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Sequences

Sequence TSSC4.fAug05

GGCGTGGAGGAGTAGAGGGGCGAGCGCATGCGCACAGGACTACACGTCCCGACAGGCGTCGGGAGCGGCGGCCCAGTTCCTTGTGGGAGCTGTAGTTCTG CAGGCGCGGAAGCCGTGGTGCTCGGCCGGCAGAGCACTCGGTTTCCCAGAGGGCTGAGCGCGCCGCACGGAGGTGCGGCGCCGACCAAGATGGAGACTGC CGAGCAGCCTTGAGCCGGTAGGTTTGTGGTGAGGGAGGACGGGCCGCGCGGGCCGGCCGAGCCTCCGGGAGGTCACCGAGCGCAGCTTTAATACCTGAGC TCGAAGGCCCCGCTGTGCTCGCCGACCCCCGTACCTCGCGGCCGGGCCCTTGGGACCCACAGCATCCTTGTGAGGCCCGGAGGCCTGTCCAGCCCGACTG GACAGTGCCGAGGGGCACCGAGAGCCAGCTTGGCACCGAGAGTTCGTTTGTTCTCTGGCGGGGAGGTCTTGCTGGCACATATAGTGGAGAAAGGCCGGGC TCTGCGTTCATGTGGAGAAAGAGACGGCTTCCTTCAGCCTACGGACATGAAGGAGTCAACTCTACCTTCCACTCGTTGCCGGCTTTCGCCGAGAACCCCG AGAAACGGACTACCGGAGTCCCTATCTTGCAGCCCGATCCCCGCTACCCGTCGGAGTGCCCCGCTGACCAGGCTGCTTCTGGCCGCGGCGGCGTTCCGCT GCAGAGGACGGGAGTGCGAATCTGGGAAGCAGGGTTCTGGTTGAACTCCAGCTTCGTCTGCAACATACTGTGTGACTTGGGCAAATTATTTCCCCCGCCC CGTTCCTGCCAGCTTTAAAACGGTCATCAGTGGGGGGTGCTGCGTATCCCCTTTCACTGGGGTGGCTTCTTCACTGAGGAGAGTCGCGCCTCAGAGGAAC TGAGGTCCTGCCTGTGTTCGACCTGGTGGGGGGCACTAAGAGCCCCTGATAGTACCCCTGACCCCATCCTTATTGGGTGCACAAGACACAGGTCACTCTG GGCGGGCAAGGAGTTTTGGTAGCAGGAGAGGAGTCGGTGGATGGATGGCTGAGGACAGTGCAGAAGGGTGTGGCTGGGCCGTCTTTTTTTGCCTGGAAAT TCAAGTTCTGAGGCACCCAGTCACTCCAGCACTAAATGGGTGCAGGAGGCAGCACTTGTCTGCCCAGCTGGAAAGGCAGGGTATGTGCTGAGTGTTACAG GTGGAAGGCCACTGGAGGTCGCTCCAGGAGCCGCGGGGATTTACCTCTGCCTAACAGGGCTGCTCAAGGTGATGGTCGACACCCCACTTTCCTGAGAGCT TGACCCTCAGATGCCAGGGCCTTGGCTGCAGATTCCTTGGGAGCTCCCGGGGATCTTCCAGCAAATAGGAGCAAATCTTTTCCCCGTGGATCAGGAAGGT GCACGCTCTTTGTGGAATACGACTGCTCACCCCGCACAGCAAGCAGCTTATAAGTGGCCCTCCTGCCTGATTTCAGCCCTGGGTTCAAGCCCTGGGTGGC TGCTTACTACCAAAATCGCTCAGTAGCTCCAAGCCTGCCTGCAGAGGGTTGGCACCATTAAATGAGTTGAGCAGCTGAACAGAGGCCATGCCGGGGCACT CCGAGGCCTGAGACGACCACGCCTGTGCCGCTGAGGACCTTCATCAGGGCTCCGTCCACTTGGCCCGCTTGGCTGTCCAATCACACTCCAGTGTCAACCA CTGGCACCCAGCAGCCAAGAGAGGTGTGGCGTGGCCCTGGGGACGCATGGCTGAGGCAGGAACAGGCCTCCTCCCAGCCACGGTGCAGCCATTCCATCTG AGAGGCATGAGCTCCACCTTCTCCCAGCGCAGCCGTGACATCTTTGACTGCCTGGAGGGGGCGGCCAGACGGGCTCCATCCTCTGTGGCCCACACCAGCA TGAGTGACAACGGAGGCTTCAAGCGGCCCCTAGCGCCCTCAGGCCGGTCTCCAGTGGAAGGCCTGGGCAGGGCCCATCGGAGCCCTGCCTCACCAAGGGT GCCTCCGGTCCCCGACTACGTGGCACACCCCGAGCGCTGGACCAAGTACAGCCTGGAAGATGTGACCGAGGTCAGCGAGCAGAGCAATCAGGCCACCGCC CTGGCCTTCCTGGGCTCCCAGAGCCTGGCTGCCCCCACTGACTGCGTGTCCTCCTTCAACCAGGATCCCTCCAGCTGTGGGGAGGGGAGGGTCATCTTCA CCAAACCAGTCCGAGGGGTCGAAGCCAGACACGAGAGGAAGAGGGTCCTGGGGAAGGTGGGAGAGCCAGGCAGGGGCGGCCTTGGGAATCCTGCCACAGA CAGGGGCGAGGGCCCTGTGGAGCTGGCCCATCTGGCCGGGCCCGGGAGCCCAGAGGCTGAGGAGTGGGGCAGCCACCATGGAGGCCTGCAGGAGGTGGAG GCACTGTCAGGGTCTGTCCACAGTGGGTCTGTGCCAGGTCTCCCGCCGGTGGAAACTGTTGGCTTCCATGGCAGCAGGAAGCGGAGTCGAGACCACTTCC GGAACAAGAGCAGCAGCCCCGAGGACCCAGGTGCTGAGGTCTGAGAGGGAGATGGCCCAGCCTGACCCCACTGGCCACTGCCATCCTGCTGCCTTCCCAG TGGGGCTGGTCAGGGGGCAGCCTGGCCACTGCCTAGCTGGAATGGGAGGAAGCCTGCAGGTGGCACCGGTGGCCCTGGCTGCAGTTCTGGGCAGCATCCT CCCAAGCAGAGACCTTGCTGAAGCTCCTGGGGTGTGGGGTGTGGGCTGGAAGCACTGGCTCCCTGGTAGGGACAATAAAGGTTTTGGGTCTTTCTGAGA

TSSC4.F10 (forward primer)
TSSC4.R7 (reverse primer)
Intron retention

Frame 0

GVEE*RGERMRTGLHVPTGVGSGGPVPCGSCSS AGAEAVVLGRQSTRFPRGLSAPHGGAAPTKMETA EQP*AGRFVVREDGPRGPAEPPGGHRAQL*YLS SKAPLCSPTPVPRGRALGTHSILVRPGGLSSPT GQCRGAPRASLAPRVRLFSGGEVLLAHIVEKGRA LRSCGERDGFLQPTDMKESTLPSTRCRLSPRTP RNGLPESLSCSPIPATRRSAPLTRLLLAAAAFR CRGRECESGKQGSG*TPASSATYCVTWANYFPRP VPASFKTVISGGCCVSPFTGVASSLRRVAPQRN *GPACVRPGGGH*EPLIVPLTPSLLGAQDTGHS GRARSFGSRRGVGGWMAEDSAEGCGWAVFFCLEI QVLRHPVTPALNGCRRQHLSAQLERQGMC*VLQ VEGHWRSLQEPRGFTSA*QGCSR*WSTPHFPES LTLRCQGLGCRFLGSSRGSSSK*EQIFSPWIRKV HALCGIRLLTPHSKQLISGPPA*FQPWVQALGG CLLPKSLSSSKPACRGLAPLNELSS*TEAMPGH SEA*DDHACAAEDLHQGSVHLARLAVQSHSSVNH WHPAAKRGVAWPWGRMAEAGTGLLPATVQPFHL RGMSSTFSQRSRDIFDCLEGAARRAPSSVAHTS MSDNGGFKRPLAPSGRSPVEGLGRAHRSPASPRV PPVPDYVAHPERWTKYSLEDVTEVSEQSNQATA LAFLGSQSLAAPTDCVSSFNQDPSSCGEGRVIF TKPVRGVEARHERKRVLGKVGEPGRGGLGNPATD RGEGPVELAHLAGPGSPEAEEWGSHHGGLQEVE ALSGSVHSGSVPGLPPVETVGFHGSRKRSRDHF RNKSSSPEDPGAEV*EGDGPA*PHWPLPSCCLPS GAGQGAAWPLPSWNGRKPAGGTGGPGCSSGQHP PKQRPC*SSWGVGCGLEALAPW*GQ*RFWVFLR

Frame 1

AWRSRGASACAQDYTSRQASGAAAQFLVGAVVL QARKPWCSAGRALGFPEG*ARRTEVRRRPRWRL PSSLEPVGLW*GRTGRAGRPSLREVTERSFNT*A RRPRCARRPPYLAAGPLGPTASL*GPEACPARL DSAEGHREPAWHREFVCSLAGRSCWHI*WRKAG LCVHVEKETASFSLRT*RSQLYLPLVAGFRREPR ETDYRSPYLAARSPLPVGVPR*PGCFWPRRRSA AEDGSANLGSRVLVELQLRLQHTV*LGQIISPA PFLPALKRSSVGGAAYPLSLGWLLH*GESRLRGT EVLPVFDLVGGTKSP**YP*PHPYWVHKTQVTL GGQGVLVAGEESVDGWLRTVQKGVAGPSFFAWK FKF*GTQSLQH*MGAGGSTCLPSWKGRVCAECYR WKATGGRSRSRGDLPLPNRAAQGDGRHPTFLRA *PSDARALAADSLGAPGDLPANRSKSFPRGSGR CTLFVEYDCSPRTASSL*VALLPDFSPGFKPWVA AYYQNRSVAPSLPAEGWHH*MS*AAEQRPCRGT PRPETTTPVPLRTFIRAPSTWPAWLSNHTPVST TGTQQPREVWRGPGDAWLRQEQASSQPRCSHSI* EA*APPSPSAAVTSLTAWRGRPDGLHPLWPTPA *VTTEASSGP*RPQAGLQWKAWAGPIGALPHQG CLRSPTTWHTPSAGPSTAWKM*PRSASRAIRPPP WPSWAPRAWLPPLTACPPSTRIPPAVGRGGSSS PNQSEGSKPDTRGRGSWGRWESQAGAALGILPQ TGARALWSWPIWPGPGAQRLRSGAATMEACRRWR HCQGLSTVGLCQVSRRWKLLASMAAGSGVETTS GTRAAAPRTQVLRSEREMAQPDPTGHCHPAAFP VGLVRGQPGHCLAGMGGSLQVAPVALAAVLGSIL PSRDLAEAPGVWGVGWKHWLPGRDNKGFGSF*

Frame 2

RGGVEGRAHAHRTTRPDRRRERRPSSLWEL*FC RRGSRGARPAEHSVSQRAERAARRCGADQDGDC RAALSR*VCGEGGRAARAGRASGRSPSAALIPE LEGPAVLADPRTSRPGPWDPQHPCEARRPVQPDW TVPRGTESQLGTESSFVLWRGGLAGTYSGERPG SAFMWRKRRLPSAYGHEGVNSTFHSLPAFAENP EKRTTGVPILQPDPRYPSECPADQAASGRGGVPL QRTGVRIWEAGFWLNSSFVCNILCDLGKLFPPP RSCQL*NGHQWGVLRIPFHWGGFFTEESRASEE LRSCLCSTWWGALRAPDSTPDPILIGCTRHRSLW AGKEFW*QERSRWMDG*GQCRRVWLGRLFLPGN SSSEAPSHSSTKWVQEAALVCPAGKAGYVLSVT GGRPLEVAPGAAGIYLCLTGLLKVMVDTPLS*EL DPQMPGPWLQIPWELPGIFQQIGANLFPVDQEG ARSLWNTTAHPAQQAAYKWPSCLISALGSSPGW LLTTKIAQ*LQACLQRVGTIK*VEQLNRGHAGAL RGLRRPRLCR*GPSSGLRPLGPLGCPITLQCQP LAPSSQERCGVALGTHG*GRNRPPPSHGAAIPS ERHELHLLPAQP*HL*LPGGGGQTGSILCGPHQH E*QRRLQAAPSALRPVSSGRPGQGPSEPCLTKG ASGPRLRGTPRALDQVQPGRCDRGQRAEQSGHR PGLPGLPEPGCPH*LRVLLQPGSLQLWGGEGHLH QTSPRGRSQTREEEGPGEGGRARQGRPWESCHR QGRGPCGAGPSGRAREPRG*GVGQPPWRPAGGG GTVRVCPQWVCARSPAGGNCWLPWQQEAESRPLP EQEQQPRGPRC*GLRGRWPSLTPLATAILLPSQ WGWSGGSLATA*LEWEEACRWHRWPWLQFWAAS SQAETLLKLLGCGVWAGSTGSLVGTIKVLGLSE

Sequence TSSC4.dAug05

TSSC4.F10 (forward primer)
TSSC4.R7 (reverse primer)
Intron retention

Frame 0

PSSSCSWSIPVLFPQQMPSFPGLLPSRTQGPGR GGVEGRAHAHRTTRPDRRRERRPSSLWEL*FCRR GSRGARPAEHSVSQRAERAARRCGADQDGDCRA ALSRKVHALCGIRLLTPHSKQLISGPPA*FQPW VQALGGCLLPKSLSSSKPACRGLAPLNELSS*TE AMPGHSEA*DDHACAAEDLHQGSVHLARLAVQS HSSVNHWHPAAKRGVAWPWGRMAEAGTGLLPAT VQPFHLRGMSSTFSQRSRDIFDCLEGAARRAPSS VAHTSMSDNGGFKRPLAPSGRSPVEGLGRAHRS PASPRVPPVPDYVAHPERWTKYSLEDVTEVSRR WKLLASMAAGSGVETTSGTRAAAPRTQVLRSERE MAQPDPTGHCHPAAFPVGLVRGQPGHCLAGMGG SLQVAPVALAAVLGSILPSRDLAEAPGVWGVGW KHWLPGRDNKGFGSF*

Frame 1

PPLPVLGQFRSCFPNKCRRFRGCFRAGPKGRGV EE*RGERMRTGLHVPTGVGSGGPVPCGSCSSAG AEAVVLGRQSTRFPRGLSAPHGGAAPTKMETAEQ P*AGRCTLFVEYDCSPRTASSL*VALLPDFSPG FKPWVAAYYQNRSVAPSLPAEGWHH*MS*AAEQ RPCRGTPRPETTTPVPLRTFIRAPSTWPAWLSNH TPVSTTGTQQPREVWRGPGDAWLRQEQASSQPR CSHSI*EA*APPSPSAAVTSLTAWRGRPDGLHP LWPTPA*VTTEASSGP*RPQAGLQWKAWAGPIGA LPHQGCLRSPTTWHTPSAGPSTAWKM*PRSPAG GNCWLPWQQEAESRPLPEQEQQPRGPRC*GLRG RWPSLTPLATAILLPSQWGWSGGSLATA*LEWEE ACRWHRWPWLQFWAASSQAETLLKLLGCGVWAG STGSLVGTIKVLGLSE

Frame 2

LLFLFLVNSGLVSPTNAVVSGAASEPDPRAGAW RSRGASACAQDYTSRQASGAAAQFLVGAVVLQA RKPWCSAGRALGFPEG*ARRTEVRRRPRWRLPS SLEPEGARSLWNTTAHPAQQAAYKWPSCLISALG SSPGWLLTTKIAQ*LQACLQRVGTIK*VEQLNR GHAGALRGLRRPRLCR*GPSSGLRPLGPLGCPI TLQCQPLAPSSQERCGVALGTHG*GRNRPPPSHG AAIPSERHELHLLPAQP*HL*LPGGGGQTGSIL CGPHQHE*QRRLQAAPSALRPVSSGRPGQGPSE PCLTKGASGPRLRGTPRALDQVQPGRCDRGLPPV ETVGFHGSRKRSRDHFRNKSSSPEDPGAEV*EG DGPA*PHWPLPSCCLPSGAGQGAAWPLPSWNGR KPAGGTGGPGCSSGQHPPKQRPC*SSWGVGCGLE ALAPW*GQ*RFWVFLR

Sequence TSSC4.cAug05

CCCTCCTCTTCCTGTTCTTGGTCAATTCCGGTCTTGTTTCCCCAACAAATGCCGTCGTTTCCGGGGCTGCTTCCGAGCCGGACCCAAGGGCCGGGGCGTG GAGGAGTAGAGGGGCGAGCGCATGCGCACAGGACTACACGTCCCGACAGGCGTCGGGAGCGGCGGCCCAGTTCCTTGTGGGAGCTGTAGTTCTGCAGGCG CGGAAGCCGTGGTGCTCGGCCGGCAGAGCACTCGGTTTCCCAGAGGGCTGAGCGCGCCGCACGGAGGTGCGGCGCCGACCAAGATGGAGACTGCCGAGCA GCCTTGAGCCGTTGAGCAGCTGAACAGAGGCCATGCCGGGGCACTCCGAGGCCTGAGACGACCACGCCTGTGCCGCTGAGGACCTTCATCAGGGCTCCGT CCACTTGGCCCGCTTGGCTGTCCAATCACACTCCAGTGTCAACCACTGGCACCCAGCAGCCAAGAGAGGTGTGGCGTGGCCCTGGGGACGCATGGCTGAG GCAGGAACAGGCCTCCTCCCAGCCACGGTGCAGCCATTCCATCTGAGAGGCATGAGCTCCACCTTCTCCCAGCGCAGCCGTGACATCTTTGACTGCCTGG AGGGGGCGGCCAGACGGGCTCCATCCTCTGTGGCCCACACCAGCATGAGTGACAACGGAGGCTTCAAGCGGCCCCTAGCGCCCTCAGGCCGGTCTCCAGT GGAAGGCCTGGGCAGGGCCCATCGGAGCCCTGCCTCACCAAGGGTGCCTCCGGTCCCCGACTACGTGGCACACCCCGAGCGCTGGACCAAGTACAGCCTG GAAGATGTGACCGAGGTCAGCGAGCAGAGCAATCAGGCCACCGCCCTGGCCTTCCTGGGCTCCCAGAGCCTGGCTGCCCCCACTGACTGCGTGTCCTCCT TCAACCAGGATCCCTCCAGCTGTGGGGAGGGGAGGGTCATCTTCACCAAACCAGTCCGAGGGGTCGAAGCCAGACACGAGAGGAAGAGGGTCCTGGGGAA GGTGGGAGAGCCAGGCAGGGGCGGCCTTGGGAATCCTGCCACAGACAGGGGCGAGGGCCCTGTGGAGCTGGCCCATCTGGCCGGGCCCGGGAGCCCAGAG GCTGAGGAGTGGGGCAGCCACCATGGAGGCCTGCAGGAGGTGGAGGCACTGTCAGGGTCTGTCCACAGTGGGTCTGTGCCAGGTCTCCCGCCGGTGGAAA CTGTTGGCTTCCATGGCAGCAGGAAGCGGAGTCGAGACCACTTCCGGAACAAGAGCAGCAGCCCCGAGGACCCAGGTGCTGAGGTCTGAGAGGGAGATGG CCCAGCCTGACCCCACTGGCCACTGCCATCCTGCTGCCTTCCCAGTGGGGCTGGTCAGGGGGCAGCCTGGCCACTGCCTAGCTGGAATGGGAGGAAGCCT GCAGGTGGCACCGGTGGCCCTGGCTGCAGTTCTGGGCAGCATCCTCCCAAGCAGAGACCTTGCTGAAGCTCCTGGGGTGTGGGGTGTGGGCTGGAAGCAC TGGCTCCCTGGTAGGGACAATAAAGGTTTTGGGTCTTTCTGAGA

TSSC4.F10 (forward primer)
TSSC4.R7 (reverse primer)
Intron retention

Frame 0

PSSSCSWSIPVLFPQQMPSFPGLLPSRTQGPGR GGVEGRAHAHRTTRPDRRRERRPSSLWEL*FCRR GSRGARPAEHSVSQRAERAARRCGADQDGDCRA ALSR*AAEQRPCRGTPRPETTTPVPLRTFIRAP STWPAWLSNHTPVSTTGTQQPREVWRGPGDAWLR QEQASSQPRCSHSI*EA*APPSPSAAVTSLTAW RGRPDGLHPLWPTPA*VTTEASSGP*RPQAGLQ WKAWAGPIGALPHQGCLRSPTTWHTPSAGPSTAW KM*PRSASRAIRPPPWPSWAPRAWLPPLTACPP STRIPPAVGRGGSSSPNQSEGSKPDTRGRGSWG RWESQAGAALGILPQTGARALWSWPIWPGPGAQR LRSGAATMEACRRWRHCQGLSTVGLCQVSRRWK LLASMAAGSGVETTSGTRAAAPRTQVLRSEREM AQPDPTGHCHPAAFPVGLVRGQPGHCLAGMGGSL QVAPVALAAVLGSILPSRDLAEAPGVWGVGWKH WLPGRDNKGFGSF*

Frame 1

PPLPVLGQFRSCFPNKCRRFRGCFRAGPKGRGV EE*RGERMRTGLHVPTGVGSGGPVPCGSCSSAG AEAVVLGRQSTRFPRGLSAPHGGAAPTKMETAEQ P*AVEQLNRGHAGALRGLRRPRLCR*GPSSGLR PLGPLGCPITLQCQPLAPSSQERCGVALGTHG* GRNRPPPSHGAAIPSERHELHLLPAQP*HL*LPG GGGQTGSILCGPHQHE*QRRLQAAPSALRPVSS GRPGQGPSEPCLTKGASGPRLRGTPRALDQVQP GRCDRGQRAEQSGHRPGLPGLPEPGCPH*LRVLL QPGSLQLWGGEGHLHQTSPRGRSQTREEEGPGE GGRARQGRPWESCHRQGRGPCGAGPSGRAREPR G*GVGQPPWRPAGGGGTVRVCPQWVCARSPAGGN CWLPWQQEAESRPLPEQEQQPRGPRC*GLRGRW PSLTPLATAILLPSQWGWSGGSLATA*LEWEEA CRWHRWPWLQFWAASSQAETLLKLLGCGVWAGST GSLVGTIKVLGLSE

Frame 2

LLFLFLVNSGLVSPTNAVVSGAASEPDPRAGAW RSRGASACAQDYTSRQASGAAAQFLVGAVVLQA RKPWCSAGRALGFPEG*ARRTEVRRRPRWRLPS SLEPLSS*TEAMPGHSEA*DDHACAAEDLHQGSV HLARLAVQSHSSVNHWHPAAKRGVAWPWGRMAE AGTGLLPATVQPFHLRGMSSTFSQRSRDIFDCL EGAARRAPSSVAHTSMSDNGGFKRPLAPSGRSPV EGLGRAHRSPASPRVPPVPDYVAHPERWTKYSL EDVTEVSEQSNQATALAFLGSQSLAAPTDCVSS FNQDPSSCGEGRVIFTKPVRGVEARHERKRVLGK VGEPGRGGLGNPATDRGEGPVELAHLAGPGSPE AEEWGSHHGGLQEVEALSGSVHSGSVPGLPPVE TVGFHGSRKRSRDHFRNKSSSPEDPGAEV*EGDG PA*PHWPLPSCCLPSGAGQGAAWPLPSWNGRKP AGGTGGPGCSSGQHPPKQRPC*SSWGVGCGLEA LAPW*GQ*RFWVFLR

Sequence TSSC4.hAug05

GGCGTGGAGGAGTAGAGGGGCGAGCGCATGCGCACAGGACTACACGTCCCGACAGGCGTCGGGAGCGGCGGCCCAGTTCCTTGTGGGAGCTGTAGTTCTG CAGGCGCGGAAGCCGTGGTGCTCGGCCGGCAGAGCACTCGGTTTCCCAGAGGGCTGAGCGCGCCGCACGGAGGTGCGGCGCCGACCAAGATGGAGACTGC CGAGCAGCCTTGAGCCGGTAGGTTTGTGGTGAGGGAGGACGGGCCGCGCGGGCCGGCCGAGCCTCCGGGAGGTCACCGAGCGCAGCTTTAATACCTGAGC TCGAAGGCCCCGCTGTGCTCGCCGACCCCCGTACCTCGCGGCCGGGCCCTTGGGACCCACAGCATCCTTGTGAGGCCCGGAGGCCTGTCCAGCCCGACTG GACAGTGCCGAGGGGCACCGAGAGCCAGCTTGGCACCGAGAGTTCGTTTGTTCTCTGGCGGGGAGGTCTTGCTGGCACATATAGTGGAGAAAGGCCGGGC TCTGCGTTCATGTGGAGAAAGAGACGGCTTCCTTCAGCCTACGGACATGAAGGAGTCAACTCTACCTTCCACTCGTTGCCGGCTTTCGCCGAGAACCCCG AGAAACGGACTACCGGAGTCCCTATCTTGCAGCCCGATCCCCGCTACCCGTCGGAGTGCCCCGCTGACCAGGCTGCTTCTGGCCGCGGCGGCGTTCCGCT GCAGAGGACGGGAGTGCGAATCTGGGAAGCAGGGTTCTGGTTGAACTCCAGCTTCGTCTGCAACATACTGTGTGACTTGGGCAAATTATTTCCCCCGCCC CGTTCCTGCCAGCTTTAAAACGGTCATCAGTGGGGGGTGCTGCGTATCCCCTTTCACTGGGGTGGCTTCTTCACTGAGGAGAGTCGCGCCTCAGAGGAAC TGAGTTGAGCAGCTGAACAGAGGCCATGCCGGGGCACTCCGAGGCCTGAGACGACCACGCCTGTGCCGCTGAGGACCTTCATCAGGGCTCCGTCCACTTG GCCCGCTTGGCTGTCCAATCACACTCCAGTGTCAACCACTGGCACCCAGCAGCCAAGAGAGGTGTGGCGTGGCCCTGGGGACGCATGGCTGAGGCAGGAA CAGGTGAGCCGTCCCCCAGCGTGGAGGGCGAACACGGGACGGAGTATGACACGCTGCCTTCCGACACAGTCTCCCTCAGTGACTCGGACTCTGACCTCAG CTTGCCCGGTGGTGCTGAAGTGGAAGCACTGTCCCCGATGGGGCTGCCTGGGGAGGAGGATTCAGGTCCTGATGAGCCGCCCTCACCCCCGTCAGGCCTC CTCCCAGCCACGGTGCAGCCATTCCATCTGAGAGGCATGAGCTCCACCTTCTCCCAGCGCAGCCGTGACATCTTTGACTGCCTGGAGGGGGCGGCCAGAC GGGCTCCATCCTCTGTGGCCCACACCAGCATGAGTGACAACGGAGGCTTCAAGCGGCCCCTAGCGCCCTCAGGCCGGTCTCCAGTGGAAGGCCTGGGCAG GGCCCATCGGAGCCCTGCCTCACCAAGGGTGCCTCCGGTCCCCGACTACGTGGCACACCCCGAGCGCTGGACCAAGTACAGCCTGGAAGATGTGACCGAG GTCAGCGAGCAGAGCAATCAGGCCACCGCCCTGGCCTTCCTGGGCTCCCAGAGCCTGGCTGCCCCCACTGACTGCGTGTCCTCCTTCAACCAGGATCCCT CCAGCTGTGGGGAGGGGAGGGTCATCTTCACCAAACCAGTCCGAGGGGTCGAAGCCAGACACGAGAGGAAGAGGGTCCTGGGGAAGGTGGGAGAGCCAGG CAGGGGCGGCCTTGGGAATCCTGCCACAGACAGGGGCGAGGGCCCTGTGGAGCTGGCCCATCTGGCCGGGCCCGGGAGCCCAGAGGCTGAGGAGTGGGGC AGCCACCATGGAGGCCTGCAGGAGGTGGAGGCACTGTCAGGGTCTGTCCACAGTGGGTCTGTGCCAGGTCTCCCGCCGGTGGAAACTGTTGGCTTCCATG GCAGCAGGAAGCGGAGTCGAGACCACTTCCGGAACAAGAGCAGCAGCCCCGAGGACCCAGGTGCTGAGGTCTGAGAGGGAGATGGCCCAGCCTGACCCCA CTGGCCACTGCCATCCTGCTGCCTTCCCAGTGGGGCTGGTCAGGGGGCAGCCTGGCCACTGCCTAGCTGGAATGGGAGGAAGCCTGCAGGTGGCACCGGT GGCCCTGGCTGCAGTTCTGGGCAGCATCCTCCCAAGCAGAGACCTTGCTGAAGCTCCTGGGGTGTGGGGTGTGGGCTGGAAGCACTGGCTCCCTGGTAGG GACAATAAAGGTTTTGGGTCTTTCTGAGA

TSSC4.F10 (forward primer)
TSSC4.R7 (reverse primer)
Intron retention

Frame 0

GVEE*RGERMRTGLHVPTGVGSGGPVPCGSCSS AGAEAVVLGRQSTRFPRGLSAPHGGAAPTKMETA EQP*AGRFVVREDGPRGPAEPPGGHRAQL*YLS SKAPLCSPTPVPRGRALGTHSILVRPGGLSSPT GQCRGAPRASLAPRVRLFSGGEVLLAHIVEKGRA LRSCGERDGFLQPTDMKESTLPSTRCRLSPRTP RNGLPESLSCSPIPATRRSAPLTRLLLAAAAFR CRGRECESGKQGSG*TPASSATYCVTWANYFPRP VPASFKTVISGGCCVSPFTGVASSLRRVAPQRN *VEQLNRGHAGALRGLRRPRLCR*GPSSGLRPL GPLGCPITLQCQPLAPSSQERCGVALGTHG*GRN R*AVPQRGGRTRDGV*HAAFRHSLPQ*LGL*PQ LARWC*SGSTVPDGAAWGGGFRS**AALTPVRP PPSHGAAIPSERHELHLLPAQP*HL*LPGGGGQT GSILCGPHQHE*QRRLQAAPSALRPVSSGRPGQ GPSEPCLTKGASGPRLRGTPRALDQVQPGRCDR GQRAEQSGHRPGLPGLPEPGCPH*LRVLLQPGSL QLWGGEGHLHQTSPRGRSQTREEEGPGEGGRAR QGRPWESCHRQGRGPCGAGPSGRAREPRG*GVG QPPWRPAGGGGTVRVCPQWVCARSPAGGNCWLPW QQEAESRPLPEQEQQPRGPRC*GLRGRWPSLTP LATAILLPSQWGWSGGSLATA*LEWEEACRWHR WPWLQFWAASSQAETLLKLLGCGVWAGSTGSLVG TIKVLGLSE

Frame 1

AWRSRGASACAQDYTSRQASGAAAQFLVGAVVL QARKPWCSAGRALGFPEG*ARRTEVRRRPRWRL PSSLEPVGLW*GRTGRAGRPSLREVTERSFNT*A RRPRCARRPPYLAAGPLGPTASL*GPEACPARL DSAEGHREPAWHREFVCSLAGRSCWHI*WRKAG LCVHVEKETASFSLRT*RSQLYLPLVAGFRREPR ETDYRSPYLAARSPLPVGVPR*PGCFWPRRRSA AEDGSANLGSRVLVELQLRLQHTV*LGQIISPA PFLPALKRSSVGGAAYPLSLGWLLH*GESRLRGT ELSS*TEAMPGHSEA*DDHACAAEDLHQGSVHL ARLAVQSHSSVNHWHPAAKRGVAWPWGRMAEAG TGEPSPSVEGEHGTEYDTLPSDTVSLSDSDSDLS LPGGAEVEALSPMGLPGEEDSGPDEPPSPPSGL LPATVQPFHLRGMSSTFSQRSRDIFDCLEGAAR RAPSSVAHTSMSDNGGFKRPLAPSGRSPVEGLGR AHRSPASPRVPPVPDYVAHPERWTKYSLEDVTE VSEQSNQATALAFLGSQSLAAPTDCVSSFNQDP SSCGEGRVIFTKPVRGVEARHERKRVLGKVGEPG RGGLGNPATDRGEGPVELAHLAGPGSPEAEEWG SHHGGLQEVEALSGSVHSGSVPGLPPVETVGFH GSRKRSRDHFRNKSSSPEDPGAEV*EGDGPA*PH WPLPSCCLPSGAGQGAAWPLPSWNGRKPAGGTG GPGCSSGQHPPKQRPC*SSWGVGCGLEALAPW* GQ*RFWVFLR

Frame 2

RGGVEGRAHAHRTTRPDRRRERRPSSLWEL*FC RRGSRGARPAEHSVSQRAERAARRCGADQDGDC RAALSR*VCGEGGRAARAGRASGRSPSAALIPE LEGPAVLADPRTSRPGPWDPQHPCEARRPVQPDW TVPRGTESQLGTESSFVLWRGGLAGTYSGERPG SAFMWRKRRLPSAYGHEGVNSTFHSLPAFAENP EKRTTGVPILQPDPRYPSECPADQAASGRGGVPL QRTGVRIWEAGFWLNSSFVCNILCDLGKLFPPP RSCQL*NGHQWGVLRIPFHWGGFFTEESRASEE LS*AAEQRPCRGTPRPETTTPVPLRTFIRAPSTW PAWLSNHTPVSTTGTQQPREVWRGPGDAWLRQE QVSRPPAWRANTGRSMTRCLPTQSPSVTRTLTS ACPVVLKWKHCPRWGCLGRRIQVLMSRPHPRQAS SQPRCSHSI*EA*APPSPSAAVTSLTAWRGRPD GLHPLWPTPA*VTTEASSGP*RPQAGLQWKAWA GPIGALPHQGCLRSPTTWHTPSAGPSTAWKM*PR SASRAIRPPPWPSWAPRAWLPPLTACPPSTRIP PAVGRGGSSSPNQSEGSKPDTRGRGSWGRWESQ AGAALGILPQTGARALWSWPIWPGPGAQRLRSGA ATMEACRRWRHCQGLSTVGLCQVSRRWKLLASM AAGSGVETTSGTRAAAPRTQVLRSEREMAQPDP TGHCHPAAFPVGLVRGQPGHCLAGMGGSLQVAPV ALAAVLGSILPSRDLAEAPGVWGVGWKHWLPGR DNKGFGSF*

Sequence TSSC4.gAug05

CCCTCCTCTTCCTGTTCTTGGTCAATTCCGGTCTTGTTTCCCCAACAAATGCCGTCGTTTCCGGGGCTGCTTCCGAGCCGGACCCAAGGGCCGGGGCGTG GAGGAGTAGAGGGGCGAGCGCATGCGCACAGGACTACACGTCCCGACAGGCGTCGGGAGCGGCGGCCCAGTTCCTTGTGGGAGCTGTAGTTCTGCAGGCG CGGAAGCCGTGGTGCTCGGCCGGCAGAGCACTCGGTTTCCCAGAGGGCTGAGCGCGCCGCACGGAGGTGCGGCGCCGACCAAGATGGAGACTGCCGAGCA GCCTTGAGCCGGGCTGCTCAAGGTGATGGTCGACACCCCACTTTCCTGAGAGCTTGACCCTCAGATGCCAGGGCCTTGGCTGCAGATTCCTTGGGAGCTC CCGGGGATCTTCCAGCAAATAGGAGCAAATCTTTTCCCCGTGGATCAGGAAGGTGCACGCTCTTTGTGGAATACGACTGCTCACCCCGCACAGCAAGCAG CTTATAAGTGGCCCTCCTGCCTGATTTCAGCCCTGGGTTCAAGCCCTGGGTGGCTGCTTACTACCAAAATCGCTCAGTAGCTCCAAGCCTGCCTGCAGAG GGTTGGCACCATTAAATGAGTTGAGCAGCTGAACAGAGGCCATGCCGGGGCACTCCGAGGCCTGAGACGACCACGCCTGTGCCGCTGAGGACCTTCATCA GGGCTCCGTCCACTTGGCCCGCTTGGCTGTCCAATCACACTCCAGTGTCAACCACTGGCACCCAGCAGCCAAGAGAGGTGTGGCGTGGCCCTGGGGACGC ATGGCTGAGGCAGGAACAGGTGAGCCGTCCCCCAGCGTGGAGGGCGAACACGGGACGGAGTATGACACGCTGCCTTCCGACACAGTCTCCCTCAGTGACT CGGACTCTGACCTCAGCTTGCCCGGTGGTGCTGAAGTGGAAGCACTGTCCCCGATGGGGCTGCCTGGGGAGGAGGATTCAGGTCCTGATGAGCCGCCCTC ACCCCCGTCAGGCCTCCTCCCAGCCACGGTGCAGCCATTCCATCTGAGAGGCATGAGCTCCACCTTCTCCCAGCGCAGCCGTGACATCTTTGACTGCCTG GAGGGGGCGGCCAGACGGGCTCCATCCTCTGTGGCCCACACCAGCATGAGTGACAACGGAGGCTTCAAGCGGCCCCTAGCGCCCTCAGGCCGGTCTCCAG TGGAAGGCCTGGGCAGGGCCCATCGGAGCCCTGCCTCACCAAGGGTGCCTCCGGTCCCCGACTACGTGGCACACCCCGAGCGCTGGACCAAGTACAGCCT GGAAGATGTGACCGAGGTCAGCGAGCAGAGCAATCAGGCCACCGCCCTGGCCTTCCTGGGCTCCCAGAGCCTGGCTGCCCCCACTGACTGCGTGTCCTCC TTCAACCAGGATCCCTCCAGCTGTGGGGAGGGGAGGGTCATCTTCACCAAACCAGTCCGAGGGGTCGAAGCCAGACACGAGAGGAAGAGGGTCCTGGGGA AGGTGGGAGAGCCAGGCAGGGGCGGCCTTGGGAATCCTGCCACAGACAGGGGCGAGGGCCCTGTGGAGCTGGCCCATCTGGCCGGGCCCGGGAGCCCAGA GGCTGAGGAGTGGGGCAGCCACCATGGAGGCCTGCAGGAGGTGGAGGCACTGTCAGGGTCTGTCCACAGTGGGTCTGTGCCAGGTCTCCCGCCGGTGGAA ACTGTTGGCTTCCATGGCAGCAGGAAGCGGAGTCGAGACCACTTCCGGAACAAGAGCAGCAGCCCCGAGGACCCAGGTGCTGAGGTCTGAGAGGGAGATG GCCCAGCCTGACCCCACTGGCCACTGCCATCCTGCTGCCTTCCCAGTGGGGCTGGTCAGGGGGCAGCCTGGCCACTGCCTAGCTGGAATGGGAGGAAGCC TGCAGGTGGCACCGGTGGCCCTGGCTGCAGTTCTGGGCAGCATCCTCCCAAGCAGAGACCTTGCTGAAGCTCCTGGGGTGTGGGGTGTGGGCTGGAAGCA CTGGCTCCCTGGTAGGGACAATAAAGGTTTTGGGTCTTTCTGAGA

TSSC4.F10 (forward primer)
TSSC4.R7 (reverse primer)
Intron retention

Frame 0

PSSSCSWSIPVLFPQQMPSFPGLLPSRTQGPGR GGVEGRAHAHRTTRPDRRRERRPSSLWEL*FCRR GSRGARPAEHSVSQRAERAARRCGADQDGDCRA ALSRAAQGDGRHPTFLRA*PSDARALAADSLGA PGDLPANRSKSFPRGSGRCTLFVEYDCSPRTASS L*VALLPDFSPGFKPWVAAYYQNRSVAPSLPAE GWHH*MS*AAEQRPCRGTPRPETTTPVPLRTFI RAPSTWPAWLSNHTPVSTTGTQQPREVWRGPGDA WLRQEQVSRPPAWRANTGRSMTRCLPTQSPSVT RTLTSACPVVLKWKHCPRWGCLGRRIQVLMSRP HPRQASSQPRCSHSI*EA*APPSPSAAVTSLTAW RGRPDGLHPLWPTPA*VTTEASSGP*RPQAGLQ WKAWAGPIGALPHQGCLRSPTTWHTPSAGPSTA WKM*PRSASRAIRPPPWPSWAPRAWLPPLTACPP STRIPPAVGRGGSSSPNQSEGSKPDTRGRGSWG RWESQAGAALGILPQTGARALWSWPIWPGPGAQ RLRSGAATMEACRRWRHCQGLSTVGLCQVSRRWK LLASMAAGSGVETTSGTRAAAPRTQVLRSEREM AQPDPTGHCHPAAFPVGLVRGQPGHCLAGMGGS LQVAPVALAAVLGSILPSRDLAEAPGVWGVGWKH WLPGRDNKGFGSF*

Frame 1

PPLPVLGQFRSCFPNKCRRFRGCFRAGPKGRGV EE*RGERMRTGLHVPTGVGSGGPVPCGSCSSAG AEAVVLGRQSTRFPRGLSAPHGGAAPTKMETAEQ P*AGLLKVMVDTPLS*ELDPQMPGPWLQIPWEL PGIFQQIGANLFPVDQEGARSLWNTTAHPAQQA AYKWPSCLISALGSSPGWLLTTKIAQ*LQACLQR VGTIK*VEQLNRGHAGALRGLRRPRLCR*GPSS GLRPLGPLGCPITLQCQPLAPSSQERCGVALGT HG*GRNR*AVPQRGGRTRDGV*HAAFRHSLPQ*L GL*PQLARWC*SGSTVPDGAAWGGGFRS**AAL TPVRPPPSHGAAIPSERHELHLLPAQP*HL*LP GGGGQTGSILCGPHQHE*QRRLQAAPSALRPVSS GRPGQGPSEPCLTKGASGPRLRGTPRALDQVQP GRCDRGQRAEQSGHRPGLPGLPEPGCPH*LRVL LQPGSLQLWGGEGHLHQTSPRGRSQTREEEGPGE GGRARQGRPWESCHRQGRGPCGAGPSGRAREPR G*GVGQPPWRPAGGGGTVRVCPQWVCARSPAGG NCWLPWQQEAESRPLPEQEQQPRGPRC*GLRGRW PSLTPLATAILLPSQWGWSGGSLATA*LEWEEA CRWHRWPWLQFWAASSQAETLLKLLGCGVWAGS TGSLVGTIKVLGLSE

Frame 2

LLFLFLVNSGLVSPTNAVVSGAASEPDPRAGAW RSRGASACAQDYTSRQASGAAAQFLVGAVVLQA RKPWCSAGRALGFPEG*ARRTEVRRRPRWRLPS SLEPGCSR*WSTPHFPESLTLRCQGLGCRFLGSS RGSSSK*EQIFSPWIRKVHALCGIRLLTPHSKQ LISGPPA*FQPWVQALGGCLLPKSLSSSKPACR GLAPLNELSS*TEAMPGHSEA*DDHACAAEDLHQ GSVHLARLAVQSHSSVNHWHPAAKRGVAWPWGR MAEAGTGEPSPSVEGEHGTEYDTLPSDTVSLSD SDSDLSLPGGAEVEALSPMGLPGEEDSGPDEPPS PPSGLLPATVQPFHLRGMSSTFSQRSRDIFDCL EGAARRAPSSVAHTSMSDNGGFKRPLAPSGRSP VEGLGRAHRSPASPRVPPVPDYVAHPERWTKYSL EDVTEVSEQSNQATALAFLGSQSLAAPTDCVSS FNQDPSSCGEGRVIFTKPVRGVEARHERKRVLG KVGEPGRGGLGNPATDRGEGPVELAHLAGPGSPE AEEWGSHHGGLQEVEALSGSVHSGSVPGLPPVE TVGFHGSRKRSRDHFRNKSSSPEDPGAEV*EGD GPA*PHWPLPSCCLPSGAGQGAAWPLPSWNGRKP AGGTGGPGCSSGQHPPKQRPC*SSWGVGCGLEA LAPW*GQ*RFWVFLR

Sequence TSSC4.eAug05

GGCGTGGAGGAGTAGAGGGGCGAGCGCATGCGCACAGGACTACACGTCCCGACAGGCGTCGGGAGCGGCGGCCCAGTTCCTTGTGGGAGCTGTAGTTCTG CAGGCGCGGAAGCCGTGGTGCTCGGCCGGCAGAGCACTCGGTTTCCCAGAGGGCTGAGCGCGCCGCACGGAGGTGCGGCGCCGACCAAGATGGAGACTGC CGAGCAGCCTTGAGCCGGTAGGTTTGTGGTGAGGGAGGACGGGCCGCGCGGGCCGGCCGAGCCTCCGGGAGGTCACCGAGCGCAGCTTTAATACCTGAGC TCGAAGGCCCCGCTGTGCTCGCCGACCCCCGTACCTCGCGGCCGGGCCCTTGGGACCCACAGCATCCTTGTGAGGCCCGGAGGCCTGTCCAGCCCGACTG GACAGTGCCGAGGGGCACCGAGAGCCAGCTTGGCACCGAGAGTTCGTTTGTTCTCTGGCGGGGAGGTCTTGCTGGCACATATAGTGGAGAAAGGCCGGGC TCTGCGTTCATGTGGAGAAAGAGACGGCTTCCTTCAGCCTACGGACATGAAGGAGTCAACTCTACCTTCCACTCGTTGCCGGCTTTCGCCGAGAACCCCG AGAAACGGACTACCGGAGTCCCTATCTTGCAGCCCGATCCCCGCTACCCGTCGGAGTGCCCCGCTGACCAGGCTGCTTCTGGCCGCGGCGGCGTTCCGCT GCAGAGGACGGGAGTGCGAATCTGGGAAGCAGGGTTCTGGTTGAACTCCAGCTTCGTCTGCAACATACTGTGTGACTTGGGCAAATTATTTCCCCCGCCC CGTTCCTGCCAGCTTTAAAACGGTCATCAGTGGGGGGTGCTGCGTATCCCCTTTCACTGGGGTGGCTTCTTCACTGAGGAGAGTCGCGCCTCAGAGGAAC TGAGGTCCTGCCTGTGTTCGACCTGGTGGGGGGCACTAAGAGCCCCTGATAGTACCCCTGACCCCATCCTTATTGGGTGCACAAGACACAGGTCACTCTG GGCGGGCAAGGAGTTTTGGTAGCAGGAGAGGAGTCGGTGGATGGATGGCTGAGGACAGTGCAGAAGGGTGTGGCTGGGCCGTCTTTTTTTGCCTGGAAAT TCAAGTTCTGAGGCACCCAGTCACTCCAGCACTAAATGGGTGCAGGAGGCAGCACTTGTCTGCCCAGCTGGAAAGGCAGGGTATGTGCTGAGTGTTACAG GTGGAAGGCCACTGGAGGTCGCTCCAGGAGCCGCGGGGATTTACCTCTGCCTAACAGGGCTGCTCAAGGTGATGGTCGACACCCCACTTTCCTGAGAGCT TGACCCTCAGATGCCAGGGCCTTGGCTGCAGATTCCTTGGGAGCTCCCGGGGATCTTCCAGCAAATAGGAGCAAATCTTTTCCCCGTGGATCAGGAAGGT GCACGCTCTTTGTGGAATACGACTGCTCACCCCGCACAGCAAGCAGCTTATAAGTGGCCCTCCTGCCTGATTTCAGCCCTGGGTTCAAGCCCTGGGTGGC TGCTTACTACCAAAATCGCTCAGTAGCTCCAAGCCTGCCTGCAGAGGGTTGGCACCATTAAATGAGGTAACGAGTCAAAAGTCCCTACCCTGGGTCCTAG CCTGTCAGGGGCTCCGAAAACCCAGGCTCAGGTCGGTCCTGCCCGGCACCTGTTTCACACATGTACACTCCGGTCTGAGGTTGGTCCTCTCCCCCACCCC ACCCACCTGCAGTTGAGCAGCTGAACAGAGGCCATGCCGGGGCACTCCGAGGCCTGAGACGACCACGCCTGTGCCGCTGAGGACCTTCATCAGGGCTCCG TCCACTTGGCCCGCTTGGCTGTCCAATCACACTCCAGTGTCAACCACTGGCACCCAGCAGCCAAGAGAGGTGTGGCGTGGCCCTGGGGACGCATGGCTGA GGCAGGAACAGGTGAGCCGTCCCCCAGCGTGGAGGGCGAACACGGGACGGAGTATGACACGCTGCCTTCCGACACAGTCTCCCTCAGTGACTCGGACTCT GACCTCAGCTTGCCCGGTGGTGCTGAAGTGGAAGCACTGTCCCCGATGGGGCTGCCTGGGGAGGAGGATTCAGGTCCTGATGAGCCGCCCTCACCCCCGT CAGGCCTCCTCCCAGCCACGGTGCAGCCATTCCATCTGAGAGGCATGAGCTCCACCTTCTCCCAGCGCAGCCGTGACATCTTTGACTGCCTGGAGGGGGC GGCCAGACGGGCTCCATCCTCTGTGGCCCACACCAGCATGAGTGACAACGGAGGCTTCAAGCGGCCCCTAGCGCCCTCAGGCCGGTCTCCAGTGGAAGGC CTGGGCAGGGCCCATCGGAGCCCTGCCTCACCAAGGGTGCCTCCGGTCCCCGACTACGTGGCACACCCCGAGCGCTGGACCAAGTACAGCCTGGAAGATG TGACCGAGGTCAGCGAGCAGAGCAATCAGGCCACCGCCCTGGCCTTCCTGGGCTCCCAGAGCCTGGCTGCCCCCACTGACTGCGTGTCCTCCTTCAACCA GGATCCCTCCAGCTGTGGGGAGGGGAGGGTCATCTTCACCAAACCAGTCCGAGGGGTCGAAGCCAGACACGAGAGGAAGAGGGTCCTGGGGAAGGTGGGA GAGCCAGGCAGGGGCGGCCTTGGGAATCCTGCCACAGACAGGGGCGAGGGCCCTGTGGAGCTGGCCCATCTGGCCGGGCCCGGGAGCCCAGAGGCTGAGG AGTGGGGCAGCCACCATGGAGGCCTGCAGGAGGTGGAGGCACTGTCAGGGTCTGTCCACAGTGGGTCTGTGCCAGGTCTCCCGCCGGTGGAAACTGTTGG CTTCCATGGCAGCAGGAAGCGGAGTCGAGACCACTTCCGGAACAAGAGCAGCAGCCCCGAGGACCCAGGTGCTGAGGTCTGAGAGGGAGATGGCCCAGCC TGACCCCACTGGCCACTGCCATCCTGCTGCCTTCCCAGTGGGGCTGGTCAGGGGGCAGCCTGGCCACTGCCTAGCTGGAATGGGAGGAAGCCTGCAGGTG GCACCGGTGGCCCTGGCTGCAGTTCTGGGCAGCATCCTCCCAAGCAGAGACCTTGCTGAAGCTCCTGGGGTGTGGGGTGTGGGCTGGAAGCACTGGCTCC CTGGTAGGGACAATAAAGGTTTTGGGTCTTTCTGAGA

TSSC4.F10 (forward primer)
TSSC4.R7 (reverse primer)
Intron retention

Frame 0

GVEE*RGERMRTGLHVPTGVGSGGPVPCGSCSS AGAEAVVLGRQSTRFPRGLSAPHGGAAPTKMETA EQP*AGRFVVREDGPRGPAEPPGGHRAQL*YLS SKAPLCSPTPVPRGRALGTHSILVRPGGLSSPT GQCRGAPRASLAPRVRLFSGGEVLLAHIVEKGRA LRSCGERDGFLQPTDMKESTLPSTRCRLSPRTP RNGLPESLSCSPIPATRRSAPLTRLLLAAAAFR CRGRECESGKQGSG*TPASSATYCVTWANYFPRP VPASFKTVISGGCCVSPFTGVASSLRRVAPQRN *GPACVRPGGGH*EPLIVPLTPSLLGAQDTGHS GRARSFGSRRGVGGWMAEDSAEGCGWAVFFCLEI QVLRHPVTPALNGCRRQHLSAQLERQGMC*VLQ VEGHWRSLQEPRGFTSA*QGCSR*WSTPHFPES LTLRCQGLGCRFLGSSRGSSSK*EQIFSPWIRKV HALCGIRLLTPHSKQLISGPPA*FQPWVQALGG CLLPKSLSSSKPACRGLAPLNEVTSQKSLPWVL ACQGLRKPRLRSVLPGTCFTHVHSGLRLVLSPTP PTCS*AAEQRPCRGTPRPETTTPVPLRTFIRAP STWPAWLSNHTPVSTTGTQQPREVWRGPGDAWL RQEQVSRPPAWRANTGRSMTRCLPTQSPSVTRTL TSACPVVLKWKHCPRWGCLGRRIQVLMSRPHPR QASSQPRCSHSI*EA*APPSPSAAVTSLTAWRG RPDGLHPLWPTPA*VTTEASSGP*RPQAGLQWKA WAGPIGALPHQGCLRSPTTWHTPSAGPSTAWKM *PRSASRAIRPPPWPSWAPRAWLPPLTACPPST RIPPAVGRGGSSSPNQSEGSKPDTRGRGSWGRWE SQAGAALGILPQTGARALWSWPIWPGPGAQRLR SGAATMEACRRWRHCQGLSTVGLCQVSRRWKLL ASMAAGSGVETTSGTRAAAPRTQVLRSEREMAQP DPTGHCHPAAFPVGLVRGQPGHCLAGMGGSLQV APVALAAVLGSILPSRDLAEAPGVWGVGWKHWL PGRDNKGFGSF*

Frame 1

AWRSRGASACAQDYTSRQASGAAAQFLVGAVVL QARKPWCSAGRALGFPEG*ARRTEVRRRPRWRL PSSLEPVGLW*GRTGRAGRPSLREVTERSFNT*A RRPRCARRPPYLAAGPLGPTASL*GPEACPARL DSAEGHREPAWHREFVCSLAGRSCWHI*WRKAG LCVHVEKETASFSLRT*RSQLYLPLVAGFRREPR ETDYRSPYLAARSPLPVGVPR*PGCFWPRRRSA AEDGSANLGSRVLVELQLRLQHTV*LGQIISPA PFLPALKRSSVGGAAYPLSLGWLLH*GESRLRGT EVLPVFDLVGGTKSP**YP*PHPYWVHKTQVTL GGQGVLVAGEESVDGWLRTVQKGVAGPSFFAWK FKF*GTQSLQH*MGAGGSTCLPSWKGRVCAECYR WKATGGRSRSRGDLPLPNRAAQGDGRHPTFLRA *PSDARALAADSLGAPGDLPANRSKSFPRGSGR CTLFVEYDCSPRTASSL*VALLPDFSPGFKPWVA AYYQNRSVAPSLPAEGWHH*MR*RVKSPYPGS* PVRGSENPGSGRSCPAPVSHMYTPV*GWSSPPP HPPAVEQLNRGHAGALRGLRRPRLCR*GPSSGLR PLGPLGCPITLQCQPLAPSSQERCGVALGTHG* GRNR*AVPQRGGRTRDGV*HAAFRHSLPQ*LGL *PQLARWC*SGSTVPDGAAWGGGFRS**AALTPV RPPPSHGAAIPSERHELHLLPAQP*HL*LPGGG GQTGSILCGPHQHE*QRRLQAAPSALRPVSSGR PGQGPSEPCLTKGASGPRLRGTPRALDQVQPGRC DRGQRAEQSGHRPGLPGLPEPGCPH*LRVLLQP GSLQLWGGEGHLHQTSPRGRSQTREEEGPGEGG RARQGRPWESCHRQGRGPCGAGPSGRAREPRG*G VGQPPWRPAGGGGTVRVCPQWVCARSPAGGNCW LPWQQEAESRPLPEQEQQPRGPRC*GLRGRWPS LTPLATAILLPSQWGWSGGSLATA*LEWEEACRW HRWPWLQFWAASSQAETLLKLLGCGVWAGSTGS LVGTIKVLGLSE

Frame 2

RGGVEGRAHAHRTTRPDRRRERRPSSLWEL*FC RRGSRGARPAEHSVSQRAERAARRCGADQDGDC RAALSR*VCGEGGRAARAGRASGRSPSAALIPE LEGPAVLADPRTSRPGPWDPQHPCEARRPVQPDW TVPRGTESQLGTESSFVLWRGGLAGTYSGERPG SAFMWRKRRLPSAYGHEGVNSTFHSLPAFAENP EKRTTGVPILQPDPRYPSECPADQAASGRGGVPL QRTGVRIWEAGFWLNSSFVCNILCDLGKLFPPP RSCQL*NGHQWGVLRIPFHWGGFFTEESRASEE LRSCLCSTWWGALRAPDSTPDPILIGCTRHRSLW AGKEFW*QERSRWMDG*GQCRRVWLGRLFLPGN SSSEAPSHSSTKWVQEAALVCPAGKAGYVLSVT GGRPLEVAPGAAGIYLCLTGLLKVMVDTPLS*EL DPQMPGPWLQIPWELPGIFQQIGANLFPVDQEG ARSLWNTTAHPAQQAAYKWPSCLISALGSSPGW LLTTKIAQ*LQACLQRVGTIK*GNESKVPTLGPS LSGAPKTQAQVGPARHLFHTCTLRSEVGPLPHP THLQLSS*TEAMPGHSEA*DDHACAAEDLHQGS VHLARLAVQSHSSVNHWHPAAKRGVAWPWGRMAE AGTGEPSPSVEGEHGTEYDTLPSDTVSLSDSDS DLSLPGGAEVEALSPMGLPGEEDSGPDEPPSPP SGLLPATVQPFHLRGMSSTFSQRSRDIFDCLEGA ARRAPSSVAHTSMSDNGGFKRPLAPSGRSPVEG LGRAHRSPASPRVPPVPDYVAHPERWTKYSLED VTEVSEQSNQATALAFLGSQSLAAPTDCVSSFNQ DPSSCGEGRVIFTKPVRGVEARHERKRVLGKVG EPGRGGLGNPATDRGEGPVELAHLAGPGSPEAE EWGSHHGGLQEVEALSGSVHSGSVPGLPPVETVG FHGSRKRSRDHFRNKSSSPEDPGAEV*EGDGPA *PHWPLPSCCLPSGAGQGAAWPLPSWNGRKPAG GTGGPGCSSGQHPPKQRPC*SSWGVGCGLEALAP W*GQ*RFWVFLR

Sequence TSSC4.bAug05

CCCTCCTCTTCCTGTTCTTGGTCAATTCCGGTCTTGTTTCCCCAACAAATGCCGTCGTTTCCGGGGCTGCTTCCGAGCCGGACCCAAGGGCCGGGGCGTG GAGGAGTAGAGGGGCGAGCGCATGCGCACAGGACTACACGTCCCGACAGGCGTCGGGAGCGGCGGCCCAGTTCCTTGTGGGAGCTGTAGTTCTGCAGGCG CGGAAGCCGTGGTGCTCGGCCGGCAGAGCACTCGGTTTCCCAGAGGGCTGAGCGCGCCGCACGGAGGTGCGGCGCCGACCAAGATGGAGACTGCCGAGCA GCCTTGAGCCGGGCTGCTCAAGGTGATGGTCGACACCCCACTTTCCTGAGAGCTTGACCCTCAGATGCCAGGGCCTTGGCTGCAGATTCCTTGGGAGCTC CCGGGGATCTTCCAGCAAATAGGAGCAAATCTTTTCCCCGTGGATCAGGAAGTTGAGCAGCTGAACAGAGGCCATGCCGGGGCACTCCGAGGCCTGAGAC GACCACGCCTGTGCCGCTGAGGACCTTCATCAGGGCTCCGTCCACTTGGCCCGCTTGGCTGTCCAATCACACTCCAGTGTCAACCACTGGCACCCAGCAG CCAAGAGAGGTGTGGCGTGGCCCTGGGGACGCATGGCTGAGGCAGGAACAGGTGAGCCGTCCCCCAGCGTGGAGGGCGAACACGGGACGGAGTATGACAC GCTGCCTTCCGACACAGTCTCCCTCAGTGACTCGGACTCTGACCTCAGCTTGCCCGGTGGTGCTGAAGTGGAAGCACTGTCCCCGATGGGGCTGCCTGGG GAGGAGGATTCAGGTCCTGATGAGCCGCCCTCACCCCCGTCAGGCCTCCTCCCAGCCACGGTGCAGCCATTCCATCTGAGAGGCATGAGCTCCACCTTCT CCCAGCGCAGCCGTGACATCTTTGACTGCCTGGAGGGGGCGGCCAGACGGGCTCCATCCTCTGTGGCCCACACCAGCATGAGTGACAACGGAGGCTTCAA GCGGCCCCTAGCGCCCTCAGGCCGGTCTCCAGTGGAAGGCCTGGGCAGGGCCCATCGGAGCCCTGCCTCACCAAGGGTGCCTCCGGTCCCCGACTACGTG GCACACCCCGAGCGCTGGACCAAGTACAGCCTGGAAGATGTGACCGAGGTCAGCGAGCAGAGCAATCAGGCCACCGCCCTGGCCTTCCTGGGCTCCCAGA GCCTGGCTGCCCCCACTGACTGCGTGTCCTCCTTCAACCAGGATCCCTCCAGCTGTGGGGAGGGGAGGGTCATCTTCACCAAACCAGTCCGAGGGGTCGA AGCCAGACACGAGAGGAAGAGGGTCCTGGGGAAGGTGGGAGAGCCAGGCAGGGGCGGCCTTGGGAATCCTGCCACAGACAGGGGCGAGGGCCCTGTGGAG CTGGCCCATCTGGCCGGGCCCGGGAGCCCAGAGGCTGAGGAGTGGGGCAGCCACCATGGAGGCCTGCAGGAGGTGGAGGCACTGTCAGGGTCTGTCCACA GTGGGTCTGTGCCAGGTCTCCCGCCGGTGGAAACTGTTGGCTTCCATGGCAGCAGGAAGCGGAGTCGAGACCACTTCCGGAACAAGAGCAGCAGCCCCGA GGACCCAGGTGCTGAGGTCTGAGAGGGAGATGGCCCAGCCTGACCCCACTGGCCACTGCCATCCTGCTGCCTTCCCAGTGGGGCTGGTCAGGGGGCAGCC TGGCCACTGCCTAGCTGGAATGGGAGGAAGCCTGCAGGTGGCACCGGTGGCCCTGGCTGCAGTTCTGGGCAGCATCCTCCCAAGCAGAGACCTTGCTGAA GCTCCTGGGGTGTGGGGTGTGGGCTGGAAGCACTGGCTCCCTGGTAGGGACAATAAAGGTTTTGGGTCTTTCTGAGA

TSSC4.F10 (forward primer)
TSSC4.R7 (reverse primer)
Intron retention

Frame 0

PSSSCSWSIPVLFPQQMPSFPGLLPSRTQGPGR GGVEGRAHAHRTTRPDRRRERRPSSLWEL*FCRR GSRGARPAEHSVSQRAERAARRCGADQDGDCRA ALSRAAQGDGRHPTFLRA*PSDARALAADSLGA PGDLPANRSKSFPRGSGS*AAEQRPCRGTPRPET TTPVPLRTFIRAPSTWPAWLSNHTPVSTTGTQQ PREVWRGPGDAWLRQEQVSRPPAWRANTGRSMT RCLPTQSPSVTRTLTSACPVVLKWKHCPRWGCLG RRIQVLMSRPHPRQASSQPRCSHSI*EA*APPS PSAAVTSLTAWRGRPDGLHPLWPTPA*VTTEAS SGP*RPQAGLQWKAWAGPIGALPHQGCLRSPTTW HTPSAGPSTAWKM*PRSASRAIRPPPWPSWAPR AWLPPLTACPPSTRIPPAVGRGGSSSPNQSEGS KPDTRGRGSWGRWESQAGAALGILPQTGARALWS WPIWPGPGAQRLRSGAATMEACRRWRHCQGLST VGLCQVSRRWKLLASMAAGSGVETTSGTRAAAP RTQVLRSEREMAQPDPTGHCHPAAFPVGLVRGQP GHCLAGMGGSLQVAPVALAAVLGSILPSRDLAE APGVWGVGWKHWLPGRDNKGFGSF*

Frame 1

PPLPVLGQFRSCFPNKCRRFRGCFRAGPKGRGV EE*RGERMRTGLHVPTGVGSGGPVPCGSCSSAG AEAVVLGRQSTRFPRGLSAPHGGAAPTKMETAEQ P*AGLLKVMVDTPLS*ELDPQMPGPWLQIPWEL PGIFQQIGANLFPVDQEVEQLNRGHAGALRGLR RPRLCR*GPSSGLRPLGPLGCPITLQCQPLAPSS QERCGVALGTHG*GRNR*AVPQRGGRTRDGV*H AAFRHSLPQ*LGL*PQLARWC*SGSTVPDGAAW GGGFRS**AALTPVRPPPSHGAAIPSERHELHLL PAQP*HL*LPGGGGQTGSILCGPHQHE*QRRLQ AAPSALRPVSSGRPGQGPSEPCLTKGASGPRLR GTPRALDQVQPGRCDRGQRAEQSGHRPGLPGLPE PGCPH*LRVLLQPGSLQLWGGEGHLHQTSPRGR SQTREEEGPGEGGRARQGRPWESCHRQGRGPCG AGPSGRAREPRG*GVGQPPWRPAGGGGTVRVCPQ WVCARSPAGGNCWLPWQQEAESRPLPEQEQQPR GPRC*GLRGRWPSLTPLATAILLPSQWGWSGGS LATA*LEWEEACRWHRWPWLQFWAASSQAETLLK LLGCGVWAGSTGSLVGTIKVLGLSE

Frame 2

LLFLFLVNSGLVSPTNAVVSGAASEPDPRAGAW RSRGASACAQDYTSRQASGAAAQFLVGAVVLQA RKPWCSAGRALGFPEG*ARRTEVRRRPRWRLPS SLEPGCSR*WSTPHFPESLTLRCQGLGCRFLGSS RGSSSK*EQIFSPWIRKLSS*TEAMPGHSEA*D DHACAAEDLHQGSVHLARLAVQSHSSVNHWHPA AKRGVAWPWGRMAEAGTGEPSPSVEGEHGTEYDT LPSDTVSLSDSDSDLSLPGGAEVEALSPMGLPG EEDSGPDEPPSPPSGLLPATVQPFHLRGMSSTF SQRSRDIFDCLEGAARRAPSSVAHTSMSDNGGFK RPLAPSGRSPVEGLGRAHRSPASPRVPPVPDYV AHPERWTKYSLEDVTEVSEQSNQATALAFLGSQ SLAAPTDCVSSFNQDPSSCGEGRVIFTKPVRGVE ARHERKRVLGKVGEPGRGGLGNPATDRGEGPVE LAHLAGPGSPEAEEWGSHHGGLQEVEALSGSVH SGSVPGLPPVETVGFHGSRKRSRDHFRNKSSSPE DPGAEV*EGDGPA*PHWPLPSCCLPSGAGQGAA WPLPSWNGRKPAGGTGGPGCSSGQHPPKQRPC* SSWGVGCGLEALAPW*GQ*RFWVFLR

Sequence TSSC4.aAug05

CCCTCCTCTTCCTGTTCTTGGTCAATTCCGGTCTTGTTTCCCCAACAAATGCCGTCGTTTCCGGGGCTGCTTCCGAGCCGGACCCAAGGGCCGGGGCGTG GAGGAGTAGAGGGGCGAGCGCATGCGCACAGGACTACACGTCCCGACAGGCGTCGGGAGCGGCGGCCCAGTTCCTTGTGGGAGCTGTAGTTCTGCAGGCG CGGAAGCCGTGGTGCTCGGCCGGCAGAGCACTCGGTTTCCCAGAGGGCTGAGCGCGCCGCACGGAGGTGCGGCGCCGACCAAGATGGAGACTGCCGAGCA GCCTTGAGCCGTTGAGCAGCTGAACAGAGGCCATGCCGGGGCACTCCGAGGCCTGAGACGACCACGCCTGTGCCGCTGAGGACCTTCATCAGGGCTCCGT CCACTTGGCCCGCTTGGCTGTCCAATCACACTCCAGTGTCAACCACTGGCACCCAGCAGCCAAGAGAGGTGTGGCGTGGCCCTGGGGACGCATGGCTGAG GCAGGAACAGGTGAGCCGTCCCCCAGCGTGGAGGGCGAACACGGGACGGAGTATGACACGCTGCCTTCCGACACAGTCTCCCTCAGTGACTCGGACTCTG ACCTCAGCTTGCCCGGTGGTGCTGAAGTGGAAGCACTGTCCCCGATGGGGCTGCCTGGGGAGGAGGATTCAGGTCCTGATGAGCCGCCCTCACCCCCGTC AGGCCTCCTCCCAGCCACGGTGCAGCCATTCCATCTGAGAGGCATGAGCTCCACCTTCTCCCAGCGCAGCCGTGACATCTTTGACTGCCTGGAGGGGGCG GCCAGACGGGCTCCATCCTCTGTGGCCCACACCAGCATGAGTGACAACGGAGGCTTCAAGCGGCCCCTAGCGCCCTCAGGCCGGTCTCCAGTGGAAGGCC TGGGCAGGGCCCATCGGAGCCCTGCCTCACCAAGGGTGCCTCCGGTCCCCGACTACGTGGCACACCCCGAGCGCTGGACCAAGTACAGCCTGGAAGATGT GACCGAGGTCAGCGAGCAGAGCAATCAGGCCACCGCCCTGGCCTTCCTGGGCTCCCAGAGCCTGGCTGCCCCCACTGACTGCGTGTCCTCCTTCAACCAG GATCCCTCCAGCTGTGGGGAGGGGAGGGTCATCTTCACCAAACCAGTCCGAGGGGTCGAAGCCAGACACGAGAGGAAGAGGGTCCTGGGGAAGGTGGGAG AGCCAGGCAGGGGCGGCCTTGGGAATCCTGCCACAGACAGGGGCGAGGGCCCTGTGGAGCTGGCCCATCTGGCCGGGCCCGGGAGCCCAGAGGCTGAGGA GTGGGGCAGCCACCATGGAGGCCTGCAGGAGGTGGAGGCACTGTCAGGGTCTGTCCACAGTGGGTCTGTGCCAGGTCTCCCGCCGGTGGAAACTGTTGGC TTCCATGGCAGCAGGAAGCGGAGTCGAGACCACTTCCGGAACAAGAGCAGCAGCCCCGAGGACCCAGGTGCTGAGGTCTGAGAGGGAGATGGCCCAGCCT GACCCCACTGGCCACTGCCATCCTGCTGCCTTCCCAGTGGGGCTGGTCAGGGGGCAGCCTGGCCACTGCCTAGCTGGAATGGGAGGAAGCCTGCAGGTGG CACCGGTGGCCCTGGCTGCAGTTCTGGGCAGCATCCTCCCAAGCAGAGACCTTGCTGAAGCTCCTGGGGTGTGGGGTGTGGGCTGGAAGCACTGGCTCCC TGGTAGGGACAATAAAGGTTTTGGGTCTTTCTGAGA

TSSC4.F10 (forward primer)
TSSC4.R7 (reverse primer)
Intron retention

Frame 0

PSSSCSWSIPVLFPQQMPSFPGLLPSRTQGPGR GGVEGRAHAHRTTRPDRRRERRPSSLWEL*FCRR GSRGARPAEHSVSQRAERAARRCGADQDGDCRA ALSR*AAEQRPCRGTPRPETTTPVPLRTFIRAP STWPAWLSNHTPVSTTGTQQPREVWRGPGDAWLR QEQVSRPPAWRANTGRSMTRCLPTQSPSVTRTL TSACPVVLKWKHCPRWGCLGRRIQVLMSRPHPR QASSQPRCSHSI*EA*APPSPSAAVTSLTAWRGR PDGLHPLWPTPA*VTTEASSGP*RPQAGLQWKA WAGPIGALPHQGCLRSPTTWHTPSAGPSTAWKM *PRSASRAIRPPPWPSWAPRAWLPPLTACPPSTR IPPAVGRGGSSSPNQSEGSKPDTRGRGSWGRWE SQAGAALGILPQTGARALWSWPIWPGPGAQRLR SGAATMEACRRWRHCQGLSTVGLCQVSRRWKLLA SMAAGSGVETTSGTRAAAPRTQVLRSEREMAQP DPTGHCHPAAFPVGLVRGQPGHCLAGMGGSLQV APVALAAVLGSILPSRDLAEAPGVWGVGWKHWLP GRDNKGFGSF*

Frame 1

PPLPVLGQFRSCFPNKCRRFRGCFRAGPKGRGV EE*RGERMRTGLHVPTGVGSGGPVPCGSCSSAG AEAVVLGRQSTRFPRGLSAPHGGAAPTKMETAEQ P*AVEQLNRGHAGALRGLRRPRLCR*GPSSGLR PLGPLGCPITLQCQPLAPSSQERCGVALGTHG* GRNR*AVPQRGGRTRDGV*HAAFRHSLPQ*LGL* PQLARWC*SGSTVPDGAAWGGGFRS**AALTPV RPPPSHGAAIPSERHELHLLPAQP*HL*LPGGG GQTGSILCGPHQHE*QRRLQAAPSALRPVSSGRP GQGPSEPCLTKGASGPRLRGTPRALDQVQPGRC DRGQRAEQSGHRPGLPGLPEPGCPH*LRVLLQP GSLQLWGGEGHLHQTSPRGRSQTREEEGPGEGGR ARQGRPWESCHRQGRGPCGAGPSGRAREPRG*G VGQPPWRPAGGGGTVRVCPQWVCARSPAGGNCW LPWQQEAESRPLPEQEQQPRGPRC*GLRGRWPSL TPLATAILLPSQWGWSGGSLATA*LEWEEACRW HRWPWLQFWAASSQAETLLKLLGCGVWAGSTGS LVGTIKVLGLSE

Frame 2

LLFLFLVNSGLVSPTNAVVSGAASEPDPRAGAW RSRGASACAQDYTSRQASGAAAQFLVGAVVLQA RKPWCSAGRALGFPEG*ARRTEVRRRPRWRLPS SLEPLSS*TEAMPGHSEA*DDHACAAEDLHQGSV HLARLAVQSHSSVNHWHPAAKRGVAWPWGRMAE AGTGEPSPSVEGEHGTEYDTLPSDTVSLSDSDS DLSLPGGAEVEALSPMGLPGEEDSGPDEPPSPPS GLLPATVQPFHLRGMSSTFSQRSRDIFDCLEGA ARRAPSSVAHTSMSDNGGFKRPLAPSGRSPVEG LGRAHRSPASPRVPPVPDYVAHPERWTKYSLEDV TEVSEQSNQATALAFLGSQSLAAPTDCVSSFNQ DPSSCGEGRVIFTKPVRGVEARHERKRVLGKVG EPGRGGLGNPATDRGEGPVELAHLAGPGSPEAEE WGSHHGGLQEVEALSGSVHSGSVPGLPPVETVG FHGSRKRSRDHFRNKSSSPEDPGAEV*EGDGPA *PHWPLPSCCLPSGAGQGAAWPLPSWNGRKPAGG TGGPGCSSGQHPPKQRPC*SSWGVGCGLEALAP W*GQ*RFWVFLR