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Sequence HMGA1.vnAug05
GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTATATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG
TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC
AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC
GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT
CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGGAGAA
GGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACT
GGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACC
ACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCAC
GCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCT
GGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCA
AGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAAC
TGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAG
GGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATT
GTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTC
CTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTG
TTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTC
ATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCC
AGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCT
GTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGG
AG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Multiple exon skipping
Frame 0
APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFLYL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC
PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF
RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG
RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL
QAGKGRH*EAGPGQAAQAASGESRDSAGRESGE
GGRGGHLAGVLGGGAVTHACRLLLTGGAASFWD
WTALLRSHRPHPFPRPTITTASGRHPHLPPVPSP
PHYTAHQPLQGSHGLSGEQFSPGLSSQLPPPTH
AYTHALLDKANIPLSRTLHLLRPHSLGGGDIAL
WAFGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQ
APSHPGTPYSTALAAAGVANGGGCWPPGFPQPN
CLCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAG
GVSPLLPLHCGHSPLALRLGSEYILW*WRCSHLL
SR*GPRALWPPPEVGWGCSPNPTLLPPLSHFPL
LRWGTNNKELTLPAPNPPPAPPCPPRFWFHFSS
VHKLPLDSCVVFCSMFHSSTSVIAAATSAKCSSS
LPPVLPTIPSPKILFVGKRGWGMAGWVTDYPSP
REGGALPLGCCSRVSKGAQIDHKGCRGHLLPLF
CWGGVAMICPSLGLPLWFPICSYLNKKNILFWTG
Frame 1
LL*LGLRAGAISGAGAAPRRREFAAAPVASFFYIYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF
VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS
DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA
GGRARASQPSLFHLLLREGKMSESSSKSSQPLA
SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQEK
EEEEGISQESSEEEQ*PMRAACSSLEEQLPSGT
GQLCSAPTAPTPSPGPPSPPPLAATPIFHLCPH
HHTTQHTSRCRAPMG*VGSSFPLASVPSSPRPPT
HTHMPSWTRLTSHLAAPCTCCVPTPLVVGTLLS
GLLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGP
KPHLILARPTPLPWQQQVWPMEGGAGPQDSPSQT
VFVTTWGSLFILPQLP*SPY*VGQPPSVTGRQE
G*VPYSLFTVATAPLPSAWDLSTYCGDGDAVTY
CPGEAQEPCGRHLRWAGAAPLTLLCFRHSAISPS
SDGAPITRSSPCPLPTPLLLLPAPQGSGSIFPL
FTNYLWTVVLFFVQCSILRHPSLLLLPAPNVHP
HCLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVP
GKVGPCP*DAAAE*ARGPKSTIKGVGATSSPCS
VGEG*P*FVPAWGSLSGFLFAVT*IKKISFSGL
E
Frame 2
SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFISII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL
SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF
QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP
AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP
PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRR
RRKRRASRRSPRRRSSDPCVPPAPHWRSSFLLGL
DSFAPLPPPPPLPQAHHHHRLWPPPPSSTCALT
TTLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHP
RIHTCPPGQG*HPT*PHPAPAASPLPWWWGHCSL
GFWFGGALSAPSLFPLASHSGAWEGSPGLKRGP
SPISSWHALLHCPGSSRCGQWRGVLAPRIPPAK
LSLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRR
GESPTPSSLWPQPPCPPPGI*VHIVVMEMQSLI
VQVRPKSPVAAT*GGLGLLP*PYFASATQPFPP
PQMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLC
SQTTSGQLCCFLFNVPFFDIRHCCCYQRQMFIL
IASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQS
QGRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVL
LGRGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW
Sequence HMGA1.uAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG
CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA
GGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCA
CAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCAT
GCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCA
CCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGA
GCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTG
CACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTC
CCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGT
GGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTC
CCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCC
TGGGATCTGAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGC
CACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTT
CTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACC
AGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGG
TGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGG
CCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAA
AATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Multiple exon skipping
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGAISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS
QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV
GSQEPSEVPTPKRPRGRPKGSKNKGAAKTRKTT
TTPGRKPRGRPKKLEKEEEEGISQESSEEEQ*PM
RAACSSLEEQLPSGTGQLCSAPTAPTPSPGPPS
PPPLAATPIFHLCPHHHTTQHTSRCRAPMG*VG
SSFPLASVPSSPRPPTHTHMPSWTRLTSHLAAPC
TCCVPTPLVVGTLLSGLLVWGRPLCSFTVPSGF
P*WGLGGFPWP*KGPKPHLILARPTPLPWQQQV
WPMEGGAGPQDSPSQTVFVTTWGSLFILPQLP*S
PY*VGQPPSVTGRQEG*VPYSLFTVATAPLPSA
WDLSHLLSR*GPRALWPPPEVGWGCSPNPTLLP
PLSHFPLLRWGTNNKELTLPAPNPPPAPPCPPRF
WFHFSSVHKLPLDSCVVFCSMFHSSTSVIAAAT
SAKCSSSLPPVLPTIPSPKILFVGKRGWGMAGW
VTDYPSPREGGALPLGCCSRVSKGAQIDHKGCRG
HLLPLFCWGGVAMICPSLGLPLWFPICSYLNKK
NILFWTG
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGLFLALARLQEGHPSHHSSTCSLEKGR*VSRARSP
ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW*
GVRSPAKCQHLRDLGADQREAKTRVLPRPGKPP
QLQEGNQGADPKNWRRRKRRASRRSPRRRSSDP
CVPPAPHWRSSFLLGLDSFAPLPPPPPLPQAHHH
HRLWPPPPSSTCALTTTLHSTPAAAGLPWAEWG
AVFPWPQFPAPPAHPRIHTCPPGQG*HPT*PHP
APAASPLPWWWGHCSLGFWFGGALSAPSLFPLAS
HSGAWEGSPGLKRGPSPISSWHALLHCPGSSRC
GQWRGVLAPRIPPAKLSLSPRGAHFSSFPNFPS
PRTRLDSPLRLQEGRRGESPTPSSLWPQPPCPPP
GI*VTYCPGEAQEPCGRHLRWAGAAPLTLLCFR
HSAISPSSDGAPITRSSPCPLPTPLLLLPAPQG
SGSIFPLFTNYLWTVVLFFVQCSILRHPSLLLLP
APNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG
*PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVG
ATSSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKK
ISFSGLE
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGYFWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ
PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG
RESGAQRSANT*ETSGPTKGKQKQGCCQDPENHH
NSRKETKGQTQKTGEGGRGGHLAGVLGGGAVTH
ACRLLLTGGAASFWDWTALLRSHRPHPFPRPTI
TTASGRHPHLPPVPSPPHYTAHQPLQGSHGLSGE
QFSPGLSSQLPPPTHAYTHALLDKANIPLSRTL
HLLRPHSLGGGDIALWAFGLGAPSLLLHCSLWL
PIVGPGRVPLALKGAQAPSHPGTPYSTALAAAGV
ANGGGCWPPGFPQPNCLCHHVGLTFHPSPTSLV
PVLGWTAPFGYRKAGGVSPLLPLHCGHSPLALR
LGSESLIVQVRPKSPVAAT*GGLGLLP*PYFASA
TQPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKV
LVPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCY
QRQMFILIASCSAHDPLPQDTLCGEEGLGHGRLG
DRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*G
PPPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*K
KYPFLDW
Sequence HMGA1.gAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG
GGACAGCGCTGGTAGGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGAC
CCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAG
GAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCC
CAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCAT
GGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCAC
TTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTG
TTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTG
GCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCC
CAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCC
CCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAG
GTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCG
CTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTC
AATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATA
CTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAG
TGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCT
CCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Multiple exon skipping
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGAISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP
GTALVGSQEPSEVPTPKRPRGRPKGSKNKGAAKT
RKTTTTPGRKPRGRPKKLEKEEEEGISQESSEE
EQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS
PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAPM
G*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSH
LAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT
VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLPW
QQQVWPMEGGAGPQDSPSQTVFVTTWGSLFILP
QLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA
PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLR
WAGAAPLTLLCFRHSAISPSSDGAPITRSSPCP
LPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV
QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPRY
SLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE
*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG
SLSGFLFAVT*IKKISFSGLE
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGLFLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP
GQRW*GVRSPAKCQHLRDLGADQREAKTRVLPR
PGKPPQLQEGNQGADPKNWRRRKRRASRRSPRRR
SSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLP
QAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP
WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT
*PHPAPAASPLPWWWGHCSLGFWFGGALSAPSL
FPLASHSGAWEGSPGLKRGPSPISSWHALLHCP
GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFP
NFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQP
PCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*
GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPAR
SQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLF
NVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD
TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQS
EQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGA
PSLVSYLQLLE*KKYPFLDW
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGYFWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRESGAQRSANT*ETSGPTKGKQKQGCCQD
PENHHNSRKETKGQTQKTGEGGRGGHLAGVLGG
GAVTHACRLLLTGGAASFWDWTALLRSHRPHPFP
RPTITTASGRHPHLPPVPSPPHYTAHQPLQGSH
GLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP
LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLHC
SLWLPIVGPGRVPLALKGAQAPSHPGTPYSTAL
AAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS
PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSP
LALRLGSEYILW*WRCSHLLSR*GPRALWPPPE
VGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP
APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCS
MFHSSTSVIAAATSAKCSSSLPPVLPTIPSPKI
LFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR
VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLGL
PLWFPICSYLNKKNILFWTG
Sequence HMGA1.dAug05
GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTATATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG
TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC
AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC
GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT
CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGGAGCC
CAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGG
AAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCT
CCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGC
CGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTG
GCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCC
CCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCT
GGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGG
GGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTG
GACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTAC
ATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTA
CTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTG
CCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATT
GCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCA
TGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAA
AGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTAC
TTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Multiple exon skipping
Frame 0
APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFLYL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC
PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF
RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG
RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL
QAGKGRH*EAGPGQAAQAASGESRDSAGRESGA
QRSANT*ETSGPTKGKQKQGCCQDPENHHNSRK
ETKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL
LTGGAASFWDWTALLRSHRPHPFPRPTITTASG
RHPHLPPVPSPPHYTAHQPLQGSHGLSGEQFSP
GLSSQLPPPTHAYTHALLDKANIPLSRTLHLLRP
HSLGGGDIALWAFGLGAPSLLLHCSLWLPIVGP
GRVPLALKGAQAPSHPGTPYSTALAAAGVANGG
GCWPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGW
TAPFGYRKAGGVSPLLPLHCGHSPLALRLGSEY
ILW*WRCSHLLSR*GPRALWPPPEVGWGCSPNP
TLLPPLSHFPLLRWGTNNKELTLPAPNPPPAPPC
PPRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVI
AAATSAKCSSSLPPVLPTIPSPKILFVGKRGWG
MAGWVTDYPSPREGGALPLGCCSRVSKGAQIDHK
GCRGHLLPLFCWGGVAMICPSLGLPLWFPICSY
LNKKNILFWTG
Frame 1
LL*LGLRAGAISGAGAAPRRREFAAAPVASFFYIYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF
VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS
DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA
GGRARASQPSLFHLLLREGKMSESSSKSSQPLA
SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQEP
SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGR
KPRGRPKKLEKEEEEGISQESSEEEQ*PMRAAC
SSLEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLA
ATPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPL
ASVPSSPRPPTHTHMPSWTRLTSHLAAPCTCCV
PTPLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGL
GGFPWP*KGPKPHLILARPTPLPWQQQVWPMEG
GAGPQDSPSQTVFVTTWGSLFILPQLP*SPY*V
GQPPSVTGRQEG*VPYSLFTVATAPLPSAWDLST
YCGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTL
LCFRHSAISPSSDGAPITRSSPCPLPTPLLLLP
APQGSGSIFPLFTNYLWTVVLFFVQCSILRHPSL
LLLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGA
WQAG*PTTPVPGKVGPCP*DAAAE*ARGPKSTI
KGVGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT
*IKKISFSGLE
Frame 2
SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFISII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL
SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF
QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP
AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP
PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRS
PAKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG
NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPA
PHWRSSFLLGLDSFAPLPPPPPLPQAHHHHRLW
PPPPSSTCALTTTLHSTPAAAGLPWAEWGAVFPW
PQFPAPPAHPRIHTCPPGQG*HPT*PHPAPAAS
PLPWWWGHCSLGFWFGGALSAPSLFPLASHSGA
WEGSPGLKRGPSPISSWHALLHCPGSSRCGQWRG
VLAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRL
DSPLRLQEGRRGESPTPSSLWPQPPCPPPGI*V
HIVVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PY
FASATQPFPPPQMGHQ*QGAHPARSQPPSCSSL
PPKVLVPFFLCSQTTSGQLCCFLFNVPFFDIRH
CCCYQRQMFILIASCSAHDPLPQDTLCGEEGLGH
GRLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*
RV*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQL
LE*KKYPFLDW
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