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Sequences

Sequence POLM.fAug05

ACCCGCTTCCGTCAGCCTCACTGGGGCTTCCTTCCGTCTCGCTCGGAGTTTCCCTCTGCGTTCGCTCCGCGCTGCTGGAGGCTGTCGTCCCAATGCTCCC CAAACGGCGGCGAGCGCGGGTCGGGTCCCCTAGCGGCGATGCCGCTTCCTCCACGCCGCCCTCGACGCGCTTCCCGGGAGTCGCCATCTACCTGGTCGAG CCTCGCATGGGTCGCAGCCGCCGGGCCTTCCTCACAGGCCTGGCGCGCTCCAAAGGCTTCCGCGTCCTTGACGCCTGCAGCTCCGAAGCGACACATGTTG TGATGGAAGAGACCTCAGCAGAGGAGGCCGTCAGCTGGCAGGAGCGCAGGATGGCAGCTGCTCCCCCGGGTTGCACCCCCCCAGCTCTGCTGGACATAAG CTGGTTAACAGAGAGCCTGGGAGCTGGGCAGCCTGTACCTGTGGAGTGCCGGCACCGCCTGGAGGAGCTGCTGGAGCATGGAGTGTGTGAGGAGGTGGAG AGAGTTCGGCGCTCAGAGAGGTACCAGACCATGAAGGGCTCCAGCACCACCAGGACCTGAGCACCCCAGTCCTGCGGTCCGATGTAGATGCCCTGCAGCA GGTGGTGGAGGAAGCTGTGGGGCAGGCCCTGCCTGGGGCCACCGTCACGCTGACCGGCGGCTTCCGCAGGTAAGAAGGCCCGACGTGCCCCTCTGGCTTG GCTTCAGACCCCTCCCCAGTGAAGGGACCTGAGTCGGGGACCCTGAAGCGGGCCCACTCGTTGGAAGGGATGTGGCCTGGGGCTGGAGTGAGCAGGCCGT GTCCCTGAGTCAGAGTCAGACTGACGCCCTCAGGGGGAAGTTGCAGGGCCATGACGTGGACTTCCTCATCACCCACCCCAAGGAGGGTCAGGAGGCGGGG CTGCTGCCTAGAGTGATGTGCCGCCTGCAGGACCAGGGCCTCATCCTGTACCACCAGCACCAGCACAGCTGCTGTGAGTCCCCTACCCGCCTGGCCCAAC AGAGCCACATGGACGCTTTTGAGAGAAGTTTCTGCATTTTCCGCCTACCACAACCTCCAGGGGCTGCTGTGGGGGGATCCACGAGGCCCTGCCCATCCTG GAAGGCCGTGAGAGTGGACTTGGTAGTTGCACCCGTCAGCCAGTTCCCTTTCGCCCTGCTCGGTTGGACTGGCTCCAAGCTTTTCCAGCGGGAGCTGCGC CGCTTCAGCCGGAAGGAGAAGGGCCTGTGGCTGAACAGCCATGGGCTGTTTGACCCGGAGCAGGGAAGCAGCAGTGGCAAGACTCCTAGGTCACGGAAGT CCTGCTTCTGTTGCAGAAGACATTTTTCCAAGCGGCTTCAGAGGAAGACATCTTCAGACACCTGGGCCTTGAGTACCTTCCTCCAGAGCAGAGAAACGCC TGAGCCTGCCTGTGTCCCCCACTTCCACTCAGGAAATTGGGCTGCCCCCAACCTGGCCACTGAATGTCTCCAGGCAGATATGCTGCCCCCTGACCCCCAC CTTCACCCCTCCCCGCCAAGGCCTGGCTCTTCCGGAGGTCAATTGTGCCTGCAGGATCAGTTGAGCCCCTGCTGGTGTGCTGCAGGGTGTGATGAGGTGG GAGCCCTCAGTGCCAGCCTCATCACTGTGTGACCCTGGGTCTGCTCTTAGCCTCCCCATGGCTCACGTTCCTGCCCTGGATGGGATGTGAGTGGGGCCCA CATCGTGGAGCTGTGGTGGGGCCTGCAGTCATGAATGGCAAGTGGTCCCTGATGTGCAGTGTCTCATTAGTTGCACTGCAGTTAACTGTGGCTCCTGCAG GGCACCCTGCCCAGAATGCCCAGAAGAGAACCATGCATACCTGCACTGCATTTGAGAGCCATGAGCTGGAGGCTGTGGTTCGTGCCAGCAAGGAGCCTAC TGTCTGGTGTGCTGTAGGCATCTGGAGAGGGAGAGGGCCTGGGTAGGAGCTGGGAGGAAGATAATTTTCAACTATGGGGCTTCAGTACTGCAGCGCCCCG AGCCAGGCTCTGTGCTTCTGCCTTTAAGGCCTGTTCTCAGCACAATGTCTCAAAAATAGGTCATATCCTGCCACTCCCGTCGCAGAGCCCTTTAATGGTT CCAAACCCTAAGTCCACACATAGCCCCTGGCTCTGGCATCTCTCCAGCCCCACTGGCCCCGAGCTGCTTGACTCACCGGCTTCCTATTTGATGCACCCAG GCCCCCTTGTGGCCAACTCCCTCCCCTTCTCACTGAGGCAGAAGCACTGAGGTGGGCTGGACATGGGTGCCCTCCACGTCCCTCATATCCCCAGGCACAC TCTGGCCTCAGGTTTTGCCCTGGCCATGTCATCTACCTGGAGTGGGCCCTCCCCTTCTTCAGGCCTTGAATCAAAAGCCACTTTGTTAGGCGAGGATTTC CCAGACCACTCATCACATTAAAAAATATTTTGAAAACATGCAGTAAAGTTGACTCTTGGGGTGCAGTTTTTGCACACGTAGGGCACACCAGGTCCATCCC CAGAACACTTCCCATGCTGTCCTATTGTGGTCATGTGCTTCTCCACCCCCACCCACTTAGCGGTGCTACATCCCTATAGTTTTGCTTTCTCTAGAATGTC ATGTAAATAGAACCAAACTATATTTAACCTTTGGACTGGCCTCTCCCCACAGCCTAATGCCTTTGACGTTCCTGCAGGTTGCTGGGTATGTTGAGTTTGT AATTTGTTCCATGCCATGGGTGTTCTACAG

POLM.u.f.1 (forward primer)
POLM.u.r.6 (reverse primer)
Multiple exon skipping

Frame 0

TRFRQPHWGFLPSRSEFPSAFAPRCWRLSSQCS PNGGERGSGPLAAMPLPPRRPRRASRESPSTWSS LAWVAAAGPSSQAWRAPKASASLTPAAPKRHML *WKRPQQRRPSAGRSAGWQLLPRVAPPQLCWT* AG*QRAWELGSLYLWSAGTAWRSCWSMECVRRWR EFGAQRGTRP*RAPAPPGPEHPSPAVRCRCPAA GGGGSCGAGPAWGHRHADRRLPQVRRPDVPLWL GFRPLPSEGT*VGDPEAGPLVGRDVAWGWSEQAV SLSQSQTDALRGKLQGHDVDFLITHPKEGQEAG LLPRVMCRLQDQGLILYHQHQHSCCESPTRLAQ QSHMDAFERSFCIFRLPQPPGAAVGGSTRPCPSW KAVRVDLVVAPVSQFPFALLGWTGSKLFQRELR RFSRKEKGLWLNSHGLFDPEQGSSSGKTPRSRK SCFCCRRHFSKRLQRKTSSDTWALSTFLQSRETP EPACVPHFHSGNWAAPNLATECLQADMLPPDPH LHPSPPRPGSSGGQLCLQDQLSPCWCAAGCDEV GALSASLITV*PWVCS*PPHGSRSCPGWDVSGAH IVELWWGLQS*MASGP*CAVSH*LHCS*LWLLQ GTLPRMPRREPCIPALHLRAMSWRLWFVPARSL LSGVL*ASGEGEGLGRSWEEDNFQLWGFSTAAPR ARLCASAFKACSQHNVSKIGHILPLPSQSPLMV PNPKSTHSPWLWHLSSPTGPELLDSPASYLMHP GPLVANSLPFSLRQKH*GGLDMGALHVPHIPRHT LASGFALAMSSTWSGPSPSSGLESKATLLGEDF PDHSSH*KIF*KHAVKLTLGVQFLHT*GTPGPS PEHFPCCPIVVMCFSTPTHLAVLHPYSFAFSRMS CK*NQTIFNLWTGLSPQPNAFDVPAGCWVC*VC NLFHAMGVLQ

Frame 1

PASVSLTGASFRLARSFPLRSLRAAGGCRPNAP QTAASAGRVP*RRCRFLHAALDALPGSRHLPGR ASHGSQPPGLPHRPGALQRLPRP*RLQLRSDTCC DGRDLSRGGRQLAGAQDGSCSPGLHPPSSAGHK LVNREPGSWAACTCGVPAPPGGAAGAWSV*GGG ESSALREVPDHEGLQHHQDLSTPVLRSDVDALQQ VVEEAVGQALPGATVTLTGGFRR*EGPTCPSGL ASDPSPVKGPESGTLKRAHSLEGMWPGAGVSRP CP*VRVRLTPSGGSCRAMTWTSSSPTPRRVRRRG CCLE*CAACRTRASSCTTSTSTAAVSPLPAWPN RATWTLLREVSAFSAYHNLQGLLWGDPRGPAHP GRP*EWTW*LHPSASSLSPCSVGLAPSFSSGSCA ASAGRRRACG*TAMGCLTRSREAAVARLLGHGS PASVAEDIFPSGFRGRHLQTPGP*VPSSRAEKR LSLPVSPTSTQEIGLPPTWPLNVSRQICCPLTPT FTPPRQGLALPEVNCACRIS*APAGVLQGVMRW EPSVPASSLCDPGSALSLPMAHVPALDGM*VGP TSWSCGGACSHEWQVVPDVQCLISCTAVNCGSCR APCPECPEENHAYLHCI*EP*AGGCGSCQQGAY CLVCCRHLERERAWVGAGRKIIFNYGASVLQRP EPGSVLLPLRPVLSTMSQK*VISCHSRRRAL*WF QTLSPHIAPGSGISPAPLAPSCLTHRLPI*CTQ APLWPTPSPSH*GRSTEVGWTWVPSTSLISPGT LWPQVLPWPCHLPGVGPPLLQALNQKPLC*ARIS QTTHHIKKYFENMQ*S*LLGCSFCTRRAHQVHP QNTSHAVLLWSCASPPPPT*RCYIPIVLLSLEC HVNRTKLYLTFGLASPHSLMPLTFLQVAGYVEFV ICSMPWVFY

Frame 2

PLPSASLGLPSVSLGVSLCVRSALLEAVVPMLP KRRRARVGSPSGDAASSTPPSTRFPGVAIYLVE PRMGRSRRAFLTGLARSKGFRVLDACSSEATHV VMEETSAEEAVSWQERRMAAAPPGCTPPALLDIS WLTESLGAGQPVPVECRHRLEELLEHGVCEEVE RVRRSERYQTMKGSSTTRT*APQSCGPM*MPCS RWWRKLWGRPCLGPPSR*PAASAGKKARRAPLAW LQTPPQ*RDLSRGP*SGPTRWKGCGLGLE*AGR VPESESD*RPQGEVAGP*RGLPHHPPQGGSGGG AAA*SDVPPAGPGPHPVPPAPAQLL*VPYPPGPT EPHGRF*EKFLHFPPTTTSRGCCGGIHEALPIL EGRESGLGSCTRQPVPFRPARLDWLQAFPAGAA PLQPEGEGPVAEQPWAV*PGAGKQQWQDS*VTEV LLLLQKTFFQAASEEDIFRHLGLEYLPPEQRNA *ACLCPPLPLRKLGCPQPGH*MSPGRYAAP*PP PSPLPAKAWLFRRSIVPAGSVEPLLVCCRV**GG SPQCQPHHCVTLGLLLASPWLTFLPWMGCEWGP HRGAVVGPAVMNGKWSLMCSVSLVALQLTVAPA GHPAQNAQKRTMHTCTAFESHELEAVVRASKEPT VWCAVGIWRGRGPG*ELGGR*FSTMGLQYCSAP SQALCFCL*GLFSAQCLKNRSYPATPVAEPFNG SKP*VHT*PLALASLQPHWPRAA*LTGFLFDAPR PPCGQLPPLLTEAEALRWAGHGCPPRPSYPQAH SGLRFCPGHVIYLEWALPFFRP*IKSHFVRRGF PRPLITLKNILKTCSKVDSWGAVFAHVGHTRSIP RTLPMLSYCGHVLLHPHPLSGATSL*FCFL*NV M*IEPNYI*PLDWPLPTA*CL*RSCRLLGMLSL *FVPCHGCST

Sequence POLM.aAug05

ACCCGCTTCCGTCAGCCTCACTGGGGCTTCCTTCCGTCTCGCTCGGAGTTTCCCTCTGCGTTCGCTCCGCGCTGCTGGAGGCTGTCGTCCCAATGCTCCC CAAACGGCGGCGAGCGCGGGTCGGGTCCCCTAGCGGCGATGCCGCTTCCTCCACGCCGCCCTCGACGCGCTTCCCGGGAGTCGCCATCTACCTGGTCGAG CCTCGCATGGGTCGCAGCCGCCGGGCCTTCCTCACAGGCCTGGCGCGCTCCAAAGGCTTCCGCGTCCTTGACGCCTGCAGCTCCGAAGCGACACATGTTG TGATGGAAGAGACCTCAGCAGAGGAGGCCGTCAGCTGGCAGGAGCGCAGGATGGCAGCTGCTCCCCCGGGTTGCACCCCCCCAGCTCTGCTGGACATAAG CTGGTTAACAGAGAGCCTGGGAGCTGGGCAGCCTGTACCTGTGGAGTGCCGGCACCGCCTGGAGGTGGCTGGGCCAAGGAAGGGGCCTCTGAGCCCAGCA TGGATGCCTGCCTATGCCTGCCAGCGCCCTACGCCCCTCACACACCACAACACTGGCCTCTCCGAGGCTCTGGAGATACTGGCCGAGGCAGCAGGCTTTG AAGGCAGTGAGGGCCGCCTCCTCACCTTCTGCAGAGCAGCCTCGGTGCTCAAGGCCCTTCCCAGCCCTGTCACAACCCTGAGCCAGCTGCAGGGGCTTCC CCACTTTGGAGAACACTCCTCTAGGGTTGTCCAGGAGCTGCTGGAGCATGGAGTGTGTGAGGAGGTGGAGAGAGTTCGGCGCTCAGAGAGGGCTCCAGCA CCACCAGGACCTGAGCACCCCAGTCCTGCGGTCCGATGTAGATGCCCTGCAGCAGGTGGTGGAGGAAGCTGTGGGGCAGGCCCTGCCTGGGGCCACCGTC ACGCTGACCGGCGGCTTCCGCAGGGCCTCATCCTGTACCACCAGCACCAGCACAGCTGCTGTGAGTCCCCTACCCGCCTGGCCCAACAGAGCCACATGGA CGCTTTTGAGAGAAGTTTCTGCATTTTCCGCCTACCACAACCTCCAGGGGCTGCTGTGGGGGGATCCACGAGGCCCTGCCCATCCTGGAAGGCCGTGAGA GTGGACTTGGTAGTTGCACCCGTCAGCCAGTTCCCTTTCGCCCTGCTCGGTTGGACTGGCTCCAAGCTTTTCCAGCGGGAGCTGCGCCGCTTCAGCCGGA AGGAGAAGGGCCTGTGGCTGAACAGCCATGGGCTGTTTGACCCGGAGCAGGGAAGCAGCAGTGGCAAGACTCCTAGGTCACGGAAGTCCTGCTTCTGTTG CAGAAGACATTTTTCCAAGCGGCTTCAGAGGAAGACATCTTCAGACACCTGGGCCTTGAGTACCTTCCTCCAGAGCAGAGAAACGCCTGAGCCTGCCTGT GTCCCCCACTTCCACTCAGGAAATTGGGCTGCCCCCAACCTGGCCACTGAATGTCTCCAGGCAGATATGCTGCCCCCTGACCCCCACCTTCACCCCTCCC CGCCAAGGCCTGGCTCTTCCGGAGGTCAATTGTGCCTGCAGGATCAGTTGAGCCCCTGCTGGTGTGCTGCAGGGTGTGATGAGGTGGGAGCCCTCAGTGC CAGCCTCATCACTGTGTGACCCTGGGTCTGCTCTTAGCCTCCCCATGGCTCACGTTCCTGCCCTGGATGGGATGTGAGTGGGGCCCACATCGTGGAGCTG TGGTGGGGCCTGCAGTCATGAATGGCAAGTGGTCCCTGATGTGCAGTGTCTCATTAGTTGCACTGCAGTTAACTGTGGCTCCTGCAGGGCACCCTGCCCA GAATGCCCAGAAGAGAACCATGCATACCTGCACTGCATTTGAGAGCCATGAGCTGGAGGCTGTGGTTCGTGCCAGCAAGGAGCCTACTGTCTGGTGTGCT GTAGGCATCTGGAGAGGGAGAGGGCCTGGGTAGGAGCTGGGAGGAAGATAATTTTCAACTATGGGGCTTCAGTACTGCAGCGCCCCGAGCCAGGCTCTGT GCTTCTGCCTTTAAGGCCTGTTCTCAGCACAATGTCTCAAAAATAGGTCATATCCTGCCACTCCCGTCGCAGAGCCCTTTAATGGTTCCAAACCCTAAGT CCACACATAGCCCCTGGCTCTGGCATCTCTCCAGCCCCACTGGCCCCGAGCTGCTTGACTCACCGGCTTCCTATTTGATGCACCCAGGCCCCCTTGTGGC CAACTCCCTCCCCTTCTCACTGAGGCAGAAGCACTGAGGTGGGCTGGACATGGGTGCCCTCCACGTCCCTCATATCCCCAGGCACACTCTGGCCTCAGGT TTTGCCCTGGCCATGTCATCTACCTGGAGTGGGCCCTCCCCTTCTTCAGGCCTTGAATCAAAAGCCACTTTGTTAGGCGAGGATTTCCCAGACCACTCAT CACATTAAAAAATATTTTGAAAACATGCAGTAAAGTTGACTCTTGGGGTGCAGTTTTTGCACACGTAGGGCACACCAGGTCCATCCCCAGAACACTTCCC ATGCTGTCCTATTGTGGTCATGTGCTTCTCCACCCCCACCCACTTAGCGGTGCTACATCCCTATAGTTTTGCTTTCTCTAGAATGTCATGTAAATAGAAC CAAACTATATTTAACCTTTGGACTGGCCTCTCCCCACAGCCTAATGCCTTTGACGTTCCTGCAGGTTGCTGGGTATGTTGAGTTTGTAATTTGTTCCATG CCATGGGTGTTCTACAG

POLM.u.f.1 (forward primer)
POLM.u.r.6 (reverse primer)
Multiple exon skipping

Frame 0

TRFRQPHWGFLPSRSEFPSAFAPRCWRLSSQCS PNGGERGSGPLAAMPLPPRRPRRASRESPSTWSS LAWVAAAGPSSQAWRAPKASASLTPAAPKRHML *WKRPQQRRPSAGRSAGWQLLPRVAPPQLCWT* AG*QRAWELGSLYLWSAGTAWRWLGQGRGL*AQH GCLPMPASALRPSHTTTLASPRLWRYWPRQQAL KAVRAASSPSAEQPRCSRPFPALSQP*ASCRGF PTLENTPLGLSRSCWSMECVRRWREFGAQRGLQH HQDLSTPVLRSDVDALQQVVEEAVGQALPGATV TLTGGFRRASSCTTSTSTAAVSPLPAWPNRATW TLLREVSAFSAYHNLQGLLWGDPRGPAHPGRP*E WTW*LHPSASSLSPCSVGLAPSFSSGSCAASAG RRRACG*TAMGCLTRSREAAVARLLGHGSPASV AEDIFPSGFRGRHLQTPGP*VPSSRAEKRLSLPV SPTSTQEIGLPPTWPLNVSRQICCPLTPTFTPP RQGLALPEVNCACRIS*APAGVLQGVMRWEPSV PASSLCDPGSALSLPMAHVPALDGM*VGPTSWSC GGACSHEWQVVPDVQCLISCTAVNCGSCRAPCP ECPEENHAYLHCI*EP*AGGCGSCQQGAYCLVC CRHLERERAWVGAGRKIIFNYGASVLQRPEPGSV LLPLRPVLSTMSQK*VISCHSRRRAL*WFQTLS PHIAPGSGISPAPLAPSCLTHRLPI*CTQAPLW PTPSPSH*GRSTEVGWTWVPSTSLISPGTLWPQV LPWPCHLPGVGPPLLQALNQKPLC*ARISQTTH HIKKYFENMQ*S*LLGCSFCTRRAHQVHPQNTS HAVLLWSCASPPPPT*RCYIPIVLLSLECHVNRT KLYLTFGLASPHSLMPLTFLQVAGYVEFVICSM PWVFY

Frame 1

PASVSLTGASFRLARSFPLRSLRAAGGCRPNAP QTAASAGRVP*RRCRFLHAALDALPGSRHLPGR ASHGSQPPGLPHRPGALQRLPRP*RLQLRSDTCC DGRDLSRGGRQLAGAQDGSCSPGLHPPSSAGHK LVNREPGSWAACTCGVPAPPGGGWAKEGASEPS MDACLCLPAPYAPHTPQHWPLRGSGDTGRGSRL* RQ*GPPPHLLQSSLGAQGPSQPCHNPEPAAGAS PLWRTLL*GCPGAAGAWSV*GGGESSALREGSS TTRT*APQSCGPM*MPCSRWWRKLWGRPCLGPPS R*PAASAGPHPVPPAPAQLL*VPYPPGPTEPHG RF*EKFLHFPPTTTSRGCCGGIHEALPILEGRE SGLGSCTRQPVPFRPARLDWLQAFPAGAAPLQPE GEGPVAEQPWAV*PGAGKQQWQDS*VTEVLLLL QKTFFQAASEEDIFRHLGLEYLPPEQRNA*ACL CPPLPLRKLGCPQPGH*MSPGRYAAP*PPPSPLP AKAWLFRRSIVPAGSVEPLLVCCRV**GGSPQC QPHHCVTLGLLLASPWLTFLPWMGCEWGPHRGA VVGPAVMNGKWSLMCSVSLVALQLTVAPAGHPAQ NAQKRTMHTCTAFESHELEAVVRASKEPTVWCA VGIWRGRGPG*ELGGR*FSTMGLQYCSAPSQAL CFCL*GLFSAQCLKNRSYPATPVAEPFNGSKP*V HT*PLALASLQPHWPRAA*LTGFLFDAPRPPCG QLPPLLTEAEALRWAGHGCPPRPSYPQAHSGLR FCPGHVIYLEWALPFFRP*IKSHFVRRGFPRPLI TLKNILKTCSKVDSWGAVFAHVGHTRSIPRTLP MLSYCGHVLLHPHPLSGATSL*FCFL*NVM*IE PNYI*PLDWPLPTA*CL*RSCRLLGMLSL*FVPC HGCST

Frame 2

PLPSASLGLPSVSLGVSLCVRSALLEAVVPMLP KRRRARVGSPSGDAASSTPPSTRFPGVAIYLVE PRMGRSRRAFLTGLARSKGFRVLDACSSEATHV VMEETSAEEAVSWQERRMAAAPPGCTPPALLDIS WLTESLGAGQPVPVECRHRLEVAGPRKGPLSPA WMPAYACQRPTPLTHHNTGLSEALEILAEAAGF EGSEGRLLTFCRAASVLKALPSPVTTLSQLQGLP HFGEHSSRVVQELLEHGVCEEVERVRRSERAPA PPGPEHPSPAVRCRCPAAGGGGSCGAGPAWGHR HADRRLPQGLILYHQHQHSCCESPTRLAQQSHMD AFERSFCIFRLPQPPGAAVGGSTRPCPSWKAVR VDLVVAPVSQFPFALLGWTGSKLFQRELRRFSR KEKGLWLNSHGLFDPEQGSSSGKTPRSRKSCFCC RRHFSKRLQRKTSSDTWALSTFLQSRETPEPAC VPHFHSGNWAAPNLATECLQADMLPPDPHLHPS PPRPGSSGGQLCLQDQLSPCWCAAGCDEVGALSA SLITV*PWVCS*PPHGSRSCPGWDVSGAHIVEL WWGLQS*MASGP*CAVSH*LHCS*LWLLQGTLP RMPRREPCIPALHLRAMSWRLWFVPARSLLSGVL *ASGEGEGLGRSWEEDNFQLWGFSTAAPRARLC ASAFKACSQHNVSKIGHILPLPSQSPLMVPNPK STHSPWLWHLSSPTGPELLDSPASYLMHPGPLVA NSLPFSLRQKH*GGLDMGALHVPHIPRHTLASG FALAMSSTWSGPSPSSGLESKATLLGEDFPDHS SH*KIF*KHAVKLTLGVQFLHT*GTPGPSPEHFP CCPIVVMCFSTPTHLAVLHPYSFAFSRMSCK*N QTIFNLWTGLSPQPNAFDVPAGCWVC*VCNLFH AMGVLQ

Sequence POLM.bAug05

ACCCGCTTCCGTCAGCCTCACTGGGGCTTCCTTCCGTCTCGCTCGGAGTTTCCCTCTGCGTTCGCTCCGCGCTGCTGGAGGCTGTCGTCCCAATGCTCCC CAAACGGCGGCGAGCGCGGGTCGGGTCCCCTAGCGGCGATGCCGCTTCCTCCACGCCGCCCTCGACGCGCTTCCCGGGAGTCGCCATCTACCTGGTCGAG CCTCGCATGGGTCGCAGCCGCCGGGCCTTCCTCACAGGCCTGGCGCGCTCCAAAGGCTTCCGCGTCCTTGACGCCTGCAGCTCCGAAGCGACACATGTTG TGATGGAAGAGACCTCAGCAGAGGAGGCCGTCAGCTGGCAGGAGCGCAGGATGGCAGCTGCTCCCCCGGGTTGCACCCCCCCAGCTCTGCTGGACATAAG CTGGTTAACAGAGAGCCTGGGAGCTGGGCAGCCTGTACCTGTGGAGTGCCGGCACCGCCTGGAGGTGGCTGGGCCAAGGAAGGGGCCTCTGAGCCCAGCA TGGATGCCTGCCTATGCCTGCCAGCGCCCTACGCCCCTCACACACCACAACACTGGCCTCTCCGAGGCTCTGGAGATACTGGCCGAGGCAGCAGGCTTTG AAGGCAGTGAGGGCCGCCTCCTCACCTTCTGCAGAGCAGCCTCGGTGCTCAAGGCCCTTCCCAGCCCTGTCACAACCCTGAGCCAGCTGCAGGGGCTTCC CCACTTTGGAGAACACTCCTCTAGGGTTGTCCAGGAGCTGCTGGAGCATGGAGTGTGTGAGGAGGTGGAGAGAGTTCGGCGCTCAGAGAGGTACCAGACC ATGAAGCTCTTCACCCAGATCTTCGGGGTCGGTGTGAAGACTGCTGACCGGTGGTACCGGGAAGGACTGCGAACCTTAGATGACCTCCGAGAGCAGCCCC AGAAACTAACCCAACAGCAGAAAGCGGGGCTCCAGCACCACCAGGACCTGAGCACCCCAGTCCTGCGGTCCGATGTAGATGCCCTGCAGCAGGTGGTGGA GGAAGCTGTGGGGCAGGCCCTGCCTGGGGCCACCGTCACGCTGACCGGCGGCTTCCGCAGGGGGAAGTTGCAGGGCCATGACGTGGACTTCCTCATCACC CACCCCAAGGAGGGTCAGGAGGCGGGGCTGCTGCCTAGAGTGATGTGCCGCCTGCAGGACCAGGGCCTCATCCTGTACCACCAGCACCAGCACAGCTGCT GTGAGTCCCCTACCCGCCTGGCCCAACAGAGCCACATGGACGCTTTTGAGAGAAGTTTCTGCATTTTCCGCCTACCACAACCTCCAGGGGCTGCTGTGGG GGGATCCACGAGGCCCTGCCCATCCTGGAAGGCCGTGAGAGTGGACTTGGTAGTTGCACCCGTCAGCCAGTTCCCTTTCGCCCTGCTCGGTTGGACTGGC TCCAAGCTTTTCCAGCGGGAGCTGCGCCGCTTCAGCCGGAAGGAGAAGGGCCTGTGGCTGAACAGCCATGGGCTGTTTGACCCGGAGCAGAAGACATTTT TCCAAGCGGCTTCAGAGGAAGACATCTTCAGACACCTGGGCCTTGAGTACCTTCCTCCAGAGCAGAGAAACGCCTGAGCCTGCCTGTGTCCCCCACTTCC ACTCAGGAAATTGGGCTGCCCCCAACCTGGCCACTGAATGTCTCCAGGCAGATATGCTGCCCCCTGACCCCCACCTTCACCCCTCCCCGCCAAGGCCTGG CTCTTCCGGAGGTCAATTGTGCCTGCAGGATCAGTTGAGCCCCTGCTGGTGTGCTGCAGGGTGTGATGAGGTGGGAGCCCTCAGTGCCAGCCTCATCACT GTGTGACCCTGGGTCTGCTCTTAGCCTCCCCATGGCTCACGTTCCTGCCCTGGATGGGATGTGAGTGGGGCCCACATCGTGGAGCTGTGGTGGGGCCTGC AGTCATGAATGGCAAGTGGTCCCTGATGTGCAGTGTCTCATTAGTTGCACTGCAGTTAACTGTGGCTCCTGCAGGGCACCCTGCCCAGAATGCCCAGAAG AGAACCATGCATACCTGCACTGCATTTGAGAGCCATGAGCTGGAGGCTGTGGTTCGTGCCAGCAAGGAGCCTACTGTCTGGTGTGCTGTAGGCATCTGGA GAGGGAGAGGGCCTGGGTAGGAGCTGGGAGGAAGATAATTTTCAACTATGGGGCTTCAGTACTGCAGCGCCCCGAGCCAGGCTCTGTGCTTCTGCCTTTA AGGCCTGTTCTCAGCACAATGTCTCAAAAATAGGTCATATCCTGCCACTCCCGTCGCAGAGCCCTTTAATGGTTCCAAACCCTAAGTCCACACATAGCCC CTGGCTCTGGCATCTCTCCAGCCCCACTGGCCCCGAGCTGCTTGACTCACCGGCTTCCTATTTGATGCACCCAGGCCCCCTTGTGGCCAACTCCCTCCCC TTCTCACTGAGGCAGAAGCACTGAGGTGGGCTGGACATGGGTGCCCTCCACGTCCCTCATATCCCCAGGCACACTCTGGCCTCAGGTTTTGCCCTGGCCA TGTCATCTACCTGGAGTGGGCCCTCCCCTTCTTCAGGCCTTGAATCAAAAGCCACTTTGTTAGGCGAGGATTTCCCAGACCACTCATCACATTAAAAAAT ATTTTGAAAACATGCAGTAAAGTTGACTCTTGGGGTGCAGTTTTTGCACACGTAGGGCACACCAGGTCCATCCCCAGAACACTTCCCATGCTGTCCTATT GTGGTCATGTGCTTCTCCACCCCCACCCACTTAGCGGTGCTACATCCCTATAGTTTTGCTTTCTCTAGAATGTCATGTAAATAGAACCAAACTATATTTA ACCTTTGGACTGGCCTCTCCCCACAGCCTAATGCCTTTGACGTTCCTGCAGGTTGCTGGGTATGTTGAGTTTGTAATTTGTTCCATGCCATGGGTGTTCT ACAG

POLM.u.f.1 (forward primer)
POLM.u.r.6 (reverse primer)
Multiple exon skipping

Frame 0

TRFRQPHWGFLPSRSEFPSAFAPRCWRLSSQCS PNGGERGSGPLAAMPLPPRRPRRASRESPSTWSS LAWVAAAGPSSQAWRAPKASASLTPAAPKRHML *WKRPQQRRPSAGRSAGWQLLPRVAPPQLCWT* AG*QRAWELGSLYLWSAGTAWRWLGQGRGL*AQH GCLPMPASALRPSHTTTLASPRLWRYWPRQQAL KAVRAASSPSAEQPRCSRPFPALSQP*ASCRGF PTLENTPLGLSRSCWSMECVRRWREFGAQRGTRP *SSSPRSSGSV*RLLTGGTGKDCEP*MTSESSP RN*PNSRKRGSSTTRT*APQSCGPM*MPCSRWW RKLWGRPCLGPPSR*PAASAGGSCRAMTWTSSSP TPRRVRRRGCCLE*CAACRTRASSCTTSTSTAA VSPLPAWPNRATWTLLREVSAFSAYHNLQGLLW GDPRGPAHPGRP*EWTW*LHPSASSLSPCSVGLA PSFSSGSCAASAGRRRACG*TAMGCLTRSRRHF SKRLQRKTSSDTWALSTFLQSRETPEPACVPHF HSGNWAAPNLATECLQADMLPPDPHLHPSPPRPG SSGGQLCLQDQLSPCWCAAGCDEVGALSASLIT V*PWVCS*PPHGSRSCPGWDVSGAHIVELWWGL QS*MASGP*CAVSH*LHCS*LWLLQGTLPRMPRR EPCIPALHLRAMSWRLWFVPARSLLSGVL*ASG EGEGLGRSWEEDNFQLWGFSTAAPRARLCASAF KACSQHNVSKIGHILPLPSQSPLMVPNPKSTHSP WLWHLSSPTGPELLDSPASYLMHPGPLVANSLP FSLRQKH*GGLDMGALHVPHIPRHTLASGFALA MSSTWSGPSPSSGLESKATLLGEDFPDHSSH*KI F*KHAVKLTLGVQFLHT*GTPGPSPEHFPCCPI VVMCFSTPTHLAVLHPYSFAFSRMSCK*NQTIF NLWTGLSPQPNAFDVPAGCWVC*VCNLFHAMGVL Q

Frame 1

PASVSLTGASFRLARSFPLRSLRAAGGCRPNAP QTAASAGRVP*RRCRFLHAALDALPGSRHLPGR ASHGSQPPGLPHRPGALQRLPRP*RLQLRSDTCC DGRDLSRGGRQLAGAQDGSCSPGLHPPSSAGHK LVNREPGSWAACTCGVPAPPGGGWAKEGASEPS MDACLCLPAPYAPHTPQHWPLRGSGDTGRGSRL* RQ*GPPPHLLQSSLGAQGPSQPCHNPEPAAGAS PLWRTLL*GCPGAAGAWSV*GGGESSALREVPD HEALHPDLRGRCEDC*PVVPGRTANLR*PPRAAP ETNPTAESGAPAPPGPEHPSPAVRCRCPAAGGG GSCGAGPAWGHRHADRRLPQGEVAGP*RGLPHH PPQGGSGGGAAA*SDVPPAGPGPHPVPPAPAQLL *VPYPPGPTEPHGRF*EKFLHFPPTTTSRGCCG GIHEALPILEGRESGLGSCTRQPVPFRPARLDW LQAFPAGAAPLQPEGEGPVAEQPWAV*PGAEDIF PSGFRGRHLQTPGP*VPSSRAEKRLSLPVSPTS TQEIGLPPTWPLNVSRQICCPLTPTFTPPRQGL ALPEVNCACRIS*APAGVLQGVMRWEPSVPASSL CDPGSALSLPMAHVPALDGM*VGPTSWSCGGAC SHEWQVVPDVQCLISCTAVNCGSCRAPCPECPE ENHAYLHCI*EP*AGGCGSCQQGAYCLVCCRHLE RERAWVGAGRKIIFNYGASVLQRPEPGSVLLPL RPVLSTMSQK*VISCHSRRRAL*WFQTLSPHIA PGSGISPAPLAPSCLTHRLPI*CTQAPLWPTPSP SH*GRSTEVGWTWVPSTSLISPGTLWPQVLPWP CHLPGVGPPLLQALNQKPLC*ARISQTTHHIKK YFENMQ*S*LLGCSFCTRRAHQVHPQNTSHAVLL WSCASPPPPT*RCYIPIVLLSLECHVNRTKLYL TFGLASPHSLMPLTFLQVAGYVEFVICSMPWVF Y

Frame 2

PLPSASLGLPSVSLGVSLCVRSALLEAVVPMLP KRRRARVGSPSGDAASSTPPSTRFPGVAIYLVE PRMGRSRRAFLTGLARSKGFRVLDACSSEATHV VMEETSAEEAVSWQERRMAAAPPGCTPPALLDIS WLTESLGAGQPVPVECRHRLEVAGPRKGPLSPA WMPAYACQRPTPLTHHNTGLSEALEILAEAAGF EGSEGRLLTFCRAASVLKALPSPVTTLSQLQGLP HFGEHSSRVVQELLEHGVCEEVERVRRSERYQT MKLFTQIFGVGVKTADRWYREGLRTLDDLREQP QKLTQQQKAGLQHHQDLSTPVLRSDVDALQQVVE EAVGQALPGATVTLTGGFRRGKLQGHDVDFLIT HPKEGQEAGLLPRVMCRLQDQGLILYHQHQHSC CESPTRLAQQSHMDAFERSFCIFRLPQPPGAAVG GSTRPCPSWKAVRVDLVVAPVSQFPFALLGWTG SKLFQRELRRFSRKEKGLWLNSHGLFDPEQKTF FQAASEEDIFRHLGLEYLPPEQRNA*ACLCPPLP LRKLGCPQPGH*MSPGRYAAP*PPPSPLPAKAW LFRRSIVPAGSVEPLLVCCRV**GGSPQCQPHH CVTLGLLLASPWLTFLPWMGCEWGPHRGAVVGPA VMNGKWSLMCSVSLVALQLTVAPAGHPAQNAQK RTMHTCTAFESHELEAVVRASKEPTVWCAVGIW RGRGPG*ELGGR*FSTMGLQYCSAPSQALCFCL* GLFSAQCLKNRSYPATPVAEPFNGSKP*VHT*P LALASLQPHWPRAA*LTGFLFDAPRPPCGQLPP LLTEAEALRWAGHGCPPRPSYPQAHSGLRFCPGH VIYLEWALPFFRP*IKSHFVRRGFPRPLITLKN ILKTCSKVDSWGAVFAHVGHTRSIPRTLPMLSY CGHVLLHPHPLSGATSL*FCFL*NVM*IEPNYI* PLDWPLPTA*CL*RSCRLLGMLSL*FVPCHGCS T

Sequence POLM.cAug05

ACCCGCTTCCGTCAGCCTCACTGGGGCTTCCTTCCGTCTCGCTCGGAGTTTCCCTCTGCGTTCGCTCCGCGCTGCTGGAGGCTGTCGTCCCAATGCTCCC CAAACGGCGGCGAGCGCGGGTCGGGTCCCCTAGCGGCGATGCCGCTTCCTCCACGCCGCCCTCGACGCGCTTCCCGGGAGTCGCCATCTACCTGGTCGAG CCTCGCATGGGTCGCAGCCGCCGGGCCTTCCTCACAGGCCTGGCGCGCTCCAAAGGCTTCCGCGTCCTTGACGCCTGCAGCTCCGAAGCGACACATGTTG TGATGGAAGAGACCTCAGCAGAGGAGGCCGTCAGCTGGCAGGAGCGCAGGATGGCAGCTGCTCCCCCGGGTTGCACCCCCCCAGCTCTGCTGGACATAAG CTGGTTAACAGAGAGCCTGGGAGCTGGGCAGCCTGTACCTGTGGAGTGCCGGCACCGCCTGGAGGTGGCTGGGCCAAGGAAGGGGCCTCTGAGCCCAGCA TGGATGCCTGCCTATGCCTGCCAGCGCCCTACGCCCCTCACACACCACAACACTGGCCTCTCCGAGGCTCTGGAGATACTGGCCGAGGCAGCAGGCTTTG AAGGCAGTGAGGGCCGCCTCCTCACCTTCTGCAGAGCAGCCTCGGTGCTCAAGGCCCTTCCCAGCCCTGTCACAACCCTGAGCCAGCTGCAGGGGCTTCC CCACTTTGGAGAACACTCCTCTAGGGTTGTCCAGGAGCTGCTGGAGCATGGAGTGTGTGAGGAGGTGGAGAGAGTTCGGCGCTCAGAGAGGTACCAGACC ATGAAGCTCTTCACCCAGATCTTCGGGGTCGGTGTGAAGACTGCTGACCGGTGGTACCGGGAAGGACTGCGAACCTTAGATGACCTCCGAGAGCAGCCCC AGAAACTAACCCAACAGCAGAAAGCGGCACCACCAGGACCTGAGCACCCCAGTCCTGCGGTCCGATGTAGATGCCCTGCAGCAGGTGGTGGAGGAAGCTG TGGGGCAGGCCCTGCCTGGGGCCACCGTCACGCTGACCGGCGGCTTCCGCAGGGCCTCATCCTGTACCACCAGCACCAGCACAGCTGCTGTGAGTCCCCT ACCCGCCTGGCCCAACAGAGCCACATGGACGCTTTTGAGAGAAGTTTCTGCATTTTCCGCCTACCACAACCTCCAGGGGCTGCTGTGGGGGGATCCACGA GGCCCTGCCCATCCTGGAAGGCCGTGAGAGTGGACTTGGTAGTTGCACCCGTCAGCCAGTTCCCTTTCGCCCTGCTCGGTTGGACTGGCTCCAAGCTTTT CCAGCGGGAGCTGCGCCGCTTCAGCCGGAAGGAGAAGGGCCTGTGGCTGAACAGCCATGGGCTGTTTGACCCGGAGCAGAAGACATTTTTCCAAGCGGCT TCAGAGGAAGACATCTTCAGACACCTGGGCCTTGAGTACCTTCCTCCAGAGCAGAGAAACGCCTGAGCCTGCCTGTGTCCCCCACTTCCACTCAGGAAAT TGGGCTGCCCCCAACCTGGCCACTGAATGTCTCCAGGCAGATATGCTGCCCCCTGACCCCCACCTTCACCCCTCCCCGCCAAGGCCTGGCTCTTCCGGAG GTCAATTGTGCCTGCAGGATCAGTTGAGCCCCTGCTGGTGTGCTGCAGGGTGTGATGAGGTGGGAGCCCTCAGTGCCAGCCTCATCACTGTGTGACCCTG GGTCTGCTCTTAGCCTCCCCATGGCTCACGTTCCTGCCCTGGATGGGATGTGAGTGGGGCCCACATCGTGGAGCTGTGGTGGGGCCTGCAGTCATGAATG GCAAGTGGTCCCTGATGTGCAGTGTCTCATTAGTTGCACTGCAGTTAACTGTGGCTCCTGCAGGGCACCCTGCCCAGAATGCCCAGAAGAGAACCATGCA TACCTGCACTGCATTTGAGAGCCATGAGCTGGAGGCTGTGGTTCGTGCCAGCAAGGAGCCTACTGTCTGGTGTGCTGTAGGCATCTGGAGAGGGAGAGGG CCTGGGTAGGAGCTGGGAGGAAGATAATTTTCAACTATGGGGCTTCAGTACTGCAGCGCCCCGAGCCAGGCTCTGTGCTTCTGCCTTTAAGGCCTGTTCT CAGCACAATGTCTCAAAAATAGGTCATATCCTGCCACTCCCGTCGCAGAGCCCTTTAATGGTTCCAAACCCTAAGTCCACACATAGCCCCTGGCTCTGGC ATCTCTCCAGCCCCACTGGCCCCGAGCTGCTTGACTCACCGGCTTCCTATTTGATGCACCCAGGCCCCCTTGTGGCCAACTCCCTCCCCTTCTCACTGAG GCAGAAGCACTGAGGTGGGCTGGACATGGGTGCCCTCCACGTCCCTCATATCCCCAGGCACACTCTGGCCTCAGGTTTTGCCCTGGCCATGTCATCTACC TGGAGTGGGCCCTCCCCTTCTTCAGGCCTTGAATCAAAAGCCACTTTGTTAGGCGAGGATTTCCCAGACCACTCATCACATTAAAAAATATTTTGAAAAC ATGCAGTAAAGTTGACTCTTGGGGTGCAGTTTTTGCACACGTAGGGCACACCAGGTCCATCCCCAGAACACTTCCCATGCTGTCCTATTGTGGTCATGTG CTTCTCCACCCCCACCCACTTAGCGGTGCTACATCCCTATAGTTTTGCTTTCTCTAGAATGTCATGTAAATAGAACCAAACTATATTTAACCTTTGGACT GGCCTCTCCCCACAGCCTAATGCCTTTGACGTTCCTGCAGGTTGCTGGGTATGTTGAGTTTGTAATTTGTTCCATGCCATGGGTGTTCTACAG

POLM.u.f.1 (forward primer)
POLM.u.r.6 (reverse primer)
Multiple exon skipping

Frame 0

TRFRQPHWGFLPSRSEFPSAFAPRCWRLSSQCS PNGGERGSGPLAAMPLPPRRPRRASRESPSTWSS LAWVAAAGPSSQAWRAPKASASLTPAAPKRHML *WKRPQQRRPSAGRSAGWQLLPRVAPPQLCWT* AG*QRAWELGSLYLWSAGTAWRWLGQGRGL*AQH GCLPMPASALRPSHTTTLASPRLWRYWPRQQAL KAVRAASSPSAEQPRCSRPFPALSQP*ASCRGF PTLENTPLGLSRSCWSMECVRRWREFGAQRGTRP *SSSPRSSGSV*RLLTGGTGKDCEP*MTSESSP RN*PNSRKRHHQDLSTPVLRSDVDALQQVVEEA VGQALPGATVTLTGGFRRASSCTTSTSTAAVSPL PAWPNRATWTLLREVSAFSAYHNLQGLLWGDPR GPAHPGRP*EWTW*LHPSASSLSPCSVGLAPSF SSGSCAASAGRRRACG*TAMGCLTRSRRHFSKRL QRKTSSDTWALSTFLQSRETPEPACVPHFHSGN WAAPNLATECLQADMLPPDPHLHPSPPRPGSSG GQLCLQDQLSPCWCAAGCDEVGALSASLITV*PW VCS*PPHGSRSCPGWDVSGAHIVELWWGLQS*M ASGP*CAVSH*LHCS*LWLLQGTLPRMPRREPC IPALHLRAMSWRLWFVPARSLLSGVL*ASGEGEG LGRSWEEDNFQLWGFSTAAPRARLCASAFKACS QHNVSKIGHILPLPSQSPLMVPNPKSTHSPWLW HLSSPTGPELLDSPASYLMHPGPLVANSLPFSLR QKH*GGLDMGALHVPHIPRHTLASGFALAMSST WSGPSPSSGLESKATLLGEDFPDHSSH*KIF*K HAVKLTLGVQFLHT*GTPGPSPEHFPCCPIVVMC FSTPTHLAVLHPYSFAFSRMSCK*NQTIFNLWT GLSPQPNAFDVPAGCWVC*VCNLFHAMGVLQ

Frame 1

PASVSLTGASFRLARSFPLRSLRAAGGCRPNAP QTAASAGRVP*RRCRFLHAALDALPGSRHLPGR ASHGSQPPGLPHRPGALQRLPRP*RLQLRSDTCC DGRDLSRGGRQLAGAQDGSCSPGLHPPSSAGHK LVNREPGSWAACTCGVPAPPGGGWAKEGASEPS MDACLCLPAPYAPHTPQHWPLRGSGDTGRGSRL* RQ*GPPPHLLQSSLGAQGPSQPCHNPEPAAGAS PLWRTLL*GCPGAAGAWSV*GGGESSALREVPD HEALHPDLRGRCEDC*PVVPGRTANLR*PPRAAP ETNPTAESGTTRT*APQSCGPM*MPCSRWWRKL WGRPCLGPPSR*PAASAGPHPVPPAPAQLL*VP YPPGPTEPHGRF*EKFLHFPPTTTSRGCCGGIHE ALPILEGRESGLGSCTRQPVPFRPARLDWLQAF PAGAAPLQPEGEGPVAEQPWAV*PGAEDIFPSG FRGRHLQTPGP*VPSSRAEKRLSLPVSPTSTQEI GLPPTWPLNVSRQICCPLTPTFTPPRQGLALPE VNCACRIS*APAGVLQGVMRWEPSVPASSLCDP GSALSLPMAHVPALDGM*VGPTSWSCGGACSHEW QVVPDVQCLISCTAVNCGSCRAPCPECPEENHA YLHCI*EP*AGGCGSCQQGAYCLVCCRHLERER AWVGAGRKIIFNYGASVLQRPEPGSVLLPLRPVL STMSQK*VISCHSRRRAL*WFQTLSPHIAPGSG ISPAPLAPSCLTHRLPI*CTQAPLWPTPSPSH* GRSTEVGWTWVPSTSLISPGTLWPQVLPWPCHLP GVGPPLLQALNQKPLC*ARISQTTHHIKKYFEN MQ*S*LLGCSFCTRRAHQVHPQNTSHAVLLWSC ASPPPPT*RCYIPIVLLSLECHVNRTKLYLTFGL ASPHSLMPLTFLQVAGYVEFVICSMPWVFY

Frame 2

PLPSASLGLPSVSLGVSLCVRSALLEAVVPMLP KRRRARVGSPSGDAASSTPPSTRFPGVAIYLVE PRMGRSRRAFLTGLARSKGFRVLDACSSEATHV VMEETSAEEAVSWQERRMAAAPPGCTPPALLDIS WLTESLGAGQPVPVECRHRLEVAGPRKGPLSPA WMPAYACQRPTPLTHHNTGLSEALEILAEAAGF EGSEGRLLTFCRAASVLKALPSPVTTLSQLQGLP HFGEHSSRVVQELLEHGVCEEVERVRRSERYQT MKLFTQIFGVGVKTADRWYREGLRTLDDLREQP QKLTQQQKAAPPGPEHPSPAVRCRCPAAGGGGSC GAGPAWGHRHADRRLPQGLILYHQHQHSCCESP TRLAQQSHMDAFERSFCIFRLPQPPGAAVGGST RPCPSWKAVRVDLVVAPVSQFPFALLGWTGSKLF QRELRRFSRKEKGLWLNSHGLFDPEQKTFFQAA SEEDIFRHLGLEYLPPEQRNA*ACLCPPLPLRK LGCPQPGH*MSPGRYAAP*PPPSPLPAKAWLFRR SIVPAGSVEPLLVCCRV**GGSPQCQPHHCVTL GLLLASPWLTFLPWMGCEWGPHRGAVVGPAVMN GKWSLMCSVSLVALQLTVAPAGHPAQNAQKRTMH TCTAFESHELEAVVRASKEPTVWCAVGIWRGRG PG*ELGGR*FSTMGLQYCSAPSQALCFCL*GLF SAQCLKNRSYPATPVAEPFNGSKP*VHT*PLALA SLQPHWPRAA*LTGFLFDAPRPPCGQLPPLLTE AEALRWAGHGCPPRPSYPQAHSGLRFCPGHVIY LEWALPFFRP*IKSHFVRRGFPRPLITLKNILKT CSKVDSWGAVFAHVGHTRSIPRTLPMLSYCGHV LLHPHPLSGATSL*FCFL*NVM*IEPNYI*PLD WPLPTA*CL*RSCRLLGMLSL*FVPCHGCST

Sequence POLM.dAug05

ACCCGCTTCCGTCAGCCTCACTGGGGCTTCCTTCCGTCTCGCTCGGAGTTTCCCTCTGCGTTCGCTCCGCGCTGCTGGAGGCTGTCGTCCCAATGCTCCC CAAACGGCGGCGAGCGCGGGTCGGGTCCCCTAGCGGCGATGCCGCTTCCTCCACGCCGCCCTCGACGCGCTTCCCGGGAGTCGCCATCTACCTGGTCGAG CCTCGCATGGGTCGCAGCCGCCGGGCCTTCCTCACAGGCCTGGCGCGCTCCAAAGGCTTCCGCGTCCTTGACGCCTGCAGCTCCGAAGCGACACATGTTG TGATGGAAGAGACCTCAGCAGAGGAGGCCGTCAGCTGGCAGGAGCGCAGGATGGCAGCTGCTCCCCCGGGTTGCACCCCCCCAGCTCTGCTGGACATAAG CTGGTTAACAGAGAGCCTGGGAGCTGGGCAGCCTGTACCTGTGGAGTGCCGGCACCGCCTGGAGGTGGCTGGGCCAAGGAAGGGGCCTCTGAGCCCAGCA TGGATGCCTGCCTATGCCTGCCAGCGCCCTACGCCCCTCACACACCACAACACTGGCCTCTCCGAGGCTCTGGAGATACTGGCCGAGGCAGCAGGCTTTG AAGGCAGTGAGGGCCGCCTCCTCACCTTCTGCAGAGCAGCCTCGGTGCTCAAGGCCCTTCCCAGCCCTGTCACAACCCTGAGCCAGCTGCAGGGGCTTCC CCACTTTGGAGAACACTCCTCTAGGGTTGTCCAGGAGCTGCTGGAGCATGGAGTGTGTGAGGAGGTGGAGAGAGTTCGGCGCTCAGAGAGGTACCAGACC ATGAAGGGCCTCATCCTGTACCACCAGCACCAGCACAGCTGCTGTGAGTCCCCTACCCGCCTGGCCCAACAGAGCCACATGGACGCTTTTGAGAGAAGTT TCTGCATTTTCCGCCTACCACAACCTCCAGGGGCTGCTGTGGGGGGATCCACGAGGCCCTGCCCATCCTGGAAGGCCGTGAGAGTGGACTTGGTAGTTGC ACCCGTCAGCCAGTTCCCTTTCGCCCTGCTCGGTTGGACTGGCTCCAAGCTTTTCCAGCGGGAGCTGCGCCGCTTCAGCCGGAAGGAGAAGGGCCTGTGG CTGAACAGCCATGGGCTGTTTGACCCGGAGCAGGGAAGCAGCAGTGGCAAGACTCCTAGGTCACGGAAGTCCTGCTTCTGTTGCAGAAGACATTTTTCCA AGCGGCTTCAGAGGAAGACATCTTCAGACACCTGGGCCTTGAGTACCTTCCTCCAGAGCAGAGAAACGCCTGAGCCTGCCTGTGTCCCCCACTTCCACTC AGGAAATTGGGCTGCCCCCAACCTGGCCACTGAATGTCTCCAGGCAGATATGCTGCCCCCTGACCCCCACCTTCACCCCTCCCCGCCAAGGCCTGGCTCT TCCGGAGGTCAATTGTGCCTGCAGGATCAGTTGAGCCCCTGCTGGTGTGCTGCAGGGTGTGATGAGGTGGGAGCCCTCAGTGCCAGCCTCATCACTGTGT GACCCTGGGTCTGCTCTTAGCCTCCCCATGGCTCACGTTCCTGCCCTGGATGGGATGTGAGTGGGGCCCACATCGTGGAGCTGTGGTGGGGCCTGCAGTC ATGAATGGCAAGTGGTCCCTGATGTGCAGTGTCTCATTAGTTGCACTGCAGTTAACTGTGGCTCCTGCAGGGCACCCTGCCCAGAATGCCCAGAAGAGAA CCATGCATACCTGCACTGCATTTGAGAGCCATGAGCTGGAGGCTGTGGTTCGTGCCAGCAAGGAGCCTACTGTCTGGTGTGCTGTAGGCATCTGGAGAGG GAGAGGGCCTGGGTAGGAGCTGGGAGGAAGATAATTTTCAACTATGGGGCTTCAGTACTGCAGCGCCCCGAGCCAGGCTCTGTGCTTCTGCCTTTAAGGC CTGTTCTCAGCACAATGTCTCAAAAATAGGTCATATCCTGCCACTCCCGTCGCAGAGCCCTTTAATGGTTCCAAACCCTAAGTCCACACATAGCCCCTGG CTCTGGCATCTCTCCAGCCCCACTGGCCCCGAGCTGCTTGACTCACCGGCTTCCTATTTGATGCACCCAGGCCCCCTTGTGGCCAACTCCCTCCCCTTCT CACTGAGGCAGAAGCACTGAGGTGGGCTGGACATGGGTGCCCTCCACGTCCCTCATATCCCCAGGCACACTCTGGCCTCAGGTTTTGCCCTGGCCATGTC ATCTACCTGGAGTGGGCCCTCCCCTTCTTCAGGCCTTGAATCAAAAGCCACTTTGTTAGGCGAGGATTTCCCAGACCACTCATCACATTAAAAAATATTT TGAAAACATGCAGTAAAGTTGACTCTTGGGGTGCAGTTTTTGCACACGTAGGGCACACCAGGTCCATCCCCAGAACACTTCCCATGCTGTCCTATTGTGG TCATGTGCTTCTCCACCCCCACCCACTTAGCGGTGCTACATCCCTATAGTTTTGCTTTCTCTAGAATGTCATGTAAATAGAACCAAACTATATTTAACCT TTGGACTGGCCTCTCCCCACAGCCTAATGCCTTTGACGTTCCTGCAGGTTGCTGGGTATGTTGAGTTTGTAATTTGTTCCATGCCATGGGTGTTCTACAG

POLM.u.f.1 (forward primer)
POLM.u.r.6 (reverse primer)
Multiple exon skipping

Frame 0

TRFRQPHWGFLPSRSEFPSAFAPRCWRLSSQCS PNGGERGSGPLAAMPLPPRRPRRASRESPSTWSS LAWVAAAGPSSQAWRAPKASASLTPAAPKRHML *WKRPQQRRPSAGRSAGWQLLPRVAPPQLCWT* AG*QRAWELGSLYLWSAGTAWRWLGQGRGL*AQH GCLPMPASALRPSHTTTLASPRLWRYWPRQQAL KAVRAASSPSAEQPRCSRPFPALSQP*ASCRGF PTLENTPLGLSRSCWSMECVRRWREFGAQRGTRP *RASSCTTSTSTAAVSPLPAWPNRATWTLLREV SAFSAYHNLQGLLWGDPRGPAHPGRP*EWTW*L HPSASSLSPCSVGLAPSFSSGSCAASAGRRRACG *TAMGCLTRSREAAVARLLGHGSPASVAEDIFP SGFRGRHLQTPGP*VPSSRAEKRLSLPVSPTST QEIGLPPTWPLNVSRQICCPLTPTFTPPRQGLAL PEVNCACRIS*APAGVLQGVMRWEPSVPASSLC DPGSALSLPMAHVPALDGM*VGPTSWSCGGACS HEWQVVPDVQCLISCTAVNCGSCRAPCPECPEEN HAYLHCI*EP*AGGCGSCQQGAYCLVCCRHLER ERAWVGAGRKIIFNYGASVLQRPEPGSVLLPLR PVLSTMSQK*VISCHSRRRAL*WFQTLSPHIAPG SGISPAPLAPSCLTHRLPI*CTQAPLWPTPSPS H*GRSTEVGWTWVPSTSLISPGTLWPQVLPWPC HLPGVGPPLLQALNQKPLC*ARISQTTHHIKKYF ENMQ*S*LLGCSFCTRRAHQVHPQNTSHAVLLW SCASPPPPT*RCYIPIVLLSLECHVNRTKLYLT FGLASPHSLMPLTFLQVAGYVEFVICSMPWVFY

Frame 1

PASVSLTGASFRLARSFPLRSLRAAGGCRPNAP QTAASAGRVP*RRCRFLHAALDALPGSRHLPGR ASHGSQPPGLPHRPGALQRLPRP*RLQLRSDTCC DGRDLSRGGRQLAGAQDGSCSPGLHPPSSAGHK LVNREPGSWAACTCGVPAPPGGGWAKEGASEPS MDACLCLPAPYAPHTPQHWPLRGSGDTGRGSRL* RQ*GPPPHLLQSSLGAQGPSQPCHNPEPAAGAS PLWRTLL*GCPGAAGAWSV*GGGESSALREVPD HEGPHPVPPAPAQLL*VPYPPGPTEPHGRF*EKF LHFPPTTTSRGCCGGIHEALPILEGRESGLGSC TRQPVPFRPARLDWLQAFPAGAAPLQPEGEGPV AEQPWAV*PGAGKQQWQDS*VTEVLLLLQKTFFQ AASEEDIFRHLGLEYLPPEQRNA*ACLCPPLPL RKLGCPQPGH*MSPGRYAAP*PPPSPLPAKAWL FRRSIVPAGSVEPLLVCCRV**GGSPQCQPHHCV TLGLLLASPWLTFLPWMGCEWGPHRGAVVGPAV MNGKWSLMCSVSLVALQLTVAPAGHPAQNAQKR TMHTCTAFESHELEAVVRASKEPTVWCAVGIWRG RGPG*ELGGR*FSTMGLQYCSAPSQALCFCL*G LFSAQCLKNRSYPATPVAEPFNGSKP*VHT*PL ALASLQPHWPRAA*LTGFLFDAPRPPCGQLPPLL TEAEALRWAGHGCPPRPSYPQAHSGLRFCPGHV IYLEWALPFFRP*IKSHFVRRGFPRPLITLKNI LKTCSKVDSWGAVFAHVGHTRSIPRTLPMLSYCG HVLLHPHPLSGATSL*FCFL*NVM*IEPNYI*P LDWPLPTA*CL*RSCRLLGMLSL*FVPCHGCST

Frame 2

PLPSASLGLPSVSLGVSLCVRSALLEAVVPMLP KRRRARVGSPSGDAASSTPPSTRFPGVAIYLVE PRMGRSRRAFLTGLARSKGFRVLDACSSEATHV VMEETSAEEAVSWQERRMAAAPPGCTPPALLDIS WLTESLGAGQPVPVECRHRLEVAGPRKGPLSPA WMPAYACQRPTPLTHHNTGLSEALEILAEAAGF EGSEGRLLTFCRAASVLKALPSPVTTLSQLQGLP HFGEHSSRVVQELLEHGVCEEVERVRRSERYQT MKGLILYHQHQHSCCESPTRLAQQSHMDAFERS FCIFRLPQPPGAAVGGSTRPCPSWKAVRVDLVVA PVSQFPFALLGWTGSKLFQRELRRFSRKEKGLW LNSHGLFDPEQGSSSGKTPRSRKSCFCCRRHFS KRLQRKTSSDTWALSTFLQSRETPEPACVPHFHS GNWAAPNLATECLQADMLPPDPHLHPSPPRPGS SGGQLCLQDQLSPCWCAAGCDEVGALSASLITV *PWVCS*PPHGSRSCPGWDVSGAHIVELWWGLQS *MASGP*CAVSH*LHCS*LWLLQGTLPRMPRRE PCIPALHLRAMSWRLWFVPARSLLSGVL*ASGE GEGLGRSWEEDNFQLWGFSTAAPRARLCASAFKA CSQHNVSKIGHILPLPSQSPLMVPNPKSTHSPW LWHLSSPTGPELLDSPASYLMHPGPLVANSLPF SLRQKH*GGLDMGALHVPHIPRHTLASGFALAMS STWSGPSPSSGLESKATLLGEDFPDHSSH*KIF *KHAVKLTLGVQFLHT*GTPGPSPEHFPCCPIV VMCFSTPTHLAVLHPYSFAFSRMSCK*NQTIFNL WTGLSPQPNAFDVPAGCWVC*VCNLFHAMGVLQ

Sequence POLM.eAug05

ACCCGCTTCCGTCAGCCTCACTGGGGCTTCCTTCCGTCTCGCTCGGAGTTTCCCTCTGCGTTCGCTCCGCGCTGCTGGAGGCTGTCGTCCCAATGCTCCC CAAACGGCGGCGAGCGCGGGTCGGGTCCCCTAGCGGCGATGCCGCTTCCTCCACGCCGCCCTCGACGCGCTTCCCGGGAGTCGCCATCTACCTGGTCGAG CCTCGCATGGGTCGCAGCCGCCGGGCCTTCCTCACAGGCCTGGCGCGCTCCAAAGGCTTCCGCGTCCTTGACGCCTGCAGCTCCGAAGCGACACATGTTG TGATGGAAGAGACCTCAGCAGAGGAGGCCGTCAGCTGGCAGGAGCGCAGGATGGCAGCTGCTCCCCCGGGTTGCACCCCCCCAGCTCTGCTGGACATAAG CTGGTTAACAGAGAGCCTGGGAGCTGGGCAGCCTGTACCTGTGGAGTGCCGGCACCGCCTGGAGGTGGCTGGGCCAAGGAAGGGGCCTCTGAGCCCAGCA TGGATGCCTGCCTATGCCTGCCAGCGCCCTACGCCCCTCACACACCACAACACTGGCCTCTCCGAGGCTCTGGAGATACTGGCCGAGGCAGCAGGCTTTG AAGGCAGTGAGGGCCGCCTCCTCACCTTCTGCAGAGCAGCCTCGGTGCTCAAGGCCCTTCCCAGCCCTGTCACAACCCTGAGCCAGCTGCAGGGGCTTCC CCACTTTGGAGAACACTCCTCTAGGGTTGTCCAGGAGCTGCTGGAGCATGGAGTGTGTGAGGAGGTGGAGAGAGTTCGGCGCTCAGAGAGGTACCAGACC ATGAAGGTGCGCAGGGCAGGGCTCGGACCTACCCCAGGAATGTCCTGCCCTGGGAATGACAACACAGTCCACACCATGCACGGGGAGGCAAACAGGGGCA GCTGACCCAGCCCAGGGGTCAGAGAAGGTCTTGCCGAGGAAGTGGCAGCTAAGCTGATACCTGATATGCACAAGGCAGCCAAGTGGAGACAGGCTGGAGT ACAGTGGTGCGATCACAGCTCACGGCAGCCTTGACTTCCTGGGCTCAAACCATCCTCCCACCTCAGTCTCTGGAGTAGCTGGGACTACAGGCATGTGCCA CCACACCAGCTCTTCACCCAGATCTTCGGGGTCGGTGTGAAGACTGCTGACCGGTGGTACCGGGAAGGACTGCGAACCTTAGATGACCTCCGAGAGCAGC CCCAGAAACTAACCCAACAGCAGAAAGCGGGGCTCCAGCACCACCAGGACCTGAGCACCCCAGTCCTGCGGTCCGATGTAGATGCCCTGCAGCAGGTGGT GGAGGAAGCTGTGGGGCAGGCCCTGCCTGGGGCCACCGTCACGCTGACCGGCGGCTTCCGCAGGTAAGAAGGCCCGACGTGCCCCTCTGGCTTGGCTTCA GACCCCTCCCCAGTGAAGGGACCTGAGTCGGGGACCCTGAAGCGGGCCCACTCGTTGGAAGGGATGTGGCCTGGGGCTGGAGTGAGCAGGCCGTGTCCCT GAGTCAGAGTCAGACTGACGCCCTCAGGGGGAAGTTGCAGGGCCATGACGTGGACTTCCTCATCACCCACCCCAAGGAGGGTCAGGAGGCGGGGCTGCTG CCTAGAGTGATGTGCCGCCTGCAGGACCAGGTGAGGGCCTCCCTGCTCCCCAAGAGCCACCGCTGGCCAATGCCACTGCCTCCTGCTCTCTCCACACCAC CGGATGGTCTCAGCCACGCCCGCCTCTCCCCACAGGGCCTCATCCTGTACCACCAGCACCAGCACAGCTGCTGTGAGTCCCCTACCCGCCTGGCCCAACA GAGCCACATGGACGCTTTTGAGAGAAGTTTCTGCATTTTCCGCCTACCACAACCTCCAGGGGCTGCTGTGGGGGGATCCACGAGGCCCTGCCCATCCTGG AAGGCCGTGAGAGTGGACTTGGTAGTTGCACCCGTCAGCCAGTTCCCTTTCGCCCTGCTCGGTTGGACTGGCTCCAAGCTTTTCCAGCGGGAGCTGCGCC GCTTCAGCCGGAAGGAGAAGGGCCTGTGGCTGAACAGCCATGGGCTGTTTGACCCGGAGCAGGGAAGCAGCAGTGGCAAGACTCCTAGGTCACGGAAGTC CTGCTTCTGTTGCAGAAGACATTTTTCCAAGCGGCTTCAGAGGAAGACATCTTCAGACACCTGGGCCTTGAGTACCTTCCTCCAGAGCAGAGAAACGCCT GAGCCTGCCTGTGTCCCCCACTTCCACTCAGGAAATTGGGCTGCCCCCAACCTGGCCACTGAATGTCTCCAGGCAGATATGCTGCCCCCTGACCCCCACC TTCACCCCTCCCCGCCAAGGCCTGGCTCTTCCGGAGGTCAATTGTGCCTGCAGGATCAGTTGAGCCCCTGCTGGTGTGCTGCAGGGTGTGATGAGGTGGG AGCCCTCAGTGCCAGCCTCATCACTGTGTGACCCTGGGTCTGCTCTTAGCCTCCCCATGGCTCACGTTCCTGCCCTGGATGGGATGTGAGTGGGGCCCAC ATCGTGGAGCTGTGGTGGGGCCTGCAGTCATGAATGGCAAGTGGTCCCTGATGTGCAGTGTCTCATTAGTTGCACTGCAGTTAACTGTGGCTCCTGCAGG GCACCCTGCCCAGAATGCCCAGAAGAGAACCATGCATACCTGCACTGCATTTGAGAGCCATGAGCTGGAGGCTGTGGTTCGTGCCAGCAAGGAGCCTACT GTCTGGTGTGCTGTAGGCATCTGGAGAGGGAGAGGGCCTGGGTAGGAGCTGGGAGGAAGATAATTTTCAACTATGGGGCTTCAGTACTGCAGCGCCCCGA GCCAGGCTCTGTGCTTCTGCCTTTAAGGCCTGTTCTCAGCACAATGTCTCAAAAATAGGTCATATCCTGCCACTCCCGTCGCAGAGCCCTTTAATGGTTC CAAACCCTAAGTCCACACATAGCCCCTGGCTCTGGCATCTCTCCAGCCCCACTGGCCCCGAGCTGCTTGACTCACCGGCTTCCTATTTGATGCACCCAGG CCCCCTTGTGGCCAACTCCCTCCCCTTCTCACTGAGGCAGAAGCACTGAGGTGGGCTGGACATGGGTGCCCTCCACGTCCCTCATATCCCCAGGCACACT CTGGCCTCAGGTTTTGCCCTGGCCATGTCATCTACCTGGAGTGGGCCCTCCCCTTCTTCAGGCCTTGAATCAAAAGCCACTTTGTTAGGCGAGGATTTCC CAGACCACTCATCACATTAAAAAATATTTTGAAAACATGCAGTAAAGTTGACTCTTGGGGTGCAGTTTTTGCACACGTAGGGCACACCAGGTCCATCCCC AGAACACTTCCCATGCTGTCCTATTGTGGTCATGTGCTTCTCCACCCCCACCCACTTAGCGGTGCTACATCCCTATAGTTTTGCTTTCTCTAGAATGTCA TGTAAATAGAACCAAACTATATTTAACCTTTGGACTGGCCTCTCCCCACAGCCTAATGCCTTTGACGTTCCTGCAGGTTGCTGGGTATGTTGAGTTTGTA ATTTGTTCCATGCCATGGGTGTTCTACAG

POLM.u.f.1 (forward primer)
POLM.u.r.6 (reverse primer)
Multiple exon skipping

Frame 0

TRFRQPHWGFLPSRSEFPSAFAPRCWRLSSQCS PNGGERGSGPLAAMPLPPRRPRRASRESPSTWSS LAWVAAAGPSSQAWRAPKASASLTPAAPKRHML *WKRPQQRRPSAGRSAGWQLLPRVAPPQLCWT* AG*QRAWELGSLYLWSAGTAWRWLGQGRGL*AQH GCLPMPASALRPSHTTTLASPRLWRYWPRQQAL KAVRAASSPSAEQPRCSRPFPALSQP*ASCRGF PTLENTPLGLSRSCWSMECVRRWREFGAQRGTRP *RCAGQGSDLPQECPALGMTTQSTPCTGRQTGA ADPAQGSEKVLPRKWQLS*YLICTRQPSGDRLE YSGAITAHGSLDFLGSNHPPTSVSGVAGTTGMCH HTSSSPRSSGSV*RLLTGGTGKDCEP*MTSESS PRN*PNSRKRGSSTTRT*APQSCGPM*MPCSRW WRKLWGRPCLGPPSR*PAASAGKKARRAPLAWLQ TPPQ*RDLSRGP*SGPTRWKGCGLGLE*AGRVP ESESD*RPQGEVAGP*RGLPHHPPQGGSGGGAA A*SDVPPAGPGEGLPAPQEPPLANATASCSLHTT GWSQPRPPLPTGPHPVPPAPAQLL*VPYPPGPT EPHGRF*EKFLHFPPTTTSRGCCGGIHEALPIL EGRESGLGSCTRQPVPFRPARLDWLQAFPAGAAP LQPEGEGPVAEQPWAV*PGAGKQQWQDS*VTEV LLLLQKTFFQAASEEDIFRHLGLEYLPPEQRNA *ACLCPPLPLRKLGCPQPGH*MSPGRYAAP*PPP SPLPAKAWLFRRSIVPAGSVEPLLVCCRV**GG SPQCQPHHCVTLGLLLASPWLTFLPWMGCEWGP HRGAVVGPAVMNGKWSLMCSVSLVALQLTVAPAG HPAQNAQKRTMHTCTAFESHELEAVVRASKEPT VWCAVGIWRGRGPG*ELGGR*FSTMGLQYCSAP SQALCFCL*GLFSAQCLKNRSYPATPVAEPFNGS KP*VHT*PLALASLQPHWPRAA*LTGFLFDAPR PPCGQLPPLLTEAEALRWAGHGCPPRPSYPQAH SGLRFCPGHVIYLEWALPFFRP*IKSHFVRRGFP RPLITLKNILKTCSKVDSWGAVFAHVGHTRSIP RTLPMLSYCGHVLLHPHPLSGATSL*FCFL*NV M*IEPNYI*PLDWPLPTA*CL*RSCRLLGMLSL* FVPCHGCST

Frame 1

PASVSLTGASFRLARSFPLRSLRAAGGCRPNAP QTAASAGRVP*RRCRFLHAALDALPGSRHLPGR ASHGSQPPGLPHRPGALQRLPRP*RLQLRSDTCC DGRDLSRGGRQLAGAQDGSCSPGLHPPSSAGHK LVNREPGSWAACTCGVPAPPGGGWAKEGASEPS MDACLCLPAPYAPHTPQHWPLRGSGDTGRGSRL* RQ*GPPPHLLQSSLGAQGPSQPCHNPEPAAGAS PLWRTLL*GCPGAAGAWSV*GGGESSALREVPD HEGAQGRARTYPRNVLPWE*QHSPHHARGGKQGQ LTQPRGQRRSCRGSGS*ADT*YAQGSQVETGWS TVVRSQLTAALTSWAQTILPPQSLE*LGLQACA TTPALHPDLRGRCEDC*PVVPGRTANLR*PPRAA PETNPTAESGAPAPPGPEHPSPAVRCRCPAAGG GGSCGAGPAWGHRHADRRLPQVRRPDVPLWLGF RPLPSEGT*VGDPEAGPLVGRDVAWGWSEQAVSL SQSQTDALRGKLQGHDVDFLITHPKEGQEAGLL PRVMCRLQDQVRASLLPKSHRWPMPLPPALSTP PDGLSHARLSPQGLILYHQHQHSCCESPTRLAQQ SHMDAFERSFCIFRLPQPPGAAVGGSTRPCPSW KAVRVDLVVAPVSQFPFALLGWTGSKLFQRELR RFSRKEKGLWLNSHGLFDPEQGSSSGKTPRSRKS CFCCRRHFSKRLQRKTSSDTWALSTFLQSRETP EPACVPHFHSGNWAAPNLATECLQADMLPPDPH LHPSPPRPGSSGGQLCLQDQLSPCWCAAGCDEVG ALSASLITV*PWVCS*PPHGSRSCPGWDVSGAH IVELWWGLQS*MASGP*CAVSH*LHCS*LWLLQ GTLPRMPRREPCIPALHLRAMSWRLWFVPARSLL SGVL*ASGEGEGLGRSWEEDNFQLWGFSTAAPR ARLCASAFKACSQHNVSKIGHILPLPSQSPLMV PNPKSTHSPWLWHLSSPTGPELLDSPASYLMHPG PLVANSLPFSLRQKH*GGLDMGALHVPHIPRHT LASGFALAMSSTWSGPSPSSGLESKATLLGEDF PDHSSH*KIF*KHAVKLTLGVQFLHT*GTPGPSP EHFPCCPIVVMCFSTPTHLAVLHPYSFAFSRMS CK*NQTIFNLWTGLSPQPNAFDVPAGCWVC*VC NLFHAMGVLQ

Frame 2

PLPSASLGLPSVSLGVSLCVRSALLEAVVPMLP KRRRARVGSPSGDAASSTPPSTRFPGVAIYLVE PRMGRSRRAFLTGLARSKGFRVLDACSSEATHV VMEETSAEEAVSWQERRMAAAPPGCTPPALLDIS WLTESLGAGQPVPVECRHRLEVAGPRKGPLSPA WMPAYACQRPTPLTHHNTGLSEALEILAEAAGF EGSEGRLLTFCRAASVLKALPSPVTTLSQLQGLP HFGEHSSRVVQELLEHGVCEEVERVRRSERYQT MKVRRAGLGPTPGMSCPGNDNTVHTMHGEANRG S*PSPGVREGLAEEVAAKLIPDMHKAAKWRQAGV QWCDHSSRQP*LPGLKPSSHLSLWSSWDYRHVP PHQLFTQIFGVGVKTADRWYREGLRTLDDLREQ PQKLTQQQKAGLQHHQDLSTPVLRSDVDALQQVV EEAVGQALPGATVTLTGGFRR*EGPTCPSGLAS DPSPVKGPESGTLKRAHSLEGMWPGAGVSRPCP *VRVRLTPSGGSCRAMTWTSSSPTPRRVRRRGCC LE*CAACRTR*GPPCSPRATAGQCHCLLLSPHH RMVSATPASPHRASSCTTSTSTAAVSPLPAWPN RATWTLLREVSAFSAYHNLQGLLWGDPRGPAHPG RP*EWTW*LHPSASSLSPCSVGLAPSFSSGSCA ASAGRRRACG*TAMGCLTRSREAAVARLLGHGS PASVAEDIFPSGFRGRHLQTPGP*VPSSRAEKRL SLPVSPTSTQEIGLPPTWPLNVSRQICCPLTPT FTPPRQGLALPEVNCACRIS*APAGVLQGVMRW EPSVPASSLCDPGSALSLPMAHVPALDGM*VGPT SWSCGGACSHEWQVVPDVQCLISCTAVNCGSCR APCPECPEENHAYLHCI*EP*AGGCGSCQQGAY CLVCCRHLERERAWVGAGRKIIFNYGASVLQRPE PGSVLLPLRPVLSTMSQK*VISCHSRRRAL*WF QTLSPHIAPGSGISPAPLAPSCLTHRLPI*CTQ APLWPTPSPSH*GRSTEVGWTWVPSTSLISPGTL WPQVLPWPCHLPGVGPPLLQALNQKPLC*ARIS QTTHHIKKYFENMQ*S*LLGCSFCTRRAHQVHP QNTSHAVLLWSCASPPPPT*RCYIPIVLLSLECH VNRTKLYLTFGLASPHSLMPLTFLQVAGYVEFV ICSMPWVFY

Sequence POLM.hAug05

ACCCGCTTCCGTCAGCCTCACTGGGGCTTCCTTCCGTCTCGCTCGGAGTTTCCCTCTGCGTTCGCTCCGCGCTGCTGGAGGCTGTCGTCCCAATGCTCCC CAAACGGCGGCGAGCGCGGGTCGGGTCCCCTAGCGGCGATGCCGCTTCCTCCACGCCGCCCTCGACGCGCTTCCCGGGAGTCGCCATCTACCTGGTCGAG CCTCGCATGGGTCGCAGCCGCCGGGCCTTCCTCACAGGCCTGGCGCGCTCCAAAGGCTTCCGCGTCCTTGACGCCTGCAGCTCCGAAGCGACACATGTTG TGATGGAAGAGACCTCAGCAGAGGAGGCCGTCAGCTGGCAGGAGCGCAGGATGGCAGCTGCTCCCCCGGGTTGCACCCCCCCAGCTCTGCTGGACATAAG CTGGTTAACAGAGAGCCTGGGAGCTGGGCAGCCTGTACCTGTGGAGTGCCGGCACCGCCTGGAGGTGGCTGGGCCAAGGAAGGGGCCTCTGAGCCCAGCA TGGATGCCTGCCTATGCCTGCCAGCGCCCTACGCCCCTCACACACCACAACACTGGCCTCTCCGAGGCTCTGGAGATACTGGCCGAGGCAGCAGGCTTTG AAGGCAGTGAGGGCCGCCTCCTCACCTTCTGCAGAGCAGCCTCGGTGCTCAAGGCCCTTCCCAGCCCTGTCACAACCCTGAGCCAGCTGCAGGGGCTTCC CCACTTTGGAGAACACTCCTCTAGGGTTGTCCAGGAGCTGCTGGAGCATGGAGTGTGTGAGGAGGTGGAGAGAGTTCGGCGCTCAGAGAGCTCTTCACCC AGATCTTCGGGGTCGGTGTGAAGACTGCTGACCGGTGGTACCGGGAAGGACTGCGAACCTTAGATGACCTCCGAGAGCAGCCCCAGAAACTAACCCAACA GCAGAAAGCGGGGCTCCAGCACCACCAGGACCTGAGCACCCCAGTCCTGCGGTCCGATGTAGATGCCCTGCAGCAGGTGGTGGAGGAAGCTGTGGGGCAG GCCCTGCCTGGGGCCACCGTCACGCTGACCGGCGGCTTCCGCAGGGCCATGACGTGGACTTCCTCATCACCCACCCCAAGGAGGGTCAGGAGGCGGGGCT GCTGCCTAGAGTGATGTGCCGCCTGCAGGACCAGGGCCTCATCCTGTACCACCAGCACCAGCACAGCTGCTGTGAGTCCCCTACCCGCCTGGCCCAACAG AGCCACATGGACGCTTTTGAGAGAAGTTTCTGCATTTTCCGCCTACCACAACCTCCAGGGGCTGCTGTGGGGGGATCCACGAGGCCCTGCCCATCCTGGA AGGCCGTGAGAGTGGACTTGGTAGTTGCACCCGTCAGCCAGTTCCCTTTCGCCCTGCTCGGTTGGACTGGCTCCAAGCTTTTCCAGCGGGAGCTGCGCCG CTTCAGCCGGAAGGAGAAGGGCCTGTGGCTGAACAGCCATGGGCTGTTTGACCCGGAGCAGAAGACATTTTTCCAAGCGGCTTCAGAGGAAGACATCTTC AGACACCTGGGCCTTGAGTACCTTCCTCCAGAGCAGAGAAACGCCTGAGCCTGCCTGTGTCCCCCACTTCCACTCAGGAAATTGGGCTGCCCCCAACCTG GCCACTGAATGTCTCCAGGCAGATATGCTGCCCCCTGACCCCCACCTTCACCCCTCCCCGCCAAGGCCTGGCTCTTCCGGAGGTCAATTGTGCCTGCAGG ATCAGTTGAGCCCCTGCTGGTGTGCTGCAGGGTGTGATGAGGTGGGAGCCCTCAGTGCCAGCCTCATCACTGTGTGACCCTGGGTCTGCTCTTAGCCTCC CCATGGCTCACGTTCCTGCCCTGGATGGGATGTGAGTGGGGCCCACATCGTGGAGCTGTGGTGGGGCCTGCAGTCATGAATGGCAAGTGGTCCCTGATGT GCAGTGTCTCATTAGTTGCACTGCAGTTAACTGTGGCTCCTGCAGGGCACCCTGCCCAGAATGCCCAGAAGAGAACCATGCATACCTGCACTGCATTTGA GAGCCATGAGCTGGAGGCTGTGGTTCGTGCCAGCAAGGAGCCTACTGTCTGGTGTGCTGTAGGCATCTGGAGAGGGAGAGGGCCTGGGTAGGAGCTGGGA GGAAGATAATTTTCAACTATGGGGCTTCAGTACTGCAGCGCCCCGAGCCAGGCTCTGTGCTTCTGCCTTTAAGGCCTGTTCTCAGCACAATGTCTCAAAA ATAGGTCATATCCTGCCACTCCCGTCGCAGAGCCCTTTAATGGTTCCAAACCCTAAGTCCACACATAGCCCCTGGCTCTGGCATCTCTCCAGCCCCACTG GCCCCGAGCTGCTTGACTCACCGGCTTCCTATTTGATGCACCCAGGCCCCCTTGTGGCCAACTCCCTCCCCTTCTCACTGAGGCAGAAGCACTGAGGTGG GCTGGACATGGGTGCCCTCCACGTCCCTCATATCCCCAGGCACACTCTGGCCTCAGGTTTTGCCCTGGCCATGTCATCTACCTGGAGTGGGCCCTCCCCT TCTTCAGGCCTTGAATCAAAAGCCACTTTGTTAGGCGAGGATTTCCCAGACCACTCATCACATTAAAAAATATTTTGAAAACATGCAGTAAAGTTGACTC TTGGGGTGCAGTTTTTGCACACGTAGGGCACACCAGGTCCATCCCCAGAACACTTCCCATGCTGTCCTATTGTGGTCATGTGCTTCTCCACCCCCACCCA CTTAGCGGTGCTACATCCCTATAGTTTTGCTTTCTCTAGAATGTCATGTAAATAGAACCAAACTATATTTAACCTTTGGACTGGCCTCTCCCCACAGCCT AATGCCTTTGACGTTCCTGCAGGTTGCTGGGTATGTTGAGTTTGTAATTTGTTCCATGCCATGGGTGTTCTACAG

POLM.u.f.1 (forward primer)
POLM.u.r.6 (reverse primer)
Multiple exon skipping

Frame 0

TRFRQPHWGFLPSRSEFPSAFAPRCWRLSSQCS PNGGERGSGPLAAMPLPPRRPRRASRESPSTWSS LAWVAAAGPSSQAWRAPKASASLTPAAPKRHML *WKRPQQRRPSAGRSAGWQLLPRVAPPQLCWT* AG*QRAWELGSLYLWSAGTAWRWLGQGRGL*AQH GCLPMPASALRPSHTTTLASPRLWRYWPRQQAL KAVRAASSPSAEQPRCSRPFPALSQP*ASCRGF PTLENTPLGLSRSCWSMECVRRWREFGAQRALHP DLRGRCEDC*PVVPGRTANLR*PPRAAPETNPT AESGAPAPPGPEHPSPAVRCRCPAAGGGGSCGA GPAWGHRHADRRLPQGHDVDFLITHPKEGQEAGL LPRVMCRLQDQGLILYHQHQHSCCESPTRLAQQ SHMDAFERSFCIFRLPQPPGAAVGGSTRPCPSW KAVRVDLVVAPVSQFPFALLGWTGSKLFQRELRR FSRKEKGLWLNSHGLFDPEQKTFFQAASEEDIF RHLGLEYLPPEQRNA*ACLCPPLPLRKLGCPQP GH*MSPGRYAAP*PPPSPLPAKAWLFRRSIVPAG SVEPLLVCCRV**GGSPQCQPHHCVTLGLLLAS PWLTFLPWMGCEWGPHRGAVVGPAVMNGKWSLM CSVSLVALQLTVAPAGHPAQNAQKRTMHTCTAFE SHELEAVVRASKEPTVWCAVGIWRGRGPG*ELG GR*FSTMGLQYCSAPSQALCFCL*GLFSAQCLK NRSYPATPVAEPFNGSKP*VHT*PLALASLQPHW PRAA*LTGFLFDAPRPPCGQLPPLLTEAEALRW AGHGCPPRPSYPQAHSGLRFCPGHVIYLEWALP FFRP*IKSHFVRRGFPRPLITLKNILKTCSKVDS WGAVFAHVGHTRSIPRTLPMLSYCGHVLLHPHP LSGATSL*FCFL*NVM*IEPNYI*PLDWPLPTA *CL*RSCRLLGMLSL*FVPCHGCST

Frame 1

PASVSLTGASFRLARSFPLRSLRAAGGCRPNAP QTAASAGRVP*RRCRFLHAALDALPGSRHLPGR ASHGSQPPGLPHRPGALQRLPRP*RLQLRSDTCC DGRDLSRGGRQLAGAQDGSCSPGLHPPSSAGHK LVNREPGSWAACTCGVPAPPGGGWAKEGASEPS MDACLCLPAPYAPHTPQHWPLRGSGDTGRGSRL* RQ*GPPPHLLQSSLGAQGPSQPCHNPEPAAGAS PLWRTLL*GCPGAAGAWSV*GGGESSALRELFT QIFGVGVKTADRWYREGLRTLDDLREQPQKLTQQ QKAGLQHHQDLSTPVLRSDVDALQQVVEEAVGQ ALPGATVTLTGGFRRAMTWTSSSPTPRRVRRRG CCLE*CAACRTRASSCTTSTSTAAVSPLPAWPNR ATWTLLREVSAFSAYHNLQGLLWGDPRGPAHPG RP*EWTW*LHPSASSLSPCSVGLAPSFSSGSCA ASAGRRRACG*TAMGCLTRSRRHFSKRLQRKTSS DTWALSTFLQSRETPEPACVPHFHSGNWAAPNL ATECLQADMLPPDPHLHPSPPRPGSSGGQLCLQ DQLSPCWCAAGCDEVGALSASLITV*PWVCS*PP HGSRSCPGWDVSGAHIVELWWGLQS*MASGP*C AVSH*LHCS*LWLLQGTLPRMPRREPCIPALHL RAMSWRLWFVPARSLLSGVL*ASGEGEGLGRSWE EDNFQLWGFSTAAPRARLCASAFKACSQHNVSK IGHILPLPSQSPLMVPNPKSTHSPWLWHLSSPT GPELLDSPASYLMHPGPLVANSLPFSLRQKH*GG LDMGALHVPHIPRHTLASGFALAMSSTWSGPSP SSGLESKATLLGEDFPDHSSH*KIF*KHAVKLT LGVQFLHT*GTPGPSPEHFPCCPIVVMCFSTPTH LAVLHPYSFAFSRMSCK*NQTIFNLWTGLSPQP NAFDVPAGCWVC*VCNLFHAMGVLQ

Frame 2

PLPSASLGLPSVSLGVSLCVRSALLEAVVPMLP KRRRARVGSPSGDAASSTPPSTRFPGVAIYLVE PRMGRSRRAFLTGLARSKGFRVLDACSSEATHV VMEETSAEEAVSWQERRMAAAPPGCTPPALLDIS WLTESLGAGQPVPVECRHRLEVAGPRKGPLSPA WMPAYACQRPTPLTHHNTGLSEALEILAEAAGF EGSEGRLLTFCRAASVLKALPSPVTTLSQLQGLP HFGEHSSRVVQELLEHGVCEEVERVRRSESSSP RSSGSV*RLLTGGTGKDCEP*MTSESSPRN*PN SRKRGSSTTRT*APQSCGPM*MPCSRWWRKLWGR PCLGPPSR*PAASAGP*RGLPHHPPQGGSGGGA AA*SDVPPAGPGPHPVPPAPAQLL*VPYPPGPT EPHGRF*EKFLHFPPTTTSRGCCGGIHEALPILE GRESGLGSCTRQPVPFRPARLDWLQAFPAGAAP LQPEGEGPVAEQPWAV*PGAEDIFPSGFRGRHL QTPGP*VPSSRAEKRLSLPVSPTSTQEIGLPPTW PLNVSRQICCPLTPTFTPPRQGLALPEVNCACR IS*APAGVLQGVMRWEPSVPASSLCDPGSALSL PMAHVPALDGM*VGPTSWSCGGACSHEWQVVPDV QCLISCTAVNCGSCRAPCPECPEENHAYLHCI* EP*AGGCGSCQQGAYCLVCCRHLERERAWVGAG RKIIFNYGASVLQRPEPGSVLLPLRPVLSTMSQK *VISCHSRRRAL*WFQTLSPHIAPGSGISPAPL APSCLTHRLPI*CTQAPLWPTPSPSH*GRSTEV GWTWVPSTSLISPGTLWPQVLPWPCHLPGVGPPL LQALNQKPLC*ARISQTTHHIKKYFENMQ*S*L LGCSFCTRRAHQVHPQNTSHAVLLWSCASPPPP T*RCYIPIVLLSLECHVNRTKLYLTFGLASPHSL MPLTFLQVAGYVEFVICSMPWVFY

Sequence POLM.fAug05

Frame 0

Frame 1

Frame 2

Sequence POLM.aAug05

Frame 0

Frame 1

Frame 2

Sequence POLM.bAug05

Frame 0

Frame 1

Frame 2

Sequence POLM.cAug05

Frame 0

Frame 1

Frame 2

Sequence POLM.dAug05

Frame 0

Frame 1

Frame 2

Sequence POLM.eAug05

Frame 0

Frame 1

Frame 2

Sequence POLM.hAug05

Frame 0

Frame 1

Frame 2

Sequence POLM.iAug05

Frame 0

Frame 1

Frame 2