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Sequences

Sequence TUBA1.eAug05

ATAAGGGCGGTGCGGCACTGCAGCTAGCGCAGTTCTCACTGAGACCTGTCACCCCGACTCAACGTGAGACGCACCGCCCGGACTCACCATGATGAGATCC
GAAATGGCCCATACCGACAGCTCTTCCACCCAGAGCAGCTCATCACTGGGAAAGAGGATGCTGCCAACAACTATGCCCGTGGTCACTATACCATTGGCAA
GGAGATCATTGACCCAGTGCTGGATCGGATCCGCAAGCTGTCTGACCAGTGCACAGGACTTCAGGGCTTCCTGGTGTTCCACAGCTTTGGTGGGGGCACT
GGCTCTGGCTTCACCTCACTCCTGATGGAGCGGCTCTCTGTTGACTATGGCAAGAAATCCAAGCTGGAATTCTCCATCTACCCAGCCCCCCAGGTGTCTA
CAGCCGTGGTCGAGCCCTACAACTCTATCCTGACCACCCACACCACCCTGGAGCACTCAGACTGTGCCTTCATGGTGGACAACGAAGCAATCTATGACAT
CTGCCGCCGCAACCTAGACATCGAGCGCCCAACCTACACCAACCTCAATCGCCTCATTAGCCAAATTGTCTCCTCCATCACAGCTTCTCTGCGCTTTGAC
GGGGCCCTCAATGTGGACCTGACAGAGTTCCAGACCAACCTGGTGCCCTACCCTCGCATCCACTTCCCCCTGGCCACCTATGCACCAGTCATCTCTGCAG
AAAAGGCATACCACGAGCAGCTGTCGGTGGCAGAGATCACCAATGCCTGCTTTGAGCCTGCCAACCAGATGGTAAAGTGTGATCCCCGGCACGGCAAGTA
CATGGCCTGCTGCCTGCTGTACCGTGGAGATGTGGTGCCCAAGGATGTCAACGCTGCCATTGCCGCCATCAAGACCAAGCGCAGCATTCAGTTTGTGGAC
TGGTGCCCCACAGGCTTCAAGGTTGGTATCAACTACCAGCCTCCCACTGTGGTGCCTGGGGGTGACCTGGCCAAGGTGCAGCGTGCCGTGTGCATGCTGA
GCAACACGACCGCCATCGCCGAGGCCTGGGCCCGCCTGGACCACAAGTTCGACCTGATGTATGCCAAGAGGGCGTTTGTGCACTGGTATGTGGGTGAGGG
CATGGAGGAGGGTGAGTTCTCCGAGGCCCGTGAGGATATGGCTGCCCTGGAGAAGGATTATGAGGAGGTGGGCATCGACTCCTATGAGGACGAGGATGAG
GGAGAAGAATAAAGCAGCTGCCTGGAGCCTATTCACTATGTTTATTGCAAAATCCTTTCGAAATAAACAGTTTCCTTGCACGGTTTCTTGTTCCCTCTGA
GTGCTTGAGCTTCTGCTGCCTTCCCCTTCATGCTGCTGCTGCTCTGTTTGCTGTTCATGACCCTTATCCCTTCCATCGCTGAAGGTGAGACCTGCAAAGG
GGTTCTTGCCAGGCCCAGGAAGCATAACCCCAGGGACTTTAAATTGAAGTCCTAGGGTTACTCAGTATAGGGAGGAAATGAGGACCAAAATCCAGGCACA
AACTGTTGAAGGATGAGTCTTCAGCTCCTTTCCAAGTAGGAGTATAATCCAGTGGTTTCTGTCTTTGAAAGTTAGACTGCTACCCTGTTCTTCCTGCTGC
CTCTACATTTACCAACTCTGGAAGCAAGGCTTGGCCCAGGCTCCTCTGTGCATTATGAGGGTTTGGGAGTGGGGCTGGGCTGGCCCAGAGTTCCTGGGCA
TGGAGCCACGTCATAAGCTTGGGGGATGGGAGGGAACACAGGCGATCTCCTAGAGTTGTTTGTGCCACTGCACCTCTGAGCAGCTTTGCATAGCTTCCTG
TCTTGCTGCTTTGCTGGAGCTGGGGCTGTGCCATTAAAAGTCAGGTCATCTCCCCTCTGTTTGTGATTTCTTTCTGGCCTCAGCCTCTGTGCTAGCTAGT
GGGAGACTCCTGCTAGGGCCCTGGGGAAAGCTTCAACTTTGCTAACAGGAGCCCCAGGCCCCATGCTGATGTCATACTGTGGGGTAGACATGGTTCGGGG
GAGGAGGCTTGGATCACAAAAGAGCAGGAGATGCCAACCCTGCCCTTGCCTTTCCTCCCTGGTGATTCAGGGAACAGAGCCCTTCCCTAGGGAACAGGTT
TGAAGCCCAGCTAAGACAGGGCC
  • TUBA1.F5 (forward primer)
  • TUBA1.R5 (reverse primer)
  • Alternative acceptor

Frame 0

IRAVRHCS*RSSH*DLSPRLNVRRTARTHHDEI
RNGPYRQLFHPEQLITGKEDAANNYARGHYTIGK
EIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGT
GSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVS
TAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDI
CRRNLDIERPTYTNLNRLISQIVSSITASLRFD
GALNVDLTEFQTNLVPYPRIHFPLATYAPVISA
EKAYHEQLSVAEITNACFEPANQMVKCDPRHGKY
MACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVD
WCPTGFKVGINYQPPTVVPGGDLAKVQRAVCML
SNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEG
MEEGEFSEAREDMAALEKDYEEVGIDSYEDEDE
GEE*SSCLEPIHYVYCKILSK*TVSLHGFLFPL
SA*ASAAFPFMLLLLCLLFMTLIPSIAEGETCKG
VLARPRKHNPRDFKLKS*GYSV*GGNEDQNPGT
NC*RMSLQLLSK*EYNPVVSVFES*TATLFFLL
PLHLPTLEARLGPGSSVHYEGLGVGLGWPRVPGH
GATS*AWGMGGNTGDLLELFVPLHL*AALHSFL
SCCFAGAGAVPLKVRSSPLCL*FLSGLSLCAS*
WETPARALGKASTLLTGAPGPMLMSYCGVDMVRG
RRLGSQKSRRCQPCPCLSSLVIQGTEPFPREQV
*SPAKTG

Frame 1

*GRCGTAASAVLTETCHPDST*DAPPGLTMMRS
EMAHTDSSSTQSSSSLGKRMLPTTMPVVTIPLA
RRSLTQCWIGSASCLTSAQDFRASWCSTALVGAL
ALASPHS*WSGSLLTMARNPSWNSPSTQPPRCL
QPWSSPTTLS*PPTPPWSTQTVPSWWTTKQSMT
SAAAT*TSSAQPTPTSIASLAKLSPPSQLLCALT
GPSMWT*QSSRPTWCPTLASTSPWPPMHQSSLQ
KRHTTSSCRWQRSPMPALSLPTRW*SVIPGTAS
TWPAACCTVEMWCPRMSTLPLPPSRPSAAFSLWT
GAPQASRLVSTTSLPLWCLGVTWPRCSVPCAC*
ATRPPSPRPGPAWTTSST*CMPRGRLCTGMWVR
AWRRVSSPRPVRIWLPWRRIMRRWASTPMRTRMR
EKNKAAAWSLFTMFIAKSFRNKQFPCTVSCSL*
VLELLLPSPSCCCCSVCCS*PLSLPSLKVRPAK
GFLPGPGSITPGTLN*SPRVTQYREEMRTKIQAQ
TVEG*VFSSFPSRSIIQWFLSLKVRLLPCSSCC
LYIYQLWKQGLAQAPLCIMRVWEWGWAGPEFLG
MEPRHKLGGWEGTQAIS*SCLCHCTSEQLCIASC
LAALLELGLCH*KSGHLPSVCDFFLASASVLAS
GRLLLGPWGKLQLC*QEPQAPC*CHTVG*TWFG
GGGLDHKRAGDANPALAFPPW*FREQSPSLGNRF
EAQLRQG

Frame 2

KGGAALQLAQFSLRPVTPTQRETHRPDSP**DP
KWPIPTALPPRAAHHWERGCCQQLCPWSLYHWQ
GDH*PSAGSDPQAV*PVHRTSGLPGVPQLWWGH
WLWLHLTPDGAALC*LWQEIQAGILHLPSPPGVY
SRGRALQLYPDHPHHPGALRLCLHGGQRSNL*H
LPPQPRHRAPNLHQPQSPH*PNCLLHHSFSAL*
RGPQCGPDRVPDQPGALPSHPLPPGHLCTSHLCR
KGIPRAAVGGRDHQCLL*ACQPDGKV*SPARQV
HGLLPAVPWRCGAQGCQRCHCRHQDQAQHSVCG
LVPHRLQGWYQLPASHCGAWG*PGQGAACRVHAE
QHDRHRRGLGPPGPQVRPDVCQEGVCALVCG*G
HGGG*VLRGP*GYGCPGEGL*GGGHRLL*GRG*
GRRIKQLPGAYSLCLLQNPFEINSFLARFLVPSE
CLSFCCLPLHAAAALFAVHDPYPFHR*R*DLQR
GSCQAQEA*PQGL*IEVLGLLSIGRK*GPKSRH
KLLKDESSAPFQVGV*SSGFCL*KLDCYPVLPAA
STFTNSGSKAWPRLLCAL*GFGSGAGLAQSSWA
WSHVISLGDGREHRRSPRVVCATAPLSSFA*LP
VLLLCWSWGCAIKSQVISPLFVISFWPQPLC*LV
GDSC*GPGESFNFANRSPRPHADVILWGRHGSG
EEAWITKEQEMPTLPLPFLPGDSGNRALP*GTG
LKPS*DRA

Sequence TUBA1.jAug05

ATAAGGGCGGTGCGGCACTGCAGCTAGCGCAGTTCTCACTGAGACCTGTCACCCCGACTCAACGTGAGACGCACCGCCCGGACTCACCATGCGTGAATGC
ATCTCAGTCCACGTGGGGCAGGCAGGTGTCCAGATGGGCAATGCCTGCTGGGAGCTCTATTGCTTGGAACATGGGATTCAGCCTGATGGGCAGATGCCCA
GTGACAAGACCATTGGTGGAGGGGACGACTCCTTCACCACCTTCTTCTGTGAAACTGGTGCTGGAAAACACGTACCCCGGGCAGTTTTTGTGGATCTGGA
GCCTACGGTCATTGGTGAGAGGAAGTGGGGACAAAATTGAAGGGGGTGGAGCATGACTCAAAGCTTCTCCCTGGAGAGAGAGGGTATTTAGACCAGGCAT
TCCTAGCCCTGAAAATTCCTAGCCGTACTTGAAGCTGCAGAAGGCAAGCTGATCTGAGACTGGCCTGGGCAGCTGGTCTGGGTTTCTCTCACACTCTGGT
ATCTCAGGCTGGCAGGAGGATGAGTCCTTCCATGCATCTGGCCACAGCTACCACCCTGGGGGAGGAAGGTCCCTGCTGAGCATAGCTTTGTGGTTTTTGC
CCTTCCAGATGAGATCCGAAATGGCCCATACCGACAGCTCTTCCACCCAGAGCAGCTCATCACTGGGAAAGAGGATGCTGCCAACAACTATGCCCGTGGT
CACTATACCATTGGCAAGGAGATCATTGACCCAGTGCTGGATCGGATCCGCAAGCTGGTGAGAGTGTGTCTTGGGAGGGAGGG
  • TUBA1.F5 (forward primer)
  • TUBA1.R5 (reverse primer)
  • Alternative acceptor

Frame 0

IRAVRHCS*RSSH*DLSPRLNVRRTARTHHA*M
HLSPRGAGRCPDGQCLLGALLLGTWDSA*WADAQ
*QDHWWRGRLLHHLLL*NWCWKTRTPGSFCGSG
AYGHW*EEVGTKLKGVEHDSKLLPGERGYLDQA
FLALKIPSRT*SCRRQADLRLAWAAGLGFSHTLV
SQAGRRMSPSMHLATATTLGEEGPC*A*LCGFC
PSR*DPKWPIPTALPPRAAHHWERGCCQQLCPW
SLYHWQGDH*PSAGSDPQAGESVSWEGG

Frame 1

*GRCGTAASAVLTETCHPDST*DAPPGLTMREC
ISVHVGQAGVQMGNACWELYCLEHGIQPDGQMP
SDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLE
PTVIGERKWGQN*RGWSMTQSFSLEREGI*TRH
S*P*KFLAVLEAAEGKLI*DWPGQLVWVSLTLW
YLRLAGG*VLPCIWPQLPPWGRKVPAEHSFVVFA
LPDEIRNGPYRQLFHPEQLITGKEDAANNYARG
HYTIGKEIIDPVLDRIRKLVRVCLGRE

Frame 2

KGGAALQLAQFSLRPVTPTQRETHRPDSPCVNA
SQSTWGRQVSRWAMPAGSSIAWNMGFSLMGRCP
VTRPLVEGTTPSPPSSVKLVLENTYPGQFLWIW
SLRSLVRGSGDKIEGGGA*LKASPWRERVFRPGI
PSPENS*PYLKLQKAS*SETGLGSWSGFLSHSG
ISGWQEDESFHASGHSYHPGGGRSLLSIALWFL
PFQMRSEMAHTDSSSTQSSSSLGKRMLPTTMPVV
TIPLARRSLTQCWIGSASW*ECVLGGR

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