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Sequences

Sequence HMGA1.vnAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT
ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG
TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC
AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC
GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT
CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGGAGAA
GGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACT
GGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACC
ACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCAC
GCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCT
GGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCA
AGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAAC
TGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAG
GGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATT
GTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTC
CTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTG
TTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTC
ATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCC
AGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCT
GTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGG
AG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Multiple exon skipping

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL
YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC
PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF
RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG
RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL
QAGKGRH*EAGPGQAAQAASGESRDSAGRESGE
GGRGGHLAGVLGGGAVTHACRLLLTGGAASFWD
WTALLRSHRPHPFPRPTITTASGRHPHLPPVPSP
PHYTAHQPLQGSHGLSGEQFSPGLSSQLPPPTH
AYTHALLDKANIPLSRTLHLLRPHSLGGGDIAL
WAFGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQ
APSHPGTPYSTALAAAGVANGGGCWPPGFPQPN
CLCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAG
GVSPLLPLHCGHSPLALRLGSEYILW*WRCSHLL
SR*GPRALWPPPEVGWGCSPNPTLLPPLSHFPL
LRWGTNNKELTLPAPNPPPAPPCPPRFWFHFSS
VHKLPLDSCVVFCSMFHSSTSVIAAATSAKCSSS
LPPVLPTIPSPKILFVGKRGWGMAGWVTDYPSP
REGGALPLGCCSRVSKGAQIDHKGCRGHLLPLF
CWGGVAMICPSLGLPLWFPICSYLNKKNILFWTG

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY
IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF
VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS
DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA
GGRARASQPSLFHLLLREGKMSESSSKSSQPLA
SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQEK
EEEEGISQESSEEEQ*PMRAACSSLEEQLPSGT
GQLCSAPTAPTPSPGPPSPPPLAATPIFHLCPH
HHTTQHTSRCRAPMG*VGSSFPLASVPSSPRPPT
HTHMPSWTRLTSHLAAPCTCCVPTPLVVGTLLS
GLLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGP
KPHLILARPTPLPWQQQVWPMEGGAGPQDSPSQT
VFVTTWGSLFILPQLP*SPY*VGQPPSVTGRQE
G*VPYSLFTVATAPLPSAWDLSTYCGDGDAVTY
CPGEAQEPCGRHLRWAGAAPLTLLCFRHSAISPS
SDGAPITRSSPCPLPTPLLLLPAPQGSGSIFPL
FTNYLWTVVLFFVQCSILRHPSLLLLPAPNVHP
HCLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVP
GKVGPCP*DAAAE*ARGPKSTIKGVGATSSPCS
VGEG*P*FVPAWGSLSGFLFAVT*IKKISFSGL
E

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI
SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL
SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF
QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP
AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP
PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRR
RRKRRASRRSPRRRSSDPCVPPAPHWRSSFLLGL
DSFAPLPPPPPLPQAHHHHRLWPPPPSSTCALT
TTLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHP
RIHTCPPGQG*HPT*PHPAPAASPLPWWWGHCSL
GFWFGGALSAPSLFPLASHSGAWEGSPGLKRGP
SPISSWHALLHCPGSSRCGQWRGVLAPRIPPAK
LSLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRR
GESPTPSSLWPQPPCPPPGI*VHIVVMEMQSLI
VQVRPKSPVAAT*GGLGLLP*PYFASATQPFPP
PQMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLC
SQTTSGQLCCFLFNVPFFDIRHCCCYQRQMFIL
IASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQS
QGRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVL
LGRGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Sequence HMGA1.vhAug05

CTGGAAAAATGTGTAGCCTAGGCCTCCGGCGTCTCATCCATCTGTAGCCTGGGGATAAGGACTAGGATGAAGATGTGGAGGACTGTCCTTTTTAAGCCTG
TAATACAGGGTATTCCCAAATACAGGTTGCAGGCCCTCCTTCCAATGAGAAGTTTTAGGAAAACTCAAAATTGATGGGGTTAATTAAACCCCAACAGGCC
TCTAAGAAGAAGGAAGATGCTAGAAAATGACCAAGAAGGTTTTAGGATGAGCCCTAAACCTCATCCTAAAGGATGAGGGCCAGCCAGGGTCTGTTAGGCG
GTGCATTTCAGTTGGAGAATTCCCTACCCATCTGACAAAGTCCCATCTGACTCCAGTTGGGCAGAGGAGGGGTAAGGGGAAAGAGGAGGGTGGCCTTTGG
CTTTGGGCTCACTTTCCTCCCCCACCTCACCCACTAGCTTCAACCATCTCCTCCCTCCCTCAATTATACCAATCTGAATTCTCCAGGGATTTACCCTGGG
TGAGAACAGAGAAGAGAGCTGGTGGTGGGCACCACCCCTGGCTGTGTGATTGGCTCTTTTTGGTCCTGAGTCTTGCACCTTGCCAGATAGGCAATATTGT
CAGTGAGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGG
AAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGA
AGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCA
AGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCAC
TGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCAC
CCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCA
GTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTC
CCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGG
GTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCT
GGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGC
CCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTG
TGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGC
TTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCC
AAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCT
GCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAG
GCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTG
TAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAAT
AAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Multiple exon skipping

Frame 0

LEKCVA*ASGVSSICSLGIRTRMKMWRTVLFKP
VIQGIPKYRLQALLPMRSFRKTQN*WG*LNPNRP
LRRRKMLENDQEGFRMSPKPHPKG*GPARVC*A
VHFSWRIPYPSDKVPSDSSWAEEG*GERGGWPL
ALGSLSSPTSPTSFNHLLPPSIIPI*ILQGFTLG
ENREESWWWAPPLAV*LALFGPESCTLPDRQYC
Q*ASQPSLFHLLLREGKMSESSSKSSQPLASKQ
EKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSE
VPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRKP
RGRPKKLEKEEEEGISQESSEEEQ*PMRAACSS
LEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLAAT
PIFHLCPHHHTTQHTSRCRAPMG*VGSSFPLAS
VPSSPRPPTHTHMPSWTRLTSHLAAPCTCCVPT
PLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGLGG
FPWP*KGPKPHLILARPTPLPWQQQVWPMEGGA
GPQDSPSQTVFVTTWGSLFILPQLP*SPY*VGQ
PPSVTGRQEG*VPYSLFTVATAPLPSAWDLSTYC
GDGDAVTYCPGEAQEPCGRHLRWAGAAPLTLLC
FRHSAISPSSDGAPITRSSPCPLPTPLLLLPAP
QGSGSIFPLFTNYLWTVVLFFVQCSILRHPSLLL
LPAPNVHPHCLLFCPRSPPPRYSLWGRGAGAWQ
AG*PTTPVPGKVGPCP*DAAAE*ARGPKSTIKG
VGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT*I
KKISFSGLE

Frame 1

WKNV*PRPPASHPSVAWG*GLG*RCGGLSFLSL
*YRVFPNTGCRPSFQ*EVLGKLKIDGVN*TPTG
L*EEGRC*KMTKKVLG*ALNLILKDEGQPGSVRR
CISVGEFPTHLTKSHLTPVGQRRGKGKEEGGLW
LWAHFPPPPHPLASTISSLPQLYQSEFSRDLPW
VRTEKRAGGGHHPWLCDWLFLVLSLAPCQIGNIV
SEHPSHHSSTCSLEKGR*VSRARSPASPWPPSR
KRTALRSGAGAGRASSLR*VPGQRW*GVRRSPA
KCQHLRDLGADQREAKTRVLPRPGKPPQLQEGNQ
GADPKNWRRRKRRASRRSPRRRSSDPCVPPAPH
WRSSFLLGLDSFAPLPPPPPLPQAHHHHRLWPP
PPSSTCALTTTLHSTPAAAGLPWAEWGAVFPWPQ
FPAPPAHPRIHTCPPGQG*HPT*PHPAPAASPL
PWWWGHCSLGFWFGGALSAPSLFPLASHSGAWE
GSPGLKRGPSPISSWHALLHCPGSSRCGQWRGVL
APRIPPAKLSLSPRGAHFSSFPNFPSPRTRLDS
PLRLQEGRRGESPTPSSLWPQPPCPPPGI*VHI
VVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PYFA
SATQPFPPPQMGHQ*QGAHPARSQPPSCSSLPP
KVLVPFFLCSQTTSGQLCCFLFNVPFFDIRHCC
CYQRQMFILIASCSAHDPLPQDTLCGEEGLGHGR
LGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV
*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQLLE
*KKYPFLDW

Frame 2

GKMCSLGLRRLIHL*PGDKD*DEDVEDCPF*AC
NTGYSQIQVAGPPSNEKF*ENSKLMGLIKPQQA
SKKKEDARK*PRRF*DEP*TSS*RMRASQGLLG
GAFQLENSLPI*QSPI*LQLGRGGVRGKRRVAFG
FGLTFLPHLTH*LQPSPPSLNYTNLNSPGIYPG
*EQRRELVVGTTPGCVIGSFWS*VLHLAR*AIL
SVSIPAITLPPAP*RREDE*VELEVQPALGLQAG
KGRH*EAGPGQAAQAASGESRDSAGRESEGAQR
SANT*ETSGPTKGKQKQGCCQDPENHHNSRKET
KGQTQKTGEGGRGGHLAGVLGGGAVTHACRLLLT
GGAASFWDWTALLRSHRPHPFPRPTITTASGRH
PHLPPVPSPPHYTAHQPLQGSHGLSGEQFSPGL
SSQLPPPTHAYTHALLDKANIPLSRTLHLLRPHS
LGGGDIALWAFGLGAPSLLLHCSLWLPIVGPGR
VPLALKGAQAPSHPGTPYSTALAAAGVANGGGC
WPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGWTA
PFGYRKAGGVSPLLPLHCGHSPLALRLGSEYIL
W*WRCSHLLSR*GPRALWPPPEVGWGCSPNPTL
LPPLSHFPLLRWGTNNKELTLPAPNPPPAPPCPP
RFWFHFSSVHKLPLDSCVVFCSMFHSSTSVIAA
ATSAKCSSSLPPVLPTIPSPKILFVGKRGWGMA
GWVTDYPSPREGGALPLGCCSRVSKGAQIDHKGC
RGHLLPLFCWGGVAMICPSLGLPLWFPICSYLN
KKNILFWTG

Sequence HMGA1.vfAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGCATCCCAGCCATCACTCT
TCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGG
CCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGG
GGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGA
AGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGAC
TGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCAC
CACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCA
CGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTC
TGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCC
AAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAA
CTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGA
GGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTAT
TGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCT
CCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCT
GTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCT
CATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCC
CAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTC
TGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTG
GAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Multiple exon skipping

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSASQPSL
FHLLLREGKMSESSSKSSQPLASKQEKDGTEKRG
RGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPR
GRPKGSKNKGAAKTRKTTTTPGRKPRGRPKKLE
KEEEEGISQESSEEEQ*PMRAACSSLEEQLPSGT
GQLCSAPTAPTPSPGPPSPPPLAATPIFHLCPH
HHTTQHTSRCRAPMG*VGSSFPLASVPSSPRPP
THTHMPSWTRLTSHLAAPCTCCVPTPLVVGTLLS
GLLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGP
KPHLILARPTPLPWQQQVWPMEGGAGPQDSPSQ
TVFVTTWGSLFILPQLP*SPY*VGQPPSVTGRQE
G*VPYSLFTVATAPLPSAWDLSTYCGDGDAVTY
CPGEAQEPCGRHLRWAGAAPLTLLCFRHSAISP
SSDGAPITRSSPCPLPTPLLLLPAPQGSGSIFPL
FTNYLWTVVLFFVQCSILRHPSLLLLPAPNVHP
HCLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPV
PGKVGPCP*DAAAE*ARGPKSTIKGVGATSSPCS
VGEG*P*FVPAWGSLSGFLFAVT*IKKISFSGL
E

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPHPSHHS
STCSLEKGR*VSRARSPASPWPPSRKRTALRSG
AGAGRASSLR*VPGQRW*GVRRSPAKCQHLRDLG
ADQREAKTRVLPRPGKPPQLQEGNQGADPKNWR
RRKRRASRRSPRRRSSDPCVPPAPHWRSSFLLG
LDSFAPLPPPPPLPQAHHHHRLWPPPPSSTCALT
TTLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHP
RIHTCPPGQG*HPT*PHPAPAASPLPWWWGHCS
LGFWFGGALSAPSLFPLASHSGAWEGSPGLKRGP
SPISSWHALLHCPGSSRCGQWRGVLAPRIPPAK
LSLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGR
RGESPTPSSLWPQPPCPPPGI*VHIVVMEMQSLI
VQVRPKSPVAAT*GGLGLLP*PYFASATQPFPP
PQMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFL
CSQTTSGQLCCFLFNVPFFDIRHCCCYQRQMFIL
IASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQS
QGRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPV
LLGRGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRIPAITL
PPAP*RREDE*VELEVQPALGLQAGKGRH*EAG
PGQAAQAASGESRDSAGRESEGAQRSANT*ETS
GPTKGKQKQGCCQDPENHHNSRKETKGQTQKTGE
GGRGGHLAGVLGGGAVTHACRLLLTGGAASFWD
WTALLRSHRPHPFPRPTITTASGRHPHLPPVPS
PPHYTAHQPLQGSHGLSGEQFSPGLSSQLPPPTH
AYTHALLDKANIPLSRTLHLLRPHSLGGGDIAL
WAFGLGAPSLLLHCSLWLPIVGPGRVPLALKGA
QAPSHPGTPYSTALAAAGVANGGGCWPPGFPQPN
CLCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAG
GVSPLLPLHCGHSPLALRLGSEYILW*WRCSHL
LSR*GPRALWPPPEVGWGCSPNPTLLPPLSHFPL
LRWGTNNKELTLPAPNPPPAPPCPPRFWFHFSS
VHKLPLDSCVVFCSMFHSSTSVIAAATSAKCSS
SLPPVLPTIPSPKILFVGKRGWGMAGWVTDYPSP
REGGALPLGCCSRVSKGAQIDHKGCRGHLLPLF
CWGGVAMICPSLGLPLWFPICSYLNKKNILFWT
G

Sequence HMGA1.vcAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCCATCCCAGCCATCACTCTTCCACCTGCTCCTT
AGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGC
GCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGG
AAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAG
GGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCT
CCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACA
GCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATG
CCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTT
GGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCAT
CCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACC
ACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCT
ACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGC
CCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCA
CCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACC
TCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTT
CTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGG
CCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGT
AGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Multiple exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAAIPAITLPPAP
*RREDE*VELEVQPALGLQAGKGRH*EAGPGQAA
QAASGESRDSAGRESEGAQRSANT*ETSGPTKG
KQKQGCCQDPENHHNSRKETKGQTQKTGEGGRG
GHLAGVLGGGAVTHACRLLLTGGAASFWDWTALL
RSHRPHPFPRPTITTASGRHPHLPPVPSPPHYT
AHQPLQGSHGLSGEQFSPGLSSQLPPPTHAYTH
ALLDKANIPLSRTLHLLRPHSLGGGDIALWAFGL
GAPSLLLHCSLWLPIVGPGRVPLALKGAQAPSH
PGTPYSTALAAAGVANGGGCWPPGFPQPNCLCH
HVGLTFHPSPTSLVPVLGWTAPFGYRKAGGVSPL
LPLHCGHSPLALRLGSEYILW*WRCSHLLSR*G
PRALWPPPEVGWGCSPNPTLLPPLSHFPLLRWG
TNNKELTLPAPNPPPAPPCPPRFWFHFSSVHKLP
LDSCVVFCSMFHSSTSVIAAATSAKCSSSLPPV
LPTIPSPKILFVGKRGWGMAGWVTDYPSPREGG
ALPLGCCSRVSKGAQIDHKGCRGHLLPLFCWGGV
AMICPSLGLPLWFPICSYLNKKNILFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLPSQPSLFHLLL
REGKMSESSSKSSQPLASKQEKDGTEKRGRGRP
RKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKG
SKNKGAAKTRKTTTTPGRKPRGRPKKLEKEEEE
GISQESSEEEQ*PMRAACSSLEEQLPSGTGQLC
SAPTAPTPSPGPPSPPPLAATPIFHLCPHHHTTQ
HTSRCRAPMG*VGSSFPLASVPSSPRPPTHTHM
PSWTRLTSHLAAPCTCCVPTPLVVGTLLSGLLV
WGRPLCSFTVPSGFP*WGLGGFPWP*KGPKPHLI
LARPTPLPWQQQVWPMEGGAGPQDSPSQTVFVT
TWGSLFILPQLP*SPY*VGQPPSVTGRQEG*VP
YSLFTVATAPLPSAWDLSTYCGDGDAVTYCPGEA
QEPCGRHLRWAGAAPLTLLCFRHSAISPSSDGA
PITRSSPCPLPTPLLLLPAPQGSGSIFPLFTNY
LWTVVLFFVQCSILRHPSLLLLPAPNVHPHCLLF
CPRSPPPRYSLWGRGAGAWQAG*PTTPVPGKVG
PCP*DAAAE*ARGPKSTIKGVGATSSPCSVGEG
*P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCHPSHHSSTCSL
EKGR*VSRARSPASPWPPSRKRTALRSGAGAGR
ASSLR*VPGQRW*GVRRSPAKCQHLRDLGADQR
EAKTRVLPRPGKPPQLQEGNQGADPKNWRRRKRR
ASRRSPRRRSSDPCVPPAPHWRSSFLLGLDSFA
PLPPPPPLPQAHHHHRLWPPPPSSTCALTTTLH
STPAAAGLPWAEWGAVFPWPQFPAPPAHPRIHTC
PPGQG*HPT*PHPAPAASPLPWWWGHCSLGFWF
GGALSAPSLFPLASHSGAWEGSPGLKRGPSPIS
SWHALLHCPGSSRCGQWRGVLAPRIPPAKLSLSP
RGAHFSSFPNFPSPRTRLDSPLRLQEGRRGESP
TPSSLWPQPPCPPPGI*VHIVVMEMQSLIVQVR
PKSPVAAT*GGLGLLP*PYFASATQPFPPPQMGH
Q*QGAHPARSQPPSCSSLPPKVLVPFFLCSQTT
SGQLCCFLFNVPFFDIRHCCCYQRQMFILIASC
SAHDPLPQDTLCGEEGLGHGRLGDRLPQSQGRWG
PAPRMLQQSEQGGPNRP*RV*GPPPPPVLLGRG
SHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Sequence HMGA1.tAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG
CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA
GGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCA
CCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACC
CATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCA
TCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGG
GGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACC
CTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGC
TTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGG
TGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTA
GTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCC
GCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGG
CTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCC
CCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATT
CTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGG
GAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGG
GCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGT
TTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Multiple exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS
QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV
GSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKT
TTTPGRKPRGRPKKLEKEEEEGISQESSEEEQ*P
MRAACSSLEEQLPSGTGQLCSAPTAPTPSPGPP
SPPPLAATPIFHLCPHHHTTQHTSRCRAPMG*V
GSSFPLASVPSSPRPPTHTHMPSWTRLTSHLAAP
CTCCVPTPLVVGTLLSGLLVWGRPLCSFTVPSG
FP*WGLGGFPWP*KGPKPHLILARPTPLPWQQQ
VWPMEGGAGPQDSPSQTVFVTTWGSLFILPQLP*
SPY*VGQPPSVTGRQEG*VPYSLFTVATAPLPS
AWDLSTYCGDGDAVTYCPGEAQEPCGRHLRWAG
AAPLTLLCFRHSAISPSSDGAPITRSSPCPLPTP
LLLLPAPQGSGSIFPLFTNYLWTVVLFFVQCSI
LRHPSLLLLPAPNVHPHCLLFCPRSPPPRYSLW
GRGAGAWQAG*PTTPVPGKVGPCP*DAAAE*ARG
PKSTIKGVGATSSPCSVGEG*P*FVPAWGSLSG
FLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP
ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW*
GVRRSPAKCQHLRDLGADQREAKTRVLPRPGKP
PQLQEGNQGADPKNWRRRKRRASRRSPRRRSSD
PCVPPAPHWRSSFLLGLDSFAPLPPPPPLPQAHH
HHRLWPPPPSSTCALTTTLHSTPAAAGLPWAEW
GAVFPWPQFPAPPAHPRIHTCPPGQG*HPT*PH
PAPAASPLPWWWGHCSLGFWFGGALSAPSLFPLA
SHSGAWEGSPGLKRGPSPISSWHALLHCPGSSR
CGQWRGVLAPRIPPAKLSLSPRGAHFSSFPNFP
SPRTRLDSPLRLQEGRRGESPTPSSLWPQPPCPP
PGI*VHIVVMEMQSLIVQVRPKSPVAAT*GGLG
LLP*PYFASATQPFPPPQMGHQ*QGAHPARSQP
PSCSSLPPKVLVPFFLCSQTTSGQLCCFLFNVPF
FDIRHCCCYQRQMFILIASCSAHDPLPQDTLCG
EEGLGHGRLGDRLPQSQGRWGPAPRMLQQSEQG
GPNRP*RV*GPPPPPVLLGRGSHDLSQPGAPSLV
SYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ
PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG
RESEGAQRSANT*ETSGPTKGKQKQGCCQDPENH
HNSRKETKGQTQKTGEGGRGGHLAGVLGGGAVT
HACRLLLTGGAASFWDWTALLRSHRPHPFPRPT
ITTASGRHPHLPPVPSPPHYTAHQPLQGSHGLSG
EQFSPGLSSQLPPPTHAYTHALLDKANIPLSRT
LHLLRPHSLGGGDIALWAFGLGAPSLLLHCSLW
LPIVGPGRVPLALKGAQAPSHPGTPYSTALAAAG
VANGGGCWPPGFPQPNCLCHHVGLTFHPSPTSL
VPVLGWTAPFGYRKAGGVSPLLPLHCGHSPLAL
RLGSEYILW*WRCSHLLSR*GPRALWPPPEVGWG
CSPNPTLLPPLSHFPLLRWGTNNKELTLPAPNP
PPAPPCPPRFWFHFSSVHKLPLDSCVVFCSMFH
SSTSVIAAATSAKCSSSLPPVLPTIPSPKILFVG
KRGWGMAGWVTDYPSPREGGALPLGCCSRVSKG
AQIDHKGCRGHLLPLFCWGGVAMICPSLGLPLW
FPICSYLNKKNILFWTG

Sequence HMGA1.kAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGATCCGCATTTGCTACCAG
CGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATC
CCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGC
ACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACAC
CTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACC
CAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAG
CTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCC
ACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTC
CCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTG
GGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGC
CTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGA
TTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTT
ACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAG
ATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTC
AGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGT
TCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGC
CAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACC
GACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACC
TCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATAT
CCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Multiple exon skipping

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSDPHLLP
AAAAAEPGRSSAPSTAAPGNPERAPQAGGRARAS
QPSLFHLLLREGKMSESSSKSSQPLASKQEKDG
TEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPT
PKRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRP
KKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQ
LPSGTGQLCSAPTAPTPSPGPPSPPPLAATPIF
HLCPHHHTTQHTSRCRAPMG*VGSSFPLASVPSS
PRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVV
GTLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPW
P*KGPKPHLILARPTPLPWQQQVWPMEGGAGPQD
SPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSV
TGRQEG*VPYSLFTVATAPLPSAWDLSTYCGDG
DAVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRHS
AISPSSDGAPITRSSPCPLPTPLLLLPAPQGSG
SIFPLFTNYLWTVVLFFVQCSILRHPSLLLLPA
PNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*P
TTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGAT
SSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKI
SFSGLE

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPIRICYQ
RRPRRSQAGPQRPAPPLPATRSAHRRPAAELAH
PSHHSSTCSLEKGR*VSRARSPASPWPPSRKRTA
LRSGAGAGRASSLR*VPGQRW*GVRRSPAKCQH
LRDLGADQREAKTRVLPRPGKPPQLQEGNQGAD
PKNWRRRKRRASRRSPRRRSSDPCVPPAPHWRSS
FLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSS
TCALTTTLHSTPAAAGLPWAEWGAVFPWPQFPA
PPAHPRIHTCPPGQG*HPT*PHPAPAASPLPWWW
GHCSLGFWFGGALSAPSLFPLASHSGAWEGSPG
LKRGPSPISSWHALLHCPGSSRCGQWRGVLAPR
IPPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLRL
QEGRRGESPTPSSLWPQPPCPPPGI*VHIVVME
MQSLIVQVRPKSPVAAT*GGLGLLP*PYFASAT
QPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVLV
PFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQR
QMFILIASCSAHDPLPQDTLCGEEGLGHGRLGD
RLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GPP
PPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKY
PFLDW

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRSAFATS
GGRGGARPVLSAQHRRSRQPGARTAGRRPSSRI
PAITLPPAP*RREDE*VELEVQPALGLQAGKGR
H*EAGPGQAAQAASGESRDSAGRESEGAQRSANT
*ETSGPTKGKQKQGCCQDPENHHNSRKETKGQT
QKTGEGGRGGHLAGVLGGGAVTHACRLLLTGGA
ASFWDWTALLRSHRPHPFPRPTITTASGRHPHLP
PVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQL
PPPTHAYTHALLDKANIPLSRTLHLLRPHSLGG
GDIALWAFGLGAPSLLLHCSLWLPIVGPGRVPLA
LKGAQAPSHPGTPYSTALAAAGVANGGGCWPPG
FPQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFG
YRKAGGVSPLLPLHCGHSPLALRLGSEYILW*WR
CSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPL
SHFPLLRWGTNNKELTLPAPNPPPAPPCPPRFW
FHFSSVHKLPLDSCVVFCSMFHSSTSVIAAATSA
KCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVT
DYPSPREGGALPLGCCSRVSKGAQIDHKGCRGH
LLPLFCWGGVAMICPSLGLPLWFPICSYLNKKNI
LFWTG

Sequence HMGA1.fAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG
GGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAA
GACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAG
GAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTT
CCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCC
CATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCC
CACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCA
CTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCC
CTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTT
CCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAG
CCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCT
GAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGC
CCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTG
TTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAG
ATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCA
GAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGG
GCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Multiple exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP
GTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAK
TRKTTTTPGRKPRGRPKKLEKEEEEGISQESSE
EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTP
SPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAP
MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS
HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSF
TVPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP
WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL
PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVAT
APLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL
RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC
PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFF
VQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR
YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA
E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAW
GSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP
GQRW*GVRRSPAKCQHLRDLGADQREAKTRVLP
RPGKPPQLQEGNQGADPKNWRRRKRRASRRSPRR
RSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL
PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGL
PWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP
T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS
LFPLASHSGAWEGSPGLKRGPSPISSWHALLHC
PGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF
PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ
PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT
*GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA
RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL
FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQ
DTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ
SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG
APSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRESEGAQRSANT*ETSGPTKGKQKQGCCQ
DPENHHNSRKETKGQTQKTGEGGRGGHLAGVLG
GGAVTHACRLLLTGGAASFWDWTALLRSHRPHPF
PRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS
HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANI
PLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH
CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA
LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHP
SPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS
PLALRLGSEYILW*WRCSHLLSR*GPRALWPPP
EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTL
PAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC
SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK
ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCS
RVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG
LPLWFPICSYLNKKNILFWTG

Sequence HMGA1.eAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCG
GCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAG
CTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGG
ACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGA
CCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGA
GGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCC
CCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCA
TGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCA
CTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACT
GTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCT
GGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCC
CCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCC
CCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGA
GGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCC
GCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTT
CAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGAT
ACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGA
GTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGC
TCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Multiple exon skipping

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRSAFATSGGRGGARPVLSAQHRRSR
QPGARTAGRRPSSRIPAITLPPAP*RREDE*VE
LEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRESEGAQRSANT*ETSGPTKGKQKQGCCQD
PENHHNSRKETKGQTQKTGEGGRGGHLAGVLGG
GAVTHACRLLLTGGAASFWDWTALLRSHRPHPF
PRPTITTASGRHPHLPPVPSPPHYTAHQPLQGSH
GLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP
LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH
CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTAL
AAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS
PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS
PLALRLGSEYILW*WRCSHLLSR*GPRALWPPPE
VGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP
APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC
SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPKI
LFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR
VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG
LPLWFPICSYLNKKNILFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEDPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSESS
SKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPG
TALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAK
TRKTTTTPGRKPRGRPKKLEKEEEEGISQESSEE
EQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS
PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAP
MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSH
LAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT
VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP
WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFILP
QLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA
PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL
RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPCP
LPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV
QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR
YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE
*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG
SLSGFLFAVT*IKKISFSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKIRICYQRRPRRSQAGPQRPAPPLP
ATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VPG
QRW*GVRRSPAKCQHLRDLGADQREAKTRVLPR
PGKPPQLQEGNQGADPKNWRRRKRRASRRSPRR
RSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLP
QAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP
WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP
T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPSL
FPLASHSGAWEGSPGLKRGPSPISSWHALLHCP
GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF
PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQP
PCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*
GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA
RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLF
NVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD
TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ
SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGA
PSLVSYLQLLE*KKYPFLDW

Sequence HMGA1.cAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT
ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG
TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC
AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC
GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT
CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGA
GCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGA
AGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCT
GCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCT
GGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCC
CTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCG
TCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGG
CCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAG
GGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGG
TTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAG
TACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACC
CTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCC
CTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTC
ATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGG
GCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCA
TAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGT
TACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Multiple exon skipping

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL
YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC
PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF
RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG
RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL
QAGKGRH*EAGPGQAAQAASGESRDSAGRESEG
AQRSANT*ETSGPTKGKQKQGCCQDPENHHNSR
KETKGQTQKTGEGGRGGHLAGVLGGGAVTHACRL
LLTGGAASFWDWTALLRSHRPHPFPRPTITTAS
GRHPHLPPVPSPPHYTAHQPLQGSHGLSGEQFS
PGLSSQLPPPTHAYTHALLDKANIPLSRTLHLLR
PHSLGGGDIALWAFGLGAPSLLLHCSLWLPIVG
PGRVPLALKGAQAPSHPGTPYSTALAAAGVANG
GGCWPPGFPQPNCLCHHVGLTFHPSPTSLVPVLG
WTAPFGYRKAGGVSPLLPLHCGHSPLALRLGSE
YILW*WRCSHLLSR*GPRALWPPPEVGWGCSPN
PTLLPPLSHFPLLRWGTNNKELTLPAPNPPPAPP
CPPRFWFHFSSVHKLPLDSCVVFCSMFHSSTSV
IAAATSAKCSSSLPPVLPTIPSPKILFVGKRGW
GMAGWVTDYPSPREGGALPLGCCSRVSKGAQIDH
KGCRGHLLPLFCWGGVAMICPSLGLPLWFPICS
YLNKKNILFWTG

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY
IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF
VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS
DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA
GGRARASQPSLFHLLLREGKMSESSSKSSQPLA
SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKE
PSEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPG
RKPRGRPKKLEKEEEEGISQESSEEEQ*PMRAA
CSSLEEQLPSGTGQLCSAPTAPTPSPGPPSPPPL
AATPIFHLCPHHHTTQHTSRCRAPMG*VGSSFP
LASVPSSPRPPTHTHMPSWTRLTSHLAAPCTCC
VPTPLVVGTLLSGLLVWGRPLCSFTVPSGFP*WG
LGGFPWP*KGPKPHLILARPTPLPWQQQVWPME
GGAGPQDSPSQTVFVTTWGSLFILPQLP*SPY*
VGQPPSVTGRQEG*VPYSLFTVATAPLPSAWDLS
TYCGDGDAVTYCPGEAQEPCGRHLRWAGAAPLT
LLCFRHSAISPSSDGAPITRSSPCPLPTPLLLL
PAPQGSGSIFPLFTNYLWTVVLFFVQCSILRHPS
LLLLPAPNVHPHCLLFCPRSPPPRYSLWGRGAG
AWQAG*PTTPVPGKVGPCP*DAAAE*ARGPKST
IKGVGATSSPCSVGEG*P*FVPAWGSLSGFLFAV
T*IKKISFSGLE

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI
SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL
SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF
QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP
AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP
PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRR
SPAKCQHLRDLGADQREAKTRVLPRPGKPPQLQE
GNQGADPKNWRRRKRRASRRSPRRRSSDPCVPP
APHWRSSFLLGLDSFAPLPPPPPLPQAHHHHRL
WPPPPSSTCALTTTLHSTPAAAGLPWAEWGAVFP
WPQFPAPPAHPRIHTCPPGQG*HPT*PHPAPAA
SPLPWWWGHCSLGFWFGGALSAPSLFPLASHSG
AWEGSPGLKRGPSPISSWHALLHCPGSSRCGQWR
GVLAPRIPPAKLSLSPRGAHFSSFPNFPSPRTR
LDSPLRLQEGRRGESPTPSSLWPQPPCPPPGI*
VHIVVMEMQSLIVQVRPKSPVAAT*GGLGLLP*P
YFASATQPFPPPQMGHQ*QGAHPARSQPPSCSS
LPPKVLVPFFLCSQTTSGQLCCFLFNVPFFDIR
HCCCYQRQMFILIASCSAHDPLPQDTLCGEEGLG
HGRLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP
*RV*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQ
LLE*KKYPFLDW

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