Sequence HMGA1.qAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA
GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGA
GGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCT
TCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTC
CCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATC
CCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTC
ACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGC
CCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCT
TCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACA
GCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACC
TGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTG
CCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTT
GTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAA
GATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGC
AGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGG
GGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP
SEVPTPKRPRGRPKGSKNKGEKEEEEGISQESSE
EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTP
SPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRA
PMG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS
HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSF
TVPSGFP*WGLGGFPWP*KGPKPHLILARPTPL
PWQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL
PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVAT
APLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRH
LRWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC
PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFF
VQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPP
RYSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA
E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAW
GSLSGFLFAVT*IKKISFSGLE
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP
AKCQHLRDLGADQREAKTRVRRRKRRASRRSPR
RRSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL
PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGL
PWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*H
PT*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS
LFPLASHSGAWEGSPGLKRGPSPISSWHALLHC
PGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSS
FPNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ
PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT
*GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHP
ARSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL
FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQ
DTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQ
QSEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG
APSLVSYLQLLE*KKYPFLDW
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ
RSANT*ETSGPTKGKQKQG*EGGRGGHLAGVLG
GGAVTHACRLLLTGGAASFWDWTALLRSHRPHP
FPRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS
HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANI
PLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLL
HCSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA
LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHP
SPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGH
SPLALRLGSEYILW*WRCSHLLSR*GPRALWPPP
EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTL
PAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVF
CSMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK
ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCS
RVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSL
GLPLWFPICSYLNKKNILFWTG
Sequence HMGA1.vmAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG
CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA
GGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCA
TCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGC
TCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCAC
ACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCT
CCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGG
GCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTG
GCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGT
GGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTC
CCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAA
GAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAA
TAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTG
GACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGC
CCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCT
GCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCC
ATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS
QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV
GSQKEPSEVPTPKRPRGRPKGSKNKGEKEEEEG
ISQESSEEEQ*PMRAACSSLEEQLPSGTGQLCSA
PTAPTPSPGPPSPPPLAATPIFHLCPHHHTTQH
TSRCRAPMG*VGSSFPLASVPSSPRPPTHTHMP
SWTRLTSHLAAPCTCCVPTPLVVGTLLSGLLVWG
RPLCSFTVPSGFP*WGLGGFPWP*KGPKPHLIL
ARPTPLPWQQQVWPMEGGAGPQDSPSQTVFVTT
WGSLFILPQLP*SPY*VGQPPSVTGRQEG*VPYS
LFTVATAPLPSAWDLSTYCGDGDAVTYCPGEAQ
EPCGRHLRWAGAAPLTLLCFRHSAISPSSDGAP
ITRSSPCPLPTPLLLLPAPQGSGSIFPLFTNYLW
TVVLFFVQCSILRHPSLLLLPAPNVHPHCLLFC
PRSPPPRYSLWGRGAGAWQAG*PTTPVPGKVGP
CP*DAAAE*ARGPKSTIKGVGATSSPCSVGEG*P
*FVPAWGSLSGFLFAVT*IKKISFSGLE
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP
ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW*
GVRRSPAKCQHLRDLGADQREAKTRVRRRKRRA
SRRSPRRRSSDPCVPPAPHWRSSFLLGLDSFAP
LPPPPPLPQAHHHHRLWPPPPSSTCALTTTLHST
PAAAGLPWAEWGAVFPWPQFPAPPAHPRIHTCP
PGQG*HPT*PHPAPAASPLPWWWGHCSLGFWFG
GALSAPSLFPLASHSGAWEGSPGLKRGPSPISSW
HALLHCPGSSRCGQWRGVLAPRIPPAKLSLSPR
GAHFSSFPNFPSPRTRLDSPLRLQEGRRGESPT
PSSLWPQPPCPPPGI*VHIVVMEMQSLIVQVRPK
SPVAAT*GGLGLLP*PYFASATQPFPPPQMGHQ
*QGAHPARSQPPSCSSLPPKVLVPFFLCSQTTS
GQLCCFLFNVPFFDIRHCCCYQRQMFILIASCSA
HDPLPQDTLCGEEGLGHGRLGDRLPQSQGRWGP
APRMLQQSEQGGPNRP*RV*GPPPPPVLLGRGS
HDLSQPGAPSLVSYLQLLE*KKYPFLDW
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ
PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG
RESEGAQRSANT*ETSGPTKGKQKQG*EGGRGGH
LAGVLGGGAVTHACRLLLTGGAASFWDWTALLR
SHRPHPFPRPTITTASGRHPHLPPVPSPPHYTA
HQPLQGSHGLSGEQFSPGLSSQLPPPTHAYTHAL
LDKANIPLSRTLHLLRPHSLGGGDIALWAFGLG
APSLLLHCSLWLPIVGPGRVPLALKGAQAPSHP
GTPYSTALAAAGVANGGGCWPPGFPQPNCLCHHV
GLTFHPSPTSLVPVLGWTAPFGYRKAGGVSPLL
PLHCGHSPLALRLGSEYILW*WRCSHLLSR*GP
RALWPPPEVGWGCSPNPTLLPPLSHFPLLRWGTN
NKELTLPAPNPPPAPPCPPRFWFHFSSVHKLPL
DSCVVFCSMFHSSTSVIAAATSAKCSSSLPPVL
PTIPSPKILFVGKRGWGMAGWVTDYPSPREGGAL
PLGCCSRVSKGAQIDHKGCRGHLLPLFCWGGVA
MICPSLGLPLWFPICSYLNKKNILFWTG
Sequence HMGA1.nAug05
GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT
ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG
TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC
AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC
GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT
CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGA
GCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCC
TCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCA
CCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAG
GGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTA
ACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCT
CCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCC
ACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTC
ATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGG
CCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCG
CCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCA
CCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGT
TTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCC
CCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCT
GCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAG
CCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL
YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC
PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF
RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG
RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL
QAGKGRH*EAGPGQAAQAASGESRDSAGRESEG
AQRSANT*ETSGPTKGKQKQG*EGGRGGHLAGV
LGGGAVTHACRLLLTGGAASFWDWTALLRSHRPH
PFPRPTITTASGRHPHLPPVPSPPHYTAHQPLQ
GSHGLSGEQFSPGLSSQLPPPTHAYTHALLDKA
NIPLSRTLHLLRPHSLGGGDIALWAFGLGAPSLL
LHCSLWLPIVGPGRVPLALKGAQAPSHPGTPYS
TALAAAGVANGGGCWPPGFPQPNCLCHHVGLTF
HPSPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCG
HSPLALRLGSEYILW*WRCSHLLSR*GPRALWP
PPEVGWGCSPNPTLLPPLSHFPLLRWGTNNKEL
TLPAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVV
FCSMFHSSTSVIAAATSAKCSSSLPPVLPTIPS
PKILFVGKRGWGMAGWVTDYPSPREGGALPLGC
CSRVSKGAQIDHKGCRGHLLPLFCWGGVAMICPS
LGLPLWFPICSYLNKKNILFWTG
Frame 1
LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY
IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF
VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS
DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA
GGRARASQPSLFHLLLREGKMSESSSKSSQPLA
SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKE
PSEVPTPKRPRGRPKGSKNKGEKEEEEGISQES
SEEEQ*PMRAACSSLEEQLPSGTGQLCSAPTAP
TPSPGPPSPPPLAATPIFHLCPHHHTTQHTSRCR
APMG*VGSSFPLASVPSSPRPPTHTHMPSWTRL
TSHLAAPCTCCVPTPLVVGTLLSGLLVWGRPLC
SFTVPSGFP*WGLGGFPWP*KGPKPHLILARPTP
LPWQQQVWPMEGGAGPQDSPSQTVFVTTWGSLF
ILPQLP*SPY*VGQPPSVTGRQEG*VPYSLFTV
ATAPLPSAWDLSTYCGDGDAVTYCPGEAQEPCGR
HLRWAGAAPLTLLCFRHSAISPSSDGAPITRSS
PCPLPTPLLLLPAPQGSGSIFPLFTNYLWTVVL
FFVQCSILRHPSLLLLPAPNVHPHCLLFCPRSPP
PRYSLWGRGAGAWQAG*PTTPVPGKVGPCP*DA
AAE*ARGPKSTIKGVGATSSPCSVGEG*P*FVP
AWGSLSGFLFAVT*IKKISFSGLE
Frame 2
SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI
SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL
SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF
QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP
AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP
PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRR
SPAKCQHLRDLGADQREAKTRVRRRKRRASRRSP
RRRSSDPCVPPAPHWRSSFLLGLDSFAPLPPPP
PLPQAHHHHRLWPPPPSSTCALTTTLHSTPAAA
GLPWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*
HPT*PHPAPAASPLPWWWGHCSLGFWFGGALSA
PSLFPLASHSGAWEGSPGLKRGPSPISSWHALL
HCPGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFS
SFPNFPSPRTRLDSPLRLQEGRRGESPTPSSLW
PQPPCPPPGI*VHIVVMEMQSLIVQVRPKSPVA
AT*GGLGLLP*PYFASATQPFPPPQMGHQ*QGAH
PARSQPPSCSSLPPKVLVPFFLCSQTTSGQLCC
FLFNVPFFDIRHCCCYQRQMFILIASCSAHDPL
PQDTLCGEEGLGHGRLGDRLPQSQGRWGPAPRML
QQSEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQ
PGAPSLVSYLQLLE*KKYPFLDW
Sequence HMGA1.mAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG
GGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGA
GGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGA
CAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACC
ACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCA
TACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGG
CTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGC
CCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGT
CTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGG
TGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTC
CAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTC
AGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTC
ACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATT
GCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGG
GAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTT
GGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP
GTALVGSQKEPSEVPTPKRPRGRPKGSKNKGEKE
EEEGISQESSEEEQ*PMRAACSSLEEQLPSGTG
QLCSAPTAPTPSPGPPSPPPLAATPIFHLCPHH
HTTQHTSRCRAPMG*VGSSFPLASVPSSPRPPTH
THMPSWTRLTSHLAAPCTCCVPTPLVVGTLLSG
LLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGPK
PHLILARPTPLPWQQQVWPMEGGAGPQDSPSQTV
FVTTWGSLFILPQLP*SPY*VGQPPSVTGRQEG
*VPYSLFTVATAPLPSAWDLSTYCGDGDAVTYC
PGEAQEPCGRHLRWAGAAPLTLLCFRHSAISPSS
DGAPITRSSPCPLPTPLLLLPAPQGSGSIFPLF
TNYLWTVVLFFVQCSILRHPSLLLLPAPNVHPH
CLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVPG
KVGPCP*DAAAE*ARGPKSTIKGVGATSSPCSV
GEG*P*FVPAWGSLSGFLFAVT*IKKISFSGLE
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP
GQRW*GVRRSPAKCQHLRDLGADQREAKTRVRR
RKRRASRRSPRRRSSDPCVPPAPHWRSSFLLGLD
SFAPLPPPPPLPQAHHHHRLWPPPPSSTCALTT
TLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHPR
IHTCPPGQG*HPT*PHPAPAASPLPWWWGHCSLG
FWFGGALSAPSLFPLASHSGAWEGSPGLKRGPS
PISSWHALLHCPGSSRCGQWRGVLAPRIPPAKL
SLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRRG
ESPTPSSLWPQPPCPPPGI*VHIVVMEMQSLIV
QVRPKSPVAAT*GGLGLLP*PYFASATQPFPPP
QMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLCS
QTTSGQLCCFLFNVPFFDIRHCCCYQRQMFILI
ASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQSQ
GRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVLL
GRGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRESEGAQRSANT*ETSGPTKGKQKQG*EG
GRGGHLAGVLGGGAVTHACRLLLTGGAASFWDW
TALLRSHRPHPFPRPTITTASGRHPHLPPVPSPP
HYTAHQPLQGSHGLSGEQFSPGLSSQLPPPTHA
YTHALLDKANIPLSRTLHLLRPHSLGGGDIALW
AFGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQA
PSHPGTPYSTALAAAGVANGGGCWPPGFPQPNC
LCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAGG
VSPLLPLHCGHSPLALRLGSEYILW*WRCSHLLS
R*GPRALWPPPEVGWGCSPNPTLLPPLSHFPLL
RWGTNNKELTLPAPNPPPAPPCPPRFWFHFSSV
HKLPLDSCVVFCSMFHSSTSVIAAATSAKCSSSL
PPVLPTIPSPKILFVGKRGWGMAGWVTDYPSPR
EGGALPLGCCSRVSKGAQIDHKGCRGHLLPLFC
WGGVAMICPSLGLPLWFPICSYLNKKNILFWTG
Sequence HMGA1.rAug05
GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT
ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG
TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC
AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC
GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT
CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGGAGCC
CAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCG
GAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCC
CTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGC
TCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACA
TCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCT
TCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACT
GCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATC
CTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCA
CAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCA
CCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCC
TGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTT
TTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCC
AAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCA
GCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCT
GGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL
YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC
PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF
RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG
RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL
QAGKGRH*EAGPGQAAQAASGESRDSAGRESGA
QRSANT*ETSGPTKGKQKQG*EGGRGGHLAGVL
GGGAVTHACRLLLTGGAASFWDWTALLRSHRPHP
FPRPTITTASGRHPHLPPVPSPPHYTAHQPLQG
SHGLSGEQFSPGLSSQLPPPTHAYTHALLDKAN
IPLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLL
HCSLWLPIVGPGRVPLALKGAQAPSHPGTPYST
ALAAAGVANGGGCWPPGFPQPNCLCHHVGLTFH
PSPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGH
SPLALRLGSEYILW*WRCSHLLSR*GPRALWPP
PEVGWGCSPNPTLLPPLSHFPLLRWGTNNKELT
LPAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVF
CSMFHSSTSVIAAATSAKCSSSLPPVLPTIPSP
KILFVGKRGWGMAGWVTDYPSPREGGALPLGCC
SRVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSL
GLPLWFPICSYLNKKNILFWTG
Frame 1
LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY
IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF
VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS
DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA
GGRARASQPSLFHLLLREGKMSESSSKSSQPLA
SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQEP
SEVPTPKRPRGRPKGSKNKGEKEEEEGISQESS
EEEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPT
PSPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRA
PMG*VGSSFPLASVPSSPRPPTHTHMPSWTRLT
SHLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCS
FTVPSGFP*WGLGGFPWP*KGPKPHLILARPTPL
PWQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFI
LPQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVA
TAPLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRH
LRWAGAAPLTLLCFRHSAISPSSDGAPITRSSP
CPLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLF
FVQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPP
RYSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAA
AE*ARGPKSTIKGVGATSSPCSVGEG*P*FVPA
WGSLSGFLFAVT*IKKISFSGLE
Frame 2
SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI
SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL
SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF
QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP
AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP
PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRS
PAKCQHLRDLGADQREAKTRVRRRKRRASRRSPR
RRSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPP
LPQAHHHHRLWPPPPSSTCALTTTLHSTPAAAG
LPWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*H
PT*PHPAPAASPLPWWWGHCSLGFWFGGALSAP
SLFPLASHSGAWEGSPGLKRGPSPISSWHALLH
CPGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSS
FPNFPSPRTRLDSPLRLQEGRRGESPTPSSLWP
QPPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAA
T*GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHP
ARSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCF
LFNVPFFDIRHCCCYQRQMFILIASCSAHDPLP
QDTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQ
QSEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQP
GAPSLVSYLQLLE*KKYPFLDW
Sequence HMGA1.oAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG
GGACAGCGCTGGTAGGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGA
AGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAG
CTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACA
CTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATAC
ACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTT
TTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCC
ATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTT
TGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGA
GTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAG
GTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGA
TGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACA
AACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCC
TCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAA
GGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGG
GAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP
GTALVGSQEPSEVPTPKRPRGRPKGSKNKGEKEE
EEGISQESSEEEQ*PMRAACSSLEEQLPSGTGQ
LCSAPTAPTPSPGPPSPPPLAATPIFHLCPHHH
TTQHTSRCRAPMG*VGSSFPLASVPSSPRPPTHT
HMPSWTRLTSHLAAPCTCCVPTPLVVGTLLSGL
LVWGRPLCSFTVPSGFP*WGLGGFPWP*KGPKP
HLILARPTPLPWQQQVWPMEGGAGPQDSPSQTVF
VTTWGSLFILPQLP*SPY*VGQPPSVTGRQEG*
VPYSLFTVATAPLPSAWDLSTYCGDGDAVTYCP
GEAQEPCGRHLRWAGAAPLTLLCFRHSAISPSSD
GAPITRSSPCPLPTPLLLLPAPQGSGSIFPLFT
NYLWTVVLFFVQCSILRHPSLLLLPAPNVHPHC
LLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVPGK
VGPCP*DAAAE*ARGPKSTIKGVGATSSPCSVG
EG*P*FVPAWGSLSGFLFAVT*IKKISFSGLE
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP
GQRW*GVRSPAKCQHLRDLGADQREAKTRVRRR
KRRASRRSPRRRSSDPCVPPAPHWRSSFLLGLDS
FAPLPPPPPLPQAHHHHRLWPPPPSSTCALTTT
LHSTPAAAGLPWAEWGAVFPWPQFPAPPAHPRI
HTCPPGQG*HPT*PHPAPAASPLPWWWGHCSLGF
WFGGALSAPSLFPLASHSGAWEGSPGLKRGPSP
ISSWHALLHCPGSSRCGQWRGVLAPRIPPAKLS
LSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRRGE
SPTPSSLWPQPPCPPPGI*VHIVVMEMQSLIVQ
VRPKSPVAAT*GGLGLLP*PYFASATQPFPPPQ
MGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLCSQ
TTSGQLCCFLFNVPFFDIRHCCCYQRQMFILIA
SCSAHDPLPQDTLCGEEGLGHGRLGDRLPQSQG
RWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVLLG
RGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRESGAQRSANT*ETSGPTKGKQKQG*EGG
RGGHLAGVLGGGAVTHACRLLLTGGAASFWDWT
ALLRSHRPHPFPRPTITTASGRHPHLPPVPSPPH
YTAHQPLQGSHGLSGEQFSPGLSSQLPPPTHAY
THALLDKANIPLSRTLHLLRPHSLGGGDIALWA
FGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQAP
SHPGTPYSTALAAAGVANGGGCWPPGFPQPNCL
CHHVGLTFHPSPTSLVPVLGWTAPFGYRKAGGV
SPLLPLHCGHSPLALRLGSEYILW*WRCSHLLSR
*GPRALWPPPEVGWGCSPNPTLLPPLSHFPLLR
WGTNNKELTLPAPNPPPAPPCPPRFWFHFSSVH
KLPLDSCVVFCSMFHSSTSVIAAATSAKCSSSLP
PVLPTIPSPKILFVGKRGWGMAGWVTDYPSPRE
GGALPLGCCSRVSKGAQIDHKGCRGHLLPLFCW
GGVAMICPSLGLPLWFPICSYLNKKNILFWTG
Sequence HMGA1.uAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG
CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA
GGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCA
CAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCAT
GCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCA
CCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGA
GCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTG
CACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTC
CCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGT
GGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTC
CCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCC
TGGGATCTGAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGC
CACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTT
CTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACC
AGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGG
TGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGG
CCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAA
AATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS
QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV
GSQEPSEVPTPKRPRGRPKGSKNKGAAKTRKTT
TTPGRKPRGRPKKLEKEEEEGISQESSEEEQ*PM
RAACSSLEEQLPSGTGQLCSAPTAPTPSPGPPS
PPPLAATPIFHLCPHHHTTQHTSRCRAPMG*VG
SSFPLASVPSSPRPPTHTHMPSWTRLTSHLAAPC
TCCVPTPLVVGTLLSGLLVWGRPLCSFTVPSGF
P*WGLGGFPWP*KGPKPHLILARPTPLPWQQQV
WPMEGGAGPQDSPSQTVFVTTWGSLFILPQLP*S
PY*VGQPPSVTGRQEG*VPYSLFTVATAPLPSA
WDLSHLLSR*GPRALWPPPEVGWGCSPNPTLLP
PLSHFPLLRWGTNNKELTLPAPNPPPAPPCPPRF
WFHFSSVHKLPLDSCVVFCSMFHSSTSVIAAAT
SAKCSSSLPPVLPTIPSPKILFVGKRGWGMAGW
VTDYPSPREGGALPLGCCSRVSKGAQIDHKGCRG
HLLPLFCWGGVAMICPSLGLPLWFPICSYLNKK
NILFWTG
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP
ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW*
GVRSPAKCQHLRDLGADQREAKTRVLPRPGKPP
QLQEGNQGADPKNWRRRKRRASRRSPRRRSSDP
CVPPAPHWRSSFLLGLDSFAPLPPPPPLPQAHHH
HRLWPPPPSSTCALTTTLHSTPAAAGLPWAEWG
AVFPWPQFPAPPAHPRIHTCPPGQG*HPT*PHP
APAASPLPWWWGHCSLGFWFGGALSAPSLFPLAS
HSGAWEGSPGLKRGPSPISSWHALLHCPGSSRC
GQWRGVLAPRIPPAKLSLSPRGAHFSSFPNFPS
PRTRLDSPLRLQEGRRGESPTPSSLWPQPPCPPP
GI*VTYCPGEAQEPCGRHLRWAGAAPLTLLCFR
HSAISPSSDGAPITRSSPCPLPTPLLLLPAPQG
SGSIFPLFTNYLWTVVLFFVQCSILRHPSLLLLP
APNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG
*PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVG
ATSSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKK
ISFSGLE
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ
PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG
RESGAQRSANT*ETSGPTKGKQKQGCCQDPENHH
NSRKETKGQTQKTGEGGRGGHLAGVLGGGAVTH
ACRLLLTGGAASFWDWTALLRSHRPHPFPRPTI
TTASGRHPHLPPVPSPPHYTAHQPLQGSHGLSGE
QFSPGLSSQLPPPTHAYTHALLDKANIPLSRTL
HLLRPHSLGGGDIALWAFGLGAPSLLLHCSLWL
PIVGPGRVPLALKGAQAPSHPGTPYSTALAAAGV
ANGGGCWPPGFPQPNCLCHHVGLTFHPSPTSLV
PVLGWTAPFGYRKAGGVSPLLPLHCGHSPLALR
LGSESLIVQVRPKSPVAAT*GGLGLLP*PYFASA
TQPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKV
LVPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCY
QRQMFILIASCSAHDPLPQDTLCGEEGLGHGRLG
DRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*G
PPPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*K
KYPFLDW
Sequence HMGA1.gAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG
GGACAGCGCTGGTAGGGAGTCAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGAC
CCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAG
GAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCC
CAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCAT
GGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCAC
TTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTG
TTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTG
GCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCC
CAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCC
CCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAG
GTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCG
CTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTC
AATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATA
CTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAG
TGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCT
CCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP
GTALVGSQEPSEVPTPKRPRGRPKGSKNKGAAKT
RKTTTTPGRKPRGRPKKLEKEEEEGISQESSEE
EQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS
PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAPM
G*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSH
LAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT
VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLPW
QQQVWPMEGGAGPQDSPSQTVFVTTWGSLFILP
QLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA
PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLR
WAGAAPLTLLCFRHSAISPSSDGAPITRSSPCP
LPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV
QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPRY
SLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE
*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG
SLSGFLFAVT*IKKISFSGLE
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP
GQRW*GVRSPAKCQHLRDLGADQREAKTRVLPR
PGKPPQLQEGNQGADPKNWRRRKRRASRRSPRRR
SSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLP
QAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP
WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT
*PHPAPAASPLPWWWGHCSLGFWFGGALSAPSL
FPLASHSGAWEGSPGLKRGPSPISSWHALLHCP
GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFP
NFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQP
PCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*
GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPAR
SQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLF
NVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD
TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQS
EQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGA
PSLVSYLQLLE*KKYPFLDW
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRESGAQRSANT*ETSGPTKGKQKQGCCQD
PENHHNSRKETKGQTQKTGEGGRGGHLAGVLGG
GAVTHACRLLLTGGAASFWDWTALLRSHRPHPFP
RPTITTASGRHPHLPPVPSPPHYTAHQPLQGSH
GLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP
LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLHC
SLWLPIVGPGRVPLALKGAQAPSHPGTPYSTAL
AAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS
PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSP
LALRLGSEYILW*WRCSHLLSR*GPRALWPPPE
VGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP
APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCS
MFHSSTSVIAAATSAKCSSSLPPVLPTIPSPKI
LFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR
VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLGL
PLWFPICSYLNKKNILFWTG
Sequence HMGA1.dAug05
GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT
ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG
TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC
AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC
GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT
CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGGAGCC
CAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGG
AAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCT
CCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGC
CGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTG
GCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCC
CCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCT
GGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGG
GGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTG
GACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTAC
ATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTA
CTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTG
CCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATT
GCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCA
TGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAA
AGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTAC
TTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL
YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC
PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF
RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG
RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL
QAGKGRH*EAGPGQAAQAASGESRDSAGRESGA
QRSANT*ETSGPTKGKQKQGCCQDPENHHNSRK
ETKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL
LTGGAASFWDWTALLRSHRPHPFPRPTITTASG
RHPHLPPVPSPPHYTAHQPLQGSHGLSGEQFSP
GLSSQLPPPTHAYTHALLDKANIPLSRTLHLLRP
HSLGGGDIALWAFGLGAPSLLLHCSLWLPIVGP
GRVPLALKGAQAPSHPGTPYSTALAAAGVANGG
GCWPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGW
TAPFGYRKAGGVSPLLPLHCGHSPLALRLGSEY
ILW*WRCSHLLSR*GPRALWPPPEVGWGCSPNP
TLLPPLSHFPLLRWGTNNKELTLPAPNPPPAPPC
PPRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVI
AAATSAKCSSSLPPVLPTIPSPKILFVGKRGWG
MAGWVTDYPSPREGGALPLGCCSRVSKGAQIDHK
GCRGHLLPLFCWGGVAMICPSLGLPLWFPICSY
LNKKNILFWTG
Frame 1
LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY
IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF
VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS
DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA
GGRARASQPSLFHLLLREGKMSESSSKSSQPLA
SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQEP
SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGR
KPRGRPKKLEKEEEEGISQESSEEEQ*PMRAAC
SSLEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLA
ATPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPL
ASVPSSPRPPTHTHMPSWTRLTSHLAAPCTCCV
PTPLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGL
GGFPWP*KGPKPHLILARPTPLPWQQQVWPMEG
GAGPQDSPSQTVFVTTWGSLFILPQLP*SPY*V
GQPPSVTGRQEG*VPYSLFTVATAPLPSAWDLST
YCGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTL
LCFRHSAISPSSDGAPITRSSPCPLPTPLLLLP
APQGSGSIFPLFTNYLWTVVLFFVQCSILRHPSL
LLLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGA
WQAG*PTTPVPGKVGPCP*DAAAE*ARGPKSTI
KGVGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT
*IKKISFSGLE
Frame 2
SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI
SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL
SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF
QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP
AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP
PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRS
PAKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG
NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPA
PHWRSSFLLGLDSFAPLPPPPPLPQAHHHHRLW
PPPPSSTCALTTTLHSTPAAAGLPWAEWGAVFPW
PQFPAPPAHPRIHTCPPGQG*HPT*PHPAPAAS
PLPWWWGHCSLGFWFGGALSAPSLFPLASHSGA
WEGSPGLKRGPSPISSWHALLHCPGSSRCGQWRG
VLAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRL
DSPLRLQEGRRGESPTPSSLWPQPPCPPPGI*V
HIVVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PY
FASATQPFPPPQMGHQ*QGAHPARSQPPSCSSL
PPKVLVPFFLCSQTTSGQLCCFLFNVPFFDIRH
CCCYQRQMFILIASCSAHDPLPQDTLCGEEGLGH
GRLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*
RV*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQL
LE*KKYPFLDW
Sequence HMGA1.vlAug05
GGGTCCCCAGCAGCAAGAGGTGGGGGGAGGCACCAGATGGTATGAGAGCTTCCAGGGAGACCCGCCAAGATCTCCAGGCAGGCCGGGCCAGGCTCTCTGG
GATTCCGGACTGGCTTCGCCCAACTGGAACCCCCTCGATGAGGGGGCCCCAGGCAGACCTTATATGAGCATCCCAGCCATCACTCTTCCACCTGCTCCTT
AGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGC
GCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAA
AACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAG
TGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCC
CACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTG
AGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCC
GCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCT
CTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCA
GCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTT
CCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGC
CCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGC
TGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCA
ACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTT
CCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTT
GTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCA
AGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCT
CTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
GSPAARGGGRHQMV*ELPGRPAKISRQAGPGSL
GFRTGFAQLEPPR*GGPRQTLYEHPSHHSSTCSL
EKGR*VSRARSPASPWPPSRKRTALRSGAGAGR
ASSLRRSPAKCQHLRDLGADQREAKTRVLPRPG
KPPQLQEGNQGADPKNWRRRKRRASRRSPRRRSS
DPCVPPAPHWRSSFLLGLDSFAPLPPPPPLPQA
HHHHRLWPPPPSSTCALTTTLHSTPAAAGLPWA
EWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT*P
HPAPAASPLPWWWGHCSLGFWFGGALSAPSLFP
LASHSGAWEGSPGLKRGPSPISSWHALLHCPGS
SRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFPNF
PSPRTRLDSPLRLQEGRRGESPTPSSLWPQPPC
PPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*GG
LGLLP*PYFASATQPFPPPQMGHQ*QGAHPARSQ
PPSCSSLPPKVLVPFFLCSQTTSGQLCCFLFNV
PFFDIRHCCCYQRQMFILIASCSAHDPLPQDTL
CGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQSEQ
GGPNRP*RV*GPPPPPVLLGRGSHDLSQPGAPS
LVSYLQLLE*KKYPFLDW
Frame 1
GPQQQEVGGGTRWYESFQGDPPRSPGRPGQALW
DSGLASPNWNPLDEGAPGRPYMSIPAITLPPAP
*RREDE*VELEVQPALGLQAGKGRH*EAGPGQAA
QAASEGAQRSANT*ETSGPTKGKQKQGCCQDPE
NHHNSRKETKGQTQKTGEGGRGGHLAGVLGGGA
VTHACRLLLTGGAASFWDWTALLRSHRPHPFPRP
TITTASGRHPHLPPVPSPPHYTAHQPLQGSHGL
SGEQFSPGLSSQLPPPTHAYTHALLDKANIPLS
RTLHLLRPHSLGGGDIALWAFGLGAPSLLLHCSL
WLPIVGPGRVPLALKGAQAPSHPGTPYSTALAA
AGVANGGGCWPPGFPQPNCLCHHVGLTFHPSPT
SLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSPLA
LRLGSEYILW*WRCSHLLSR*GPRALWPPPEVG
WGCSPNPTLLPPLSHFPLLRWGTNNKELTLPAP
NPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCSMF
HSSTSVIAAATSAKCSSSLPPVLPTIPSPKILF
VGKRGWGMAGWVTDYPSPREGGALPLGCCSRVS
KGAQIDHKGCRGHLLPLFCWGGVAMICPSLGLPL
WFPICSYLNKKNILFWTG
Frame 2
VPSSKRWGEAPDGMRASRETRQDLQAGRARLSG
IPDWLRPTGTPSMRGPQADLI*ASQPSLFHLLL
REGKMSESSSKSSQPLASKQEKDGTEKRGRGRP
RKQPPKEPSEVPTPKRPRGRPKGSKNKGAAKTRK
TTTTPGRKPRGRPKKLEKEEEEGISQESSEEEQ
*PMRAACSSLEEQLPSGTGQLCSAPTAPTPSPG
PPSPPPLAATPIFHLCPHHHTTQHTSRCRAPMG*
VGSSFPLASVPSSPRPPTHTHMPSWTRLTSHLA
APCTCCVPTPLVVGTLLSGLLVWGRPLCSFTVP
SGFP*WGLGGFPWP*KGPKPHLILARPTPLPWQQ
QVWPMEGGAGPQDSPSQTVFVTTWGSLFILPQL
P*SPY*VGQPPSVTGRQEG*VPYSLFTVATAPL
PSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLRWA
GAAPLTLLCFRHSAISPSSDGAPITRSSPCPLP
TPLLLLPAPQGSGSIFPLFTNYLWTVVLFFVQC
SILRHPSLLLLPAPNVHPHCLLFCPRSPPPRYSL
WGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE*A
RGPKSTIKGVGATSSPCSVGEG*P*FVPAWGSL
SGFLFAVT*IKKISFSGLE
Sequence HMGA1.vjAug05
GTTGGAACTAGTCCCTTTGACTCCCCGTTTCTATTTTATTTTCTTCTGGGGCTTTATTTTGCGGGGAGGGGACGGAGGGAGGGGGCTTTCTTCCTCTCCC
CCGCGCCCGCGCGCGCCAGGTTTCCTGCCCCAAGGCGGGCTTGGGTGCCCCCTCTGGCAGGAAGTGGGGCCGGGTGCACCCGCGTGGCGGTGCATGCGGT
CGGGGTGCGCTGCTTTCCCGCGCCTGCAGGAGCGGCCTGCGCCCCTCCCCCCGCGCGCCCTGGCCCCGGGTCCCGCCGGCCGGCCTGCAACGGCCGGGCG
CAGACACTTGCAGAGTCACCCTCGGGCCGGGTCTGGAGCCTGATGCCTCGGGGGTTTAGCCCTAGCCGCTAGGCGGCATCCCAGCCATCACTCTTCCACC
TGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGG
CAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAG
ACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGG
AGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTC
CCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCC
ATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCC
ACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCAC
TGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCC
TGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTC
CCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGC
CCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTG
AGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCC
CGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGT
TCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGA
TACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAG
AGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGG
CTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
VGTSPFDSPFLFYFLLGLYFAGRGRREGAFFLS
PAPARARFPAPRRAWVPPLAGSGAGCTRVAVHAV
GVRCFPAPAGAACAPPPARPGPGSRRPACNGRA
QTLAESPSGRVWSLMPRGFSPSR*AASQPSLFH
LLLREGKMSESSSKSSQPLASKQEKDGTEKRGRG
RPRKQPPKEPSEVPTPKRPRGRPKGSKNKGAAK
TRKTTTTPGRKPRGRPKKLEKEEEEGISQESSE
EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS
PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAP
MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS
HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT
VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP
WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL
PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA
PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL
RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC
PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV
QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR
YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA
E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG
SLSGFLFAVT*IKKISFSGLE
Frame 1
LELVPLTPRFYFIFFWGFILRGGDGGRGLSSSP
PRPRAPGFLPQGGLGCPLWQEVGPGAPAWRCMR
SGCAAFPRLQERPAPLPPRALAPGPAGRPATAGR
RHLQSHPRAGSGA*CLGGLALAARRHPSHHSST
CSLEKGR*VSRARSPASPWPPSRKRTALRSGAG
AGRASSLRRSPAKCQHLRDLGADQREAKTRVLPR
PGKPPQLQEGNQGADPKNWRRRKRRASRRSPRR
RSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL
PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP
WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP
T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS
LFPLASHSGAWEGSPGLKRGPSPISSWHALLHCP
GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF
PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ
PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*
GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA
RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL
FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD
TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ
SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG
APSLVSYLQLLE*KKYPFLDW
Frame 2
WN*SL*LPVSILFSSGALFCGEGTEGGGFLPLP
RARARQVSCPKAGLGAPSGRKWGRVHPRGGACG
RGALLSRACRSGLRPSPRAPWPRVPPAGLQRPG
ADTCRVTLGPGLEPDASGV*P*PLGGIPAITLPP
AP*RREDE*VELEVQPALGLQAGKGRH*EAGPG
QAAQAASEGAQRSANT*ETSGPTKGKQKQGCCQ
DPENHHNSRKETKGQTQKTGEGGRGGHLAGVLGG
GAVTHACRLLLTGGAASFWDWTALLRSHRPHPF
PRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS
HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP
LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH
CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA
LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS
PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS
PLALRLGSEYILW*WRCSHLLSR*GPRALWPPP
EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP
APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC
SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK
ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR
VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG
LPLWFPICSYLNKKNILFWTG
Sequence HMGA1.vgAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCC
AGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACC
TAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCC
AAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGC
TTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCA
CCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCC
CCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGG
GGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCC
TTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGAT
TCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTA
CAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGA
TGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCA
GCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTT
CCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCC
AAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCG
ACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCT
CCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATC
CTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRSIPAITLPPAP*RREDE*VELEVQP
ALGLQAGKGRH*EAGPGQAAQAASEGAQRSANT
*ETSGPTKGKQKQGCCQDPENHHNSRKETKGQT
QKTGEGGRGGHLAGVLGGGAVTHACRLLLTGGAA
SFWDWTALLRSHRPHPFPRPTITTASGRHPHLP
PVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQL
PPPTHAYTHALLDKANIPLSRTLHLLRPHSLGGG
DIALWAFGLGAPSLLLHCSLWLPIVGPGRVPLA
LKGAQAPSHPGTPYSTALAAAGVANGGGCWPPG
FPQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFGY
RKAGGVSPLLPLHCGHSPLALRLGSEYILW*WR
CSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPL
SHFPLLRWGTNNKELTLPAPNPPPAPPCPPRFWF
HFSSVHKLPLDSCVVFCSMFHSSTSVIAAATSA
KCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVT
DYPSPREGGALPLGCCSRVSKGAQIDHKGCRGHL
LPLFCWGGVAMICPSLGLPLWFPICSYLNKKNI
LFWTG
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEASQPSLFHLLLREGKMSESSSKSS
QPLASKQEKDGTEKRGRGRPRKQPPKEPSEVPTP
KRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRP
KKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQ
LPSGTGQLCSAPTAPTPSPGPPSPPPLAATPIFH
LCPHHHTTQHTSRCRAPMG*VGSSFPLASVPSS
PRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVV
GTLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPWP
*KGPKPHLILARPTPLPWQQQVWPMEGGAGPQD
SPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSV
TGRQEG*VPYSLFTVATAPLPSAWDLSTYCGDGD
AVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRHS
AISPSSDGAPITRSSPCPLPTPLLLLPAPQGSG
SIFPLFTNYLWTVVLFFVQCSILRHPSLLLLPAP
NVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*P
TTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGAT
SSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKIS
FSGLE
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKHPSHHSSTCSLEKGR*VSRARSPA
SPWPPSRKRTALRSGAGAGRASSLRRSPAKCQH
LRDLGADQREAKTRVLPRPGKPPQLQEGNQGADP
KNWRRRKRRASRRSPRRRSSDPCVPPAPHWRSS
FLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSS
TCALTTTLHSTPAAAGLPWAEWGAVFPWPQFPAP
PAHPRIHTCPPGQG*HPT*PHPAPAASPLPWWW
GHCSLGFWFGGALSAPSLFPLASHSGAWEGSPG
LKRGPSPISSWHALLHCPGSSRCGQWRGVLAPRI
PPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLRL
QEGRRGESPTPSSLWPQPPCPPPGI*VHIVVME
MQSLIVQVRPKSPVAAT*GGLGLLP*PYFASATQ
PFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVLV
PFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQR
QMFILIASCSAHDPLPQDTLCGEEGLGHGRLGDR
LPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GPP
PPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKY
PFLDW
Sequence HMGA1.vdAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG
CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACA
CCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGAC
CCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCA
GCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTC
CACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCT
CCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGT
GGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGG
CCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGG
ATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGT
TACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGA
GATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACT
CAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGG
TTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCG
CCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGAC
CGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCAC
CTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATA
TCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS
QPLASKQEKDGTEKRGRGRPRKQPPKEPSEVPT
PKRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGR
PKKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQ
LPSGTGQLCSAPTAPTPSPGPPSPPPLAATPIF
HLCPHHHTTQHTSRCRAPMG*VGSSFPLASVPS
SPRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVV
GTLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPW
P*KGPKPHLILARPTPLPWQQQVWPMEGGAGPQ
DSPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSV
TGRQEG*VPYSLFTVATAPLPSAWDLSTYCGDG
DAVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRH
SAISPSSDGAPITRSSPCPLPTPLLLLPAPQGSG
SIFPLFTNYLWTVVLFFVQCSILRHPSLLLLPA
PNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*
PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGAT
SSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKI
SFSGLE
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP
ASPWPPSRKRTALRSGAGAGRASSLRRSPAKCQH
LRDLGADQREAKTRVLPRPGKPPQLQEGNQGAD
PKNWRRRKRRASRRSPRRRSSDPCVPPAPHWRS
SFLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSS
TCALTTTLHSTPAAAGLPWAEWGAVFPWPQFPA
PPAHPRIHTCPPGQG*HPT*PHPAPAASPLPWW
WGHCSLGFWFGGALSAPSLFPLASHSGAWEGSPG
LKRGPSPISSWHALLHCPGSSRCGQWRGVLAPR
IPPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLR
LQEGRRGESPTPSSLWPQPPCPPPGI*VHIVVME
MQSLIVQVRPKSPVAAT*GGLGLLP*PYFASAT
QPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVL
VPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQR
QMFILIASCSAHDPLPQDTLCGEEGLGHGRLGD
RLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GP
PPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKY
PFLDW
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ
PALGLQAGKGRH*EAGPGQAAQAASEGAQRSAN
T*ETSGPTKGKQKQGCCQDPENHHNSRKETKGQT
QKTGEGGRGGHLAGVLGGGAVTHACRLLLTGGA
ASFWDWTALLRSHRPHPFPRPTITTASGRHPHL
PPVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQL
PPPTHAYTHALLDKANIPLSRTLHLLRPHSLGG
GDIALWAFGLGAPSLLLHCSLWLPIVGPGRVPL
ALKGAQAPSHPGTPYSTALAAAGVANGGGCWPPG
FPQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFG
YRKAGGVSPLLPLHCGHSPLALRLGSEYILW*W
RCSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPL
SHFPLLRWGTNNKELTLPAPNPPPAPPCPPRFW
FHFSSVHKLPLDSCVVFCSMFHSSTSVIAAATS
AKCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVT
DYPSPREGGALPLGCCSRVSKGAQIDHKGCRGH
LLPLFCWGGVAMICPSLGLPLWFPICSYLNKKN
ILFWTG
Sequence HMGA1.vbAug05
CAGGCTGGTCTCGAACTCCTGACCTCAAGCAGTCCTCCTGCTTTGGCATCCCAAAGTGCTGGGATTACAGCATCCCAGCCATCACTCTTCCACCTGCTCC
TTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCC
GCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGG
AAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGC
AGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGG
CCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGC
TGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAG
CCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCC
CTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAG
CAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAAC
TTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTT
GCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGG
GCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCC
CAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATG
TTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCT
TTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAG
CAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCT
CTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
QAGLELLTSSSPPALASQSAGITASQPSLFHLL
LREGKMSESSSKSSQPLASKQEKDGTEKRGRGRP
RKQPPKEPSEVPTPKRPRGRPKGSKNKGAAKTR
KTTTTPGRKPRGRPKKLEKEEEEGISQESSEEE
Q*PMRAACSSLEEQLPSGTGQLCSAPTAPTPSPG
PPSPPPLAATPIFHLCPHHHTTQHTSRCRAPMG
*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSHL
AAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFTVP
SGFP*WGLGGFPWP*KGPKPHLILARPTPLPWQ
QQVWPMEGGAGPQDSPSQTVFVTTWGSLFILPQ
LP*SPY*VGQPPSVTGRQEG*VPYSLFTVATAPL
PSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLRW
AGAAPLTLLCFRHSAISPSSDGAPITRSSPCPL
PTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFVQC
SILRHPSLLLLPAPNVHPHCLLFCPRSPPPRYS
LWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE*
ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWGSL
SGFLFAVT*IKKISFSGLE
Frame 1
RLVSNS*PQAVLLLWHPKVLGLQHPSHHSSTCS
LEKGR*VSRARSPASPWPPSRKRTALRSGAGAG
RASSLRRSPAKCQHLRDLGADQREAKTRVLPRPG
KPPQLQEGNQGADPKNWRRRKRRASRRSPRRRS
SDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLPQ
AHHHHRLWPPPPSSTCALTTTLHSTPAAAGLPWA
EWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT*
PHPAPAASPLPWWWGHCSLGFWFGGALSAPSLF
PLASHSGAWEGSPGLKRGPSPISSWHALLHCPGS
SRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFPN
FPSPRTRLDSPLRLQEGRRGESPTPSSLWPQPP
CPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*GG
LGLLP*PYFASATQPFPPPQMGHQ*QGAHPARS
QPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLFN
VPFFDIRHCCCYQRQMFILIASCSAHDPLPQDTL
CGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQSE
QGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGAP
SLVSYLQLLE*KKYPFLDW
Frame 2
GWSRTPDLKQSSCFGIPKCWDYSIPAITLPPAP
*RREDE*VELEVQPALGLQAGKGRH*EAGPGQA
AQAASEGAQRSANT*ETSGPTKGKQKQGCCQDP
ENHHNSRKETKGQTQKTGEGGRGGHLAGVLGGGA
VTHACRLLLTGGAASFWDWTALLRSHRPHPFPR
PTITTASGRHPHLPPVPSPPHYTAHQPLQGSHG
LSGEQFSPGLSSQLPPPTHAYTHALLDKANIPLS
RTLHLLRPHSLGGGDIALWAFGLGAPSLLLHCS
LWLPIVGPGRVPLALKGAQAPSHPGTPYSTALA
AAGVANGGGCWPPGFPQPNCLCHHVGLTFHPSPT
SLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSPL
ALRLGSEYILW*WRCSHLLSR*GPRALWPPPEV
GWGCSPNPTLLPPLSHFPLLRWGTNNKELTLPAP
NPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCSM
FHSSTSVIAAATSAKCSSSLPPVLPTIPSPKIL
FVGKRGWGMAGWVTDYPSPREGGALPLGCCSRVS
KGAQIDHKGCRGHLLPLFCWGGVAMICPSLGLP
LWFPICSYLNKKNILFWTG
Sequence HMGA1.pAug05
GAGCGCGAGTTGTGCACCCGGCGGAGCCCGGAGGAGCCCGGCGCACCTCGCGCGGCGGCCGCTCCCGCTGGAGCCGGAGCCCGAGCCCGAGCCCGGGCCC
GGGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAA
GGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAG
GGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGG
AGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTG
CTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACA
CAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACA
TGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGT
TTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTC
ATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCA
CCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCC
CTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAG
GCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGG
CACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTA
CCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTG
TTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGG
GGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGG
GTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
ERELCTRRSPEEPGAPRAAAAPAGAGARARARA
RASQPSLFHLLLREGKMSESSSKSSQPLASKQEK
DGTEKRGRGRPRKQPPKEPSEVPTPKRPRGRPK
GSKNKGAAKTRKTTTTPGRKPRGRPKKLEKEEE
EGISQESSEEEQ*PMRAACSSLEEQLPSGTGQLC
SAPTAPTPSPGPPSPPPLAATPIFHLCPHHHTT
QHTSRCRAPMG*VGSSFPLASVPSSPRPPTHTH
MPSWTRLTSHLAAPCTCCVPTPLVVGTLLSGLLV
WGRPLCSFTVPSGFP*WGLGGFPWP*KGPKPHL
ILARPTPLPWQQQVWPMEGGAGPQDSPSQTVFV
TTWGSLFILPQLP*SPY*VGQPPSVTGRQEG*VP
YSLFTVATAPLPSAWDLSTYCGDGDAVTYCPGE
AQEPCGRHLRWAGAAPLTLLCFRHSAISPSSDG
APITRSSPCPLPTPLLLLPAPQGSGSIFPLFTNY
LWTVVLFFVQCSILRHPSLLLLPAPNVHPHCLL
FCPRSPPPRYSLWGRGAGAWQAG*PTTPVPGKV
GPCP*DAAAE*ARGPKSTIKGVGATSSPCSVGEG
*P*FVPAWGSLSGFLFAVT*IKKISFSGLE
Frame 1
SASCAPGGARRSPAHLARRPLPLEPEPEPEPGP
GHPSHHSSTCSLEKGR*VSRARSPASPWPPSRK
RTALRSGAGAGRASSLRRSPAKCQHLRDLGADQR
EAKTRVLPRPGKPPQLQEGNQGADPKNWRRRKR
RASRRSPRRRSSDPCVPPAPHWRSSFLLGLDSF
APLPPPPPLPQAHHHHRLWPPPPSSTCALTTTLH
STPAAAGLPWAEWGAVFPWPQFPAPPAHPRIHT
CPPGQG*HPT*PHPAPAASPLPWWWGHCSLGFW
FGGALSAPSLFPLASHSGAWEGSPGLKRGPSPIS
SWHALLHCPGSSRCGQWRGVLAPRIPPAKLSLS
PRGAHFSSFPNFPSPRTRLDSPLRLQEGRRGES
PTPSSLWPQPPCPPPGI*VHIVVMEMQSLIVQVR
PKSPVAAT*GGLGLLP*PYFASATQPFPPPQMG
HQ*QGAHPARSQPPSCSSLPPKVLVPFFLCSQT
TSGQLCCFLFNVPFFDIRHCCCYQRQMFILIASC
SAHDPLPQDTLCGEEGLGHGRLGDRLPQSQGRW
GPAPRMLQQSEQGGPNRP*RV*GPPPPPVLLGR
GSHDLSQPGAPSLVSYLQLLE*KKYPFLDW
Frame 2
ARVVHPAEPGGARRTSRGGRSRWSRSPSPSPGP
GIPAITLPPAP*RREDE*VELEVQPALGLQAGK
GRH*EAGPGQAAQAASEGAQRSANT*ETSGPTK
GKQKQGCCQDPENHHNSRKETKGQTQKTGEGGRG
GHLAGVLGGGAVTHACRLLLTGGAASFWDWTAL
LRSHRPHPFPRPTITTASGRHPHLPPVPSPPHY
TAHQPLQGSHGLSGEQFSPGLSSQLPPPTHAYTH
ALLDKANIPLSRTLHLLRPHSLGGGDIALWAFG
LGAPSLLLHCSLWLPIVGPGRVPLALKGAQAPS
HPGTPYSTALAAAGVANGGGCWPPGFPQPNCLCH
HVGLTFHPSPTSLVPVLGWTAPFGYRKAGGVSP
LLPLHCGHSPLALRLGSEYILW*WRCSHLLSR*
GPRALWPPPEVGWGCSPNPTLLPPLSHFPLLRWG
TNNKELTLPAPNPPPAPPCPPRFWFHFSSVHKL
PLDSCVVFCSMFHSSTSVIAAATSAKCSSSLPP
VLPTIPSPKILFVGKRGWGMAGWVTDYPSPREGG
ALPLGCCSRVSKGAQIDHKGCRGHLLPLFCWGG
VAMICPSLGLPLWFPICSYLNKKNILFWTG
Sequence HMGA1.jAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA
GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAA
ACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCC
TCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATG
GAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCA
CTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCT
GGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAG
CGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTG
ACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCC
ACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAA
TATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP
SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRK
PRGRPKKLEKEEEEGISQESSEEEQ*PMRAACS
SLEEQLPSGTGQLCSAPTAPLPSAWDLSTYCGD
GDAVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRH
SAISPSSDGAPITRSSPCPLPTPLLLLPAPQGS
GSIFPLFTNYLWTVVLFFVQCSILRHPSLLLLP
APNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*
PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGA
TSSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKK
ISFSGLE
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP
AKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG
NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPAP
HWRSSFLLGLDSFAPLPQPPCPPPGI*VHIVVM
EMQSLIVQVRPKSPVAAT*GGLGLLP*PYFASA
TQPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVL
VPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQ
RQMFILIASCSAHDPLPQDTLCGEEGLGHGRLG
DRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GP
PPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KK
YPFLDW
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ
RSANT*ETSGPTKGKQKQGCCQDPENHHNSRKE
TKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL
LTGGAASFWDWTALLRSHSPLALRLGSEYILW*W
RCSHLLSR*GPRALWPPPEVGWGCSPNPTLLPP
LSHFPLLRWGTNNKELTLPAPNPPPAPPCPPRF
WFHFSSVHKLPLDSCVVFCSMFHSSTSVIAAATS
AKCSSSLPPVLPTIPSPKILFVGKRGWGMAGWV
TDYPSPREGGALPLGCCSRVSKGAQIDHKGCRG
HLLPLFCWGGVAMICPSLGLPLWFPICSYLNKKN
ILFWTG
Sequence HMGA1.iAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA
GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAA
ACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCC
TCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCG
CCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGC
CTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCC
ACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGG
GAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGG
TGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGA
CAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACAT
ATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACT
TTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCC
CCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGC
TGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATG
GCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAG
GGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTT
GAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP
SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRK
PRGRPKKLEKEEEEGISQESSEEEQ*PMRAACS
SLEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLA
ATPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPLA
SVPSSPRPPTHTHMPSWTRLTSHLAAPCTCCVP
TPLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGL
GGFPWP*KGPKPHLILARPTPLPWQQQVWPMEGG
AGPQDSPSQTVFVTTWGSLFILPQLP*SPY*VG
QPPSVTGRQEG*VPYSLFTVATAPLPSAWDLST
YCGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTLL
CFRHSAISPSSDGAPITRSSPCPLPTPLLLLPA
PQGSGSIFPLFTNYLWTVVLFFVQCSILRHPSL
LLLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGAW
QAG*PTTPVPGKVGPCP*DAAAE*ARGPKSTIK
GVGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT
*IKKISFSGLE
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP
AKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG
NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPAP
HWRSSFLLGLDSFAPLPPPPPLPQAHHHHRLWP
PPPSSTCALTTTLHSTPAAAGLPWAEWGAVFPW
PQFPAPPAHPRIHTCPPGQG*HPT*PHPAPAASP
LPWWWGHCSLGFWFGGALSAPSLFPLASHSGAW
EGSPGLKRGPSPISSWHALLHCPGSSRCGQWRG
VLAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRLD
SPLRLQEGRRGESPTPSSLWPQPPCPPPGI*VH
IVVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PY
FASATQPFPPPQMGHQ*QGAHPARSQPPSCSSLP
PKVLVPFFLCSQTTSGQLCCFLFNVPFFDIRHC
CCYQRQMFILIASCSAHDPLPQDTLCGEEGLGH
GRLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*R
V*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQLL
E*KKYPFLDW
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ
RSANT*ETSGPTKGKQKQGCCQDPENHHNSRKE
TKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL
LTGGAASFWDWTALLRSHRPHPFPRPTITTASGR
HPHLPPVPSPPHYTAHQPLQGSHGLSGEQFSPG
LSSQLPPPTHAYTHALLDKANIPLSRTLHLLRP
HSLGGGDIALWAFGLGAPSLLLHCSLWLPIVGPG
RVPLALKGAQAPSHPGTPYSTALAAAGVANGGG
CWPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGW
TAPFGYRKAGGVSPLLPLHCGHSPLALRLGSEYI
LW*WRCSHLLSR*GPRALWPPPEVGWGCSPNPT
LLPPLSHFPLLRWGTNNKELTLPAPNPPPAPPC
PPRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVIA
AATSAKCSSSLPPVLPTIPSPKILFVGKRGWGM
AGWVTDYPSPREGGALPLGCCSRVSKGAQIDHK
GCRGHLLPLFCWGGVAMICPSLGLPLWFPICSYL
NKKNILFWTG
Sequence HMGA1.hAug05
GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT
ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG
TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC
AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC
GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT
CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCG
GGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAG
AAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGA
CTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCA
CCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCC
ACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCT
CTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCC
CAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAA
ACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGG
AGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTA
TTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCC
TCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTC
TGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCC
TCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTC
CCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTT
CTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACT
GGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL
YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC
PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF
RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG
RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL
QAGKGRH*EAGPGQAAQAASEGAQRSANT*ETS
GPTKGKQKQGCCQDPENHHNSRKETKGQTQKTG
EGGRGGHLAGVLGGGAVTHACRLLLTGGAASFWD
WTALLRSHRPHPFPRPTITTASGRHPHLPPVPS
PPHYTAHQPLQGSHGLSGEQFSPGLSSQLPPPT
HAYTHALLDKANIPLSRTLHLLRPHSLGGGDIAL
WAFGLGAPSLLLHCSLWLPIVGPGRVPLALKGA
QAPSHPGTPYSTALAAAGVANGGGCWPPGFPQP
NCLCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAG
GVSPLLPLHCGHSPLALRLGSEYILW*WRCSHL
LSR*GPRALWPPPEVGWGCSPNPTLLPPLSHFP
LLRWGTNNKELTLPAPNPPPAPPCPPRFWFHFSS
VHKLPLDSCVVFCSMFHSSTSVIAAATSAKCSS
SLPPVLPTIPSPKILFVGKRGWGMAGWVTDYPS
PREGGALPLGCCSRVSKGAQIDHKGCRGHLLPLF
CWGGVAMICPSLGLPLWFPICSYLNKKNILFWT
G
Frame 1
LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY
IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF
VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS
DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA
GGRARASQPSLFHLLLREGKMSESSSKSSQPLA
SKQEKDGTEKRGRGRPRKQPPKEPSEVPTPKRPR
GRPKGSKNKGAAKTRKTTTTPGRKPRGRPKKLE
KEEEEGISQESSEEEQ*PMRAACSSLEEQLPSG
TGQLCSAPTAPTPSPGPPSPPPLAATPIFHLCPH
HHTTQHTSRCRAPMG*VGSSFPLASVPSSPRPP
THTHMPSWTRLTSHLAAPCTCCVPTPLVVGTLL
SGLLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGP
KPHLILARPTPLPWQQQVWPMEGGAGPQDSPSQ
TVFVTTWGSLFILPQLP*SPY*VGQPPSVTGRQ
EG*VPYSLFTVATAPLPSAWDLSTYCGDGDAVTY
CPGEAQEPCGRHLRWAGAAPLTLLCFRHSAISP
SSDGAPITRSSPCPLPTPLLLLPAPQGSGSIFP
LFTNYLWTVVLFFVQCSILRHPSLLLLPAPNVHP
HCLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPV
PGKVGPCP*DAAAE*ARGPKSTIKGVGATSSPC
SVGEG*P*FVPAWGSLSGFLFAVT*IKKISFSGL
E
Frame 2
SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI
SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL
SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF
QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP
AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP
PSRKRTALRSGAGAGRASSLRRSPAKCQHLRDL
GADQREAKTRVLPRPGKPPQLQEGNQGADPKNWR
RRKRRASRRSPRRRSSDPCVPPAPHWRSSFLLG
LDSFAPLPPPPPLPQAHHHHRLWPPPPSSTCAL
TTTLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHP
RIHTCPPGQG*HPT*PHPAPAASPLPWWWGHCS
LGFWFGGALSAPSLFPLASHSGAWEGSPGLKRG
PSPISSWHALLHCPGSSRCGQWRGVLAPRIPPAK
LSLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGR
RGESPTPSSLWPQPPCPPPGI*VHIVVMEMQSL
IVQVRPKSPVAAT*GGLGLLP*PYFASATQPFPP
PQMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFL
CSQTTSGQLCCFLFNVPFFDIRHCCCYQRQMFI
LIASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQS
QGRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPV
LLGRGSHDLSQPGAPSLVSYLQLLE*KKYPFLD
W
Sequence HMGA1.vhAug05
CTGGAAAAATGTGTAGCCTAGGCCTCCGGCGTCTCATCCATCTGTAGCCTGGGGATAAGGACTAGGATGAAGATGTGGAGGACTGTCCTTTTTAAGCCTG
TAATACAGGGTATTCCCAAATACAGGTTGCAGGCCCTCCTTCCAATGAGAAGTTTTAGGAAAACTCAAAATTGATGGGGTTAATTAAACCCCAACAGGCC
TCTAAGAAGAAGGAAGATGCTAGAAAATGACCAAGAAGGTTTTAGGATGAGCCCTAAACCTCATCCTAAAGGATGAGGGCCAGCCAGGGTCTGTTAGGCG
GTGCATTTCAGTTGGAGAATTCCCTACCCATCTGACAAAGTCCCATCTGACTCCAGTTGGGCAGAGGAGGGGTAAGGGGAAAGAGGAGGGTGGCCTTTGG
CTTTGGGCTCACTTTCCTCCCCCACCTCACCCACTAGCTTCAACCATCTCCTCCCTCCCTCAATTATACCAATCTGAATTCTCCAGGGATTTACCCTGGG
TGAGAACAGAGAAGAGAGCTGGTGGTGGGCACCACCCCTGGCTGTGTGATTGGCTCTTTTTGGTCCTGAGTCTTGCACCTTGCCAGATAGGCAATATTGT
CAGTGAGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGG
AAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGA
AGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCA
AGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCAC
TGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCAC
CCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCA
GTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTC
CCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGG
GTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCT
GGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGC
CCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTG
TGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGC
TTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCC
AAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCT
GCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAG
GCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTG
TAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAAT
AAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
LEKCVA*ASGVSSICSLGIRTRMKMWRTVLFKP
VIQGIPKYRLQALLPMRSFRKTQN*WG*LNPNRP
LRRRKMLENDQEGFRMSPKPHPKG*GPARVC*A
VHFSWRIPYPSDKVPSDSSWAEEG*GERGGWPL
ALGSLSSPTSPTSFNHLLPPSIIPI*ILQGFTLG
ENREESWWWAPPLAV*LALFGPESCTLPDRQYC
Q*ASQPSLFHLLLREGKMSESSSKSSQPLASKQ
EKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSE
VPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRKP
RGRPKKLEKEEEEGISQESSEEEQ*PMRAACSS
LEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLAAT
PIFHLCPHHHTTQHTSRCRAPMG*VGSSFPLAS
VPSSPRPPTHTHMPSWTRLTSHLAAPCTCCVPT
PLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGLGG
FPWP*KGPKPHLILARPTPLPWQQQVWPMEGGA
GPQDSPSQTVFVTTWGSLFILPQLP*SPY*VGQ
PPSVTGRQEG*VPYSLFTVATAPLPSAWDLSTYC
GDGDAVTYCPGEAQEPCGRHLRWAGAAPLTLLC
FRHSAISPSSDGAPITRSSPCPLPTPLLLLPAP
QGSGSIFPLFTNYLWTVVLFFVQCSILRHPSLLL
LPAPNVHPHCLLFCPRSPPPRYSLWGRGAGAWQ
AG*PTTPVPGKVGPCP*DAAAE*ARGPKSTIKG
VGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT*I
KKISFSGLE
Frame 1
WKNV*PRPPASHPSVAWG*GLG*RCGGLSFLSL
*YRVFPNTGCRPSFQ*EVLGKLKIDGVN*TPTG
L*EEGRC*KMTKKVLG*ALNLILKDEGQPGSVRR
CISVGEFPTHLTKSHLTPVGQRRGKGKEEGGLW
LWAHFPPPPHPLASTISSLPQLYQSEFSRDLPW
VRTEKRAGGGHHPWLCDWLFLVLSLAPCQIGNIV
SEHPSHHSSTCSLEKGR*VSRARSPASPWPPSR
KRTALRSGAGAGRASSLR*VPGQRW*GVRRSPA
KCQHLRDLGADQREAKTRVLPRPGKPPQLQEGNQ
GADPKNWRRRKRRASRRSPRRRSSDPCVPPAPH
WRSSFLLGLDSFAPLPPPPPLPQAHHHHRLWPP
PPSSTCALTTTLHSTPAAAGLPWAEWGAVFPWPQ
FPAPPAHPRIHTCPPGQG*HPT*PHPAPAASPL
PWWWGHCSLGFWFGGALSAPSLFPLASHSGAWE
GSPGLKRGPSPISSWHALLHCPGSSRCGQWRGVL
APRIPPAKLSLSPRGAHFSSFPNFPSPRTRLDS
PLRLQEGRRGESPTPSSLWPQPPCPPPGI*VHI
VVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PYFA
SATQPFPPPQMGHQ*QGAHPARSQPPSCSSLPP
KVLVPFFLCSQTTSGQLCCFLFNVPFFDIRHCC
CYQRQMFILIASCSAHDPLPQDTLCGEEGLGHGR
LGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV
*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQLLE
*KKYPFLDW
Frame 2
GKMCSLGLRRLIHL*PGDKD*DEDVEDCPF*AC
NTGYSQIQVAGPPSNEKF*ENSKLMGLIKPQQA
SKKKEDARK*PRRF*DEP*TSS*RMRASQGLLG
GAFQLENSLPI*QSPI*LQLGRGGVRGKRRVAFG
FGLTFLPHLTH*LQPSPPSLNYTNLNSPGIYPG
*EQRRELVVGTTPGCVIGSFWS*VLHLAR*AIL
SVSIPAITLPPAP*RREDE*VELEVQPALGLQAG
KGRH*EAGPGQAAQAASGESRDSAGRESEGAQR
SANT*ETSGPTKGKQKQGCCQDPENHHNSRKET
KGQTQKTGEGGRGGHLAGVLGGGAVTHACRLLLT
GGAASFWDWTALLRSHRPHPFPRPTITTASGRH
PHLPPVPSPPHYTAHQPLQGSHGLSGEQFSPGL
SSQLPPPTHAYTHALLDKANIPLSRTLHLLRPHS
LGGGDIALWAFGLGAPSLLLHCSLWLPIVGPGR
VPLALKGAQAPSHPGTPYSTALAAAGVANGGGC
WPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGWTA
PFGYRKAGGVSPLLPLHCGHSPLALRLGSEYIL
W*WRCSHLLSR*GPRALWPPPEVGWGCSPNPTL
LPPLSHFPLLRWGTNNKELTLPAPNPPPAPPCPP
RFWFHFSSVHKLPLDSCVVFCSMFHSSTSVIAA
ATSAKCSSSLPPVLPTIPSPKILFVGKRGWGMA
GWVTDYPSPREGGALPLGCCSRVSKGAQIDHKGC
RGHLLPLFCWGGVAMICPSLGLPLWFPICSYLN
KKNILFWTG
Sequence HMGA1.vfAug05
GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGCATCCCAGCCATCACTCT
TCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGG
CCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGG
GGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGA
AGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGAC
TGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCAC
CACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCA
CGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTC
TGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCC
AAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAA
CTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGA
GGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTAT
TGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCT
CCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCT
GTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCT
CATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCC
CAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTC
TGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTG
GAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
APLIGTPSRGYFWRWRGSKKA*VRGRSASQPSL
FHLLLREGKMSESSSKSSQPLASKQEKDGTEKRG
RGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPR
GRPKGSKNKGAAKTRKTTTTPGRKPRGRPKKLE
KEEEEGISQESSEEEQ*PMRAACSSLEEQLPSGT
GQLCSAPTAPTPSPGPPSPPPLAATPIFHLCPH
HHTTQHTSRCRAPMG*VGSSFPLASVPSSPRPP
THTHMPSWTRLTSHLAAPCTCCVPTPLVVGTLLS
GLLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGP
KPHLILARPTPLPWQQQVWPMEGGAGPQDSPSQ
TVFVTTWGSLFILPQLP*SPY*VGQPPSVTGRQE
G*VPYSLFTVATAPLPSAWDLSTYCGDGDAVTY
CPGEAQEPCGRHLRWAGAAPLTLLCFRHSAISP
SSDGAPITRSSPCPLPTPLLLLPAPQGSGSIFPL
FTNYLWTVVLFFVQCSILRHPSLLLLPAPNVHP
HCLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPV
PGKVGPCP*DAAAE*ARGPKSTIKGVGATSSPCS
VGEG*P*FVPAWGSLSGFLFAVT*IKKISFSGL
E
Frame 1
LL*LGLRAGAISGAGAAPRRREFAAAPHPSHHS
STCSLEKGR*VSRARSPASPWPPSRKRTALRSG
AGAGRASSLR*VPGQRW*GVRRSPAKCQHLRDLG
ADQREAKTRVLPRPGKPPQLQEGNQGADPKNWR
RRKRRASRRSPRRRSSDPCVPPAPHWRSSFLLG
LDSFAPLPPPPPLPQAHHHHRLWPPPPSSTCALT
TTLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHP
RIHTCPPGQG*HPT*PHPAPAASPLPWWWGHCS
LGFWFGGALSAPSLFPLASHSGAWEGSPGLKRGP
SPISSWHALLHCPGSSRCGQWRGVLAPRIPPAK
LSLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGR
RGESPTPSSLWPQPPCPPPGI*VHIVVMEMQSLI
VQVRPKSPVAAT*GGLGLLP*PYFASATQPFPP
PQMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFL
CSQTTSGQLCCFLFNVPFFDIRHCCCYQRQMFIL
IASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQS
QGRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPV
LLGRGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW
Frame 2
SSNWDSEPGLFLALARLQEGVSSRPLRIPAITL
PPAP*RREDE*VELEVQPALGLQAGKGRH*EAG
PGQAAQAASGESRDSAGRESEGAQRSANT*ETS
GPTKGKQKQGCCQDPENHHNSRKETKGQTQKTGE
GGRGGHLAGVLGGGAVTHACRLLLTGGAASFWD
WTALLRSHRPHPFPRPTITTASGRHPHLPPVPS
PPHYTAHQPLQGSHGLSGEQFSPGLSSQLPPPTH
AYTHALLDKANIPLSRTLHLLRPHSLGGGDIAL
WAFGLGAPSLLLHCSLWLPIVGPGRVPLALKGA
QAPSHPGTPYSTALAAAGVANGGGCWPPGFPQPN
CLCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAG
GVSPLLPLHCGHSPLALRLGSEYILW*WRCSHL
LSR*GPRALWPPPEVGWGCSPNPTLLPPLSHFPL
LRWGTNNKELTLPAPNPPPAPPCPPRFWFHFSS
VHKLPLDSCVVFCSMFHSSTSVIAAATSAKCSS
SLPPVLPTIPSPKILFVGKRGWGMAGWVTDYPSP
REGGALPLGCCSRVSKGAQIDHKGCRGHLLPLF
CWGGVAMICPSLGLPLWFPICSYLNKKNILFWT
G
Sequence HMGA1.vcAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCCATCCCAGCCATCACTCTTCCACCTGCTCCTT
AGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGC
GCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGG
AAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAG
GGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCT
CCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACA
GCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATG
CCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTT
GGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCAT
CCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACC
ACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCT
ACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGC
CCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCA
CCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACC
TCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTT
CTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGG
CCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGT
AGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
WRGRARPPCPWVALFKLP*AGAAIPAITLPPAP
*RREDE*VELEVQPALGLQAGKGRH*EAGPGQAA
QAASGESRDSAGRESEGAQRSANT*ETSGPTKG
KQKQGCCQDPENHHNSRKETKGQTQKTGEGGRG
GHLAGVLGGGAVTHACRLLLTGGAASFWDWTALL
RSHRPHPFPRPTITTASGRHPHLPPVPSPPHYT
AHQPLQGSHGLSGEQFSPGLSSQLPPPTHAYTH
ALLDKANIPLSRTLHLLRPHSLGGGDIALWAFGL
GAPSLLLHCSLWLPIVGPGRVPLALKGAQAPSH
PGTPYSTALAAAGVANGGGCWPPGFPQPNCLCH
HVGLTFHPSPTSLVPVLGWTAPFGYRKAGGVSPL
LPLHCGHSPLALRLGSEYILW*WRCSHLLSR*G
PRALWPPPEVGWGCSPNPTLLPPLSHFPLLRWG
TNNKELTLPAPNPPPAPPCPPRFWFHFSSVHKLP
LDSCVVFCSMFHSSTSVIAAATSAKCSSSLPPV
LPTIPSPKILFVGKRGWGMAGWVTDYPSPREGG
ALPLGCCSRVSKGAQIDHKGCRGHLLPLFCWGGV
AMICPSLGLPLWFPICSYLNKKNILFWTG
Frame 1
GAGGPVPPAPGSLFLSSPEPVLPSQPSLFHLLL
REGKMSESSSKSSQPLASKQEKDGTEKRGRGRP
RKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKG
SKNKGAAKTRKTTTTPGRKPRGRPKKLEKEEEE
GISQESSEEEQ*PMRAACSSLEEQLPSGTGQLC
SAPTAPTPSPGPPSPPPLAATPIFHLCPHHHTTQ
HTSRCRAPMG*VGSSFPLASVPSSPRPPTHTHM
PSWTRLTSHLAAPCTCCVPTPLVVGTLLSGLLV
WGRPLCSFTVPSGFP*WGLGGFPWP*KGPKPHLI
LARPTPLPWQQQVWPMEGGAGPQDSPSQTVFVT
TWGSLFILPQLP*SPY*VGQPPSVTGRQEG*VP
YSLFTVATAPLPSAWDLSTYCGDGDAVTYCPGEA
QEPCGRHLRWAGAAPLTLLCFRHSAISPSSDGA
PITRSSPCPLPTPLLLLPAPQGSGSIFPLFTNY
LWTVVLFFVQCSILRHPSLLLLPAPNVHPHCLLF
CPRSPPPRYSLWGRGAGAWQAG*PTTPVPGKVG
PCP*DAAAE*ARGPKSTIKGVGATSSPCSVGEG
*P*FVPAWGSLSGFLFAVT*IKKISFSGLE
Frame 2
ARAGPSPLPLGRSF*APLSRCCHPSHHSSTCSL
EKGR*VSRARSPASPWPPSRKRTALRSGAGAGR
ASSLR*VPGQRW*GVRRSPAKCQHLRDLGADQR
EAKTRVLPRPGKPPQLQEGNQGADPKNWRRRKRR
ASRRSPRRRSSDPCVPPAPHWRSSFLLGLDSFA
PLPPPPPLPQAHHHHRLWPPPPSSTCALTTTLH
STPAAAGLPWAEWGAVFPWPQFPAPPAHPRIHTC
PPGQG*HPT*PHPAPAASPLPWWWGHCSLGFWF
GGALSAPSLFPLASHSGAWEGSPGLKRGPSPIS
SWHALLHCPGSSRCGQWRGVLAPRIPPAKLSLSP
RGAHFSSFPNFPSPRTRLDSPLRLQEGRRGESP
TPSSLWPQPPCPPPGI*VHIVVMEMQSLIVQVR
PKSPVAAT*GGLGLLP*PYFASATQPFPPPQMGH
Q*QGAHPARSQPPSCSSLPPKVLVPFFLCSQTT
SGQLCCFLFNVPFFDIRHCCCYQRQMFILIASC
SAHDPLPQDTLCGEEGLGHGRLGDRLPQSQGRWG
PAPRMLQQSEQGGPNRP*RV*GPPPPPVLLGRG
SHDLSQPGAPSLVSYLQLLE*KKYPFLDW
Sequence HMGA1.tAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG
CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA
GGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCA
CCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACC
CATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCA
TCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGG
GGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACC
CTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGC
TTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGG
TGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTA
GTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCC
GCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGG
CTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCC
CCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATT
CTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGG
GAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGG
GCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGT
TTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS
QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV
GSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKT
TTTPGRKPRGRPKKLEKEEEEGISQESSEEEQ*P
MRAACSSLEEQLPSGTGQLCSAPTAPTPSPGPP
SPPPLAATPIFHLCPHHHTTQHTSRCRAPMG*V
GSSFPLASVPSSPRPPTHTHMPSWTRLTSHLAAP
CTCCVPTPLVVGTLLSGLLVWGRPLCSFTVPSG
FP*WGLGGFPWP*KGPKPHLILARPTPLPWQQQ
VWPMEGGAGPQDSPSQTVFVTTWGSLFILPQLP*
SPY*VGQPPSVTGRQEG*VPYSLFTVATAPLPS
AWDLSTYCGDGDAVTYCPGEAQEPCGRHLRWAG
AAPLTLLCFRHSAISPSSDGAPITRSSPCPLPTP
LLLLPAPQGSGSIFPLFTNYLWTVVLFFVQCSI
LRHPSLLLLPAPNVHPHCLLFCPRSPPPRYSLW
GRGAGAWQAG*PTTPVPGKVGPCP*DAAAE*ARG
PKSTIKGVGATSSPCSVGEG*P*FVPAWGSLSG
FLFAVT*IKKISFSGLE
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP
ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW*
GVRRSPAKCQHLRDLGADQREAKTRVLPRPGKP
PQLQEGNQGADPKNWRRRKRRASRRSPRRRSSD
PCVPPAPHWRSSFLLGLDSFAPLPPPPPLPQAHH
HHRLWPPPPSSTCALTTTLHSTPAAAGLPWAEW
GAVFPWPQFPAPPAHPRIHTCPPGQG*HPT*PH
PAPAASPLPWWWGHCSLGFWFGGALSAPSLFPLA
SHSGAWEGSPGLKRGPSPISSWHALLHCPGSSR
CGQWRGVLAPRIPPAKLSLSPRGAHFSSFPNFP
SPRTRLDSPLRLQEGRRGESPTPSSLWPQPPCPP
PGI*VHIVVMEMQSLIVQVRPKSPVAAT*GGLG
LLP*PYFASATQPFPPPQMGHQ*QGAHPARSQP
PSCSSLPPKVLVPFFLCSQTTSGQLCCFLFNVPF
FDIRHCCCYQRQMFILIASCSAHDPLPQDTLCG
EEGLGHGRLGDRLPQSQGRWGPAPRMLQQSEQG
GPNRP*RV*GPPPPPVLLGRGSHDLSQPGAPSLV
SYLQLLE*KKYPFLDW
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ
PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG
RESEGAQRSANT*ETSGPTKGKQKQGCCQDPENH
HNSRKETKGQTQKTGEGGRGGHLAGVLGGGAVT
HACRLLLTGGAASFWDWTALLRSHRPHPFPRPT
ITTASGRHPHLPPVPSPPHYTAHQPLQGSHGLSG
EQFSPGLSSQLPPPTHAYTHALLDKANIPLSRT
LHLLRPHSLGGGDIALWAFGLGAPSLLLHCSLW
LPIVGPGRVPLALKGAQAPSHPGTPYSTALAAAG
VANGGGCWPPGFPQPNCLCHHVGLTFHPSPTSL
VPVLGWTAPFGYRKAGGVSPLLPLHCGHSPLAL
RLGSEYILW*WRCSHLLSR*GPRALWPPPEVGWG
CSPNPTLLPPLSHFPLLRWGTNNKELTLPAPNP
PPAPPCPPRFWFHFSSVHKLPLDSCVVFCSMFH
SSTSVIAAATSAKCSSSLPPVLPTIPSPKILFVG
KRGWGMAGWVTDYPSPREGGALPLGCCSRVSKG
AQIDHKGCRGHLLPLFCWGGVAMICPSLGLPLW
FPICSYLNKKNILFWTG
Sequence HMGA1.kAug05
GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGATCCGCATTTGCTACCAG
CGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATC
CCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGC
ACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACAC
CTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACC
CAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAG
CTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCC
ACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTC
CCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTG
GGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGC
CTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGA
TTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTT
ACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAG
ATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTC
AGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGT
TCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGC
CAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACC
GACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACC
TCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATAT
CCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
APLIGTPSRGYFWRWRGSKKA*VRGRSDPHLLP
AAAAAEPGRSSAPSTAAPGNPERAPQAGGRARAS
QPSLFHLLLREGKMSESSSKSSQPLASKQEKDG
TEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPT
PKRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRP
KKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQ
LPSGTGQLCSAPTAPTPSPGPPSPPPLAATPIF
HLCPHHHTTQHTSRCRAPMG*VGSSFPLASVPSS
PRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVV
GTLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPW
P*KGPKPHLILARPTPLPWQQQVWPMEGGAGPQD
SPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSV
TGRQEG*VPYSLFTVATAPLPSAWDLSTYCGDG
DAVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRHS
AISPSSDGAPITRSSPCPLPTPLLLLPAPQGSG
SIFPLFTNYLWTVVLFFVQCSILRHPSLLLLPA
PNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*P
TTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGAT
SSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKI
SFSGLE
Frame 1
LL*LGLRAGAISGAGAAPRRREFAAAPIRICYQ
RRPRRSQAGPQRPAPPLPATRSAHRRPAAELAH
PSHHSSTCSLEKGR*VSRARSPASPWPPSRKRTA
LRSGAGAGRASSLR*VPGQRW*GVRRSPAKCQH
LRDLGADQREAKTRVLPRPGKPPQLQEGNQGAD
PKNWRRRKRRASRRSPRRRSSDPCVPPAPHWRSS
FLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSS
TCALTTTLHSTPAAAGLPWAEWGAVFPWPQFPA
PPAHPRIHTCPPGQG*HPT*PHPAPAASPLPWWW
GHCSLGFWFGGALSAPSLFPLASHSGAWEGSPG
LKRGPSPISSWHALLHCPGSSRCGQWRGVLAPR
IPPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLRL
QEGRRGESPTPSSLWPQPPCPPPGI*VHIVVME
MQSLIVQVRPKSPVAAT*GGLGLLP*PYFASAT
QPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVLV
PFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQR
QMFILIASCSAHDPLPQDTLCGEEGLGHGRLGD
RLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GPP
PPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKY
PFLDW
Frame 2
SSNWDSEPGLFLALARLQEGVSSRPLRSAFATS
GGRGGARPVLSAQHRRSRQPGARTAGRRPSSRI
PAITLPPAP*RREDE*VELEVQPALGLQAGKGR
H*EAGPGQAAQAASGESRDSAGRESEGAQRSANT
*ETSGPTKGKQKQGCCQDPENHHNSRKETKGQT
QKTGEGGRGGHLAGVLGGGAVTHACRLLLTGGA
ASFWDWTALLRSHRPHPFPRPTITTASGRHPHLP
PVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQL
PPPTHAYTHALLDKANIPLSRTLHLLRPHSLGG
GDIALWAFGLGAPSLLLHCSLWLPIVGPGRVPLA
LKGAQAPSHPGTPYSTALAAAGVANGGGCWPPG
FPQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFG
YRKAGGVSPLLPLHCGHSPLALRLGSEYILW*WR
CSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPL
SHFPLLRWGTNNKELTLPAPNPPPAPPCPPRFW
FHFSSVHKLPLDSCVVFCSMFHSSTSVIAAATSA
KCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVT
DYPSPREGGALPLGCCSRVSKGAQIDHKGCRGH
LLPLFCWGGVAMICPSLGLPLWFPICSYLNKKNI
LFWTG
Sequence HMGA1.fAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG
GGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAA
GACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAG
GAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTT
CCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCC
CATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCC
CACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCA
CTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCC
CTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTT
CCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAG
CCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCT
GAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGC
CCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTG
TTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAG
ATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCA
GAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGG
GCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP
GTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAK
TRKTTTTPGRKPRGRPKKLEKEEEEGISQESSE
EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTP
SPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAP
MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS
HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSF
TVPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP
WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL
PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVAT
APLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL
RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC
PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFF
VQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR
YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA
E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAW
GSLSGFLFAVT*IKKISFSGLE
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP
GQRW*GVRRSPAKCQHLRDLGADQREAKTRVLP
RPGKPPQLQEGNQGADPKNWRRRKRRASRRSPRR
RSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL
PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGL
PWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP
T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS
LFPLASHSGAWEGSPGLKRGPSPISSWHALLHC
PGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF
PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ
PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT
*GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA
RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL
FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQ
DTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ
SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG
APSLVSYLQLLE*KKYPFLDW
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRESEGAQRSANT*ETSGPTKGKQKQGCCQ
DPENHHNSRKETKGQTQKTGEGGRGGHLAGVLG
GGAVTHACRLLLTGGAASFWDWTALLRSHRPHPF
PRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS
HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANI
PLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH
CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA
LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHP
SPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS
PLALRLGSEYILW*WRCSHLLSR*GPRALWPPP
EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTL
PAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC
SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK
ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCS
RVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG
LPLWFPICSYLNKKNILFWTG
Sequence HMGA1.eAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCG
GCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAG
CTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGG
ACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGA
CCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGA
GGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCC
CCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCA
TGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCA
CTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACT
GTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCT
GGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCC
CCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCC
CCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGA
GGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCC
GCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTT
CAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGAT
ACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGA
GTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGC
TCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRSAFATSGGRGGARPVLSAQHRRSR
QPGARTAGRRPSSRIPAITLPPAP*RREDE*VE
LEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRESEGAQRSANT*ETSGPTKGKQKQGCCQD
PENHHNSRKETKGQTQKTGEGGRGGHLAGVLGG
GAVTHACRLLLTGGAASFWDWTALLRSHRPHPF
PRPTITTASGRHPHLPPVPSPPHYTAHQPLQGSH
GLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP
LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH
CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTAL
AAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS
PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS
PLALRLGSEYILW*WRCSHLLSR*GPRALWPPPE
VGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP
APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC
SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPKI
LFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR
VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG
LPLWFPICSYLNKKNILFWTG
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEDPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSESS
SKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPG
TALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAK
TRKTTTTPGRKPRGRPKKLEKEEEEGISQESSEE
EQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS
PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAP
MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSH
LAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT
VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP
WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFILP
QLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA
PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL
RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPCP
LPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV
QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR
YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE
*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG
SLSGFLFAVT*IKKISFSGLE
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKIRICYQRRPRRSQAGPQRPAPPLP
ATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VPG
QRW*GVRRSPAKCQHLRDLGADQREAKTRVLPR
PGKPPQLQEGNQGADPKNWRRRKRRASRRSPRR
RSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLP
QAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP
WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP
T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPSL
FPLASHSGAWEGSPGLKRGPSPISSWHALLHCP
GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF
PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQP
PCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*
GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA
RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLF
NVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD
TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ
SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGA
PSLVSYLQLLE*KKYPFLDW
Sequence HMGA1.cAug05
GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT
ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG
TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC
AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC
GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT
CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGA
GCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGA
AGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCT
GCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCT
GGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCC
CTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCG
TCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGG
CCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAG
GGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGG
TTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAG
TACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACC
CTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCC
CTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTC
ATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGG
GCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCA
TAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGT
TACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL
YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC
PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF
RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG
RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL
QAGKGRH*EAGPGQAAQAASGESRDSAGRESEG
AQRSANT*ETSGPTKGKQKQGCCQDPENHHNSR
KETKGQTQKTGEGGRGGHLAGVLGGGAVTHACRL
LLTGGAASFWDWTALLRSHRPHPFPRPTITTAS
GRHPHLPPVPSPPHYTAHQPLQGSHGLSGEQFS
PGLSSQLPPPTHAYTHALLDKANIPLSRTLHLLR
PHSLGGGDIALWAFGLGAPSLLLHCSLWLPIVG
PGRVPLALKGAQAPSHPGTPYSTALAAAGVANG
GGCWPPGFPQPNCLCHHVGLTFHPSPTSLVPVLG
WTAPFGYRKAGGVSPLLPLHCGHSPLALRLGSE
YILW*WRCSHLLSR*GPRALWPPPEVGWGCSPN
PTLLPPLSHFPLLRWGTNNKELTLPAPNPPPAPP
CPPRFWFHFSSVHKLPLDSCVVFCSMFHSSTSV
IAAATSAKCSSSLPPVLPTIPSPKILFVGKRGW
GMAGWVTDYPSPREGGALPLGCCSRVSKGAQIDH
KGCRGHLLPLFCWGGVAMICPSLGLPLWFPICS
YLNKKNILFWTG
Frame 1
LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY
IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF
VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS
DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA
GGRARASQPSLFHLLLREGKMSESSSKSSQPLA
SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKE
PSEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPG
RKPRGRPKKLEKEEEEGISQESSEEEQ*PMRAA
CSSLEEQLPSGTGQLCSAPTAPTPSPGPPSPPPL
AATPIFHLCPHHHTTQHTSRCRAPMG*VGSSFP
LASVPSSPRPPTHTHMPSWTRLTSHLAAPCTCC
VPTPLVVGTLLSGLLVWGRPLCSFTVPSGFP*WG
LGGFPWP*KGPKPHLILARPTPLPWQQQVWPME
GGAGPQDSPSQTVFVTTWGSLFILPQLP*SPY*
VGQPPSVTGRQEG*VPYSLFTVATAPLPSAWDLS
TYCGDGDAVTYCPGEAQEPCGRHLRWAGAAPLT
LLCFRHSAISPSSDGAPITRSSPCPLPTPLLLL
PAPQGSGSIFPLFTNYLWTVVLFFVQCSILRHPS
LLLLPAPNVHPHCLLFCPRSPPPRYSLWGRGAG
AWQAG*PTTPVPGKVGPCP*DAAAE*ARGPKST
IKGVGATSSPCSVGEG*P*FVPAWGSLSGFLFAV
T*IKKISFSGLE
Frame 2
SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI
SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL
SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF
QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP
AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP
PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRR
SPAKCQHLRDLGADQREAKTRVLPRPGKPPQLQE
GNQGADPKNWRRRKRRASRRSPRRRSSDPCVPP
APHWRSSFLLGLDSFAPLPPPPPLPQAHHHHRL
WPPPPSSTCALTTTLHSTPAAAGLPWAEWGAVFP
WPQFPAPPAHPRIHTCPPGQG*HPT*PHPAPAA
SPLPWWWGHCSLGFWFGGALSAPSLFPLASHSG
AWEGSPGLKRGPSPISSWHALLHCPGSSRCGQWR
GVLAPRIPPAKLSLSPRGAHFSSFPNFPSPRTR
LDSPLRLQEGRRGESPTPSSLWPQPPCPPPGI*
VHIVVMEMQSLIVQVRPKSPVAAT*GGLGLLP*P
YFASATQPFPPPQMGHQ*QGAHPARSQPPSCSS
LPPKVLVPFFLCSQTTSGQLCCFLFNVPFFDIR
HCCCYQRQMFILIASCSAHDPLPQDTLCGEEGLG
HGRLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP
*RV*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQ
LLE*KKYPFLDW
Sequence HMGA1.vkAug05
GTTGGAACTAGTCCCTTTGACTCCCCGTTTCTATTTTATTTTCTTCTGGGGCTTTATTTTGCGGGGAGGGGACGGAGGGAGGGGGCTTTCTTCCTCTCCC
CCGCGCCCGCGCGCGCCAGGTTTCCTGCCCCAAGGCGGGCTTGGGTGCCCCCTCTGGCAGGAAGTGGGGCCGGGTGCACCCGCGTGGCGGTGCATGCGGT
CGGGGTGCGCTGCTTTCCCGCGCCTGCAGGAGCGGCCTGCGCCCCTCCCCCCGCGCGCCCTGGCCCCGGGTCCCGCCGGCCGGCCTGCAACGGCCGGGCG
CAGACACTTGCAGAGTCACCCTCGGGCCGGGTCTGGAGCCTGATGCCTCGGGGGTTTAGCCCTAGCCGCTAGGCGGCATCCCAGCCATCACTCTTCCACC
TGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGG
CAGGCCGCGCAAGCAGCCTCCGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACC
CGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGG
AGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCC
AGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATG
GGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACT
TAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGT
TCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGG
CAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCC
AACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCC
CTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGG
TGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGC
TCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCA
ATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATAC
TCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGT
GAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTC
CCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
VGTSPFDSPFLFYFLLGLYFAGRGRREGAFFLS
PAPARARFPAPRRAWVPPLAGSGAGCTRVAVHAV
GVRCFPAPAGAACAPPPARPGPGSRRPACNGRA
QTLAESPSGRVWSLMPRGFSPSR*AASQPSLFH
LLLREGKMSESSSKSSQPLASKQEKDGTEKRGRG
RPRKQPPEPSEVPTPKRPRGRPKGSKNKGAAKT
RKTTTTPGRKPRGRPKKLEKEEEEGISQESSEE
EQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPSP
GPPSPPPLAATPIFHLCPHHHTTQHTSRCRAPM
G*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSH
LAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFTV
PSGFP*WGLGGFPWP*KGPKPHLILARPTPLPW
QQQVWPMEGGAGPQDSPSQTVFVTTWGSLFILP
QLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATAP
LPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLR
WAGAAPLTLLCFRHSAISPSSDGAPITRSSPCP
LPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFVQ
CSILRHPSLLLLPAPNVHPHCLLFCPRSPPPRY
SLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE
*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWGS
LSGFLFAVT*IKKISFSGLE
Frame 1
LELVPLTPRFYFIFFWGFILRGGDGGRGLSSSP
PRPRAPGFLPQGGLGCPLWQEVGPGAPAWRCMR
SGCAAFPRLQERPAPLPPRALAPGPAGRPATAGR
RHLQSHPRAGSGA*CLGGLALAARRHPSHHSST
CSLEKGR*VSRARSPASPWPPSRKRTALRSGAG
AGRASSLRSPAKCQHLRDLGADQREAKTRVLPRP
GKPPQLQEGNQGADPKNWRRRKRRASRRSPRRR
SSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLP
QAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLPW
AEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT
*PHPAPAASPLPWWWGHCSLGFWFGGALSAPSL
FPLASHSGAWEGSPGLKRGPSPISSWHALLHCPG
SSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFP
NFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQP
PCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*G
GLGLLP*PYFASATQPFPPPQMGHQ*QGAHPAR
SQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLF
NVPFFDIRHCCCYQRQMFILIASCSAHDPLPQDT
LCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQS
EQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGA
PSLVSYLQLLE*KKYPFLDW
Frame 2
WN*SL*LPVSILFSSGALFCGEGTEGGGFLPLP
RARARQVSCPKAGLGAPSGRKWGRVHPRGGACG
RGALLSRACRSGLRPSPRAPWPRVPPAGLQRPG
ADTCRVTLGPGLEPDASGV*P*PLGGIPAITLPP
AP*RREDE*VELEVQPALGLQAGKGRH*EAGPG
QAAQAASGAQRSANT*ETSGPTKGKQKQGCCQD
PENHHNSRKETKGQTQKTGEGGRGGHLAGVLGGG
AVTHACRLLLTGGAASFWDWTALLRSHRPHPFP
RPTITTASGRHPHLPPVPSPPHYTAHQPLQGSH
GLSGEQFSPGLSSQLPPPTHAYTHALLDKANIPL
SRTLHLLRPHSLGGGDIALWAFGLGAPSLLLHC
SLWLPIVGPGRVPLALKGAQAPSHPGTPYSTAL
AAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPSP
TSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSP
LALRLGSEYILW*WRCSHLLSR*GPRALWPPPE
VGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLPA
PNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCS
MFHSSTSVIAAATSAKCSSSLPPVLPTIPSPKI
LFVGKRGWGMAGWVTDYPSPREGGALPLGCCSRV
SKGAQIDHKGCRGHLLPLFCWGGVAMICPSLGL
PLWFPICSYLNKKNILFWTG
Sequence HMGA1.veAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG
CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGAGCCCAGCGAAGTGCCAACACCT
AAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCA
AAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCT
TCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCAC
CTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCC
CCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGG
GACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCT
TAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATT
CCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTAC
AGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGAT
GCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAG
CCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTC
CATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCA
AATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGA
CTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTC
CTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCC
TTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS
QPLASKQEKDGTEKRGRGRPRKQPPEPSEVPTP
KRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRP
KKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQL
PSGTGQLCSAPTAPTPSPGPPSPPPLAATPIFH
LCPHHHTTQHTSRCRAPMG*VGSSFPLASVPSS
PRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVVG
TLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPWP
*KGPKPHLILARPTPLPWQQQVWPMEGGAGPQD
SPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSVT
GRQEG*VPYSLFTVATAPLPSAWDLSTYCGDGD
AVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRHS
AISPSSDGAPITRSSPCPLPTPLLLLPAPQGSGS
IFPLFTNYLWTVVLFFVQCSILRHPSLLLLPAP
NVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*P
TTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGATS
SPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKIS
FSGLE
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP
ASPWPPSRKRTALRSGAGAGRASSLRSPAKCQHL
RDLGADQREAKTRVLPRPGKPPQLQEGNQGADP
KNWRRRKRRASRRSPRRRSSDPCVPPAPHWRSS
FLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSST
CALTTTLHSTPAAAGLPWAEWGAVFPWPQFPAP
PAHPRIHTCPPGQG*HPT*PHPAPAASPLPWWW
GHCSLGFWFGGALSAPSLFPLASHSGAWEGSPGL
KRGPSPISSWHALLHCPGSSRCGQWRGVLAPRI
PPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLRL
QEGRRGESPTPSSLWPQPPCPPPGI*VHIVVMEM
QSLIVQVRPKSPVAAT*GGLGLLP*PYFASATQ
PFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVLV
PFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQRQ
MFILIASCSAHDPLPQDTLCGEEGLGHGRLGDR
LPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GPP
PPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKYP
FLDW
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ
PALGLQAGKGRH*EAGPGQAAQAASGAQRSANT
*ETSGPTKGKQKQGCCQDPENHHNSRKETKGQTQ
KTGEGGRGGHLAGVLGGGAVTHACRLLLTGGAA
SFWDWTALLRSHRPHPFPRPTITTASGRHPHLP
PVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQLP
PPTHAYTHALLDKANIPLSRTLHLLRPHSLGGG
DIALWAFGLGAPSLLLHCSLWLPIVGPGRVPLA
LKGAQAPSHPGTPYSTALAAAGVANGGGCWPPGF
PQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFGY
RKAGGVSPLLPLHCGHSPLALRLGSEYILW*WR
CSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPLS
HFPLLRWGTNNKELTLPAPNPPPAPPCPPRFWF
HFSSVHKLPLDSCVVFCSMFHSSTSVIAAATSA
KCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVTD
YPSPREGGALPLGCCSRVSKGAQIDHKGCRGHL
LPLFCWGGVAMICPSLGLPLWFPICSYLNKKNI
LFWTG
Sequence HMGA1.lAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGAGCCCAGCG
AAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACC
AAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCA
CTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCA
CCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTC
AGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACT
CCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAG
GGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGC
TGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAG
CCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATT
GTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTG
CTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCC
CAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGC
TGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCA
GGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGT
GTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAA
TAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPEPS
EVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRKP
RGRPKKLEKEEEEGISQESSEEEQ*PMRAACSS
LEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLAA
TPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPLAS
VPSSPRPPTHTHMPSWTRLTSHLAAPCTCCVPT
PLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGLG
GFPWP*KGPKPHLILARPTPLPWQQQVWPMEGGA
GPQDSPSQTVFVTTWGSLFILPQLP*SPY*VGQ
PPSVTGRQEG*VPYSLFTVATAPLPSAWDLSTY
CGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTLLC
FRHSAISPSSDGAPITRSSPCPLPTPLLLLPAP
QGSGSIFPLFTNYLWTVVLFFVQCSILRHPSLL
LLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGAWQ
AG*PTTPVPGKVGPCP*DAAAE*ARGPKSTIKG
VGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT*
IKKISFSGLE
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLRSPA
KCQHLRDLGADQREAKTRVLPRPGKPPQLQEGN
QGADPKNWRRRKRRASRRSPRRRSSDPCVPPAPH
WRSSFLLGLDSFAPLPPPPPLPQAHHHHRLWPP
PPSSTCALTTTLHSTPAAAGLPWAEWGAVFPWP
QFPAPPAHPRIHTCPPGQG*HPT*PHPAPAASPL
PWWWGHCSLGFWFGGALSAPSLFPLASHSGAWE
GSPGLKRGPSPISSWHALLHCPGSSRCGQWRGV
LAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRLDS
PLRLQEGRRGESPTPSSLWPQPPCPPPGI*VHI
VVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PYF
ASATQPFPPPQMGHQ*QGAHPARSQPPSCSSLPP
KVLVPFFLCSQTTSGQLCCFLFNVPFFDIRHCC
CYQRQMFILIASCSAHDPLPQDTLCGEEGLGHG
RLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV
*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQLLE
*KKYPFLDW
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASGAQR
SANT*ETSGPTKGKQKQGCCQDPENHHNSRKET
KGQTQKTGEGGRGGHLAGVLGGGAVTHACRLLL
TGGAASFWDWTALLRSHRPHPFPRPTITTASGRH
PHLPPVPSPPHYTAHQPLQGSHGLSGEQFSPGL
SSQLPPPTHAYTHALLDKANIPLSRTLHLLRPH
SLGGGDIALWAFGLGAPSLLLHCSLWLPIVGPGR
VPLALKGAQAPSHPGTPYSTALAAAGVANGGGC
WPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGWT
APFGYRKAGGVSPLLPLHCGHSPLALRLGSEYIL
W*WRCSHLLSR*GPRALWPPPEVGWGCSPNPTL
LPPLSHFPLLRWGTNNKELTLPAPNPPPAPPCP
PRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVIAA
ATSAKCSSSLPPVLPTIPSPKILFVGKRGWGMA
GWVTDYPSPREGGALPLGCCSRVSKGAQIDHKG
CRGHLLPLFCWGGVAMICPSLGLPLWFPICSYLN
KKNILFWTG