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Sequences

Sequence TUBA1.hAug05

ATAAGGGCGGTGCGGCACTGCAGCTAGCGCAGTTCTCACTGAGACCTGTCACCCCGACTCAACGTGAGACGCACCGCCCGGACTCACCATGCGTGAATGC ATCTCAGTCCACGTGGGGCAGGCAGGTGTCCAGATGGGCAATGCCTGCTGGGAGCTCTATTGCTTGGAACATGGGATTCAGCCTGATGGGCAGATGCCCA GTGACAAGACCATTGGTGGAGGGGACGACTCCTTCACCACCTTCTTCTGTGAAACTGGTGCTGGAAAACACGTACCCCGGGCAGTTTTTGTGGATCTGGA GCCTACGGTCATTGATGAGATCCGAAATGGCCCATACCGACAGCTCTTCCACCCAGAGCAGCTCATCACTGGGAAAGAGGATGCTGCCAACAACTATGCC CGTGGTCACTATACCATTGGCAAGGAGATCATTGACCCAGTGCTGGATCGGATCCGCAATCTGACCAGTGCACAGGACTTCAGGGCTTCCTGGTGTTCCA CAGCTTTGGTGGGGGCACTGGCTCTGGCTTCACCTCACTCCTGATGGAGCGGCTCTCTGTTGACTATGGCAAGAAATCCAAGCTGGAATTCTCCATCTAC CCAGCCCCCCAGGTGTCTACAGCCGTGGTCGAGCCCTACAACTCTATCCTGACCACCCACACCACCCTGGAGCACTCAGACTGTGCCTTCATGGTGGACA ACGAAGCAATCTATGACATCTGCCGCCGCAACCTAGACATCGAGCGCCCAACCTACACCAACCTCAATCGCCTCATTAGCCAAATTGTCTCCTCCATCAC AGCTTCTCTGCGCTTTGACGGGGCCCTCAATGTGGACCTGACAGAGTTCCAGACCAACCTGGTGCCCTACCCTCGCATCCACTTCCCCCTGGCCACCTAT GCACCAGTCATCTCTGCAGAAAAGGCATACCACGAGCAGCTGTCGGTGGCAGAGATCACCAATGCCTGCTTTGAGCCTGCCAACCAGATGGTAAAGTGTG ATCCCCGGCACGGCAAGTACATGGCCTGCTGCCTGCTGTACCGTGGAGATGTGGTGCCCAAGGATGTCAACGCTGCCATTGCCGCCATCAAGACCAAGCG CAGCATTCAGTTTGTGGACTGGTGCCCCACAGGCTTCAAGGTTGGTATCAACTACCAGCCTCCCACTGTGGTGCCTGGGGGTGACCTGGCCAAGGTGCAG CGTGCCGTGTGCATGCTGAGCAACACGACCGCCATCGCCGAGGCCTGGGCCCGCCTGGACCACAAGTTCGACCTGATGTATGCCAAGAGGGCGTTTGTGC ACTGGTATGTGGGTGAGGGCATGGAGGAGGGTGAGTTCTCCGAGGCCCGTGAGGATATGGCTGCCCTGGAGAAGGATTATGAGGAGGTGGGCATCGACTC CTATGAGGACGAGGATGAGGGAGAAGAATAAAGCAGCTGCCTGGAGCCTATTCACTATGTTTATTGCAAAATCCTTTCGAAATAAACAGTTTCCTTGCAC GGTTTCTTGTTCCCTCTGAGTGCTTGAGCTTCTGCTGCCTTCCCCTTCATGCTGCTGCTGCTCTGTTTGCTGTTCATGACCCTTATCCCTTCCATCGCTG AAGGTGAGACCTGCAAAGGGGTTCTTGCCAGGCCCAGGAAGCATAACCCCAGGGACTTTAAATTGAAGTCCTAGGGTTACTCAGTATAGGGAGGAAATGA GGACCAAAATCCAGGCACAAACTGTTGAAGGATGAGTCTTCAGCTCCTTTCCAAGTAGGAGTATAATCCAGTGGTTTCTGTCTTTGAAAGTTAGACTGCT ACCCTGTTCTTCCTGCTGCCTCTACATTTACCAACTCTGGAAGCAAGGCTTGGCCCAGGCTCCTCTGTGCATTATGAGGGTTTGGGAGTGGGGCTGGGCT GGCCCAGAGTTCCTGGGCATGGAGCCACGTCATAAGCTTGGGGGATGGGAGGGAACACAGGCGATCTCCTAGAGTTGTTTGTGCCACTGCACCTCTGAGC AGCTTTGCATAGCTTCCTGTCTTGCTGCTTTGCTGGAGCTGGGGCTGTGCCATTAAAAGTCAGGTCATCTCCCCTCTGTTTGTGATTTCTTTCTGGCCTC AGCCTCTGTGCTAGCTAGTGGGAGACTCCTGCTAGGGCCCTGGGGAAAGCTTCAACTTTGCTAACAGGAGCCCCAGGCCCCATGCTGATGTCATACTGTG GGGTAGACATGGTTCGGGGGAGGAGGCTTGGATCACAAAAGAGCAGGAGATGCCAACCCTGCCCTTGCCTTTCCTCCCTGGTGATTCAGGGAACAGAGCC CTTCCCTAGGGAACAGGTTTGAAGCCCAGCTAAGACAGGGCC

TUBA1.F5 (forward primer)
TUBA1.R5 (reverse primer)
Intron retention

Frame 0

IRAVRHCS*RSSH*DLSPRLNVRRTARTHHA*M HLSPRGAGRCPDGQCLLGALLLGTWDSA*WADAQ *QDHWWRGRLLHHLLL*NWCWKTRTPGSFCGSG AYGH**DPKWPIPTALPPRAAHHWERGCCQQLC PWSLYHWQGDH*PSAGSDPQSDQCTGLQGFLVFH SFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIY PAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVD NEAIYDICRRNLDIERPTYTNLNRLISQIVSSIT ASLRFDGALNVDLTEFQTNLVPYPRIHFPLATY APVISAEKAYHEQLSVAEITNACFEPANQMVKC DPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKR SIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQ RAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFV HWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDS YEDEDEGEE*SSCLEPIHYVYCKILSK*TVSLH GFLFPLSA*ASAAFPFMLLLLCLLFMTLIPSIA EGETCKGVLARPRKHNPRDFKLKS*GYSV*GGNE DQNPGTNC*RMSLQLLSK*EYNPVVSVFES*TA TLFFLLPLHLPTLEARLGPGSSVHYEGLGVGLG WPRVPGHGATS*AWGMGGNTGDLLELFVPLHL*A ALHSFLSCCFAGAGAVPLKVRSSPLCL*FLSGL SLCAS*WETPARALGKASTLLTGAPGPMLMSYC GVDMVRGRRLGSQKSRRCQPCPCLSSLVIQGTEP FPREQV*SPAKTG

Frame 1

*GRCGTAASAVLTETCHPDST*DAPPGLTMREC ISVHVGQAGVQMGNACWELYCLEHGIQPDGQMP SDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLE PTVIDEIRNGPYRQLFHPEQLITGKEDAANNYA RGHYTIGKEIIDPVLDRIRNLTSAQDFRASWCS TALVGALALASPHS*WSGSLLTMARNPSWNSPST QPPRCLQPWSSPTTLS*PPTPPWSTQTVPSWWT TKQSMTSAAAT*TSSAQPTPTSIASLAKLSPPS QLLCALTGPSMWT*QSSRPTWCPTLASTSPWPPM HQSSLQKRHTTSSCRWQRSPMPALSLPTRW*SV IPGTASTWPAACCTVEMWCPRMSTLPLPPSRPS AAFSLWTGAPQASRLVSTTSLPLWCLGVTWPRCS VPCAC*ATRPPSPRPGPAWTTSST*CMPRGRLC TGMWVRAWRRVSSPRPVRIWLPWRRIMRRWAST PMRTRMREKNKAAAWSLFTMFIAKSFRNKQFPCT VSCSL*VLELLLPSPSCCCCSVCCS*PLSLPSL KVRPAKGFLPGPGSITPGTLN*SPRVTQYREEM RTKIQAQTVEG*VFSSFPSRSIIQWFLSLKVRLL PCSSCCLYIYQLWKQGLAQAPLCIMRVWEWGWA GPEFLGMEPRHKLGGWEGTQAIS*SCLCHCTSE QLCIASCLAALLELGLCH*KSGHLPSVCDFFLAS ASVLASGRLLLGPWGKLQLC*QEPQAPC*CHTV G*TWFGGGGLDHKRAGDANPALAFPPW*FREQS PSLGNRFEAQLRQG

Frame 2

KGGAALQLAQFSLRPVTPTQRETHRPDSPCVNA SQSTWGRQVSRWAMPAGSSIAWNMGFSLMGRCP VTRPLVEGTTPSPPSSVKLVLENTYPGQFLWIW SLRSLMRSEMAHTDSSSTQSSSSLGKRMLPTTMP VVTIPLARRSLTQCWIGSAI*PVHRTSGLPGVP QLWWGHWLWLHLTPDGAALC*LWQEIQAGILHL PSPPGVYSRGRALQLYPDHPHHPGALRLCLHGGQ RSNL*HLPPQPRHRAPNLHQPQSPH*PNCLLHH SFSAL*RGPQCGPDRVPDQPGALPSHPLPPGHL CTSHLCRKGIPRAAVGGRDHQCLL*ACQPDGKV* SPARQVHGLLPAVPWRCGAQGCQRCHCRHQDQA QHSVCGLVPHRLQGWYQLPASHCGAWG*PGQGA ACRVHAEQHDRHRRGLGPPGPQVRPDVCQEGVCA LVCG*GHGGG*VLRGP*GYGCPGEGL*GGGHRL L*GRG*GRRIKQLPGAYSLCLLQNPFEINSFLA RFLVPSECLSFCCLPLHAAAALFAVHDPYPFHR* R*DLQRGSCQAQEA*PQGL*IEVLGLLSIGRK* GPKSRHKLLKDESSAPFQVGV*SSGFCL*KLDC YPVLPAASTFTNSGSKAWPRLLCAL*GFGSGAGL AQSSWAWSHVISLGDGREHRRSPRVVCATAPLS SFA*LPVLLLCWSWGCAIKSQVISPLFVISFWP QPLC*LVGDSC*GPGESFNFANRSPRPHADVILW GRHGSGEEAWITKEQEMPTLPLPFLPGDSGNRA LP*GTGLKPS*DRA

Sequence TUBA1.aAug05

ATAAGGGCGGTGCGGCACTGCAGCTAGCGCAGTTCTCACTGAGACCTGTCACCCCGACTCAACGTGAGACGCACCGCCCGGACTCACCATGCGTGAATGC ATCTCAGTCCACGTGGGGCAGGCAGGTGTCCAGATGGGCAATGCCTGCTGGGAGCTCTATTGCTTGGAACATGGGATTCAGCCTGATGGGCAGATGCCCA GTGACAAGACCATTGGTGGAGGGGACGACTCCTTCACCACCTTCTTCTGTGAAACTGGTGCTGGAAAACACGTACCCCGGGCAGTTTTTGTGGATCTGGA GCCTACGGTCATTGATGAGATCCGAAATGGCCCATACCGACAGCTCTTCCACCCAGAGCAGCTCATCACTGGGAAAGAGGATGCTGCCAACAACTATGCC CGTGGTCACTATACCATTGGCAAGGAGATCATTGACCCAGTGCTGGATCGGATCCGCAAGCTGTCTGACCAGTGCACAGGACTTCAGGGCTTCCTGGTGT TCCACAGCTTTGGTGGGGGCACTGGCTCTGGCTTCACCTCACTCCTGATGGAGCGGCTCTCTGTTGACTATGGCAAGAAATCCAAGCTGGAATTCTCCAT CTACCCAGCCCCCCAGGTGTCTACAGCCGTGGTCGAGCCCTACAACTCTATCCTGACCACCCACACCACCCTGGAGCACTCAGACTGTGCCTTCATGGTG GACAACGAAGCAATCTATGACATCTGCCGCCGCAACCTAGACATCGAGCGCCCAACCTACACCAACCTCAATCGCCTCATTAGCCAAATTGTCTCCTCCA TCACAGCTTCTCTGCGCTTTGACGGGGCCCTCAATGTGGACCTGACAGAGTTCCAGACCAACCTGGTGCCCTACCCTCGCATCCACTTCCCCCTGGCCAC CTATGCACCAGTCATCTCTGCAGAAAAGGCATACCACGAGCAGCTGTCGGTGGCAGAGATCACCAATGCCTGCTTTGAGCCTGCCAACCAGATGGTAAAG TGTGATCCCCGGCACGGCAAGTACATGGCCTGCTGCCTGCTGTACCGTGGAGATGTGGTGCCCAAGGATGTCAACGCTGCCATTGCCGCCATCAAGACCA AGCGCAGCATTCAGTTTGTGGACTGGTGCCCCACAGGCTTCAAGGTTGGTATCAACTACCAGCCTCCCACTGTGGTGCCTGGGGGTGACCTGGCCAAGGT GCAGCGTGCCGTGTGCATGCTGAGCAACACGACCGCCATCGCCGAGGCCTGGGCCCGCCTGGACCACAAGTTCGACCTGATGTATGCCAAGAGGGCGTTT GTGCACTGGTATGTGGGTGAGGGCATGGAGGAGGGTGAGTTCTCCGAGGCCCGTGAGGATATGGCTGCCCTGGAGAAGGATTATGAGGAGGTGGGCATCG ACTCCTATGAGGACGAGGATGAGGGAGAAGAATAAAGCAGCTGCCTGGAGCCTATTCACTATGTTTATTGCAAAATCCTTTCGAAATAAACAGTTTCCTT GCACGGTTTCTTGTTCCCTCTGAGTGCTTGAGCTTCTGCTGCCTTCCCCTTCATGCTGCTGCTGCTCTGTTTGCTGTTCATGACCCTTATCCCTTCCATC GCTGAAGGTGAGACCTGCAAAGGGGTTCTTGCCAGGCCCAGGAAGCATAACCCCAGGGACTTTAAATTGAAGTCCTAGGGTTACTCAGTATAGGGAGGAA ATGAGGACCAAAATCCAGGCACAAACTGTTGAAGGATGAGTCTTCAGCTCCTTTCCAAGTAGGAGTATAATCCAGTGGTTTCTGTCTTTGAAAGTTAGAC TGCTACCCTGTTCTTCCTGCTGCCTCTACATTTACCAACTCTGGAAGCAAGGCTTGGCCCAGGCTCCTCTGTGCATTATGAGGGTTTGGGAGTGGGGCTG GGCTGGCCCAGAGTTCCTGGGCATGGAGCCACGTCATAAGCTTGGGGGATGGGAGGGAACACAGGCGATCTCCTAGAGTTGTTTGTGCCACTGCACCTCT GAGCAGCTTTGCATAGCTTCCTGTCTTGCTGCTTTGCTGGAGCTGGGGCTGTGCCATTAAAAGTCAGGTCATCTCCCCTCTGTTTGTGATTTCTTTCTGG CCTCAGCCTCTGTGCTAGCTAGTGGGAGACTCCTGCTAGGGCCCTGGGGAAAGCTTCAACTTTGCTAACAGGAGCCCCAGGCCCCATGCTGATGTCATAC TGTGGGGTAGACATGGTTCGGGGGAGGAGGCTTGGATCACAAAAGAGCAGGAGATGCCAACCCTGCCCTTGCCTTTCCTCCCTGGTGATTCAGGGAACAG AGCCCTTCCCTAGGGAACAGGTTTGAAGCCCAGCTAAGACAGGGCC

TUBA1.F5 (forward primer)
TUBA1.R5 (reverse primer)
Intron retention

Frame 0

IRAVRHCS*RSSH*DLSPRLNVRRTARTHHA*M HLSPRGAGRCPDGQCLLGALLLGTWDSA*WADAQ *QDHWWRGRLLHHLLL*NWCWKTRTPGSFCGSG AYGH**DPKWPIPTALPPRAAHHWERGCCQQLC PWSLYHWQGDH*PSAGSDPQAV*PVHRTSGLPGV PQLWWGHWLWLHLTPDGAALC*LWQEIQAGILH LPSPPGVYSRGRALQLYPDHPHHPGALRLCLHG GQRSNL*HLPPQPRHRAPNLHQPQSPH*PNCLLH HSFSAL*RGPQCGPDRVPDQPGALPSHPLPPGH LCTSHLCRKGIPRAAVGGRDHQCLL*ACQPDGK V*SPARQVHGLLPAVPWRCGAQGCQRCHCRHQDQ AQHSVCGLVPHRLQGWYQLPASHCGAWG*PGQG AACRVHAEQHDRHRRGLGPPGPQVRPDVCQEGV CALVCG*GHGGG*VLRGP*GYGCPGEGL*GGGHR LL*GRG*GRRIKQLPGAYSLCLLQNPFEINSFL ARFLVPSECLSFCCLPLHAAAALFAVHDPYPFH R*R*DLQRGSCQAQEA*PQGL*IEVLGLLSIGRK *GPKSRHKLLKDESSAPFQVGV*SSGFCL*KLD CYPVLPAASTFTNSGSKAWPRLLCAL*GFGSGA GLAQSSWAWSHVISLGDGREHRRSPRVVCATAPL SSFA*LPVLLLCWSWGCAIKSQVISPLFVISFW PQPLC*LVGDSC*GPGESFNFANRSPRPHADVI LWGRHGSGEEAWITKEQEMPTLPLPFLPGDSGNR ALP*GTGLKPS*DRA

Frame 1

*GRCGTAASAVLTETCHPDST*DAPPGLTMREC ISVHVGQAGVQMGNACWELYCLEHGIQPDGQMP SDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLE PTVIDEIRNGPYRQLFHPEQLITGKEDAANNYA RGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLV FHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSI YPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMV DNEAIYDICRRNLDIERPTYTNLNRLISQIVSS ITASLRFDGALNVDLTEFQTNLVPYPRIHFPLAT YAPVISAEKAYHEQLSVAEITNACFEPANQMVK CDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKT KRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKV QRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAF VHWYVGEGMEEGEFSEAREDMAALEKDYEEVGI DSYEDEDEGEE*SSCLEPIHYVYCKILSK*TVSL HGFLFPLSA*ASAAFPFMLLLLCLLFMTLIPSI AEGETCKGVLARPRKHNPRDFKLKS*GYSV*GG NEDQNPGTNC*RMSLQLLSK*EYNPVVSVFES*T ATLFFLLPLHLPTLEARLGPGSSVHYEGLGVGL GWPRVPGHGATS*AWGMGGNTGDLLELFVPLHL *AALHSFLSCCFAGAGAVPLKVRSSPLCL*FLSG LSLCAS*WETPARALGKASTLLTGAPGPMLMSY CGVDMVRGRRLGSQKSRRCQPCPCLSSLVIQGT EPFPREQV*SPAKTG

Frame 2

KGGAALQLAQFSLRPVTPTQRETHRPDSPCVNA SQSTWGRQVSRWAMPAGSSIAWNMGFSLMGRCP VTRPLVEGTTPSPPSSVKLVLENTYPGQFLWIW SLRSLMRSEMAHTDSSSTQSSSSLGKRMLPTTMP VVTIPLARRSLTQCWIGSASCLTSAQDFRASWC STALVGALALASPHS*WSGSLLTMARNPSWNSP STQPPRCLQPWSSPTTLS*PPTPPWSTQTVPSWW TTKQSMTSAAAT*TSSAQPTPTSIASLAKLSPP SQLLCALTGPSMWT*QSSRPTWCPTLASTSPWP PMHQSSLQKRHTTSSCRWQRSPMPALSLPTRW*S VIPGTASTWPAACCTVEMWCPRMSTLPLPPSRP SAAFSLWTGAPQASRLVSTTSLPLWCLGVTWPR CSVPCAC*ATRPPSPRPGPAWTTSST*CMPRGRL CTGMWVRAWRRVSSPRPVRIWLPWRRIMRRWAS TPMRTRMREKNKAAAWSLFTMFIAKSFRNKQFP CTVSCSL*VLELLLPSPSCCCCSVCCS*PLSLPS LKVRPAKGFLPGPGSITPGTLN*SPRVTQYREE MRTKIQAQTVEG*VFSSFPSRSIIQWFLSLKVR LLPCSSCCLYIYQLWKQGLAQAPLCIMRVWEWGW AGPEFLGMEPRHKLGGWEGTQAIS*SCLCHCTS EQLCIASCLAALLELGLCH*KSGHLPSVCDFFL ASASVLASGRLLLGPWGKLQLC*QEPQAPC*CHT VG*TWFGGGGLDHKRAGDANPALAFPPW*FREQ SPSLGNRFEAQLRQG

Sequence TUBA1.jAug05

TUBA1.F5 (forward primer)
TUBA1.R5 (reverse primer)
Intron retention

Frame 0

IRAVRHCS*RSSH*DLSPRLNVRRTARTHHA*M HLSPRGAGRCPDGQCLLGALLLGTWDSA*WADAQ *QDHWWRGRLLHHLLL*NWCWKTRTPGSFCGSG AYGHW*EEVGTKLKGVEHDSKLLPGERGYLDQA FLALKIPSRT*SCRRQADLRLAWAAGLGFSHTLV SQAGRRMSPSMHLATATTLGEEGPC*A*LCGFC PSR*DPKWPIPTALPPRAAHHWERGCCQQLCPW SLYHWQGDH*PSAGSDPQAGESVSWEGG

Frame 1

*GRCGTAASAVLTETCHPDST*DAPPGLTMREC ISVHVGQAGVQMGNACWELYCLEHGIQPDGQMP SDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLE PTVIGERKWGQN*RGWSMTQSFSLEREGI*TRH S*P*KFLAVLEAAEGKLI*DWPGQLVWVSLTLW YLRLAGG*VLPCIWPQLPPWGRKVPAEHSFVVFA LPDEIRNGPYRQLFHPEQLITGKEDAANNYARG HYTIGKEIIDPVLDRIRKLVRVCLGRE

Frame 2

KGGAALQLAQFSLRPVTPTQRETHRPDSPCVNA SQSTWGRQVSRWAMPAGSSIAWNMGFSLMGRCP VTRPLVEGTTPSPPSSVKLVLENTYPGQFLWIW SLRSLVRGSGDKIEGGGA*LKASPWRERVFRPGI PSPENS*PYLKLQKAS*SETGLGSWSGFLSHSG ISGWQEDESFHASGHSYHPGGGRSLLSIALWFL PFQMRSEMAHTDSSSTQSSSSLGKRMLPTTMPVV TIPLARRSLTQCWIGSASW*ECVLGGR