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Sequences

Sequence IGSF4.cAug05

GCAGCAGAGTGTGCCTGTGAGGCCAGGAGCGTGGAGCTCTCAAAGGACCATCTTGTTTTTCTGGTTTTAAACCAGGTGATGGGCAGAATCTGTTTACGAA
AGACGTGACAGTGATCGAGGGAGAGGTTGCGACCATCAGTTGCCAAGTCAATAAGAGTGACGACTCTGTGATTCAGCTACTGAATCCCAACAGGCAGACC
ATTTATTTCAGGGACTTCAGGCCTTTGAAGGACAGCAGGTTTCAGTTGCTGAATTTTTCTAGCAGTGAACTCAAAGTATCATTGACAAACGTCTCAATTT
CTGATGAAGGAAGATACTTTTGCCAGCTCTATACCGATCCCCCACAGGAAAGTTACACCACCATCACAGTCCTGGTCCCACCACGTAATCTGATGATCGA
TATCCAGAAAGACACTGCGGTGGAAGGTGAGGAGATTGAAGTCAACTGCACTGCTATGGCCAGCAAGCCAGCCACGACTATCAGGTGGTTCAAAGGGAAC
ACAGAGCTAAAAGGCAAATCGGAGGTGGAAGAGTGGTCAGACATGTACACTGTGACCAGTCAGCTGATGCTGAAGGTGCACAAGGAGGACGATGGGGTCC
CAGTGATCTGCCAGGTGGAGCACCCTGCGGTCACTGGAAACCTGCAGACCCAGCGGTATCTAGAAGTACAGTATAAGCCTCAAGTGCACATTCAGATGAC
TTATCCTCTACAAGGCTTAACCCGGGAAGGGGACGCGCTTGAGTTAACATGTGAAGCCATCGGGAAGCCCCAGCCTGTGATGGTAACTTGGGTGAGAGTC
GATGATGAAATGCCTCAACACGCCGTACTGTCTGGGCCCAACCTGTTCATCAATAACCTAAACAAAACAGATAATGGTACATACCGCTGTGAAGCTTCAA
ACATAGTGGGGAAAGCTCACTCGGATTATATGCTGTATGTATACGATCCCCCCACAACTATCCCTCCTCCCACAACAACCACCACCACCACCACCACCAC
CACCACCACCATCCTTACCATCATCACAGACACAACGGCGACGACAGAACCAGCAGTTCACGATTCCCGAGCAGGTGAAGAAGGCTCGATCAGGGCAGTG
GATCATGCCGTGATCGGTGGCGTCGTGGCGGTGGTGGTGTTCGCCATGCTGTGCTTGCTCATCATTCTGGGGCGCTATTTTGCCAGACATAAAGGTACAT
ACTTCACTCATGAAGCCAAAGGAGCCGATGACGCAGCAGACGCAGACACAGCTATAATCAATGCAGAAGGAGGACAGAACAACTCCGAAGAAAAGAAAGA
GTACTTCATCTAGATCAGCCTTTTTGTTTCAATGAGGTGTCCAACTGGCCCTATTTAGATGATAAAGAGACAGTGATATTGGAACTTGCGAGAAATTCGT
GTGTTTTTTTATGAATGGGTGGAAAGGTGTGAGACTGGGAAGGCTTGGGATTTGCTGTGTAAAAAAAAAAAAAATGTTCTTTGGAAAGTACACTCTGCTG
TTTGACACCTCTTTTTTCGTTTGTTTGTTTGTTTAATTTTTATTTCTTCCTACCAAGTCAAACTTGGATACTTGGATTTAGTTTCAGTAGATTGCAGAAA
ATTCTGTGCCTTGTTTTTTGTTTGTTTGTTGCGTTCCTTTCTTTTCCCCCTTTGTGCACATTTATTTCCTCCCTCTACCCCAATTTCGGATTTTTTCCAA
AATCTCCCATTTTGGAATTTGCCTGCTGGGATTCCTTAGACTCTTTTCCTTCCCTTTTCTGTTCTAGTTTTTTACTTTTGTTTATTTTTATGGTAACTGC
TTTCTGTTCCAAATTCAGTTTCATAAAAGGAGAACCAGCACAGCTTAGATTTCATAGTTCAGAATTTAGTGTATCCATAATGCATTCTTCTCTGTTGTCG
TAAAGATTTGGGTGAACAAACAATGAAAACTCTTTGCTGCTGCCCATGTTTCAAATACTTAGAGCAGTGAAGACTAGAAAATTAGACTGTGATTCAGAAA
ATGTTCTGTTTGCTGTGGAACTACATTACTGTACAGGGTTATCTGCAAGTGAGGTGTGTCACAATGAGATTGAATTTCACTGTCTTTAATTCTGTATCTG
TAGACGGCTCAGTATAGATACCCTACGCTGTCCAGAAAGGTTTGGGGCAGAAAGGACTCCTCCTTTTTCCATGCCCTAAACAGACCTGACAGGTGAGGTC
TGTTCCTTTTATATAAGTGGACAAATTTTGAGTTGCCACAGGAGGGGAAGTAGGGAGGGGGGAAATACAGTTCTGCTCTGGTTGTTTCTGTTCCAAATGA
TTCCATCCACCTTTCCCAATCGGCCTTACTTCTCACTAATTTGTAGGAAAAAGCAAGTTCGTCTGTTGTGCGAATGACTGAATGGGACAGAGTTGATTTT
TTTTTTTTTTTCCTTTGTGCTTAGTTAGGAAGGCAGTAGGATGTGGCCTGCATGTACTGTATATTACAGATATTTGTCATGCTGGGATTTCCAACTCGAA
TCTGTGTGAAACTTTCATTCCTTCAGATTTGGCTTGACAAAGGCAGGAGGTACAAAAGAAGGGCTGGTATTGTTCTCACACTGGTCTGCTGTCGCTCTCA
GTTCTCGATAGGTCAGAGCAGAGGTGGAAAAACAGCATGTACGGATTTTCAGTTACTTAATCAAAACTCAAATGTGAGTGTTTTTATCTTTTTACCTTTC
ATACACTAGCCTTGGCCTCTTTCCTCAGCCTTAAGAACCATCTGCCAAAAATTACTGATCCTCGCATGATGGCAGCCATAGTGCATAGCTACTAAAATCA
GTGACCTTGAACATATCTTAGATGGGGAGCCTCGGGAAAAGGTAGAGGAGTCACGTTACCATTTACATGTTTTAAAGAAAGAAGTGTGGGGATTTTCACT
GAAACGTCTAGGAAATCTAGAAGTAGTCCTGAAGGACAGAAACTAAACTCTTACCATATGTTTGGTAAGACTCCAGACTCCAGCTAACAGTCCCTATGGA
AAGATGGCATCAAAAAAGATAGATCTATATATATATATAAATATATATTCTATTACATTTTCAGTGAGTAATTTTGGATTTTGCAAGGTGCATTTTTACT
ATTGTTACATTATGTGGAAAACTTATGCTGATTTATTTAAGGGGGAAAAAGTGTCAACTCTTTGTTATTTGAAAACATGTTTATTTTTCTTGTCTTTATT
TTAACCTTTGATAGAACCATTGCAATATGGGGGCCTTTTGGGAACGGACTGGTATGTAAAAGAAAATCCATTATCGAGCAGCATTTTATTTACCCCTCCC
CTATCCCTAGGCACTTAACCAAGACAAAAAGCCACAATGAACATCCCTTTTTCAATGAATTTTATAATCTGCAGCTCTATTCCGAGCCCTTAGCACCCAT
TCCGACCATAGTATAATCATATCAAAGGGTGAGAATCATTTAGCATGTTGTTGAAAGGTTTTTTTTCAGTTGTTCTTTTTAGAAAAAAAGAAAAACAAAA
ACAAAAACAAAAAAAAAAAATCACACCATTGCTCACAGAATTGGCATCTCATTTTTGGGACCTCCCATCTTTCTGTTTTGAAAAGTGTACAGTAGTGCAG
TGTTCCTGATGTAACTTTATGGCTTACAATGTTGACATGTCTCAGGTTCATGTGTTGCGATTGGTGTTTTCCGTCTCAGGTAGATTGCAAAGTGTAGGCC
CCACACATTGGAAAAAATAATAATAAAACAAAGCAAAAACAGGAAATTATGGATTTTAGTTGTATATTGGTTTATGTATTTTTTCTTAAGTATACAGTGC
ACTGTTTGAAATGTATTGTTGAGTATTACTTTGTACAGGTTGATCACTTTTTTTAGAGTGAAGAAAGAACAAACTTGTTTTTTGTGTTTTTTAAAGGAAT
ATAAAATAATGAAGGATGTATAATTGATGCCAAATAAGCTTGTTCTTTAGTCACACCGACGTCTTATTTTTCCCTTTAGGCCAGTTCTGTTTTTAAGGTG
TACATGGACAATGTTACAGTGTAAGAAACTCCATATCCATATGTTCCCATTCGCATTTTGTATTGGTTCATGTATACCATTTTTACAAAAAAAAAAAGAA
AAAAAAGAAGTACTATAAAATATCTGTCTTCTTAATAAAAAAAAATTAATGTTACAAAGTGA
  • IGSF4.F6 (forward primer)
  • IGSF4.R1 (reverse primer)
  • Single exon skipping

Frame 0

AAECACEARSVELSKDHLVFLVLNQVMGRICLR
KT*Q*SRERLRPSVAKSIRVTTL*FSY*IPTGRP
FISGTSGL*RTAGFSC*IFLAVNSKYH*QTSQF
LMKEDTFASSIPIPHRKVTPPSQSWSHHVI**S
ISRKTLRWKVRRLKSTALLWPASQPRLSGGSKGT
QS*KANRRWKSGQTCTL*PVS*C*RCTRRTMGS
Q*SARWSTLRSLETCRPSGI*KYSISLKCTFR*
LILYKA*PGKGTRLS*HVKPSGSPSL*W*LG*ES
MMKCLNTPYCLGPTCSSIT*TKQIMVHTAVKLQ
T*WGKLTRIICCMYTIPPQLSLLPQQPPPPPPP
PPPPSLPSSQTQRRRQNQQFTIPEQVKKARSGQW
IMP*SVASWRWWCSPCCACSSFWGAILPDIKVH
TSLMKPKEPMTQQTQTQL*SMQKEDRTTPKKRK
STSSRSAFLFQ*GVQLALFR**RDSDIGTCEKFV
CFFMNGWKGVRLGRLGICCVKKKKMFFGKYTLL
FDTSFFVCLFV*FLFLPTKSNLDTWI*FQ*IAE
NSVPCFLFVCCVPFFSPFVHIYFLPLPQFRIFSK
ISHFGICLLGFLRLFSFPFLF*FFTFVYFYGNC
FLFQIQFHKRRTSTA*IS*FRI*CIHNAFFSVV
VKIWVNKQ*KLFAAAHVSNT*SSED*KIRL*FRK
CSVCCGTTLLYRVICK*GVSQ*D*ISLSLILYL
*TAQYRYPTLSRKVWGRKDSSFFHALNRPDR*G
LFLLYKWTNFELPQEGK*GGGKYSSALVVSVPND
SIHLSQSALLLTNL*EKASSSVVRMTEWDRVDF
FFFFLCA*LGRQ*DVACMYCILQIFVMLGFPTR
ICVKLSFLQIWLDKGRRYKRRAGIVLTLVCCRSQ
FSIGQSRGGKTACTDFQLLNQNSNVSVFIFLPF
IH*PWPLSSALRTICQKLLILA*WQP*CIATKI
SDLEHILDGEPREKVEESRYHLHVLKKEVWGFSL
KRLGNLEVVLKDRN*TLTICLVRLQTPANSPYG
KMASKKIDLYIYINIYSITFSVSNFGFCKVHFY
YCYIMWKTYADLFKGEKVSTLCYLKTCLFFLSLF
*PLIEPLQYGGLLGTDWYVKENPLSSSILFTPP
LSLGT*PRQKATMNIPFSMNFIICSSIPSP*HP
FRP*YNHIKG*ESFSMLLKGFFSVVLFRKKEKQK
QKQKKKITPLLTELASHFWDLPSFCFEKCTVVQ
CS*CNFMAYNVDMSQVHVLRLVFSVSGRLQSVG
PTHWKK***NKAKTGNYGF*LYIGLCIFS*VYSA
LFEMYC*VLLCTG*SLFLE*RKNKLVFCVF*RN
IK**RMYN*CQISLFFSHTDVLFFPLGQFCF*G
VHGQCYSVRNSISICSHSHFVLVHVYHFYKKKKK
KKKYYKISVFLIKKN*CYKV

Frame 1

QQSVPVRPGAWSSQRTILFFWF*TR*WAESVYE
RRDSDRGRGCDHQLPSQ*E*RLCDSATESQQAD
HLFQGLQAFEGQQVSVAEFF*Q*TQSIIDKRLNF
**RKILLPALYRSPTGKLHHHHSPGPTT*SDDR
YPERHCGGR*GD*SQLHCYGQQASHDYQVVQRE
HRAKRQIGGGRVVRHVHCDQSADAEGAQGGRWGP
SDLPGGAPCGHWKPADPAVSRSTV*ASSAHSDD
LSSTRLNPGRGRA*VNM*SHREAPACDGNLGES
R**NASTRRTVWAQPVHQ*PKQNR*WYIPL*SFK
HSGESSLGLYAVCIRSPHNYPSSHNNHHHHHHH
HHHHPYHHHRHNGDDRTSSSRFPSR*RRLDQGS
GSCRDRWRRGGGGVRHAVLAHHSGALFCQT*RYI
LHS*SQRSR*RSRRRHSYNQCRRRTEQLRRKER
VLHLDQPFCFNEVSNWPYLDDKETVILELARNS
CVFL*MGGKV*DWEGLGFAV*KKKKCSLESTLCC
LTPLFSFVCLFNFYFFLPSQTWILGFSFSRLQK
ILCLVFCLFVAFLSFPPLCTFISSLYPNFGFFP
KSPILEFACWDSLDSFPSLFCSSFLLLFIFMVTA
FCSKFSFIKGEPAQLRFHSSEFSVSIMHSSLLS
*RFG*TNNENSLLLPMFQILRAVKTRKLDCDSE
NVLFAVELHYCTGLSASEVCHNEIEFHCL*FCIC
RRLSIDTLRCPERFGAERTPPFSMP*TDLTGEV
CSFYISGQILSCHRRGSREGGNTVLLWLFLFQM
IPSTFPNRPYFSLICRKKQVRLLCE*LNGTELIF
FFFSFVLS*EGSRMWPACTVYYRYLSCWDFQLE
SV*NFHSFRFGLTKAGGTKEGLVLFSHWSAVAL
SSR*VRAEVEKQHVRIFSYLIKTQM*VFLSFYLS
YTSLGLFPQP*EPSAKNY*SSHDGSHSA*LLKS
VTLNIS*MGSLGKR*RSHVTIYMF*RKKCGDFH
*NV*EI*K*S*RTETKLLPYVW*DSRLQLTVPME
RWHQKR*IYIYI*IYILLHFQ*VILDFARCIFT
IVTLCGKLMLIYLRGKKCQLFVI*KHVYFSCLY
FNL**NHCNMGAFWERTGM*KKIHYRAAFYLPLP
YP*ALNQDKKPQ*TSLFQ*IL*SAALFRALSTH
SDHSIIISKGENHLACC*KVFFQLFFLEKKKNK
NKNKKKKSHHCSQNWHLIFGTSHLSVLKSVQ*CS
VPDVTLWLTMLTCLRFMCCDWCFPSQVDCKV*A
PHIGKNNNKTKQKQEIMDFSCILVYVFFLKYTV
HCLKCIVEYYFVQVDHFF*SEERTNLFFVFFKGI
*NNEGCIIDAK*ACSLVTPTSYFSL*ASSVFKV
YMDNVTV*ETPYPYVPIRILYWFMYTIFTKKKR
KKRSTIKYLSS**KKINVTK*

Frame 2

SRVCL*GQERGALKGPSCFSGFKPGDGQNLFTK
DVTVIEGEVATISCQVNKSDDSVIQLLNPNRQT
IYFRDFRPLKDSRFQLLNFSSSELKVSLTNVSI
SDEGRYFCQLYTDPPQESYTTITVLVPPRNLMID
IQKDTAVEGEEIEVNCTAMASKPATTIRWFKGN
TELKGKSEVEEWSDMYTVTSQLMLKVHKEDDGV
PVICQVEHPAVTGNLQTQRYLEVQYKPQVHIQMT
YPLQGLTREGDALELTCEAIGKPQPVMVTWVRV
DDEMPQHAVLSGPNLFINNLNKTDNGTYRCEAS
NIVGKAHSDYMLYVYDPPTTIPPPTTTTTTTTTT
TTTILTIITDTTATTEPAVHDSRAGEEGSIRAV
DHAVIGGVVAVVVFAMLCLLIILGRYFARHKGT
YFTHEAKGADDAADADTAIINAEGGQNNSEEKKE
YFI*ISLFVSMRCPTGPI*MIKRQ*YWNLREIR
VFFYEWVERCETGKAWDLLCKKKKNVLWKVHSA
V*HLFFRLFVCLIFISSYQVKLGYLDLVSVDCRK
FCALFFVCLLRSFLFPLCAHLFPPSTPISDFFQ
NLPFWNLPAGIP*TLFLPFSVLVFYFCLFLW*L
LSVPNSVS*KENQHSLDFIVQNLVYP*CILLCCR
KDLGEQTMKTLCCCPCFKYLEQ*RLEN*TVIQK
MFCLLWNYITVQGYLQVRCVTMRLNFTVFNSVS
VDGSV*IPYAVQKGLGQKGLLLFPCPKQT*QVRS
VPFI*VDKF*VATGGEVGRGEIQFCSGCFCSK*
FHPPFPIGLTSH*FVGKSKFVCCAND*MGQS*F
FFFFPLCLVRKAVGCGLHVLYITDICHAGISNSN
LCETFIPSDLA*QRQEVQKKGWYCSHTGLLSLS
VLDRSEQRWKNSMYGFSVT*SKLKCECFYLFTF
HTLALASFLSLKNHLPKITDPRMMAAIVHSY*NQ
*P*TYLRWGASGKGRGVTLPFTCFKERSVGIFT
ETSRKSRSSPEGQKLNSYHMFGKTPDSS*QSLW
KDGIKKDRSIYIYKYIFYYIFSE*FWILQGAFLL
LLHYVENLC*FI*GGKSVNSLLFENMFIFLVFI
LTFDRTIAIWGPFGNGLVCKRKSIIEQHFIYPS
PIPRHLTKTKSHNEHPFFNEFYNLQLYSEPLAPI
PTIV*SYQRVRII*HVVERFFFSCSF*KKRKTK
TKTKKKNHTIAHRIGISFLGPPIFLF*KVYSSA
VFLM*LYGLQC*HVSGSCVAIGVFRLR*IAKCRP
HTLEKIIIKQSKNRKLWILVVYWFMYFFLSIQC
TV*NVLLSITLYRLITFFRVKKEQTCFLCFLKE
YKIMKDV*LMPNKLVL*SHRRLIFPFRPVLFLRC
TWTMLQCKKLHIHMFPFAFCIGSCIPFLQKKKE
KKEVL*NICLLNKKKLMLQS

Sequence IGSF4.oAug05

AGAGCAGCACCACCTCCTCCTCCTCCTTCCTGCCATCTGTAAAAGCTGAAACGAAATGACAACCATTGTACCCTCTTGGTACTGAGTTGATTTAACCTCA
CCTTTGAAATCTGGTAGGGAAGAGGGGTGTCGTATGTTTTGATTTTTCCTCCCCTCTCTTCACATCTCCTCATCCCTAATTCACTTCATTAATGAAAATT
CTCTTTTCTTCTTTTCTCAGCTCTTCCAACTAAGGCTTTGGGATATAATTACAAGACATAAATCTTGCAGGGAAAGAACTTTTGGTTTTAAAGGGGGAAG
CCATCAAACAAACATCTCAATGTCCTCATGCCTTTGTTTTGTCATTACTGGTCATTGTTGTGTTCAGCATTGTGGGTTTGATTTTCCTTTTTTTTTCTAT
CCAGATCCCCCCACAACTATCCCTCCTCCCACAACAACCACCACCACCACCACCACCACCACCACCACCATCCTTACCATCATCACAGACACAACGGCGA
CGACAGAACCAGCAGTTCACGGCCTTACTCAGTTGCCCAATTCCGCAGAAGAACTGGACAGTGAGGACCTCTCAGATTCCCGAGCAGGTGAAGAAGGCTC
GATCAGGGCAGTGGATCATGCCGTGATCGGTGGCGTCGTGGCGGTGGTGGTGTTCGCCATGCTGTGCTTGCTCATCATTCTGGGGCGCTATTTTGCCAGA
CATAAAGGCCTGTTTTCTCTAACCTCCTCTCCCAGAATAAAGTGATGAGGGCAGCTATAGTGTTGCAATTAGGAACATGAATCTTTTATCGTTTGGACAA
GGAAGGGAAAATGCCATTACTGTCAACTGAAATGTCCTACTTGGGTTTTTTGCTCCTACTTCCCTGCCAAAGCCCATTGCTGCTGCTGAAATTGTATAAC
ATTCACAGCTGTTCTTGATGGCTTGATTCTTAACACAGCCAGAAATGTAGCCCGGGAGATGCTTAAGACAGTTTATGGAAAATAATAGATTTCCTTGCAG
AGACACCAATGCATCGAGAAGGGCCTTGAGTCACGTTAGCCCCAGCCAAGATCACCAATTTTCTCTCTATCTGGCTTCAGTTACAAAGGCTATGCCTT
  • IGSF4.F6 (forward primer)
  • IGSF4.R1 (reverse primer)
  • Single exon skipping

Frame 0

RAAPPPPPPSCHL*KLKRNDNHCTLLVLS*FNL
TFEIW*GRGVSYVLIFPPLSSHLLIPNSLH**KF
SFLLFSALPTKALGYNYKT*ILQGKNFWF*RGK
PSNKHLNVLMPLFCHYWSLLCSALWV*FSFFFL
SRSPHNYPSSHNNHHHHHHHHHHHPYHHHRHNGD
DRTSSSRPYSVAQFRRRTGQ*GPLRFPSR*RRL
DQGSGSCRDRWRRGGGGVRHAVLAHHSGALFCQ
T*RPVFSNLLSQNKVMRAAIVLQLGT*IFYRLDK
EGKMPLLSTEMSYLGFLLLLPCQSPLLLLKLYN
IHSCS*WLDS*HSQKCSPGDA*DSLWKIIDFLA
ETPMHREGP*VTLAPAKITNFLSIWLQLQRLCL

Frame 1

EQHHLLLLLPAICKS*NEMTTIVPSWY*VDLTS
PLKSGREEGCRMF*FFLPSLHISSSLIHFINEN
SLFFFSQLFQLRLWDIITRHKSCRERTFGFKGGS
HQTNISMSSCLCFVITGHCCVQHCGFDFPFFFY
PDPPTTIPPPTTTTTTTTTTTTTILTIITDTTA
TTEPAVHGLTQLPNSAEELDSEDLSDSRAGEEGS
IRAVDHAVIGGVVAVVVFAMLCLLIILGRYFAR
HKGLFSLTSSPRIK**GQL*CCN*EHESFIVWT
RKGKCHYCQLKCPTWVFCSYFPAKAHCCC*NCIT
FTAVLDGLILNTARNVAREMLKTVYGK**ISLQ
RHQCIEKGLESR*PQPRSPIFSLSGFSYKGYA

Frame 2

SSTTSSSSFLPSVKAETK*QPLYPLGTELI*PH
L*NLVGKRGVVCFDFSSPLFTSPHP*FTSLMKI
LFSSFLSSSN*GFGI*LQDINLAGKELLVLKGE
AIKQTSQCPHAFVLSLLVIVVFSIVGLIFLFFSI
QIPPQLSLLPQQPPPPPPPPPPPSLPSSQTQRR
RQNQQFTALLSCPIPQKNWTVRTSQIPEQVKKA
RSGQWIMP*SVASWRWWCSPCCACSSFWGAILPD
IKACFL*PPLPE*SDEGSYSVAIRNMNLLSFGQ
GRENAITVN*NVLLGFFAPTSLPKPIAAAEIV*
HSQLFLMA*FLTQPEM*PGRCLRQFMENNRFPCR
DTNASRRALSHVSPSQDHQFSLYLASVTKAMP

Sequence IGSF4.gAug05

GCCAGGATTACCAACTAATTAGAAGGGTGTTACTGAAGGCATCGGATGGGAGAGAGTAAGTGCTGGTACTGTGGTTTTGAGGCAGTGTGTGAGTCTGATT
TTGCCTGATGATATCATTTGCTAAACATTATACTTTGTGTTTTTGTCACACAGATAAGCCTCAAGTGCACATTCAGATGACTTATCCTCTACAAGGCTTA
ACCCGGGAAGGGGACGCGCTTGAGTTAACATGTGAAGCCATCGGGAAGCCCCAGCCTGTGATGGTAACTTGGGTGAGAGTCGATGATGAAATGCCTCAAC
ACGCCGTACTGTCTGGGCCCAACCTGTTCATCAATAACCTAAACAAAACAGATAATGGTACATACCGCTGTGAAGCTTCAAACATAGTGGGGAAAGCTCA
CTCGGATTATATGCTGTATGTATACGATCCCCCCACAACTATCCCTCCTCCCACAACAACCACCACCACCACCACCACCACCACCACCACCATCCTTACC
ATCATCACAGACACAACGGCGACGACAGAACCAGCAGTTCACGGCCTTACTCAGTTGCCCAATTCCGCAGAAGAACTGGACAGTGAGGACCTCTCAGATT
CCCGAGCAGGTGAAGAAGGCTCGATCAGGGCAGTGGATCATGCCGTGATCGGTGGCGTCGTGGCGGTGGTGGTGTTCGCCATGCTGTGCTTGCTCATCAT
TCTGGGGCGCTATTTTGCCAGACATAAAGGTACATACTTCACTCATGAAGCCAAAGGAGCCGATGACGCAGCAGACGCAGACACAGCTATAATCAATGCA
GAAGGAGGACAGAACAACTCCGAAGAAAAGAAAGAGTACTTCATCTAGATCAGCCTTTTTGTTTCAATGAGGTGTCCAACTGGCCCTATTTAGATGATAA
AGAGACAGTGATATTGGAACTTGCGAGAAATTCGTGTGTTTTTTTATGAATGGGTGGAAAGGTGTGAGACTGGGAAGGCTTGGGATTTGCTGTGTAAAAA
AAAAAAAAATGTTCTTTGGAAAGTACACTCTGCTGTTTGACACCTCTTTTTTCGTTTGTTTGTTTGTTTAATTTTTATTTCTTCCTACCAAGTCAAACTT
GGATACTTGGATTTAGTTTCAGTAGATTGCAGAAAATTCTGTGCCTTGTTTTTTGTTTGTTTGTTGCGTTCCTTTCTTTTCCCCCTTTGTGCACATTTAT
TTCCTCCCTCTACCCCAATTTCGGATTTTTTCCAAAATCTCCCATTTTGGAATTTGCCTGCTGGGATTCCTTAGACTCTTTTCCTTCCCTTTTCTGTTCT
AGTTTTTTACTTTTGTTTATTTTTATGGTAACTGCTTTCTGTTCCAAATTCAGTTTCATAAAAGGAGAACCAGCACAGCTTAGATTTCATAGTTCAGAAT
TTAGTGTATCCATAATGCATTCTTCTCTGTTGTCGTAAAGATTTGGGTGAACAAACAATGAAAACTCTTTGCTGCTGCCCATGTTTCAAATACTTAGAGC
AGTGAAGACTAGAAAATTAGACTGTGATTCAGAAAATGTTCTGTTTGCTGTGGAACTACATTACTGTACAGGGTTATCTGCAAGTGAGGTGTGTCACAAT
GAGATTGAATTTCACTGTCTTTAATTCTGTATCTGTAGACGGCTCAGTATAGATACCCTACGCTGTCCAGAAAGGTTTGGGGCAGAAAGGACTCCTCCTT
TTTCCATGCCCTAAACAGACCTGACAGGTGAGGTCTGTTCCTTTTATATAAGTGGACAAATTTTGAGTTGCCACAGGAGGGGAAGTAGGGAGGGGGGAAA
TACAGTTCTGCTCTGGTTGTTTCTGTTCCAAATGATTCCATCCACCTTTCCCAATCGGCCTTACTTCTCACTAATTTGTAGGAAAAAGCAAGTTCGTCTG
TTGTGCGAATGACTGAATGGGACAGAGTTGATTTTTTTTTTTTTTTCCTTTGTGCTTAGTTAGGAAGGCAGTAGGATGTGGCCTGCATGTACTGTATATT
ACAGATATTTGTCATGCTGGGATTTCCAACTCGAATCTGTGTGAAACTTTCATTCCTTCAGATTTGGCTTGACAAAGGCAGGAGGTACAAAAGAAGGGCT
GGTATTGTTCTCACACTGGTCTGCTGTCGCTCTCAGTTCTCGATAGGTCAGAGCAGAGGTGGAAAAACAGCATGTACGGATTTTCAGTTACTTAATCAAA
ACTCAAATGTGAGTGTTTTTATCTTTTTACCTTTCATACACTAGCCTTGGCCTCTTTCCTCAGCCTTAAGAACCATCTGCCAAAAATTACTGATCCTCGC
ATGATGGCAGCCATAGTGCATAGCTACTAAAATCAGTGACCTTGAACATATCTTAGATGGGGAGCCTCGGGAAAAGGTAGAGGAGTCACGTTACCATTTA
CATGTTTTAAAGAAAGAAGTGTGGGGATTTTCACTGAAACGTCTAGGAAATCTAGAAGTAGTCCTGAAGGACAGAAACTAAACTCTTACCATATGTTTGG
TAAGACTCCAGACTCCAGCTAACAGTCCCTATGGAAAGATGGCATCAAAAAAGATAGATCTATATATATATATAAATATATATTCTATTACATTTTCAGT
GAGTAATTTTGGATTTTGCAAGGTGCATTTTTACTATTGTTACATTATGTGGAAAACTTATGCTGATTTATTTAAGGGGGAAAAAGTGTCAACTCTTTGT
TATTTGAAAACATGTTTATTTTTCTTGTCTTTATTTTAACCTTTGATAGAACCATTGCAATATGGGGGCCTTTTGGGAACGGACTGGTATGTAAAAGAAA
ATCCATTATCGAGCAGCATTTTATTTACCCCTCCCCTATCCCTAGGCACTTAACCAAGACAAAAAGCCACAATGAACATCCCTTTTTCAATGAATTTTAT
AATCTGCAGCTCTATTCCGAGCCCTTAGCACCCATTCCGACCATAGTATAATCATATCAAAGGGTGAGAATCATTTAGCATGTTGTTGAAAGGTTTTTTT
TCAGTTGTTCTTTTTAGAAAAAAAGAAAAACAAAAACAAAAACAAAAAAAAAAAATCACACCATTGCTCACAGAATTGGCATCTCATTTTTGGGACCTCC
CATCTTTCTGTTTTGAAAAGTGTACAGTAGTGCAGTGTTCCTGATGTAACTTTATGGCTTACAATGTTGACATGTCTCAGGTTCATGTGTTGCGATTGGT
GTTTTCCGTCTCAGGTAGATTGCAAAGTGTAGGCCCCACACATTGGAAAAAATAATAATAAAACAAAGCAAAAACAGGAAATTATGGATTTTAGTTGTAT
ATTGGTTTATGTATTTTTTCTTAAGTATACAGTGCACTGTTTGAAATGTATTGTTGAGTATTACTTTGTACAGGTTGATCACTTTTTTTAGAGTGAAGAA
AGAACAAACTTGTTTTTTGTGTTTTTTAAAGGAATATAAAATAATGAAGGATGTATAATTGATGCCAAATAAGCTTGTTCTTTAGTCACACCGACGTCTT
ATTTTTCCCTTTAGGCCAGTTCTGTTTTTAAGGTGTACATGGACAATGTTACAGTGTAAGAAACTCCATATCCATATGTTCCCATTCGCATTTTGTATTG
GTTCATGTATACCATTTTTACAAAAAAAAAAAGAAAAAAAAGAAGTACTATAAAATATCTGTCTTCTTAATAAAAAAAAATTAATGTTACAAAGTGA
  • IGSF4.F6 (forward primer)
  • IGSF4.R1 (reverse primer)
  • Single exon skipping

Frame 0

ARITN*LEGCY*RHRMGESKCWYCGFEAVCESD
FA**YHLLNIILCVFVTQISLKCTFR*LILYKA*
PGKGTRLS*HVKPSGSPSL*W*LG*ESMMKCLN
TPYCLGPTCSSIT*TKQIMVHTAVKLQT*WGKL
TRIICCMYTIPPQLSLLPQQPPPPPPPPPPPSLP
SSQTQRRRQNQQFTALLSCPIPQKNWTVRTSQI
PEQVKKARSGQWIMP*SVASWRWWCSPCCACSS
FWGAILPDIKVHTSLMKPKEPMTQQTQTQL*SMQ
KEDRTTPKKRKSTSSRSAFLFQ*GVQLALFR**
RDSDIGTCEKFVCFFMNGWKGVRLGRLGICCVK
KKKMFFGKYTLLFDTSFFVCLFV*FLFLPTKSNL
DTWI*FQ*IAENSVPCFLFVCCVPFFSPFVHIY
FLPLPQFRIFSKISHFGICLLGFLRLFSFPFLF
*FFTFVYFYGNCFLFQIQFHKRRTSTA*IS*FRI
*CIHNAFFSVVVKIWVNKQ*KLFAAAHVSNT*S
SED*KIRL*FRKCSVCCGTTLLYRVICK*GVSQ
*D*ISLSLILYL*TAQYRYPTLSRKVWGRKDSSF
FHALNRPDR*GLFLLYKWTNFELPQEGK*GGGK
YSSALVVSVPNDSIHLSQSALLLTNL*EKASSS
VVRMTEWDRVDFFFFFLCA*LGRQ*DVACMYCIL
QIFVMLGFPTRICVKLSFLQIWLDKGRRYKRRA
GIVLTLVCCRSQFSIGQSRGGKTACTDFQLLNQ
NSNVSVFIFLPFIH*PWPLSSALRTICQKLLILA
*WQP*CIATKISDLEHILDGEPREKVEESRYHL
HVLKKEVWGFSLKRLGNLEVVLKDRN*TLTICL
VRLQTPANSPYGKMASKKIDLYIYINIYSITFSV
SNFGFCKVHFYYCYIMWKTYADLFKGEKVSTLC
YLKTCLFFLSLF*PLIEPLQYGGLLGTDWYVKE
NPLSSSILFTPPLSLGT*PRQKATMNIPFSMNFI
ICSSIPSP*HPFRP*YNHIKG*ESFSMLLKGFF
SVVLFRKKEKQKQKQKKKITPLLTELASHFWDL
PSFCFEKCTVVQCS*CNFMAYNVDMSQVHVLRLV
FSVSGRLQSVGPTHWKK***NKAKTGNYGF*LY
IGLCIFS*VYSALFEMYC*VLLCTG*SLFLE*R
KNKLVFCVF*RNIK**RMYN*CQISLFFSHTDVL
FFPLGQFCF*GVHGQCYSVRNSISICSHSHFVL
VHVYHFYKKKKKKKKYYKISVFLIKKN*CYKV

Frame 1

PGLPTN*KGVTEGIGWERVSAGTVVLRQCVSLI
LPDDIIC*TLYFVFLSHR*ASSAHSDDLSSTRL
NPGRGRA*VNM*SHREAPACDGNLGESR**NAST
RRTVWAQPVHQ*PKQNR*WYIPL*SFKHSGESS
LGLYAVCIRSPHNYPSSHNNHHHHHHHHHHHPY
HHHRHNGDDRTSSSRPYSVAQFRRRTGQ*GPLRF
PSR*RRLDQGSGSCRDRWRRGGGGVRHAVLAHH
SGALFCQT*RYILHS*SQRSR*RSRRRHSYNQC
RRRTEQLRRKERVLHLDQPFCFNEVSNWPYLDDK
ETVILELARNSCVFL*MGGKV*DWEGLGFAV*K
KKKCSLESTLCCLTPLFSFVCLFNFYFFLPSQT
WILGFSFSRLQKILCLVFCLFVAFLSFPPLCTFI
SSLYPNFGFFPKSPILEFACWDSLDSFPSLFCS
SFLLLFIFMVTAFCSKFSFIKGEPAQLRFHSSE
FSVSIMHSSLLS*RFG*TNNENSLLLPMFQILRA
VKTRKLDCDSENVLFAVELHYCTGLSASEVCHN
EIEFHCL*FCICRRLSIDTLRCPERFGAERTPP
FSMP*TDLTGEVCSFYISGQILSCHRRGSREGGN
TVLLWLFLFQMIPSTFPNRPYFSLICRKKQVRL
LCE*LNGTELIFFFFSFVLS*EGSRMWPACTVY
YRYLSCWDFQLESV*NFHSFRFGLTKAGGTKEGL
VLFSHWSAVALSSR*VRAEVEKQHVRIFSYLIK
TQM*VFLSFYLSYTSLGLFPQP*EPSAKNY*SS
HDGSHSA*LLKSVTLNIS*MGSLGKR*RSHVTIY
MF*RKKCGDFH*NV*EI*K*S*RTETKLLPYVW
*DSRLQLTVPMERWHQKR*IYIYI*IYILLHFQ
*VILDFARCIFTIVTLCGKLMLIYLRGKKCQLFV
I*KHVYFSCLYFNL**NHCNMGAFWERTGM*KK
IHYRAAFYLPLPYP*ALNQDKKPQ*TSLFQ*IL
*SAALFRALSTHSDHSIIISKGENHLACC*KVFF
QLFFLEKKKNKNKNKKKKSHHCSQNWHLIFGTS
HLSVLKSVQ*CSVPDVTLWLTMLTCLRFMCCDW
CFPSQVDCKV*APHIGKNNNKTKQKQEIMDFSCI
LVYVFFLKYTVHCLKCIVEYYFVQVDHFF*SEE
RTNLFFVFFKGI*NNEGCIIDAK*ACSLVTPTS
YFSL*ASSVFKVYMDNVTV*ETPYPYVPIRILYW
FMYTIFTKKKRKKRSTIKYLSS**KKINVTK*

Frame 2

QDYQLIRRVLLKASDGRE*VLVLWF*GSV*V*F
CLMISFAKHYTLCFCHTDKPQVHIQMTYPLQGL
TREGDALELTCEAIGKPQPVMVTWVRVDDEMPQ
HAVLSGPNLFINNLNKTDNGTYRCEASNIVGKAH
SDYMLYVYDPPTTIPPPTTTTTTTTTTTTTILT
IITDTTATTEPAVHGLTQLPNSAEELDSEDLSD
SRAGEEGSIRAVDHAVIGGVVAVVVFAMLCLLII
LGRYFARHKGTYFTHEAKGADDAADADTAIINA
EGGQNNSEEKKEYFI*ISLFVSMRCPTGPI*MI
KRQ*YWNLREIRVFFYEWVERCETGKAWDLLCKK
KKNVLWKVHSAV*HLFFRLFVCLIFISSYQVKL
GYLDLVSVDCRKFCALFFVCLLRSFLFPLCAHL
FPPSTPISDFFQNLPFWNLPAGIP*TLFLPFSVL
VFYFCLFLW*LLSVPNSVS*KENQHSLDFIVQN
LVYP*CILLCCRKDLGEQTMKTLCCCPCFKYLE
Q*RLEN*TVIQKMFCLLWNYITVQGYLQVRCVTM
RLNFTVFNSVSVDGSV*IPYAVQKGLGQKGLLL
FPCPKQT*QVRSVPFI*VDKF*VATGGEVGRGE
IQFCSGCFCSK*FHPPFPIGLTSH*FVGKSKFVC
CAND*MGQS*FFFFFPLCLVRKAVGCGLHVLYI
TDICHAGISNSNLCETFIPSDLA*QRQEVQKKG
WYCSHTGLLSLSVLDRSEQRWKNSMYGFSVT*SK
LKCECFYLFTFHTLALASFLSLKNHLPKITDPR
MMAAIVHSY*NQ*P*TYLRWGASGKGRGVTLPF
TCFKERSVGIFTETSRKSRSSPEGQKLNSYHMFG
KTPDSS*QSLWKDGIKKDRSIYIYKYIFYYIFS
E*FWILQGAFLLLLHYVENLC*FI*GGKSVNSL
LFENMFIFLVFILTFDRTIAIWGPFGNGLVCKRK
SIIEQHFIYPSPIPRHLTKTKSHNEHPFFNEFY
NLQLYSEPLAPIPTIV*SYQRVRII*HVVERFF
FSCSF*KKRKTKTKTKKKNHTIAHRIGISFLGPP
IFLF*KVYSSAVFLM*LYGLQC*HVSGSCVAIG
VFRLR*IAKCRPHTLEKIIIKQSKNRKLWILVV
YWFMYFFLSIQCTV*NVLLSITLYRLITFFRVKK
EQTCFLCFLKEYKIMKDV*LMPNKLVL*SHRRL
IFPFRPVLFLRCTWTMLQCKKLHIHMFPFAFCI
GSCIPFLQKKKEKKEVL*NICLLNKKKLMLQS

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