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Sequence HMGA1.vnAug05
GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTATATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG
TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC
AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC
GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT
CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGGAGAA
GGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACT
GGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACC
ACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCAC
GCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCT
GGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCA
AGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAAC
TGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAG
GGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATT
GTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTC
CTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTG
TTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTC
ATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCC
AGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCT
GTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGG
AG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Single exon skipping
Frame 0
APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFLYL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC
PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF
RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG
RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL
QAGKGRH*EAGPGQAAQAASGESRDSAGRESGE
GGRGGHLAGVLGGGAVTHACRLLLTGGAASFWD
WTALLRSHRPHPFPRPTITTASGRHPHLPPVPSP
PHYTAHQPLQGSHGLSGEQFSPGLSSQLPPPTH
AYTHALLDKANIPLSRTLHLLRPHSLGGGDIAL
WAFGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQ
APSHPGTPYSTALAAAGVANGGGCWPPGFPQPN
CLCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAG
GVSPLLPLHCGHSPLALRLGSEYILW*WRCSHLL
SR*GPRALWPPPEVGWGCSPNPTLLPPLSHFPL
LRWGTNNKELTLPAPNPPPAPPCPPRFWFHFSS
VHKLPLDSCVVFCSMFHSSTSVIAAATSAKCSSS
LPPVLPTIPSPKILFVGKRGWGMAGWVTDYPSP
REGGALPLGCCSRVSKGAQIDHKGCRGHLLPLF
CWGGVAMICPSLGLPLWFPICSYLNKKNILFWTG
Frame 1
LL*LGLRAGAISGAGAAPRRREFAAAPVASFFYIYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF
VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS
DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA
GGRARASQPSLFHLLLREGKMSESSSKSSQPLA
SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQEK
EEEEGISQESSEEEQ*PMRAACSSLEEQLPSGT
GQLCSAPTAPTPSPGPPSPPPLAATPIFHLCPH
HHTTQHTSRCRAPMG*VGSSFPLASVPSSPRPPT
HTHMPSWTRLTSHLAAPCTCCVPTPLVVGTLLS
GLLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGP
KPHLILARPTPLPWQQQVWPMEGGAGPQDSPSQT
VFVTTWGSLFILPQLP*SPY*VGQPPSVTGRQE
G*VPYSLFTVATAPLPSAWDLSTYCGDGDAVTY
CPGEAQEPCGRHLRWAGAAPLTLLCFRHSAISPS
SDGAPITRSSPCPLPTPLLLLPAPQGSGSIFPL
FTNYLWTVVLFFVQCSILRHPSLLLLPAPNVHP
HCLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVP
GKVGPCP*DAAAE*ARGPKSTIKGVGATSSPCS
VGEG*P*FVPAWGSLSGFLFAVT*IKKISFSGL
E
Frame 2
SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFISII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL
SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF
QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP
AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP
PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRR
RRKRRASRRSPRRRSSDPCVPPAPHWRSSFLLGL
DSFAPLPPPPPLPQAHHHHRLWPPPPSSTCALT
TTLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHP
RIHTCPPGQG*HPT*PHPAPAASPLPWWWGHCSL
GFWFGGALSAPSLFPLASHSGAWEGSPGLKRGP
SPISSWHALLHCPGSSRCGQWRGVLAPRIPPAK
LSLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRR
GESPTPSSLWPQPPCPPPGI*VHIVVMEMQSLI
VQVRPKSPVAAT*GGLGLLP*PYFASATQPFPP
PQMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLC
SQTTSGQLCCFLFNVPFFDIRHCCCYQRQMFIL
IASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQS
QGRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVL
LGRGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW
Sequence HMGA1.vmAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG
CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA
GGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCA
TCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGC
TCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCAC
ACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCT
CCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGG
GCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTG
GCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGT
GGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTC
CCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAA
GAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAA
TAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTG
GACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGC
CCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCT
GCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCC
ATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Single exon skipping
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGAISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS
QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV
GSQKEPSEVPTPKRPRGRPKGSKNKGEKEEEEG
ISQESSEEEQ*PMRAACSSLEEQLPSGTGQLCSA
PTAPTPSPGPPSPPPLAATPIFHLCPHHHTTQH
TSRCRAPMG*VGSSFPLASVPSSPRPPTHTHMP
SWTRLTSHLAAPCTCCVPTPLVVGTLLSGLLVWG
RPLCSFTVPSGFP*WGLGGFPWP*KGPKPHLIL
ARPTPLPWQQQVWPMEGGAGPQDSPSQTVFVTT
WGSLFILPQLP*SPY*VGQPPSVTGRQEG*VPYS
LFTVATAPLPSAWDLSTYCGDGDAVTYCPGEAQ
EPCGRHLRWAGAAPLTLLCFRHSAISPSSDGAP
ITRSSPCPLPTPLLLLPAPQGSGSIFPLFTNYLW
TVVLFFVQCSILRHPSLLLLPAPNVHPHCLLFC
PRSPPPRYSLWGRGAGAWQAG*PTTPVPGKVGP
CP*DAAAE*ARGPKSTIKGVGATSSPCSVGEG*P
*FVPAWGSLSGFLFAVT*IKKISFSGLE
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGLFLALARLQEGHPSHHSSTCSLEKGR*VSRARSP
ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW*
GVRRSPAKCQHLRDLGADQREAKTRVRRRKRRA
SRRSPRRRSSDPCVPPAPHWRSSFLLGLDSFAP
LPPPPPLPQAHHHHRLWPPPPSSTCALTTTLHST
PAAAGLPWAEWGAVFPWPQFPAPPAHPRIHTCP
PGQG*HPT*PHPAPAASPLPWWWGHCSLGFWFG
GALSAPSLFPLASHSGAWEGSPGLKRGPSPISSW
HALLHCPGSSRCGQWRGVLAPRIPPAKLSLSPR
GAHFSSFPNFPSPRTRLDSPLRLQEGRRGESPT
PSSLWPQPPCPPPGI*VHIVVMEMQSLIVQVRPK
SPVAAT*GGLGLLP*PYFASATQPFPPPQMGHQ
*QGAHPARSQPPSCSSLPPKVLVPFFLCSQTTS
GQLCCFLFNVPFFDIRHCCCYQRQMFILIASCSA
HDPLPQDTLCGEEGLGHGRLGDRLPQSQGRWGP
APRMLQQSEQGGPNRP*RV*GPPPPPVLLGRGS
HDLSQPGAPSLVSYLQLLE*KKYPFLDW
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGYFWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ
PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG
RESEGAQRSANT*ETSGPTKGKQKQG*EGGRGGH
LAGVLGGGAVTHACRLLLTGGAASFWDWTALLR
SHRPHPFPRPTITTASGRHPHLPPVPSPPHYTA
HQPLQGSHGLSGEQFSPGLSSQLPPPTHAYTHAL
LDKANIPLSRTLHLLRPHSLGGGDIALWAFGLG
APSLLLHCSLWLPIVGPGRVPLALKGAQAPSHP
GTPYSTALAAAGVANGGGCWPPGFPQPNCLCHHV
GLTFHPSPTSLVPVLGWTAPFGYRKAGGVSPLL
PLHCGHSPLALRLGSEYILW*WRCSHLLSR*GP
RALWPPPEVGWGCSPNPTLLPPLSHFPLLRWGTN
NKELTLPAPNPPPAPPCPPRFWFHFSSVHKLPL
DSCVVFCSMFHSSTSVIAAATSAKCSSSLPPVL
PTIPSPKILFVGKRGWGMAGWVTDYPSPREGGAL
PLGCCSRVSKGAQIDHKGCRGHLLPLFCWGGVA
MICPSLGLPLWFPICSYLNKKNILFWTG
Sequence HMGA1.nAug05
GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTATATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG
TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC
AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC
GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT
CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGAAGGA
GCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCC
TCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCA
CCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAG
GGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTA
ACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCT
CCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCC
ACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTC
ATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGG
CCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCG
CCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCA
CCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGT
TTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCC
CCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCT
GCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAG
CCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Single exon skipping
Frame 0
APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFLYL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC
PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF
RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG
RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL
QAGKGRH*EAGPGQAAQAASGESRDSAGRESEG
AQRSANT*ETSGPTKGKQKQG*EGGRGGHLAGV
LGGGAVTHACRLLLTGGAASFWDWTALLRSHRPH
PFPRPTITTASGRHPHLPPVPSPPHYTAHQPLQ
GSHGLSGEQFSPGLSSQLPPPTHAYTHALLDKA
NIPLSRTLHLLRPHSLGGGDIALWAFGLGAPSLL
LHCSLWLPIVGPGRVPLALKGAQAPSHPGTPYS
TALAAAGVANGGGCWPPGFPQPNCLCHHVGLTF
HPSPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCG
HSPLALRLGSEYILW*WRCSHLLSR*GPRALWP
PPEVGWGCSPNPTLLPPLSHFPLLRWGTNNKEL
TLPAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVV
FCSMFHSSTSVIAAATSAKCSSSLPPVLPTIPS
PKILFVGKRGWGMAGWVTDYPSPREGGALPLGC
CSRVSKGAQIDHKGCRGHLLPLFCWGGVAMICPS
LGLPLWFPICSYLNKKNILFWTG
Frame 1
LL*LGLRAGAISGAGAAPRRREFAAAPVASFFYIYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF
VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS
DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA
GGRARASQPSLFHLLLREGKMSESSSKSSQPLA
SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKE
PSEVPTPKRPRGRPKGSKNKGEKEEEEGISQES
SEEEQ*PMRAACSSLEEQLPSGTGQLCSAPTAP
TPSPGPPSPPPLAATPIFHLCPHHHTTQHTSRCR
APMG*VGSSFPLASVPSSPRPPTHTHMPSWTRL
TSHLAAPCTCCVPTPLVVGTLLSGLLVWGRPLC
SFTVPSGFP*WGLGGFPWP*KGPKPHLILARPTP
LPWQQQVWPMEGGAGPQDSPSQTVFVTTWGSLF
ILPQLP*SPY*VGQPPSVTGRQEG*VPYSLFTV
ATAPLPSAWDLSTYCGDGDAVTYCPGEAQEPCGR
HLRWAGAAPLTLLCFRHSAISPSSDGAPITRSS
PCPLPTPLLLLPAPQGSGSIFPLFTNYLWTVVL
FFVQCSILRHPSLLLLPAPNVHPHCLLFCPRSPP
PRYSLWGRGAGAWQAG*PTTPVPGKVGPCP*DA
AAE*ARGPKSTIKGVGATSSPCSVGEG*P*FVP
AWGSLSGFLFAVT*IKKISFSGLE
Frame 2
SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFISII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL
SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF
QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP
AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP
PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRR
SPAKCQHLRDLGADQREAKTRVRRRKRRASRRSP
RRRSSDPCVPPAPHWRSSFLLGLDSFAPLPPPP
PLPQAHHHHRLWPPPPSSTCALTTTLHSTPAAA
GLPWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*
HPT*PHPAPAASPLPWWWGHCSLGFWFGGALSA
PSLFPLASHSGAWEGSPGLKRGPSPISSWHALL
HCPGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFS
SFPNFPSPRTRLDSPLRLQEGRRGESPTPSSLW
PQPPCPPPGI*VHIVVMEMQSLIVQVRPKSPVA
AT*GGLGLLP*PYFASATQPFPPPQMGHQ*QGAH
PARSQPPSCSSLPPKVLVPFFLCSQTTSGQLCC
FLFNVPFFDIRHCCCYQRQMFILIASCSAHDPL
PQDTLCGEEGLGHGRLGDRLPQSQGRWGPAPRML
QQSEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQ
PGAPSLVSYLQLLE*KKYPFLDW
Sequence HMGA1.mAug05
TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG
GGACAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGA
GGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGA
CAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACC
ACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCA
TACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGG
CTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGC
CCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGT
CTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGG
TGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTC
CAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTC
AGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTC
ACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATT
GCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGG
GAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTT
GGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
- HMGA1.au.0.f (forward primer)
- HMGA1.u.r.18 (reverse primer)
- Single exon skipping
Frame 0
WRGRARPPCPWVALFKLP*AGAALL*LGLRAGAISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP
GTALVGSQKEPSEVPTPKRPRGRPKGSKNKGEKE
EEEGISQESSEEEQ*PMRAACSSLEEQLPSGTG
QLCSAPTAPTPSPGPPSPPPLAATPIFHLCPHH
HTTQHTSRCRAPMG*VGSSFPLASVPSSPRPPTH
THMPSWTRLTSHLAAPCTCCVPTPLVVGTLLSG
LLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGPK
PHLILARPTPLPWQQQVWPMEGGAGPQDSPSQTV
FVTTWGSLFILPQLP*SPY*VGQPPSVTGRQEG
*VPYSLFTVATAPLPSAWDLSTYCGDGDAVTYC
PGEAQEPCGRHLRWAGAAPLTLLCFRHSAISPSS
DGAPITRSSPCPLPTPLLLLPAPQGSGSIFPLF
TNYLWTVVLFFVQCSILRHPSLLLLPAPNVHPH
CLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVPG
KVGPCP*DAAAE*ARGPKSTIKGVGATSSPCSV
GEG*P*FVPAWGSLSGFLFAVT*IKKISFSGLE
Frame 1
GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGLFLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP
GQRW*GVRRSPAKCQHLRDLGADQREAKTRVRR
RKRRASRRSPRRRSSDPCVPPAPHWRSSFLLGLD
SFAPLPPPPPLPQAHHHHRLWPPPPSSTCALTT
TLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHPR
IHTCPPGQG*HPT*PHPAPAASPLPWWWGHCSLG
FWFGGALSAPSLFPLASHSGAWEGSPGLKRGPS
PISSWHALLHCPGSSRCGQWRGVLAPRIPPAKL
SLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRRG
ESPTPSSLWPQPPCPPPGI*VHIVVMEMQSLIV
QVRPKSPVAAT*GGLGLLP*PYFASATQPFPPP
QMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLCS
QTTSGQLCCFLFNVPFFDIRHCCCYQRQMFILI
ASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQSQ
GRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVLL
GRGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW
Frame 2
ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGYFWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRESEGAQRSANT*ETSGPTKGKQKQG*EG
GRGGHLAGVLGGGAVTHACRLLLTGGAASFWDW
TALLRSHRPHPFPRPTITTASGRHPHLPPVPSPP
HYTAHQPLQGSHGLSGEQFSPGLSSQLPPPTHA
YTHALLDKANIPLSRTLHLLRPHSLGGGDIALW
AFGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQA
PSHPGTPYSTALAAAGVANGGGCWPPGFPQPNC
LCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAGG
VSPLLPLHCGHSPLALRLGSEYILW*WRCSHLLS
R*GPRALWPPPEVGWGCSPNPTLLPPLSHFPLL
RWGTNNKELTLPAPNPPPAPPCPPRFWFHFSSV
HKLPLDSCVVFCSMFHSSTSVIAAATSAKCSSSL
PPVLPTIPSPKILFVGKRGWGMAGWVTDYPSPR
EGGALPLGCCSRVSKGAQIDHKGCRGHLLPLFC
WGGVAMICPSLGLPLWFPICSYLNKKNILFWTG
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