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Sequences

Sequence SYK.dAug05

CTGAGGCCACCCCGGCGGCGGCTGGAGAGCGAGGAGGAGCGGGTGGCCCCGCGCTGCGCCCGCCCTCGCCTCACCTGGCGCAGGTAGGTGTGGCCGCGTC CCCTACCCGGCCGGGACTTTCTGGTAAGGAGAGGAGGTTACGGGGAACGACGCGCTGCTTTCATGCCCTTTCTTGTTCTACCTTCATCGGCCGAGGTGGA CACCTGCGCAGGTGTGTGCCCTCCGGCCCCTGAAGCATGGCCAGCAGCGGCATGGCTGACAGCGCCAACCACCTGCCCTTCTTTTTCGGCAACATCACCC GGGAGGAGGCAGAAGATTACCTGGTCCAGGGGGGCATGAGTGATGGGCTTTATTTGCTGCGCCAGAGCCGCAACTACCTGGGTGGCTTCGCCCTGTCCGT GGCCCACGGGAGGAAGGCACACCACTACACCATCGAGCGGGAGCTGAATGGCACCTACGCCATCGCCGGTGGCAGGACCCATGCCAGCCCCGCCGACCTC TGCCACTACCACTCCCAGGAGTCTGATGGCCTGGTCTGCCTCCTCAAGAAGCCCTTCAACCGGCCCCAAGGGGTGCAGCCCAAGACTGGGCCCTTTGAGG ATTTGAAGGAAAACCTCATCAGGGAATATGTGAAGCAGACATGGAACCTGCAGGGTCAGGCTCTGGAGCAGGCCATCATCAGTCAGAAGCCTCAGCTGGA GAAGCTGATCGCTACCACAGCCCATGAAAAAATGCCTTGGTTCCATGGAAAAATCTCTCGGGAAGAATCTGAGCAAATTGTCCTGATAGGATCAAAGACA AATGGAAAGTTCCTGATCCGAGCCAGAGACAACAACGGCTCCTACGCCCTGTGCCTGCTGCACGAAGGGAAGGTGCTGCACTATCGCATCGACAAAGACA AGACAGGGAAGCTCTCCATCCCCGAGGGAAAGAAGTTCGACACGCTCTGGCAGCTAGTCGAGCATTATTCTTATAAAGCAGATGGTTTGTTAAGAGTTCT TACTGTCCCATGTCAAAAAATCGGCACACAGGGAAATGTTAATTTTGGAGGCCGTCCACAACTTCCAGGTTCCCATCCTGCGTCCTCCCCTGCCCAAGGG AACCGGCAAGAGAGTACTGTGTCATTCAATCCGTATGAGCCAGAACTTGCACCCTGGGCTGCAGACAAAGGCCCCCAGAGAGAAGCCCTACCCATGGACA CAGAGGTGTACGAGAGCCCCTACGCGGACCCCGAGGAGATCAGGCCCAAGGAGGTTTACCTGGACCGAAAGCTGCTGACGCTGGAAGACAAAGAACTGGG CTCTGGTAATTTTGGAACTGTGAAAAAGGGCTACTACCAAATGAAAAAAGTTGTGAAAACCGTGGCTGTGAAAATACTGAAAAACGAGGCCAATGACCCC GCTCTTAAAGATGAGTTATTAGCAGAAGCAAATGTCATGCAGCAGCTGGACAACCCGTACATCGTGCGGATGATCGGGATATGCGAGGCCGAGTCCTGGA TGCTGGTTATGGAGATGGCAGAACTTGGTCCCCTCAATAAGTATTTGCAGCAGAACAGACATGTCAAGGATAAGAACATCATAGAACTGGTTCATCAGGT TTCCATGGGCATGAAGTACTTGGAGGAGAGCAATTTTGTGCACAGAGATCTGGCTGCAAGAAATGTGTTGCTAGTTACCCAACATTACGCCAAGATCAGT GATTTCGGACTTTCCAAAGCACTGCGTGCTGATGAAAACTACTACAAGGCCCAGACCCATGGAAAGTGGCCTGTCAAGTGGTACGCTCCGGAATGCATCA ACTACTACAAGTTCTCCAGCAAAAGCGATGTCTGGAGCTTTGGAGTGTTGATGTGGGAAGCATTCTCCTATGGGCAGAAGCCATATCGAGGGATGAAAGG AAGTGAAGTCACCGCTATGTTAGAGAAAGGAGAGCGGATGGGGTGCCCTGCAGGGTGTCCAAGAGAGATGTACGATCTCATGAATCTGTGCTGGACATAC GATGTGGAAAACAGGCCCGGATTCGCAGCAGTGGAACTGCGGCTGCGCAATTACTACTATGACGTGGTGAACTAACCGCTCCCGCACCTGTCGGTGGCTG CCTTTGATCACAGGAGCAATCACAGGAAAATGTATCCAGAGGAATTGATTGTCAGCCACCTCCCTCTGCCAGTCGGGAGAGCCAGGCTTGGATGGAACAT GCCCACAACTTGTCACCCAAAGCCTGTCCCAGGACTCACCCTCCACAAAGCAAAGGCAGTCCCGGGAGAAAAGACGGATGGCAGGATCCAAGGGGCTAGC TGGATTTGTTTGTTTTCTTGTCTGTGTGATTTTCATACAGGTTATTTTTACGATCTGTTTCCAAATCCCTTTCATGTCTTTCCACTTCTCTGGGTCCCGG GGTGCATTTGTTACTCATCGGGCCCAGGGACATTGCAGAGTGGCCTAGAGCACTCTCACCCCAAGCGGCCTTTTCCAAATGCCCAAGGATGCCTTAGCAT GTGACTCCTGAAGGGAAGGCAAAGGCAGAGGAATTTGGCTGCTTCTACGGCCATGAGACTGATCCCTGGCCACTGAAAAGCTTTCCTGACAATAAAAATG TTTTGAGGCTTTAAAAAGAAAATCAAGTTTGACCAGTGCAGTTTCTAAGCATGTAGCCAGTTAAGGAAAGAAAGAAAGAAAAAAAAAAAAGGCCTGGATA CTGCTTTTGCTGTCTCTGTTATGAGATGGAAGACTTACATGTTTGTGATAAAAGGGGACCATGAGAATGAATTGGCTTGGCTTACTTTCCCCCTGAAATC CTCTCTCCTGCAGACTGTCTTGAAGACCTGGTGACTGGTAAATAAAGCCCTGCATGGAGGCTGCACAGCAGGGGCAAGAGGCCCATCCCCCAGCATCTCA CTGAGGACAGCTTCAGGCTGCCTTCCTCTGAACGTGGTCCACACCTTCCTCTCCTCCACAGAGAGGGTGCCGCCAGAATCCCCTGTCGCTTTCTGTGTCT GCAATGGGGGGCAGCACAGGGATCAAAGCCATCTAAAGAGTTTCCAAAGAAAGTATTAATTCAGAACAAGCCAAAGACCCTGAGCCTCACCACAAACAGG CCTTTTGGAGTGTGAATTTGAGTTGAAGATACAAGATCGGAGAATGATTTTCTGGTCTTAACTAATCCTCATCTTCATGTTTGATCTTTAAGAAGTCATC ACCCATTGATTTCAGTTTTGCTGTACCTCTTGAAAGTTAAAGAGACATCTCAGCACTTTAGGAGGCCGAGGCGGGTGGATCACTTGAGGTAAGGAGTTTG AGACTAGCCTGGCCAATATGGTAAAACCCCATCTCTACTAAAAATACAAAAATTAGCCGGGCATGGTGGCATGTGTCTGTAGTCCCAGCTACTCGGGAGG CTGAGGCAGGAGAATCGCTTGAACCCAGGAAACGGAGGTCGCAGTGAGCCAAGATCATGCCACTGCACTCCAGCTTGGGCATCACAGCGAGACTCTGTCA AAACAAACAAACAAAAAAACAACTTAAAGAGGTAATTTAGCCATCATTCTTATGCCAGCAGATATAAATAAACTTGGACCCATCTGGTCTTCAGCTAAAC CTGAGACATTTTAAAGTGCATGGACAGCCATGGACAGCAGGCCCTCCTCTAACAGGGGATGCAAGGCATGGAGAAAGACAATCAGTACCCAAGCTCAGCC ACAGAAGACAGGAGTCACTCATATAACTTGTGTTTAGAAGTTTTTGGTAGCCACGCACACTTTCTGAAATCACACTATCTGGTGGTTTAATCATATTTTT AAAGACAGAATCCCTGAGTGCTGAGCAGATTCTCAAAACACATTTAGAATCCCTGAAATTAGAAAGATCAATGACAAAATATCTGTCAGCCAGGCCACAA ACAGGTGTAAAATTATGAAAGGAGTGGTTGGATGTGCCAAGTTTGGTAAAGTGGTGACTGCATCTGAGAAAGAGGCTGTGAGGCTGAACTCTTGGTGGCT TCCTTCTGTAACTTCCAGAGGGAGTCTTCAACACAGGCCCCGTGCTCGTAGGAATACGGTAGCACCTATGTAGGAAGTGCGTGGAGTTTTCTGTCTTCTT TCTGTGTGATTTTTGGCCTTTTTATCAGCACTTCTCCCCTCCCAGGAGCCTGGGGATGCCAAACATCCAGAATGTGATGGGACAAGATGGGGGCAGGGGC CTCACCTCCCTGCAGAGGTCCGGCCAGGTCTCCTTGTCCCTGGACAATCTCCTGAGCCTCTCTGCTTGGTGGAGCAGGCACCTGTGTGCAGAATTCCCAC TGTGGCCAGCACGAGGAAGTCTTTTCTAGTGAAAATGTGTCTTGTGGTCAGGAATAATTATCCTTTCCCCTGTAGCCACCAAGGAGGGCAAATAGAGAAA GGTAACCTAATTGAAGGATTGGTCATGTGAAAAGGGCTACATTTGGGAAGCTGGGAAAGGCCTCCAGGCTTCTAGAGCAGCTAGCTTGGGCTGGATTCTC ATACCCAGGCTGCCCCTTGGATTGTTCTACCCAAGCTTTTCCCTGGGGTCTGGGCTCACTCCATAAGGTAAGGTGCCTTTTACCTTATGGTCCTTCTTTA GCAGGTAACAAAGGAGCATCAGGGGCAGGCTGCCCTGGTGGCATCACACTGGCTAGTGAGGCCGTGAATATCTTGTCCCCCAGCAGGGCCGACAGTTTCT ATCACAGAAAACAGTGTGTTCAGTGGTGAAAATCGTTGCATGCATGTTTTCATCTGAGCGTGTCCTTCTCCCATACTCCCTATCAGCCAGCCCTGCCTGT AGCTGCTGTATGGTGATTGCACTTGGACATCAGTCCAATGACTGCAAGTCGGCCTGGATTTTCACTTGCAGAGGCTACAGCTGCATTGTCAGGTCTCCCA GCCCTGCAGAGAGCTCCCTCCACTGGTTAGCAGTGTGTTGTGTTTTCCATTCATTTCAGAAGAGCTACATTGTGTCACTGGACATTTTTAAAAACTGTGA TTTTTAATAAAAATTTAAAATTTGCTTTGTGATGA

SYK.F1 (forward primer)
SYK.R16 (reverse primer)
Single exon skipping

Frame 0

LRPPRRRLESEEERVAPRCARPRLTWRR*VWPR PLPGRDFLVRRGGYGERRAAFMPFLVLPSSAEVD TCAGVCPPAPEAWPAAAWLTAPTTCPSFSATSP GRRQKITWSRGA*VMGFICCARAATTWVASPCP WPTGGRHTTTPSSGS*MAPTPSPVAGPMPAPPTS ATTTPRSLMAWSASSRSPSTGPKGCSPRLGPLR I*RKTSSGNM*SRHGTCRVRLWSRPSSVRSLSW RS*SLPQPMKKCLGSMEKSLGKNLSKLS**DQRQ MESS*SEPETTTAPTPCACCTKGRCCTIASTKT RQGSSPSPRERSSTRSGS*SSIILIKQMVC*EF LLSHVKKSAHREMLILEAVHNFQVPILRPPLPKG TGKRVLCHSIRMSQNLHPGLQTKAPREKPYPWT QRCTRAPTRTPRRSGPRRFTWTESC*RWKTKNW ALVILEL*KRATTK*KKL*KPWL*KY*KTRPMTP LLKMSY*QKQMSCSSWTTRTSCG*SGYARPSPG CWLWRWQNLVPSISICSRTDMSRIRTS*NWFIR FPWA*STWRRAILCTEIWLQEMCC*LPNITPRSV ISDFPKHCVLMKTTTRPRPMESGLSSGTLRNAS TTTSSPAKAMSGALEC*CGKHSPMGRSHIEG*K EVKSPLC*RKESGWGALQGVQERCTIS*ICAGHT MWKTGPDSQQWNCGCAITTMTW*TNRSRTCRWL PLITGAITGKCIQRN*LSATSLCQSGEPGLDGT CPQLVTQSLSQDSPSTKQRQSREKRRMAGSKGLA GFVCFLVCVIFIQVIFTICFQIPFMSFHFSGSR GAFVTHRAQGHCRVA*STLTPSGLFQMPKDALA CDS*REGKGRGIWLLLRP*D*SLATEKLS*Q*KC FEALKRKSSLTSAVSKHVAS*GKKERKKKKAWI LLLLSLL*DGRLTCL**KGTMRMNWLGLLSP*N PLSCRLS*RPGDW*IKPCMEAAQQGQEAHPPASH *GQLQAAFL*TWSTPSSPPQRGCRQNPLSLSVS AMGGSTGIKAI*RVSKESINSEQAKDPEPHHKQ AFWSVNLS*RYKIGE*FSGLN*SSSSCLIFKKSS PIDFSFAVPLES*RDISAL*EAEAGGSLEVRSL RLAWPIW*NPISTKNTKISRAWWHVSVVPATRE AEAGESLEPRKRRSQ*AKIMPLHSSLGITARLCQ NKQTKKQLKEVI*PSFLCQQI*INLDPSGLQLN LRHFKVHGQPWTAGPPLTGDARHGERQSVPKLS HRRQESLI*LVFRSFW*PRTLSEITLSGGLIIFL KTESLSAEQILKTHLESLKLERSMTKYLSARPQ TGVKL*KEWLDVPSLVKW*LHLRKRL*G*TLGG FLL*LPEGVFNTGPVLVGIR*HLCRKCVEFSVFF LCDFWPFYQHFSPPRSLGMPNIQNVMGQDGGRG LTSLQRSGQVSLSLDNLLSLSAWWSRHLCAEFP LWPARGSLF**KCVLWSGIIILSPVATKEGK*RK VT*LKDWSCEKGYIWEAGKGLQASRAASLGWIL IPRLPLGLFYPSFSLGSGLTP*GKVPFTLWSFF SR*QRSIRGRLPWWHHTG**GREYLVPQQGRQFL SQKTVCSVVKIVACMFSSERVLLPYSLSASPAC SCCMVIALGHQSNDCKSAWIFTCRGYSCIVRSP SPAESSLHWLAVCCVFHSFQKSYIVSLDIFKNCD F**KFKICFVM

Frame 1

*GHPGGGWRARRSGWPRAAPALASPGAGRCGRV PYPAGTFW*GEEVTGNDALLSCPFLFYLHRPRW TPAQVCALRPLKHGQQRHG*QRQPPALLFRQHHP GGGRRLPGPGGHE*WALFAAPEPQLPGWLRPVR GPREEGTPLHHRAGAEWHLRHRRWQDPCQPRRP LPLPLPGV*WPGLPPQEALQPAPRGAAQDWAL*G FEGKPHQGICEADMEPAGSGSGAGHHQSEASAG EADRYHSP*KNALVPWKNLSGRI*ANCPDRIKD KWKVPDPSQRQQRLLRPVPAARREGAALSHRQRQ DREALHPRGKEVRHALAASRALFL*SRWFVKSS YCPMSKNRHTGKC*FWRPSTTSRFPSCVLPCPR EPAREYCVIQSV*ARTCTLGCRQRPPERSPTHGH RGVREPLRGPRGDQAQGGLPGPKAADAGRQRTG LW*FWNCEKGLLPNEKSCENRGCENTEKRGQ*P RS*R*VISRSKCHAAAGQPVHRADDRDMRGRVLD AGYGDGRTWSPQ*VFAAEQTCQG*EHHRTGSSG FHGHEVLGGEQFCAQRSGCKKCVASYPTLRQDQ *FRTFQSTAC**KLLQGPDPWKVACQVVRSGMHQ LLQVLQQKRCLELWSVDVGSILLWAEAISRDER K*SHRYVRERRADGVPCRVSKRDVRSHESVLDI RCGKQARIRSSGTAAAQLLL*RGELTAPAPVGGC L*SQEQSQENVSRGIDCQPPPSASRESQAWMEH AHNLSPKACPRTHPPQSKGSPGRKDGWQDPRG* LDLFVFLSV*FSYRLFLRSVSKSLSCLSTSLGPG VHLLLIGPRDIAEWPRALSPQAAFSKCPRMP*H VTPEGKAKAEEFGCFYGHETDPWPLKSFPDNKN VLRL*KENQV*PVQFLSM*PVKERKKEKKKRPGY CFCCLCYEMEDLHVCDKRGP*E*IGLAYFPPEI LSPADCLEDLVTGK*SPAWRLHSRGKRPIPQHL TEDSFRLPSSERGPHLPLLHREGAARIPCRFLCL QWGAAQGSKPSKEFPKKVLIQNKPKTLSLTTNR PFGV*I*VEDTRSENDFLVLTNPHLHV*SLRSH HPLISVLLYLLKVKETSQHFRRPRRVDHLR*GV* D*PGQYGKTPSLLKIQKLAGHGGMCL*SQLLGR LRQENRLNPGNGGRSEPRSCHCTPAWASQRDSV KTNKQKNNLKR*FSHHSYASRYK*TWTHLVFS*T *DILKCMDSHGQQALL*QGMQGMEKDNQYPSSA TEDRSHSYNLCLEVFGSHAHFLKSHYLVV*SYF *RQNP*VLSRFSKHI*NP*N*KDQ*QNICQPGHK QV*NYERSGWMCQVW*SGDCI*ERGCEAELLVA SFCNFQRESSTQAPCS*EYGSTYVGSAWSFLSS FCVIFGLFISTSPLPGAWGCQTSRM*WDKMGAGA SPPCRGPARSPCPWTIS*ASLLGGAGTCVQNSH CGQHEEVFSSENVSCGQE*LSFPL*PPRRANRE R*PN*RIGHVKRATFGKLGKASRLLEQLAWAGFS YPGCPLDCSTQAFPWGLGSLHKVRCLLPYGPSL AGNKGASGAGCPGGITLASEAVNILSPSRADSF YHRKQCVQW*KSLHACFHLSVSFSHTPYQPALPV AAVW*LHLDISPMTASRPGFSLAEATAALSGLP ALQRAPSTG*QCVVFSIHFRRATLCHWTFLKTV IFNKNLKFAL**

Frame 2

EATPAAAGERGGAGGPALRPPSPHLAQVGVAAS PTRPGLSGKERRLRGTTRCFHALSCSTFIGRGG HLRRCVPSGP*SMASSGMADSANHLPFFFGNIT REEAEDYLVQGGMSDGLYLLRQSRNYLGGFALSV AHGRKAHHYTIERELNGTYAIAGGRTHASPADL CHYHSQESDGLVCLLKKPFNRPQGVQPKTGPFE DLKENLIREYVKQTWNLQGQALEQAIISQKPQLE KLIATTAHEKMPWFHGKISREESEQIVLIGSKT NGKFLIRARDNNGSYALCLLHEGKVLHYRIDKD KTGKLSIPEGKKFDTLWQLVEHYSYKADGLLRVL TVPCQKIGTQGNVNFGGRPQLPGSHPASSPAQG NRQESTVSFNPYEPELAPWAADKGPQREALPMD TEVYESPYADPEEIRPKEVYLDRKLLTLEDKELG SGNFGTVKKGYYQMKKVVKTVAVKILKNEANDP ALKDELLAEANVMQQLDNPYIVRMIGICEAESW MLVMEMAELGPLNKYLQQNRHVKDKNIIELVHQV SMGMKYLEESNFVHRDLAARNVLLVTQHYAKIS DFGLSKALRADENYYKAQTHGKWPVKWYAPECI NYYKFSSKSDVWSFGVLMWEAFSYGQKPYRGMKG SEVTAMLEKGERMGCPAGCPREMYDLMNLCWTY DVENRPGFAAVELRLRNYYYDVVN*PLPHLSVA AFDHRSNHRKMYPEELIVSHLPLPVGRARLGWNM PTTCHPKPVPGLTLHKAKAVPGEKTDGRIQGAS WICLFSCLCDFHTGYFYDLFPNPFHVFPLLWVP GCICYSSGPGTLQSGLEHSHPKRPFPNAQGCLSM *LLKGRQRQRNLAASTAMRLIPGH*KAFLTIKM F*GFKKKIKFDQCSF*ACSQLRKERKKKKKGLD TAFAVSVMRWKTYMFVIKGDHENELAWLTFPLKS SLLQTVLKTW*LVNKALHGGCTAGARGPSPSIS LRTASGCLPLNVVHTFLSSTERVPPESPVAFCV CNGGQHRDQSHLKSFQRKY*FRTSQRP*ASPQTG LLECEFELKIQDRRMIFWS*LILIFMFDL*EVI TH*FQFCCTS*KLKRHLSTLGGRGGWIT*GKEF ETSLANMVKPHLY*KYKN*PGMVACVCSPSYSGG *GRRIA*TQETEVAVSQDHATALQLGHHSETLS KQTNKKTT*RGNLAIILMPADINKLGPIWSSAK PETF*SAWTAMDSRPSSNRGCKAWRKTISTQAQP QKTGVTHITCV*KFLVATHTF*NHTIWWFNHIF KDRIPEC*ADSQNTFRIPEIRKINDKISVSQAT NRCKIMKGVVGCAKFGKVVTASEKEAVRLNSWWL PSVTSRGSLQHRPRARRNTVAPM*EVRGVFCLL SV*FLAFLSALLPSQEPGDAKHPECDGTRWGQG PHLPAEVRPGLLVPGQSPEPLCLVEQAPVCRIPT VASTRKSFLVKMCLVVRNNYPFPCSHQGGQIEK GNLIEGLVM*KGLHLGSWERPPGF*SS*LGLDS HTQAAPWIVLPKLFPGVWAHSIR*GAFYLMVLL* QVTKEHQGQAALVASHWLVRP*ISCPPAGPTVS ITENSVFSGENRCMHVFI*ACPSPILPISQPCL *LLYGDCTWTSVQ*LQVGLDFHLQRLQLHCQVSQ PCRELPPLVSSVLCFPFISEELHCVTGHF*KL* FLIKI*NLLCD

Sequence SYK.cAug05

TTGCCCAAAATGAGGAAGAGCCGCGGGCCCGGCGGCTGAGGCCACCCCGGCGGCGGCTGGAGAGCGAGGAGGAGCGGGTGGCCCCGCGCTGCGCCCGCCC TCGCCTCACCTGGCGCAGGTGGACACCTGCGCAGGTGTGTGCCCTCCGGCCCCTGAAGCATGGCCAGCAGCGGCATGGCTGACAGCGCCAACCACCTGCC CTTCTTTTTCGGCAACATCACCCGGGAGGAGGCAGAAGATTACCTGGTCCAGGGGGGCATGAGTGATGGGCTTTATTTGCTGCGCCAGAGCCGCAACTAC CTGGGTGGCTTCGCCCTGTCCGTGGCCCACGGGAGGAAGGCACACCACTACACCATCGAGCGGGAGCTGAATGGCACCTACGCCATCGCCGGTGGCAGGA CCCATGCCAGCCCCGCCGACCTCTGCCACTACCACTCCCAGGAGTCTGATGGCCTGGTCTGCCTCCTCAAGAAGCCCTTCAACCGGCCCCAAGGGGTGCA GCCCAAGACTGGGCCCTTTGAGGATTTGAAGGAAAACCTCATCAGGGAATATGTGAAGCAGACATGGAACCTGCAGGGTCAGGCTCTGGAGCAGGCCATC ATCAGTCAGAAGCCTCAGCTGGAGAAGCTGATCGCTACCACAGCCCATGAAAAAATGCCTTGGTTCCATGGAAAAATCTCTCGGGAAGAATCTGAGCAAA TTGTCCTGATAGGATCAAAGACAAATGGAAAGTTCCTGATCCGAGCCAGAGACAACAACGGCTCCTACGCCCTGTGCCTGCTGCACGAAGGGAAGGTGCT GCACTATCGCATCGACAAAGACAAGACAGGGAAGCTCTCCATCCCCGAGGGAAAGAAGTTCGACACGCTCTGGCAGCTAGTCGAGCATTATTCTTATAAA GCAGATGGTTTGTTAAGAGTTCTTACTGTCCCATGTCAAAAAATCGGCACACAGGGAAATGTTAATTTTGGAGGCCGTCCACAACTTCCAGGTTCCCATC CTGCGTCCTCCCCTGCCCAAGGGAACCGGCAAGAGAGTACTGTGTCATTCAATCCGTATGAGCCAGAACTTGCACCCTGGGCTGCAGACAAAGGCCCCCA GAGAGAAGCCCTACCCATGGACACAGAGGTGTACGAGAGCCCCTACGCGGACCCCGAGGAGATCAGGCCCAAGGAGGTTTACCTGGACCGAAAGCTGCTG ACGCTGGAAGACAAAGAACTGGGCTCTGGTAATTTTGGAACTGTGAAAAAGGGCTACTACCAAATGAAAAAAGTTGTGAAAACCGTGGCTGTGAAAATAC TGAAAAACGAGGCCAATGACCCCGCTCTTAAAGATGAGTTATTAGCAGAAGCAAATGTCATGCAGCAGCTGGACAACCCGTACATCGTGCGGATGATCGG GATATGCGAGGCCGAGTCCTGGATGCTGGTTATGGAGATGGCAGAACTTGGTCCCCTCAATAAGTATTTGCAGCAGAACAGACATGTCAAGGATAAGAAC ATCATAGAACTGGTTCATCAGGTTTCCATGGGCATGAAGTACTTGGAGGAGAGCAATTTTGTGCACAGAGATCTGGCTGCAAGAAATGTGTTGCTAGTTA CCCAACATTACGCCAAGATCAGTGATTTCGGACTTTCCAAAGCACTGCGTGCTGATGAAAACTACTACAAGGCCCAGACCCATGGAAAGTGGCCTGTCAA GTGGTACGCTCCGGAATGCATCAACTACTACAAGTTCTCCAGCAAAAGCGATGTCTGGAGCTTTGGAGTGTTGATGTGGGAAGCATTCTCCTATGGGCAG AAGCCATATCGAGGGATGAAAGGAAGTGAAGTCACCGCTATGTTAGAGAAAGGAGAGCGGATGGGGTGCCCTGCAGGGTGTCCAAGAGAGATGTACGATC TCATGAATCTGTGCTGGACATACGATGTGGAAAACAGGCCCGGATTCGCAGCAGTGGAACTGCGGCTGCGCAATTACTACTATGACGTGGTGAACTAACC GCTCCCGCACCTGTCGGTGGCTGCCTTTGATCACAGGAGCAATCACAGGAAAATGTATCCAGAGGAATTGATTGTCAGCCACCTCCCTCTGCCAGTCGGG AGAGCCAGGCTTGGATGGAACATGCCCACAACTTGTCACCCAAAGCCTGTCCCAGGACTCACCCTCCACAAAGCAAAGGCAGTCCCGGGAGAAAAGACGG ATGGCAGGATCCAAGGGGCTAGCTGGATTTGTTTGTTTTCTTGTCTGTGTGATTTTCATACAGGTTATTTTTACGATCTGTTTCCAAATCCCTTTCATGT CTTTCCACTTCTCTGGGTCCCGGGGTGCATTTGTTACTCATCGGGCCCAGGGACATTGCAGAGTGGCCTAGAGCACTCTCACCCCAAGCGGCCTTTTCCA AATGCCCAAGGATGCCTTAGCATGTGACTCCTGAAGGGAAGGCAAAGGCAGAGGAATTTGGCTGCTTCTACGGCCATGAGACTGATCCCTGGCCACTGAA AAGCTTTCCTGACAATAAAAATGTTTTGAGGCTTTAAAAAGAAAATCAAGTTTGACCAGTGCAGTTTCTAAGCATGTAGCCAGTTAAGGAAAGAAAGAAA GAAAAAAAAAAAAGGCCTGGATACTGCTTTTGCTGTCTCTGTTATGAGATGGAAGACTTACATGTTTGTGATAAAAGGGGACCATGAGAATGAATTGGCT TGGCTTACTTTCCCCCTGAAATCCTCTCTCCTGCAGACTGTCTTGAAGACCTGGTGACTGGTAAATAAAGCCCTGCATGGAGGCTGCACAGCAGGGGCAA GAGGCCCATCCCCCAGCATCTCACTGAGGACAGCTTCAGGCTGCCTTCCTCTGAACGTGGTCCACACCTTCCTCTCCTCCACAGAGAGGGTGCCGCCAGA ATCCCCTGTCGCTTTCTGTGTCTGCAATGGGGGGCAGCACAGGGATCAAAGCCATCTAAAGAGTTTCCAAAGAAAGTATTAATTCAGAACAAGCCAAAGA CCCTGAGCCTCACCACAAACAGGCCTTTTGGAGTGTGAATTTGAGTTGAAGATACAAGATCGGAGAATGATTTTCTGGTCTTAACTAATCCTCATCTTCA TGTTTGATCTTTAAGAAGTCATCACCCATTGATTTCAGTTTTGCTGTACCTCTTGAAAGTTAAAGAGACATCTCAGCACTTTAGGAGGCCGAGGCGGGTG GATCACTTGAGGTAAGGAGTTTGAGACTAGCCTGGCCAATATGGTAAAACCCCATCTCTACTAAAAATACAAAAATTAGCCGGGCATGGTGGCATGTGTC TGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAAACGGAGGTCGCAGTGAGCCAAGATCATGCCACTGCACTCCAGCTTG GGCATCACAGCGAGACTCTGTCAAAACAAACAAACAAAAAAACAACTTAAAGAGGTAATTTAGCCATCATTCTTATGCCAGCAGATATAAATAAACTTGG ACCCATCTGGTCTTCAGCTAAACCTGAGACATTTTAAAGTGCATGGACAGCCATGGACAGCAGGCCCTCCTCTAACAGGGGATGCAAGGCATGGAGAAAG ACAATCAGTACCCAAGCTCAGCCACAGAAGACAGGAGTCACTCATATAACTTGTGTTTAGAAGTTTTTGGTAGCCACGCACACTTTCTGAAATCACACTA TCTGGTGGTTTAATCATATTTTTAAAGACAGAATCCCTGAGTGCTGAGCAGATTCTCAAAACACATTTAGAATCCCTGAAATTAGAAAGATCAATGACAA AATATCTGTCAGCCAGGCCACAAACAGGTGTAAAATTATGAAAGGAGTGGTTGGATGTGCCAAGTTTGGTAAAGTGGTGACTGCATCTGAGAAAGAGGCT GTGAGGCTGAACTCTTGGTGGCTTCCTTCTGTAACTTCCAGAGGGAGTCTTCAACACAGGCCCCGTGCTCGTAGGAATACGGTAGCACCTATGTAGGAAG TGCGTGGAGTTTTCTGTCTTCTTTCTGTGTGATTTTTGGCCTTTTTATCAGCACTTCTCCCCTCCCAGGAGCCTGGGGATGCCAAACATCCAGAATGTGA TGGGACAAGATGGGGGCAGGGGCCTCACCTCCCTGCAGAGGTCCGGCCAGGTCTCCTTGTCCCTGGACAATCTCCTGAGCCTCTCTGCTTGGTGGAGCAG GCACCTGTGTGCAGAATTCCCACTGTGGCCAGCACGAGGAAGTCTTTTCTAGTGAAAATGTGTCTTGTGGTCAGGAATAATTATCCTTTCCCCTGTAGCC ACCAAGGAGGGCAAATAGAGAAAGGTAACCTAATTGAAGGATTGGTCATGTGAAAAGGGCTACATTTGGGAAGCTGGGAAAGGCCTCCAGGCTTCTAGAG CAGCTAGCTTGGGCTGGATTCTCATACCCAGGCTGCCCCTTGGATTGTTCTACCCAAGCTTTTCCCTGGGGTCTGGGCTCACTCCATAAGGTAAGGTGCC TTTTACCTTATGGTCCTTCTTTAGCAGGTAACAAAGGAGCATCAGGGGCAGGCTGCCCTGGTGGCATCACACTGGCTAGTGAGGCCGTGAATATCTTGTC CCCCAGCAGGGCCGACAGTTTCTATCACAGAAAACAGTGTGTTCAGTGGTGAAAATCGTTGCATGCATGTTTTCATCTGAGCGTGTCCTTCTCCCATACT CCCTATCAGCCAGCCCTGCCTGTAGCTGCTGTATGGTGATTGCACTTGGACATCAGTCCAATGACTGCAAGTCGGCCTGGATTTTCACTTGCAGAGGCTA CAGCTGCATTGTCAGGTCTCCCAGCCCTGCAGAGAGCTCCCTCCACTGGTTAGCAGTGTGTTGTGTTTTCCATTCATTTCAGAAGAGCTACATTGTGTCA CTGGACATTTTTAAAAACTGTGATTTTTAATAAAAATTTAAAATTTGCTTTGTGATGA

SYK.F1 (forward primer)
SYK.R16 (reverse primer)
Single exon skipping

Frame 0

LPKMRKSRGPGG*GHPGGGWRARRSGWPRAAPA LASPGAGGHLRRCVPSGP*SMASSGMADSANHLP FFFGNITREEAEDYLVQGGMSDGLYLLRQSRNY LGGFALSVAHGRKAHHYTIERELNGTYAIAGGR THASPADLCHYHSQESDGLVCLLKKPFNRPQGVQ PKTGPFEDLKENLIREYVKQTWNLQGQALEQAI ISQKPQLEKLIATTAHEKMPWFHGKISREESEQ IVLIGSKTNGKFLIRARDNNGSYALCLLHEGKVL HYRIDKDKTGKLSIPEGKKFDTLWQLVEHYSYK ADGLLRVLTVPCQKIGTQGNVNFGGRPQLPGSH PASSPAQGNRQESTVSFNPYEPELAPWAADKGPQ REALPMDTEVYESPYADPEEIRPKEVYLDRKLL TLEDKELGSGNFGTVKKGYYQMKKVVKTVAVKI LKNEANDPALKDELLAEANVMQQLDNPYIVRMIG ICEAESWMLVMEMAELGPLNKYLQQNRHVKDKN IIELVHQVSMGMKYLEESNFVHRDLAARNVLLV TQHYAKISDFGLSKALRADENYYKAQTHGKWPVK WYAPECINYYKFSSKSDVWSFGVLMWEAFSYGQ KPYRGMKGSEVTAMLEKGERMGCPAGCPREMYD LMNLCWTYDVENRPGFAAVELRLRNYYYDVVN*P LPHLSVAAFDHRSNHRKMYPEELIVSHLPLPVG RARLGWNMPTTCHPKPVPGLTLHKAKAVPGEKT DGRIQGASWICLFSCLCDFHTGYFYDLFPNPFHV FPLLWVPGCICYSSGPGTLQSGLEHSHPKRPFP NAQGCLSM*LLKGRQRQRNLAASTAMRLIPGH* KAFLTIKMF*GFKKKIKFDQCSF*ACSQLRKERK KKKKGLDTAFAVSVMRWKTYMFVIKGDHENELA WLTFPLKSSLLQTVLKTW*LVNKALHGGCTAGA RGPSPSISLRTASGCLPLNVVHTFLSSTERVPPE SPVAFCVCNGGQHRDQSHLKSFQRKY*FRTSQR P*ASPQTGLLECEFELKIQDRRMIFWS*LILIF MFDL*EVITH*FQFCCTS*KLKRHLSTLGGRGGW IT*GKEFETSLANMVKPHLY*KYKN*PGMVACV CSPSYSGG*GRRIA*TQETEVAVSQDHATALQL GHHSETLSKQTNKKTT*RGNLAIILMPADINKLG PIWSSAKPETF*SAWTAMDSRPSSNRGCKAWRK TISTQAQPQKTGVTHITCV*KFLVATHTF*NHT IWWFNHIFKDRIPEC*ADSQNTFRIPEIRKINDK ISVSQATNRCKIMKGVVGCAKFGKVVTASEKEA VRLNSWWLPSVTSRGSLQHRPRARRNTVAPM*E VRGVFCLLSV*FLAFLSALLPSQEPGDAKHPECD GTRWGQGPHLPAEVRPGLLVPGQSPEPLCLVEQ APVCRIPTVASTRKSFLVKMCLVVRNNYPFPCS HQGGQIEKGNLIEGLVM*KGLHLGSWERPPGF*S S*LGLDSHTQAAPWIVLPKLFPGVWAHSIR*GA FYLMVLL*QVTKEHQGQAALVASHWLVRP*ISC PPAGPTVSITENSVFSGENRCMHVFI*ACPSPIL PISQPCL*LLYGDCTWTSVQ*LQVGLDFHLQRL QLHCQVSQPCRELPPLVSSVLCFPFISEELHCV TGHF*KL*FLIKI*NLLCD

Frame 1

CPK*GRAAGPAAEATPAAAGERGGAGGPALRPP SPHLAQVDTCAGVCPPAPEAWPAAAWLTAPTTC PSFSATSPGRRQKITWSRGA*VMGFICCARAATT WVASPCPWPTGGRHTTTPSSGS*MAPTPSPVAG PMPAPPTSATTTPRSLMAWSASSRSPSTGPKGC SPRLGPLRI*RKTSSGNM*SRHGTCRVRLWSRPS SVRSLSWRS*SLPQPMKKCLGSMEKSLGKNLSK LS**DQRQMESS*SEPETTTAPTPCACCTKGRC CTIASTKTRQGSSPSPRERSSTRSGS*SSIILIK QMVC*EFLLSHVKKSAHREMLILEAVHNFQVPI LRPPLPKGTGKRVLCHSIRMSQNLHPGLQTKAP REKPYPWTQRCTRAPTRTPRRSGPRRFTWTESC* RWKTKNWALVILEL*KRATTK*KKL*KPWL*KY *KTRPMTPLLKMSY*QKQMSCSSWTTRTSCG*S GYARPSPGCWLWRWQNLVPSISICSRTDMSRIRT S*NWFIRFPWA*STWRRAILCTEIWLQEMCC*L PNITPRSVISDFPKHCVLMKTTTRPRPMESGLS SGTLRNASTTTSSPAKAMSGALEC*CGKHSPMGR SHIEG*KEVKSPLC*RKESGWGALQGVQERCTI S*ICAGHTMWKTGPDSQQWNCGCAITTMTW*TN RSRTCRWLPLITGAITGKCIQRN*LSATSLCQSG EPGLDGTCPQLVTQSLSQDSPSTKQRQSREKRR MAGSKGLAGFVCFLVCVIFIQVIFTICFQIPFM SFHFSGSRGAFVTHRAQGHCRVA*STLTPSGLFQ MPKDALACDS*REGKGRGIWLLLRP*D*SLATE KLS*Q*KCFEALKRKSSLTSAVSKHVAS*GKKE RKKKKAWILLLLSLL*DGRLTCL**KGTMRMNWL GLLSP*NPLSCRLS*RPGDW*IKPCMEAAQQGQ EAHPPASH*GQLQAAFL*TWSTPSSPPQRGCRQ NPLSLSVSAMGGSTGIKAI*RVSKESINSEQAKD PEPHHKQAFWSVNLS*RYKIGE*FSGLN*SSSS CLIFKKSSPIDFSFAVPLES*RDISAL*EAEAG GSLEVRSLRLAWPIW*NPISTKNTKISRAWWHVS VVPATREAEAGESLEPRKRRSQ*AKIMPLHSSL GITARLCQNKQTKKQLKEVI*PSFLCQQI*INL DPSGLQLNLRHFKVHGQPWTAGPPLTGDARHGER QSVPKLSHRRQESLI*LVFRSFW*PRTLSEITL SGGLIIFLKTESLSAEQILKTHLESLKLERSMT KYLSARPQTGVKL*KEWLDVPSLVKW*LHLRKRL *G*TLGGFLL*LPEGVFNTGPVLVGIR*HLCRK CVEFSVFFLCDFWPFYQHFSPPRSLGMPNIQNV MGQDGGRGLTSLQRSGQVSLSLDNLLSLSAWWSR HLCAEFPLWPARGSLF**KCVLWSGIIILSPVA TKEGK*RKVT*LKDWSCEKGYIWEAGKGLQASR AASLGWILIPRLPLGLFYPSFSLGSGLTP*GKVP FTLWSFFSR*QRSIRGRLPWWHHTG**GREYLV PQQGRQFLSQKTVCSVVKIVACMFSSERVLLPY SLSASPACSCCMVIALGHQSNDCKSAWIFTCRGY SCIVRSPSPAESSLHWLAVCCVFHSFQKSYIVS LDIFKNCDF**KFKICFVM

Frame 2

AQNEEEPRARRLRPPRRRLESEEERVAPRCARP RLTWRRWTPAQVCALRPLKHGQQRHG*QRQPPA LLFRQHHPGGGRRLPGPGGHE*WALFAAPEPQL PGWLRPVRGPREEGTPLHHRAGAEWHLRHRRWQD PCQPRRPLPLPLPGV*WPGLPPQEALQPAPRGA AQDWAL*GFEGKPHQGICEADMEPAGSGSGAGH HQSEASAGEADRYHSP*KNALVPWKNLSGRI*AN CPDRIKDKWKVPDPSQRQQRLLRPVPAARREGA ALSHRQRQDREALHPRGKEVRHALAASRALFL* SRWFVKSSYCPMSKNRHTGKC*FWRPSTTSRFPS CVLPCPREPAREYCVIQSV*ARTCTLGCRQRPP ERSPTHGHRGVREPLRGPRGDQAQGGLPGPKAA DAGRQRTGLW*FWNCEKGLLPNEKSCENRGCENT EKRGQ*PRS*R*VISRSKCHAAAGQPVHRADDR DMRGRVLDAGYGDGRTWSPQ*VFAAEQTCQG*E HHRTGSSGFHGHEVLGGEQFCAQRSGCKKCVASY PTLRQDQ*FRTFQSTAC**KLLQGPDPWKVACQ VVRSGMHQLLQVLQQKRCLELWSVDVGSILLWA EAISRDERK*SHRYVRERRADGVPCRVSKRDVRS HESVLDIRCGKQARIRSSGTAAAQLLL*RGELT APAPVGGCL*SQEQSQENVSRGIDCQPPPSASR ESQAWMEHAHNLSPKACPRTHPPQSKGSPGRKDG WQDPRG*LDLFVFLSV*FSYRLFLRSVSKSLSC LSTSLGPGVHLLLIGPRDIAEWPRALSPQAAFS KCPRMP*HVTPEGKAKAEEFGCFYGHETDPWPLK SFPDNKNVLRL*KENQV*PVQFLSM*PVKERKK EKKKRPGYCFCCLCYEMEDLHVCDKRGP*E*IG LAYFPPEILSPADCLEDLVTGK*SPAWRLHSRGK RPIPQHLTEDSFRLPSSERGPHLPLLHREGAAR IPCRFLCLQWGAAQGSKPSKEFPKKVLIQNKPK TLSLTTNRPFGV*I*VEDTRSENDFLVLTNPHLH V*SLRSHHPLISVLLYLLKVKETSQHFRRPRRV DHLR*GV*D*PGQYGKTPSLLKIQKLAGHGGMC L*SQLLGRLRQENRLNPGNGGRSEPRSCHCTPAW ASQRDSVKTNKQKNNLKR*FSHHSYASRYK*TW THLVFS*T*DILKCMDSHGQQALL*QGMQGMEK DNQYPSSATEDRSHSYNLCLEVFGSHAHFLKSHY LVV*SYF*RQNP*VLSRFSKHI*NP*N*KDQ*Q NICQPGHKQV*NYERSGWMCQVW*SGDCI*ERG CEAELLVASFCNFQRESSTQAPCS*EYGSTYVGS AWSFLSSFCVIFGLFISTSPLPGAWGCQTSRM* WDKMGAGASPPCRGPARSPCPWTIS*ASLLGGA GTCVQNSHCGQHEEVFSSENVSCGQE*LSFPL*P PRRANRER*PN*RIGHVKRATFGKLGKASRLLE QLAWAGFSYPGCPLDCSTQAFPWGLGSLHKVRC LLPYGPSLAGNKGASGAGCPGGITLASEAVNILS PSRADSFYHRKQCVQW*KSLHACFHLSVSFSHT PYQPALPVAAVW*LHLDISPMTASRPGFSLAEA TAALSGLPALQRAPSTG*QCVVFSIHFRRATLCH WTFLKTVIFNKNLKFAL**

Sequence SYK.eAug05

SYK.F1 (forward primer)
SYK.R16 (reverse primer)
Single exon skipping

Frame 0

LRPPRRRLESEEERVAPRCARPRLTWRR*VWPR PLPGRDFLVRRGGYGERRAAFMPFLVLPSSAEVK VLKPCE*NTGGHLRRCVPSGP*SMASSGMADSA NHLPFFFGNITREEAEDYLVQGGMSDGLYLLRQ SRNYLGGFALSVAHGRKAHHYTIERELNGTYAIA GGRTHASPADLCHYHSQESDGLVCLLKKPFNRP QGVQPKTGPFEDLKENLIREYVKQTWNLQGQAL EQAIISQKPQLEKLIATTAHEKMPWFHGKISREE SEQIVLIGSKTNGKFLIRARDNNGSYALCLLHE GKVLHYRIDKDKTGKLSIPEGKKFDTLWQLVEH YSYKADGLLRVLTVPCQKIGTQGNVNFGGRPQLP GSHPATWSAGGIISRIKSYSFPKPGHRKSSPAQ GNRQESTVSFNPYEPELAPWAADKGPQREALPM DTEVYESPYADPEEIRPKEVYLDRKLLTLEDKEL GSGNFGTVKKGYYQMKKVVKTVAVKILKNEAND PALKDELLAEANVMQQLDNPYIVRMIGICEAES WMLVMEMAELGPLNKYLQQNRYCQVPPHIWKLSP KGQGPPLDGPSSLISLGSLPTLLCLPLLVHTQG P*QRDRET*SLDSALLALVVQRRTHLTVLSFFS SCSHSVLA***TQPQNILTWNLLSHWEEHFLFKE TF*INAFLRGDS*VLSVWLTLCSHE**LLKIPI TAN*LHPSRE*LCSSHLKLLLR*RNSLGVRQND G

Frame 1

*GHPGGGWRARRSGWPRAAPALASPGAGRCGRV PYPAGTFW*GEEVTGNDALLSCPFLFYLHRPR* KC*NHVNKIQVDTCAGVCPPAPEAWPAAAWLTAP TTCPSFSATSPGRRQKITWSRGA*VMGFICCAR AATTWVASPCPWPTGGRHTTTPSSGS*MAPTPS PVAGPMPAPPTSATTTPRSLMAWSASSRSPSTGP KGCSPRLGPLRI*RKTSSGNM*SRHGTCRVRLW SRPSSVRSLSWRS*SLPQPMKKCLGSMEKSLGK NLSKLS**DQRQMESS*SEPETTTAPTPCACCTK GRCCTIASTKTRQGSSPSPRERSSTRSGS*SSI ILIKQMVC*EFLLSHVKKSAHREMLILEAVHNF QVPILRLGQRVE*SQESNHTPSQSLATESPPLPK GTGKRVLCHSIRMSQNLHPGLQTKAPREKPYPW TQRCTRAPTRTPRRSGPRRFTWTESC*RWKTKN WALVILEL*KRATTK*KKL*KPWL*KY*KTRPMT PLLKMSY*QKQMSCSSWTTRTSCG*SGYARPSP GCWLWRWQNLVPSISICSRTGIVRCPHTSGSYP LRDRAHPWMGRAA*FPWEAFPPSCAFLYWSILRD LDRGTGRHSLWIQLC*L*LFREELI*QFCLFFL PVVIQSWPSDRLSLRIS*HGISFLTGKNTFFLK KHFK*MLF*EVTVKSYLFG*PYVLMSDDF*KYQS LLIDYIHQGSNCVLLI*SCC*DEETHLE*DRMM

Frame 2

EATPAAAGERGGAGGPALRPPSPHLAQVGVAAS PTRPGLSGKERRLRGTTRCFHALSCSTFIGRGK SAETM*IKYRWTPAQVCALRPLKHGQQRHG*QR QPPALLFRQHHPGGGRRLPGPGGHE*WALFAAPE PQLPGWLRPVRGPREEGTPLHHRAGAEWHLRHR RWQDPCQPRRPLPLPLPGV*WPGLPPQEALQPA PRGAAQDWAL*GFEGKPHQGICEADMEPAGSGSG AGHHQSEASAGEADRYHSP*KNALVPWKNLSGR I*ANCPDRIKDKWKVPDPSQRQQRLLRPVPAAR REGAALSHRQRQDREALHPRGKEVRHALAASRAL FL*SRWFVKSSYCPMSKNRHTGKC*FWRPSTTS RFPSCDLVSGWNNLKNQIILLPKAWPQKVLPCP REPAREYCVIQSV*ARTCTLGCRQRPPERSPTHG HRGVREPLRGPRGDQAQGGLPGPKAADAGRQRT GLW*FWNCEKGLLPNEKSCENRGCENTEKRGQ* PRS*R*VISRSKCHAAAGQPVHRADDRDMRGRVL DAGYGDGRTWSPQ*VFAAEQVLSGAPTHLEAIP *GTGPTPGWAEQPDFLGKPSHPPVPSFIGPYSG TLTEGQGDIVFGFSSVSSSCSEKNSFDSFVFFFF L*SFSLGLVIDSASEYLDMESPFSLGRTLSF*R NILNKCFSKR*QLSLICLADLMFS*VMTSENTN HC*LITSIKGVIVFFSSKAVVEMKKLTWSKTE*W

Sequence SYK.bAug05

AGCACTCTGGCTTGTTGATGCCTGTCTTGTGCTTTCTCTCTGTTTCAGCTGTTTTCCTTTTAAGATACTCCTCTCAGATTTGTGTGCTTCTTCCCTTCCC ACATTCTATACTGCCCAGCGCCTGGGATTCCAGATCTGCGTTTGAATCCAGGAAAGGGAGGTGGACACCTGCGCAGGTGTGTGCCCTCCGGCCCCTGAAG CATGGCCAGCAGCGGCATGGCTGACAGCGCCAACCACCTGCCCTTCTTTTTCGGCAACATCACCCGGGAGGAGGCAGAAGATTACCTGGTCCAGGGGGGC ATGAGTGATGGGCTTTATTTGCTGCGCCAGAGCCGCAACTACCTGGGTGGCTTCGCCCTGTCCGTGGCCCACGGGAGGAAGGCACACCACTACACCATCG AGCGGGAGCTGAATGGCACCTACGCCATCGCCGGTGGCAGGACCCATGCCAGCCCCGCCGACCTCTGCCACTACCACTCCCAGGAGTCTGATGGCCTGGT CTGCCTCCTCAAGAAGCCCTTCAACCGGCCCCAAGGGGTGCAGCCCAAGACTGGGCCCTTTGAGGATTTGAAGGAAAACCTCATCAGGGAATATGTGAAG CAGACATGGAACCTGCAGGGTCAGGCTCTGGAGCAGGCCATCATCAGTCAGAAGCCTCAGCTGGAGAAGCTGATCGCTACCACAGCCCATGAAAAAATGC CTTGGTTCCATGGAAAAATCTCTCGGGAAGAATCTGAGCAAATTGTCCTGATAGGATCAAAGACAAATGGAAAGTTCCTGATCCGAGCCAGAGACAACAA CGGCTCCTACGCCCTGTGCCTGCTGCACGAAGGGAAGGTGCTGCACTATCGCATCGACAAAGACAAGACAGGGAAGCTCTCCATCCCCGAGGGAAAGAAG TTCGACACGCTCTGGCAGCTAGTCGAGCATTATTCTTATAAAGCAGATGGTTTGTTAAGAGTTCTTACTGTCCCATGTCAAAAAATCGGCACACAGGGAA ATGTTAATTTTGGAGGCCGTCCACAACTTCCAGGTTCCCATCCTGCGACTTGGTCAGCGGGTGGAATAATCTCAAGAATCAAATCATACTCCTTCCCAAA GCCTGGCCACAGAAAGTCCTCCCCTGCCCAAGGGAACCGGCAAGAGAGTACTGTGTCATTCAATCCGTATGAGCCAGAACTTGCACCCTGGGCTGCAGAC AAAGGCCCCCAGAGAGAAGCCCTACCCATGGACACAGAGGTGTACGAGAGCCCCTACGCGGACCCCGAGGAGATCAGGCCCAAGGAGGTTTACCTGGACC GAAAGCTGCTGACGCTGGAAGACAAAGAACTGGGCTCTGGTAATTTTGGAACTGTGAAAAAGGGCTACTACCAAATGAAAAAAGTTGTGAAAACCGTGGC TGTGAAAATACTGAAAAACGAGGCCAATGACCCCGCTCTTAAAGATGAGTTATTAGCAGAAGCAAATGTCATGCAGCAGCTGGACAACCCGTACATCGTG CGGATGATCGGGATATGCGAGGCCGAGTCCTGGATGCTGGTTATGGAGATGGCAGAACTTGGTCCCCTCAATAAGTATTTGCAGCAGAACAGACATGTCA AGGATAAGAACATCATAGAACTGGTTCATCAGGTTTCCATGGGCATGAAGTACTTGGAGGAGAGCAATTTTGTGCACAGAGATCTGGCTGCAAGAAATGT GTTGCTAGTTACCCAACATTACGCCAAGATCAGTGATTTCGGACTTTCCAAAGCACTGCGTGCTGATGAAAACTACTACAAGGCCCAGACCCATGGAAAG TGGCCTGTCAAGTGGTACGCTCCGGAATGCATCAACTACTACAAGTTCTCCAGCAAAAGCGATGTCTGGAGCTTTGGAGTGTTGATGTGGGAAGCATTCT CCTATGGGCAGAAGCCATATCGAGGGATGAAAGGAAGTGAAGTCACCGCTATGTTAGAGAAAGGAGAGCGGATGGGGTGCCCTGCAGGGTGTCCAAGAGA GATGTACGATCTCATGAATCTGTGCTGGACATACGATGTGGAAAACAGGCCCGGATTCGCAGCAGTGGAACTGCGGCTGCGCAATTACTACTATGACGTG GTGAACTAACCGCTCCCGCACCTGTCGGTGGCTGCCTTTGATCACAGGAGCAATCACAGGAAAATGTATCCAGAGGAATTGATTGTCAGCCACCTCCCTC TGCCAGTCGGGAGAGCCAGGCTTGGATGGAACATGCCCACAACTTGTCACCCAAAGCCTGTCCCAGGACTCACCCTCCACAAAGCAAAGGCAGTCCCGGG AGAAAAGACGGATGGCAGGATCCAAGGGGCTAGCTGGATTTGTTTGTTTTCTTGTCTGTGTGATTTTCATACAGGTTATTTTTACGATCTGTTTCCAAAT CCCTTTCATGTCTTTCCACTTCTCTGGGTCCCGGGGTGCATTTGTTACTCATCGGGCCCAGGGACATTGCAGAGTGGCCTAGAGCACTCTCACCCCAAGC GGCCTTTTCCAAATGCCCAAGGATGCCTTAGCATGTGACTCCTGAAGGGAAGGCAAAGGCAGAGGAATTTGGCTGCTTCTACGGCCATGAGACTGATCCC TGGCCACTGAAAAGCTTTCCTGACAATAAAAATGTTTTGAGGCTTTAAAAAGAAAATCAAGTTTGACCAGTGCAGTTTCTAAGCATGTAGCCAGTTAAGG AAAGAAAGAAAGAAAAAAAAAAAAGGCCTGGATACTGCTTTTGCTGTCTCTGTTATGAGATGGAAGACTTACATGTTTGTGATAAAAGGGGACCATGAGA ATGAATTGGCTTGGCTTACTTTCCCCCTGAAATCCTCTCTCCTGCAGACTGTCTTGAAGACCTGGTGACTGGTAAATAAAGCCCTGCATGGAGGCTGCAC AGCAGGGGCAAGAGGCCCATCCCCCAGCATCTCACTGAGGACAGCTTCAGGCTGCCTTCCTCTGAACGTGGTCCACACCTTCCTCTCCTCCACAGAGAGG GTGCCGCCAGAATCCCCTGTCGCTTTCTGTGTCTGCAATGGGGGGCAGCACAGGGATCAAAGCCATCTAAAGAGTTTCCAAAGAAAGTATTAATTCAGAA CAAGCCAAAGACCCTGAGCCTCACCACAAACAGGCCTTTTGGAGTGTGAATTTGAGTTGAAGATACAAGATCGGAGAATGATTTTCTGGTCTTAACTAAT CCTCATCTTCATGTTTGATCTTTAAGAAGTCATCACCCATTGATTTCAGTTTTGCTGTACCTCTTGAAAGTTAAAGAGACATCTCAGCACTTTAGGAGGC CGAGGCGGGTGGATCACTTGAGGTAAGGAGTTTGAGACTAGCCTGGCCAATATGGTAAAACCCCATCTCTACTAAAAATACAAAAATTAGCCGGGCATGG TGGCATGTGTCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAAACGGAGGTCGCAGTGAGCCAAGATCATGCCACTGC ACTCCAGCTTGGGCATCACAGCGAGACTCTGTCAAAACAAACAAACAAAAAAACAACTTAAAGAGGTAATTTAGCCATCATTCTTATGCCAGCAGATATA AATAAACTTGGACCCATCTGGTCTTCAGCTAAACCTGAGACATTTTAAAGTGCATGGACAGCCATGGACAGCAGGCCCTCCTCTAACAGGGGATGCAAGG CATGGAGAAAGACAATCAGTACCCAAGCTCAGCCACAGAAGACAGGAGTCACTCATATAACTTGTGTTTAGAAGTTTTTGGTAGCCACGCACACTTTCTG AAATCACACTATCTGGTGGTTTAATCATATTTTTAAAGACAGAATCCCTGAGTGCTGAGCAGATTCTCAAAACACATTTAGAATCCCTGAAATTAGAAAG ATCAATGACAAAATATCTGTCAGCCAGGCCACAAACAGGTGTAAAATTATGAAAGGAGTGGTTGGATGTGCCAAGTTTGGTAAAGTGGTGACTGCATCTG AGAAAGAGGCTGTGAGGCTGAACTCTTGGTGGCTTCCTTCTGTAACTTCCAGAGGGAGTCTTCAACACAGGCCCCGTGCTCGTAGGAATACGGTAGCACC TATGTAGGAAGTGCGTGGAGTTTTCTGTCTTCTTTCTGTGTGATTTTTGGCCTTTTTATCAGCACTTCTCCCCTCCCAGGAGCCTGGGGATGCCAAACAT CCAGAATGTGATGGGACAAGATGGGGGCAGGGGCCTCACCTCCCTGCAGAGGTCCGGCCAGGTCTCCTTGTCCCTGGACAATCTCCTGAGCCTCTCTGCT TGGTGGAGCAGGCACCTGTGTGCAGAATTCCCACTGTGGCCAGCACGAGGAAGTCTTTTCTAGTGAAAATGTGTCTTGTGGTCAGGAATAATTATCCTTT CCCCTGTAGCCACCAAGGAGGGCAAATAGAGAAAGGTAACCTAATTGAAGGATTGGTCATGTGAAAAGGGCTACATTTGGGAAGCTGGGAAAGGCCTCCA GGCTTCTAGAGCAGCTAGCTTGGGCTGGATTCTCATACCCAGGCTGCCCCTTGGATTGTTCTACCCAAGCTTTTCCCTGGGGTCTGGGCTCACTCCATAA GGTAAGGTGCCTTTTACCTTATGGTCCTTCTTTAGCAGGTAACAAAGGAGCATCAGGGGCAGGCTGCCCTGGTGGCATCACACTGGCTAGTGAGGCCGTG AATATCTTGTCCCCCAGCAGGGCCGACAGTTTCTATCACAGAAAACAGTGTGTTCAGTGGTGAAAATCGTTGCATGCATGTTTTCATCTGAGCGTGTCCT TCTCCCATACTCCCTATCAGCCAGCCCTGCCTGTAGCTGCTGTATGGTGATTGCACTTGGACATCAGTCCAATGACTGCAAGTCGGCCTGGATTTTCACT TGCAGAGGCTACAGCTGCATTGTCAGGTCTCCCAGCCCTGCAGAGAGCTCCCTCCACTGGTTAGCAGTGTGTTGTGTTTTCCATTCATTTCAGAAGAGCT ACATTGTGTCACTGGACATTTTTAAAAACTGTGATTTTTAATAAAAATTTAAAATTTGCTTTGTGATGA

SYK.F1 (forward primer)
SYK.R16 (reverse primer)
Single exon skipping

Frame 0

STLAC*CLSCAFSLFQLFSF*DTPLRFVCFFPS HILYCPAPGIPDLRLNPGKGGGHLRRCVPSGP*S MASSGMADSANHLPFFFGNITREEAEDYLVQGG MSDGLYLLRQSRNYLGGFALSVAHGRKAHHYTI ERELNGTYAIAGGRTHASPADLCHYHSQESDGLV CLLKKPFNRPQGVQPKTGPFEDLKENLIREYVK QTWNLQGQALEQAIISQKPQLEKLIATTAHEKM PWFHGKISREESEQIVLIGSKTNGKFLIRARDNN GSYALCLLHEGKVLHYRIDKDKTGKLSIPEGKK FDTLWQLVEHYSYKADGLLRVLTVPCQKIGTQG NVNFGGRPQLPGSHPATWSAGGIISRIKSYSFPK PGHRKSSPAQGNRQESTVSFNPYEPELAPWAAD KGPQREALPMDTEVYESPYADPEEIRPKEVYLD RKLLTLEDKELGSGNFGTVKKGYYQMKKVVKTVA VKILKNEANDPALKDELLAEANVMQQLDNPYIV RMIGICEAESWMLVMEMAELGPLNKYLQQNRHV KDKNIIELVHQVSMGMKYLEESNFVHRDLAARNV LLVTQHYAKISDFGLSKALRADENYYKAQTHGK WPVKWYAPECINYYKFSSKSDVWSFGVLMWEAF SYGQKPYRGMKGSEVTAMLEKGERMGCPAGCPRE MYDLMNLCWTYDVENRPGFAAVELRLRNYYYDV VN*PLPHLSVAAFDHRSNHRKMYPEELIVSHLP LPVGRARLGWNMPTTCHPKPVPGLTLHKAKAVPG EKTDGRIQGASWICLFSCLCDFHTGYFYDLFPN PFHVFPLLWVPGCICYSSGPGTLQSGLEHSHPK RPFPNAQGCLSM*LLKGRQRQRNLAASTAMRLIP GH*KAFLTIKMF*GFKKKIKFDQCSF*ACSQLR KERKKKKKGLDTAFAVSVMRWKTYMFVIKGDHE NELAWLTFPLKSSLLQTVLKTW*LVNKALHGGCT AGARGPSPSISLRTASGCLPLNVVHTFLSSTER VPPESPVAFCVCNGGQHRDQSHLKSFQRKY*FR TSQRP*ASPQTGLLECEFELKIQDRRMIFWS*LI LIFMFDL*EVITH*FQFCCTS*KLKRHLSTLGG RGGWIT*GKEFETSLANMVKPHLY*KYKN*PGM VACVCSPSYSGG*GRRIA*TQETEVAVSQDHATA LQLGHHSETLSKQTNKKTT*RGNLAIILMPADI NKLGPIWSSAKPETF*SAWTAMDSRPSSNRGCK AWRKTISTQAQPQKTGVTHITCV*KFLVATHTF* NHTIWWFNHIFKDRIPEC*ADSQNTFRIPEIRK INDKISVSQATNRCKIMKGVVGCAKFGKVVTAS EKEAVRLNSWWLPSVTSRGSLQHRPRARRNTVAP M*EVRGVFCLLSV*FLAFLSALLPSQEPGDAKH PECDGTRWGQGPHLPAEVRPGLLVPGQSPEPLC LVEQAPVCRIPTVASTRKSFLVKMCLVVRNNYPF PCSHQGGQIEKGNLIEGLVM*KGLHLGSWERPP GF*SS*LGLDSHTQAAPWIVLPKLFPGVWAHSI R*GAFYLMVLL*QVTKEHQGQAALVASHWLVRP* ISCPPAGPTVSITENSVFSGENRCMHVFI*ACP SPILPISQPCL*LLYGDCTWTSVQ*LQVGLDFH LQRLQLHCQVSQPCRELPPLVSSVLCFPFISEEL HCVTGHF*KL*FLIKI*NLLCD

Frame 1

ALWLVDACLVLSLCFSCFPFKILLSDLCASSLP TFYTAQRLGFQICV*IQEREVDTCAGVCPPAPE AWPAAAWLTAPTTCPSFSATSPGRRQKITWSRGA *VMGFICCARAATTWVASPCPWPTGGRHTTTPS SGS*MAPTPSPVAGPMPAPPTSATTTPRSLMAW SASSRSPSTGPKGCSPRLGPLRI*RKTSSGNM*S RHGTCRVRLWSRPSSVRSLSWRS*SLPQPMKKC LGSMEKSLGKNLSKLS**DQRQMESS*SEPETT TAPTPCACCTKGRCCTIASTKTRQGSSPSPRERS STRSGS*SSIILIKQMVC*EFLLSHVKKSAHRE MLILEAVHNFQVPILRLGQRVE*SQESNHTPSQ SLATESPPLPKGTGKRVLCHSIRMSQNLHPGLQT KAPREKPYPWTQRCTRAPTRTPRRSGPRRFTWT ESC*RWKTKNWALVILEL*KRATTK*KKL*KPW L*KY*KTRPMTPLLKMSY*QKQMSCSSWTTRTSC G*SGYARPSPGCWLWRWQNLVPSISICSRTDMS RIRTS*NWFIRFPWA*STWRRAILCTEIWLQEM CC*LPNITPRSVISDFPKHCVLMKTTTRPRPMES GLSSGTLRNASTTTSSPAKAMSGALEC*CGKHS PMGRSHIEG*KEVKSPLC*RKESGWGALQGVQE RCTIS*ICAGHTMWKTGPDSQQWNCGCAITTMTW *TNRSRTCRWLPLITGAITGKCIQRN*LSATSL CQSGEPGLDGTCPQLVTQSLSQDSPSTKQRQSR EKRRMAGSKGLAGFVCFLVCVIFIQVIFTICFQI PFMSFHFSGSRGAFVTHRAQGHCRVA*STLTPS GLFQMPKDALACDS*REGKGRGIWLLLRP*D*S LATEKLS*Q*KCFEALKRKSSLTSAVSKHVAS*G KKERKKKKAWILLLLSLL*DGRLTCL**KGTMR MNWLGLLSP*NPLSCRLS*RPGDW*IKPCMEAA QQGQEAHPPASH*GQLQAAFL*TWSTPSSPPQRG CRQNPLSLSVSAMGGSTGIKAI*RVSKESINSE QAKDPEPHHKQAFWSVNLS*RYKIGE*FSGLN* SSSSCLIFKKSSPIDFSFAVPLES*RDISAL*EA EAGGSLEVRSLRLAWPIW*NPISTKNTKISRAW WHVSVVPATREAEAGESLEPRKRRSQ*AKIMPL HSSLGITARLCQNKQTKKQLKEVI*PSFLCQQI* INLDPSGLQLNLRHFKVHGQPWTAGPPLTGDAR HGERQSVPKLSHRRQESLI*LVFRSFW*PRTLS EITLSGGLIIFLKTESLSAEQILKTHLESLKLER SMTKYLSARPQTGVKL*KEWLDVPSLVKW*LHL RKRL*G*TLGGFLL*LPEGVFNTGPVLVGIR*H LCRKCVEFSVFFLCDFWPFYQHFSPPRSLGMPNI QNVMGQDGGRGLTSLQRSGQVSLSLDNLLSLSA WWSRHLCAEFPLWPARGSLF**KCVLWSGIIIL SPVATKEGK*RKVT*LKDWSCEKGYIWEAGKGLQ ASRAASLGWILIPRLPLGLFYPSFSLGSGLTP* GKVPFTLWSFFSR*QRSIRGRLPWWHHTG**GR EYLVPQQGRQFLSQKTVCSVVKIVACMFSSERVL LPYSLSASPACSCCMVIALGHQSNDCKSAWIFT CRGYSCIVRSPSPAESSLHWLAVCCVFHSFQKS YIVSLDIFKNCDF**KFKICFVM

Frame 2

HSGLLMPVLCFLSVSAVFLLRYSSQICVLLPFP HSILPSAWDSRSAFESRKGRWTPAQVCALRPLK HGQQRHG*QRQPPALLFRQHHPGGGRRLPGPGG HE*WALFAAPEPQLPGWLRPVRGPREEGTPLHHR AGAEWHLRHRRWQDPCQPRRPLPLPLPGV*WPG LPPQEALQPAPRGAAQDWAL*GFEGKPHQGICE ADMEPAGSGSGAGHHQSEASAGEADRYHSP*KNA LVPWKNLSGRI*ANCPDRIKDKWKVPDPSQRQQ RLLRPVPAARREGAALSHRQRQDREALHPRGKE VRHALAASRALFL*SRWFVKSSYCPMSKNRHTGK C*FWRPSTTSRFPSCDLVSGWNNLKNQIILLPK AWPQKVLPCPREPAREYCVIQSV*ARTCTLGCR QRPPERSPTHGHRGVREPLRGPRGDQAQGGLPGP KAADAGRQRTGLW*FWNCEKGLLPNEKSCENRG CENTEKRGQ*PRS*R*VISRSKCHAAAGQPVHR ADDRDMRGRVLDAGYGDGRTWSPQ*VFAAEQTCQ G*EHHRTGSSGFHGHEVLGGEQFCAQRSGCKKC VASYPTLRQDQ*FRTFQSTAC**KLLQGPDPWK VACQVVRSGMHQLLQVLQQKRCLELWSVDVGSIL LWAEAISRDERK*SHRYVRERRADGVPCRVSKR DVRSHESVLDIRCGKQARIRSSGTAAAQLLL*R GELTAPAPVGGCL*SQEQSQENVSRGIDCQPPPS ASRESQAWMEHAHNLSPKACPRTHPPQSKGSPG RKDGWQDPRG*LDLFVFLSV*FSYRLFLRSVSK SLSCLSTSLGPGVHLLLIGPRDIAEWPRALSPQA AFSKCPRMP*HVTPEGKAKAEEFGCFYGHETDP WPLKSFPDNKNVLRL*KENQV*PVQFLSM*PVK ERKKEKKKRPGYCFCCLCYEMEDLHVCDKRGP*E *IGLAYFPPEILSPADCLEDLVTGK*SPAWRLH SRGKRPIPQHLTEDSFRLPSSERGPHLPLLHRE GAARIPCRFLCLQWGAAQGSKPSKEFPKKVLIQN KPKTLSLTTNRPFGV*I*VEDTRSENDFLVLTN PHLHV*SLRSHHPLISVLLYLLKVKETSQHFRR PRRVDHLR*GV*D*PGQYGKTPSLLKIQKLAGHG GMCL*SQLLGRLRQENRLNPGNGGRSEPRSCHC TPAWASQRDSVKTNKQKNNLKR*FSHHSYASRY K*TWTHLVFS*T*DILKCMDSHGQQALL*QGMQG MEKDNQYPSSATEDRSHSYNLCLEVFGSHAHFL KSHYLVV*SYF*RQNP*VLSRFSKHI*NP*N*K DQ*QNICQPGHKQV*NYERSGWMCQVW*SGDCI* ERGCEAELLVASFCNFQRESSTQAPCS*EYGST YVGSAWSFLSSFCVIFGLFISTSPLPGAWGCQT SRM*WDKMGAGASPPCRGPARSPCPWTIS*ASLL GGAGTCVQNSHCGQHEEVFSSENVSCGQE*LSF PL*PPRRANRER*PN*RIGHVKRATFGKLGKAS RLLEQLAWAGFSYPGCPLDCSTQAFPWGLGSLHK VRCLLPYGPSLAGNKGASGAGCPGGITLASEAV NILSPSRADSFYHRKQCVQW*KSLHACFHLSVS FSHTPYQPALPVAAVW*LHLDISPMTASRPGFSL AEATAALSGLPALQRAPSTG*QCVVFSIHFRRA TLCHWTFLKTVIFNKNLKFAL**

Sequence SYK.aAug05

TTGCCCAAAATGAGGAAGAGCCGCGGGCCCGGCGGCTGAGGCCACCCCGGCGGCGGCTGGAGAGCGAGGAGGAGCGGGTGGCCCCGCGCTGCGCCCGCCC TCGCCTCACCTGGCGCAGGTGGACACCTGCGCAGGTGTGTGCCCTCCGGCCCCTGAAGCATGGCCAGCAGCGGCATGGCTGACAGCGCCAACCACCTGCC CTTCTTTTTCGGCAACATCACCCGGGAGGAGGCAGAAGATTACCTGGTCCAGGGGGGCATGAGTGATGGGCTTTATTTGCTGCGCCAGAGCCGCAACTAC CTGGGTGGCTTCGCCCTGTCCGTGGCCCACGGGAGGAAGGCACACCACTACACCATCGAGCGGGAGCTGAATGGCACCTACGCCATCGCCGGTGGCAGGA CCCATGCCAGCCCCGCCGACCTCTGCCACTACCACTCCCAGGAGTCTGATGGCCTGGTCTGCCTCCTCAAGAAGCCCTTCAACCGGCCCCAAGGGGTGCA GCCCAAGACTGGGCCCTTTGAGGATTTGAAGGAAAACCTCATCAGGGAATATGTGAAGCAGACATGGAACCTGCAGGGTCAGGCTCTGGAGCAGGCCATC ATCAGTCAGAAGCCTCAGCTGGAGAAGCTGATCGCTACCACAGCCCATGAAAAAATGCCTTGGTTCCATGGAAAAATCTCTCGGGAAGAATCTGAGCAAA TTGTCCTGATAGGATCAAAGACAAATGGAAAGTTCCTGATCCGAGCCAGAGACAACAACGGCTCCTACGCCCTGTGCCTGCTGCACGAAGGGAAGGTGCT GCACTATCGCATCGACAAAGACAAGACAGGGAAGCTCTCCATCCCCGAGGGAAAGAAGTTCGACACGCTCTGGCAGCTAGTCGAGCATTATTCTTATAAA GCAGATGGTTTGTTAAGAGTTCTTACTGTCCCATGTCAAAAAATCGGCACACAGGGAAATGTTAATTTTGGAGGCCGTCCACAACTTCCAGGTTCCCATC CTGCGACTTGGTCAGCGGGTGGAATAATCTCAAGAATCAAATCATACTCCTTCCCAAAGCCTGGCCACAGAAAGTCCTCCCCTGCCCAAGGGAACCGGCA AGAGAGTACTGTGTCATTCAATCCGTATGAGCCAGAACTTGCACCCTGGGCTGCAGACAAAGGCCCCCAGAGAGAAGCCCTACCCATGGACACAGAGGTG TACGAGAGCCCCTACGCGGACCCCGAGGAGATCAGGCCCAAGGAGGTTTACCTGGACCGAAAGCTGCTGACGCTGGAAGACAAAGAACTGGGCTCTGGTA ATTTTGGAACTGTGAAAAAGGGCTACTACCAAATGAAAAAAGTTGTGAAAACCGTGGCTGTGAAAATACTGAAAAACGAGGCCAATGACCCCGCTCTTAA AGATGAGTTATTAGCAGAAGCAAATGTCATGCAGCAGCTGGACAACCCGTACATCGTGCGGATGATCGGGATATGCGAGGCCGAGTCCTGGATGCTGGTT ATGGAGATGGCAGAACTTGGTCCCCTCAATAAGTATTTGCAGCAGAACAGACATGTCAAGGATAAGAACATCATAGAACTGGTTCATCAGGTTTCCATGG GCATGAAGTACTTGGAGGAGAGCAATTTTGTGCACAGAGATCTGGCTGCAAGAAATGTGTTGCTAGTTACCCAACATTACGCCAAGATCAGTGATTTCGG ACTTTCCAAAGCACTGCGTGCTGATGAAAACTACTACAAGGCCCAGACCCATGGAAAGTGGCCTGTCAAGTGGTACGCTCCGGAATGCATCAACTACTAC AAGTTCTCCAGCAAAAGCGATGTCTGGAGCTTTGGAGTGTTGATGTGGGAAGCATTCTCCTATGGGCAGAAGCCATATCGAGGGATGAAAGGAAGTGAAG TCACCGCTATGTTAGAGAAAGGAGAGCGGATGGGGTGCCCTGCAGGGTGTCCAAGAGAGATGTACGATCTCATGAATCTGTGCTGGACATACGATGTGGA AAACAGGCCCGGATTCGCAGCAGTGGAACTGCGGCTGCGCAATTACTACTATGACGTGGTGAACTAACCGCTCCCGCACCTGTCGGTGGCTGCCTTTGAT CACAGGAGCAATCACAGGAAAATGTATCCAGAGGAATTGATTGTCAGCCACCTCCCTCTGCCAGTCGGGAGAGCCAGGCTTGGATGGAACATGCCCACAA CTTGTCACCCAAAGCCTGTCCCAGGACTCACCCTCCACAAAGCAAAGGCAGTCCCGGGAGAAAAGACGGATGGCAGGATCCAAGGGGCTAGCTGGATTTG TTTGTTTTCTTGTCTGTGTGATTTTCATACAGGTTATTTTTACGATCTGTTTCCAAATCCCTTTCATGTCTTTCCACTTCTCTGGGTCCCGGGGTGCATT TGTTACTCATCGGGCCCAGGGACATTGCAGAGTGGCCTAGAGCACTCTCACCCCAAGCGGCCTTTTCCAAATGCCCAAGGATGCCTTAGCATGTGACTCC TGAAGGGAAGGCAAAGGCAGAGGAATTTGGCTGCTTCTACGGCCATGAGACTGATCCCTGGCCACTGAAAAGCTTTCCTGACAATAAAAATGTTTTGAGG CTTTAAAAAGAAAATCAAGTTTGACCAGTGCAGTTTCTAAGCATGTAGCCAGTTAAGGAAAGAAAGAAAGAAAAAAAAAAAAGGCCTGGATACTGCTTTT GCTGTCTCTGTTATGAGATGGAAGACTTACATGTTTGTGATAAAAGGGGACCATGAGAATGAATTGGCTTGGCTTACTTTCCCCCTGAAATCCTCTCTCC TGCAGACTGTCTTGAAGACCTGGTGACTGGTAAATAAAGCCCTGCATGGAGGCTGCACAGCAGGGGCAAGAGGCCCATCCCCCAGCATCTCACTGAGGAC AGCTTCAGGCTGCCTTCCTCTGAACGTGGTCCACACCTTCCTCTCCTCCACAGAGAGGGTGCCGCCAGAATCCCCTGTCGCTTTCTGTGTCTGCAATGGG GGGCAGCACAGGGATCAAAGCCATCTAAAGAGTTTCCAAAGAAAGTATTAATTCAGAACAAGCCAAAGACCCTGAGCCTCACCACAAACAGGCCTTTTGG AGTGTGAATTTGAGTTGAAGATACAAGATCGGAGAATGATTTTCTGGTCTTAACTAATCCTCATCTTCATGTTTGATCTTTAAGAAGTCATCACCCATTG ATTTCAGTTTTGCTGTACCTCTTGAAAGTTAAAGAGACATCTCAGCACTTTAGGAGGCCGAGGCGGGTGGATCACTTGAGGTAAGGAGTTTGAGACTAGC CTGGCCAATATGGTAAAACCCCATCTCTACTAAAAATACAAAAATTAGCCGGGCATGGTGGCATGTGTCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCA GGAGAATCGCTTGAACCCAGGAAACGGAGGTCGCAGTGAGCCAAGATCATGCCACTGCACTCCAGCTTGGGCATCACAGCGAGACTCTGTCAAAACAAAC AAACAAAAAAACAACTTAAAGAGGTAATTTAGCCATCATTCTTATGCCAGCAGATATAAATAAACTTGGACCCATCTGGTCTTCAGCTAAACCTGAGACA TTTTAAAGTGCATGGACAGCCATGGACAGCAGGCCCTCCTCTAACAGGGGATGCAAGGCATGGAGAAAGACAATCAGTACCCAAGCTCAGCCACAGAAGA CAGGAGTCACTCATATAACTTGTGTTTAGAAGTTTTTGGTAGCCACGCACACTTTCTGAAATCACACTATCTGGTGGTTTAATCATATTTTTAAAGACAG AATCCCTGAGTGCTGAGCAGATTCTCAAAACACATTTAGAATCCCTGAAATTAGAAAGATCAATGACAAAATATCTGTCAGCCAGGCCACAAACAGGTGT AAAATTATGAAAGGAGTGGTTGGATGTGCCAAGTTTGGTAAAGTGGTGACTGCATCTGAGAAAGAGGCTGTGAGGCTGAACTCTTGGTGGCTTCCTTCTG TAACTTCCAGAGGGAGTCTTCAACACAGGCCCCGTGCTCGTAGGAATACGGTAGCACCTATGTAGGAAGTGCGTGGAGTTTTCTGTCTTCTTTCTGTGTG ATTTTTGGCCTTTTTATCAGCACTTCTCCCCTCCCAGGAGCCTGGGGATGCCAAACATCCAGAATGTGATGGGACAAGATGGGGGCAGGGGCCTCACCTC CCTGCAGAGGTCCGGCCAGGTCTCCTTGTCCCTGGACAATCTCCTGAGCCTCTCTGCTTGGTGGAGCAGGCACCTGTGTGCAGAATTCCCACTGTGGCCA GCACGAGGAAGTCTTTTCTAGTGAAAATGTGTCTTGTGGTCAGGAATAATTATCCTTTCCCCTGTAGCCACCAAGGAGGGCAAATAGAGAAAGGTAACCT AATTGAAGGATTGGTCATGTGAAAAGGGCTACATTTGGGAAGCTGGGAAAGGCCTCCAGGCTTCTAGAGCAGCTAGCTTGGGCTGGATTCTCATACCCAG GCTGCCCCTTGGATTGTTCTACCCAAGCTTTTCCCTGGGGTCTGGGCTCACTCCATAAGGTAAGGTGCCTTTTACCTTATGGTCCTTCTTTAGCAGGTAA CAAAGGAGCATCAGGGGCAGGCTGCCCTGGTGGCATCACACTGGCTAGTGAGGCCGTGAATATCTTGTCCCCCAGCAGGGCCGACAGTTTCTATCACAGA AAACAGTGTGTTCAGTGGTGAAAATCGTTGCATGCATGTTTTCATCTGAGCGTGTCCTTCTCCCATACTCCCTATCAGCCAGCCCTGCCTGTAGCTGCTG TATGGTGATTGCACTTGGACATCAGTCCAATGACTGCAAGTCGGCCTGGATTTTCACTTGCAGAGGCTACAGCTGCATTGTCAGGTCTCCCAGCCCTGCA GAGAGCTCCCTCCACTGGTTAGCAGTGTGTTGTGTTTTCCATTCATTTCAGAAGAGCTACATTGTGTCACTGGACATTTTTAAAAACTGTGATTTTTAAT AAAAATTTAAAATTTGCTTTGTGATGA

SYK.F1 (forward primer)
SYK.R16 (reverse primer)
Single exon skipping

Frame 0

LPKMRKSRGPGG*GHPGGGWRARRSGWPRAAPA LASPGAGGHLRRCVPSGP*SMASSGMADSANHLP FFFGNITREEAEDYLVQGGMSDGLYLLRQSRNY LGGFALSVAHGRKAHHYTIERELNGTYAIAGGR THASPADLCHYHSQESDGLVCLLKKPFNRPQGVQ PKTGPFEDLKENLIREYVKQTWNLQGQALEQAI ISQKPQLEKLIATTAHEKMPWFHGKISREESEQ IVLIGSKTNGKFLIRARDNNGSYALCLLHEGKVL HYRIDKDKTGKLSIPEGKKFDTLWQLVEHYSYK ADGLLRVLTVPCQKIGTQGNVNFGGRPQLPGSH PATWSAGGIISRIKSYSFPKPGHRKSSPAQGNRQ ESTVSFNPYEPELAPWAADKGPQREALPMDTEV YESPYADPEEIRPKEVYLDRKLLTLEDKELGSG NFGTVKKGYYQMKKVVKTVAVKILKNEANDPALK DELLAEANVMQQLDNPYIVRMIGICEAESWMLV MEMAELGPLNKYLQQNRHVKDKNIIELVHQVSM GMKYLEESNFVHRDLAARNVLLVTQHYAKISDFG LSKALRADENYYKAQTHGKWPVKWYAPECINYY KFSSKSDVWSFGVLMWEAFSYGQKPYRGMKGSE VTAMLEKGERMGCPAGCPREMYDLMNLCWTYDVE NRPGFAAVELRLRNYYYDVVN*PLPHLSVAAFD HRSNHRKMYPEELIVSHLPLPVGRARLGWNMPT TCHPKPVPGLTLHKAKAVPGEKTDGRIQGASWIC LFSCLCDFHTGYFYDLFPNPFHVFPLLWVPGCI CYSSGPGTLQSGLEHSHPKRPFPNAQGCLSM*L LKGRQRQRNLAASTAMRLIPGH*KAFLTIKMF*G FKKKIKFDQCSF*ACSQLRKERKKKKKGLDTAF AVSVMRWKTYMFVIKGDHENELAWLTFPLKSSL LQTVLKTW*LVNKALHGGCTAGARGPSPSISLRT ASGCLPLNVVHTFLSSTERVPPESPVAFCVCNG GQHRDQSHLKSFQRKY*FRTSQRP*ASPQTGLL ECEFELKIQDRRMIFWS*LILIFMFDL*EVITH* FQFCCTS*KLKRHLSTLGGRGGWIT*GKEFETS LANMVKPHLY*KYKN*PGMVACVCSPSYSGG*G RRIA*TQETEVAVSQDHATALQLGHHSETLSKQT NKKTT*RGNLAIILMPADINKLGPIWSSAKPET F*SAWTAMDSRPSSNRGCKAWRKTISTQAQPQK TGVTHITCV*KFLVATHTF*NHTIWWFNHIFKDR IPEC*ADSQNTFRIPEIRKINDKISVSQATNRC KIMKGVVGCAKFGKVVTASEKEAVRLNSWWLPS VTSRGSLQHRPRARRNTVAPM*EVRGVFCLLSV* FLAFLSALLPSQEPGDAKHPECDGTRWGQGPHL PAEVRPGLLVPGQSPEPLCLVEQAPVCRIPTVA STRKSFLVKMCLVVRNNYPFPCSHQGGQIEKGNL IEGLVM*KGLHLGSWERPPGF*SS*LGLDSHTQ AAPWIVLPKLFPGVWAHSIR*GAFYLMVLL*QV TKEHQGQAALVASHWLVRP*ISCPPAGPTVSITE NSVFSGENRCMHVFI*ACPSPILPISQPCL*LL YGDCTWTSVQ*LQVGLDFHLQRLQLHCQVSQPC RELPPLVSSVLCFPFISEELHCVTGHF*KL*FLI KI*NLLCD

Frame 1

CPK*GRAAGPAAEATPAAAGERGGAGGPALRPP SPHLAQVDTCAGVCPPAPEAWPAAAWLTAPTTC PSFSATSPGRRQKITWSRGA*VMGFICCARAATT WVASPCPWPTGGRHTTTPSSGS*MAPTPSPVAG PMPAPPTSATTTPRSLMAWSASSRSPSTGPKGC SPRLGPLRI*RKTSSGNM*SRHGTCRVRLWSRPS SVRSLSWRS*SLPQPMKKCLGSMEKSLGKNLSK LS**DQRQMESS*SEPETTTAPTPCACCTKGRC CTIASTKTRQGSSPSPRERSSTRSGS*SSIILIK QMVC*EFLLSHVKKSAHREMLILEAVHNFQVPI LRLGQRVE*SQESNHTPSQSLATESPPLPKGTG KRVLCHSIRMSQNLHPGLQTKAPREKPYPWTQRC TRAPTRTPRRSGPRRFTWTESC*RWKTKNWALV ILEL*KRATTK*KKL*KPWL*KY*KTRPMTPLL KMSY*QKQMSCSSWTTRTSCG*SGYARPSPGCWL WRWQNLVPSISICSRTDMSRIRTS*NWFIRFPW A*STWRRAILCTEIWLQEMCC*LPNITPRSVIS DFPKHCVLMKTTTRPRPMESGLSSGTLRNASTTT SSPAKAMSGALEC*CGKHSPMGRSHIEG*KEVK SPLC*RKESGWGALQGVQERCTIS*ICAGHTMW KTGPDSQQWNCGCAITTMTW*TNRSRTCRWLPLI TGAITGKCIQRN*LSATSLCQSGEPGLDGTCPQ LVTQSLSQDSPSTKQRQSREKRRMAGSKGLAGF VCFLVCVIFIQVIFTICFQIPFMSFHFSGSRGAF VTHRAQGHCRVA*STLTPSGLFQMPKDALACDS *REGKGRGIWLLLRP*D*SLATEKLS*Q*KCFE ALKRKSSLTSAVSKHVAS*GKKERKKKKAWILLL LSLL*DGRLTCL**KGTMRMNWLGLLSP*NPLS CRLS*RPGDW*IKPCMEAAQQGQEAHPPASH*G QLQAAFL*TWSTPSSPPQRGCRQNPLSLSVSAMG GSTGIKAI*RVSKESINSEQAKDPEPHHKQAFW SVNLS*RYKIGE*FSGLN*SSSSCLIFKKSSPI DFSFAVPLES*RDISAL*EAEAGGSLEVRSLRLA WPIW*NPISTKNTKISRAWWHVSVVPATREAEA GESLEPRKRRSQ*AKIMPLHSSLGITARLCQNK QTKKQLKEVI*PSFLCQQI*INLDPSGLQLNLRH FKVHGQPWTAGPPLTGDARHGERQSVPKLSHRR QESLI*LVFRSFW*PRTLSEITLSGGLIIFLKT ESLSAEQILKTHLESLKLERSMTKYLSARPQTGV KL*KEWLDVPSLVKW*LHLRKRL*G*TLGGFLL *LPEGVFNTGPVLVGIR*HLCRKCVEFSVFFLC DFWPFYQHFSPPRSLGMPNIQNVMGQDGGRGLTS LQRSGQVSLSLDNLLSLSAWWSRHLCAEFPLWP ARGSLF**KCVLWSGIIILSPVATKEGK*RKVT *LKDWSCEKGYIWEAGKGLQASRAASLGWILIPR LPLGLFYPSFSLGSGLTP*GKVPFTLWSFFSR* QRSIRGRLPWWHHTG**GREYLVPQQGRQFLSQ KTVCSVVKIVACMFSSERVLLPYSLSASPACSCC MVIALGHQSNDCKSAWIFTCRGYSCIVRSPSPA ESSLHWLAVCCVFHSFQKSYIVSLDIFKNCDF* *KFKICFVM

Frame 2

AQNEEEPRARRLRPPRRRLESEEERVAPRCARP RLTWRRWTPAQVCALRPLKHGQQRHG*QRQPPA LLFRQHHPGGGRRLPGPGGHE*WALFAAPEPQL PGWLRPVRGPREEGTPLHHRAGAEWHLRHRRWQD PCQPRRPLPLPLPGV*WPGLPPQEALQPAPRGA AQDWAL*GFEGKPHQGICEADMEPAGSGSGAGH HQSEASAGEADRYHSP*KNALVPWKNLSGRI*AN CPDRIKDKWKVPDPSQRQQRLLRPVPAARREGA ALSHRQRQDREALHPRGKEVRHALAASRALFL* SRWFVKSSYCPMSKNRHTGKC*FWRPSTTSRFPS CDLVSGWNNLKNQIILLPKAWPQKVLPCPREPA REYCVIQSV*ARTCTLGCRQRPPERSPTHGHRG VREPLRGPRGDQAQGGLPGPKAADAGRQRTGLW* FWNCEKGLLPNEKSCENRGCENTEKRGQ*PRS* R*VISRSKCHAAAGQPVHRADDRDMRGRVLDAG YGDGRTWSPQ*VFAAEQTCQG*EHHRTGSSGFHG HEVLGGEQFCAQRSGCKKCVASYPTLRQDQ*FR TFQSTAC**KLLQGPDPWKVACQVVRSGMHQLL QVLQQKRCLELWSVDVGSILLWAEAISRDERK*S HRYVRERRADGVPCRVSKRDVRSHESVLDIRCG KQARIRSSGTAAAQLLL*RGELTAPAPVGGCL* SQEQSQENVSRGIDCQPPPSASRESQAWMEHAHN LSPKACPRTHPPQSKGSPGRKDGWQDPRG*LDL FVFLSV*FSYRLFLRSVSKSLSCLSTSLGPGVH LLLIGPRDIAEWPRALSPQAAFSKCPRMP*HVTP EGKAKAEEFGCFYGHETDPWPLKSFPDNKNVLR L*KENQV*PVQFLSM*PVKERKKEKKKRPGYCF CCLCYEMEDLHVCDKRGP*E*IGLAYFPPEILSP ADCLEDLVTGK*SPAWRLHSRGKRPIPQHLTED SFRLPSSERGPHLPLLHREGAARIPCRFLCLQW GAAQGSKPSKEFPKKVLIQNKPKTLSLTTNRPFG V*I*VEDTRSENDFLVLTNPHLHV*SLRSHHPL ISVLLYLLKVKETSQHFRRPRRVDHLR*GV*D* PGQYGKTPSLLKIQKLAGHGGMCL*SQLLGRLRQ ENRLNPGNGGRSEPRSCHCTPAWASQRDSVKTN KQKNNLKR*FSHHSYASRYK*TWTHLVFS*T*D ILKCMDSHGQQALL*QGMQGMEKDNQYPSSATED RSHSYNLCLEVFGSHAHFLKSHYLVV*SYF*RQ NP*VLSRFSKHI*NP*N*KDQ*QNICQPGHKQV *NYERSGWMCQVW*SGDCI*ERGCEAELLVASFC NFQRESSTQAPCS*EYGSTYVGSAWSFLSSFCV IFGLFISTSPLPGAWGCQTSRM*WDKMGAGASP PCRGPARSPCPWTIS*ASLLGGAGTCVQNSHCGQ HEEVFSSENVSCGQE*LSFPL*PPRRANRER*P N*RIGHVKRATFGKLGKASRLLEQLAWAGFSYP GCPLDCSTQAFPWGLGSLHKVRCLLPYGPSLAGN KGASGAGCPGGITLASEAVNILSPSRADSFYHR KQCVQW*KSLHACFHLSVSFSHTPYQPALPVAA VW*LHLDISPMTASRPGFSLAEATAALSGLPALQ RAPSTG*QCVVFSIHFRRATLCHWTFLKTVIFN KNLKFAL**