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Sequences

Sequence IGSF4.aAug05

GAACGCCAGCGCCAGGGGGCGGGGTGGGGGAGGGAGCGAGGCCCTCCGAGAGCCGGGTTGGGCTCGCGGCGCTGTGATTGGTCTGCCCGGACTCCGCCTC CAGCGCATGTCATTAGCATCTCATTAGCTGTCCGCTCGGGCTCCGGAGGCAGCCAACGCCGCCAGTCTGAGGCAGGTGCCCGACATGGCGAGTGTAGTGC TGCCGAGCGGATCCCAGTGTGCGGCGGCAGCGGCGGCGGCGGCGCCTCCCGGGCTCCGGCTCCGGCTTCTGCTGTTGCTCTTCTCCGCCGCGGCACTGAT CCCCACAGGTGATGGGCAGAATCTGTTTACGAAAGACGTGACAGTGATCGAGGGAGAGGTTGCGACCATCAGTTGCCAAGTCAATAAGAGTGACGACTCT GTGATTCAGCTACTGAATCCCAACAGGCAGACCATTTATTTCAGGGACTTCAGGCCTTTGAAGGACAGCAGGTTTCAGTTGCTGAATTTTTCTAGCAGTG AACTCAAAGTATCATTGACAAACGTCTCAATTTCTGATGAAGGAAGATACTTTTGCCAGCTCTATACCGATCCCCCACAGGAAAGTTACACCACCATCAC AGTCCTGGTCCCACCACGTAATCTGATGATCGATATCCAGAAAGACACTGCGGTGGAAGGTGAGGAGATTGAAGTCAACTGCACTGCTATGGCCAGCAAG CCAGCCACGACTATCAGGTGGTTCAAAGGGAACACAGAGCTAAAAGGCAAATCGGAGGTGGAAGAGTGGTCAGACATGTACACTGTGACCAGTCAGCTGA TGCTGAAGGTGCACAAGGAGGACGATGGGGTCCCAGTGATCTGCCAGGTGGAGCACCCTGCGGTCACTGGAAACCTGCAGACCCAGCGGTATCTAGAAGT ACAGTATAAGCCTCAAGTGCACATTCAGATGACTTATCCTCTACAAGGCTTAACCCGGGAAGGGGACGCGCTTGAGTTAACATGTGAAGCCATCGGGAAG CCCCAGCCTGTGATGGTAACTTGGGTGAGAGTCGATGATGAAATGCCTCAACACGCCGTACTGTCTGGGCCCAACCTGTTCATCAATAACCTAAACAAAA CAGATAATGGTACATACCGCTGTGAAGCTTCAAACATAGTGGGGAAAGCTCACTCGGATTATATGCTGTATGTATACGATCCCCCCACAACTATCCCTCC TCCCACAACAACCACCACCACCACCACCACCACCACCACCACCATCCTTACCATCATCACAGATTCCCGAGCAGGTGAAGAAGGCTCGATCAGGGCAGTG GATCATGCCGTGATCGGTGGCGTCGTGGCGGTGGTGGTGTTCGCCATGCTGTGCTTGCTCATCATTCTGGGGCGCTATTTTGCCAGACATAAAGGTACAT ACTTCACTCATGAAGCCAAAGGAGCCGATGACGCAGCAGACGCAGACACAGCTATAATCAATGCAGAAGGAGGACAGAACAACTCCGAAGAAAAGAAAGA GTACTTCATCTAGATCAGCCTTTTTGTTTCAATGAGGTGTCCAACTGGCCCTATTTAGATGATAAAGAGACAGTGATATTGGAACTTGCGAGAAATTCGT GTGTTTTTTTATGAATGGGTGGAAAGGTGTGAGACTGGGAAGGCTTGGGATTTGCTGTGTAAAAAAAAAAAAAATGTTCTTTGGAAAGTACACTCTGCTG TTTGACACCTCTTTTTTCGTTTGTTTGTTTGTTTAATTTTTATTTCTTCCTACCAAGTCAAACTTGGATACTTGGATTTAGTTTCAGTAGATTGCAGAAA ATTCTGTGCCTTGTTTTTTGTTTGTTTGTTGCGTTCCTTTCTTTTCCCCCTTTGTGCACATTTATTTCCTCCCTCTACCCCAATTTCGGATTTTTTCCAA AATCTCCCATTTTGGAATTTGCCTGCTGGGATTCCTTAGACTCTTTTCCTTCCCTTTTCTGTTCTAGTTTTTTACTTTTGTTTATTTTTATGGTAACTGC TTTCTGTTCCAAATTCAGTTTCATAAAAGGAGAACCAGCACAGCTTAGATTTCATAGTTCAGAATTTAGTGTATCCATAATGCATTCTTCTCTGTTGTCG TAAAGATTTGGGTGAACAAACAATGAAAACTCTTTGCTGCTGCCCATGTTTCAAATACTTAGAGCAGTGAAGACTAGAAAATTAGACTGTGATTCAGAAA ATGTTCTGTTTGCTGTGGAACTACATTACTGTACAGGGTTATCTGCAAGTGAGGTGTGTCACAATGAGATTGAATTTCACTGTCTTTAATTCTGTATCTG TAGACGGCTCAGTATAGATACCCTACGCTGTCCAGAAAGGTTTGGGGCAGAAAGGACTCCTCCTTTTTCCATGCCCTAAACAGACCTGACAGGTGAGGTC TGTTCCTTTTATATAAGTGGACAAATTTTGAGTTGCCACAGGAGGGGAAGTAGGGAGGGGGGAAATACAGTTCTGCTCTGGTTGTTTCTGTTCCAAATGA TTCCATCCACCTTTCCCAATCGGCCTTACTTCTCACTAATTTGTAGGAAAAAGCAAGTTCGTCTGTTGTGCGAATGACTGAATGGGACAGAGTTGATTTT TTTTTTTTTTTCCTTTGTGCTTAGTTAGGAAGGCAGTAGGATGTGGCCTGCATGTACTGTATATTACAGATATTTGTCATGCTGGGATTTCCAACTCGAA TCTGTGTGAAACTTTCATTCCTTCAGATTTGGCTTGACAAAGGCAGGAGGTACAAAAGAAGGGCTGGTATTGTTCTCACACTGGTCTGCTGTCGCTCTCA GTTCTCGATAGGTCAGAGCAGAGGTGGAAAAACAGCATGTACGGATTTTCAGTTACTTAATCAAAACTCAAATGTGAGTGTTTTTATCTTTTTACCTTTC ATACACTAGCCTTGGCCTCTTTCCTCAGCCTTAAGAACCATCTGCCAAAAATTACTGATCCTCGCATGATGGCAGCCATAGTGCATAGCTACTAAAATCA GTGACCTTGAACATATCTTAGATGGGGAGCCTCGGGAAAAGGTAGAGGAGTCACGTTACCATTTACATGTTTTAAAGAAAGAAGTGTGGGGATTTTCACT GAAACGTCTAGGAAATCTAGAAGTAGTCCTGAAGGACAGAAACTAAACTCTTACCATATGTTTGGTAAGACTCCAGACTCCAGCTAACAGTCCCTATGGA AAGATGGCATCAAAAAAGATAGATCTATATATATATATAAATATATATTCTATTACATTTTCAGTGAGTAATTTTGGATTTTGCAAGGTGCATTTTTACT ATTGTTACATTATGTGGAAAACTTATGCTGATTTATTTAAGGGGGAAAAAGTGTCAACTCTTTGTTATTTGAAAACATGTTTATTTTTCTTGTCTTTATT TTAACCTTTGATAGAACCATTGCAATATGGGGGCCTTTTGGGAACGGACTGGTATGTAAAAGAAAATCCATTATCGAGCAGCATTTTATTTACCCCTCCC CTATCCCTAGGCACTTAACCAAGACAAAAAGCCACAATGAACATCCCTTTTTCAATGAATTTTATAATCTGCAGCTCTATTCCGAGCCCTTAGCACCCAT TCCGACCATAGTATAATCATATCAAAGGGTGAGAATCATTTAGCATGTTGTTGAAAGGTTTTTTTTCAGTTGTTCTTTTTAGAAAAAAAGAAAAACAAAA ACAAAAACAAAAAAAAAAAATCACACCATTGCTCACAGAATTGGCATCTCATTTTTGGGACCTCCCATCTTTCTGTTTTGAAAAGTGTACAGTAGTGCAG TGTTCCTGATGTAACTTTATGGCTTACAATGTTGACATGTCTCAGGTTCATGTGTTGCGATTGGTGTTTTCCGTCTCAGGTAGATTGCAAAGTGTAGGCC CCACACATTGGAAAAAATAATAATAAAACAAAGCAAAAACAGGAAATTATGGATTTTAGTTGTATATTGGTTTATGTATTTTTTCTTAAGTATACAGTGC ACTGTTTGAAATGTATTGTTGAGTATTACTTTGTACAGGTTGATCACTTTTTTTAGAGTGAAGAAAGAACAAACTTGTTTTTTGTGTTTTTTAAAGGAAT ATAAAATAATGAAGGATGTATAATTGATGCCAAATAAGCTTGTTCTTTAGTCACACCGACGTCTTATTTTTCCCTTTAGGCCAGTTCTGTTTTTAAGGTG TACATGGACAATGTTACAGTGTAAGAAACTCCATATCCATATGTTCCCATTCGCATTTTGTATTGGTTCATGTATACCATTTTTACAAAAAAAAAAAGAA AAAAAAGAAGTACTATAAAATATCTGTCTTCTTAATAAAAAAAAATTAATGTTACAAAGTGA

IGSF4.F6 (forward primer)
IGSF4.R1 (reverse primer)
Single exon skipping

Frame 0

ERQRQGAGWGRERGPPRAGLGSRRCDWSARTPP PAHVISISLAVRSGSGGSQRRQSEAGARHGECSA AERIPVCGGSGGGGASRAPAPASAVALLRRGTD PHR*WAESVYERRDSDRGRGCDHQLPSQ*E*RL CDSATESQQADHLFQGLQAFEGQQVSVAEFF*Q* TQSIIDKRLNF**RKILLPALYRSPTGKLHHHH SPGPTT*SDDRYPERHCGGR*GD*SQLHCYGQQ ASHDYQVVQREHRAKRQIGGGRVVRHVHCDQSAD AEGAQGGRWGPSDLPGGAPCGHWKPADPAVSRS TV*ASSAHSDDLSSTRLNPGRGRA*VNM*SHRE APACDGNLGESR**NASTRRTVWAQPVHQ*PKQN R*WYIPL*SFKHSGESSLGLYAVCIRSPHNYPS SHNNHHHHHHHHHHHPYHHHRFPSR*RRLDQGS GSCRDRWRRGGGGVRHAVLAHHSGALFCQT*RYI LHS*SQRSR*RSRRRHSYNQCRRRTEQLRRKER VLHLDQPFCFNEVSNWPYLDDKETVILELARNS CVFL*MGGKV*DWEGLGFAV*KKKKCSLESTLCC LTPLFSFVCLFNFYFFLPSQTWILGFSFSRLQK ILCLVFCLFVAFLSFPPLCTFISSLYPNFGFFP KSPILEFACWDSLDSFPSLFCSSFLLLFIFMVTA FCSKFSFIKGEPAQLRFHSSEFSVSIMHSSLLS *RFG*TNNENSLLLPMFQILRAVKTRKLDCDSE NVLFAVELHYCTGLSASEVCHNEIEFHCL*FCIC RRLSIDTLRCPERFGAERTPPFSMP*TDLTGEV CSFYISGQILSCHRRGSREGGNTVLLWLFLFQM IPSTFPNRPYFSLICRKKQVRLLCE*LNGTELIF FFFSFVLS*EGSRMWPACTVYYRYLSCWDFQLE SV*NFHSFRFGLTKAGGTKEGLVLFSHWSAVAL SSR*VRAEVEKQHVRIFSYLIKTQM*VFLSFYLS YTSLGLFPQP*EPSAKNY*SSHDGSHSA*LLKS VTLNIS*MGSLGKR*RSHVTIYMF*RKKCGDFH *NV*EI*K*S*RTETKLLPYVW*DSRLQLTVPME RWHQKR*IYIYI*IYILLHFQ*VILDFARCIFT IVTLCGKLMLIYLRGKKCQLFVI*KHVYFSCLY FNL**NHCNMGAFWERTGM*KKIHYRAAFYLPLP YP*ALNQDKKPQ*TSLFQ*IL*SAALFRALSTH SDHSIIISKGENHLACC*KVFFQLFFLEKKKNK NKNKKKKSHHCSQNWHLIFGTSHLSVLKSVQ*CS VPDVTLWLTMLTCLRFMCCDWCFPSQVDCKV*A PHIGKNNNKTKQKQEIMDFSCILVYVFFLKYTV HCLKCIVEYYFVQVDHFF*SEERTNLFFVFFKGI *NNEGCIIDAK*ACSLVTPTSYFSL*ASSVFKV YMDNVTV*ETPYPYVPIRILYWFMYTIFTKKKR KKRSTIKYLSS**KKINVTK*

Frame 1

NASARGRGGGGSEALREPGWARGAVIGLPGLRL QRMSLASH*LSARAPEAANAASLRQVPDMASVV LPSGSQCAAAAAAAAPPGLRLRLLLLLFSAAALI PTGDGQNLFTKDVTVIEGEVATISCQVNKSDDS VIQLLNPNRQTIYFRDFRPLKDSRFQLLNFSSS ELKVSLTNVSISDEGRYFCQLYTDPPQESYTTIT VLVPPRNLMIDIQKDTAVEGEEIEVNCTAMASK PATTIRWFKGNTELKGKSEVEEWSDMYTVTSQL MLKVHKEDDGVPVICQVEHPAVTGNLQTQRYLEV QYKPQVHIQMTYPLQGLTREGDALELTCEAIGK PQPVMVTWVRVDDEMPQHAVLSGPNLFINNLNK TDNGTYRCEASNIVGKAHSDYMLYVYDPPTTIPP PTTTTTTTTTTTTTILTIITDSRAGEEGSIRAV DHAVIGGVVAVVVFAMLCLLIILGRYFARHKGT YFTHEAKGADDAADADTAIINAEGGQNNSEEKKE YFI*ISLFVSMRCPTGPI*MIKRQ*YWNLREIR VFFYEWVERCETGKAWDLLCKKKKNVLWKVHSA V*HLFFRLFVCLIFISSYQVKLGYLDLVSVDCRK FCALFFVCLLRSFLFPLCAHLFPPSTPISDFFQ NLPFWNLPAGIP*TLFLPFSVLVFYFCLFLW*L LSVPNSVS*KENQHSLDFIVQNLVYP*CILLCCR KDLGEQTMKTLCCCPCFKYLEQ*RLEN*TVIQK MFCLLWNYITVQGYLQVRCVTMRLNFTVFNSVS VDGSV*IPYAVQKGLGQKGLLLFPCPKQT*QVRS VPFI*VDKF*VATGGEVGRGEIQFCSGCFCSK* FHPPFPIGLTSH*FVGKSKFVCCAND*MGQS*F FFFFPLCLVRKAVGCGLHVLYITDICHAGISNSN LCETFIPSDLA*QRQEVQKKGWYCSHTGLLSLS VLDRSEQRWKNSMYGFSVT*SKLKCECFYLFTF HTLALASFLSLKNHLPKITDPRMMAAIVHSY*NQ *P*TYLRWGASGKGRGVTLPFTCFKERSVGIFT ETSRKSRSSPEGQKLNSYHMFGKTPDSS*QSLW KDGIKKDRSIYIYKYIFYYIFSE*FWILQGAFLL LLHYVENLC*FI*GGKSVNSLLFENMFIFLVFI LTFDRTIAIWGPFGNGLVCKRKSIIEQHFIYPS PIPRHLTKTKSHNEHPFFNEFYNLQLYSEPLAPI PTIV*SYQRVRII*HVVERFFFSCSF*KKRKTK TKTKKKNHTIAHRIGISFLGPPIFLF*KVYSSA VFLM*LYGLQC*HVSGSCVAIGVFRLR*IAKCRP HTLEKIIIKQSKNRKLWILVVYWFMYFFLSIQC TV*NVLLSITLYRLITFFRVKKEQTCFLCFLKE YKIMKDV*LMPNKLVL*SHRRLIFPFRPVLFLRC TWTMLQCKKLHIHMFPFAFCIGSCIPFLQKKKE KKEVL*NICLLNKKKLMLQS

Frame 2

TPAPGGGVGEGARPSESRVGLAAL*LVCPDSAS SACH*HLISCPLGLRRQPTPPV*GRCPTWRV*C CRADPSVRRQRRRRRLPGSGSGFCCCSSPPRH* SPQVMGRICLRKT*Q*SRERLRPSVAKSIRVTTL *FSY*IPTGRPFISGTSGL*RTAGFSC*IFLAV NSKYH*QTSQFLMKEDTFASSIPIPHRKVTPPS QSWSHHVI**SISRKTLRWKVRRLKSTALLWPAS QPRLSGGSKGTQS*KANRRWKSGQTCTL*PVS* C*RCTRRTMGSQ*SARWSTLRSLETCRPSGI*K YSISLKCTFR*LILYKA*PGKGTRLS*HVKPSGS PSL*W*LG*ESMMKCLNTPYCLGPTCSSIT*TK QIMVHTAVKLQT*WGKLTRIICCMYTIPPQLSL LPQQPPPPPPPPPPPSLPSSQIPEQVKKARSGQW IMP*SVASWRWWCSPCCACSSFWGAILPDIKVH TSLMKPKEPMTQQTQTQL*SMQKEDRTTPKKRK STSSRSAFLFQ*GVQLALFR**RDSDIGTCEKFV CFFMNGWKGVRLGRLGICCVKKKKMFFGKYTLL FDTSFFVCLFV*FLFLPTKSNLDTWI*FQ*IAE NSVPCFLFVCCVPFFSPFVHIYFLPLPQFRIFSK ISHFGICLLGFLRLFSFPFLF*FFTFVYFYGNC FLFQIQFHKRRTSTA*IS*FRI*CIHNAFFSVV VKIWVNKQ*KLFAAAHVSNT*SSED*KIRL*FRK CSVCCGTTLLYRVICK*GVSQ*D*ISLSLILYL *TAQYRYPTLSRKVWGRKDSSFFHALNRPDR*G LFLLYKWTNFELPQEGK*GGGKYSSALVVSVPND SIHLSQSALLLTNL*EKASSSVVRMTEWDRVDF FFFFLCA*LGRQ*DVACMYCILQIFVMLGFPTR ICVKLSFLQIWLDKGRRYKRRAGIVLTLVCCRSQ FSIGQSRGGKTACTDFQLLNQNSNVSVFIFLPF IH*PWPLSSALRTICQKLLILA*WQP*CIATKI SDLEHILDGEPREKVEESRYHLHVLKKEVWGFSL KRLGNLEVVLKDRN*TLTICLVRLQTPANSPYG KMASKKIDLYIYINIYSITFSVSNFGFCKVHFY YCYIMWKTYADLFKGEKVSTLCYLKTCLFFLSLF *PLIEPLQYGGLLGTDWYVKENPLSSSILFTPP LSLGT*PRQKATMNIPFSMNFIICSSIPSP*HP FRP*YNHIKG*ESFSMLLKGFFSVVLFRKKEKQK QKQKKKITPLLTELASHFWDLPSFCFEKCTVVQ CS*CNFMAYNVDMSQVHVLRLVFSVSGRLQSVG PTHWKK***NKAKTGNYGF*LYIGLCIFS*VYSA LFEMYC*VLLCTG*SLFLE*RKNKLVFCVF*RN IK**RMYN*CQISLFFSHTDVLFFPLGQFCF*G VHGQCYSVRNSISICSHSHFVLVHVYHFYKKKKK KKKYYKISVFLIKKN*CYKV

Sequence IGSF4.cAug05

GCAGCAGAGTGTGCCTGTGAGGCCAGGAGCGTGGAGCTCTCAAAGGACCATCTTGTTTTTCTGGTTTTAAACCAGGTGATGGGCAGAATCTGTTTACGAA AGACGTGACAGTGATCGAGGGAGAGGTTGCGACCATCAGTTGCCAAGTCAATAAGAGTGACGACTCTGTGATTCAGCTACTGAATCCCAACAGGCAGACC ATTTATTTCAGGGACTTCAGGCCTTTGAAGGACAGCAGGTTTCAGTTGCTGAATTTTTCTAGCAGTGAACTCAAAGTATCATTGACAAACGTCTCAATTT CTGATGAAGGAAGATACTTTTGCCAGCTCTATACCGATCCCCCACAGGAAAGTTACACCACCATCACAGTCCTGGTCCCACCACGTAATCTGATGATCGA TATCCAGAAAGACACTGCGGTGGAAGGTGAGGAGATTGAAGTCAACTGCACTGCTATGGCCAGCAAGCCAGCCACGACTATCAGGTGGTTCAAAGGGAAC ACAGAGCTAAAAGGCAAATCGGAGGTGGAAGAGTGGTCAGACATGTACACTGTGACCAGTCAGCTGATGCTGAAGGTGCACAAGGAGGACGATGGGGTCC CAGTGATCTGCCAGGTGGAGCACCCTGCGGTCACTGGAAACCTGCAGACCCAGCGGTATCTAGAAGTACAGTATAAGCCTCAAGTGCACATTCAGATGAC TTATCCTCTACAAGGCTTAACCCGGGAAGGGGACGCGCTTGAGTTAACATGTGAAGCCATCGGGAAGCCCCAGCCTGTGATGGTAACTTGGGTGAGAGTC GATGATGAAATGCCTCAACACGCCGTACTGTCTGGGCCCAACCTGTTCATCAATAACCTAAACAAAACAGATAATGGTACATACCGCTGTGAAGCTTCAA ACATAGTGGGGAAAGCTCACTCGGATTATATGCTGTATGTATACGATCCCCCCACAACTATCCCTCCTCCCACAACAACCACCACCACCACCACCACCAC CACCACCACCATCCTTACCATCATCACAGACACAACGGCGACGACAGAACCAGCAGTTCACGATTCCCGAGCAGGTGAAGAAGGCTCGATCAGGGCAGTG GATCATGCCGTGATCGGTGGCGTCGTGGCGGTGGTGGTGTTCGCCATGCTGTGCTTGCTCATCATTCTGGGGCGCTATTTTGCCAGACATAAAGGTACAT ACTTCACTCATGAAGCCAAAGGAGCCGATGACGCAGCAGACGCAGACACAGCTATAATCAATGCAGAAGGAGGACAGAACAACTCCGAAGAAAAGAAAGA GTACTTCATCTAGATCAGCCTTTTTGTTTCAATGAGGTGTCCAACTGGCCCTATTTAGATGATAAAGAGACAGTGATATTGGAACTTGCGAGAAATTCGT GTGTTTTTTTATGAATGGGTGGAAAGGTGTGAGACTGGGAAGGCTTGGGATTTGCTGTGTAAAAAAAAAAAAAATGTTCTTTGGAAAGTACACTCTGCTG TTTGACACCTCTTTTTTCGTTTGTTTGTTTGTTTAATTTTTATTTCTTCCTACCAAGTCAAACTTGGATACTTGGATTTAGTTTCAGTAGATTGCAGAAA ATTCTGTGCCTTGTTTTTTGTTTGTTTGTTGCGTTCCTTTCTTTTCCCCCTTTGTGCACATTTATTTCCTCCCTCTACCCCAATTTCGGATTTTTTCCAA AATCTCCCATTTTGGAATTTGCCTGCTGGGATTCCTTAGACTCTTTTCCTTCCCTTTTCTGTTCTAGTTTTTTACTTTTGTTTATTTTTATGGTAACTGC TTTCTGTTCCAAATTCAGTTTCATAAAAGGAGAACCAGCACAGCTTAGATTTCATAGTTCAGAATTTAGTGTATCCATAATGCATTCTTCTCTGTTGTCG TAAAGATTTGGGTGAACAAACAATGAAAACTCTTTGCTGCTGCCCATGTTTCAAATACTTAGAGCAGTGAAGACTAGAAAATTAGACTGTGATTCAGAAA ATGTTCTGTTTGCTGTGGAACTACATTACTGTACAGGGTTATCTGCAAGTGAGGTGTGTCACAATGAGATTGAATTTCACTGTCTTTAATTCTGTATCTG TAGACGGCTCAGTATAGATACCCTACGCTGTCCAGAAAGGTTTGGGGCAGAAAGGACTCCTCCTTTTTCCATGCCCTAAACAGACCTGACAGGTGAGGTC TGTTCCTTTTATATAAGTGGACAAATTTTGAGTTGCCACAGGAGGGGAAGTAGGGAGGGGGGAAATACAGTTCTGCTCTGGTTGTTTCTGTTCCAAATGA TTCCATCCACCTTTCCCAATCGGCCTTACTTCTCACTAATTTGTAGGAAAAAGCAAGTTCGTCTGTTGTGCGAATGACTGAATGGGACAGAGTTGATTTT TTTTTTTTTTTCCTTTGTGCTTAGTTAGGAAGGCAGTAGGATGTGGCCTGCATGTACTGTATATTACAGATATTTGTCATGCTGGGATTTCCAACTCGAA TCTGTGTGAAACTTTCATTCCTTCAGATTTGGCTTGACAAAGGCAGGAGGTACAAAAGAAGGGCTGGTATTGTTCTCACACTGGTCTGCTGTCGCTCTCA GTTCTCGATAGGTCAGAGCAGAGGTGGAAAAACAGCATGTACGGATTTTCAGTTACTTAATCAAAACTCAAATGTGAGTGTTTTTATCTTTTTACCTTTC ATACACTAGCCTTGGCCTCTTTCCTCAGCCTTAAGAACCATCTGCCAAAAATTACTGATCCTCGCATGATGGCAGCCATAGTGCATAGCTACTAAAATCA GTGACCTTGAACATATCTTAGATGGGGAGCCTCGGGAAAAGGTAGAGGAGTCACGTTACCATTTACATGTTTTAAAGAAAGAAGTGTGGGGATTTTCACT GAAACGTCTAGGAAATCTAGAAGTAGTCCTGAAGGACAGAAACTAAACTCTTACCATATGTTTGGTAAGACTCCAGACTCCAGCTAACAGTCCCTATGGA AAGATGGCATCAAAAAAGATAGATCTATATATATATATAAATATATATTCTATTACATTTTCAGTGAGTAATTTTGGATTTTGCAAGGTGCATTTTTACT ATTGTTACATTATGTGGAAAACTTATGCTGATTTATTTAAGGGGGAAAAAGTGTCAACTCTTTGTTATTTGAAAACATGTTTATTTTTCTTGTCTTTATT TTAACCTTTGATAGAACCATTGCAATATGGGGGCCTTTTGGGAACGGACTGGTATGTAAAAGAAAATCCATTATCGAGCAGCATTTTATTTACCCCTCCC CTATCCCTAGGCACTTAACCAAGACAAAAAGCCACAATGAACATCCCTTTTTCAATGAATTTTATAATCTGCAGCTCTATTCCGAGCCCTTAGCACCCAT TCCGACCATAGTATAATCATATCAAAGGGTGAGAATCATTTAGCATGTTGTTGAAAGGTTTTTTTTCAGTTGTTCTTTTTAGAAAAAAAGAAAAACAAAA ACAAAAACAAAAAAAAAAAATCACACCATTGCTCACAGAATTGGCATCTCATTTTTGGGACCTCCCATCTTTCTGTTTTGAAAAGTGTACAGTAGTGCAG TGTTCCTGATGTAACTTTATGGCTTACAATGTTGACATGTCTCAGGTTCATGTGTTGCGATTGGTGTTTTCCGTCTCAGGTAGATTGCAAAGTGTAGGCC CCACACATTGGAAAAAATAATAATAAAACAAAGCAAAAACAGGAAATTATGGATTTTAGTTGTATATTGGTTTATGTATTTTTTCTTAAGTATACAGTGC ACTGTTTGAAATGTATTGTTGAGTATTACTTTGTACAGGTTGATCACTTTTTTTAGAGTGAAGAAAGAACAAACTTGTTTTTTGTGTTTTTTAAAGGAAT ATAAAATAATGAAGGATGTATAATTGATGCCAAATAAGCTTGTTCTTTAGTCACACCGACGTCTTATTTTTCCCTTTAGGCCAGTTCTGTTTTTAAGGTG TACATGGACAATGTTACAGTGTAAGAAACTCCATATCCATATGTTCCCATTCGCATTTTGTATTGGTTCATGTATACCATTTTTACAAAAAAAAAAAGAA AAAAAAGAAGTACTATAAAATATCTGTCTTCTTAATAAAAAAAAATTAATGTTACAAAGTGA

IGSF4.F6 (forward primer)
IGSF4.R1 (reverse primer)
Single exon skipping

Frame 0

AAECACEARSVELSKDHLVFLVLNQVMGRICLR KT*Q*SRERLRPSVAKSIRVTTL*FSY*IPTGRP FISGTSGL*RTAGFSC*IFLAVNSKYH*QTSQF LMKEDTFASSIPIPHRKVTPPSQSWSHHVI**S ISRKTLRWKVRRLKSTALLWPASQPRLSGGSKGT QS*KANRRWKSGQTCTL*PVS*C*RCTRRTMGS Q*SARWSTLRSLETCRPSGI*KYSISLKCTFR* LILYKA*PGKGTRLS*HVKPSGSPSL*W*LG*ES MMKCLNTPYCLGPTCSSIT*TKQIMVHTAVKLQ T*WGKLTRIICCMYTIPPQLSLLPQQPPPPPPP PPPPSLPSSQTQRRRQNQQFTIPEQVKKARSGQW IMP*SVASWRWWCSPCCACSSFWGAILPDIKVH TSLMKPKEPMTQQTQTQL*SMQKEDRTTPKKRK STSSRSAFLFQ*GVQLALFR**RDSDIGTCEKFV CFFMNGWKGVRLGRLGICCVKKKKMFFGKYTLL FDTSFFVCLFV*FLFLPTKSNLDTWI*FQ*IAE NSVPCFLFVCCVPFFSPFVHIYFLPLPQFRIFSK ISHFGICLLGFLRLFSFPFLF*FFTFVYFYGNC FLFQIQFHKRRTSTA*IS*FRI*CIHNAFFSVV VKIWVNKQ*KLFAAAHVSNT*SSED*KIRL*FRK CSVCCGTTLLYRVICK*GVSQ*D*ISLSLILYL *TAQYRYPTLSRKVWGRKDSSFFHALNRPDR*G LFLLYKWTNFELPQEGK*GGGKYSSALVVSVPND SIHLSQSALLLTNL*EKASSSVVRMTEWDRVDF FFFFLCA*LGRQ*DVACMYCILQIFVMLGFPTR ICVKLSFLQIWLDKGRRYKRRAGIVLTLVCCRSQ FSIGQSRGGKTACTDFQLLNQNSNVSVFIFLPF IH*PWPLSSALRTICQKLLILA*WQP*CIATKI SDLEHILDGEPREKVEESRYHLHVLKKEVWGFSL KRLGNLEVVLKDRN*TLTICLVRLQTPANSPYG KMASKKIDLYIYINIYSITFSVSNFGFCKVHFY YCYIMWKTYADLFKGEKVSTLCYLKTCLFFLSLF *PLIEPLQYGGLLGTDWYVKENPLSSSILFTPP LSLGT*PRQKATMNIPFSMNFIICSSIPSP*HP FRP*YNHIKG*ESFSMLLKGFFSVVLFRKKEKQK QKQKKKITPLLTELASHFWDLPSFCFEKCTVVQ CS*CNFMAYNVDMSQVHVLRLVFSVSGRLQSVG PTHWKK***NKAKTGNYGF*LYIGLCIFS*VYSA LFEMYC*VLLCTG*SLFLE*RKNKLVFCVF*RN IK**RMYN*CQISLFFSHTDVLFFPLGQFCF*G VHGQCYSVRNSISICSHSHFVLVHVYHFYKKKKK KKKYYKISVFLIKKN*CYKV

Frame 1

QQSVPVRPGAWSSQRTILFFWF*TR*WAESVYE RRDSDRGRGCDHQLPSQ*E*RLCDSATESQQAD HLFQGLQAFEGQQVSVAEFF*Q*TQSIIDKRLNF **RKILLPALYRSPTGKLHHHHSPGPTT*SDDR YPERHCGGR*GD*SQLHCYGQQASHDYQVVQRE HRAKRQIGGGRVVRHVHCDQSADAEGAQGGRWGP SDLPGGAPCGHWKPADPAVSRSTV*ASSAHSDD LSSTRLNPGRGRA*VNM*SHREAPACDGNLGES R**NASTRRTVWAQPVHQ*PKQNR*WYIPL*SFK HSGESSLGLYAVCIRSPHNYPSSHNNHHHHHHH HHHHPYHHHRHNGDDRTSSSRFPSR*RRLDQGS GSCRDRWRRGGGGVRHAVLAHHSGALFCQT*RYI LHS*SQRSR*RSRRRHSYNQCRRRTEQLRRKER VLHLDQPFCFNEVSNWPYLDDKETVILELARNS CVFL*MGGKV*DWEGLGFAV*KKKKCSLESTLCC LTPLFSFVCLFNFYFFLPSQTWILGFSFSRLQK ILCLVFCLFVAFLSFPPLCTFISSLYPNFGFFP KSPILEFACWDSLDSFPSLFCSSFLLLFIFMVTA FCSKFSFIKGEPAQLRFHSSEFSVSIMHSSLLS *RFG*TNNENSLLLPMFQILRAVKTRKLDCDSE NVLFAVELHYCTGLSASEVCHNEIEFHCL*FCIC RRLSIDTLRCPERFGAERTPPFSMP*TDLTGEV CSFYISGQILSCHRRGSREGGNTVLLWLFLFQM IPSTFPNRPYFSLICRKKQVRLLCE*LNGTELIF FFFSFVLS*EGSRMWPACTVYYRYLSCWDFQLE SV*NFHSFRFGLTKAGGTKEGLVLFSHWSAVAL SSR*VRAEVEKQHVRIFSYLIKTQM*VFLSFYLS YTSLGLFPQP*EPSAKNY*SSHDGSHSA*LLKS VTLNIS*MGSLGKR*RSHVTIYMF*RKKCGDFH *NV*EI*K*S*RTETKLLPYVW*DSRLQLTVPME RWHQKR*IYIYI*IYILLHFQ*VILDFARCIFT IVTLCGKLMLIYLRGKKCQLFVI*KHVYFSCLY FNL**NHCNMGAFWERTGM*KKIHYRAAFYLPLP YP*ALNQDKKPQ*TSLFQ*IL*SAALFRALSTH SDHSIIISKGENHLACC*KVFFQLFFLEKKKNK NKNKKKKSHHCSQNWHLIFGTSHLSVLKSVQ*CS VPDVTLWLTMLTCLRFMCCDWCFPSQVDCKV*A PHIGKNNNKTKQKQEIMDFSCILVYVFFLKYTV HCLKCIVEYYFVQVDHFF*SEERTNLFFVFFKGI *NNEGCIIDAK*ACSLVTPTSYFSL*ASSVFKV YMDNVTV*ETPYPYVPIRILYWFMYTIFTKKKR KKRSTIKYLSS**KKINVTK*

Frame 2

SRVCL*GQERGALKGPSCFSGFKPGDGQNLFTK DVTVIEGEVATISCQVNKSDDSVIQLLNPNRQT IYFRDFRPLKDSRFQLLNFSSSELKVSLTNVSI SDEGRYFCQLYTDPPQESYTTITVLVPPRNLMID IQKDTAVEGEEIEVNCTAMASKPATTIRWFKGN TELKGKSEVEEWSDMYTVTSQLMLKVHKEDDGV PVICQVEHPAVTGNLQTQRYLEVQYKPQVHIQMT YPLQGLTREGDALELTCEAIGKPQPVMVTWVRV DDEMPQHAVLSGPNLFINNLNKTDNGTYRCEAS NIVGKAHSDYMLYVYDPPTTIPPPTTTTTTTTTT TTTILTIITDTTATTEPAVHDSRAGEEGSIRAV DHAVIGGVVAVVVFAMLCLLIILGRYFARHKGT YFTHEAKGADDAADADTAIINAEGGQNNSEEKKE YFI*ISLFVSMRCPTGPI*MIKRQ*YWNLREIR VFFYEWVERCETGKAWDLLCKKKKNVLWKVHSA V*HLFFRLFVCLIFISSYQVKLGYLDLVSVDCRK FCALFFVCLLRSFLFPLCAHLFPPSTPISDFFQ NLPFWNLPAGIP*TLFLPFSVLVFYFCLFLW*L LSVPNSVS*KENQHSLDFIVQNLVYP*CILLCCR KDLGEQTMKTLCCCPCFKYLEQ*RLEN*TVIQK MFCLLWNYITVQGYLQVRCVTMRLNFTVFNSVS VDGSV*IPYAVQKGLGQKGLLLFPCPKQT*QVRS VPFI*VDKF*VATGGEVGRGEIQFCSGCFCSK* FHPPFPIGLTSH*FVGKSKFVCCAND*MGQS*F FFFFPLCLVRKAVGCGLHVLYITDICHAGISNSN LCETFIPSDLA*QRQEVQKKGWYCSHTGLLSLS VLDRSEQRWKNSMYGFSVT*SKLKCECFYLFTF HTLALASFLSLKNHLPKITDPRMMAAIVHSY*NQ *P*TYLRWGASGKGRGVTLPFTCFKERSVGIFT ETSRKSRSSPEGQKLNSYHMFGKTPDSS*QSLW KDGIKKDRSIYIYKYIFYYIFSE*FWILQGAFLL LLHYVENLC*FI*GGKSVNSLLFENMFIFLVFI LTFDRTIAIWGPFGNGLVCKRKSIIEQHFIYPS PIPRHLTKTKSHNEHPFFNEFYNLQLYSEPLAPI PTIV*SYQRVRII*HVVERFFFSCSF*KKRKTK TKTKKKNHTIAHRIGISFLGPPIFLF*KVYSSA VFLM*LYGLQC*HVSGSCVAIGVFRLR*IAKCRP HTLEKIIIKQSKNRKLWILVVYWFMYFFLSIQC TV*NVLLSITLYRLITFFRVKKEQTCFLCFLKE YKIMKDV*LMPNKLVL*SHRRLIFPFRPVLFLRC TWTMLQCKKLHIHMFPFAFCIGSCIPFLQKKKE KKEVL*NICLLNKKKLMLQS