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Sequences

Sequence HMGA1.vkAug05

GTTGGAACTAGTCCCTTTGACTCCCCGTTTCTATTTTATTTTCTTCTGGGGCTTTATTTTGCGGGGAGGGGACGGAGGGAGGGGGCTTTCTTCCTCTCCC CCGCGCCCGCGCGCGCCAGGTTTCCTGCCCCAAGGCGGGCTTGGGTGCCCCCTCTGGCAGGAAGTGGGGCCGGGTGCACCCGCGTGGCGGTGCATGCGGT CGGGGTGCGCTGCTTTCCCGCGCCTGCAGGAGCGGCCTGCGCCCCTCCCCCCGCGCGCCCTGGCCCCGGGTCCCGCCGGCCGGCCTGCAACGGCCGGGCG CAGACACTTGCAGAGTCACCCTCGGGCCGGGTCTGGAGCCTGATGCCTCGGGGGTTTAGCCCTAGCCGCTAGGCGGCATCCCAGCCATCACTCTTCCACC TGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGG CAGGCCGCGCAAGCAGCCTCCGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACC CGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGG AGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCC AGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATG GGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACT TAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGT TCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGG CAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCC AACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCC CTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGG TGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGC TCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCA ATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATAC TCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGT GAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTC CCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

VGTSPFDSPFLFYFLLGLYFAGRGRREGAFFLS PAPARARFPAPRRAWVPPLAGSGAGCTRVAVHAV GVRCFPAPAGAACAPPPARPGPGSRRPACNGRA QTLAESPSGRVWSLMPRGFSPSR*AASQPSLFH LLLREGKMSESSSKSSQPLASKQEKDGTEKRGRG RPRKQPPEPSEVPTPKRPRGRPKGSKNKGAAKT RKTTTTPGRKPRGRPKKLEKEEEEGISQESSEE EQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPSP GPPSPPPLAATPIFHLCPHHHTTQHTSRCRAPM G*VGSSFPLASVPSSPRPPTHTHMPSWTRLTSH LAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFTV PSGFP*WGLGGFPWP*KGPKPHLILARPTPLPW QQQVWPMEGGAGPQDSPSQTVFVTTWGSLFILP QLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATAP LPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLR WAGAAPLTLLCFRHSAISPSSDGAPITRSSPCP LPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFVQ CSILRHPSLLLLPAPNVHPHCLLFCPRSPPPRY SLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE *ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWGS LSGFLFAVT*IKKISFSGLE

Frame 1

LELVPLTPRFYFIFFWGFILRGGDGGRGLSSSP PRPRAPGFLPQGGLGCPLWQEVGPGAPAWRCMR SGCAAFPRLQERPAPLPPRALAPGPAGRPATAGR RHLQSHPRAGSGA*CLGGLALAARRHPSHHSST CSLEKGR*VSRARSPASPWPPSRKRTALRSGAG AGRASSLRSPAKCQHLRDLGADQREAKTRVLPRP GKPPQLQEGNQGADPKNWRRRKRRASRRSPRRR SSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLP QAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLPW AEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT *PHPAPAASPLPWWWGHCSLGFWFGGALSAPSL FPLASHSGAWEGSPGLKRGPSPISSWHALLHCPG SSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFP NFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQP PCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*G GLGLLP*PYFASATQPFPPPQMGHQ*QGAHPAR SQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLF NVPFFDIRHCCCYQRQMFILIASCSAHDPLPQDT LCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQS EQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGA PSLVSYLQLLE*KKYPFLDW

Frame 2

WN*SL*LPVSILFSSGALFCGEGTEGGGFLPLP RARARQVSCPKAGLGAPSGRKWGRVHPRGGACG RGALLSRACRSGLRPSPRAPWPRVPPAGLQRPG ADTCRVTLGPGLEPDASGV*P*PLGGIPAITLPP AP*RREDE*VELEVQPALGLQAGKGRH*EAGPG QAAQAASGAQRSANT*ETSGPTKGKQKQGCCQD PENHHNSRKETKGQTQKTGEGGRGGHLAGVLGGG AVTHACRLLLTGGAASFWDWTALLRSHRPHPFP RPTITTASGRHPHLPPVPSPPHYTAHQPLQGSH GLSGEQFSPGLSSQLPPPTHAYTHALLDKANIPL SRTLHLLRPHSLGGGDIALWAFGLGAPSLLLHC SLWLPIVGPGRVPLALKGAQAPSHPGTPYSTAL AAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPSP TSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSP LALRLGSEYILW*WRCSHLLSR*GPRALWPPPE VGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLPA PNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCS MFHSSTSVIAAATSAKCSSSLPPVLPTIPSPKI LFVGKRGWGMAGWVTDYPSPREGGALPLGCCSRV SKGAQIDHKGCRGHLLPLFCWGGVAMICPSLGL PLWFPICSYLNKKNILFWTG

Sequence HMGA1.veAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGAGCCCAGCGAAGTGCCAACACCT AAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCA AAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCT TCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCAC CTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCC CCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGG GACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCT TAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATT CCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTAC AGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGAT GCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAG CCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTC CATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCA AATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGA CTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTC CTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCC TTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPEPSEVPTP KRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRP KKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQL PSGTGQLCSAPTAPTPSPGPPSPPPLAATPIFH LCPHHHTTQHTSRCRAPMG*VGSSFPLASVPSS PRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVVG TLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPWP *KGPKPHLILARPTPLPWQQQVWPMEGGAGPQD SPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSVT GRQEG*VPYSLFTVATAPLPSAWDLSTYCGDGD AVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRHS AISPSSDGAPITRSSPCPLPTPLLLLPAPQGSGS IFPLFTNYLWTVVLFFVQCSILRHPSLLLLPAP NVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*P TTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGATS SPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKIS FSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLRSPAKCQHL RDLGADQREAKTRVLPRPGKPPQLQEGNQGADP KNWRRRKRRASRRSPRRRSSDPCVPPAPHWRSS FLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSST CALTTTLHSTPAAAGLPWAEWGAVFPWPQFPAP PAHPRIHTCPPGQG*HPT*PHPAPAASPLPWWW GHCSLGFWFGGALSAPSLFPLASHSGAWEGSPGL KRGPSPISSWHALLHCPGSSRCGQWRGVLAPRI PPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLRL QEGRRGESPTPSSLWPQPPCPPPGI*VHIVVMEM QSLIVQVRPKSPVAAT*GGLGLLP*PYFASATQ PFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVLV PFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQRQ MFILIASCSAHDPLPQDTLCGEEGLGHGRLGDR LPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GPP PPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKYP FLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASGAQRSANT *ETSGPTKGKQKQGCCQDPENHHNSRKETKGQTQ KTGEGGRGGHLAGVLGGGAVTHACRLLLTGGAA SFWDWTALLRSHRPHPFPRPTITTASGRHPHLP PVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQLP PPTHAYTHALLDKANIPLSRTLHLLRPHSLGGG DIALWAFGLGAPSLLLHCSLWLPIVGPGRVPLA LKGAQAPSHPGTPYSTALAAAGVANGGGCWPPGF PQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFGY RKAGGVSPLLPLHCGHSPLALRLGSEYILW*WR CSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPLS HFPLLRWGTNNKELTLPAPNPPPAPPCPPRFWF HFSSVHKLPLDSCVVFCSMFHSSTSVIAAATSA KCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVTD YPSPREGGALPLGCCSRVSKGAQIDHKGCRGHL LPLFCWGGVAMICPSLGLPLWFPICSYLNKKNI LFWTG

Sequence HMGA1.lAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGGAGCCCAGCG AAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACC AAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCA CTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCA CCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTC AGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACT CCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAG GGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGC TGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAG CCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATT GTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTG CTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCC CAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGC TGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCA GGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGT GTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAA TAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPEPS EVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRKP RGRPKKLEKEEEEGISQESSEEEQ*PMRAACSS LEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLAA TPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPLAS VPSSPRPPTHTHMPSWTRLTSHLAAPCTCCVPT PLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGLG GFPWP*KGPKPHLILARPTPLPWQQQVWPMEGGA GPQDSPSQTVFVTTWGSLFILPQLP*SPY*VGQ PPSVTGRQEG*VPYSLFTVATAPLPSAWDLSTY CGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTLLC FRHSAISPSSDGAPITRSSPCPLPTPLLLLPAP QGSGSIFPLFTNYLWTVVLFFVQCSILRHPSLL LLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGAWQ AG*PTTPVPGKVGPCP*DAAAE*ARGPKSTIKG VGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT* IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLRSPA KCQHLRDLGADQREAKTRVLPRPGKPPQLQEGN QGADPKNWRRRKRRASRRSPRRRSSDPCVPPAPH WRSSFLLGLDSFAPLPPPPPLPQAHHHHRLWPP PPSSTCALTTTLHSTPAAAGLPWAEWGAVFPWP QFPAPPAHPRIHTCPPGQG*HPT*PHPAPAASPL PWWWGHCSLGFWFGGALSAPSLFPLASHSGAWE GSPGLKRGPSPISSWHALLHCPGSSRCGQWRGV LAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRLDS PLRLQEGRRGESPTPSSLWPQPPCPPPGI*VHI VVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PYF ASATQPFPPPQMGHQ*QGAHPARSQPPSCSSLPP KVLVPFFLCSQTTSGQLCCFLFNVPFFDIRHCC CYQRQMFILIASCSAHDPLPQDTLCGEEGLGHG RLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV *GPPPPPVLLGRGSHDLSQPGAPSLVSYLQLLE *KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASGAQR SANT*ETSGPTKGKQKQGCCQDPENHHNSRKET KGQTQKTGEGGRGGHLAGVLGGGAVTHACRLLL TGGAASFWDWTALLRSHRPHPFPRPTITTASGRH PHLPPVPSPPHYTAHQPLQGSHGLSGEQFSPGL SSQLPPPTHAYTHALLDKANIPLSRTLHLLRPH SLGGGDIALWAFGLGAPSLLLHCSLWLPIVGPGR VPLALKGAQAPSHPGTPYSTALAAAGVANGGGC WPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGWT APFGYRKAGGVSPLLPLHCGHSPLALRLGSEYIL W*WRCSHLLSR*GPRALWPPPEVGWGCSPNPTL LPPLSHFPLLRWGTNNKELTLPAPNPPPAPPCP PRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVIAA ATSAKCSSSLPPVLPTIPSPKILFVGKRGWGMA GWVTDYPSPREGGALPLGCCSRVSKGAQIDHKG CRGHLLPLFCWGGVAMICPSLGLPLWFPICSYLN KKNILFWTG

Sequence HMGA1.qAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGA GGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCT TCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTC CCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATC CCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTC ACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGC CCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCT TCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACA GCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACC TGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTG CCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTT GTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAA GATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGC AGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGG GGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP SEVPTPKRPRGRPKGSKNKGEKEEEEGISQESSE EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTP SPGPPSPPPLAATPIFHLCPHHHTTQHTSRCRA PMG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSF TVPSGFP*WGLGGFPWP*KGPKPHLILARPTPL PWQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVAT APLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRH LRWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFF VQCSILRHPSLLLLPAPNVHPHCLLFCPRSPPP RYSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAW GSLSGFLFAVT*IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP AKCQHLRDLGADQREAKTRVRRRKRRASRRSPR RRSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGL PWAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*H PT*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS LFPLASHSGAWEGSPGLKRGPSPISSWHALLHC PGSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSS FPNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT *GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHP ARSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQ DTLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQ QSEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG APSLVSYLQLLE*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ RSANT*ETSGPTKGKQKQG*EGGRGGHLAGVLG GGAVTHACRLLLTGGAASFWDWTALLRSHRPHP FPRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANI PLSRTLHLLRPHSLGGGDIALWAFGLGAPSLLL HCSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHP SPTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGH SPLALRLGSEYILW*WRCSHLLSR*GPRALWPPP EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTL PAPNPPPAPPCPPRFWFHFSSVHKLPLDSCVVF CSMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCS RVSKGAQIDHKGCRGHLLPLFCWGGVAMICPSL GLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.vlAug05

GGGTCCCCAGCAGCAAGAGGTGGGGGGAGGCACCAGATGGTATGAGAGCTTCCAGGGAGACCCGCCAAGATCTCCAGGCAGGCCGGGCCAGGCTCTCTGG GATTCCGGACTGGCTTCGCCCAACTGGAACCCCCTCGATGAGGGGGCCCCAGGCAGACCTTATATGAGCATCCCAGCCATCACTCTTCCACCTGCTCCTT AGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGC GCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAA AACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAG TGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCC CACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTG AGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCC GCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCT CTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCA GCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTT CCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGC CCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGC TGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCA ACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTT CCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTT GTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCA AGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCT CTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

GSPAARGGGRHQMV*ELPGRPAKISRQAGPGSL GFRTGFAQLEPPR*GGPRQTLYEHPSHHSSTCSL EKGR*VSRARSPASPWPPSRKRTALRSGAGAGR ASSLRRSPAKCQHLRDLGADQREAKTRVLPRPG KPPQLQEGNQGADPKNWRRRKRRASRRSPRRRSS DPCVPPAPHWRSSFLLGLDSFAPLPPPPPLPQA HHHHRLWPPPPSSTCALTTTLHSTPAAAGLPWA EWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT*P HPAPAASPLPWWWGHCSLGFWFGGALSAPSLFP LASHSGAWEGSPGLKRGPSPISSWHALLHCPGS SRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFPNF PSPRTRLDSPLRLQEGRRGESPTPSSLWPQPPC PPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*GG LGLLP*PYFASATQPFPPPQMGHQ*QGAHPARSQ PPSCSSLPPKVLVPFFLCSQTTSGQLCCFLFNV PFFDIRHCCCYQRQMFILIASCSAHDPLPQDTL CGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQSEQ GGPNRP*RV*GPPPPPVLLGRGSHDLSQPGAPS LVSYLQLLE*KKYPFLDW

Frame 1

GPQQQEVGGGTRWYESFQGDPPRSPGRPGQALW DSGLASPNWNPLDEGAPGRPYMSIPAITLPPAP *RREDE*VELEVQPALGLQAGKGRH*EAGPGQAA QAASEGAQRSANT*ETSGPTKGKQKQGCCQDPE NHHNSRKETKGQTQKTGEGGRGGHLAGVLGGGA VTHACRLLLTGGAASFWDWTALLRSHRPHPFPRP TITTASGRHPHLPPVPSPPHYTAHQPLQGSHGL SGEQFSPGLSSQLPPPTHAYTHALLDKANIPLS RTLHLLRPHSLGGGDIALWAFGLGAPSLLLHCSL WLPIVGPGRVPLALKGAQAPSHPGTPYSTALAA AGVANGGGCWPPGFPQPNCLCHHVGLTFHPSPT SLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSPLA LRLGSEYILW*WRCSHLLSR*GPRALWPPPEVG WGCSPNPTLLPPLSHFPLLRWGTNNKELTLPAP NPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCSMF HSSTSVIAAATSAKCSSSLPPVLPTIPSPKILF VGKRGWGMAGWVTDYPSPREGGALPLGCCSRVS KGAQIDHKGCRGHLLPLFCWGGVAMICPSLGLPL WFPICSYLNKKNILFWTG

Frame 2

VPSSKRWGEAPDGMRASRETRQDLQAGRARLSG IPDWLRPTGTPSMRGPQADLI*ASQPSLFHLLL REGKMSESSSKSSQPLASKQEKDGTEKRGRGRP RKQPPKEPSEVPTPKRPRGRPKGSKNKGAAKTRK TTTTPGRKPRGRPKKLEKEEEEGISQESSEEEQ *PMRAACSSLEEQLPSGTGQLCSAPTAPTPSPG PPSPPPLAATPIFHLCPHHHTTQHTSRCRAPMG* VGSSFPLASVPSSPRPPTHTHMPSWTRLTSHLA APCTCCVPTPLVVGTLLSGLLVWGRPLCSFTVP SGFP*WGLGGFPWP*KGPKPHLILARPTPLPWQQ QVWPMEGGAGPQDSPSQTVFVTTWGSLFILPQL P*SPY*VGQPPSVTGRQEG*VPYSLFTVATAPL PSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLRWA GAAPLTLLCFRHSAISPSSDGAPITRSSPCPLP TPLLLLPAPQGSGSIFPLFTNYLWTVVLFFVQC SILRHPSLLLLPAPNVHPHCLLFCPRSPPPRYSL WGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE*A RGPKSTIKGVGATSSPCSVGEG*P*FVPAWGSL SGFLFAVT*IKKISFSGLE

Sequence HMGA1.vjAug05

GTTGGAACTAGTCCCTTTGACTCCCCGTTTCTATTTTATTTTCTTCTGGGGCTTTATTTTGCGGGGAGGGGACGGAGGGAGGGGGCTTTCTTCCTCTCCC CCGCGCCCGCGCGCGCCAGGTTTCCTGCCCCAAGGCGGGCTTGGGTGCCCCCTCTGGCAGGAAGTGGGGCCGGGTGCACCCGCGTGGCGGTGCATGCGGT CGGGGTGCGCTGCTTTCCCGCGCCTGCAGGAGCGGCCTGCGCCCCTCCCCCCGCGCGCCCTGGCCCCGGGTCCCGCCGGCCGGCCTGCAACGGCCGGGCG CAGACACTTGCAGAGTCACCCTCGGGCCGGGTCTGGAGCCTGATGCCTCGGGGGTTTAGCCCTAGCCGCTAGGCGGCATCCCAGCCATCACTCTTCCACC TGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGG CAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAG ACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGG AGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTC CCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCC ATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCC ACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCAC TGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCC TGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTC CCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGC CCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTG AGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCC CGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGT TCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGA TACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAG AGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGG CTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

VGTSPFDSPFLFYFLLGLYFAGRGRREGAFFLS PAPARARFPAPRRAWVPPLAGSGAGCTRVAVHAV GVRCFPAPAGAACAPPPARPGPGSRRPACNGRA QTLAESPSGRVWSLMPRGFSPSR*AASQPSLFH LLLREGKMSESSSKSSQPLASKQEKDGTEKRGRG RPRKQPPKEPSEVPTPKRPRGRPKGSKNKGAAK TRKTTTTPGRKPRGRPKKLEKEEEEGISQESSE EEQ*PMRAACSSLEEQLPSGTGQLCSAPTAPTPS PGPPSPPPLAATPIFHLCPHHHTTQHTSRCRAP MG*VGSSFPLASVPSSPRPPTHTHMPSWTRLTS HLAAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFT VPSGFP*WGLGGFPWP*KGPKPHLILARPTPLP WQQQVWPMEGGAGPQDSPSQTVFVTTWGSLFIL PQLP*SPY*VGQPPSVTGRQEG*VPYSLFTVATA PLPSAWDLSTYCGDGDAVTYCPGEAQEPCGRHL RWAGAAPLTLLCFRHSAISPSSDGAPITRSSPC PLPTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFV QCSILRHPSLLLLPAPNVHPHCLLFCPRSPPPR YSLWGRGAGAWQAG*PTTPVPGKVGPCP*DAAA E*ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWG SLSGFLFAVT*IKKISFSGLE

Frame 1

LELVPLTPRFYFIFFWGFILRGGDGGRGLSSSP PRPRAPGFLPQGGLGCPLWQEVGPGAPAWRCMR SGCAAFPRLQERPAPLPPRALAPGPAGRPATAGR RHLQSHPRAGSGA*CLGGLALAARRHPSHHSST CSLEKGR*VSRARSPASPWPPSRKRTALRSGAG AGRASSLRRSPAKCQHLRDLGADQREAKTRVLPR PGKPPQLQEGNQGADPKNWRRRKRRASRRSPRR RSSDPCVPPAPHWRSSFLLGLDSFAPLPPPPPL PQAHHHHRLWPPPPSSTCALTTTLHSTPAAAGLP WAEWGAVFPWPQFPAPPAHPRIHTCPPGQG*HP T*PHPAPAASPLPWWWGHCSLGFWFGGALSAPS LFPLASHSGAWEGSPGLKRGPSPISSWHALLHCP GSSRCGQWRGVLAPRIPPAKLSLSPRGAHFSSF PNFPSPRTRLDSPLRLQEGRRGESPTPSSLWPQ PPCPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT* GGLGLLP*PYFASATQPFPPPQMGHQ*QGAHPA RSQPPSCSSLPPKVLVPFFLCSQTTSGQLCCFL FNVPFFDIRHCCCYQRQMFILIASCSAHDPLPQD TLCGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQ SEQGGPNRP*RV*GPPPPPVLLGRGSHDLSQPG APSLVSYLQLLE*KKYPFLDW

Frame 2

WN*SL*LPVSILFSSGALFCGEGTEGGGFLPLP RARARQVSCPKAGLGAPSGRKWGRVHPRGGACG RGALLSRACRSGLRPSPRAPWPRVPPAGLQRPG ADTCRVTLGPGLEPDASGV*P*PLGGIPAITLPP AP*RREDE*VELEVQPALGLQAGKGRH*EAGPG QAAQAASEGAQRSANT*ETSGPTKGKQKQGCCQ DPENHHNSRKETKGQTQKTGEGGRGGHLAGVLGG GAVTHACRLLLTGGAASFWDWTALLRSHRPHPF PRPTITTASGRHPHLPPVPSPPHYTAHQPLQGS HGLSGEQFSPGLSSQLPPPTHAYTHALLDKANIP LSRTLHLLRPHSLGGGDIALWAFGLGAPSLLLH CSLWLPIVGPGRVPLALKGAQAPSHPGTPYSTA LAAAGVANGGGCWPPGFPQPNCLCHHVGLTFHPS PTSLVPVLGWTAPFGYRKAGGVSPLLPLHCGHS PLALRLGSEYILW*WRCSHLLSR*GPRALWPPP EVGWGCSPNPTLLPPLSHFPLLRWGTNNKELTLP APNPPPAPPCPPRFWFHFSSVHKLPLDSCVVFC SMFHSSTSVIAAATSAKCSSSLPPVLPTIPSPK ILFVGKRGWGMAGWVTDYPSPREGGALPLGCCSR VSKGAQIDHKGCRGHLLPLFCWGGVAMICPSLG LPLWFPICSYLNKKNILFWTG

Sequence HMGA1.vgAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCC AGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACC TAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCC AAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGC TTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCA CCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCC CCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGG GGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCC TTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGAT TCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTA CAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGA TGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCA GCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTT CCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCC AAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCG ACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCT CCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATC CTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRSIPAITLPPAP*RREDE*VELEVQP ALGLQAGKGRH*EAGPGQAAQAASEGAQRSANT *ETSGPTKGKQKQGCCQDPENHHNSRKETKGQT QKTGEGGRGGHLAGVLGGGAVTHACRLLLTGGAA SFWDWTALLRSHRPHPFPRPTITTASGRHPHLP PVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQL PPPTHAYTHALLDKANIPLSRTLHLLRPHSLGGG DIALWAFGLGAPSLLLHCSLWLPIVGPGRVPLA LKGAQAPSHPGTPYSTALAAAGVANGGGCWPPG FPQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFGY RKAGGVSPLLPLHCGHSPLALRLGSEYILW*WR CSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPL SHFPLLRWGTNNKELTLPAPNPPPAPPCPPRFWF HFSSVHKLPLDSCVVFCSMFHSSTSVIAAATSA KCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVT DYPSPREGGALPLGCCSRVSKGAQIDHKGCRGHL LPLFCWGGVAMICPSLGLPLWFPICSYLNKKNI LFWTG

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEASQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPKEPSEVPTP KRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRP KKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQ LPSGTGQLCSAPTAPTPSPGPPSPPPLAATPIFH LCPHHHTTQHTSRCRAPMG*VGSSFPLASVPSS PRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVV GTLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPWP *KGPKPHLILARPTPLPWQQQVWPMEGGAGPQD SPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSV TGRQEG*VPYSLFTVATAPLPSAWDLSTYCGDGD AVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRHS AISPSSDGAPITRSSPCPLPTPLLLLPAPQGSG SIFPLFTNYLWTVVLFFVQCSILRHPSLLLLPAP NVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG*P TTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGAT SSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKIS FSGLE

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKHPSHHSSTCSLEKGR*VSRARSPA SPWPPSRKRTALRSGAGAGRASSLRRSPAKCQH LRDLGADQREAKTRVLPRPGKPPQLQEGNQGADP KNWRRRKRRASRRSPRRRSSDPCVPPAPHWRSS FLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSS TCALTTTLHSTPAAAGLPWAEWGAVFPWPQFPAP PAHPRIHTCPPGQG*HPT*PHPAPAASPLPWWW GHCSLGFWFGGALSAPSLFPLASHSGAWEGSPG LKRGPSPISSWHALLHCPGSSRCGQWRGVLAPRI PPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLRL QEGRRGESPTPSSLWPQPPCPPPGI*VHIVVME MQSLIVQVRPKSPVAAT*GGLGLLP*PYFASATQ PFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVLV PFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQR QMFILIASCSAHDPLPQDTLCGEEGLGHGRLGDR LPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GPP PPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKY PFLDW

Sequence HMGA1.vdAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAG CCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACA CCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGAC CCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCA GCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTC CACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCT CCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGT GGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGG CCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGG ATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGT TACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGA GATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACT CAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGG TTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCG CCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGAC CGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCAC CTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATA TCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRPSQPSLFHLLLREGKMSESSSKSS QPLASKQEKDGTEKRGRGRPRKQPPKEPSEVPT PKRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGR PKKLEKEEEEGISQESSEEEQ*PMRAACSSLEEQ LPSGTGQLCSAPTAPTPSPGPPSPPPLAATPIF HLCPHHHTTQHTSRCRAPMG*VGSSFPLASVPS SPRPPTHTHMPSWTRLTSHLAAPCTCCVPTPLVV GTLLSGLLVWGRPLCSFTVPSGFP*WGLGGFPW P*KGPKPHLILARPTPLPWQQQVWPMEGGAGPQ DSPSQTVFVTTWGSLFILPQLP*SPY*VGQPPSV TGRQEG*VPYSLFTVATAPLPSAWDLSTYCGDG DAVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRH SAISPSSDGAPITRSSPCPLPTPLLLLPAPQGSG SIFPLFTNYLWTVVLFFVQCSILRHPSLLLLPA PNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG* PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGAT SSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKKI SFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGHPSHHSSTCSLEKGR*VSRARSP ASPWPPSRKRTALRSGAGAGRASSLRRSPAKCQH LRDLGADQREAKTRVLPRPGKPPQLQEGNQGAD PKNWRRRKRRASRRSPRRRSSDPCVPPAPHWRS SFLLGLDSFAPLPPPPPLPQAHHHHRLWPPPPSS TCALTTTLHSTPAAAGLPWAEWGAVFPWPQFPA PPAHPRIHTCPPGQG*HPT*PHPAPAASPLPWW WGHCSLGFWFGGALSAPSLFPLASHSGAWEGSPG LKRGPSPISSWHALLHCPGSSRCGQWRGVLAPR IPPAKLSLSPRGAHFSSFPNFPSPRTRLDSPLR LQEGRRGESPTPSSLWPQPPCPPPGI*VHIVVME MQSLIVQVRPKSPVAAT*GGLGLLP*PYFASAT QPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVL VPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQR QMFILIASCSAHDPLPQDTLCGEEGLGHGRLGD RLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GP PPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KKY PFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKAIPAITLPPAP*RREDE*VELEVQ PALGLQAGKGRH*EAGPGQAAQAASEGAQRSAN T*ETSGPTKGKQKQGCCQDPENHHNSRKETKGQT QKTGEGGRGGHLAGVLGGGAVTHACRLLLTGGA ASFWDWTALLRSHRPHPFPRPTITTASGRHPHL PPVPSPPHYTAHQPLQGSHGLSGEQFSPGLSSQL PPPTHAYTHALLDKANIPLSRTLHLLRPHSLGG GDIALWAFGLGAPSLLLHCSLWLPIVGPGRVPL ALKGAQAPSHPGTPYSTALAAAGVANGGGCWPPG FPQPNCLCHHVGLTFHPSPTSLVPVLGWTAPFG YRKAGGVSPLLPLHCGHSPLALRLGSEYILW*W RCSHLLSR*GPRALWPPPEVGWGCSPNPTLLPPL SHFPLLRWGTNNKELTLPAPNPPPAPPCPPRFW FHFSSVHKLPLDSCVVFCSMFHSSTSVIAAATS AKCSSSLPPVLPTIPSPKILFVGKRGWGMAGWVT DYPSPREGGALPLGCCSRVSKGAQIDHKGCRGH LLPLFCWGGVAMICPSLGLPLWFPICSYLNKKN ILFWTG

Sequence HMGA1.vbAug05

CAGGCTGGTCTCGAACTCCTGACCTCAAGCAGTCCTCCTGCTTTGGCATCCCAAAGTGCTGGGATTACAGCATCCCAGCCATCACTCTTCCACCTGCTCC TTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCC GCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGG AAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGC AGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGG CCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGC TGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAG CCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCC CTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAG CAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAAC TTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTT GCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGG GCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCC CAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATG TTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCT TTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAG CAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCT CTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

QAGLELLTSSSPPALASQSAGITASQPSLFHLL LREGKMSESSSKSSQPLASKQEKDGTEKRGRGRP RKQPPKEPSEVPTPKRPRGRPKGSKNKGAAKTR KTTTTPGRKPRGRPKKLEKEEEEGISQESSEEE Q*PMRAACSSLEEQLPSGTGQLCSAPTAPTPSPG PPSPPPLAATPIFHLCPHHHTTQHTSRCRAPMG *VGSSFPLASVPSSPRPPTHTHMPSWTRLTSHL AAPCTCCVPTPLVVGTLLSGLLVWGRPLCSFTVP SGFP*WGLGGFPWP*KGPKPHLILARPTPLPWQ QQVWPMEGGAGPQDSPSQTVFVTTWGSLFILPQ LP*SPY*VGQPPSVTGRQEG*VPYSLFTVATAPL PSAWDLSTYCGDGDAVTYCPGEAQEPCGRHLRW AGAAPLTLLCFRHSAISPSSDGAPITRSSPCPL PTPLLLLPAPQGSGSIFPLFTNYLWTVVLFFVQC SILRHPSLLLLPAPNVHPHCLLFCPRSPPPRYS LWGRGAGAWQAG*PTTPVPGKVGPCP*DAAAE* ARGPKSTIKGVGATSSPCSVGEG*P*FVPAWGSL SGFLFAVT*IKKISFSGLE

Frame 1

RLVSNS*PQAVLLLWHPKVLGLQHPSHHSSTCS LEKGR*VSRARSPASPWPPSRKRTALRSGAGAG RASSLRRSPAKCQHLRDLGADQREAKTRVLPRPG KPPQLQEGNQGADPKNWRRRKRRASRRSPRRRS SDPCVPPAPHWRSSFLLGLDSFAPLPPPPPLPQ AHHHHRLWPPPPSSTCALTTTLHSTPAAAGLPWA EWGAVFPWPQFPAPPAHPRIHTCPPGQG*HPT* PHPAPAASPLPWWWGHCSLGFWFGGALSAPSLF PLASHSGAWEGSPGLKRGPSPISSWHALLHCPGS SRCGQWRGVLAPRIPPAKLSLSPRGAHFSSFPN FPSPRTRLDSPLRLQEGRRGESPTPSSLWPQPP CPPPGI*VHIVVMEMQSLIVQVRPKSPVAAT*GG LGLLP*PYFASATQPFPPPQMGHQ*QGAHPARS QPPSCSSLPPKVLVPFFLCSQTTSGQLCCFLFN VPFFDIRHCCCYQRQMFILIASCSAHDPLPQDTL CGEEGLGHGRLGDRLPQSQGRWGPAPRMLQQSE QGGPNRP*RV*GPPPPPVLLGRGSHDLSQPGAP SLVSYLQLLE*KKYPFLDW

Frame 2

GWSRTPDLKQSSCFGIPKCWDYSIPAITLPPAP *RREDE*VELEVQPALGLQAGKGRH*EAGPGQA AQAASEGAQRSANT*ETSGPTKGKQKQGCCQDP ENHHNSRKETKGQTQKTGEGGRGGHLAGVLGGGA VTHACRLLLTGGAASFWDWTALLRSHRPHPFPR PTITTASGRHPHLPPVPSPPHYTAHQPLQGSHG LSGEQFSPGLSSQLPPPTHAYTHALLDKANIPLS RTLHLLRPHSLGGGDIALWAFGLGAPSLLLHCS LWLPIVGPGRVPLALKGAQAPSHPGTPYSTALA AAGVANGGGCWPPGFPQPNCLCHHVGLTFHPSPT SLVPVLGWTAPFGYRKAGGVSPLLPLHCGHSPL ALRLGSEYILW*WRCSHLLSR*GPRALWPPPEV GWGCSPNPTLLPPLSHFPLLRWGTNNKELTLPAP NPPPAPPCPPRFWFHFSSVHKLPLDSCVVFCSM FHSSTSVIAAATSAKCSSSLPPVLPTIPSPKIL FVGKRGWGMAGWVTDYPSPREGGALPLGCCSRVS KGAQIDHKGCRGHLLPLFCWGGVAMICPSLGLP LWFPICSYLNKKNILFWTG

Sequence HMGA1.pAug05

GAGCGCGAGTTGTGCACCCGGCGGAGCCCGGAGGAGCCCGGCGCACCTCGCGCGGCGGCCGCTCCCGCTGGAGCCGGAGCCCGAGCCCGAGCCCGGGCCC GGGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAA GGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAG GGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGG AGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTG CTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACA CAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACA TGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGT TTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTC ATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCA CCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCC CTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAG GCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGG CACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTA CCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTG TTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGG GGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGG GTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

ERELCTRRSPEEPGAPRAAAAPAGAGARARARA RASQPSLFHLLLREGKMSESSSKSSQPLASKQEK DGTEKRGRGRPRKQPPKEPSEVPTPKRPRGRPK GSKNKGAAKTRKTTTTPGRKPRGRPKKLEKEEE EGISQESSEEEQ*PMRAACSSLEEQLPSGTGQLC SAPTAPTPSPGPPSPPPLAATPIFHLCPHHHTT QHTSRCRAPMG*VGSSFPLASVPSSPRPPTHTH MPSWTRLTSHLAAPCTCCVPTPLVVGTLLSGLLV WGRPLCSFTVPSGFP*WGLGGFPWP*KGPKPHL ILARPTPLPWQQQVWPMEGGAGPQDSPSQTVFV TTWGSLFILPQLP*SPY*VGQPPSVTGRQEG*VP YSLFTVATAPLPSAWDLSTYCGDGDAVTYCPGE AQEPCGRHLRWAGAAPLTLLCFRHSAISPSSDG APITRSSPCPLPTPLLLLPAPQGSGSIFPLFTNY LWTVVLFFVQCSILRHPSLLLLPAPNVHPHCLL FCPRSPPPRYSLWGRGAGAWQAG*PTTPVPGKV GPCP*DAAAE*ARGPKSTIKGVGATSSPCSVGEG *P*FVPAWGSLSGFLFAVT*IKKISFSGLE

Frame 1

SASCAPGGARRSPAHLARRPLPLEPEPEPEPGP GHPSHHSSTCSLEKGR*VSRARSPASPWPPSRK RTALRSGAGAGRASSLRRSPAKCQHLRDLGADQR EAKTRVLPRPGKPPQLQEGNQGADPKNWRRRKR RASRRSPRRRSSDPCVPPAPHWRSSFLLGLDSF APLPPPPPLPQAHHHHRLWPPPPSSTCALTTTLH STPAAAGLPWAEWGAVFPWPQFPAPPAHPRIHT CPPGQG*HPT*PHPAPAASPLPWWWGHCSLGFW FGGALSAPSLFPLASHSGAWEGSPGLKRGPSPIS SWHALLHCPGSSRCGQWRGVLAPRIPPAKLSLS PRGAHFSSFPNFPSPRTRLDSPLRLQEGRRGES PTPSSLWPQPPCPPPGI*VHIVVMEMQSLIVQVR PKSPVAAT*GGLGLLP*PYFASATQPFPPPQMG HQ*QGAHPARSQPPSCSSLPPKVLVPFFLCSQT TSGQLCCFLFNVPFFDIRHCCCYQRQMFILIASC SAHDPLPQDTLCGEEGLGHGRLGDRLPQSQGRW GPAPRMLQQSEQGGPNRP*RV*GPPPPPVLLGR GSHDLSQPGAPSLVSYLQLLE*KKYPFLDW

Frame 2

ARVVHPAEPGGARRTSRGGRSRWSRSPSPSPGP GIPAITLPPAP*RREDE*VELEVQPALGLQAGK GRH*EAGPGQAAQAASEGAQRSANT*ETSGPTK GKQKQGCCQDPENHHNSRKETKGQTQKTGEGGRG GHLAGVLGGGAVTHACRLLLTGGAASFWDWTAL LRSHRPHPFPRPTITTASGRHPHLPPVPSPPHY TAHQPLQGSHGLSGEQFSPGLSSQLPPPTHAYTH ALLDKANIPLSRTLHLLRPHSLGGGDIALWAFG LGAPSLLLHCSLWLPIVGPGRVPLALKGAQAPS HPGTPYSTALAAAGVANGGGCWPPGFPQPNCLCH HVGLTFHPSPTSLVPVLGWTAPFGYRKAGGVSP LLPLHCGHSPLALRLGSEYILW*WRCSHLLSR* GPRALWPPPEVGWGCSPNPTLLPPLSHFPLLRWG TNNKELTLPAPNPPPAPPCPPRFWFHFSSVHKL PLDSCVVFCSMFHSSTSVIAAATSAKCSSSLPP VLPTIPSPKILFVGKRGWGMAGWVTDYPSPREGG ALPLGCCSRVSKGAQIDHKGCRGHLLPLFCWGG VAMICPSLGLPLWFPICSYLNKKNILFWTG

Sequence HMGA1.jAug05

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRK PRGRPKKLEKEEEEGISQESSEEEQ*PMRAACS SLEEQLPSGTGQLCSAPTAPLPSAWDLSTYCGD GDAVTYCPGEAQEPCGRHLRWAGAAPLTLLCFRH SAISPSSDGAPITRSSPCPLPTPLLLLPAPQGS GSIFPLFTNYLWTVVLFFVQCSILRHPSLLLLP APNVHPHCLLFCPRSPPPRYSLWGRGAGAWQAG* PTTPVPGKVGPCP*DAAAE*ARGPKSTIKGVGA TSSPCSVGEG*P*FVPAWGSLSGFLFAVT*IKK ISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP AKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPAP HWRSSFLLGLDSFAPLPQPPCPPPGI*VHIVVM EMQSLIVQVRPKSPVAAT*GGLGLLP*PYFASA TQPFPPPQMGHQ*QGAHPARSQPPSCSSLPPKVL VPFFLCSQTTSGQLCCFLFNVPFFDIRHCCCYQ RQMFILIASCSAHDPLPQDTLCGEEGLGHGRLG DRLPQSQGRWGPAPRMLQQSEQGGPNRP*RV*GP PPPPVLLGRGSHDLSQPGAPSLVSYLQLLE*KK YPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ RSANT*ETSGPTKGKQKQGCCQDPENHHNSRKE TKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL LTGGAASFWDWTALLRSHSPLALRLGSEYILW*W RCSHLLSR*GPRALWPPPEVGWGCSPNPTLLPP LSHFPLLRWGTNNKELTLPAPNPPPAPPCPPRF WFHFSSVHKLPLDSCVVFCSMFHSSTSVIAAATS AKCSSSLPPVLPTIPSPKILFVGKRGWGMAGWV TDYPSPREGGALPLGCCSRVSKGAQIDHKGCRG HLLPLFCWGGVAMICPSLGLPLWFPICSYLNKKN ILFWTG

Sequence HMGA1.iAug05

TGGCGCGGGCGGGCCCGTCCCCCCTGCCCCTGGGTCGCTCTTTTTAAGCTCCCCTGAGCCGGTGCTGCGCTCCTCTAATTGGGACTCCGAGCCGGGGCTA TTTCTGGCGCTGGCGCGGCTCCAAGAAGGCATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCC CGGCAACCCGGAGCGCGCACCGCAGGCCGGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCG AGCTCGAAGTCCAGCCAGCCCTTGGCCTCCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCA GCGAAGTGCCAACACCTAAGAGACCTCGGGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAA ACCAAGGGGCAGACCCAAAAAACTGGAGAAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCC TCACTGGAGGAGCAGCTTCCTTCTGGGACTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCG CCACCCCCATCTTCCACCTGTGCCCTCACCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGC CTCAGTTCCCAGCTCCCCCCGCCCACCCACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCC ACTCCCTTGGTGGTGGGGACATTGCTCTCTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGG GAGGGTTCCCCTGGCCTTAAAAGGGGCCCAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGG TGCTGGCCCCCAGGATTCCCCCAGCCAAACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGA CAGCCCCCTTCGGTTACAGGAAGGCAGGAGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACAT ATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACT TTGCTTCCGCCACTCAGCCATTTCCCCCTCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCC CCCCAAGGTTCTGGTTCCATTTTTCCTCTGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGC TGCTGCTACCAGCGCCAAATGTTCATCCTCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATG GCAGGCTGGGTGACCGACTACCCCAGTCCCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAG GGTGTAGGGGCCACCTCCTCCCCCTGTTCTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTT GAATAAAAAAAATATCCTTTTCTGGACTGGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

WRGRARPPCPWVALFKLP*AGAALL*LGLRAGA ISGAGAAPRRHPHLLPAAAAAEPGRSSAPSTAAP GNPERAPQAGGRARASQPSLFHLLLREGKMSES SSKSSQPLASKQEKDGTEKRGRGRPRKQPPKEP SEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRK PRGRPKKLEKEEEEGISQESSEEEQ*PMRAACS SLEEQLPSGTGQLCSAPTAPTPSPGPPSPPPLA ATPIFHLCPHHHTTQHTSRCRAPMG*VGSSFPLA SVPSSPRPPTHTHMPSWTRLTSHLAAPCTCCVP TPLVVGTLLSGLLVWGRPLCSFTVPSGFP*WGL GGFPWP*KGPKPHLILARPTPLPWQQQVWPMEGG AGPQDSPSQTVFVTTWGSLFILPQLP*SPY*VG QPPSVTGRQEG*VPYSLFTVATAPLPSAWDLST YCGDGDAVTYCPGEAQEPCGRHLRWAGAAPLTLL CFRHSAISPSSDGAPITRSSPCPLPTPLLLLPA PQGSGSIFPLFTNYLWTVVLFFVQCSILRHPSL LLLPAPNVHPHCLLFCPRSPPPRYSLWGRGAGAW QAG*PTTPVPGKVGPCP*DAAAE*ARGPKSTIK GVGATSSPCSVGEG*P*FVPAWGSLSGFLFAVT *IKKISFSGLE

Frame 1

GAGGPVPPAPGSLFLSSPEPVLRSSNWDSEPGL FLALARLQEGIRICYQRRPRRSQAGPQRPAPPL PATRSAHRRPAAELAHPSHHSSTCSLEKGR*VSR ARSPASPWPPSRKRTALRSGAGAGRASSLRRSP AKCQHLRDLGADQREAKTRVLPRPGKPPQLQEG NQGADPKNWRRRKRRASRRSPRRRSSDPCVPPAP HWRSSFLLGLDSFAPLPPPPPLPQAHHHHRLWP PPPSSTCALTTTLHSTPAAAGLPWAEWGAVFPW PQFPAPPAHPRIHTCPPGQG*HPT*PHPAPAASP LPWWWGHCSLGFWFGGALSAPSLFPLASHSGAW EGSPGLKRGPSPISSWHALLHCPGSSRCGQWRG VLAPRIPPAKLSLSPRGAHFSSFPNFPSPRTRLD SPLRLQEGRRGESPTPSSLWPQPPCPPPGI*VH IVVMEMQSLIVQVRPKSPVAAT*GGLGLLP*PY FASATQPFPPPQMGHQ*QGAHPARSQPPSCSSLP PKVLVPFFLCSQTTSGQLCCFLFNVPFFDIRHC CCYQRQMFILIASCSAHDPLPQDTLCGEEGLGH GRLGDRLPQSQGRWGPAPRMLQQSEQGGPNRP*R V*GPPPPPVLLGRGSHDLSQPGAPSLVSYLQLL E*KKYPFLDW

Frame 2

ARAGPSPLPLGRSF*APLSRCCAPLIGTPSRGY FWRWRGSKKASAFATSGGRGGARPVLSAQHRRS RQPGARTAGRRPSSRIPAITLPPAP*RREDE*V ELEVQPALGLQAGKGRH*EAGPGQAAQAASEGAQ RSANT*ETSGPTKGKQKQGCCQDPENHHNSRKE TKGQTQKTGEGGRGGHLAGVLGGGAVTHACRLL LTGGAASFWDWTALLRSHRPHPFPRPTITTASGR HPHLPPVPSPPHYTAHQPLQGSHGLSGEQFSPG LSSQLPPPTHAYTHALLDKANIPLSRTLHLLRP HSLGGGDIALWAFGLGAPSLLLHCSLWLPIVGPG RVPLALKGAQAPSHPGTPYSTALAAAGVANGGG CWPPGFPQPNCLCHHVGLTFHPSPTSLVPVLGW TAPFGYRKAGGVSPLLPLHCGHSPLALRLGSEYI LW*WRCSHLLSR*GPRALWPPPEVGWGCSPNPT LLPPLSHFPLLRWGTNNKELTLPAPNPPPAPPC PPRFWFHFSSVHKLPLDSCVVFCSMFHSSTSVIA AATSAKCSSSLPPVLPTIPSPKILFVGKRGWGM AGWVTDYPSPREGGALPLGCCSRVSKGAQIDHK GCRGHLLPLFCWGGVAMICPSLGLPLWFPICSYL NKKNILFWTG

Sequence HMGA1.hAug05

GCTCCTCTAATTGGGACTCCGAGCCGGGGCTATTTCTGGCGCTGGCGCGGCTCCAAGAAGGCGTGAGTTCGCGGCCGCTCCGGTGGCTTCTTTTTTTTAT ATCTATAATTTAATTAAATTATTTATTTATTGAGGCCGCGCACGGGCCGTGCCCAGCTTCCTGCCCCTCGCCATCCTTCGGGGGAGGGGGAATATTTTTG TCCCCCCGCCTGGCTGTGACACATAAATACCCCGCGGGGGCCTGGGCGGCGAGCACGCGGCGGCGGCGGTCTCTGAGCGCCTCTGCTCTCTCCCGGTTTC AGATCCGCATTTGCTACCAGCGGCGGCCGCGGCGGAGCCAGGCCGGTCCTCAGCGCCCAGCACCGCCGCTCCCGGCAACCCGGAGCGCGCACCGCAGGCC GGCGGCCGAGCTCGCGCATCCCAGCCATCACTCTTCCACCTGCTCCTTAGAGAAGGGAAGATGAGTGAGTCGAGCTCGAAGTCCAGCCAGCCCTTGGCCT CCAAGCAGGAAAAGGACGGCACTGAGAAGCGGGGCCGGGGCAGGCCGCGCAAGCAGCCTCCGAAGGAGCCCAGCGAAGTGCCAACACCTAAGAGACCTCG GGGCCGACCAAAGGGAAGCAAAAACAAGGGTGCTGCCAAGACCCGGAAAACCACCACAACTCCAGGAAGGAAACCAAGGGGCAGACCCAAAAAACTGGAG AAGGAGGAAGAGGAGGGCATCTCGCAGGAGTCCTCGGAGGAGGAGCAGTGACCCATGCGTGCCGCCTGCTCCTCACTGGAGGAGCAGCTTCCTTCTGGGA CTGGACAGCTTTGCTCCGCTCCCACCGCCCCCACCCCTTCCCCAGGCCCACCATCACCACCGCCTCTGGCCGCCACCCCCATCTTCCACCTGTGCCCTCA CCACCACACTACACAGCACACCAGCCGCTGCAGGGCTCCCATGGGCTGAGTGGGGAGCAGTTTTCCCCTGGCCTCAGTTCCCAGCTCCCCCCGCCCACCC ACGCATACACACATGCCCTCCTGGACAAGGCTAACATCCCACTTAGCCGCACCCTGCACCTGCTGCGTCCCCACTCCCTTGGTGGTGGGGACATTGCTCT CTGGGCTTTTGGTTTGGGGGCGCCCTCTCTGCTCCTTCACTGTTCCCTCTGGCTTCCCATAGTGGGGCCTGGGAGGGTTCCCCTGGCCTTAAAAGGGGCC CAAGCCCCATCTCATCCTGGCACGCCCTACTCCACTGCCCTGGCAGCAGCAGGTGTGGCCAATGGAGGGGGGTGCTGGCCCCCAGGATTCCCCCAGCCAA ACTGTCTTTGTCACCACGTGGGGCTCACTTTTCATCCTTCCCCAACTTCCCTAGTCCCCGTACTAGGTTGGACAGCCCCCTTCGGTTACAGGAAGGCAGG AGGGGTGAGTCCCCTACTCCCTCTTCACTGTGGCCACAGCCCCCTTGCCCTCCGCCTGGGATCTGAGTACATATTGTGGTGATGGAGATGCAGTCACTTA TTGTCCAGGTGAGGCCCAAGAGCCCTGTGGCCGCCACCTGAGGTGGGCTGGGGCTGCTCCCCTAACCCTACTTTGCTTCCGCCACTCAGCCATTTCCCCC TCCTCAGATGGGGCACCAATAACAAGGAGCTCACCCTGCCCGCTCCCAACCCCCCTCCTGCTCCTCCCTGCCCCCCAAGGTTCTGGTTCCATTTTTCCTC TGTTCACAAACTACCTCTGGACAGTTGTGTTGTTTTTTGTTCAATGTTCCATTCTTCGACATCCGTCATTGCTGCTGCTACCAGCGCCAAATGTTCATCC TCATTGCCTCCTGTTCTGCCCACGATCCCCTCCCCCAAGATACTCTTTGTGGGGAAGAGGGGCTGGGGCATGGCAGGCTGGGTGACCGACTACCCCAGTC CCAGGGAAGGTGGGGCCCTGCCCCTAGGATGCTGCAGCAGAGTGAGCAAGGGGGCCCAAATCGACCATAAAGGGTGTAGGGGCCACCTCCTCCCCCTGTT CTGTTGGGGAGGGGTAGCCATGATTTGTCCCAGCCTGGGGCTCCCTCTCTGGTTTCCTATTTGCAGTTACTTGAATAAAAAAAATATCCTTTTCTGGACT GGAG

HMGA1.au.0.f (forward primer)
HMGA1.u.r.18 (reverse primer)
Alternative acceptor

Frame 0

APLIGTPSRGYFWRWRGSKKA*VRGRSGGFFFL YL*FN*IIYLLRPRTGRAQLPAPRHPSGEGEYFC PPAWL*HINTPRGPGRRARGGGGL*APLLSPGF RSAFATSGGRGGARPVLSAQHRRSRQPGARTAG RRPSSRIPAITLPPAP*RREDE*VELEVQPALGL QAGKGRH*EAGPGQAAQAASEGAQRSANT*ETS GPTKGKQKQGCCQDPENHHNSRKETKGQTQKTG EGGRGGHLAGVLGGGAVTHACRLLLTGGAASFWD WTALLRSHRPHPFPRPTITTASGRHPHLPPVPS PPHYTAHQPLQGSHGLSGEQFSPGLSSQLPPPT HAYTHALLDKANIPLSRTLHLLRPHSLGGGDIAL WAFGLGAPSLLLHCSLWLPIVGPGRVPLALKGA QAPSHPGTPYSTALAAAGVANGGGCWPPGFPQP NCLCHHVGLTFHPSPTSLVPVLGWTAPFGYRKAG GVSPLLPLHCGHSPLALRLGSEYILW*WRCSHL LSR*GPRALWPPPEVGWGCSPNPTLLPPLSHFP LLRWGTNNKELTLPAPNPPPAPPCPPRFWFHFSS VHKLPLDSCVVFCSMFHSSTSVIAAATSAKCSS SLPPVLPTIPSPKILFVGKRGWGMAGWVTDYPS PREGGALPLGCCSRVSKGAQIDHKGCRGHLLPLF CWGGVAMICPSLGLPLWFPICSYLNKKNILFWT G

Frame 1

LL*LGLRAGAISGAGAAPRRREFAAAPVASFFY IYNLIKLFIY*GRARAVPSFLPLAILRGRGNIF VPPPGCDT*IPRGGLGGEHAAAAVSERLCSLPVS DPHLLPAAAAAEPGRSSAPSTAAPGNPERAPQA GGRARASQPSLFHLLLREGKMSESSSKSSQPLA SKQEKDGTEKRGRGRPRKQPPKEPSEVPTPKRPR GRPKGSKNKGAAKTRKTTTTPGRKPRGRPKKLE KEEEEGISQESSEEEQ*PMRAACSSLEEQLPSG TGQLCSAPTAPTPSPGPPSPPPLAATPIFHLCPH HHTTQHTSRCRAPMG*VGSSFPLASVPSSPRPP THTHMPSWTRLTSHLAAPCTCCVPTPLVVGTLL SGLLVWGRPLCSFTVPSGFP*WGLGGFPWP*KGP KPHLILARPTPLPWQQQVWPMEGGAGPQDSPSQ TVFVTTWGSLFILPQLP*SPY*VGQPPSVTGRQ EG*VPYSLFTVATAPLPSAWDLSTYCGDGDAVTY CPGEAQEPCGRHLRWAGAAPLTLLCFRHSAISP SSDGAPITRSSPCPLPTPLLLLPAPQGSGSIFP LFTNYLWTVVLFFVQCSILRHPSLLLLPAPNVHP HCLLFCPRSPPPRYSLWGRGAGAWQAG*PTTPV PGKVGPCP*DAAAE*ARGPKSTIKGVGATSSPC SVGEG*P*FVPAWGSLSGFLFAVT*IKKISFSGL E

Frame 2

SSNWDSEPGLFLALARLQEGVSSRPLRWLLFFI SII*LNYLFIEAAHGPCPASCPSPSFGGGGIFL SPRLAVTHKYPAGAWAASTRRRRSLSASALSRF QIRICYQRRPRRSQAGPQRPAPPLPATRSAHRRP AAELAHPSHHSSTCSLEKGR*VSRARSPASPWP PSRKRTALRSGAGAGRASSLRRSPAKCQHLRDL GADQREAKTRVLPRPGKPPQLQEGNQGADPKNWR RRKRRASRRSPRRRSSDPCVPPAPHWRSSFLLG LDSFAPLPPPPPLPQAHHHHRLWPPPPSSTCAL TTTLHSTPAAAGLPWAEWGAVFPWPQFPAPPAHP RIHTCPPGQG*HPT*PHPAPAASPLPWWWGHCS LGFWFGGALSAPSLFPLASHSGAWEGSPGLKRG PSPISSWHALLHCPGSSRCGQWRGVLAPRIPPAK LSLSPRGAHFSSFPNFPSPRTRLDSPLRLQEGR RGESPTPSSLWPQPPCPPPGI*VHIVVMEMQSL IVQVRPKSPVAAT*GGLGLLP*PYFASATQPFPP PQMGHQ*QGAHPARSQPPSCSSLPPKVLVPFFL CSQTTSGQLCCFLFNVPFFDIRHCCCYQRQMFI LIASCSAHDPLPQDTLCGEEGLGHGRLGDRLPQS QGRWGPAPRMLQQSEQGGPNRP*RV*GPPPPPV LLGRGSHDLSQPGAPSLVSYLQLLE*KKYPFLD W