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Sequences

Sequence HMGA1.qAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA
GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGA
GGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCT
TCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTC
CCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATC
CCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTC
ACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGC
CCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCT
TCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACA
GCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACC
TGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTG
CCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTT
GTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAA
GATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGC
AGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGG
GGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Alternative donor and acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP
SEVPTPKRPRGRPKGSKNKGEKEEEEGISQESSE
EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTP
SPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRA
PMG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS
HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSF
TVPSGFP*WGLGGFPWP*KGPKPHLILARPTPL
PWQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL
PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVAT
APLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRH
LRWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC
PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFF
VQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPP
RYSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA
E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAW
GSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP
AKCQHLRDLGADQREAKTRVRRRKRRASRRSPR
RRSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL
PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGL
PWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*H
PT*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS
LFPLASHSGAWEGSPGLKRGPSPISSWHALLHC
PGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSS
FPNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ
PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT
*GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHP
ARSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL
FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQ
DTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQ
QSEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG
APSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ
RSANT*ETSGPTKGKQKQG*EGGRGGHLAGVLG
GGAVTHACRLLLTGGAASFWDWTALLRSHRPHP
FPRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS
HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANI
PLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLL
HCSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA
LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHP
SPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGH
SPLALRLGSEYILW*WRCSHLLSR*GPRALWPPP
EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTL
PAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVF
CSMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK
ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCS
RVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSL
GLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.vlAug05

GGGTCCCCAGCAGCAAGAGGTGGGGGGAGGCACCAGATGGTATGAGAGCTTCCAGGGAGACCCGCCAAGATCTCCAGGCAGGCCGGGCCAGGCTCTCTGG
GATTCCGGACTGGCTTCGCCCAACTGGAACCCCCTCGATGAGGGGGCCCCAGGCAGACCTTATATGAGCATCCCAGCCATCACTCTTCCACCTGCTCCTT
AGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGC
GCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAA
AACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAG
TGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCC
CACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTG
AGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCC
GCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCT
CTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCA
GCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTT
CCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGC
CCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGC
TGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCA
ACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTT
CCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTT
GTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCA
AGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCT
CTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Alternative donor and acceptor

Frame 0

GSPAARGGGRHQMV*ELPGRPAKISRQAGPGSL
GFRTGFAQLEPPR*GGPRQTLYEHPSHHSSTCSL
EKGR*VSRARSPASPWPPSRKRTALRSGAGAGR
ASSLRRSPAKCQHLRDLGADQREAKTRVLPRPG
KPPQLQEGNQGADPKNWRRRKRRASRRSPRRRSS
DPCVPPAPHWRSSFLLGLDSFAPLPPPPPLPQA
HHHHRLWPPPPSSTCALTTTLHSTPAAAGLPWA
EWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT*P
HPAPAASPLPWWWGHCSLGFWFGGALSAPSLFP
LASHSGAWEGSPGLKRGPSPISSWHALLHCPGS
SRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFPNF
PSPRTRLDSPLRLQEGRRGESPTPSSLWPQPPC
PPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*GG
LGLLP*PYFASATQPFPPPQMGHQ*QGAHPARSQ
PPSCSSLPPKVLVPFFLCSQTTSGQLCCFLFNV
PFFDIRHCCCYQRQMFILIASCSAHDPLPQDTL
CGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQSEQ
GGPNRP*RV*GPPPPPVLLGRGSHDLSQPGAPS
LVSYLQLLE*KKYPFLDW

Frame 1

GPQQQEVGGGTRWYESFQGDPPRSPGRPGQALW
DSGLASPNWNPLDEGAPGRPYMSIPAITLPPAP
*RREDE*VELEVQPALGLQAGKGRH*EAGPGQAA
QAASEGAQRSANT*ETSGPTKGKQKQGCCQDPE
NHHNSRKETKGQTQKTGEGGRGGHLAGVLGGGA
VTHACRLLLTGGAASFWDWTALLRSHRPHPFPRP
TITTASGRHPHLPPVPSPPHYTAHQPLQGSHGL
SGEQFSPGLSSQLPPPTHAYTHALLDKANIPLS
RTLHLLRPHSLGGGDIALWAFGLGAPSLLLHCSL
WLPIVGPGRVPLALKGAQAPSHPGTPYSTALAA
AGVANGGGCWPPGFPQPNCLCHHVGLTFHPSPT
SLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSPLA
LRLGSEYILW*WRCSHLLSR*GPRALWPPPEVG
WGCSPNPTLLPPLSHFPLLRWGTNNKELTLPAP
NPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCSMF
HSSTSVIAAATSAKCSSSLPPVLPTIPSPKILF
VGKRGWGMAGWVTDYPSPREGGALPLGCCSRVS
KGAQIDHKGCRGHLLPLFCWGGVAMICPSLGLPL
WFPICSYLNKKNILFWTG

Frame 2

VPSSKRWGEAPDGMRASRETRQDLQAGRARLSG
IPDWLRPTGTPSMRGPQADLI*ASQPSLFHLLL
REGKMSESSSKSSQPLASKQEKDGTEKRGRGRP
RKQPPKEPSEVPTPKRPRGRPKGSKNKGAAKTRK
TTTTPGRKPRGRPKKLEKEEEEGISQESSEEEQ
*PMRAACSSLEEQLPSGTGQLCSAPTAPTPSPG
PPSPPPLAATPIFHLCPHHHTTQHTSRCRAPMG*
VGSSFPLASVPSSPRPPTHTHMPSWTRLTSHLA
APCTCCVPTPLVVGTLLSGLLVWGRPLCSFTVP
SGFP*WGLGGFPWP*KGPKPHLILARPTPLPWQQ
QVWPMEGGAGPQDSPSQTVFVTTWGSLFILPQL
P*SPY*VGQPPSVTGRQEG*VPYSLFTVATAPL
PSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLRWA
GAAPLTLLCFRHSAISPSSDGAPITRSSPCPLP
TPLLLLPAPQGSGSIFPLFTNYLWTVVLFFVQC
SILRHPSLLLLPAPNVHPHCLLFCPRSPPPRYSL
WGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE*A
RGPKSTIKGVGATSSPCSVGEG*P*FVPAWGSL
SGFLFAVT*IKKISFSGLE

Sequence HMGA1.vjAug05

GTTGGAACTAGTCCCTTTGACTCCCCGTTTCTATTTTATTTTCTTCTGGGGCTTTATTTTGCGGGGAGGGGACGGAGGGAGGGGGCTTTCTTCCTCTCCC
CCGCGCCCGCGCGCGCCAGGTTTCCTGCCCCAAGGCGGGCTTGGGTGCCCCCTCTGGCAGGAAGTGGGGCCGGGTGCACCCGCGTGGCGGTGCATGCGGT
CGGGGTGCGCTGCTTTCCCGCGCCTGCAGGAGCGGCCTGCGCCCCTCCCCCCGCGCGCCCTGGCCCCGGGTCCCGCCGGCCGGCCTGCAACGGCCGGGCG
CAGACACTTGCAGAGTCACCCTCGGGCCGGGTCTGGAGCCTGATGCCTCGGGGGTTTAGCCCTAGCCGCTAGGCGGCATCCCAGCCATCACTCTTCCACC
TGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGG
CAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAG
ACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGG
AGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTC
CCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCC
ATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCC
ACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCAC
TGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCC
TGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTC
CCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGC
CCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTG
AGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCC
CGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGT
TCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGA
TACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAG
AGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGG
CTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Alternative donor and acceptor

Frame 0

VGTSPFDSPFLFYFLLGLYFAGRGRREGAFFLS
PAPARARFPAPRRAWVPPLAGSGAGCTRVAVHAV
GVRCFPAPAGAACAPPPARPGPGSRRPACNGRA
QTLAESPSGRVWSLMPRGFSPSR*AASQPSLFH
LLLREGKMSESSSKSSQPLASKQEKDGTEKRGRG
RPRKQPPKEPSEVPTPKRPRGRPKGSKNKGAAK
TRKTTTTPGRKPRGRPKKLEKEEEEGISQESSE
EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS
PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAP
MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS
HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT
VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP
WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL
PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA
PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL
RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC
PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV
QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR
YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA
E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG
SLSGFLFAVT*IKKISFSGLE

Frame 1

LELVPLTPRFYFIFFWGFILRGGDGGRGLSSSP
PRPRAPGFLPQGGLGCPLWQEVGPGAPAWRCMR
SGCAAFPRLQERPAPLPPRALAPGPAGRPATAGR
RHLQSHPRAGSGA*CLGGLALAARRHPSHHSST
CSLEKGR*VSRARSPASPWPPSRKRTALRSGAG
AGRASSLRRSPAKCQHLRDLGADQREAKTRVLPR
PGKPPQLQEGNQGADPKNWRRRKRRASRRSPRR
RSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL
PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP
WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP
T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS
LFPLASHSGAWEGSPGLKRGPSPISSWHALLHCP
GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF
PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ
PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*
GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA
RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL
FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD
TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ
SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG
APSLVSYLQLLE*KKYPFLDW

Frame 2

WN*SL*LPVSILFSSGALFCGEGTEGGGFLPLP
RARARQVSCPKAGLGAPSGRKWGRVHPRGGACG
RGALLSRACRSGLRPSPRAPWPRVPPAGLQRPG
ADTCRVTLGPGLEPDASGV*P*PLGGIPAITLPP
AP*RREDE*VELEVQPALGLQAGKGRH*EAGPG
QAAQAASEGAQRSANT*ETSGPTKGKQKQGCCQ
DPENHHNSRKETKGQTQKTGEGGRGGHLAGVLGG
GAVTHACRLLLTGGAASFWDWTALLRSHRPHPF
PRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS
HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP
LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH
CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA
LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS
PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS
PLALRLGSEYILW*WRCSHLLSR*GPRALWPPP
EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP
APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC
SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK
ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR
VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG
LPLWFPICSYLNKKNILFWTG

Sequence HMGA1.vgAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCC
AGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACC
TAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCC
AAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGC
TTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCA
CCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCC
CCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGG
GGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCC
TTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGAT
TCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTA
CAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGA
TGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCA
GCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTT
CCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCC
AAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCG
ACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCT
CCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATC
CTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Alternative donor and acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRSIPAITLPPAP*RREDE*VELEVQP
ALGLQAGKGRH*EAGPGQAAQAASEGAQRSANT
*ETSGPTKGKQKQGCCQDPENHHNSRKETKGQT
QKTGEGGRGGHLAGVLGGGAVTHACRLLLTGGAA
SFWDWTALLRSHRPHPFPRPTITTASGRHPHLP
PVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQL
PPPTHAYTHALLDKANIPLSRTLHLLRPHSLGGG
DIALWAFGLGAPSLLLHCSLWLPIVGPGRVPLA
LKGAQAPSHPGTPYSTALAAAGVANGGGCWPPG
FPQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFGY
RKAGGVSPLLPLHCGHSPLALRLGSEYILW*WR
CSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPL
SHFPLLRWGTNNKELTLPAPNPPPAPPCPPRFWF
HFSSVHKLPLDSCVVFCSMFHSSTSVIAAATSA
KCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVT
DYPSPREGGALPLGCCSRVSKGAQIDHKGCRGHL
LPLFCWGGVAMICPSLGLPLWFPICSYLNKKNI
LFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEASQPSLFHLLLREGKMSESSSKSS
QPLASKQEKDGTEKRGRGRPRKQPPKEPSEVPTP
KRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRP
KKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQ
LPSGTGQLCSAPTAPTPSPGPPSPPPLAATPIFH
LCPHHHTTQHTSRCRAPMG*VGSSFPLASVPSS
PRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVV
GTLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPWP
*KGPKPHLILARPTPLPWQQQVWPMEGGAGPQD
SPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSV
TGRQEG*VPYSLFTVATAPLPSAWDLSTYCGDGD
AVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRHS
AISPSSDGAPITRSSPCPLPTPLLLLPAPQGSG
SIFPLFTNYLWTVVLFFVQCSILRHPSLLLLPAP
NVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*P
TTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGAT
SSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKIS
FSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKHPSHHSSTCSLEKGR*VSRARSPA
SPWPPSRKRTALRSGAGAGRASSLRRSPAKCQH
LRDLGADQREAKTRVLPRPGKPPQLQEGNQGADP
KNWRRRKRRASRRSPRRRSSDPCVPPAPHWRSS
FLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSS
TCALTTTLHSTPAAAGLPWAEWGAVFPWPQFPAP
PAHPRIHTCPPGQG*HPT*PHPAPAASPLPWWW
GHCSLGFWFGGALSAPSLFPLASHSGAWEGSPG
LKRGPSPISSWHALLHCPGSSRCGQWRGVLAPRI
PPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLRL
QEGRRGESPTPSSLWPQPPCPPPGI*VHIVVME
MQSLIVQVRPKSPVAAT*GGLGLLP*PYFASATQ
PFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVLV
PFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQR
QMFILIASCSAHDPLPQDTLCGEEGLGHGRLGDR
LPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GPP
PPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKY
PFLDW

Sequence HMGA1.vdAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG
CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACA
CCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGAC
CCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCA
GCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTC
CACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCT
CCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGT
GGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGG
CCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGG
ATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGT
TACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGA
GATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACT
CAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGG
TTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCG
CCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGAC
CGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCAC
CTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATA
TCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Alternative donor and acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS
QPLASKQEKDGTEKRGRGRPRKQPPKEPSEVPT
PKRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGR
PKKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQ
LPSGTGQLCSAPTAPTPSPGPPSPPPLAATPIF
HLCPHHHTTQHTSRCRAPMG*VGSSFPLASVPS
SPRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVV
GTLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPW
P*KGPKPHLILARPTPLPWQQQVWPMEGGAGPQ
DSPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSV
TGRQEG*VPYSLFTVATAPLPSAWDLSTYCGDG
DAVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRH
SAISPSSDGAPITRSSPCPLPTPLLLLPAPQGSG
SIFPLFTNYLWTVVLFFVQCSILRHPSLLLLPA
PNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*
PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGAT
SSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKI
SFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP
ASPWPPSRKRTALRSGAGAGRASSLRRSPAKCQH
LRDLGADQREAKTRVLPRPGKPPQLQEGNQGAD
PKNWRRRKRRASRRSPRRRSSDPCVPPAPHWRS
SFLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSS
TCALTTTLHSTPAAAGLPWAEWGAVFPWPQFPA
PPAHPRIHTCPPGQG*HPT*PHPAPAASPLPWW
WGHCSLGFWFGGALSAPSLFPLASHSGAWEGSPG
LKRGPSPISSWHALLHCPGSSRCGQWRGVLAPR
IPPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLR
LQEGRRGESPTPSSLWPQPPCPPPGI*VHIVVME
MQSLIVQVRPKSPVAAT*GGLGLLP*PYFASAT
QPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVL
VPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQR
QMFILIASCSAHDPLPQDTLCGEEGLGHGRLGD
RLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GP
PPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKY
PFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ
PALGLQAGKGRH*EAGPGQAAQAASEGAQRSAN
T*ETSGPTKGKQKQGCCQDPENHHNSRKETKGQT
QKTGEGGRGGHLAGVLGGGAVTHACRLLLTGGA
ASFWDWTALLRSHRPHPFPRPTITTASGRHPHL
PPVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQL
PPPTHAYTHALLDKANIPLSRTLHLLRPHSLGG
GDIALWAFGLGAPSLLLHCSLWLPIVGPGRVPL
ALKGAQAPSHPGTPYSTALAAAGVANGGGCWPPG
FPQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFG
YRKAGGVSPLLPLHCGHSPLALRLGSEYILW*W
RCSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPL
SHFPLLRWGTNNKELTLPAPNPPPAPPCPPRFW
FHFSSVHKLPLDSCVVFCSMFHSSTSVIAAATS
AKCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVT
DYPSPREGGALPLGCCSRVSKGAQIDHKGCRGH
LLPLFCWGGVAMICPSLGLPLWFPICSYLNKKN
ILFWTG

Sequence HMGA1.vbAug05

CAGGCTGGTCTCGAACTCCTGACCTCAAGCAGTCCTCCTGCTTTGGCATCCCAAAGTGCTGGGATTACAGCATCCCAGCCATCACTCTTCCACCTGCTCC
TTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCC
GCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGG
AAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGC
AGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGG
CCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGC
TGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAG
CCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCC
CTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAG
CAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAAC
TTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTT
GCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGG
GCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCC
CAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATG
TTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCT
TTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAG
CAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCT
CTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Alternative donor and acceptor

Frame 0

QAGLELLTSSSPPALASQSAGITASQPSLFHLL
LREGKMSESSSKSSQPLASKQEKDGTEKRGRGRP
RKQPPKEPSEVPTPKRPRGRPKGSKNKGAAKTR
KTTTTPGRKPRGRPKKLEKEEEEGISQESSEEE
Q*PMRAACSSLEEQLPSGTGQLCSAPTAPTPSPG
PPSPPPLAATPIFHLCPHHHTTQHTSRCRAPMG
*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSHL
AAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFTVP
SGFP*WGLGGFPWP*KGPKPHLILARPTPLPWQ
QQVWPMEGGAGPQDSPSQTVFVTTWGSLFILPQ
LP*SPY*VGQPPSVTGRQEG*VPYSLFTVATAPL
PSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLRW
AGAAPLTLLCFRHSAISPSSDGAPITRSSPCPL
PTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFVQC
SILRHPSLLLLPAPNVHPHCLLFCPRSPPPRYS
LWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE*
ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWGSL
SGFLFAVT*IKKISFSGLE

Frame 1

RLVSNS*PQAVLLLWHPKVLGLQHPSHHSSTCS
LEKGR*VSRARSPASPWPPSRKRTALRSGAGAG
RASSLRRSPAKCQHLRDLGADQREAKTRVLPRPG
KPPQLQEGNQGADPKNWRRRKRRASRRSPRRRS
SDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLPQ
AHHHHRLWPPPPSSTCALTTTLHSTPAAAGLPWA
EWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT*
PHPAPAASPLPWWWGHCSLGFWFGGALSAPSLF
PLASHSGAWEGSPGLKRGPSPISSWHALLHCPGS
SRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFPN
FPSPRTRLDSPLRLQEGRRGESPTPSSLWPQPP
CPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*GG
LGLLP*PYFASATQPFPPPQMGHQ*QGAHPARS
QPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLFN
VPFFDIRHCCCYQRQMFILIASCSAHDPLPQDTL
CGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQSE
QGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGAP
SLVSYLQLLE*KKYPFLDW

Frame 2

GWSRTPDLKQSSCFGIPKCWDYSIPAITLPPAP
*RREDE*VELEVQPALGLQAGKGRH*EAGPGQA
AQAASEGAQRSANT*ETSGPTKGKQKQGCCQDP
ENHHNSRKETKGQTQKTGEGGRGGHLAGVLGGGA
VTHACRLLLTGGAASFWDWTALLRSHRPHPFPR
PTITTASGRHPHLPPVPSPPHYTAHQPLQGSHG
LSGEQFSPGLSSQLPPPTHAYTHALLDKANIPLS
RTLHLLRPHSLGGGDIALWAFGLGAPSLLLHCS
LWLPIVGPGRVPLALKGAQAPSHPGTPYSTALA
AAGVANGGGCWPPGFPQPNCLCHHVGLTFHPSPT
SLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSPL
ALRLGSEYILW*WRCSHLLSR*GPRALWPPPEV
GWGCSPNPTLLPPLSHFPLLRWGTNNKELTLPAP
NPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCSM
FHSSTSVIAAATSAKCSSSLPPVLPTIPSPKIL
FVGKRGWGMAGWVTDYPSPREGGALPLGCCSRVS
KGAQIDHKGCRGHLLPLFCWGGVAMICPSLGLP
LWFPICSYLNKKNILFWTG

Sequence HMGA1.pAug05

GAGCGCGAGTTGTGCACCCGGCGGAGCCCGGAGGAGCCCGGCGCACCTCGCGCGGCGGCCGCTCCCGCTGGAGCCGGAGCCCGAGCCCGAGCCCGGGCCC
GGGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAA
GGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAG
GGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGG
AGGGCATCTC
GCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTG
CTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACA
CAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACA
TGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGT
TTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTC
ATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCA
CCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCC
CTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAG
GCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGG
CACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTA
CCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTG
TTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGG
GGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGG
GTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Alternative donor and acceptor

Frame 0

ERELCTRRSPEEPGAPRAAAAPAGAGARARARA
RASQPSLFHLLLREGKMSESSSKSSQPLASKQEK
DGTEKRGRGRPRKQPPKEPSEVPTPKRPRGRPK
GSKNKGAAKTRKTTTTPGRKPRGRPKKLEKEEE
EGIS
QESSEEEQ*PMRAACSSLEEQLPSGTGQLC
SAPTAPTPSPGPPSPPPLAATPIFHLCPHHHTT
QHTSRCRAPMG*VGSSFPLASVPSSPRPPTHTH
MPSWTRLTSHLAAPCTCCVPTPLVVGTLLSGLLV
WGRPLCSFTVPSGFP*WGLGGFPWP*KGPKPHL
ILARPTPLPWQQQVWPMEGGAGPQDSPSQTVFV
TTWGSLFILPQLP*SPY*VGQPPSVTGRQEG*VP
YSLFTVATAPLPSAWDLSTYCGDGDAVTYCPGE
AQEPCGRHLRWAGAAPLTLLCFRHSAISPSSDG
APITRSSPCPLPTPLLLLPAPQGSGSIFPLFTNY
LWTVVLFFVQCSILRHPSLLLLPAPNVHPHCLL
FCPRSPPPRYSLWGRGAGAWQAG*PTTPVPGKV
GPCP*DAAAE*ARGPKSTIKGVGATSSPCSVGEG
*P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 1

SASCAPGGARRSPAHLARRPLPLEPEPEPEPGP
GHPSHHSSTCSLEKGR*VSRARSPASPWPPSRK
RTALRSGAGAGRASSLRRSPAKCQHLRDLGADQR
EAKTRVLPRPGKPPQLQEGNQGADPKNWRRRKR
RAS
RRSPRRRSSDPCVPPAPHWRSSFLLGLDSF
APLPPPPPLPQAHHHHRLWPPPPSSTCALTTTLH
STPAAAGLPWAEWGAVFPWPQFPAPPAHPRIHT
CPPGQG*HPT*PHPAPAASPLPWWWGHCSLGFW
FGGALSAPSLFPLASHSGAWEGSPGLKRGPSPIS
SWHALLHCPGSSRCGQWRGVLAPRIPPAKLSLS
PRGAHFSSFPNFPSPRTRLDSPLRLQEGRRGES
PTPSSLWPQPPCPPPGI*VHIVVMEMQSLIVQVR
PKSPVAAT*GGLGLLP*PYFASATQPFPPPQMG
HQ*QGAHPARSQPPSCSSLPPKVLVPFFLCSQT
TSGQLCCFLFNVPFFDIRHCCCYQRQMFILIASC
SAHDPLPQDTLCGEEGLGHGRLGDRLPQSQGRW
GPAPRMLQQSEQGGPNRP*RV*GPPPPPVLLGR
GSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

ARVVHPAEPGGARRTSRGGRSRWSRSPSPSPGP
GIPAITLPPAP*RREDE*VELEVQPALGLQAGK
GRH*EAGPGQAAQAASEGAQRSANT*ETSGPTK
GKQKQGCCQDPENHHNSRKETKGQTQKTGEGGRG
GHL
AGVLGGGAVTHACRLLLTGGAASFWDWTAL
LRSHRPHPFPRPTITTASGRHPHLPPVPSPPHY
TAHQPLQGSHGLSGEQFSPGLSSQLPPPTHAYTH
ALLDKANIPLSRTLHLLRPHSLGGGDIALWAFG
LGAPSLLLHCSLWLPIVGPGRVPLALKGAQAPS
HPGTPYSTALAAAGVANGGGCWPPGFPQPNCLCH
HVGLTFHPSPTSLVPVLGWTAPFGYRKAGGVSP
LLPLHCGHSPLALRLGSEYILW*WRCSHLLSR*
GPRALWPPPEVGWGCSPNPTLLPPLSHFPLLRWG
TNNKELTLPAPNPPPAPPCPPRFWFHFSSVHKL
PLDSCVVFCSMFHSSTSVIAAATSAKCSSSLPP
VLPTIPSPKILFVGKRGWGMAGWVTDYPSPREGG
ALPLGCCSRVSKGAQIDHKGCRGHLLPLFCWGG
VAMICPSLGLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.jAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA
GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAA
ACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCC
TCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATG
GAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCA
CTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCT
GGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAG
CGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTG
ACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCC
ACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAA
TATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Alternative donor and acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP
SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRK
PRGRPKKLEKEEEEGISQESSEEEQ*PMRAACS
SLEEQLPSGTGQLCSAPTAPLPSAWDLSTYCGD
GDAVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRH
SAISPSSDGAPITRSSPCPLPTPLLLLPAPQGS
GSIFPLFTNYLWTVVLFFVQCSILRHPSLLLLP
APNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*
PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGA
TSSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKK
ISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP
AKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG
NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPAP
HWRSSFLLGLDSFAPLPQPPCPPPGI*VHIVVM
EMQSLIVQVRPKSPVAAT*GGLGLLP*PYFASA
TQPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVL
VPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQ
RQMFILIASCSAHDPLPQDTLCGEEGLGHGRLG
DRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GP
PPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KK
YPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ
RSANT*ETSGPTKGKQKQGCCQDPENHHNSRKE
TKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL
LTGGAASFWDWTALLRSHSPLALRLGSEYILW*W
RCSHLLSR*GPRALWPPPEVGWGCSPNPTLLPP
LSHFPLLRWGTNNKELTLPAPNPPPAPPCPPRF
WFHFSSVHKLPLDSCVVFCSMFHSSTSVIAAATS
AKCSSSLPPVLPTIPSPKILFVGKRGWGMAGWV
TDYPSPREGGALPLGCCSRVSKGAQIDHKGCRG
HLLPLFCWGGVAMICPSLGLPLWFPICSYLNKKN
ILFWTG

Sequence HMGA1.iAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA
GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAA
ACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCC
TCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCG
CCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGC
CTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCC
ACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGG
GAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGG
TGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGA
CAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACAT
ATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACT
TTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCC
CCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGC
TGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATG
GCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAG
GGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTT
GAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Alternative donor and acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP
SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRK
PRGRPKKLEKEEEEGISQESSEEEQ*PMRAACS
SLEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLA
ATPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPLA
SVPSSPRPPTHTHMPSWTRLTSHLAAPCTCCVP
TPLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGL
GGFPWP*KGPKPHLILARPTPLPWQQQVWPMEGG
AGPQDSPSQTVFVTTWGSLFILPQLP*SPY*VG
QPPSVTGRQEG*VPYSLFTVATAPLPSAWDLST
YCGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTLL
CFRHSAISPSSDGAPITRSSPCPLPTPLLLLPA
PQGSGSIFPLFTNYLWTVVLFFVQCSILRHPSL
LLLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGAW
QAG*PTTPVPGKVGPCP*DAAAE*ARGPKSTIK
GVGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT
*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP
AKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG
NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPAP
HWRSSFLLGLDSFAPLPPPPPLPQAHHHHRLWP
PPPSSTCALTTTLHSTPAAAGLPWAEWGAVFPW
PQFPAPPAHPRIHTCPPGQG*HPT*PHPAPAASP
LPWWWGHCSLGFWFGGALSAPSLFPLASHSGAW
EGSPGLKRGPSPISSWHALLHCPGSSRCGQWRG
VLAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRLD
SPLRLQEGRRGESPTPSSLWPQPPCPPPGI*VH
IVVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PY
FASATQPFPPPQMGHQ*QGAHPARSQPPSCSSLP
PKVLVPFFLCSQTTSGQLCCFLFNVPFFDIRHC
CCYQRQMFILIASCSAHDPLPQDTLCGEEGLGH
GRLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*R
V*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQLL
E*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ
RSANT*ETSGPTKGKQKQGCCQDPENHHNSRKE
TKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL
LTGGAASFWDWTALLRSHRPHPFPRPTITTASGR
HPHLPPVPSPPHYTAHQPLQGSHGLSGEQFSPG
LSSQLPPPTHAYTHALLDKANIPLSRTLHLLRP
HSLGGGDIALWAFGLGAPSLLLHCSLWLPIVGPG
RVPLALKGAQAPSHPGTPYSTALAAAGVANGGG
CWPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGW
TAPFGYRKAGGVSPLLPLHCGHSPLALRLGSEYI
LW*WRCSHLLSR*GPRALWPPPEVGWGCSPNPT
LLPPLSHFPLLRWGTNNKELTLPAPNPPPAPPC
PPRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVIA
AATSAKCSSSLPPVLPTIPSPKILFVGKRGWGM
AGWVTDYPSPREGGALPLGCCSRVSKGAQIDHK
GCRGHLLPLFCWGGVAMICPSLGLPLWFPICSYL
NKKNILFWTG

Sequence HMGA1.hAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT
ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG
TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC
AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC
GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT
CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCG
GGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAG
AAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGA
CTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCA
CCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCC
ACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCT
CTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCC
CAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAA
ACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGG
AGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTA
TTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCC
TCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTC
TGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCC
TCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTC
CCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTT
CTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACT
GGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Alternative donor and acceptor

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL
YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC
PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF
RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG
RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL
QAGKGRH*EAGPGQAAQAASEGAQRSANT*ETS
GPTKGKQKQGCCQDPENHHNSRKETKGQTQKTG
EGGRGGHLAGVLGGGAVTHACRLLLTGGAASFWD
WTALLRSHRPHPFPRPTITTASGRHPHLPPVPS
PPHYTAHQPLQGSHGLSGEQFSPGLSSQLPPPT
HAYTHALLDKANIPLSRTLHLLRPHSLGGGDIAL
WAFGLGAPSLLLHCSLWLPIVGPGRVPLALKGA
QAPSHPGTPYSTALAAAGVANGGGCWPPGFPQP
NCLCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAG
GVSPLLPLHCGHSPLALRLGSEYILW*WRCSHL
LSR*GPRALWPPPEVGWGCSPNPTLLPPLSHFP
LLRWGTNNKELTLPAPNPPPAPPCPPRFWFHFSS
VHKLPLDSCVVFCSMFHSSTSVIAAATSAKCSS
SLPPVLPTIPSPKILFVGKRGWGMAGWVTDYPS
PREGGALPLGCCSRVSKGAQIDHKGCRGHLLPLF
CWGGVAMICPSLGLPLWFPICSYLNKKNILFWT
G

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY
IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF
VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS
DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA
GGRARASQPSLFHLLLREGKMSESSSKSSQPLA
SKQEKDGTEKRGRGRPRKQPPKEPSEVPTPKRPR
GRPKGSKNKGAAKTRKTTTTPGRKPRGRPKKLE
KEEEEGISQESSEEEQ*PMRAACSSLEEQLPSG
TGQLCSAPTAPTPSPGPPSPPPLAATPIFHLCPH
HHTTQHTSRCRAPMG*VGSSFPLASVPSSPRPP
THTHMPSWTRLTSHLAAPCTCCVPTPLVVGTLL
SGLLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGP
KPHLILARPTPLPWQQQVWPMEGGAGPQDSPSQ
TVFVTTWGSLFILPQLP*SPY*VGQPPSVTGRQ
EG*VPYSLFTVATAPLPSAWDLSTYCGDGDAVTY
CPGEAQEPCGRHLRWAGAAPLTLLCFRHSAISP
SSDGAPITRSSPCPLPTPLLLLPAPQGSGSIFP
LFTNYLWTVVLFFVQCSILRHPSLLLLPAPNVHP
HCLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPV
PGKVGPCP*DAAAE*ARGPKSTIKGVGATSSPC
SVGEG*P*FVPAWGSLSGFLFAVT*IKKISFSGL
E

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI
SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL
SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF
QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP
AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP
PSRKRTALRSGAGAGRASSLRRSPAKCQHLRDL
GADQREAKTRVLPRPGKPPQLQEGNQGADPKNWR
RRKRRASRRSPRRRSSDPCVPPAPHWRSSFLLG
LDSFAPLPPPPPLPQAHHHHRLWPPPPSSTCAL
TTTLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHP
RIHTCPPGQG*HPT*PHPAPAASPLPWWWGHCS
LGFWFGGALSAPSLFPLASHSGAWEGSPGLKRG
PSPISSWHALLHCPGSSRCGQWRGVLAPRIPPAK
LSLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGR
RGESPTPSSLWPQPPCPPPGI*VHIVVMEMQSL
IVQVRPKSPVAAT*GGLGLLP*PYFASATQPFPP
PQMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFL
CSQTTSGQLCCFLFNVPFFDIRHCCCYQRQMFI
LIASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQS
QGRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPV
LLGRGSHDLSQPGAPSLVSYLQLLE*KKYPFLD
W

Sequence HMGA1.uAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG
CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTA
GGGAGTCAG
GAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCA
CAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCAT
GCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCA
CCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGA
GCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTG
CACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTC
CCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGT
GGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTC
CCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCC
TGGGATCTGAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGC
CACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTT
CTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACC
AGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGG
TGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGG
CCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAA
AATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Alternative donor and acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS
QPLASKQEKDGTEKRGRGRPRKQPPVSPGTALV
GSQ
EPSEVPTPKRPRGRPKGSKNKGAAKTRKTT
TTPGRKPRGRPKKLEKEEEEGISQESSEEEQ*PM
RAACSSLEEQLPSGTGQLCSAPTAPTPSPGPPS
PPPLAATPIFHLCPHHHTTQHTSRCRAPMG*VG
SSFPLASVPSSPRPPTHTHMPSWTRLTSHLAAPC
TCCVPTPLVVGTLLSGLLVWGRPLCSFTVPSGF
P*WGLGGFPWP*KGPKPHLILARPTPLPWQQQV
WPMEGGAGPQDSPSQTVFVTTWGSLFILPQLP*S
PY*VGQPPSVTGRQEG*VPYSLFTVATAPLPSA
WDLSHLLSR*GPRALWPPPEVGWGCSPNPTLLP
PLSHFPLLRWGTNNKELTLPAPNPPPAPPCPPRF
WFHFSSVHKLPLDSCVVFCSMFHSSTSVIAAAT
SAKCSSSLPPVLPTIPSPKILFVGKRGWGMAGW
VTDYPSPREGGALPLGCCSRVSKGAQIDHKGCRG
HLLPLFCWGGVAMICPSLGLPLWFPICSYLNKK
NILFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP
ASPWPPSRKRTALRSGAGAGRASSLR*VPGQRW*
GVR
SPAKCQHLRDLGADQREAKTRVLPRPGKPP
QLQEGNQGADPKNWRRRKRRASRRSPRRRSSDP
CVPPAPHWRSSFLLGLDSFAPLPPPPPLPQAHHH
HRLWPPPPSSTCALTTTLHSTPAAAGLPWAEWG
AVFPWPQFPAPPAHPRIHTCPPGQG*HPT*PHP
APAASPLPWWWGHCSLGFWFGGALSAPSLFPLAS
HSGAWEGSPGLKRGPSPISSWHALLHCPGSSRC
GQWRGVLAPRIPPAKLSLSPRGAHFSSFPNFPS
PRTRLDSPLRLQEGRRGESPTPSSLWPQPPCPPP
GI*VTYCPGEAQEPCGRHLRWAGAAPLTLLCFR
HSAISPSSDGAPITRSSPCPLPTPLLLLPAPQG
SGSIFPLFTNYLWTVVLFFVQCSILRHPSLLLLP
APNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG
*PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVG
ATSSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKK
ISFSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ
PALGLQAGKGRH*EAGPGQAAQAASGESRDSAG
RES
GAQRSANT*ETSGPTKGKQKQGCCQDPENHH
NSRKETKGQTQKTGEGGRGGHLAGVLGGGAVTH
ACRLLLTGGAASFWDWTALLRSHRPHPFPRPTI
TTASGRHPHLPPVPSPPHYTAHQPLQGSHGLSGE
QFSPGLSSQLPPPTHAYTHALLDKANIPLSRTL
HLLRPHSLGGGDIALWAFGLGAPSLLLHCSLWL
PIVGPGRVPLALKGAQAPSHPGTPYSTALAAAGV
ANGGGCWPPGFPQPNCLCHHVGLTFHPSPTSLV
PVLGWTAPFGYRKAGGVSPLLPLHCGHSPLALR
LGSESLIVQVRPKSPVAAT*GGLGLLP*PYFASA
TQPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKV
LVPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCY
QRQMFILIASCSAHDPLPQDTLCGEEGLGHGRLG
DRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*G
PPPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*K
KYPFLDW

Sequence HMGA1.gAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG
GGACAGCGCTGGTAGGGAGTCAG
GAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGAC
CCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAG
GAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCC
CAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCAT
GGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCAC
TTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTG
TTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTG
GCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCC
CAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCC
CCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAG
GTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCG
CTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTC
AATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATA
CTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAG
TGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCT
CCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Alternative donor and acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP
GTALVGSQ
EPSEVPTPKRPRGRPKGSKNKGAAKT
RKTTTTPGRKPRGRPKKLEKEEEEGISQESSEE
EQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS
PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAPM
G*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSH
LAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT
VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLPW
QQQVWPMEGGAGPQDSPSQTVFVTTWGSLFILP
QLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA
PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLR
WAGAAPLTLLCFRHSAISPSSDGAPITRSSPCP
LPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV
QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPRY
SLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE
*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG
SLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP
GQRW*GVR
SPAKCQHLRDLGADQREAKTRVLPR
PGKPPQLQEGNQGADPKNWRRRKRRASRRSPRRR
SSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLP
QAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP
WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT
*PHPAPAASPLPWWWGHCSLGFWFGGALSAPSL
FPLASHSGAWEGSPGLKRGPSPISSWHALLHCP
GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFP
NFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQP
PCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*
GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPAR
SQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLF
NVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD
TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQS
EQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGA
PSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRES
GAQRSANT*ETSGPTKGKQKQGCCQD
PENHHNSRKETKGQTQKTGEGGRGGHLAGVLGG
GAVTHACRLLLTGGAASFWDWTALLRSHRPHPFP
RPTITTASGRHPHLPPVPSPPHYTAHQPLQGSH
GLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP
LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLHC
SLWLPIVGPGRVPLALKGAQAPSHPGTPYSTAL
AAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS
PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSP
LALRLGSEYILW*WRCSHLLSR*GPRALWPPPE
VGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP
APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCS
MFHSSTSVIAAATSAKCSSSLPPVLPTIPSPKI
LFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR
VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLGL
PLWFPICSYLNKKNILFWTG

Sequence HMGA1.dAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT
ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG
TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC
AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC
GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT
CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGGAGCC
CAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGG
AAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCT
CCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGC
CGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTG
GCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCC
CCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCT
GGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGG
GGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTG
GACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTAC
ATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTA
CTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTG
CCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATT
GCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCA
TGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAA
AGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTAC
TTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Alternative donor and acceptor

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL
YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC
PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF
RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG
RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL
QAGKGRH*EAGPGQAAQAASGESRDSAGRESGA
QRSANT*ETSGPTKGKQKQGCCQDPENHHNSRK
ETKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL
LTGGAASFWDWTALLRSHRPHPFPRPTITTASG
RHPHLPPVPSPPHYTAHQPLQGSHGLSGEQFSP
GLSSQLPPPTHAYTHALLDKANIPLSRTLHLLRP
HSLGGGDIALWAFGLGAPSLLLHCSLWLPIVGP
GRVPLALKGAQAPSHPGTPYSTALAAAGVANGG
GCWPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGW
TAPFGYRKAGGVSPLLPLHCGHSPLALRLGSEY
ILW*WRCSHLLSR*GPRALWPPPEVGWGCSPNP
TLLPPLSHFPLLRWGTNNKELTLPAPNPPPAPPC
PPRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVI
AAATSAKCSSSLPPVLPTIPSPKILFVGKRGWG
MAGWVTDYPSPREGGALPLGCCSRVSKGAQIDHK
GCRGHLLPLFCWGGVAMICPSLGLPLWFPICSY
LNKKNILFWTG

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY
IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF
VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS
DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA
GGRARASQPSLFHLLLREGKMSESSSKSSQPLA
SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQEP
SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGR
KPRGRPKKLEKEEEEGISQESSEEEQ*PMRAAC
SSLEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLA
ATPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPL
ASVPSSPRPPTHTHMPSWTRLTSHLAAPCTCCV
PTPLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGL
GGFPWP*KGPKPHLILARPTPLPWQQQVWPMEG
GAGPQDSPSQTVFVTTWGSLFILPQLP*SPY*V
GQPPSVTGRQEG*VPYSLFTVATAPLPSAWDLST
YCGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTL
LCFRHSAISPSSDGAPITRSSPCPLPTPLLLLP
APQGSGSIFPLFTNYLWTVVLFFVQCSILRHPSL
LLLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGA
WQAG*PTTPVPGKVGPCP*DAAAE*ARGPKSTI
KGVGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT
*IKKISFSGLE

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI
SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL
SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF
QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP
AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP
PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRS
PAKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG
NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPA
PHWRSSFLLGLDSFAPLPPPPPLPQAHHHHRLW
PPPPSSTCALTTTLHSTPAAAGLPWAEWGAVFPW
PQFPAPPAHPRIHTCPPGQG*HPT*PHPAPAAS
PLPWWWGHCSLGFWFGGALSAPSLFPLASHSGA
WEGSPGLKRGPSPISSWHALLHCPGSSRCGQWRG
VLAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRL
DSPLRLQEGRRGESPTPSSLWPQPPCPPPGI*V
HIVVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PY
FASATQPFPPPQMGHQ*QGAHPARSQPPSCSSL
PPKVLVPFFLCSQTTSGQLCCFLFNVPFFDIRH
CCCYQRQMFILIASCSAHDPLPQDTLCGEEGLGH
GRLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*
RV*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQL
LE*KKYPFLDW

Sequence HMGA1.rAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT
ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG
TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC
AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC
GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT
CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCGGGACAGCGCTGGTAGGGAGTCAGGAGCC
CAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCG
GAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCC
CTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGC
TCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACA
TCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCT
TCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACT
GCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATC
CTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCA
CAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCA
CCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCC
TGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTT
TTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCC
AAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCA
GCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCT
GGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Alternative donor and acceptor

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL
YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC
PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF
RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG
RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL
QAGKGRH*EAGPGQAAQAASGESRDSAGRESGA
QRSANT*ETSGPTKGKQKQG*EGGRGGHLAGVL
GGGAVTHACRLLLTGGAASFWDWTALLRSHRPHP
FPRPTITTASGRHPHLPPVPSPPHYTAHQPLQG
SHGLSGEQFSPGLSSQLPPPTHAYTHALLDKAN
IPLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLL
HCSLWLPIVGPGRVPLALKGAQAPSHPGTPYST
ALAAAGVANGGGCWPPGFPQPNCLCHHVGLTFH
PSPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGH
SPLALRLGSEYILW*WRCSHLLSR*GPRALWPP
PEVGWGCSPNPTLLPPLSHFPLLRWGTNNKELT
LPAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVF
CSMFHSSTSVIAAATSAKCSSSLPPVLPTIPSP
KILFVGKRGWGMAGWVTDYPSPREGGALPLGCC
SRVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSL
GLPLWFPICSYLNKKNILFWTG

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY
IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF
VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS
DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA
GGRARASQPSLFHLLLREGKMSESSSKSSQPLA
SKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQEP
SEVPTPKRPRGRPKGSKNKGEKEEEEGISQESS
EEEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPT
PSPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRA
PMG*VGSSFPLASVPSSPRPPTHTHMPSWTRLT
SHLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCS
FTVPSGFP*WGLGGFPWP*KGPKPHLILARPTPL
PWQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFI
LPQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVA
TAPLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRH
LRWAGAAPLTLLCFRHSAISPSSDGAPITRSSP
CPLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLF
FVQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPP
RYSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAA
AE*ARGPKSTIKGVGATSSPCSVGEG*P*FVPA
WGSLSGFLFAVT*IKKISFSGLE

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI
SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL
SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF
QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP
AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP
PSRKRTALRSGAGAGRASSLR*VPGQRW*GVRS
PAKCQHLRDLGADQREAKTRVRRRKRRASRRSPR
RRSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPP
LPQAHHHHRLWPPPPSSTCALTTTLHSTPAAAG
LPWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*H
PT*PHPAPAASPLPWWWGHCSLGFWFGGALSAP
SLFPLASHSGAWEGSPGLKRGPSPISSWHALLH
CPGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSS
FPNFPSPRTRLDSPLRLQEGRRGESPTPSSLWP
QPPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAA
T*GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHP
ARSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCF
LFNVPFFDIRHCCCYQRQMFILIASCSAHDPLP
QDTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQ
QSEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQP
GAPSLVSYLQLLE*KKYPFLDW

Sequence HMGA1.oAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA
TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC
CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG
AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGTGAGTCCCG
GGACAGCGCTGGTAGGGAGTCAG
GAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGA
AGAGGAGGGCATCTC
GCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAG
CTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACA
CTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATAC
ACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTT
TTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCC
ATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTT
TGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGA
GTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAG
GTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGA
TGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACA
AACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCC
TCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAA
GGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGG
GAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG
  • HMGA1.au.0.f (forward primer)
  • HMGA1.u.r.18 (reverse primer)
  • Alternative donor and acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA
ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP
GNPERAPQAGGRARASQPSLFHLLLREGKMSES
SSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSP
GTALVGSQ
EPSEVPTPKRPRGRPKGSKNKGEKEE
EEGIS
QESSEEEQ*PMRAACSSLEEQLPSGTGQ
LCSAPTAPTPSPGPPSPPPLAATPIFHLCPHHH
TTQHTSRCRAPMG*VGSSFPLASVPSSPRPPTHT
HMPSWTRLTSHLAAPCTCCVPTPLVVGTLLSGL
LVWGRPLCSFTVPSGFP*WGLGGFPWP*KGPKP
HLILARPTPLPWQQQVWPMEGGAGPQDSPSQTVF
VTTWGSLFILPQLP*SPY*VGQPPSVTGRQEG*
VPYSLFTVATAPLPSAWDLSTYCGDGDAVTYCP
GEAQEPCGRHLRWAGAAPLTLLCFRHSAISPSSD
GAPITRSSPCPLPTPLLLLPAPQGSGSIFPLFT
NYLWTVVLFFVQCSILRHPSLLLLPAPNVHPHC
LLFCPRSPPPRYSLWGRGAGAWQAG*PTTPVPGK
VGPCP*DAAAE*ARGPKSTIKGVGATSSPCSVG
EG*P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL
FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL
PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR
ARSPASPWPPSRKRTALRSGAGAGRASSLR*VP
GQRW*GVR
SPAKCQHLRDLGADQREAKTRVRRR
KRRAS
RRSPRRRSSDPCVPPAPHWRSSFLLGLDS
FAPLPPPPPLPQAHHHHRLWPPPPSSTCALTTT
LHSTPAAAGLPWAEWGAVFPWPQFPAPPAHPRI
HTCPPGQG*HPT*PHPAPAASPLPWWWGHCSLGF
WFGGALSAPSLFPLASHSGAWEGSPGLKRGPSP
ISSWHALLHCPGSSRCGQWRGVLAPRIPPAKLS
LSPRGAHFSSFPNFPSPRTRLDSPLRLQEGRRGE
SPTPSSLWPQPPCPPPGI*VHIVVMEMQSLIVQ
VRPKSPVAAT*GGLGLLP*PYFASATQPFPPPQ
MGHQ*QGAHPARSQPPSCSSLPPKVLVPFFLCSQ
TTSGQLCCFLFNVPFFDIRHCCCYQRQMFILIA
SCSAHDPLPQDTLCGEEGLGHGRLGDRLPQSQG
RWGPAPRMLQQSEQGGPNRP*RV*GPPPPPVLLG
RGSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY
FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS
RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V
ELEVQPALGLQAGKGRH*EAGPGQAAQAASGESR
DSAGRES
GAQRSANT*ETSGPTKGKQKQG*EGG
RGGHL
AGVLGGGAVTHACRLLLTGGAASFWDWT
ALLRSHRPHPFPRPTITTASGRHPHLPPVPSPPH
YTAHQPLQGSHGLSGEQFSPGLSSQLPPPTHAY
THALLDKANIPLSRTLHLLRPHSLGGGDIALWA
FGLGAPSLLLHCSLWLPIVGPGRVPLALKGAQAP
SHPGTPYSTALAAAGVANGGGCWPPGFPQPNCL
CHHVGLTFHPSPTSLVPVLGWTAPFGYRKAGGV
SPLLPLHCGHSPLALRLGSEYILW*WRCSHLLSR
*GPRALWPPPEVGWGCSPNPTLLPPLSHFPLLR
WGTNNKELTLPAPNPPPAPPCPPRFWFHFSSVH
KLPLDSCVVFCSMFHSSTSVIAAATSAKCSSSLP
PVLPTIPSPKILFVGKRGWGMAGWVTDYPSPRE
GGALPLGCCSRVSKGAQIDHKGCRGHLLPLFCW
GGVAMICPSLGLPLWFPICSYLNKKNILFWTG

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