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Sequences

Sequence MCL1.bAug05

CCGGCCCCGGCCCCGGCCCCGGCCCCGCCCCGGCCCGGCCGGGCAGCTGGTAGGTGCCGTGCGCAACCCTCCGGAAGCTGCCGCCCCTTTCCCCTTTTAT GGGAATACTTTTTTTAAAAAAAAAGAGTTCGCTGGCGCCACCCCGTAGGACTGGCCGCCCTAAAACCGTGATAAAGGAGCTGCTCGCCACTTCTCACTTC CGCTTCCTTCCAGTAAGGAGTCGGGGTCTTCCCCAGTTTTCTCAGCCAGGCGGCGGCGGCGACTGGCAATGTTTGGCCTCAAAAGAAACGCGGTAATCGG ACTCAACCTCTACTGTGGGGGGGCCGGCTTGGGGGCCGGCAGCGGCGGCGCCACCCGCCCGGGAGGGCGACTTTTGGCTACGGAGAAGGAGGCCTCGGCC CGGCGAGAGATAGGGGGAGGGGAGGCCGGCGCGGTGATTGGCGGAAGCGCCGGCGCAAGCCCCCCGTCCACCCTCACGCCAGACTCCCGGAGGGTCGCGC GGCCGCCGCCCATTGGCGCCGAGGTCCCCGACGTCACCGCGACCCCCGCGAGGCTGCTTTTCTTCGCGCCCACCCGCCGCGCGGCGCCGCTTGAGGAGAT GGAAGCCCCGGCCGCTGACGCCATCATGTCGCCCGAAGAGGAGCTGGACGGGTACGAGCCGGAGCCTCTCGGGAAGCGGCCGGCTGTCCTGCCGCTGCTG GAGTTGGTCGGGGAATCTGGTAATAACACCAGTACGGACGGGTCACTACCCTCGACGCCGCCGCCAGCAGAGGAGGAGGAGGACGAGTTGTACCGGCAGT CGCTGGAGATTATCTCTCGGTACCTTCGGGAGCAGGCCACCGGCGCCAAGGACACAAAGCCAATGGGCAGGTCTGGGGCCACCAGCAGGAAGGCGCTGGA GACCTTACGACGGGTTGGGGATGGCGTGCAGCGCAACCACGAGACGGCCTTCCAAGGATGGGTTTGTGGAGTTCTTCCATGTAGAGGACCTAGAAGGTGG CATCAGGAATGTGCTGCTGGCTTTTGCAGGTGTTGCTGGAGTAGGAGCTGGTTTGGCATATCTAATAAGATAGCCTTACTGTAAGTGCAATAGTTGACTT TTAACCAACCACCACCACCACCAAAACCAGTTTATGCAGTTGGACTCCAAGCTGTAACTTCCTAGAGTTGCACCCTAGCAACCTAGCCAGAAAAGCAAGT GGCAAGAGGATTATGGCTAACAAGAATAAATACATGGGAAGAGTGCTCCCCATTGATTGAAGAGTCACTGTCTGAAAGAAGCAAAGTTCAGTTTCAGCAA CAAACAAACTTTGTTTGGGAAGCTATGGAGGAGGACTTTTAGATTTAGTGAAGATGGTAGGGTGGAAAGACTTAATTTCCTTGTTGAGAACAGGAAAGTG GCCAGTAGCCAGGCAAGTCATAGAATTGATTACCCGCCGAATTCATTAATTTACTGTAGTGTTAAGAGAAGCACTAAGAATGCCAGTGACCTGTGTAAAA GTTACAAGTAATAGAACTATGACTGTAAGCCTCAGTACTGTACAAGGGAAGCTTTTCCTCTCTCTAATTAGCTTTCCCAGTATACTTCTTAGAAAGTCCA AGTGTTCAGGACTTTTATACCTGTTATACTTTGGCTTGGTTTCCATGATTCTTACTTTATTAGCCTAGTTTATCACCAATAATACTTGACGGAAGGCTCA GTAATTAGTTATGAATATGGATATCCTCAATTCTTAAGACAGCTTGTAAATGTATTTGTAAAAATTGTATATATTTTTACAGAAAGTCTATTTCTTTGAA ACGAAGGAAGTATCGAATTTACATTAGTTTTTTTCATACCCTTTTGAACTTTGCAACTTCCGTAATTAGGAACCTGTTTCTTACAGCTTTTCTATGCTAA ACTTTGTTCTGTTCAGTTCTAGAGTGTATACAGAACGAATTGATGTGTAACTGTATGCAGACTGGTTGTAGTGGAACAAATCTGATAACTATGCAGGTTT AAATTTTCTTATCTGATTTTGGTAAGTATTCCTTAGGTTTTTCTTTGAAAACCTGGGATTGAGAGGTTGATGAATGGAAATTCTTTCACTTCATTATATG CAAGTTTTCAATAATTAGGTCTAAGTGGAGTTTTAAGGTTACTGATGACTTACAAATAATGGGCTCTGATTGGGCAATACTCATTTGAGTTCCTTCCATT TGACCTAATTTAACTGGTGAAATTTAAAGTGAATTCATGGGCTCATCTTTAAAGCTTTTACTAAAAGATTTTCAGCTGAATGGAACTCATTAGCTGTGTG CATATAAAAAGATCACATCAGGTGGATGGAGAGACATTTGATCCCTTGTTTGCTTAATAAATTATAAAATGATGGCTTGGAAAAGCAGGCTAGTCTAACC ATGGTGCTATTATTAGGCTTGCTTGTTACACACACAGGTCTAAGCCTAGTATGTCAATAAAGCAAATACTTACTGTTTTGTTTCTATTAATGATTCCCAA ACCTTGTTGCAAGTTTTTGCATTGGCATCTTTGGATTTCAGTCTTGATGTTTGTTCTATCAGACTTAACCTTTTATTTCCTGTCCTTCCTTGAAATTGCT GATTGTTCTGCTCCCTCTACAGATATTTATATCAATTCCTACAGCTTTCCCCTGCCATCCCTGAACTCTTTCTAGCCCTTTTAGATTTTGGCACTGTGAA ACCCCTGCTGGAAACCTGAGTGACCCTCCCTCCCCACCAAGAGTCCACAGACCTTTCATCTTTCACGAACTTGATCCTGTTAGCAGGTGGTAATACCATG GGTGCTGTGACACTAACAGTCATTGAGAGGTGGGAGGAAGTCCCTTTTCCTTGGACTGGTATCTTTTCAACTATTGTTTTATCCTGTCTTTGGGGGCAAT GTGTCAAAAGTCCCCTCAGGAATTTTCAGAGGAAAGAACATTTTATGAGGCTTTCTCTAAAGTTTCCTTTGTATAGGAGTATGCTCACTTAAATTTACAG AAAGAGGTGAGCTGTGTTAAACCTCAGAGTTTAAAAGCTACTGATAAACTGAAGAAAGTGTCTATATTGGAACTAGGGTCATTTGAAAGCTTCAGTCTCG GAACATGACCTTTAGTCTGTGGACTCCATTTAAAAATAGGTATGAATAAGATGACTAAGAATGTAATGGGGAAGAACTGCCCTGCCTGCCCATCTCAGAG CCATAAGGTCATCTTTGCTAGAGCTATTTTTACCTATGTATTTATCGTTCTTGATCATAAGCCGCTTATTTATATCATGTATCTCTAAGGACCTAAAAGC ACTTTATGTAGTTTTTAATTAATCTTAAGATCTGGTTACGGTAACTAAAAAAGCCTGTCTGCCAAATCCAGTGGAAACAAGTGCATAGATGTGAATTGGT TTTTAGGGGCCCCACTTCCCAATTCATTAGGTATGACTGTGGAAATACAGACAAGGATCTTAGTTGATATTTTGGGCTTGGGGCAGTGAGGGCTTAGGAC ACCCCAAGTGGTTTGGGAAAGGAGGAGGGGAGTGGTGGGTTTATAGGGGGAGGAGGAGGCAGGTGGTCTAAGTGCTGACTGGCTACGTAGTTCGGGCAAA TCCTCCAAAAGGGAAAGGGAGGATTTGCTTAGAAGGATGGCGCTCCCAGTGACTACTTTTTGACTTCTGTTTGTCTTACGCTTCTCTCAGGGAAAAACAT GCAGTCCTCTAGTGTTTCATGTACATTCTGTGGGGGGTGAACACCTTGGTTCTGGTTAAACAGCTGTACTTTTGATAGCTGTGCCAGGAAGGGTTAGGAC CAACTACAAATTAATGTTGGTTGTCAAATGTAGTGTGTTTCCCTAACTTTCTGTTTTTCCTGAGAAAAAAAAATAAATCTTTTATTCAAATACAGGGTG

MCL1.F1 (forward primer)
MCL1.R1 (reverse primer)
Single exon skipping

Frame 0

PAPAPAPAPPRPGRAAGRCRAQPSGSCRPFPLL WEYFF*KKRVRWRHPVGLAALKP**RSCSPLLTS ASFQ*GVGVFPSFLSQAAAATGNVWPQKKRGNR TQPLLWGGRLGGRQRRRHPPGRATFGYGEGGLG PARDRGRGGRRGDWRKRRRKPPVHPHARLPEGRA AAAHWRRGPRRHRDPREAAFLRAHPPRGAA*GD GSPGR*RHHVARRGAGRVRAGASREAAGCPAAA GVGRGIW**HQYGRVTTLDAAASRGGGGRVVPAV AGDYLSVPSGAGHRRQGHKANGQVWGHQQEGAG DLTTGWGWRAAQPRDGLPRMGLWSSSM*RT*KV ASGMCCWLLQVLLE*ELVWHI**DSLTVSAIVDF *PTTTTTKTSLCSWTPSCNFLELHPSNLARKAS GKRIMANKNKYMGRVLPID*RVTV*KKQSSVSA TNKLCLGSYGGGLLDLVKMVGWKDLISLLRTGKW PVARQVIELITRRIH*FTVVLREALRMPVTCVK VTSNRTMTVSLSTVQGKLFLSLISFPSILLRKS KCSGLLYLLYFGLVSMILTLLA*FITNNT*RKAQ *LVMNMDILNS*DSL*MYL*KLYIFLQKVYFFE TKEVSNLH*FFSYPFELCNFRN*EPVSYSFSML NFVLFSSRVYTERIDV*LYADWL*WNKSDNYAGL NFLI*FW*VFLRFFFENLGLRG**MEILSLHYM QVFNN*V*VEF*GY**LTNNGL*LGNTHLSSFH LT*FNW*NLK*IHGLIFKAFTKRFSAEWNSLAVC I*KDHIRWMERHLIPCLLNKL*NDGLEKQASLT MVLLLGLLVTHTGLSLVCQ*SKYLLFCFY**FP NLVASFCIGIFGFQS*CLFYQT*PFISCPSLKLL IVLLPLQIFISIPTAFPCHP*TLSSPFRFWHCE TPAGNLSDPPSPPRVHRPFIFHELDPVSRW*YH GCCDTNSH*EVGGSPFSLDWYLFNYCFILSLGAM CQKSPQEFSEERTFYEAFSKVSFV*EYAHLNLQ KEVSCVKPQSLKATDKLKKVSILELGSFESFSL GT*PLVCGLHLKIGMNKMTKNVMGKNCPACPSQS HKVIFARAIFTYVFIVLDHKPLIYIMYL*GPKS TLCSF*LILRSGYGN*KSLSAKSSGNKCIDVNW FLGAPLPNSLGMTVEIQTRILVDILGLGQ*GLRT PQVVWERRRGVVGL*GEEEAGGLSADWLRSSGK SSKREREDLLRRMALPVTTF*LLFVLRFSQGKT CSPLVFHVHSVGGEHLGSG*TAVLLIAVPGRVRT NYKLMLVVKCSVFP*LSVFPEKKK*IFYSNTG

Frame 1

RPRPRPRPRPGPAGQLVGAVRNPPEAAAPFPFY GNTFFKKKEFAGATP*DWPP*NRDKGAARHFSL PLPSSKESGSSPVFSARRRRRLAMFGLKRNAVIG LNLYCGGAGLGAGSGGATRPGGRLLATEKEASA RREIGGGEAGAVIGGSAGASPPSTLTPDSRRVA RPPPIGAEVPDVTATPARLLFFAPTRRAAPLEEM EAPAADAIMSPEEELDGYEPEPLGKRPAVLPLL ELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQ SLEIISRYLREQATGAKDTKPMGRSGATSRKALE TLRRVGDGVQRNHETAFQGWVCGVLPCRGPRRW HQECAAGFCRCCWSRSWFGISNKIALL*VQ*LT FNQPPPPPKPVYAVGLQAVTS*SCTLAT*PEKQV ARGLWLTRINTWEECSPLIEESLSERSKVQFQQ QTNFVWEAMEEDF*I**RW*GGKT*FPC*EQES GQ*PGKS*N*LPAEFINLL*C*EKH*ECQ*PV*K LQVIEL*L*ASVLYKGSFSSL*LAFPVYFLESP SVQDFYTCYTLAWFP*FLLY*PSLSPIILDGRL SN*L*IWISSILKTACKCICKNCIYFYRKSISLK RRKYRIYISFFHTLLNFATSVIRNLFLTAFLC* TLFCSVLECIQNELMCNCMQTGCSGTNLITMQV *IFLSDFGKYSLGFSLKTWD*EVDEWKFFHFIIC KFSIIRSKWSFKVTDDLQIMGSDWAILI*VPSI *PNLTGEI*SEFMGSSLKLLLKDFQLNGTH*LC AYKKITSGGWRDI*SLVCLINYKMMAWKSRLV*P WCYY*ACLLHTQV*A*YVNKANTYCFVSINDSQ TLLQVFALASLDFSLDVCSIRLNLLFPVLP*NC *LFCSLYRYLYQFLQLSPAIPELFLALLDFGTVK PLLET*VTLPPHQESTDLSSFTNLILLAGGNTM GAVTLTVIERWEEVPFPWTGIFSTIVLSCLWGQ CVKSPLRNFQRKEHFMRLSLKFPLYRSMLT*IYR KR*AVLNLRV*KLLIN*RKCLYWN*GHLKASVS EHDL*SVDSI*K*V*IR*LRM*WGRTALPAHLR AIRSSLLELFLPMYLSFLIISRLFISCISKDLKA LYVVFN*S*DLVTVTKKACLPNPVETSA*M*IG F*GPHFPIH*V*LWKYRQGS*LIFWAWGSEGLG HPKWFGKGGGEWWVYRGRRRQVV*VLTGYVVRAN PPKGKGRICLEGWRSQ*LLFDFCLSYASLREKH AVL*CFMYILWGVNTLVLVKQLYF**LCQEGLG PTTN*CWLSNVVCFPNFLFFLRKKNKSFIQIQG

Frame 2

GPGPGPGPAPARPGSW*VPCATLRKLPPLSPFM GILFLKKKSSLAPPRRTGRPKTVIKELLATSHF RFLPVRSRGLPQFSQPGGGGDWQCLASKETR*S DSTSTVGGPAWGPAAAAPPAREGDFWLRRRRPRP GER*GEGRPAR*LAEAPAQAPRPPSRQTPGGSR GRRPLAPRSPTSPRPPRGCFSSRPPAARRRLRR WKPRPLTPSCRPKRSWTGTSRSLSGSGRLSCRCW SWSGNLVITPVRTGHYPRRRRQQRRRRTSCTGS RWRLSLGTFGSRPPAPRTQSQWAGLGPPAGRRW RPYDGLGMACSATTRRPSKDGFVEFFHVEDLEGG IRNVLLAFAGVAGVGAGLAYLIR*PYCKCNS*L LTNHHHHQNQFMQLDSKL*LPRVAP*QPSQKSK WQEDYG*QE*IHGKSAPH*LKSHCLKEAKFSFSN KQTLFGKLWRRTFRFSEDGRVERLNFLVENRKV ASSQASHRIDYPPNSLIYCSVKRSTKNASDLCK SYK**NYDCKPQYCTREAFPLSN*LSQYTS*KVQ VFRTFIPVILWLGFHDSYFISLVYHQ*YLTEGS VISYEYGYPQFLRQLVNVFVKIVYIFTESLFL* NEGSIEFTLVFFIPF*TLQLP*LGTCFLQLFYAK LCSVQF*SVYRTN*CVTVCRLVVVEQI**LCRF KFSYLILVSIP*VFL*KPGIERLMNGNSFTSLY ASFQ*LGLSGVLRLLMTYK*WALIGQYSFEFLPF DLI*LVKFKVNSWAHL*SFY*KIFS*MELISCV HIKRSHQVDGETFDPLFA**IIK*WLGKAG*SN HGAIIRLACYTHRSKPSMSIKQILTVLFLLMIPK PCCKFLHWHLWISVLMFVLSDLTFYFLSFLEIA DCSAPSTDIYINSYSFPLPSLNSF*PF*ILAL* NPCWKPE*PSLPTKSPQTFHLSRT*SC*QVVIPW VL*H*QSLRGGRKSLFLGLVSFQLLFYPVFGGN VSKVPSGIFRGKNIL*GFL*SFLCIGVCSLKFT ERGELC*TSEFKSY**TEESVYIGTRVI*KLQSR NMTFSLWTPFKNRYE*DD*ECNGEELPCLPISE P*GHLC*SYFYLCIYRS*S*AAYLYHVSLRT*K HFM*FLINLKIWLR*LKKPVCQIQWKQVHRCELV FRGPTSQFIRYDCGNTDKDLS*YFGLGAVRA*D TPSGLGKEEGSGGFIGGGGGRWSKC*LAT*FGQ ILQKGKGGFA*KDGAPSDYFLTSVCLTLLSGKNM QSSSVSCTFCGG*TPWFWLNSCTFDSCARKG*D QLQINVGCQM*CVSLTFCFS*EKKINLLFKYRV

Sequence MCL1.aAug05

CCGGCCCCGGCCCCGGCCCCGGCCCCGCCCCGGCCCGGCCGGGCAGCTGGTAGGTGCCGTGCGCAACCCTCCGGAAGCTGCCGCCCCTTTCCCCTTTTAT GGGAATACTTTTTTTAAAAAAAAAGAGTTCGCTGGCGCCACCCCGTAGGACTGGCCGCCCTAAAACCGTGATAAAGGAGCTGCTCGCCACTTCTCACTTC CGCTTCCTTCCAGTAAGGAGTCGGGGTCTTCCCCAGTTTTCTCAGCCAGGCGGCGGCGGCGACTGGCAATGTTTGGCCTCAAAAGAAACGCGGTAATCGG ACTCAACCTCTACTGTGGGGGGGCCGGCTTGGGGGCCGGCAGCGGCGGCGCCACCCGCCCGGGAGGGCGACTTTTGGCTACGGAGAAGGAGGCCTCGGCC CGGCGAGAGATAGGGGGAGGGGAGGCCGGCGCGGTGATTGGCGGAAGCGCCGGCGCAAGCCCCCCGTCCACCCTCACGCCAGACTCCCGGAGGGTCGCGC GGCCGCCGCCCATTGGCGCCGAGGTCCCCGACGTCACCGCGACCCCCGCGAGGCTGCTTTTCTTCGCGCCCACCCGCCGCGCGGCGCCGCTTGAGGAGAT GGAAGCCCCGGCCGCTGACGCCATCATGTCGCCCGAAGAGGAGCTGGACGGGTACGAGCCGGAGCCTCTCGGGAAGCGGCCGGCTGTCCTGCCGCTGCTG GAGTTGGTCGGGGAATCTGGTAATAACACCAGTACGGACGGGTCACTACCCTCGACGCCGCCGCCAGCAGAGGAGGAGGAGGACGAGTTGTACCGGCAGT CGCTGGAGATTATCTCTCGGTACCTTCGGGAGCAGGCCACCGGCGCCAAGGACACAAAGCCAATGGGCAGGTCTGGGGCCACCAGCAGGAAGGCGCTGGA GACCTTACGACGGGTTGGGGATGGCGTGCAGCGCAACCACGAGACGGCCTTCCAAGGCATGCTTCGGAAACTGGACATCAAAAACGAAGACGATGTGAAA TCGTTGTCTCGAGTGATGATCCATGTTTTCAGCGACGGCGTAACAAACTGGGGCAGGATTGTGACTCTCATTTCTTTTGGTGCCTTTGTGGCTAAACACT TGAAGACCATAAACCAAGAAAGCTGCATCGAACCATTAGCAGAAAGTATCACAGACGTTCTCGTAAGGACAAAACGGGACTGGCTAGTTAAACAAAGAGG CTGGGATGGGTTTGTGGAGTTCTTCCATGTAGAGGACCTAGAAGGTGGCATCAGGAATGTGCTGCTGGCTTTTGCAGGTGTTGCTGGAGTAGGAGCTGGT TTGGCATATCTAATAAGATAGCCTTACTGTAAGTGCAATAGTTGACTTTTAACCAACCACCACCACCACCAAAACCAGTTTATGCAGTTGGACTCCAAGC TGTAACTTCCTAGAGTTGCACCCTAGCAACCTAGCCAGAAAAGCAAGTGGCAAGAGGATTATGGCTAACAAGAATAAATACATGGGAAGAGTGCTCCCCA TTGATTGAAGAGTCACTGTCTGAAAGAAGCAAAGTTCAGTTTCAGCAACAAACAAACTTTGTTTGGGAAGCTATGGAGGAGGACTTTTAGATTTAGTGAA GATGGTAGGGTGGAAAGACTTAATTTCCTTGTTGAGAACAGGAAAGTGGCCAGTAGCCAGGCAAGTCATAGAATTGATTACCCGCCGAATTCATTAATTT ACTGTAGTGTTAAGAGAAGCACTAAGAATGCCAGTGACCTGTGTAAAAGTTACAAGTAATAGAACTATGACTGTAAGCCTCAGTACTGTACAAGGGAAGC TTTTCCTCTCTCTAATTAGCTTTCCCAGTATACTTCTTAGAAAGTCCAAGTGTTCAGGACTTTTATACCTGTTATACTTTGGCTTGGTTTCCATGATTCT TACTTTATTAGCCTAGTTTATCACCAATAATACTTGACGGAAGGCTCAGTAATTAGTTATGAATATGGATATCCTCAATTCTTAAGACAGCTTGTAAATG TATTTGTAAAAATTGTATATATTTTTACAGAAAGTCTATTTCTTTGAAACGAAGGAAGTATCGAATTTACATTAGTTTTTTTCATACCCTTTTGAACTTT GCAACTTCCGTAATTAGGAACCTGTTTCTTACAGCTTTTCTATGCTAAACTTTGTTCTGTTCAGTTCTAGAGTGTATACAGAACGAATTGATGTGTAACT GTATGCAGACTGGTTGTAGTGGAACAAATCTGATAACTATGCAGGTTTAAATTTTCTTATCTGATTTTGGTAAGTATTCCTTAGGTTTTTCTTTGAAAAC CTGGGATTGAGAGGTTGATGAATGGAAATTCTTTCACTTCATTATATGCAAGTTTTCAATAATTAGGTCTAAGTGGAGTTTTAAGGTTACTGATGACTTA CAAATAATGGGCTCTGATTGGGCAATACTCATTTGAGTTCCTTCCATTTGACCTAATTTAACTGGTGAAATTTAAAGTGAATTCATGGGCTCATCTTTAA AGCTTTTACTAAAAGATTTTCAGCTGAATGGAACTCATTAGCTGTGTGCATATAAAAAGATCACATCAGGTGGATGGAGAGACATTTGATCCCTTGTTTG CTTAATAAATTATAAAATGATGGCTTGGAAAAGCAGGCTAGTCTAACCATGGTGCTATTATTAGGCTTGCTTGTTACACACACAGGTCTAAGCCTAGTAT GTCAATAAAGCAAATACTTACTGTTTTGTTTCTATTAATGATTCCCAAACCTTGTTGCAAGTTTTTGCATTGGCATCTTTGGATTTCAGTCTTGATGTTT GTTCTATCAGACTTAACCTTTTATTTCCTGTCCTTCCTTGAAATTGCTGATTGTTCTGCTCCCTCTACAGATATTTATATCAATTCCTACAGCTTTCCCC TGCCATCCCTGAACTCTTTCTAGCCCTTTTAGATTTTGGCACTGTGAAACCCCTGCTGGAAACCTGAGTGACCCTCCCTCCCCACCAAGAGTCCACAGAC CTTTCATCTTTCACGAACTTGATCCTGTTAGCAGGTGGTAATACCATGGGTGCTGTGACACTAACAGTCATTGAGAGGTGGGAGGAAGTCCCTTTTCCTT GGACTGGTATCTTTTCAACTATTGTTTTATCCTGTCTTTGGGGGCAATGTGTCAAAAGTCCCCTCAGGAATTTTCAGAGGAAAGAACATTTTATGAGGCT TTCTCTAAAGTTTCCTTTGTATAGGAGTATGCTCACTTAAATTTACAGAAAGAGGTGAGCTGTGTTAAACCTCAGAGTTTAAAAGCTACTGATAAACTGA AGAAAGTGTCTATATTGGAACTAGGGTCATTTGAAAGCTTCAGTCTCGGAACATGACCTTTAGTCTGTGGACTCCATTTAAAAATAGGTATGAATAAGAT GACTAAGAATGTAATGGGGAAGAACTGCCCTGCCTGCCCATCTCAGAGCCATAAGGTCATCTTTGCTAGAGCTATTTTTACCTATGTATTTATCGTTCTT GATCATAAGCCGCTTATTTATATCATGTATCTCTAAGGACCTAAAAGCACTTTATGTAGTTTTTAATTAATCTTAAGATCTGGTTACGGTAACTAAAAAA GCCTGTCTGCCAAATCCAGTGGAAACAAGTGCATAGATGTGAATTGGTTTTTAGGGGCCCCACTTCCCAATTCATTAGGTATGACTGTGGAAATACAGAC AAGGATCTTAGTTGATATTTTGGGCTTGGGGCAGTGAGGGCTTAGGACACCCCAAGTGGTTTGGGAAAGGAGGAGGGGAGTGGTGGGTTTATAGGGGGAG GAGGAGGCAGGTGGTCTAAGTGCTGACTGGCTACGTAGTTCGGGCAAATCCTCCAAAAGGGAAAGGGAGGATTTGCTTAGAAGGATGGCGCTCCCAGTGA CTACTTTTTGACTTCTGTTTGTCTTACGCTTCTCTCAGGGAAAAACATGCAGTCCTCTAGTGTTTCATGTACATTCTGTGGGGGGTGAACACCTTGGTTC TGGTTAAACAGCTGTACTTTTGATAGCTGTGCCAGGAAGGGTTAGGACCAACTACAAATTAATGTTGGTTGTCAAATGTAGTGTGTTTCCCTAACTTTCT GTTTTTCCTGAGAAAAAAAAATAAATCTTTTATTCAAATACAGGGTG

MCL1.F1 (forward primer)
MCL1.R1 (reverse primer)
Single exon skipping

Frame 0

PAPAPAPAPPRPGRAAGRCRAQPSGSCRPFPLL WEYFF*KKRVRWRHPVGLAALKP**RSCSPLLTS ASFQ*GVGVFPSFLSQAAAATGNVWPQKKRGNR TQPLLWGGRLGGRQRRRHPPGRATFGYGEGGLG PARDRGRGGRRGDWRKRRRKPPVHPHARLPEGRA AAAHWRRGPRRHRDPREAAFLRAHPPRGAA*GD GSPGR*RHHVARRGAGRVRAGASREAAGCPAAA GVGRGIW**HQYGRVTTLDAAASRGGGGRVVPAV AGDYLSVPSGAGHRRQGHKANGQVWGHQQEGAG DLTTGWGWRAAQPRDGLPRHASETGHQKRRRCE IVVSSDDPCFQRRRNKLGQDCDSHFFWCLCG*TL EDHKPRKLHRTISRKYHRRSRKDKTGLAS*TKR LGWVCGVLPCRGPRRWHQECAAGFCRCCWSRSW FGISNKIALL*VQ*LTFNQPPPPPKPVYAVGLQA VTS*SCTLAT*PEKQVARGLWLTRINTWEECSP LIEESLSERSKVQFQQQTNFVWEAMEEDF*I** RW*GGKT*FPC*EQESGQ*PGKS*N*LPAEFINL L*C*EKH*ECQ*PV*KLQVIEL*L*ASVLYKGS FSSL*LAFPVYFLESPSVQDFYTCYTLAWFP*F LLY*PSLSPIILDGRLSN*L*IWISSILKTACKC ICKNCIYFYRKSISLKRRKYRIYISFFHTLLNF ATSVIRNLFLTAFLC*TLFCSVLECIQNELMCN CMQTGCSGTNLITMQV*IFLSDFGKYSLGFSLKT WD*EVDEWKFFHFIICKFSIIRSKWSFKVTDDL QIMGSDWAILI*VPSI*PNLTGEI*SEFMGSSL KLLLKDFQLNGTH*LCAYKKITSGGWRDI*SLVC LINYKMMAWKSRLV*PWCYY*ACLLHTQV*A*Y VNKANTYCFVSINDSQTLLQVFALASLDFSLDV CSIRLNLLFPVLP*NC*LFCSLYRYLYQFLQLSP AIPELFLALLDFGTVKPLLET*VTLPPHQESTD LSSFTNLILLAGGNTMGAVTLTVIERWEEVPFP WTGIFSTIVLSCLWGQCVKSPLRNFQRKEHFMRL SLKFPLYRSMLT*IYRKR*AVLNLRV*KLLIN* RKCLYWN*GHLKASVSEHDL*SVDSI*K*V*IR *LRM*WGRTALPAHLRAIRSSLLELFLPMYLSFL IISRLFISCISKDLKALYVVFN*S*DLVTVTKK ACLPNPVETSA*M*IGF*GPHFPIH*V*LWKYR QGS*LIFWAWGSEGLGHPKWFGKGGGEWWVYRGR RRQVV*VLTGYVVRANPPKGKGRICLEGWRSQ* LLFDFCLSYASLREKHAVL*CFMYILWGVNTLV LVKQLYF**LCQEGLGPTTN*CWLSNVVCFPNFL FFLRKKNKSFIQIQG

Frame 1

RPRPRPRPRPGPAGQLVGAVRNPPEAAAPFPFY GNTFFKKKEFAGATP*DWPP*NRDKGAARHFSL PLPSSKESGSSPVFSARRRRRLAMFGLKRNAVIG LNLYCGGAGLGAGSGGATRPGGRLLATEKEASA RREIGGGEAGAVIGGSAGASPPSTLTPDSRRVA RPPPIGAEVPDVTATPARLLFFAPTRRAAPLEEM EAPAADAIMSPEEELDGYEPEPLGKRPAVLPLL ELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQ SLEIISRYLREQATGAKDTKPMGRSGATSRKALE TLRRVGDGVQRNHETAFQGMLRKLDIKNEDDVK SLSRVMIHVFSDGVTNWGRIVTLISFGAFVAKH LKTINQESCIEPLAESITDVLVRTKRDWLVKQRG WDGFVEFFHVEDLEGGIRNVLLAFAGVAGVGAG LAYLIR*PYCKCNS*LLTNHHHHQNQFMQLDSK L*LPRVAP*QPSQKSKWQEDYG*QE*IHGKSAPH *LKSHCLKEAKFSFSNKQTLFGKLWRRTFRFSE DGRVERLNFLVENRKVASSQASHRIDYPPNSLI YCSVKRSTKNASDLCKSYK**NYDCKPQYCTREA FPLSN*LSQYTS*KVQVFRTFIPVILWLGFHDS YFISLVYHQ*YLTEGSVISYEYGYPQFLRQLVN VFVKIVYIFTESLFL*NEGSIEFTLVFFIPF*TL QLP*LGTCFLQLFYAKLCSVQF*SVYRTN*CVT VCRLVVVEQI**LCRFKFSYLILVSIP*VFL*K PGIERLMNGNSFTSLYASFQ*LGLSGVLRLLMTY K*WALIGQYSFEFLPFDLI*LVKFKVNSWAHL* SFY*KIFS*MELISCVHIKRSHQVDGETFDPLF A**IIK*WLGKAG*SNHGAIIRLACYTHRSKPSM SIKQILTVLFLLMIPKPCCKFLHWHLWISVLMF VLSDLTFYFLSFLEIADCSAPSTDIYINSYSFP LPSLNSF*PF*ILAL*NPCWKPE*PSLPTKSPQT FHLSRT*SC*QVVIPWVL*H*QSLRGGRKSLFL GLVSFQLLFYPVFGGNVSKVPSGIFRGKNIL*G FL*SFLCIGVCSLKFTERGELC*TSEFKSY**TE ESVYIGTRVI*KLQSRNMTFSLWTPFKNRYE*D D*ECNGEELPCLPISEP*GHLC*SYFYLCIYRS *S*AAYLYHVSLRT*KHFM*FLINLKIWLR*LKK PVCQIQWKQVHRCELVFRGPTSQFIRYDCGNTD KDLS*YFGLGAVRA*DTPSGLGKEEGSGGFIGG GGGRWSKC*LAT*FGQILQKGKGGFA*KDGAPSD YFLTSVCLTLLSGKNMQSSSVSCTFCGG*TPWF WLNSCTFDSCARKG*DQLQINVGCQM*CVSLTF CFS*EKKINLLFKYRV

Frame 2

GPGPGPGPAPARPGSW*VPCATLRKLPPLSPFM GILFLKKKSSLAPPRRTGRPKTVIKELLATSHF RFLPVRSRGLPQFSQPGGGGDWQCLASKETR*S DSTSTVGGPAWGPAAAAPPAREGDFWLRRRRPRP GER*GEGRPAR*LAEAPAQAPRPPSRQTPGGSR GRRPLAPRSPTSPRPPRGCFSSRPPAARRRLRR WKPRPLTPSCRPKRSWTGTSRSLSGSGRLSCRCW SWSGNLVITPVRTGHYPRRRRQQRRRRTSCTGS RWRLSLGTFGSRPPAPRTQSQWAGLGPPAGRRW RPYDGLGMACSATTRRPSKACFGNWTSKTKTM*N RCLE**SMFSATA*QTGAGL*LSFLLVPLWLNT *RP*TKKAASNH*QKVSQTFS*GQNGTG*LNKE AGMGLWSSSM*RT*KVASGMCCWLLQVLLE*ELV WHI**DSLTVSAIVDF*PTTTTTKTSLCSWTPS CNFLELHPSNLARKASGKRIMANKNKYMGRVLP ID*RVTV*KKQSSVSATNKLCLGSYGGGLLDLVK MVGWKDLISLLRTGKWPVARQVIELITRRIH*F TVVLREALRMPVTCVKVTSNRTMTVSLSTVQGK LFLSLISFPSILLRKSKCSGLLYLLYFGLVSMIL TLLA*FITNNT*RKAQ*LVMNMDILNS*DSL*M YL*KLYIFLQKVYFFETKEVSNLH*FFSYPFEL CNFRN*EPVSYSFSMLNFVLFSSRVYTERIDV*L YADWL*WNKSDNYAGLNFLI*FW*VFLRFFFEN LGLRG**MEILSLHYMQVFNN*V*VEF*GY**L TNNGL*LGNTHLSSFHLT*FNW*NLK*IHGLIFK AFTKRFSAEWNSLAVCI*KDHIRWMERHLIPCL LNKL*NDGLEKQASLTMVLLLGLLVTHTGLSLV CQ*SKYLLFCFY**FPNLVASFCIGIFGFQS*CL FYQT*PFISCPSLKLLIVLLPLQIFISIPTAFP CHP*TLSSPFRFWHCETPAGNLSDPPSPPRVHR PFIFHELDPVSRW*YHGCCDTNSH*EVGGSPFSL DWYLFNYCFILSLGAMCQKSPQEFSEERTFYEA FSKVSFV*EYAHLNLQKEVSCVKPQSLKATDKL KKVSILELGSFESFSLGT*PLVCGLHLKIGMNKM TKNVMGKNCPACPSQSHKVIFARAIFTYVFIVL DHKPLIYIMYL*GPKSTLCSF*LILRSGYGN*K SLSAKSSGNKCIDVNWFLGAPLPNSLGMTVEIQT RILVDILGLGQ*GLRTPQVVWERRRGVVGL*GE EEAGGLSADWLRSSGKSSKREREDLLRRMALPV TTF*LLFVLRFSQGKTCSPLVFHVHSVGGEHLGS G*TAVLLIAVPGRVRTNYKLMLVVKCSVFP*LS VFPEKKK*IFYSNTG