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Sequences

Sequence TUBA1.eAug05

ATAAGGGCGGTGCGGCACTGCAGCTAGCGCAGTTCTCACTGAGACCTGTCACCCCGACTCAACGTGAGACGCACCGCCCGGACTCACCATGATGAGATCC GAAATGGCCCATACCGACAGCTCTTCCACCCAGAGCAGCTCATCACTGGGAAAGAGGATGCTGCCAACAACTATGCCCGTGGTCACTATACCATTGGCAA GGAGATCATTGACCCAGTGCTGGATCGGATCCGCAAGCTGTCTGACCAGTGCACAGGACTTCAGGGCTTCCTGGTGTTCCACAGCTTTGGTGGGGGCACT GGCTCTGGCTTCACCTCACTCCTGATGGAGCGGCTCTCTGTTGACTATGGCAAGAAATCCAAGCTGGAATTCTCCATCTACCCAGCCCCCCAGGTGTCTA CAGCCGTGGTCGAGCCCTACAACTCTATCCTGACCACCCACACCACCCTGGAGCACTCAGACTGTGCCTTCATGGTGGACAACGAAGCAATCTATGACAT CTGCCGCCGCAACCTAGACATCGAGCGCCCAACCTACACCAACCTCAATCGCCTCATTAGCCAAATTGTCTCCTCCATCACAGCTTCTCTGCGCTTTGAC GGGGCCCTCAATGTGGACCTGACAGAGTTCCAGACCAACCTGGTGCCCTACCCTCGCATCCACTTCCCCCTGGCCACCTATGCACCAGTCATCTCTGCAG AAAAGGCATACCACGAGCAGCTGTCGGTGGCAGAGATCACCAATGCCTGCTTTGAGCCTGCCAACCAGATGGTAAAGTGTGATCCCCGGCACGGCAAGTA CATGGCCTGCTGCCTGCTGTACCGTGGAGATGTGGTGCCCAAGGATGTCAACGCTGCCATTGCCGCCATCAAGACCAAGCGCAGCATTCAGTTTGTGGAC TGGTGCCCCACAGGCTTCAAGGTTGGTATCAACTACCAGCCTCCCACTGTGGTGCCTGGGGGTGACCTGGCCAAGGTGCAGCGTGCCGTGTGCATGCTGA GCAACACGACCGCCATCGCCGAGGCCTGGGCCCGCCTGGACCACAAGTTCGACCTGATGTATGCCAAGAGGGCGTTTGTGCACTGGTATGTGGGTGAGGG CATGGAGGAGGGTGAGTTCTCCGAGGCCCGTGAGGATATGGCTGCCCTGGAGAAGGATTATGAGGAGGTGGGCATCGACTCCTATGAGGACGAGGATGAG GGAGAAGAATAAAGCAGCTGCCTGGAGCCTATTCACTATGTTTATTGCAAAATCCTTTCGAAATAAACAGTTTCCTTGCACGGTTTCTTGTTCCCTCTGA GTGCTTGAGCTTCTGCTGCCTTCCCCTTCATGCTGCTGCTGCTCTGTTTGCTGTTCATGACCCTTATCCCTTCCATCGCTGAAGGTGAGACCTGCAAAGG GGTTCTTGCCAGGCCCAGGAAGCATAACCCCAGGGACTTTAAATTGAAGTCCTAGGGTTACTCAGTATAGGGAGGAAATGAGGACCAAAATCCAGGCACA AACTGTTGAAGGATGAGTCTTCAGCTCCTTTCCAAGTAGGAGTATAATCCAGTGGTTTCTGTCTTTGAAAGTTAGACTGCTACCCTGTTCTTCCTGCTGC CTCTACATTTACCAACTCTGGAAGCAAGGCTTGGCCCAGGCTCCTCTGTGCATTATGAGGGTTTGGGAGTGGGGCTGGGCTGGCCCAGAGTTCCTGGGCA TGGAGCCACGTCATAAGCTTGGGGGATGGGAGGGAACACAGGCGATCTCCTAGAGTTGTTTGTGCCACTGCACCTCTGAGCAGCTTTGCATAGCTTCCTG TCTTGCTGCTTTGCTGGAGCTGGGGCTGTGCCATTAAAAGTCAGGTCATCTCCCCTCTGTTTGTGATTTCTTTCTGGCCTCAGCCTCTGTGCTAGCTAGT GGGAGACTCCTGCTAGGGCCCTGGGGAAAGCTTCAACTTTGCTAACAGGAGCCCCAGGCCCCATGCTGATGTCATACTGTGGGGTAGACATGGTTCGGGG GAGGAGGCTTGGATCACAAAAGAGCAGGAGATGCCAACCCTGCCCTTGCCTTTCCTCCCTGGTGATTCAGGGAACAGAGCCCTTCCCTAGGGAACAGGTT TGAAGCCCAGCTAAGACAGGGCC

TUBA1.F5 (forward primer)
TUBA1.R5 (reverse primer)
Single exon skipping

Frame 0

IRAVRHCS*RSSH*DLSPRLNVRRTARTHHDEI RNGPYRQLFHPEQLITGKEDAANNYARGHYTIGK EIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGT GSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVS TAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDI CRRNLDIERPTYTNLNRLISQIVSSITASLRFD GALNVDLTEFQTNLVPYPRIHFPLATYAPVISA EKAYHEQLSVAEITNACFEPANQMVKCDPRHGKY MACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVD WCPTGFKVGINYQPPTVVPGGDLAKVQRAVCML SNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEG MEEGEFSEAREDMAALEKDYEEVGIDSYEDEDE GEE*SSCLEPIHYVYCKILSK*TVSLHGFLFPL SA*ASAAFPFMLLLLCLLFMTLIPSIAEGETCKG VLARPRKHNPRDFKLKS*GYSV*GGNEDQNPGT NC*RMSLQLLSK*EYNPVVSVFES*TATLFFLL PLHLPTLEARLGPGSSVHYEGLGVGLGWPRVPGH GATS*AWGMGGNTGDLLELFVPLHL*AALHSFL SCCFAGAGAVPLKVRSSPLCL*FLSGLSLCAS* WETPARALGKASTLLTGAPGPMLMSYCGVDMVRG RRLGSQKSRRCQPCPCLSSLVIQGTEPFPREQV *SPAKTG

Frame 1

*GRCGTAASAVLTETCHPDST*DAPPGLTMMRS EMAHTDSSSTQSSSSLGKRMLPTTMPVVTIPLA RRSLTQCWIGSASCLTSAQDFRASWCSTALVGAL ALASPHS*WSGSLLTMARNPSWNSPSTQPPRCL QPWSSPTTLS*PPTPPWSTQTVPSWWTTKQSMT SAAAT*TSSAQPTPTSIASLAKLSPPSQLLCALT GPSMWT*QSSRPTWCPTLASTSPWPPMHQSSLQ KRHTTSSCRWQRSPMPALSLPTRW*SVIPGTAS TWPAACCTVEMWCPRMSTLPLPPSRPSAAFSLWT GAPQASRLVSTTSLPLWCLGVTWPRCSVPCAC* ATRPPSPRPGPAWTTSST*CMPRGRLCTGMWVR AWRRVSSPRPVRIWLPWRRIMRRWASTPMRTRMR EKNKAAAWSLFTMFIAKSFRNKQFPCTVSCSL* VLELLLPSPSCCCCSVCCS*PLSLPSLKVRPAK GFLPGPGSITPGTLN*SPRVTQYREEMRTKIQAQ TVEG*VFSSFPSRSIIQWFLSLKVRLLPCSSCC LYIYQLWKQGLAQAPLCIMRVWEWGWAGPEFLG MEPRHKLGGWEGTQAIS*SCLCHCTSEQLCIASC LAALLELGLCH*KSGHLPSVCDFFLASASVLAS GRLLLGPWGKLQLC*QEPQAPC*CHTVG*TWFG GGGLDHKRAGDANPALAFPPW*FREQSPSLGNRF EAQLRQG

Frame 2

KGGAALQLAQFSLRPVTPTQRETHRPDSP**DP KWPIPTALPPRAAHHWERGCCQQLCPWSLYHWQ GDH*PSAGSDPQAV*PVHRTSGLPGVPQLWWGH WLWLHLTPDGAALC*LWQEIQAGILHLPSPPGVY SRGRALQLYPDHPHHPGALRLCLHGGQRSNL*H LPPQPRHRAPNLHQPQSPH*PNCLLHHSFSAL* RGPQCGPDRVPDQPGALPSHPLPPGHLCTSHLCR KGIPRAAVGGRDHQCLL*ACQPDGKV*SPARQV HGLLPAVPWRCGAQGCQRCHCRHQDQAQHSVCG LVPHRLQGWYQLPASHCGAWG*PGQGAACRVHAE QHDRHRRGLGPPGPQVRPDVCQEGVCALVCG*G HGGG*VLRGP*GYGCPGEGL*GGGHRLL*GRG* GRRIKQLPGAYSLCLLQNPFEINSFLARFLVPSE CLSFCCLPLHAAAALFAVHDPYPFHR*R*DLQR GSCQAQEA*PQGL*IEVLGLLSIGRK*GPKSRH KLLKDESSAPFQVGV*SSGFCL*KLDCYPVLPAA STFTNSGSKAWPRLLCAL*GFGSGAGLAQSSWA WSHVISLGDGREHRRSPRVVCATAPLSSFA*LP VLLLCWSWGCAIKSQVISPLFVISFWPQPLC*LV GDSC*GPGESFNFANRSPRPHADVILWGRHGSG EEAWITKEQEMPTLPLPFLPGDSGNRALP*GTG LKPS*DRA

Sequence TUBA1.hAug05

ATAAGGGCGGTGCGGCACTGCAGCTAGCGCAGTTCTCACTGAGACCTGTCACCCCGACTCAACGTGAGACGCACCGCCCGGACTCACCATGCGTGAATGC ATCTCAGTCCACGTGGGGCAGGCAGGTGTCCAGATGGGCAATGCCTGCTGGGAGCTCTATTGCTTGGAACATGGGATTCAGCCTGATGGGCAGATGCCCA GTGACAAGACCATTGGTGGAGGGGACGACTCCTTCACCACCTTCTTCTGTGAAACTGGTGCTGGAAAACACGTACCCCGGGCAGTTTTTGTGGATCTGGA GCCTACGGTCATTGATGAGATCCGAAATGGCCCATACCGACAGCTCTTCCACCCAGAGCAGCTCATCACTGGGAAAGAGGATGCTGCCAACAACTATGCC CGTGGTCACTATACCATTGGCAAGGAGATCATTGACCCAGTGCTGGATCGGATCCGCAATCTGACCAGTGCACAGGACTTCAGGGCTTCCTGGTGTTCCA CAGCTTTGGTGGGGGCACTGGCTCTGGCTTCACCTCACTCCTGATGGAGCGGCTCTCTGTTGACTATGGCAAGAAATCCAAGCTGGAATTCTCCATCTAC CCAGCCCCCCAGGTGTCTACAGCCGTGGTCGAGCCCTACAACTCTATCCTGACCACCCACACCACCCTGGAGCACTCAGACTGTGCCTTCATGGTGGACA ACGAAGCAATCTATGACATCTGCCGCCGCAACCTAGACATCGAGCGCCCAACCTACACCAACCTCAATCGCCTCATTAGCCAAATTGTCTCCTCCATCAC AGCTTCTCTGCGCTTTGACGGGGCCCTCAATGTGGACCTGACAGAGTTCCAGACCAACCTGGTGCCCTACCCTCGCATCCACTTCCCCCTGGCCACCTAT GCACCAGTCATCTCTGCAGAAAAGGCATACCACGAGCAGCTGTCGGTGGCAGAGATCACCAATGCCTGCTTTGAGCCTGCCAACCAGATGGTAAAGTGTG ATCCCCGGCACGGCAAGTACATGGCCTGCTGCCTGCTGTACCGTGGAGATGTGGTGCCCAAGGATGTCAACGCTGCCATTGCCGCCATCAAGACCAAGCG CAGCATTCAGTTTGTGGACTGGTGCCCCACAGGCTTCAAGGTTGGTATCAACTACCAGCCTCCCACTGTGGTGCCTGGGGGTGACCTGGCCAAGGTGCAG CGTGCCGTGTGCATGCTGAGCAACACGACCGCCATCGCCGAGGCCTGGGCCCGCCTGGACCACAAGTTCGACCTGATGTATGCCAAGAGGGCGTTTGTGC ACTGGTATGTGGGTGAGGGCATGGAGGAGGGTGAGTTCTCCGAGGCCCGTGAGGATATGGCTGCCCTGGAGAAGGATTATGAGGAGGTGGGCATCGACTC CTATGAGGACGAGGATGAGGGAGAAGAATAAAGCAGCTGCCTGGAGCCTATTCACTATGTTTATTGCAAAATCCTTTCGAAATAAACAGTTTCCTTGCAC GGTTTCTTGTTCCCTCTGAGTGCTTGAGCTTCTGCTGCCTTCCCCTTCATGCTGCTGCTGCTCTGTTTGCTGTTCATGACCCTTATCCCTTCCATCGCTG AAGGTGAGACCTGCAAAGGGGTTCTTGCCAGGCCCAGGAAGCATAACCCCAGGGACTTTAAATTGAAGTCCTAGGGTTACTCAGTATAGGGAGGAAATGA GGACCAAAATCCAGGCACAAACTGTTGAAGGATGAGTCTTCAGCTCCTTTCCAAGTAGGAGTATAATCCAGTGGTTTCTGTCTTTGAAAGTTAGACTGCT ACCCTGTTCTTCCTGCTGCCTCTACATTTACCAACTCTGGAAGCAAGGCTTGGCCCAGGCTCCTCTGTGCATTATGAGGGTTTGGGAGTGGGGCTGGGCT GGCCCAGAGTTCCTGGGCATGGAGCCACGTCATAAGCTTGGGGGATGGGAGGGAACACAGGCGATCTCCTAGAGTTGTTTGTGCCACTGCACCTCTGAGC AGCTTTGCATAGCTTCCTGTCTTGCTGCTTTGCTGGAGCTGGGGCTGTGCCATTAAAAGTCAGGTCATCTCCCCTCTGTTTGTGATTTCTTTCTGGCCTC AGCCTCTGTGCTAGCTAGTGGGAGACTCCTGCTAGGGCCCTGGGGAAAGCTTCAACTTTGCTAACAGGAGCCCCAGGCCCCATGCTGATGTCATACTGTG GGGTAGACATGGTTCGGGGGAGGAGGCTTGGATCACAAAAGAGCAGGAGATGCCAACCCTGCCCTTGCCTTTCCTCCCTGGTGATTCAGGGAACAGAGCC CTTCCCTAGGGAACAGGTTTGAAGCCCAGCTAAGACAGGGCC

TUBA1.F5 (forward primer)
TUBA1.R5 (reverse primer)
Single exon skipping

Frame 0

IRAVRHCS*RSSH*DLSPRLNVRRTARTHHA*M HLSPRGAGRCPDGQCLLGALLLGTWDSA*WADAQ *QDHWWRGRLLHHLLL*NWCWKTRTPGSFCGSG AYGH**DPKWPIPTALPPRAAHHWERGCCQQLC PWSLYHWQGDH*PSAGSDPQSDQCTGLQGFLVFH SFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIY PAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVD NEAIYDICRRNLDIERPTYTNLNRLISQIVSSIT ASLRFDGALNVDLTEFQTNLVPYPRIHFPLATY APVISAEKAYHEQLSVAEITNACFEPANQMVKC DPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKR SIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQ RAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFV HWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDS YEDEDEGEE*SSCLEPIHYVYCKILSK*TVSLH GFLFPLSA*ASAAFPFMLLLLCLLFMTLIPSIA EGETCKGVLARPRKHNPRDFKLKS*GYSV*GGNE DQNPGTNC*RMSLQLLSK*EYNPVVSVFES*TA TLFFLLPLHLPTLEARLGPGSSVHYEGLGVGLG WPRVPGHGATS*AWGMGGNTGDLLELFVPLHL*A ALHSFLSCCFAGAGAVPLKVRSSPLCL*FLSGL SLCAS*WETPARALGKASTLLTGAPGPMLMSYC GVDMVRGRRLGSQKSRRCQPCPCLSSLVIQGTEP FPREQV*SPAKTG

Frame 1

*GRCGTAASAVLTETCHPDST*DAPPGLTMREC ISVHVGQAGVQMGNACWELYCLEHGIQPDGQMP SDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLE PTVIDEIRNGPYRQLFHPEQLITGKEDAANNYA RGHYTIGKEIIDPVLDRIRNLTSAQDFRASWCS TALVGALALASPHS*WSGSLLTMARNPSWNSPST QPPRCLQPWSSPTTLS*PPTPPWSTQTVPSWWT TKQSMTSAAAT*TSSAQPTPTSIASLAKLSPPS QLLCALTGPSMWT*QSSRPTWCPTLASTSPWPPM HQSSLQKRHTTSSCRWQRSPMPALSLPTRW*SV IPGTASTWPAACCTVEMWCPRMSTLPLPPSRPS AAFSLWTGAPQASRLVSTTSLPLWCLGVTWPRCS VPCAC*ATRPPSPRPGPAWTTSST*CMPRGRLC TGMWVRAWRRVSSPRPVRIWLPWRRIMRRWAST PMRTRMREKNKAAAWSLFTMFIAKSFRNKQFPCT VSCSL*VLELLLPSPSCCCCSVCCS*PLSLPSL KVRPAKGFLPGPGSITPGTLN*SPRVTQYREEM RTKIQAQTVEG*VFSSFPSRSIIQWFLSLKVRLL PCSSCCLYIYQLWKQGLAQAPLCIMRVWEWGWA GPEFLGMEPRHKLGGWEGTQAIS*SCLCHCTSE QLCIASCLAALLELGLCH*KSGHLPSVCDFFLAS ASVLASGRLLLGPWGKLQLC*QEPQAPC*CHTV G*TWFGGGGLDHKRAGDANPALAFPPW*FREQS PSLGNRFEAQLRQG

Frame 2

KGGAALQLAQFSLRPVTPTQRETHRPDSPCVNA SQSTWGRQVSRWAMPAGSSIAWNMGFSLMGRCP VTRPLVEGTTPSPPSSVKLVLENTYPGQFLWIW SLRSLMRSEMAHTDSSSTQSSSSLGKRMLPTTMP VVTIPLARRSLTQCWIGSAI*PVHRTSGLPGVP QLWWGHWLWLHLTPDGAALC*LWQEIQAGILHL PSPPGVYSRGRALQLYPDHPHHPGALRLCLHGGQ RSNL*HLPPQPRHRAPNLHQPQSPH*PNCLLHH SFSAL*RGPQCGPDRVPDQPGALPSHPLPPGHL CTSHLCRKGIPRAAVGGRDHQCLL*ACQPDGKV* SPARQVHGLLPAVPWRCGAQGCQRCHCRHQDQA QHSVCGLVPHRLQGWYQLPASHCGAWG*PGQGA ACRVHAEQHDRHRRGLGPPGPQVRPDVCQEGVCA LVCG*GHGGG*VLRGP*GYGCPGEGL*GGGHRL L*GRG*GRRIKQLPGAYSLCLLQNPFEINSFLA RFLVPSECLSFCCLPLHAAAALFAVHDPYPFHR* R*DLQRGSCQAQEA*PQGL*IEVLGLLSIGRK* GPKSRHKLLKDESSAPFQVGV*SSGFCL*KLDC YPVLPAASTFTNSGSKAWPRLLCAL*GFGSGAGL AQSSWAWSHVISLGDGREHRRSPRVVCATAPLS SFA*LPVLLLCWSWGCAIKSQVISPLFVISFWP QPLC*LVGDSC*GPGESFNFANRSPRPHADVILW GRHGSGEEAWITKEQEMPTLPLPFLPGDSGNRA LP*GTGLKPS*DRA

Sequence TUBA1.aAug05

ATAAGGGCGGTGCGGCACTGCAGCTAGCGCAGTTCTCACTGAGACCTGTCACCCCGACTCAACGTGAGACGCACCGCCCGGACTCACCATGCGTGAATGC ATCTCAGTCCACGTGGGGCAGGCAGGTGTCCAGATGGGCAATGCCTGCTGGGAGCTCTATTGCTTGGAACATGGGATTCAGCCTGATGGGCAGATGCCCA GTGACAAGACCATTGGTGGAGGGGACGACTCCTTCACCACCTTCTTCTGTGAAACTGGTGCTGGAAAACACGTACCCCGGGCAGTTTTTGTGGATCTGGA GCCTACGGTCATTGATGAGATCCGAAATGGCCCATACCGACAGCTCTTCCACCCAGAGCAGCTCATCACTGGGAAAGAGGATGCTGCCAACAACTATGCC CGTGGTCACTATACCATTGGCAAGGAGATCATTGACCCAGTGCTGGATCGGATCCGCAAGCTGTCTGACCAGTGCACAGGACTTCAGGGCTTCCTGGTGT TCCACAGCTTTGGTGGGGGCACTGGCTCTGGCTTCACCTCACTCCTGATGGAGCGGCTCTCTGTTGACTATGGCAAGAAATCCAAGCTGGAATTCTCCAT CTACCCAGCCCCCCAGGTGTCTACAGCCGTGGTCGAGCCCTACAACTCTATCCTGACCACCCACACCACCCTGGAGCACTCAGACTGTGCCTTCATGGTG GACAACGAAGCAATCTATGACATCTGCCGCCGCAACCTAGACATCGAGCGCCCAACCTACACCAACCTCAATCGCCTCATTAGCCAAATTGTCTCCTCCA TCACAGCTTCTCTGCGCTTTGACGGGGCCCTCAATGTGGACCTGACAGAGTTCCAGACCAACCTGGTGCCCTACCCTCGCATCCACTTCCCCCTGGCCAC CTATGCACCAGTCATCTCTGCAGAAAAGGCATACCACGAGCAGCTGTCGGTGGCAGAGATCACCAATGCCTGCTTTGAGCCTGCCAACCAGATGGTAAAG TGTGATCCCCGGCACGGCAAGTACATGGCCTGCTGCCTGCTGTACCGTGGAGATGTGGTGCCCAAGGATGTCAACGCTGCCATTGCCGCCATCAAGACCA AGCGCAGCATTCAGTTTGTGGACTGGTGCCCCACAGGCTTCAAGGTTGGTATCAACTACCAGCCTCCCACTGTGGTGCCTGGGGGTGACCTGGCCAAGGT GCAGCGTGCCGTGTGCATGCTGAGCAACACGACCGCCATCGCCGAGGCCTGGGCCCGCCTGGACCACAAGTTCGACCTGATGTATGCCAAGAGGGCGTTT GTGCACTGGTATGTGGGTGAGGGCATGGAGGAGGGTGAGTTCTCCGAGGCCCGTGAGGATATGGCTGCCCTGGAGAAGGATTATGAGGAGGTGGGCATCG ACTCCTATGAGGACGAGGATGAGGGAGAAGAATAAAGCAGCTGCCTGGAGCCTATTCACTATGTTTATTGCAAAATCCTTTCGAAATAAACAGTTTCCTT GCACGGTTTCTTGTTCCCTCTGAGTGCTTGAGCTTCTGCTGCCTTCCCCTTCATGCTGCTGCTGCTCTGTTTGCTGTTCATGACCCTTATCCCTTCCATC GCTGAAGGTGAGACCTGCAAAGGGGTTCTTGCCAGGCCCAGGAAGCATAACCCCAGGGACTTTAAATTGAAGTCCTAGGGTTACTCAGTATAGGGAGGAA ATGAGGACCAAAATCCAGGCACAAACTGTTGAAGGATGAGTCTTCAGCTCCTTTCCAAGTAGGAGTATAATCCAGTGGTTTCTGTCTTTGAAAGTTAGAC TGCTACCCTGTTCTTCCTGCTGCCTCTACATTTACCAACTCTGGAAGCAAGGCTTGGCCCAGGCTCCTCTGTGCATTATGAGGGTTTGGGAGTGGGGCTG GGCTGGCCCAGAGTTCCTGGGCATGGAGCCACGTCATAAGCTTGGGGGATGGGAGGGAACACAGGCGATCTCCTAGAGTTGTTTGTGCCACTGCACCTCT GAGCAGCTTTGCATAGCTTCCTGTCTTGCTGCTTTGCTGGAGCTGGGGCTGTGCCATTAAAAGTCAGGTCATCTCCCCTCTGTTTGTGATTTCTTTCTGG CCTCAGCCTCTGTGCTAGCTAGTGGGAGACTCCTGCTAGGGCCCTGGGGAAAGCTTCAACTTTGCTAACAGGAGCCCCAGGCCCCATGCTGATGTCATAC TGTGGGGTAGACATGGTTCGGGGGAGGAGGCTTGGATCACAAAAGAGCAGGAGATGCCAACCCTGCCCTTGCCTTTCCTCCCTGGTGATTCAGGGAACAG AGCCCTTCCCTAGGGAACAGGTTTGAAGCCCAGCTAAGACAGGGCC

TUBA1.F5 (forward primer)
TUBA1.R5 (reverse primer)
Single exon skipping

Frame 0

IRAVRHCS*RSSH*DLSPRLNVRRTARTHHA*M HLSPRGAGRCPDGQCLLGALLLGTWDSA*WADAQ *QDHWWRGRLLHHLLL*NWCWKTRTPGSFCGSG AYGH**DPKWPIPTALPPRAAHHWERGCCQQLC PWSLYHWQGDH*PSAGSDPQAV*PVHRTSGLPGV PQLWWGHWLWLHLTPDGAALC*LWQEIQAGILH LPSPPGVYSRGRALQLYPDHPHHPGALRLCLHG GQRSNL*HLPPQPRHRAPNLHQPQSPH*PNCLLH HSFSAL*RGPQCGPDRVPDQPGALPSHPLPPGH LCTSHLCRKGIPRAAVGGRDHQCLL*ACQPDGK V*SPARQVHGLLPAVPWRCGAQGCQRCHCRHQDQ AQHSVCGLVPHRLQGWYQLPASHCGAWG*PGQG AACRVHAEQHDRHRRGLGPPGPQVRPDVCQEGV CALVCG*GHGGG*VLRGP*GYGCPGEGL*GGGHR LL*GRG*GRRIKQLPGAYSLCLLQNPFEINSFL ARFLVPSECLSFCCLPLHAAAALFAVHDPYPFH R*R*DLQRGSCQAQEA*PQGL*IEVLGLLSIGRK *GPKSRHKLLKDESSAPFQVGV*SSGFCL*KLD CYPVLPAASTFTNSGSKAWPRLLCAL*GFGSGA GLAQSSWAWSHVISLGDGREHRRSPRVVCATAPL SSFA*LPVLLLCWSWGCAIKSQVISPLFVISFW PQPLC*LVGDSC*GPGESFNFANRSPRPHADVI LWGRHGSGEEAWITKEQEMPTLPLPFLPGDSGNR ALP*GTGLKPS*DRA

Frame 1

*GRCGTAASAVLTETCHPDST*DAPPGLTMREC ISVHVGQAGVQMGNACWELYCLEHGIQPDGQMP SDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLE PTVIDEIRNGPYRQLFHPEQLITGKEDAANNYA RGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLV FHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSI YPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMV DNEAIYDICRRNLDIERPTYTNLNRLISQIVSS ITASLRFDGALNVDLTEFQTNLVPYPRIHFPLAT YAPVISAEKAYHEQLSVAEITNACFEPANQMVK CDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKT KRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKV QRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAF VHWYVGEGMEEGEFSEAREDMAALEKDYEEVGI DSYEDEDEGEE*SSCLEPIHYVYCKILSK*TVSL HGFLFPLSA*ASAAFPFMLLLLCLLFMTLIPSI AEGETCKGVLARPRKHNPRDFKLKS*GYSV*GG NEDQNPGTNC*RMSLQLLSK*EYNPVVSVFES*T ATLFFLLPLHLPTLEARLGPGSSVHYEGLGVGL GWPRVPGHGATS*AWGMGGNTGDLLELFVPLHL *AALHSFLSCCFAGAGAVPLKVRSSPLCL*FLSG LSLCAS*WETPARALGKASTLLTGAPGPMLMSY CGVDMVRGRRLGSQKSRRCQPCPCLSSLVIQGT EPFPREQV*SPAKTG

Frame 2

KGGAALQLAQFSLRPVTPTQRETHRPDSPCVNA SQSTWGRQVSRWAMPAGSSIAWNMGFSLMGRCP VTRPLVEGTTPSPPSSVKLVLENTYPGQFLWIW SLRSLMRSEMAHTDSSSTQSSSSLGKRMLPTTMP VVTIPLARRSLTQCWIGSASCLTSAQDFRASWC STALVGALALASPHS*WSGSLLTMARNPSWNSP STQPPRCLQPWSSPTTLS*PPTPPWSTQTVPSWW TTKQSMTSAAAT*TSSAQPTPTSIASLAKLSPP SQLLCALTGPSMWT*QSSRPTWCPTLASTSPWP PMHQSSLQKRHTTSSCRWQRSPMPALSLPTRW*S VIPGTASTWPAACCTVEMWCPRMSTLPLPPSRP SAAFSLWTGAPQASRLVSTTSLPLWCLGVTWPR CSVPCAC*ATRPPSPRPGPAWTTSST*CMPRGRL CTGMWVRAWRRVSSPRPVRIWLPWRRIMRRWAS TPMRTRMREKNKAAAWSLFTMFIAKSFRNKQFP CTVSCSL*VLELLLPSPSCCCCSVCCS*PLSLPS LKVRPAKGFLPGPGSITPGTLN*SPRVTQYREE MRTKIQAQTVEG*VFSSFPSRSIIQWFLSLKVR LLPCSSCCLYIYQLWKQGLAQAPLCIMRVWEWGW AGPEFLGMEPRHKLGGWEGTQAIS*SCLCHCTS EQLCIASCLAALLELGLCH*KSGHLPSVCDFFL ASASVLASGRLLLGPWGKLQLC*QEPQAPC*CHT VG*TWFGGGGLDHKRAGDANPALAFPPW*FREQ SPSLGNRFEAQLRQG