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Sequences

Sequence LGALS9.dAug05

TGTTTGTTAAATTATATGTAGGGACAGGGTCTTCCAACCTTTCCCGAGCTGGTCTCAAGCTCCTGGGCTGAAGTGATCCTCCTGCCTCAGCCTCTCAAAG TGCTGGTATTATAGGCATAAGCCACCACGCCCTGCCCATTTTTCTAAGTTGGATTATTTTACACTCCAGTCTGCTGTGAATGAGAGTTCCAGTTGTTGCG TATCGCAATCAATTTGTGATTGCCTACAAATGATGTGCCGTGATGAAATTGTCCCTGGTGTCCGAGAGGATCGCAAAGAATTTCCCTTCCTTTCTCTCCT TTCTGCTCCCCTTGTACTGTCTACTCCTTAAACTGGTACTTGATCCAGGAACATAATGGGGATGGACAGTGCAGATGGCACACATGGCCTCAGTCTATTG GAGCATCTCAAAGGAATTAGTCTGCAAATTCTAGTGCATCTAGGTGGAGCACAGGAAGGAAGAGAGGTGCAGACTCACGTATGGCCCTGGGGCACCTGCC ATCCTCTCGCACCCGTACAGCCCTTACCTGCCAGCACTTACCCTACCCAGCTTGAGTTCTGGGCCTTCTCACCAGCACATCGCTGGGACCTGGTCGACTT CAGGTAAGTTTATTTCATGGCTCCCTCCCCCACGACCTGCACATGCCATATGTTCCTTCCCAGCCCCATGTCACTTGAAATGTTCTATCATGTCCAAGAC TTCTCTCCTCATCCTAAAGTGCAGAAAATAAGGATGGAGAAGAGAAGGAGAAGAGAATCAAGAGAGATTCATTGAGAGGGAGGATCAGAAGCTCCTGGGC ACCCCATACTGTAGAATAAAAATATTCATTATGGTGTTTTTCTTGCAAAATATTCCCCTCTACATAATTATGTTTTTACATGAATAATATAAATTTAAAC AGGAGAAATACTTTTTGAAGAGTCTGAGAATCTTCATAAAGTTCAGGCGGCCACGAATTAGCTGTGTGACCTGGGGCATGCCCCTTAGACTCTGAGCCTC AGCTTCTCTGTATGTAAAATGGGTTGAAGCCGCCCTTCCCCACAAGCACCCTGTGCACAGGCAATGCCCAGCCCCATTATTTTCTGGACTCCGTGGCCAA GCATGCTTAGGACACACAGCCACATACTTCTGGGCAGTGTCATCTGGCAACTTGCTGTCATGTCAGTGTGGTCAAGCATTGTAGACCTCTATGAACCAAA TATGCTTCAGGCTTGGGTTGAGCACAGGAGAGGGAGGAGGGAAAGGTCACTGGGGCTGGGAGTGCCTACTTCCCTCTGTGAGTGTCACCCCAGTCCACCC AGTACCATACCTTTCTCTCTCTGGACCCACTTCCTTTTGCTGCCGGCTCCTCCCCATTGAATAACAGCCAAGTTGCTTTGGTTTCTATTTCTTTGTTAAG TCGTTCCCTCTACAAAGGACTTCCTAGTGGGTGTGAAAGGCAGCGGTGGCCACAGAGGCGGCGGAGAGATGGCCTTCAGCGGTTCCCAGGCTCCCTACCT GAGTCCAGCTGTCCCCTTTTCTGGGACTATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAGG TTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTCCACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGC AGAACGGAAGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGACCTCTGCTTCCTGGTGCAGAGCTCAGATTTCAA GGTGATGGTGAACGGGATCCTCTTCGTGCAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTGCAGCTGTCCTAC ATCAGCTTCCAGCCTCCCGGCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGTGCAGAGCGCCCCTGGACAGATGTTCTCTA CTCCCGCCATCCCACCTATGATGTACCCCCACCCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAGTCCATCCTCCT GTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTGGCCGTGGATGGTCAGCACCTGTTTGAATACTACC ATCGCCTGAGGAACCTGCCCACCATCAACAGACTGGAAGTGGGGGGCGACATCCAGCTGACCCATGTGCAGACATAGGCGGCTTCCTGGCCCTGGGGCCG GGGGCTGGGGTGTGGGGCAGTCTGGGTCCTCTCATCATCCCCACTTCCCAGGCCCAGCCTTTCCAACCCTGCCTGGGATCTGGGCTTTAATGCAGAGGCC ATGTCCTTGTCTGGTCCTGCTTCTGGCTACAGCCACCCTGGAACGGAGAAGGCAGCTGACGGGGATTGCCTTCCTCAGCCGCAGCAGCACCTGGGGCTCC AGCTGCTGGAATCCTACCATCCCAGGAGGCAGGCACAGCCAGGGAGAGGGGAGGAGTGGGCAGTGAAGATGAAGCCCCATGCTCAGTCCCCTCCCATCCC CCACGCAGCTCCACCCCAGTCCCAAGCCACCAGCTGTCTGCTCCTGGTGGGAGGTGGCCTCCTCAGCCCCTCCTCTCTGACCTTTAACCTCACTCTCACC TTGCACCGTGCACCAACCCTTCACCCCTCCTGGAAAGCAGGCCTGATGGCTTCCCACTGGCCTCCACCACCTGACCAGAGTGTTCTCTTCAGAGGACTGG CTCCTTTCCCAGTGTCCTTAAAATAAAGAAATGAAAATGCTTGTTGGCACATTCA

LGALS9.F10 (forward primer)
LGALS9.R6 (reverse primer)
Single exon skipping

Frame 0

CLLNYM*GQGLPTFPELVSSSWAEVILLPQPLK VLVL*A*ATTPCPFF*VGLFYTPVCCE*EFQLLR IAINL*LPTNDVP**NCPWCPRGSQRISLPFSP FCSPCTVYSLNWYLIQEHNGDGQCRWHTWPQSI GASQRN*SANSSASRWSTGRKRGADSRMALGHLP SSRTRTALTCQHLPYPA*VLGLLTSTSLGPGRL QVSLFHGSLPHDLHMPYVPSQPHVT*NVLSCPR LLSSS*SAENKDGEEKEKRIKRDSLRGRIRSSWA PHTVE*KYSLWCFSCKIFPST*LCFYMNNINLN RRNTF*RV*ESS*SSGGHELAV*PGACPLDSEP QLLCM*NGLKPPFPTSTLCTGNAQPHYFLDSVAK HA*DTQPHTSGQCHLATCCHVSVVKHCRPL*TK YASGLG*AQEREEGKVTGAGSAYFPL*VSPQST QYHTFLSLDPLPFAAGSSPLNNSQVALVSISLLS RSLYKGLPSGCERQRWPQRRRRDGLQRFPGSLP ESSCPLFWDYSRRSPGRTSDHCQWDRSQLQWNQ VCCELSDWLQWK*HCLPLQPSV*RWRVRGVQHEA ERKLGARGEEDTHAFPEGDAL*PLLPGAELRFQ GDGERDPLRAVLPPRALPPCGHHLRQWLCAAVL HQLPASRRVACQPGSHYPDSHPHSAERPWTDVLY SRHPTYDVPPPRLSDAFHHHHSGRAVPIQVHPP VRHCPAQCSEVWILCEAHCLKVAVDGQHLFEYY HRLRNLPTINRLEVGGDIQLTHVQT*AASWPWGR GLGCGAVWVLSSSPLPRPSLSNPAWDLGFNAEA MSLSGPASGYSHPGTEKAADGDCLPQPQQHLGL QLLESYHPRRQAQPGRGEEWAVKMKPHAQSPPIP HAAPPQSQATSCLLLVGGGLLSPSSLTFNLTLT LHRAPTLHPSWKAGLMASHWPPPPDQSVLFRGL APFPVSLK*RNENACWHI

Frame 1

VC*IICRDRVFQPFPSWSQAPGLK*SSCLSLSK CWYYRHKPPRPAHFSKLDYFTLQSAVNESSSCC VSQSICDCLQMMCRDEIVPGVREDRKEFPFLSLL SAPLVLSTP*TGT*SRNIMGMDSADGTHGLSLL EHLKGISLQILVHLGGAQEGREVQTHVWPWGTC HPLAPVQPLPASTYPTQLEFWAFSPAHRWDLVDF R*VYFMAPSPTTCTCHMFLPSPMSLEMFYHVQD FSPHPKVQKIRMEKRRRRESREIH*EGGSEAPG HPIL*NKNIHYGVFLAKYSPLHNYVFT*II*I*T GEILFEESENLHKVQAATN*LCDLGHAP*TLSL SFSVCKMG*SRPSPQAPCAQAMPSPIIFWTPWP SMLRTHSHILLGSVIWQLAVMSVWSSIVDLYEPN MLQAWVEHRRGRRERSLGLGVPTSLCECHPSPP STIPFSLWTHFLLLPAPPH*ITAKLLWFLFLC* VVPSTKDFLVGVKGSGGHRGGGEMAFSGSQAPYL SPAVPFSGTIQGGLQDGLQITVNGTVLSSSGTR FAVNFQTGFSGNDIAFHFNPRFEDGGYVVCNTR QNGSWGPEERKTHMPFQKGMPFDLCFLVQSSDFK VMVNGILFVQYFHRVPFHRVDTISVNGSVQLSY ISFQPPGVWPANPAPITQTVIHTVQSAPGQMFS TPAIPPMMYPHPAYPMPFITTILGGLYPSKSILL SGTVLPSAQRCGSCVKLTASRWPWMVSTCLNTT IA*GTCPPSTDWKWGATSS*PMCRHRRLPGPGA GGWGVGQSGSSHHPHFPGPAFPTLPGIWALMQRP CPCLVLLLATATLERRRQLTGIAFLSRSSTWGS SCWNPTIPGGRHSQGEGRSGQ*R*SPMLSPLPS PTQLHPSPKPPAVCSWWEVASSAPPL*PLTSLSP CTVHQPFTPPGKQA*WLPTGLHHLTRVFSSEDW LLSQCP*NKEMKMLVGTF

Frame 2

FVKLYVGTGSSNLSRAGLKLLG*SDPPASASQS AGIIGISHHALPIFLSWIILHSSLL*MRVPVVA YRNQFVIAYK*CAVMKLSLVSERIAKNFPSFLS FLLPLYCLLLKLVLDPGT*WGWTVQMAHMASVYW SISKELVCKF*CI*VEHRKEERCRLTYGPGAPA ILSHPYSPYLPALTLPSLSSGPSHQHIAGTWST SGKFISWLPPPRPAHAICSFPAPCHLKCSIMSKT SLLILKCRK*GWRREGEENQERFIEREDQKLLG TPYCRIKIFIMVFFLQNIPLYIIMFLHE*YKFK QEKYFLKSLRIFIKFRRPRISCVTWGMPLRL*AS ASLYVKWVEAALPHKHPVHRQCPAPLFSGLRGQ ACLGHTATYFWAVSSGNLLSCQCGQAL*TSMNQ ICFRLGLSTGEGGGKGHWGWECLLPSVSVTPVHP VPYLSLSGPTSFCCRLLPIE*QPSCFGFYFFVK SFPLQRTS*WV*KAAVATEAAERWPSAVPRLPT *VQLSPFLGLFKEVSRTDFRSLSMGPFSAPVEPG LL*TFRLASVEMTLPSTSTLGLKMEGTWCATRG RTEAGGPRRGRHTCLSRRGCPLTSASWCRAQIS R*W*TGSSSCSTSTACPSTVWTPSPSMALCSCPT SASSLPACGLPTRLPLPRQSSTQCRAPLDRCSL LPPSHL*CTPTPPIRCLSSPPFWEGCTHPSPSS CQALSCPVLRGVDLV*SSLPQGGRGWSAPV*ILP SPEEPAHHQQTGSGGRHPADPCADIGGFLALGP GAGVWGSLGPLIIPTSQAQPFQPCLGSGL*CRG HVLVWSCFWLQPPWNGEGS*RGLPSSAAAAPGAP AAGILPSQEAGTARERGGVGSEDEAPCSVPSHP PRSSTPVPSHQLSAPGGRWPPQPLLSDL*PHSH LAPCTNPSPLLESRPDGFPLASTT*PECSLQRTG SFPSVLKIKK*KCLLAHS

Sequence LGALS9.cAug05

TGTTTGTTAAATTATATGTAGGGACAGGGTCTTCCAACCTTTCCCGAGCTGGTCTCAAGCTCCTGGGCTGAAGTGATCCTCCTGCCTCAGCCTCTCAAAG TGCTGGTATTATAGGCATAAGCCACCACGCCCTGCCCATTTTTCTAAGTTGGATTATTTTACACTCCAGTCTGCTGTGAATGAGAGTTCCAGTTGTTGCG TATCGCAATCAATTTGTGATTGCCTACAAATGATGTGCCGTGATGAAATTGTCCCTGGTGTCCGAGAGGATCGCAAAGAATTTCCCTTCCTTTCTCTCCT TTCTGCTCCCCTTGTACTGTCTACTCCTTAAACTGGTACTTGATCCAGGAACATAATGGGGATGGACAGTGCAGATGGCACACATGGCCTCAGTCTATTG GAGCATCTCAAAGGAATTAGTCTGCAAATTCTAGTGCATCTAGGTGGAGCACAGGAAGGAAGAGAGGTGCAGACTCACGTATGGCCCTGGGGCACCTGCC ATCCTCTCGCACCCGTACAGCCCTTACCTGCCAGCACTTACCCTACCCAGCTTGAGTTCTGGGCCTTCTCACCAGCACATCGCTGGGACCTGGTCGACTT CAGGTAAGTTTATTTCATGGCTCCCTCCCCCACGACCTGCACATGCCATATGTTCCTTCCCAGCCCCATGTCACTTGAAATGTTCTATCATGTCCAAGAC TTCTCTCCTCATCCTAAAGTGCAGAAAATAAGGATGGAGAAGAGAAGGAGAAGAGAATCAAGAGAGATTCATTGAGAGGGAGGATCAGAAGCTCCTGGGC ACCCCATACTGTAGAATAAAAATATTCATTATGGTGTTTTTCTTGCAAAATATTCCCCTCTACATAATTATGTTTTTACATGAATAATATAAATTTAAAC AGGAGAAATACTTTTTGAAGAGTCTGAGAATCTTCATAAAGTTCAGGCGGCCACGAATTAGCTGTGTGACCTGGGGCATGCCCCTTAGACTCTGAGCCTC AGCTTCTCTGTATGTAAAATGGGTTGAAGCCGCCCTTCCCCACAAGCACCCTGTGCACAGGCAATGCCCAGCCCCATTATTTTCTGGACTCCGTGGCCAA GCATGCTTAGGACACACAGCCACATACTTCTGGGCAGTGTCATCTGGCAACTTGCTGTCATGTCAGTGTGGTCAAGCATTGTAGACCTCTATGAACCAAA TATGCTTCAGGCTTGGGTTGAGCACAGGAGAGGGAGGAGGGAAAGGTCACTGGGGCTGGGAGTGCCTACTTCCCTCTGTGAGTGTCACCCCAGTCCACCC AGTACCATACCTTTCTCTCTCTGGACCCACTTCCTTTTGCTGCCGGCTCCTCCCCATTGAATAACAGCCAAGTTGCTTTGGTTTCTATTTCTTTGTTAAG TCGTTCCCTCTACAAAGGACTTCCTAGTGGGTGTGAAAGGCAGCGGTGGCCACAGAGGCGGCGGAGAGATGGCCTTCAGCGGTTCCCAGGCTCCCTACCT GAGTCCAGCTGTCCCCTTTTCTGGGACTATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAGA TGGAGGGTACGTGGTGTGCAACACGAGGCAGAACGGAAGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGACCTC TGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGATCCTCTTCGTGCAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCT CCGTCAATGGCTCTGTGCAGCTGTCCTACATCAGCTTCCAGCCTCCCGGCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGT GCAGAGCGCCCCTGGACAGATGTTCTCTACTCCCGCCATCCCACCTATGATGTACCCCCACCCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGA GGGCTGTACCCATCCAAGTCCATCCTCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGCTCTGGGAACCACATCGCCTTCC ACCTGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGCAACACCCAGATCGACAACTCCTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCCTT CGTCCGTGGCCAGAGCTTCTCAGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTGGCCGTGGATGGTCAGCACCTGTTTGAATACTACCATCGCCTG AGGAACCTGCCCACCATCAACAGACTGGAAGTGGGGGGCGACATCCAGCTGACCCATGTGCAGACATAGGCGGCTTCCTGGCCCTGGGGCCGGGGGCTGG GGTGTGGGGCAGTCTGGGTCCTCTCATCATCCCCACTTCCCAGGCCCAGCCTTTCCAACCCTGCCTGGGATCTGGGCTTTAATGCAGAGGCCATGTCCTT GTCTGGTCCTGCTTCTGGCTACAGCCACCCTGGAACGGAGAAGGCAGCTGACGGGGATTGCCTTCCTCAGCCGCAGCAGCACCTGGGGCTCCAGCTGCTG GAATCCTACCATCCCAGGAGGCAGGCACAGCCAGGGAGAGGGGAGGAGTGGGCAGTGAAGATGAAGCCCCATGCTCAGTCCCCTCCCATCCCCCACGCAG CTCCACCCCAGTCCCAAGCCACCAGCTGTCTGCTCCTGGTGGGAGGTGGCCTCCTCAGCCCCTCCTCTCTGACCTTTAACCTCACTCTCACCTTGCACCG TGCACCAACCCTTCACCCCTCCTGGAAAGCAGGCCTGATGGCTTCCCACTGGCCTCCACCACCTGACCAGAGTGTTCTCTTCAGAGGACTGGCTCCTTTC CCAGTGTCCTTAAAATAAAGAAATGAAAATGCTTGTTGGCACATTCA

LGALS9.F10 (forward primer)
LGALS9.R6 (reverse primer)
Single exon skipping

Frame 0

CLLNYM*GQGLPTFPELVSSSWAEVILLPQPLK VLVL*A*ATTPCPFF*VGLFYTPVCCE*EFQLLR IAINL*LPTNDVP**NCPWCPRGSQRISLPFSP FCSPCTVYSLNWYLIQEHNGDGQCRWHTWPQSI GASQRN*SANSSASRWSTGRKRGADSRMALGHLP SSRTRTALTCQHLPYPA*VLGLLTSTSLGPGRL QVSLFHGSLPHDLHMPYVPSQPHVT*NVLSCPR LLSSS*SAENKDGEEKEKRIKRDSLRGRIRSSWA PHTVE*KYSLWCFSCKIFPST*LCFYMNNINLN RRNTF*RV*ESS*SSGGHELAV*PGACPLDSEP QLLCM*NGLKPPFPTSTLCTGNAQPHYFLDSVAK HA*DTQPHTSGQCHLATCCHVSVVKHCRPL*TK YASGLG*AQEREEGKVTGAGSAYFPL*VSPQST QYHTFLSLDPLPFAAGSSPLNNSQVALVSISLLS RSLYKGLPSGCERQRWPQRRRRDGLQRFPGSLP ESSCPLFWDYSRRSPGRTSDHCQWDRSQLQWNQ MEGTWCATRGRTEAGGPRRGRHTCLSRRGCPLTS ASWCRAQISR*W*TGSSSCSTSTACPSTVWTPS PSMALCSCPTSASSLPACGLPTRLPLPRQSSTQ CRAPLDRCSLLPPSHL*CTPTPPIRCLSSPPFWE GCTHPSPSSCQALSCPVLRGSTSTCALGTTSPS T*TPVLMRMLWSATPRSTTPGGLRSEVCPEKCP SSVARASQCGSCVKLTASRWPWMVSTCLNTTIA* GTCPPSTDWKWGATSS*PMCRHRRLPGPGAGGW GVGQSGSSHHPHFPGPAFPTLPGIWALMQRPCP CLVLLLATATLERRRQLTGIAFLSRSSTWGSSCW NPTIPGGRHSQGEGRSGQ*R*SPMLSPLPSPTQ LHPSPKPPAVCSWWEVASSAPPL*PLTSLSPCT VHQPFTPPGKQA*WLPTGLHHLTRVFSSEDWLLS QCP*NKEMKMLVGTF

Frame 1

VC*IICRDRVFQPFPSWSQAPGLK*SSCLSLSK CWYYRHKPPRPAHFSKLDYFTLQSAVNESSSCC VSQSICDCLQMMCRDEIVPGVREDRKEFPFLSLL SAPLVLSTP*TGT*SRNIMGMDSADGTHGLSLL EHLKGISLQILVHLGGAQEGREVQTHVWPWGTC HPLAPVQPLPASTYPTQLEFWAFSPAHRWDLVDF R*VYFMAPSPTTCTCHMFLPSPMSLEMFYHVQD FSPHPKVQKIRMEKRRRRESREIH*EGGSEAPG HPIL*NKNIHYGVFLAKYSPLHNYVFT*II*I*T GEILFEESENLHKVQAATN*LCDLGHAP*TLSL SFSVCKMG*SRPSPQAPCAQAMPSPIIFWTPWP SMLRTHSHILLGSVIWQLAVMSVWSSIVDLYEPN MLQAWVEHRRGRRERSLGLGVPTSLCECHPSPP STIPFSLWTHFLLLPAPPH*ITAKLLWFLFLC* VVPSTKDFLVGVKGSGGHRGGGEMAFSGSQAPYL SPAVPFSGTIQGGLQDGLQITVNGTVLSSSGTR WRVRGVQHEAERKLGARGEEDTHAFPEGDAL*P LLPGAELRFQGDGERDPLRAVLPPRALPPCGHHL RQWLCAAVLHQLPASRRVACQPGSHYPDSHPHS AERPWTDVLYSRHPTYDVPPPRLSDAFHHHHSG RAVPIQVHPPVRHCPAQCSEVPHQPVLWEPHRLP PEPPF**ECCGPQHPDRQLLGV*GAKSAPKNAL RPWPELLSVDLV*SSLPQGGRGWSAPV*ILPSP EEPAHHQQTGSGGRHPADPCADIGGFLALGPGAG VWGSLGPLIIPTSQAQPFQPCLGSGL*CRGHVL VWSCFWLQPPWNGEGS*RGLPSSAAAAPGAPAA GILPSQEAGTARERGGVGSEDEAPCSVPSHPPRS STPVPSHQLSAPGGRWPPQPLLSDL*PHSHLAP CTNPSPLLESRPDGFPLASTT*PECSLQRTGSF PSVLKIKK*KCLLAHS

Frame 2

FVKLYVGTGSSNLSRAGLKLLG*SDPPASASQS AGIIGISHHALPIFLSWIILHSSLL*MRVPVVA YRNQFVIAYK*CAVMKLSLVSERIAKNFPSFLS FLLPLYCLLLKLVLDPGT*WGWTVQMAHMASVYW SISKELVCKF*CI*VEHRKEERCRLTYGPGAPA ILSHPYSPYLPALTLPSLSSGPSHQHIAGTWST SGKFISWLPPPRPAHAICSFPAPCHLKCSIMSKT SLLILKCRK*GWRREGEENQERFIEREDQKLLG TPYCRIKIFIMVFFLQNIPLYIIMFLHE*YKFK QEKYFLKSLRIFIKFRRPRISCVTWGMPLRL*AS ASLYVKWVEAALPHKHPVHRQCPAPLFSGLRGQ ACLGHTATYFWAVSSGNLLSCQCGQAL*TSMNQ ICFRLGLSTGEGGGKGHWGWECLLPSVSVTPVHP VPYLSLSGPTSFCCRLLPIE*QPSCFGFYFFVK SFPLQRTS*WV*KAAVATEAAERWPSAVPRLPT *VQLSPFLGLFKEVSRTDFRSLSMGPFSAPVEPD GGYVVCNTRQNGSWGPEERKTHMPFQKGMPFDL CFLVQSSDFKVMVNGILFVQYFHRVPFHRVDTI SVNGSVQLSYISFQPPGVWPANPAPITQTVIHTV QSAPGQMFSTPAIPPMMYPHPAYPMPFITTILG GLYPSKSILLSGTVLPSAQRFHINLCSGNHIAF HLNPRFDENAVVRNTQIDNSWGSEERSLPRKMPF VRGQSFSVWILCEAHCLKVAVDGQHLFEYYHRL RNLPTINRLEVGGDIQLTHVQT*AASWPWGRGL GCGAVWVLSSSPLPRPSLSNPAWDLGFNAEAMSL SGPASGYSHPGTEKAADGDCLPQPQQHLGLQLL ESYHPRRQAQPGRGEEWAVKMKPHAQSPPIPHA APPQSQATSCLLLVGGGLLSPSSLTFNLTLTLHR APTLHPSWKAGLMASHWPPPPDQSVLFRGLAPF PVSLK*RNENACWHI

Sequence LGALS9.bAug05

TGTTTGTTAAATTATATGTAGGGACAGGGTCTTCCAACCTTTCCCGAGCTGGTCTCAAGCTCCTGGGCTGAAGTGATCCTCCTGCCTCAGCCTCTCAAAG TGCTGGTATTATAGGCATAAGCCACCACGCCCTGCCCATTTTTCTAAGTTGGATTATTTTACACTCCAGTCTGCTGTGAATGAGAGTTCCAGTTGTTGCG TATCGCAATCAATTTGTGATTGCCTACAAATGATGTGCCGTGATGAAATTGTCCCTGGTGTCCGAGAGGATCGCAAAGAATTTCCCTTCCTTTCTCTCCT TTCTGCTCCCCTTGTACTGTCTACTCCTTAAACTGGTACTTGATCCAGGAACATAATGGGGATGGACAGTGCAGATGGCACACATGGCCTCAGTCTATTG GAGCATCTCAAAGGAATTAGTCTGCAAATTCTAGTGCATCTAGGTGGAGCACAGGAAGGAAGAGAGGTGCAGACTCACGTATGGCCCTGGGGCACCTGCC ATCCTCTCGCACCCGTACAGCCCTTACCTGCCAGCACTTACCCTACCCAGCTTGAGTTCTGGGCCTTCTCACCAGCACATCGCTGGGACCTGGTCGACTT CAGGTAAGTTTATTTCATGGCTCCCTCCCCCACGACCTGCACATGCCATATGTTCCTTCCCAGCCCCATGTCACTTGAAATGTTCTATCATGTCCAAGAC TTCTCTCCTCATCCTAAAGTGCAGAAAATAAGGATGGAGAAGAGAAGGAGAAGAGAATCAAGAGAGATTCATTGAGAGGGAGGATCAGAAGCTCCTGGGC ACCCCATACTGTAGAATAAAAATATTCATTATGGTGTTTTTCTTGCAAAATATTCCCCTCTACATAATTATGTTTTTACATGAATAATATAAATTTAAAC AGGAGAAATACTTTTTGAAGAGTCTGAGAATCTTCATAAAGTTCAGGCGGCCACGAATTAGCTGTGTGACCTGGGGCATGCCCCTTAGACTCTGAGCCTC AGCTTCTCTGTATGTAAAATGGGTTGAAGCCGCCCTTCCCCACAAGCACCCTGTGCACAGGCAATGCCCAGCCCCATTATTTTCTGGACTCCGTGGCCAA GCATGCTTAGGACACACAGCCACATACTTCTGGGCAGTGTCATCTGGCAACTTGCTGTCATGTCAGTGTGGTCAAGCATTGTAGACCTCTATGAACCAAA TATGCTTCAGGCTTGGGTTGAGCACAGGAGAGGGAGGAGGGAAAGGTCACTGGGGCTGGGAGTGCCTACTTCCCTCTGTGAGTGTCACCCCAGTCCACCC AGTACCATACCTTTCTCTCTCTGGACCCACTTCCTTTTGCTGCCGGCTCCTCCCCATTGAATAACAGCCAAGTTGCTTTGGTTTCTATTTCTTTGTTAAG TCGTTCCCTCTACAAAGGACTTCCTAGTGGGTGTGAAAGGCAGCGGTGGCCACAGAGGCGGCGGAGAGATGGCCTTCAGCGGTTCCCAGGCTCCCTACCT GAGTCCAGCTGTCCCCTTTTCTGGGACTATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAGG TTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTCCACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGC AGAACGGAAGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGACCTCTGCTTCCTGGTGCAGAGCTCAGATTTCAA GGTGATGGTGAACGGGATCCTCTTCGTGCAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTGCAGCTGTCCTAC ATCAGCTTCCAGCCTCCCGGCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGTGCAGAGCGCCCCTGGACAGATGTTCTCTA CTCCCGCCATCCCACCTATGATGTACCCCCACCCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAGTCCATCCTCCT GTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGCTCTGGGAACCACATCGCCTTCCACCTGAACCCCCGTTTTGATGAGAATGCT GTGGTCCGCAACACCCAGATCGACAACTCCTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCCTTCGTCCGTGGCCAGAGCTTCTCAGTGTGGA TCTTGTGTGAAGCTCACTGCCTCAAGGTGGCCGTGGATGGTCAGCACCTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATCAACAGACTGGA AGTGGGGGGCGACATCCAGCTGACCCATGTGCAGACATAGGCGGCTTCCTGGCCCTGGGGCCGGGGGCTGGGGTGTGGGGCAGTCTGGGTCCTCTCATCA TCCCCACTTCCCAGGCCCAGCCTTTCCAACCCTGCCTGGGATCTGGGCTTTAATGCAGAGGCCATGTCCTTGTCTGGTCCTGCTTCTGGCTACAGCCACC CTGGAACGGAGAAGGCAGCTGACGGGGATTGCCTTCCTCAGCCGCAGCAGCACCTGGGGCTCCAGCTGCTGGAATCCTACCATCCCAGGAGGCAGGCACA GCCAGGGAGAGGGGAGGAGTGGGCAGTGAAGATGAAGCCCCATGCTCAGTCCCCTCCCATCCCCCACGCAGCTCCACCCCAGTCCCAAGCCACCAGCTGT CTGCTCCTGGTGGGAGGTGGCCTCCTCAGCCCCTCCTCTCTGACCTTTAACCTCACTCTCACCTTGCACCGTGCACCAACCCTTCACCCCTCCTGGAAAG CAGGCCTGATGGCTTCCCACTGGCCTCCACCACCTGACCAGAGTGTTCTCTTCAGAGGACTGGCTCCTTTCCCAGTGTCCTTAAAATAAAGAAATGAAAA TGCTTGTTGGCACATTCA

LGALS9.F10 (forward primer)
LGALS9.R6 (reverse primer)
Single exon skipping

Frame 0

CLLNYM*GQGLPTFPELVSSSWAEVILLPQPLK VLVL*A*ATTPCPFF*VGLFYTPVCCE*EFQLLR IAINL*LPTNDVP**NCPWCPRGSQRISLPFSP FCSPCTVYSLNWYLIQEHNGDGQCRWHTWPQSI GASQRN*SANSSASRWSTGRKRGADSRMALGHLP SSRTRTALTCQHLPYPA*VLGLLTSTSLGPGRL QVSLFHGSLPHDLHMPYVPSQPHVT*NVLSCPR LLSSS*SAENKDGEEKEKRIKRDSLRGRIRSSWA PHTVE*KYSLWCFSCKIFPST*LCFYMNNINLN RRNTF*RV*ESS*SSGGHELAV*PGACPLDSEP QLLCM*NGLKPPFPTSTLCTGNAQPHYFLDSVAK HA*DTQPHTSGQCHLATCCHVSVVKHCRPL*TK YASGLG*AQEREEGKVTGAGSAYFPL*VSPQST QYHTFLSLDPLPFAAGSSPLNNSQVALVSISLLS RSLYKGLPSGCERQRWPQRRRRDGLQRFPGSLP ESSCPLFWDYSRRSPGRTSDHCQWDRSQLQWNQ VCCELSDWLQWK*HCLPLQPSV*RWRVRGVQHEA ERKLGARGEEDTHAFPEGDAL*PLLPGAELRFQ GDGERDPLRAVLPPRALPPCGHHLRQWLCAAVL HQLPASRRVACQPGSHYPDSHPHSAERPWTDVLY SRHPTYDVPPPRLSDAFHHHHSGRAVPIQVHPP VRHCPAQCSEVPHQPVLWEPHRLPPEPPF**EC CGPQHPDRQLLGV*GAKSAPKNALRPWPELLSVD LV*SSLPQGGRGWSAPV*ILPSPEEPAHHQQTG SGGRHPADPCADIGGFLALGPGAGVWGSLGPLI IPTSQAQPFQPCLGSGL*CRGHVLVWSCFWLQPP WNGEGS*RGLPSSAAAAPGAPAAGILPSQEAGT ARERGGVGSEDEAPCSVPSHPPRSSTPVPSHQL SAPGGRWPPQPLLSDL*PHSHLAPCTNPSPLLES RPDGFPLASTT*PECSLQRTGSFPSVLKIKK*K CLLAHS

Frame 1

VC*IICRDRVFQPFPSWSQAPGLK*SSCLSLSK CWYYRHKPPRPAHFSKLDYFTLQSAVNESSSCC VSQSICDCLQMMCRDEIVPGVREDRKEFPFLSLL SAPLVLSTP*TGT*SRNIMGMDSADGTHGLSLL EHLKGISLQILVHLGGAQEGREVQTHVWPWGTC HPLAPVQPLPASTYPTQLEFWAFSPAHRWDLVDF R*VYFMAPSPTTCTCHMFLPSPMSLEMFYHVQD FSPHPKVQKIRMEKRRRRESREIH*EGGSEAPG HPIL*NKNIHYGVFLAKYSPLHNYVFT*II*I*T GEILFEESENLHKVQAATN*LCDLGHAP*TLSL SFSVCKMG*SRPSPQAPCAQAMPSPIIFWTPWP SMLRTHSHILLGSVIWQLAVMSVWSSIVDLYEPN MLQAWVEHRRGRRERSLGLGVPTSLCECHPSPP STIPFSLWTHFLLLPAPPH*ITAKLLWFLFLC* VVPSTKDFLVGVKGSGGHRGGGEMAFSGSQAPYL SPAVPFSGTIQGGLQDGLQITVNGTVLSSSGTR FAVNFQTGFSGNDIAFHFNPRFEDGGYVVCNTR QNGSWGPEERKTHMPFQKGMPFDLCFLVQSSDFK VMVNGILFVQYFHRVPFHRVDTISVNGSVQLSY ISFQPPGVWPANPAPITQTVIHTVQSAPGQMFS TPAIPPMMYPHPAYPMPFITTILGGLYPSKSILL SGTVLPSAQRFHINLCSGNHIAFHLNPRFDENA VVRNTQIDNSWGSEERSLPRKMPFVRGQSFSVW ILCEAHCLKVAVDGQHLFEYYHRLRNLPTINRLE VGGDIQLTHVQT*AASWPWGRGLGCGAVWVLSS SPLPRPSLSNPAWDLGFNAEAMSLSGPASGYSH PGTEKAADGDCLPQPQQHLGLQLLESYHPRRQAQ PGRGEEWAVKMKPHAQSPPIPHAAPPQSQATSC LLLVGGGLLSPSSLTFNLTLTLHRAPTLHPSWK AGLMASHWPPPPDQSVLFRGLAPFPVSLK*RNEN ACWHI

Frame 2

FVKLYVGTGSSNLSRAGLKLLG*SDPPASASQS AGIIGISHHALPIFLSWIILHSSLL*MRVPVVA YRNQFVIAYK*CAVMKLSLVSERIAKNFPSFLS FLLPLYCLLLKLVLDPGT*WGWTVQMAHMASVYW SISKELVCKF*CI*VEHRKEERCRLTYGPGAPA ILSHPYSPYLPALTLPSLSSGPSHQHIAGTWST SGKFISWLPPPRPAHAICSFPAPCHLKCSIMSKT SLLILKCRK*GWRREGEENQERFIEREDQKLLG TPYCRIKIFIMVFFLQNIPLYIIMFLHE*YKFK QEKYFLKSLRIFIKFRRPRISCVTWGMPLRL*AS ASLYVKWVEAALPHKHPVHRQCPAPLFSGLRGQ ACLGHTATYFWAVSSGNLLSCQCGQAL*TSMNQ ICFRLGLSTGEGGGKGHWGWECLLPSVSVTPVHP VPYLSLSGPTSFCCRLLPIE*QPSCFGFYFFVK SFPLQRTS*WV*KAAVATEAAERWPSAVPRLPT *VQLSPFLGLFKEVSRTDFRSLSMGPFSAPVEPG LL*TFRLASVEMTLPSTSTLGLKMEGTWCATRG RTEAGGPRRGRHTCLSRRGCPLTSASWCRAQIS R*W*TGSSSCSTSTACPSTVWTPSPSMALCSCPT SASSLPACGLPTRLPLPRQSSTQCRAPLDRCSL LPPSHL*CTPTPPIRCLSSPPFWEGCTHPSPSS CQALSCPVLRGSTSTCALGTTSPST*TPVLMRML WSATPRSTTPGGLRSEVCPEKCPSSVARASQCG SCVKLTASRWPWMVSTCLNTTIA*GTCPPSTDW KWGATSS*PMCRHRRLPGPGAGGWGVGQSGSSHH PHFPGPAFPTLPGIWALMQRPCPCLVLLLATAT LERRRQLTGIAFLSRSSTWGSSCWNPTIPGGRH SQGEGRSGQ*R*SPMLSPLPSPTQLHPSPKPPAV CSWWEVASSAPPL*PLTSLSPCTVHQPFTPPGK QA*WLPTGLHHLTRVFSSEDWLLSQCP*NKEMK MLVGTF

Sequence LGALS9.iAug05

TGTTTGTTAAATTATATGTAGGGACAGGGTCTTCCAACCTTTCCCGAGCTGGTCTCAAGCTCCTGGGCTGAAGTGATCCTCCTGCCTCAGCCTCTCAAAG TGCTGGTATTATAGGCATAAGCCACCACGCCCTGCCCATTTTTCTAAGTTGGATTATTTTACACTCCAGTCTGCTGTGAATGAGAGTTCCAGTTGTTGCG TATCGCAATCAATTTGTGATTGCCTACAAATGATGTGCCGTGATGAAATTGTCCCTGGTGTCCGAGAGGATCGCAAAGAATTTCCCTTCCTTTCTCTCCT TTCTGCTCCCCTTGTACTGTCTACTCCTTAAACTGGTACTTGATCCAGGAACATAATGGGGATGGACAGTGCAGATGGCACACATGGCCTCAGTCTATTG GAGCATCTCAAAGGAATTAGTCTGCAAATTCTAGTGCATCTAGGTGGAGCACAGGAAGGAAGAGAGGTGCAGACTCACGTATGGCCCTGGGGCACCTGCC ATCCTCTCGCACCCGTACAGCCCTTACCTGCCAGCACTTACCCTACCCAGCTTGAGTTCTGGGCCTTCTCACCAGCACATCGCTGGGACCTGGTCGACTT CAGGTAAGTTTATTTCATGGCTCCCTCCCCCACGACCTGCACATGCCATATGTTCCTTCCCAGCCCCATGTCACTTGAAATGTTCTATCATGTCCAAGAC TTCTCTCCTCATCCTAAAGTGCAGAAAATAAGGATGGAGAAGAGAAGGAGAAGAGAATCAAGAGAGATTCATTGAGAGGGAGGATCAGAAGCTCCTGGGC ACCCCATACTGTAGAATAAAAATATTCATTATGGTGTTTTTCTTGCAAAATATTCCCCTCTACATAATTATGTTTTTACATGAATAATATAAATTTAAAC AGGAGAAATACTTTTTGAAGAGTCTGAGAATCTTCATAAAGTTCAGGCGGCCACGAATTAGCTGTGTGACCTGGGGCATGCCCCTTAGACTCTGAGCCTC AGCTTCTCTGTATGTAAAATGGGTTGAAGCCGCCCTTCCCCACAAGCACCCTGTGCACAGGCAATGCCCAGCCCCATTATTTTCTGGACTCCGTGGCCAA GCATGCTTAGGACACACAGCCACATACTTCTGGGCAGTGTCATCTGGCAACTTGCTGTCATGTCAGTGTGGTCAAGCATTGTAGACCTCTATGAACCAAA TATGCTTCAGGCTTGGGTTGAGCACAGGAGAGGGAGGAGGGAAAGGTCACTGGGGCTGGGAGTGCCTACTTCCCTCTGTGAGTGTCACCCCAGTCCACCC AGTACCATACCTTTCTCTCTCTGGACCCACTTCCTTTTGCTGCCGGCTCCTCCCCATTGAATAACAGCCAAGTTGCTTTGGTTTCTATTTCTTTGTTAAG TCGTTCCCTCTACAAAGGACTTCCTAGTGGGTGTGAAAGGCAGCGGTGGCCACAGAGGCGGCGGAGAGATGGCCTTCAGCGGTTCCCAGGCTCCCTACCT GAGTCCAGCTGTCCCCTTTTCTGGGACTATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAGA TGGAGGGTACGTGGTGTGCAACACGAGGCAGAACGGAAGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGACCTC TGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGATCCTCTTCGTGCAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCT CCGTCAATGGCTCTGTGCAGCTGTCCTACATCAGCTTCCAGAACCCCCGCACAGTCCCTGTTCAGCCTGCCTTCTCCACGGTGCCGTTCTCCCAGCCTGT CTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAACCTCCCGGCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGTGCAG AGCGCCCCTGGACAGATGTTCTCTACTCCCGCCATCCCACCTATGATGTACCCCCACCCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGC TGTACCCATCCAAGTCCATCCTCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTAAGCCAAGGGCTCCAGTGACCTCTGGGAAGAGAGAGCCCTTCA AGGTCAGTCCAGCCATTCCCCTGGCTTCAGGAAGGCTACTGATGATGGGGAGGAAATGGGACTCAGAATTCGGTGGATAAAGGTTCAGGTGGGCTGCCCA CCCCAGGTTCCACATCAACCTGTGCTCTGGGAACCACATCGCCTTCCACCTGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGCAACACCCAGATCGAC AACTCCTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCCTTCGTCCGTGGCCAGAGCTTCTCAGTAAGGCACCGCAGTCTGGAGCTTGGAGAGG CTCCCATGGGTGCACAGGGGGAGGGGGTAAAGGAGGTTTGAGGTACCTTGAACAGTATGGGGGCTGATTCCTCTGGGATGAAGGCCCAAAAGAAGAGAAG GTGGCCAACAAATTATTATTATTATTACTGTCCCGCATGAAGGAAGTGCTGGGATCTGCTTTTTCAGATGAGGAAACAAACTCACAAGTGCAGGTTACTT GCTTGTGGTCACACAGTCAGTAAGTGTCAAATCAGACCCAAGAAGGAGGTGTTATTCATTTCTTCATTTACTCAACAACCATTTTCTTGTCGCCTGCTTT GCGCAGGAAGCACCCCACATGCTGGGGGCACAGTCATGAAGCACACAGACAGGGTCCTGCCTCCAGGGAGTTTATAATTTAGGTGGGGAAAGAACAAGGA AAGTAAAGCATGGACAGATTGTCACAGATAACGACAGTGCCACGCACAAAGCAAAACAGAGCGATGAGATAGAGCGAGCCTGGGAGGGGCAGCTAGGGCC AACGTGCAGAGGCACGGGTGTGGCCTGTCCAGGAACAGCAGGCAGGCGTGCTCCACCCTGACTACATATTCAAATCACCCAGAGAGCTTACAAAGGAAAT ATCAGTGCTGGGACCCCAGCCCAGGCCAATGAGGCAGTCAGCTGGGCATTCGTATTTTAAAAATATGCCTTAAGTGATTATACTCTGCAGTCAGAGTTGA AAATGACTGATGTAGGGCTTTGCAGCCATGGAAAGGAGCTTGGATTTTAACACAGTGAAAAGCCTCAGGAGTGTTTCAAGCCAGGGAGCAACATACTTTG ATTTGTAGACAGCTGTGTGACCTCGGACAAGTGACTTAATTATTCTGTGCTTCAGTTTCCTCATCTACAAAATGGAGCTAATAATCATGAATTTCCTAGA GTTGTAAGAATGGAATGAGACAGTATCTGTAGTATGCTTAGGACAGTGCCTGGCAGGTGGCATGTGTGATAGGAGTGTTAGCTATTGCAAAGGGAAGGAG GTTTTAACATTTGTACCCTGACTGCTTCGTGGAGAAGGCATTGGGGTGGAAAAGACAAGTTAGGAGACTGATGCTGCAGGAGCCCAGGCCCAGAGCACTC CTGTGTGCTGGGCCTGAGGGATACAGACGGCACAGGCCAGTGCTCCTGTCCCTGATGGGGTGGGAACAGTATTCTAGACCAGGGAACAGTACAGGGGAAA GAGCAC

LGALS9.F10 (forward primer)
LGALS9.R6 (reverse primer)
Single exon skipping

Frame 0

CLLNYM*GQGLPTFPELVSSSWAEVILLPQPLK VLVL*A*ATTPCPFF*VGLFYTPVCCE*EFQLLR IAINL*LPTNDVP**NCPWCPRGSQRISLPFSP FCSPCTVYSLNWYLIQEHNGDGQCRWHTWPQSI GASQRN*SANSSASRWSTGRKRGADSRMALGHLP SSRTRTALTCQHLPYPA*VLGLLTSTSLGPGRL QVSLFHGSLPHDLHMPYVPSQPHVT*NVLSCPR LLSSS*SAENKDGEEKEKRIKRDSLRGRIRSSWA PHTVE*KYSLWCFSCKIFPST*LCFYMNNINLN RRNTF*RV*ESS*SSGGHELAV*PGACPLDSEP QLLCM*NGLKPPFPTSTLCTGNAQPHYFLDSVAK HA*DTQPHTSGQCHLATCCHVSVVKHCRPL*TK YASGLG*AQEREEGKVTGAGSAYFPL*VSPQST QYHTFLSLDPLPFAAGSSPLNNSQVALVSISLLS RSLYKGLPSGCERQRWPQRRRRDGLQRFPGSLP ESSCPLFWDYSRRSPGRTSDHCQWDRSQLQWNQ MEGTWCATRGRTEAGGPRRGRHTCLSRRGCPLTS ASWCRAQISR*W*TGSSSCSTSTACPSTVWTPS PSMALCSCPTSASRTPAQSLFSLPSPRCRSPSL SVSHPGPGGADKNLPACGLPTRLPLPRQSSTQCR APLDRCSLLPPSHL*CTPTPPIRCLSSPPFWEG CTHPSPSSCQALSCPVLRGKPRAPVTSGKREPF KVSPAIPLASGRLLMMGRKWDSEFGG*RFRWAAH PRFHINLCSGNHIAFHLNPRFDENAVVRNTQID NSWGSEERSLPRKMPFVRGQSFSVRHRSLELGE APMGAQGEGVKEV*GTLNSMGADSSGMKAQKKRR WPTNYYYYYCPA*RKCWDLLFQMRKQTHKCRLL ACGHTVSKCQIRPKKEVLFISSFTQQPFSCRLL CAGSTPHAGGTVMKHTDRVLPPGSL*FRWGKNKE SKAWTDCHR*RQCHAQSKTER*DRASLGGAARA NVQRHGCGLSRNSRQACSTLTTYSNHPESLQRK YQCWDPSPGQ*GSQLGIRILKICLK*LYSAVRVE ND*CRALQPWKGAWILTQ*KASGVFQAREQHTL ICRQLCDLGQVT*LFCASVSSSTKWS**S*IS* SCKNGMRQYL*YA*DSAWQVACVIGVLAIAKGRR F*HLYPDCFVEKALGWKRQVRRLMLQEPRPRAL LCAGPEGYRRHRPVLLSLMGWEQYSRPGNSTGE RA

Frame 1

VC*IICRDRVFQPFPSWSQAPGLK*SSCLSLSK CWYYRHKPPRPAHFSKLDYFTLQSAVNESSSCC VSQSICDCLQMMCRDEIVPGVREDRKEFPFLSLL SAPLVLSTP*TGT*SRNIMGMDSADGTHGLSLL EHLKGISLQILVHLGGAQEGREVQTHVWPWGTC HPLAPVQPLPASTYPTQLEFWAFSPAHRWDLVDF R*VYFMAPSPTTCTCHMFLPSPMSLEMFYHVQD FSPHPKVQKIRMEKRRRRESREIH*EGGSEAPG HPIL*NKNIHYGVFLAKYSPLHNYVFT*II*I*T GEILFEESENLHKVQAATN*LCDLGHAP*TLSL SFSVCKMG*SRPSPQAPCAQAMPSPIIFWTPWP SMLRTHSHILLGSVIWQLAVMSVWSSIVDLYEPN MLQAWVEHRRGRRERSLGLGVPTSLCECHPSPP STIPFSLWTHFLLLPAPPH*ITAKLLWFLFLC* VVPSTKDFLVGVKGSGGHRGGGEMAFSGSQAPYL SPAVPFSGTIQGGLQDGLQITVNGTVLSSSGTR WRVRGVQHEAERKLGARGEEDTHAFPEGDAL*P LLPGAELRFQGDGERDPLRAVLPPRALPPCGHHL RQWLCAAVLHQLPEPPHSPCSACLLHGAVLPAC LFPTQAQGAQTKTSRRVACQPGSHYPDSHPHSA ERPWTDVLYSRHPTYDVPPPRLSDAFHHHHSGRA VPIQVHPPVRHCPAQCSEVSQGLQ*PLGRESPS RSVQPFPWLQEGY**WGGNGTQNSVDKGSGGLP TPGSTSTCALGTTSPST*TPVLMRMLWSATPRST TPGGLRSEVCPEKCPSSVARASQ*GTAVWSLER LPWVHRGRG*RRFEVP*TVWGLIPLG*RPKRRE GGQQIIIIITVPHEGSAGICFFR*GNKLTSAGYL LVVTQSVSVKSDPRRRCYSFLHLLNNHFLVACF AQEAPHMLGAQS*STQTGSCLQGVYNLGGERTR KVKHGQIVTDNDSATHKAKQSDEIERAWEGQLGP TCRGTGVACPGTAGRRAPP*LHIQITQRAYKGN ISAGTPAQANEAVSWAFVF*KYALSDYTLQSEL KMTDVGLCSHGKELGF*HSEKPQECFKPGSNIL* FVDSCVTSDK*LNYSVLQFPHLQNGANNHEFPR VVRME*DSICSMLRTVPGRWHV**EC*LLQREG GFNICTLTASWRRHWGGKDKLGD*CCRSPGPEHS CVLGLRDTDGTGQCSCP*WGGNSILDQGTVQGK EH

Frame 2

FVKLYVGTGSSNLSRAGLKLLG*SDPPASASQS AGIIGISHHALPIFLSWIILHSSLL*MRVPVVA YRNQFVIAYK*CAVMKLSLVSERIAKNFPSFLS FLLPLYCLLLKLVLDPGT*WGWTVQMAHMASVYW SISKELVCKF*CI*VEHRKEERCRLTYGPGAPA ILSHPYSPYLPALTLPSLSSGPSHQHIAGTWST SGKFISWLPPPRPAHAICSFPAPCHLKCSIMSKT SLLILKCRK*GWRREGEENQERFIEREDQKLLG TPYCRIKIFIMVFFLQNIPLYIIMFLHE*YKFK QEKYFLKSLRIFIKFRRPRISCVTWGMPLRL*AS ASLYVKWVEAALPHKHPVHRQCPAPLFSGLRGQ ACLGHTATYFWAVSSGNLLSCQCGQAL*TSMNQ ICFRLGLSTGEGGGKGHWGWECLLPSVSVTPVHP VPYLSLSGPTSFCCRLLPIE*QPSCFGFYFFVK SFPLQRTS*WV*KAAVATEAAERWPSAVPRLPT *VQLSPFLGLFKEVSRTDFRSLSMGPFSAPVEPD GGYVVCNTRQNGSWGPEERKTHMPFQKGMPFDL CFLVQSSDFKVMVNGILFVQYFHRVPFHRVDTI SVNGSVQLSYISFQNPRTVPVQPAFSTVPFSQPV CFPPRPRGRRQKPPGVWPANPAPITQTVIHTVQ SAPGQMFSTPAIPPMMYPHPAYPMPFITTILGG LYPSKSILLSGTVLPSAQR*AKGSSDLWEERALQ GQSSHSPGFRKATDDGEEMGLRIRWIKVQVGCP PQVPHQPVLWEPHRLPPEPPF**ECCGPQHPDR QLLGV*GAKSAPKNALRPWPELLSKAPQSGAWRG SHGCTGGGGKGGLRYLEQYGG*FLWDEGPKEEK VANKLLLLLLSRMKEVLGSAFSDEETNSQVQVT CLWSHSQ*VSNQTQEGGVIHFFIYSTTIFLSPAL RRKHPTCWGHSHEAHRQGPASREFII*VGKEQG K*SMDRLSQITTVPRTKQNRAMR*SEPGRGS*G QRAEARVWPVQEQQAGVLHPDYIFKSPRELTKEI SVLGPQPRPMRQSAGHSYFKNMP*VIILCSQS* K*LM*GFAAMERSLDFNTVKSLRSVSSQGATYF DL*TAV*PRTSDLIILCFSFLIYKMELIIMNFLE L*EWNETVSVVCLGQCLAGGMCDRSVSYCKGKE VLTFVP*LLRGEGIGVEKTS*ETDAAGAQAQST PVCWA*GIQTAQASAPVPDGVGTVF*TREQYRGK S

Sequence LGALS9.aAug05

TGTTTGTTAAATTATATGTAGGGACAGGGTCTTCCAACCTTTCCCGAGCTGGTCTCAAGCTCCTGGGCTGAAGTGATCCTCCTGCCTCAGCCTCTCAAAG TGCTGGTATTATAGGCATAAGCCACCACGCCCTGCCCATTTTTCTAAGTTGGATTATTTTACACTCCAGTCTGCTGTGAATGAGAGTTCCAGTTGTTGCG TATCGCAATCAATTTGTGATTGCCTACAAATGATGTGCCGTGATGAAATTGTCCCTGGTGTCCGAGAGGATCGCAAAGAATTTCCCTTCCTTTCTCTCCT TTCTGCTCCCCTTGTACTGTCTACTCCTTAAACTGGTACTTGATCCAGGAACATAATGGGGATGGACAGTGCAGATGGCACACATGGCCTCAGTCTATTG GAGCATCTCAAAGGAATTAGTCTGCAAATTCTAGTGCATCTAGGTGGAGCACAGGAAGGAAGAGAGGTGCAGACTCACGTATGGCCCTGGGGCACCTGCC ATCCTCTCGCACCCGTACAGCCCTTACCTGCCAGCACTTACCCTACCCAGCTTGAGTTCTGGGCCTTCTCACCAGCACATCGCTGGGACCTGGTCGACTT CAGGTAAGTTTATTTCATGGCTCCCTCCCCCACGACCTGCACATGCCATATGTTCCTTCCCAGCCCCATGTCACTTGAAATGTTCTATCATGTCCAAGAC TTCTCTCCTCATCCTAAAGTGCAGAAAATAAGGATGGAGAAGAGAAGGAGAAGAGAATCAAGAGAGATTCATTGAGAGGGAGGATCAGAAGCTCCTGGGC ACCCCATACTGTAGAATAAAAATATTCATTATGGTGTTTTTCTTGCAAAATATTCCCCTCTACATAATTATGTTTTTACATGAATAATATAAATTTAAAC AGGAGAAATACTTTTTGAAGAGTCTGAGAATCTTCATAAAGTTCAGGCGGCCACGAATTAGCTGTGTGACCTGGGGCATGCCCCTTAGACTCTGAGCCTC AGCTTCTCTGTATGTAAAATGGGTTGAAGCCGCCCTTCCCCACAAGCACCCTGTGCACAGGCAATGCCCAGCCCCATTATTTTCTGGACTCCGTGGCCAA GCATGCTTAGGACACACAGCCACATACTTCTGGGCAGTGTCATCTGGCAACTTGCTGTCATGTCAGTGTGGTCAAGCATTGTAGACCTCTATGAACCAAA TATGCTTCAGGCTTGGGTTGAGCACAGGAGAGGGAGGAGGGAAAGGTCACTGGGGCTGGGAGTGCCTACTTCCCTCTGTGAGTGTCACCCCAGTCCACCC AGTACCATACCTTTCTCTCTCTGGACCCACTTCCTTTTGCTGCCGGCTCCTCCCCATTGAATAACAGCCAAGTTGCTTTGGTTTCTATTTCTTTGTTAAG TCGTTCCCTCTACAAAGGACTTCCTAGTGGGTGTGAAAGGCAGCGGTGGCCACAGAGGCGGCGGAGAGATGGCCTTCAGCGGTTCCCAGGCTCCCTACCT GAGTCCAGCTGTCCCCTTTTCTGGGACTATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAGG TTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTCCACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGC AGAACGGAAGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGACCTCTGCTTCCTGGTGCAGAGCTCAGATTTCAA GGTGATGGTGAACGGGATCCTCTTCGTGCAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTGCAGCTGTCCTAC ATCAGCTTCCAGAACCCCCGCACAGTCCCTGTTCAGCCTGCCTTCTCCACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCA GACAAAAACCTCCCGGCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGTGCAGAGCGCCCCTGGACAGATGTTCTCTACTCC CGCCATCCCACCTATGATGTACCCCCACCCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAGTCCATCCTCCTGTCA GGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGCTCTGGGAACCACATCGCCTTCCACCTGAACCCCCGTTTTGATGAGAATGCTGTGG TCCGCAACACCCAGATCGACAACTCCTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCCTTCGTCCGTGGCCAGAGCTTCTCAGTGTGGATCTT GTGTGAAGCTCACTGCCTCAAGGTGGCCGTGGATGGTCAGCACCTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATCAACAGACTGGAAGTG GGGGGCGACATCCAGCTGACCCATGTGCAGACATAGGCGGCTTCCTGGCCCTGGGGCCGGGGGCTGGGGTGTGGGGCAGTCTGGGTCCTCTCATCATCCC CACTTCCCAGGCCCAGCCTTTCCAACCCTGCCTGGGATCTGGGCTTTAATGCAGAGGCCATGTCCTTGTCTGGTCCTGCTTCTGGCTACAGCCACCCTGG AACGGAGAAGGCAGCTGACGGGGATTGCCTTCCTCAGCCGCAGCAGCACCTGGGGCTCCAGCTGCTGGAATCCTACCATCCCAGGAGGCAGGCACAGCCA GGGAGAGGGGAGGAGTGGGCAGTGAAGATGAAGCCCCATGCTCAGTCCCCTCCCATCCCCCACGCAGCTCCACCCCAGTCCCAAGCCACCAGCTGTCTGC TCCTGGTGGGAGGTGGCCTCCTCAGCCCCTCCTCTCTGACCTTTAACCTCACTCTCACCTTGCACCGTGCACCAACCCTTCACCCCTCCTGGAAAGCAGG CCTGATGGCTTCCCACTGGCCTCCACCACCTGACCAGAGTGTTCTCTTCAGAGGACTGGCTCCTTTCCCAGTGTCCTTAAAATAAAGAAATGAAAATGCT TGTTGGCACATTCA

LGALS9.F10 (forward primer)
LGALS9.R6 (reverse primer)
Single exon skipping

Frame 0

CLLNYM*GQGLPTFPELVSSSWAEVILLPQPLK VLVL*A*ATTPCPFF*VGLFYTPVCCE*EFQLLR IAINL*LPTNDVP**NCPWCPRGSQRISLPFSP FCSPCTVYSLNWYLIQEHNGDGQCRWHTWPQSI GASQRN*SANSSASRWSTGRKRGADSRMALGHLP SSRTRTALTCQHLPYPA*VLGLLTSTSLGPGRL QVSLFHGSLPHDLHMPYVPSQPHVT*NVLSCPR LLSSS*SAENKDGEEKEKRIKRDSLRGRIRSSWA PHTVE*KYSLWCFSCKIFPST*LCFYMNNINLN RRNTF*RV*ESS*SSGGHELAV*PGACPLDSEP QLLCM*NGLKPPFPTSTLCTGNAQPHYFLDSVAK HA*DTQPHTSGQCHLATCCHVSVVKHCRPL*TK YASGLG*AQEREEGKVTGAGSAYFPL*VSPQST QYHTFLSLDPLPFAAGSSPLNNSQVALVSISLLS RSLYKGLPSGCERQRWPQRRRRDGLQRFPGSLP ESSCPLFWDYSRRSPGRTSDHCQWDRSQLQWNQ VCCELSDWLQWK*HCLPLQPSV*RWRVRGVQHEA ERKLGARGEEDTHAFPEGDAL*PLLPGAELRFQ GDGERDPLRAVLPPRALPPCGHHLRQWLCAAVL HQLPEPPHSPCSACLLHGAVLPACLFPTQAQGAQ TKTSRRVACQPGSHYPDSHPHSAERPWTDVLYS RHPTYDVPPPRLSDAFHHHHSGRAVPIQVHPPV RHCPAQCSEVPHQPVLWEPHRLPPEPPF**ECCG PQHPDRQLLGV*GAKSAPKNALRPWPELLSVDL V*SSLPQGGRGWSAPV*ILPSPEEPAHHQQTGS GGRHPADPCADIGGFLALGPGAGVWGSLGPLIIP TSQAQPFQPCLGSGL*CRGHVLVWSCFWLQPPW NGEGS*RGLPSSAAAAPGAPAAGILPSQEAGTA RERGGVGSEDEAPCSVPSHPPRSSTPVPSHQLSA PGGRWPPQPLLSDL*PHSHLAPCTNPSPLLESR PDGFPLASTT*PECSLQRTGSFPSVLKIKK*KC LLAHS

Frame 1

VC*IICRDRVFQPFPSWSQAPGLK*SSCLSLSK CWYYRHKPPRPAHFSKLDYFTLQSAVNESSSCC VSQSICDCLQMMCRDEIVPGVREDRKEFPFLSLL SAPLVLSTP*TGT*SRNIMGMDSADGTHGLSLL EHLKGISLQILVHLGGAQEGREVQTHVWPWGTC HPLAPVQPLPASTYPTQLEFWAFSPAHRWDLVDF R*VYFMAPSPTTCTCHMFLPSPMSLEMFYHVQD FSPHPKVQKIRMEKRRRRESREIH*EGGSEAPG HPIL*NKNIHYGVFLAKYSPLHNYVFT*II*I*T GEILFEESENLHKVQAATN*LCDLGHAP*TLSL SFSVCKMG*SRPSPQAPCAQAMPSPIIFWTPWP SMLRTHSHILLGSVIWQLAVMSVWSSIVDLYEPN MLQAWVEHRRGRRERSLGLGVPTSLCECHPSPP STIPFSLWTHFLLLPAPPH*ITAKLLWFLFLC* VVPSTKDFLVGVKGSGGHRGGGEMAFSGSQAPYL SPAVPFSGTIQGGLQDGLQITVNGTVLSSSGTR FAVNFQTGFSGNDIAFHFNPRFEDGGYVVCNTR QNGSWGPEERKTHMPFQKGMPFDLCFLVQSSDFK VMVNGILFVQYFHRVPFHRVDTISVNGSVQLSY ISFQNPRTVPVQPAFSTVPFSQPVCFPPRPRGR RQKPPGVWPANPAPITQTVIHTVQSAPGQMFSTP AIPPMMYPHPAYPMPFITTILGGLYPSKSILLS GTVLPSAQRFHINLCSGNHIAFHLNPRFDENAV VRNTQIDNSWGSEERSLPRKMPFVRGQSFSVWIL CEAHCLKVAVDGQHLFEYYHRLRNLPTINRLEV GGDIQLTHVQT*AASWPWGRGLGCGAVWVLSSS PLPRPSLSNPAWDLGFNAEAMSLSGPASGYSHPG TEKAADGDCLPQPQQHLGLQLLESYHPRRQAQP GRGEEWAVKMKPHAQSPPIPHAAPPQSQATSCL LLVGGGLLSPSSLTFNLTLTLHRAPTLHPSWKAG LMASHWPPPPDQSVLFRGLAPFPVSLK*RNENA CWHI

Frame 2

FVKLYVGTGSSNLSRAGLKLLG*SDPPASASQS AGIIGISHHALPIFLSWIILHSSLL*MRVPVVA YRNQFVIAYK*CAVMKLSLVSERIAKNFPSFLS FLLPLYCLLLKLVLDPGT*WGWTVQMAHMASVYW SISKELVCKF*CI*VEHRKEERCRLTYGPGAPA ILSHPYSPYLPALTLPSLSSGPSHQHIAGTWST SGKFISWLPPPRPAHAICSFPAPCHLKCSIMSKT SLLILKCRK*GWRREGEENQERFIEREDQKLLG TPYCRIKIFIMVFFLQNIPLYIIMFLHE*YKFK QEKYFLKSLRIFIKFRRPRISCVTWGMPLRL*AS ASLYVKWVEAALPHKHPVHRQCPAPLFSGLRGQ ACLGHTATYFWAVSSGNLLSCQCGQAL*TSMNQ ICFRLGLSTGEGGGKGHWGWECLLPSVSVTPVHP VPYLSLSGPTSFCCRLLPIE*QPSCFGFYFFVK SFPLQRTS*WV*KAAVATEAAERWPSAVPRLPT *VQLSPFLGLFKEVSRTDFRSLSMGPFSAPVEPG LL*TFRLASVEMTLPSTSTLGLKMEGTWCATRG RTEAGGPRRGRHTCLSRRGCPLTSASWCRAQIS R*W*TGSSSCSTSTACPSTVWTPSPSMALCSCPT SASRTPAQSLFSLPSPRCRSPSLSVSHPGPGGA DKNLPACGLPTRLPLPRQSSTQCRAPLDRCSLL PPSHL*CTPTPPIRCLSSPPFWEGCTHPSPSSCQ ALSCPVLRGSTSTCALGTTSPST*TPVLMRMLW SATPRSTTPGGLRSEVCPEKCPSSVARASQCGS CVKLTASRWPWMVSTCLNTTIA*GTCPPSTDWKW GATSS*PMCRHRRLPGPGAGGWGVGQSGSSHHP HFPGPAFPTLPGIWALMQRPCPCLVLLLATATL ERRRQLTGIAFLSRSSTWGSSCWNPTIPGGRHSQ GEGRSGQ*R*SPMLSPLPSPTQLHPSPKPPAVC SWWEVASSAPPL*PLTSLSPCTVHQPFTPPGKQ A*WLPTGLHHLTRVFSSEDWLLSQCP*NKEMKML VGTF