Sequence HMGA1.vjAug05

GTTGGAACTAGTCCCTTTGACTCCCCGTTTCTATTTTATTTTCTTCTGGGGCTTTATTTTGCGGGGAGGGGACGGAGGGAGGGGGCTTTCTTCCTCTCCC
CCGCGCCCGCGCGCGCCAGGTTTCCTGCCCCAAGGCGGGCTTGGGTGCCCCCTCTGGCAGGAAGTGGGGCCGGGTGCACCCGCGTGGCGGTGCATGCGGT
CGGGGTGCGCTGCTTTCCCGCGCCTGCAGGAGCGGCCTGCGCCCCTCCCCCCGCGCGCCCTGGCCCCGGGTCCCGCCGGCCGGCCTGCAACGGCCGGGCG
CAGACACTTGCAGAGTCACCCTCGGGCCGGGTCTGGAGCCTGATGCCTCGGGGGTTTAGCCCTAGCCGCTAGGCGGCATCCCAGCCATCACTCTTCCACC
TGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGG
CAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAG
ACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTG
GAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGG
AGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTC
CCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCC
ATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCC
ACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCAC
TGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCC
TGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTC
CCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGC
CCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTG
AGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCC
CGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGT
TCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGA
TACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAG
AGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGG
CTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

VGTSPFDSPFLFYFLLGLYFAGRGRREGAFFLS
PAPARARFPAPRRAWVPPLAGSGAGCTRVAVHAV
GVRCFPAPAGAACAPPPARPGPGSRRPACNGRA
QTLAESPSGRVWSLMPRGFSPSR*AASQPSLFH
LLLREGKMSESSSKSSQPLASKQEKDGTEKRGRG
RPRKQPPKEPSEVPTPKRPRGRPKGSKNKGAAK
TRKTTTTPGRKPRGRPKKL
EKEEEEGISQESSE
EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS
PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAP
MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS
HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT
VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP
WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL
PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA
PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL
RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC
PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV
QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR
YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA
E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG
SLSGFLFAVT*IKKISFSGLE

Frame 1

LELVPLTPRFYFIFFWGFILRGGDGGRGLSSSP
PRPRAPGFLPQGGLGCPLWQEVGPGAPAWRCMR
SGCAAFPRLQERPAPLPPRALAPGPAGRPATAGR
RHLQSHPRAGSGA*CLGGLALAARRHPSHHSST
CSLEKGR*VSRARSPASPWPPSRKRTALRSGAG
AGRASSLRRSPAKCQHLRDLGADQREAKTRVLPR
PGKPPQLQEGNQGADPKNW
RRRKRRASRRSPRR
RSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL
PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP
WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP
T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS
LFPLASHSGAWEGSPGLKRGPSPISSWHALLHCP
GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF
PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ
PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*
GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA
RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL
FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD
TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ
SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG
APSLVSYLQLLE*KKYPFLDW

Frame 2

WN*SL*LPVSILFSSGALFCGEGTEGGGFLPLP
RARARQVSCPKAGLGAPSGRKWGRVHPRGGACG
RGALLSRACRSGLRPSPRAPWPRVPPAGLQRPG
ADTCRVTLGPGLEPDASGV*P*PLGGIPAITLPP
AP*RREDE*VELEVQPALGLQAGKGRH*EAGPG
QAAQAASEGAQRSANT*ETSGPTKGKQKQGCCQ
DPENHHNSRKETKGQTQKT
GEGGRGGHLAGVLGG
GAVTHACRLLLTGGAASFWDWTALLRSHRPHPF
PRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS
HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP
LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH
CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA
LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS
PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS
PLALRLGSEYILW*WRCSHLLSR*GPRALWPPP
EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP
APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC
SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK
ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR
VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG
LPLWFPICSYLNKKNILFWTG

Sequence HMGA1.vgAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCC
AGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACC
TAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCC
AAAAAACTG
GAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGC
TTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCA
CCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCC
CCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGG
GGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCC
TTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGAT
TCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTA
CAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGA
TGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCA
GCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTT
CCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCC
AAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCG
ACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCT
CCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATC
CTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRSIPAITLPPAP*RREDE*VELEVQP
ALGLQAGKGRH*EAGPGQAAQAASEGAQRSANT
*ETSGPTKGKQKQGCCQDPENHHNSRKETKGQT
QKT
GEGGRGGHLAGVLGGGAVTHACRLLLTGGAA
SFWDWTALLRSHRPHPFPRPTITTASGRHPHLP
PVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQL
PPPTHAYTHALLDKANIPLSRTLHLLRPHSLGGG
DIALWAFGLGAPSLLLHCSLWLPIVGPGRVPLA
LKGAQAPSHPGTPYSTALAAAGVANGGGCWPPG
FPQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFGY
RKAGGVSPLLPLHCGHSPLALRLGSEYILW*WR
CSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPL
SHFPLLRWGTNNKELTLPAPNPPPAPPCPPRFWF
HFSSVHKLPLDSCVVFCSMFHSSTSVIAAATSA
KCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVT
DYPSPREGGALPLGCCSRVSKGAQIDHKGCRGHL
LPLFCWGGVAMICPSLGLPLWFPICSYLNKKNI
LFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEASQPSLFHLLLREGKMSESSSKSS
QPLASKQEKDGTEKRGRGRPRKQPPKEPSEVPTP
KRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRP
KKL
EKEEEEGISQESSEEEQ*PMRAACSSLEEQ
LPSGTGQLCSAPTAPTPSPGPPSPPPLAATPIFH
LCPHHHTTQHTSRCRAPMG*VGSSFPLASVPSS
PRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVV
GTLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPWP
*KGPKPHLILARPTPLPWQQQVWPMEGGAGPQD
SPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSV
TGRQEG*VPYSLFTVATAPLPSAWDLSTYCGDGD
AVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRHS
AISPSSDGAPITRSSPCPLPTPLLLLPAPQGSG
SIFPLFTNYLWTVVLFFVQCSILRHPSLLLLPAP
NVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*P
TTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGAT
SSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKIS
FSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKHPSHHSSTCSLEKGR*VSRARSPA
SPWPPSRKRTALRSGAGAGRASSLRRSPAKCQH
LRDLGADQREAKTRVLPRPGKPPQLQEGNQGADP
KNW
RRRKRRASRRSPRRRSSDPCVPPAPHWRSS
FLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSS
TCALTTTLHSTPAAAGLPWAEWGAVFPWPQFPAP
PAHPRIHTCPPGQG*HPT*PHPAPAASPLPWWW
GHCSLGFWFGGALSAPSLFPLASHSGAWEGSPG
LKRGPSPISSWHALLHCPGSSRCGQWRGVLAPRI
PPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLRL
QEGRRGESPTPSSLWPQPPCPPPGI*VHIVVME
MQSLIVQVRPKSPVAAT*GGLGLLP*PYFASATQ
PFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVLV
PFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQR
QMFILIASCSAHDPLPQDTLCGEEGLGHGRLGDR
LPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GPP
PPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKY
PFLDW

Sequence HMGA1.vdAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG
CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACA
CCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGAC
CCAAAAAACTG
GAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCA
GCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTC
CACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCT
CCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGT
GGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGG
CCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGG
ATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGT
TACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGA
GATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACT
CAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGG
TTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCG
CCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGAC
CGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCAC
CTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATA
TCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS
QPLASKQEKDGTEKRGRGRPRKQPPKEPSEVPT
PKRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGR
PKKL
EKEEEEGISQESSEEEQ*PMRAACSSLEEQ
LPSGTGQLCSAPTAPTPSPGPPSPPPLAATPIF
HLCPHHHTTQHTSRCRAPMG*VGSSFPLASVPS
SPRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVV
GTLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPW
P*KGPKPHLILARPTPLPWQQQVWPMEGGAGPQ
DSPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSV
TGRQEG*VPYSLFTVATAPLPSAWDLSTYCGDG
DAVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRH
SAISPSSDGAPITRSSPCPLPTPLLLLPAPQGSG
SIFPLFTNYLWTVVLFFVQCSILRHPSLLLLPA
PNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*
PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGAT
SSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKI
SFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP
ASPWPPSRKRTALRSGAGAGRASSLRRSPAKCQH
LRDLGADQREAKTRVLPRPGKPPQLQEGNQGAD
PKNW
RRRKRRASRRSPRRRSSDPCVPPAPHWRS
SFLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSS
TCALTTTLHSTPAAAGLPWAEWGAVFPWPQFPA
PPAHPRIHTCPPGQG*HPT*PHPAPAASPLPWW
WGHCSLGFWFGGALSAPSLFPLASHSGAWEGSPG
LKRGPSPISSWHALLHCPGSSRCGQWRGVLAPR
IPPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLR
LQEGRRGESPTPSSLWPQPPCPPPGI*VHIVVME
MQSLIVQVRPKSPVAAT*GGLGLLP*PYFASAT
QPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVL
VPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQR
QMFILIASCSAHDPLPQDTLCGEEGLGHGRLGD
RLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GP
PPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKY
PFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ
PALGLQAGKGRH*EAGPGQAAQAASEGAQRSAN
T*ETSGPTKGKQKQGCCQDPENHHNSRKETKGQT
QKT
GEGGRGGHLAGVLGGGAVTHACRLLLTGGA
ASFWDWTALLRSHRPHPFPRPTITTASGRHPHL
PPVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQL
PPPTHAYTHALLDKANIPLSRTLHLLRPHSLGG
GDIALWAFGLGAPSLLLHCSLWLPIVGPGRVPL
ALKGAQAPSHPGTPYSTALAAAGVANGGGCWPPG
FPQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFG
YRKAGGVSPLLPLHCGHSPLALRLGSEYILW*W
RCSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPL
SHFPLLRWGTNNKELTLPAPNPPPAPPCPPRFW
FHFSSVHKLPLDSCVVFCSMFHSSTSVIAAATS
AKCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVT
DYPSPREGGALPLGCCSRVSKGAQIDHKGCRGH
LLPLFCWGGVAMICPSLGLPLWFPICSYLNKKN
ILFWTG

Sequence HMGA1.vbAug05

CAGGCTGGTCTCGAACTCCTGACCTCAAGCAGTCCTCCTGCTTTGGCATCCCAAAGTGCTGGGATTACAGCATCCCAGCCATCACTCTTCCACCTGCTCC
TTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCC
GCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGG
AAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTG
GAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGC
AGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGG
CCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGC
TGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAG
CCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCC
CTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAG
CAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAAC
TTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTT
GCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGG
GCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCC
CAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATG
TTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCT
TTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAG
CAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCT
CTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

QAGLELLTSSSPPALASQSAGITASQPSLFHLL
LREGKMSESSSKSSQPLASKQEKDGTEKRGRGRP
RKQPPKEPSEVPTPKRPRGRPKGSKNKGAAKTR
KTTTTPGRKPRGRPKKL
EKEEEEGISQESSEEE
Q*PMRAACSSLEEQLPSGTGQLCSAPTAPTPSPG
PPSPPPLAATPIFHLCPHHHTTQHTSRCRAPMG
*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSHL
AAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFTVP
SGFP*WGLGGFPWP*KGPKPHLILARPTPLPWQ
QQVWPMEGGAGPQDSPSQTVFVTTWGSLFILPQ
LP*SPY*VGQPPSVTGRQEG*VPYSLFTVATAPL
PSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLRW
AGAAPLTLLCFRHSAISPSSDGAPITRSSPCPL
PTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFVQC
SILRHPSLLLLPAPNVHPHCLLFCPRSPPPRYS
LWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE*
ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWGSL
SGFLFAVT*IKKISFSGLE

Frame 1

RLVSNS*PQAVLLLWHPKVLGLQHPSHHSSTCS
LEKGR*VSRARSPASPWPPSRKRTALRSGAGAG
RASSLRRSPAKCQHLRDLGADQREAKTRVLPRPG
KPPQLQEGNQGADPKNW
RRRKRRASRRSPRRRS
SDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLPQ
AHHHHRLWPPPPSSTCALTTTLHSTPAAAGLPWA
EWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT*
PHPAPAASPLPWWWGHCSLGFWFGGALSAPSLF
PLASHSGAWEGSPGLKRGPSPISSWHALLHCPGS
SRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFPN
FPSPRTRLDSPLRLQEGRRGESPTPSSLWPQPP
CPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*GG
LGLLP*PYFASATQPFPPPQMGHQ*QGAHPARS
QPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLFN
VPFFDIRHCCCYQRQMFILIASCSAHDPLPQDTL
CGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQSE
QGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGAP
SLVSYLQLLE*KKYPFLDW

Frame 2

GWSRTPDLKQSSCFGIPKCWDYSIPAITLPPAP
*RREDE*VELEVQPALGLQAGKGRH*EAGPGQA
AQAASEGAQRSANT*ETSGPTKGKQKQGCCQDP
ENHHNSRKETKGQTQKT
GEGGRGGHLAGVLGGGA
VTHACRLLLTGGAASFWDWTALLRSHRPHPFPR
PTITTASGRHPHLPPVPSPPHYTAHQPLQGSHG
LSGEQFSPGLSSQLPPPTHAYTHALLDKANIPLS
RTLHLLRPHSLGGGDIALWAFGLGAPSLLLHCS
LWLPIVGPGRVPLALKGAQAPSHPGTPYSTALA
AAGVANGGGCWPPGFPQPNCLCHHVGLTFHPSPT
SLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSPL
ALRLGSEYILW*WRCSHLLSR*GPRALWPPPEV
GWGCSPNPTLLPPLSHFPLLRWGTNNKELTLPAP
NPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCSM
FHSSTSVIAAATSAKCSSSLPPVLPTIPSPKIL
FVGKRGWGMAGWVTDYPSPREGGALPLGCCSRVS
KGAQIDHKGCRGHLLPLFCWGGVAMICPSLGLP
LWFPICSYLNKKNILFWTG

Sequence HMGA1.pAug05

GAGCGCGAGTTGTGCACCCGGCGGAGCCCGGAGGAGCCCGGCGCACCTCGCGCGGCGGCCGCTCCCGCTGGAGCCGGAGCCCGAGCCCGAGCCCGGGCCC
GGGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAA
GGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAG
GGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTG
GAGAAGGAGGAAGAGG
AGGGCATCTC
GCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTG
CTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACA
CAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACA
TGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGT
TTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTC
ATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCA
CCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCC
CTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAG
GCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGG
CACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTA
CCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTG
TTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGG
GGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGG
GTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

ERELCTRRSPEEPGAPRAAAAPAGAGARARARA
RASQPSLFHLLLREGKMSESSSKSSQPLASKQEK
DGTEKRGRGRPRKQPPKEPSEVPTPKRPRGRPK
GSKNKGAAKTRKTTTTPGRKPRGRPKKL
EKEEE
EGIS
QESSEEEQ*PMRAACSSLEEQLPSGTGQLC
SAPTAPTPSPGPPSPPPLAATPIFHLCPHHHTT
QHTSRCRAPMG*VGSSFPLASVPSSPRPPTHTH
MPSWTRLTSHLAAPCTCCVPTPLVVGTLLSGLLV
WGRPLCSFTVPSGFP*WGLGGFPWP*KGPKPHL
ILARPTPLPWQQQVWPMEGGAGPQDSPSQTVFV
TTWGSLFILPQLP*SPY*VGQPPSVTGRQEG*VP
YSLFTVATAPLPSAWDLSTYCGDGDAVTYCPGE
AQEPCGRHLRWAGAAPLTLLCFRHSAISPSSDG
APITRSSPCPLPTPLLLLPAPQGSGSIFPLFTNY
LWTVVLFFVQCSILRHPSLLLLPAPNVHPHCLL
FCPRSPPPRYSLWGRGAGAWQAG*PTTPVPGKV
GPCP*DAAAE*ARGPKSTIKGVGATSSPCSVGEG
*P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 1

SASCAPGGARRSPAHLARRPLPLEPEPEPEPGP
GHPSHHSSTCSLEKGR*VSRARSPASPWPPSRK
RTALRSGAGAGRASSLRRSPAKCQHLRDLGADQR
EAKTRVLPRPGKPPQLQEGNQGADPKNW
RRRKR
RAS
RRSPRRRSSDPCVPPAPHWRSSFLLGLDSF
APLPPPPPLPQAHHHHRLWPPPPSSTCALTTTLH
STPAAAGLPWAEWGAVFPWPQFPAPPAHPRIHT
CPPGQG*HPT*PHPAPAASPLPWWWGHCSLGFW
FGGALSAPSLFPLASHSGAWEGSPGLKRGPSPIS
SWHALLHCPGSSRCGQWRGVLAPRIPPAKLSLS
PRGAHFSSFPNFPSPRTRLDSPLRLQEGRRGES
PTPSSLWPQPPCPPPGI*VHIVVMEMQSLIVQVR
PKSPVAAT*GGLGLLP*PYFASATQPFPPPQMG
HQ*QGAHPARSQPPSCSSLPPKVLVPFFLCSQT
TSGQLCCFLFNVPFFDIRHCCCYQRQMFILIASC
SAHDPLPQDTLCGEEGLGHGRLGDRLPQSQGRW
GPAPRMLQQSEQGGPNRP*RV*GPPPPPVLLGR
GSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

ARVVHPAEPGGARRTSRGGRSRWSRSPSPSPGP
GIPAITLPPAP*RREDE*VELEVQPALGLQAGK
GRH*EAGPGQAAQAASEGAQRSANT*ETSGPTK
GKQKQGCCQDPENHHNSRKETKGQTQKT
GEGGRG
GHL
AGVLGGGAVTHACRLLLTGGAASFWDWTAL
LRSHRPHPFPRPTITTASGRHPHLPPVPSPPHY
TAHQPLQGSHGLSGEQFSPGLSSQLPPPTHAYTH
ALLDKANIPLSRTLHLLRPHSLGGGDIALWAFG
LGAPSLLLHCSLWLPIVGPGRVPLALKGAQAPS
HPGTPYSTALAAAGVANGGGCWPPGFPQPNCLCH
HVGLTFHPSPTSLVPVLGWTAPFGYRKAGGVSP
LLPLHCGHSPLALRLGSEYILW*WRCSHLLSR*
GPRALWPPPEVGWGCSPNPTLLPPLSHFPLLRWG
TNNKELTLPAPNPPPAPPCPPRFWFHFSSVHKL
PLDSCVVFCSMFHSSTSVIAAATSAKCSSSLPP
VLPTIPSPKILFVGKRGWGMAGWVTDYPSPREGG
ALPLGCCSRVSKGAQIDHKGCRGHLLPLFCWGG
VAMICPSLGLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.jAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA
GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAA
ACCAAGGGGCAGACCCAAAAAACTG
GAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCC
TCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATG
GAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCA
CTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCT
GGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAG
CGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTG
ACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCC
ACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAA
TATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP
SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRK
PRGRPKKL
EKEEEEGISQESSEEEQ*PMRAACS
SLEEQLPSGTGQLCSAPTAPLPSAWDLSTYCGD
GDAVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRH
SAISPSSDGAPITRSSPCPLPTPLLLLPAPQGS
GSIFPLFTNYLWTVVLFFVQCSILRHPSLLLLP
APNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*
PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGA
TSSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKK
ISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP
AKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG
NQGADPKNW
RRRKRRASRRSPRRRSSDPCVPPAP
HWRSSFLLGLDSFAPLPQPPCPPPGI*VHIVVM
EMQSLIVQVRPKSPVAAT*GGLGLLP*PYFASA
TQPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVL
VPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQ
RQMFILIASCSAHDPLPQDTLCGEEGLGHGRLG
DRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GP
PPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KK
YPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ
RSANT*ETSGPTKGKQKQGCCQDPENHHNSRKE
TKGQTQKT
GEGGRGGHLAGVLGGGAVTHACRLL
LTGGAASFWDWTALLRSHSPLALRLGSEYILW*W
RCSHLLSR*GPRALWPPPEVGWGCSPNPTLLPP
LSHFPLLRWGTNNKELTLPAPNPPPAPPCPPRF
WFHFSSVHKLPLDSCVVFCSMFHSSTSVIAAATS
AKCSSSLPPVLPTIPSPKILFVGKRGWGMAGWV
TDYPSPREGGALPLGCCSRVSKGAQIDHKGCRG
HLLPLFCWGGVAMICPSLGLPLWFPICSYLNKKN
ILFWTG

Sequence HMGA1.iAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA
GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAA
ACCAAGGGGCAGACCCAAAAAACTG
GAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCC
TCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCG
CCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGC
CTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCC
ACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGG
GAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGG
TGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGA
CAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACAT
ATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACT
TTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCC
CCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGC
TGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATG
GCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAG
GGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTT
GAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP
SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRK
PRGRPKKL
EKEEEEGISQESSEEEQ*PMRAACS
SLEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLA
ATPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPLA
SVPSSPRPPTHTHMPSWTRLTSHLAAPCTCCVP
TPLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGL
GGFPWP*KGPKPHLILARPTPLPWQQQVWPMEGG
AGPQDSPSQTVFVTTWGSLFILPQLP*SPY*VG
QPPSVTGRQEG*VPYSLFTVATAPLPSAWDLST
YCGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTLL
CFRHSAISPSSDGAPITRSSPCPLPTPLLLLPA
PQGSGSIFPLFTNYLWTVVLFFVQCSILRHPSL
LLLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGAW
QAG*PTTPVPGKVGPCP*DAAAE*ARGPKSTIK
GVGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT
*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP
AKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG
NQGADPKNW
RRRKRRASRRSPRRRSSDPCVPPAP
HWRSSFLLGLDSFAPLPPPPPLPQAHHHHRLWP
PPPSSTCALTTTLHSTPAAAGLPWAEWGAVFPW
PQFPAPPAHPRIHTCPPGQG*HPT*PHPAPAASP
LPWWWGHCSLGFWFGGALSAPSLFPLASHSGAW
EGSPGLKRGPSPISSWHALLHCPGSSRCGQWRG
VLAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRLD
SPLRLQEGRRGESPTPSSLWPQPPCPPPGI*VH
IVVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PY
FASATQPFPPPQMGHQ*QGAHPARSQPPSCSSLP
PKVLVPFFLCSQTTSGQLCCFLFNVPFFDIRHC
CCYQRQMFILIASCSAHDPLPQDTLCGEEGLGH
GRLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*R
V*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQLL
E*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ
RSANT*ETSGPTKGKQKQGCCQDPENHHNSRKE
TKGQTQKT
GEGGRGGHLAGVLGGGAVTHACRLL
LTGGAASFWDWTALLRSHRPHPFPRPTITTASGR
HPHLPPVPSPPHYTAHQPLQGSHGLSGEQFSPG
LSSQLPPPTHAYTHALLDKANIPLSRTLHLLRP
HSLGGGDIALWAFGLGAPSLLLHCSLWLPIVGPG
RVPLALKGAQAPSHPGTPYSTALAAAGVANGGG
CWPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGW
TAPFGYRKAGGVSPLLPLHCGHSPLALRLGSEYI
LW*WRCSHLLSR*GPRALWPPPEVGWGCSPNPT
LLPPLSHFPLLRWGTNNKELTLPAPNPPPAPPC
PPRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVIA
AATSAKCSSSLPPVLPTIPSPKILFVGKRGWGM
AGWVTDYPSPREGGALPLGCCSRVSKGAQIDHK
GCRGHLLPLFCWGGVAMICPSLGLPLWFPICSYL
NKKNILFWTG

Sequence HMGA1.hAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT
ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG
TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC
AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC
GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT
CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCG
GGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTG
GAG
AAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGA
CTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCA
CCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCC
ACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCT
CTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCC
CAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAA
ACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGG
AGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTA
TTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCC
TCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTC
TGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCC
TCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTC
CCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTT
CTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACT
GGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Exon skipping and alternative donor or acceptor

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL
YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC
PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF
RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG
RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL
QAGKGRH*EAGPGQAAQAASEGAQRSANT*ETS
GPTKGKQKQGCCQDPENHHNSRKETKGQTQKT
G
EGGRGGHLAGVLGGGAVTHACRLLLTGGAASFWD
WTALLRSHRPHPFPRPTITTASGRHPHLPPVPS
PPHYTAHQPLQGSHGLSGEQFSPGLSSQLPPPT
HAYTHALLDKANIPLSRTLHLLRPHSLGGGDIAL
WAFGLGAPSLLLHCSLWLPIVGPGRVPLALKGA
QAPSHPGTPYSTALAAAGVANGGGCWPPGFPQP
NCLCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAG
GVSPLLPLHCGHSPLALRLGSEYILW*WRCSHL
LSR*GPRALWPPPEVGWGCSPNPTLLPPLSHFP
LLRWGTNNKELTLPAPNPPPAPPCPPRFWFHFSS
VHKLPLDSCVVFCSMFHSSTSVIAAATSAKCSS
SLPPVLPTIPSPKILFVGKRGWGMAGWVTDYPS
PREGGALPLGCCSRVSKGAQIDHKGCRGHLLPLF
CWGGVAMICPSLGLPLWFPICSYLNKKNILFWT
G

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY
IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF
VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS
DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA
GGRARASQPSLFHLLLREGKMSESSSKSSQPLA
SKQEKDGTEKRGRGRPRKQPPKEPSEVPTPKRPR
GRPKGSKNKGAAKTRKTTTTPGRKPRGRPKKL
E
KEEEEGISQESSEEEQ*PMRAACSSLEEQLPSG
TGQLCSAPTAPTPSPGPPSPPPLAATPIFHLCPH
HHTTQHTSRCRAPMG*VGSSFPLASVPSSPRPP
THTHMPSWTRLTSHLAAPCTCCVPTPLVVGTLL
SGLLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGP
KPHLILARPTPLPWQQQVWPMEGGAGPQDSPSQ
TVFVTTWGSLFILPQLP*SPY*VGQPPSVTGRQ
EG*VPYSLFTVATAPLPSAWDLSTYCGDGDAVTY
CPGEAQEPCGRHLRWAGAAPLTLLCFRHSAISP
SSDGAPITRSSPCPLPTPLLLLPAPQGSGSIFP
LFTNYLWTVVLFFVQCSILRHPSLLLLPAPNVHP
HCLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPV
PGKVGPCP*DAAAE*ARGPKSTIKGVGATSSPC
SVGEG*P*FVPAWGSLSGFLFAVT*IKKISFSGL
E

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI
SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL
SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF
QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP
AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP
PSRKRTALRSGAGAGRASSLRRSPAKCQHLRDL
GADQREAKTRVLPRPGKPPQLQEGNQGADPKNW
R
RRKRRASRRSPRRRSSDPCVPPAPHWRSSFLLG
LDSFAPLPPPPPLPQAHHHHRLWPPPPSSTCAL
TTTLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHP
RIHTCPPGQG*HPT*PHPAPAASPLPWWWGHCS
LGFWFGGALSAPSLFPLASHSGAWEGSPGLKRG
PSPISSWHALLHCPGSSRCGQWRGVLAPRIPPAK
LSLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGR
RGESPTPSSLWPQPPCPPPGI*VHIVVMEMQSL
IVQVRPKSPVAAT*GGLGLLP*PYFASATQPFPP
PQMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFL
CSQTTSGQLCCFLFNVPFFDIRHCCCYQRQMFI
LIASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQS
QGRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPV
LLGRGSHDLSQPGAPSLVSYLQLLE*KKYPFLD
W

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