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Sequences

Sequence HMGA1.vnAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGGAGAA GGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACT GGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACC ACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCAC GCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCT GGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCA AGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAAC TGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAG GGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATT GTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTC CTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTG TTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTC ATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCC AGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCT GTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGG AG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL QAGKGRH*EAGPGQAAQAASGESRDSAGRESGE GGRGGHLAGVLGGGAVTHACRLLLTGGAASFWD WTALLRSHRPHPFPRPTITTASGRHPHLPPVPSP PHYTAHQPLQGSHGLSGEQFSPGLSSQLPPPTH AYTHALLDKANIPLSRTLHLLRPHSLGGGDIAL WAFGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQ APSHPGTPYSTALAAAGVANGGGCWPPGFPQPN CLCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAG GVSPLLPLHCGHSPLALRLGSEYILW*WRCSHLL SR*GPRALWPPPEVGWGCSPNPTLLPPLSHFPL LRWGTNNKELTLPAPNPPPAPPCPPRFWFHFSS VHKLPLDSCVVFCSMFHSSTSVIAAATSAKCSSS LPPVLPTIPSPKILFVGKRGWGMAGWVTDYPSP REGGALPLGCCSRVSKGAQIDHKGCRGHLLPLF CWGGVAMICPSLGLPLWFPICSYLNKKNILFWTG

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA GGRARASQPSLFHLLLREGKMSESSSKSSQPLA SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQEK EEEEGISQESSEEEQ*PMRAACSSLEEQLPSGT GQLCSAPTAPTPSPGPPSPPPLAATPIFHLCPH HHTTQHTSRCRAPMG*VGSSFPLASVPSSPRPPT HTHMPSWTRLTSHLAAPCTCCVPTPLVVGTLLS GLLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGP KPHLILARPTPLPWQQQVWPMEGGAGPQDSPSQT VFVTTWGSLFILPQLP*SPY*VGQPPSVTGRQE G*VPYSLFTVATAPLPSAWDLSTYCGDGDAVTY CPGEAQEPCGRHLRWAGAAPLTLLCFRHSAISPS SDGAPITRSSPCPLPTPLLLLPAPQGSGSIFPL FTNYLWTVVLFFVQCSILRHPSLLLLPAPNVHP HCLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVP GKVGPCP*DAAAE*ARGPKSTIKGVGATSSPCS VGEG*P*FVPAWGSLSGFLFAVT*IKKISFSGL E

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRR RRKRRASRRSPRRRSSDPCVPPAPHWRSSFLLGL DSFAPLPPPPPLPQAHHHHRLWPPPPSSTCALT TTLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHP RIHTCPPGQG*HPT*PHPAPAASPLPWWWGHCSL GFWFGGALSAPSLFPLASHSGAWEGSPGLKRGP SPISSWHALLHCPGSSRCGQWRGVLAPRIPPAK LSLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRR GESPTPSSLWPQPPCPPPGI*VHIVVMEMQSLI VQVRPKSPVAAT*GGLGLLP*PYFASATQPFPP PQMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLC SQTTSGQLCCFLFNVPFFDIRHCCCYQRQMFIL IASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQS QGRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVL LGRGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Sequence HMGA1.qAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGA GGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCT TCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTC CCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATC CCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTC ACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGC CCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCT TCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACA GCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACC TGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTG CCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTT GTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAA GATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGC AGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGG GGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP SEVPTPKRPRGRPKGSKNKGEKEEEEGISQESSE EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTP SPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRA PMG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSF TVPSGFP*WGLGGFPWP*KGPKPHLILARPTPL PWQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVAT APLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRH LRWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFF VQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPP RYSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAW GSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP AKCQHLRDLGADQREAKTRVRRRKRRASRRSPR RRSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGL PWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*H PT*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS LFPLASHSGAWEGSPGLKRGPSPISSWHALLHC PGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSS FPNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT *GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHP ARSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQ DTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQ QSEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG APSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ RSANT*ETSGPTKGKQKQG*EGGRGGHLAGVLG GGAVTHACRLLLTGGAASFWDWTALLRSHRPHP FPRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANI PLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLL HCSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHP SPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGH SPLALRLGSEYILW*WRCSHLLSR*GPRALWPPP EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTL PAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVF CSMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCS RVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSL GLPLWFPICSYLNKKNILFWTG