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Sequences

Sequence IGSF4.aAug05

GAACGCCAGCGCCAGGGGGCGGGGTGGGGGAGGGAGCGAGGCCCTCCGAGAGCCGGGTTGGGCTCGCGGCGCTGTGATTGGTCTGCCCGGACTCCGCCTC CAGCGCATGTCATTAGCATCTCATTAGCTGTCCGCTCGGGCTCCGGAGGCAGCCAACGCCGCCAGTCTGAGGCAGGTGCCCGACATGGCGAGTGTAGTGC TGCCGAGCGGATCCCAGTGTGCGGCGGCAGCGGCGGCGGCGGCGCCTCCCGGGCTCCGGCTCCGGCTTCTGCTGTTGCTCTTCTCCGCCGCGGCACTGAT CCCCACAGGTGATGGGCAGAATCTGTTTACGAAAGACGTGACAGTGATCGAGGGAGAGGTTGCGACCATCAGTTGCCAAGTCAATAAGAGTGACGACTCT GTGATTCAGCTACTGAATCCCAACAGGCAGACCATTTATTTCAGGGACTTCAGGCCTTTGAAGGACAGCAGGTTTCAGTTGCTGAATTTTTCTAGCAGTG AACTCAAAGTATCATTGACAAACGTCTCAATTTCTGATGAAGGAAGATACTTTTGCCAGCTCTATACCGATCCCCCACAGGAAAGTTACACCACCATCAC AGTCCTGGTCCCACCACGTAATCTGATGATCGATATCCAGAAAGACACTGCGGTGGAAGGTGAGGAGATTGAAGTCAACTGCACTGCTATGGCCAGCAAG CCAGCCACGACTATCAGGTGGTTCAAAGGGAACACAGAGCTAAAAGGCAAATCGGAGGTGGAAGAGTGGTCAGACATGTACACTGTGACCAGTCAGCTGA TGCTGAAGGTGCACAAGGAGGACGATGGGGTCCCAGTGATCTGCCAGGTGGAGCACCCTGCGGTCACTGGAAACCTGCAGACCCAGCGGTATCTAGAAGT ACAGTATAAGCCTCAAGTGCACATTCAGATGACTTATCCTCTACAAGGCTTAACCCGGGAAGGGGACGCGCTTGAGTTAACATGTGAAGCCATCGGGAAG CCCCAGCCTGTGATGGTAACTTGGGTGAGAGTCGATGATGAAATGCCTCAACACGCCGTACTGTCTGGGCCCAACCTGTTCATCAATAACCTAAACAAAA CAGATAATGGTACATACCGCTGTGAAGCTTCAAACATAGTGGGGAAAGCTCACTCGGATTATATGCTGTATGTATACGATCCCCCCACAACTATCCCTCC TCCCACAACAACCACCACCACCACCACCACCACCACCACCACCATCCTTACCATCATCACAGATTCCCGAGCAGGTGAAGAAGGCTCGATCAGGGCAGTG GATCATGCCGTGATCGGTGGCGTCGTGGCGGTGGTGGTGTTCGCCATGCTGTGCTTGCTCATCATTCTGGGGCGCTATTTTGCCAGACATAAAGGTACAT ACTTCACTCATGAAGCCAAAGGAGCCGATGACGCAGCAGACGCAGACACAGCTATAATCAATGCAGAAGGAGGACAGAACAACTCCGAAGAAAAGAAAGA GTACTTCATCTAGATCAGCCTTTTTGTTTCAATGAGGTGTCCAACTGGCCCTATTTAGATGATAAAGAGACAGTGATATTGGAACTTGCGAGAAATTCGT GTGTTTTTTTATGAATGGGTGGAAAGGTGTGAGACTGGGAAGGCTTGGGATTTGCTGTGTAAAAAAAAAAAAAATGTTCTTTGGAAAGTACACTCTGCTG TTTGACACCTCTTTTTTCGTTTGTTTGTTTGTTTAATTTTTATTTCTTCCTACCAAGTCAAACTTGGATACTTGGATTTAGTTTCAGTAGATTGCAGAAA ATTCTGTGCCTTGTTTTTTGTTTGTTTGTTGCGTTCCTTTCTTTTCCCCCTTTGTGCACATTTATTTCCTCCCTCTACCCCAATTTCGGATTTTTTCCAA AATCTCCCATTTTGGAATTTGCCTGCTGGGATTCCTTAGACTCTTTTCCTTCCCTTTTCTGTTCTAGTTTTTTACTTTTGTTTATTTTTATGGTAACTGC TTTCTGTTCCAAATTCAGTTTCATAAAAGGAGAACCAGCACAGCTTAGATTTCATAGTTCAGAATTTAGTGTATCCATAATGCATTCTTCTCTGTTGTCG TAAAGATTTGGGTGAACAAACAATGAAAACTCTTTGCTGCTGCCCATGTTTCAAATACTTAGAGCAGTGAAGACTAGAAAATTAGACTGTGATTCAGAAA ATGTTCTGTTTGCTGTGGAACTACATTACTGTACAGGGTTATCTGCAAGTGAGGTGTGTCACAATGAGATTGAATTTCACTGTCTTTAATTCTGTATCTG TAGACGGCTCAGTATAGATACCCTACGCTGTCCAGAAAGGTTTGGGGCAGAAAGGACTCCTCCTTTTTCCATGCCCTAAACAGACCTGACAGGTGAGGTC TGTTCCTTTTATATAAGTGGACAAATTTTGAGTTGCCACAGGAGGGGAAGTAGGGAGGGGGGAAATACAGTTCTGCTCTGGTTGTTTCTGTTCCAAATGA TTCCATCCACCTTTCCCAATCGGCCTTACTTCTCACTAATTTGTAGGAAAAAGCAAGTTCGTCTGTTGTGCGAATGACTGAATGGGACAGAGTTGATTTT TTTTTTTTTTTCCTTTGTGCTTAGTTAGGAAGGCAGTAGGATGTGGCCTGCATGTACTGTATATTACAGATATTTGTCATGCTGGGATTTCCAACTCGAA TCTGTGTGAAACTTTCATTCCTTCAGATTTGGCTTGACAAAGGCAGGAGGTACAAAAGAAGGGCTGGTATTGTTCTCACACTGGTCTGCTGTCGCTCTCA GTTCTCGATAGGTCAGAGCAGAGGTGGAAAAACAGCATGTACGGATTTTCAGTTACTTAATCAAAACTCAAATGTGAGTGTTTTTATCTTTTTACCTTTC ATACACTAGCCTTGGCCTCTTTCCTCAGCCTTAAGAACCATCTGCCAAAAATTACTGATCCTCGCATGATGGCAGCCATAGTGCATAGCTACTAAAATCA GTGACCTTGAACATATCTTAGATGGGGAGCCTCGGGAAAAGGTAGAGGAGTCACGTTACCATTTACATGTTTTAAAGAAAGAAGTGTGGGGATTTTCACT GAAACGTCTAGGAAATCTAGAAGTAGTCCTGAAGGACAGAAACTAAACTCTTACCATATGTTTGGTAAGACTCCAGACTCCAGCTAACAGTCCCTATGGA AAGATGGCATCAAAAAAGATAGATCTATATATATATATAAATATATATTCTATTACATTTTCAGTGAGTAATTTTGGATTTTGCAAGGTGCATTTTTACT ATTGTTACATTATGTGGAAAACTTATGCTGATTTATTTAAGGGGGAAAAAGTGTCAACTCTTTGTTATTTGAAAACATGTTTATTTTTCTTGTCTTTATT TTAACCTTTGATAGAACCATTGCAATATGGGGGCCTTTTGGGAACGGACTGGTATGTAAAAGAAAATCCATTATCGAGCAGCATTTTATTTACCCCTCCC CTATCCCTAGGCACTTAACCAAGACAAAAAGCCACAATGAACATCCCTTTTTCAATGAATTTTATAATCTGCAGCTCTATTCCGAGCCCTTAGCACCCAT TCCGACCATAGTATAATCATATCAAAGGGTGAGAATCATTTAGCATGTTGTTGAAAGGTTTTTTTTCAGTTGTTCTTTTTAGAAAAAAAGAAAAACAAAA ACAAAAACAAAAAAAAAAAATCACACCATTGCTCACAGAATTGGCATCTCATTTTTGGGACCTCCCATCTTTCTGTTTTGAAAAGTGTACAGTAGTGCAG TGTTCCTGATGTAACTTTATGGCTTACAATGTTGACATGTCTCAGGTTCATGTGTTGCGATTGGTGTTTTCCGTCTCAGGTAGATTGCAAAGTGTAGGCC CCACACATTGGAAAAAATAATAATAAAACAAAGCAAAAACAGGAAATTATGGATTTTAGTTGTATATTGGTTTATGTATTTTTTCTTAAGTATACAGTGC ACTGTTTGAAATGTATTGTTGAGTATTACTTTGTACAGGTTGATCACTTTTTTTAGAGTGAAGAAAGAACAAACTTGTTTTTTGTGTTTTTTAAAGGAAT ATAAAATAATGAAGGATGTATAATTGATGCCAAATAAGCTTGTTCTTTAGTCACACCGACGTCTTATTTTTCCCTTTAGGCCAGTTCTGTTTTTAAGGTG TACATGGACAATGTTACAGTGTAAGAAACTCCATATCCATATGTTCCCATTCGCATTTTGTATTGGTTCATGTATACCATTTTTACAAAAAAAAAAAGAA AAAAAAGAAGTACTATAAAATATCTGTCTTCTTAATAAAAAAAAATTAATGTTACAAAGTGA

IGSF4.F6 (forward primer)
IGSF4.R1 (reverse primer)
Multiple exon skipping

Frame 0

ERQRQGAGWGRERGPPRAGLGSRRCDWSARTPP PAHVISISLAVRSGSGGSQRRQSEAGARHGECSA AERIPVCGGSGGGGASRAPAPASAVALLRRGTD PHR*WAESVYERRDSDRGRGCDHQLPSQ*E*RL CDSATESQQADHLFQGLQAFEGQQVSVAEFF*Q* TQSIIDKRLNF**RKILLPALYRSPTGKLHHHH SPGPTT*SDDRYPERHCGGR*GD*SQLHCYGQQ ASHDYQVVQREHRAKRQIGGGRVVRHVHCDQSAD AEGAQGGRWGPSDLPGGAPCGHWKPADPAVSRS TV*ASSAHSDDLSSTRLNPGRGRA*VNM*SHRE APACDGNLGESR**NASTRRTVWAQPVHQ*PKQN R*WYIPL*SFKHSGESSLGLYAVCIRSPHNYPS SHNNHHHHHHHHHHHPYHHHRFPSR*RRLDQGS GSCRDRWRRGGGGVRHAVLAHHSGALFCQT*RYI LHS*SQRSR*RSRRRHSYNQCRRRTEQLRRKER VLHLDQPFCFNEVSNWPYLDDKETVILELARNS CVFL*MGGKV*DWEGLGFAV*KKKKCSLESTLCC LTPLFSFVCLFNFYFFLPSQTWILGFSFSRLQK ILCLVFCLFVAFLSFPPLCTFISSLYPNFGFFP KSPILEFACWDSLDSFPSLFCSSFLLLFIFMVTA FCSKFSFIKGEPAQLRFHSSEFSVSIMHSSLLS *RFG*TNNENSLLLPMFQILRAVKTRKLDCDSE NVLFAVELHYCTGLSASEVCHNEIEFHCL*FCIC RRLSIDTLRCPERFGAERTPPFSMP*TDLTGEV CSFYISGQILSCHRRGSREGGNTVLLWLFLFQM IPSTFPNRPYFSLICRKKQVRLLCE*LNGTELIF FFFSFVLS*EGSRMWPACTVYYRYLSCWDFQLE SV*NFHSFRFGLTKAGGTKEGLVLFSHWSAVAL SSR*VRAEVEKQHVRIFSYLIKTQM*VFLSFYLS YTSLGLFPQP*EPSAKNY*SSHDGSHSA*LLKS VTLNIS*MGSLGKR*RSHVTIYMF*RKKCGDFH *NV*EI*K*S*RTETKLLPYVW*DSRLQLTVPME RWHQKR*IYIYI*IYILLHFQ*VILDFARCIFT IVTLCGKLMLIYLRGKKCQLFVI*KHVYFSCLY FNL**NHCNMGAFWERTGM*KKIHYRAAFYLPLP YP*ALNQDKKPQ*TSLFQ*IL*SAALFRALSTH SDHSIIISKGENHLACC*KVFFQLFFLEKKKNK NKNKKKKSHHCSQNWHLIFGTSHLSVLKSVQ*CS VPDVTLWLTMLTCLRFMCCDWCFPSQVDCKV*A PHIGKNNNKTKQKQEIMDFSCILVYVFFLKYTV HCLKCIVEYYFVQVDHFF*SEERTNLFFVFFKGI *NNEGCIIDAK*ACSLVTPTSYFSL*ASSVFKV YMDNVTV*ETPYPYVPIRILYWFMYTIFTKKKR KKRSTIKYLSS**KKINVTK*

Frame 1

NASARGRGGGGSEALREPGWARGAVIGLPGLRL QRMSLASH*LSARAPEAANAASLRQVPDMASVV LPSGSQCAAAAAAAAPPGLRLRLLLLLFSAAALI PTGDGQNLFTKDVTVIEGEVATISCQVNKSDDS VIQLLNPNRQTIYFRDFRPLKDSRFQLLNFSSS ELKVSLTNVSISDEGRYFCQLYTDPPQESYTTIT VLVPPRNLMIDIQKDTAVEGEEIEVNCTAMASK PATTIRWFKGNTELKGKSEVEEWSDMYTVTSQL MLKVHKEDDGVPVICQVEHPAVTGNLQTQRYLEV QYKPQVHIQMTYPLQGLTREGDALELTCEAIGK PQPVMVTWVRVDDEMPQHAVLSGPNLFINNLNK TDNGTYRCEASNIVGKAHSDYMLYVYDPPTTIPP PTTTTTTTTTTTTTILTIITDSRAGEEGSIRAV DHAVIGGVVAVVVFAMLCLLIILGRYFARHKGT YFTHEAKGADDAADADTAIINAEGGQNNSEEKKE YFI*ISLFVSMRCPTGPI*MIKRQ*YWNLREIR VFFYEWVERCETGKAWDLLCKKKKNVLWKVHSA V*HLFFRLFVCLIFISSYQVKLGYLDLVSVDCRK FCALFFVCLLRSFLFPLCAHLFPPSTPISDFFQ NLPFWNLPAGIP*TLFLPFSVLVFYFCLFLW*L LSVPNSVS*KENQHSLDFIVQNLVYP*CILLCCR KDLGEQTMKTLCCCPCFKYLEQ*RLEN*TVIQK MFCLLWNYITVQGYLQVRCVTMRLNFTVFNSVS VDGSV*IPYAVQKGLGQKGLLLFPCPKQT*QVRS VPFI*VDKF*VATGGEVGRGEIQFCSGCFCSK* FHPPFPIGLTSH*FVGKSKFVCCAND*MGQS*F FFFFPLCLVRKAVGCGLHVLYITDICHAGISNSN LCETFIPSDLA*QRQEVQKKGWYCSHTGLLSLS VLDRSEQRWKNSMYGFSVT*SKLKCECFYLFTF HTLALASFLSLKNHLPKITDPRMMAAIVHSY*NQ *P*TYLRWGASGKGRGVTLPFTCFKERSVGIFT ETSRKSRSSPEGQKLNSYHMFGKTPDSS*QSLW KDGIKKDRSIYIYKYIFYYIFSE*FWILQGAFLL LLHYVENLC*FI*GGKSVNSLLFENMFIFLVFI LTFDRTIAIWGPFGNGLVCKRKSIIEQHFIYPS PIPRHLTKTKSHNEHPFFNEFYNLQLYSEPLAPI PTIV*SYQRVRII*HVVERFFFSCSF*KKRKTK TKTKKKNHTIAHRIGISFLGPPIFLF*KVYSSA VFLM*LYGLQC*HVSGSCVAIGVFRLR*IAKCRP HTLEKIIIKQSKNRKLWILVVYWFMYFFLSIQC TV*NVLLSITLYRLITFFRVKKEQTCFLCFLKE YKIMKDV*LMPNKLVL*SHRRLIFPFRPVLFLRC TWTMLQCKKLHIHMFPFAFCIGSCIPFLQKKKE KKEVL*NICLLNKKKLMLQS

Frame 2

TPAPGGGVGEGARPSESRVGLAAL*LVCPDSAS SACH*HLISCPLGLRRQPTPPV*GRCPTWRV*C CRADPSVRRQRRRRRLPGSGSGFCCCSSPPRH* SPQVMGRICLRKT*Q*SRERLRPSVAKSIRVTTL *FSY*IPTGRPFISGTSGL*RTAGFSC*IFLAV NSKYH*QTSQFLMKEDTFASSIPIPHRKVTPPS QSWSHHVI**SISRKTLRWKVRRLKSTALLWPAS QPRLSGGSKGTQS*KANRRWKSGQTCTL*PVS* C*RCTRRTMGSQ*SARWSTLRSLETCRPSGI*K YSISLKCTFR*LILYKA*PGKGTRLS*HVKPSGS PSL*W*LG*ESMMKCLNTPYCLGPTCSSIT*TK QIMVHTAVKLQT*WGKLTRIICCMYTIPPQLSL LPQQPPPPPPPPPPPSLPSSQIPEQVKKARSGQW IMP*SVASWRWWCSPCCACSSFWGAILPDIKVH TSLMKPKEPMTQQTQTQL*SMQKEDRTTPKKRK STSSRSAFLFQ*GVQLALFR**RDSDIGTCEKFV CFFMNGWKGVRLGRLGICCVKKKKMFFGKYTLL FDTSFFVCLFV*FLFLPTKSNLDTWI*FQ*IAE NSVPCFLFVCCVPFFSPFVHIYFLPLPQFRIFSK ISHFGICLLGFLRLFSFPFLF*FFTFVYFYGNC FLFQIQFHKRRTSTA*IS*FRI*CIHNAFFSVV VKIWVNKQ*KLFAAAHVSNT*SSED*KIRL*FRK CSVCCGTTLLYRVICK*GVSQ*D*ISLSLILYL *TAQYRYPTLSRKVWGRKDSSFFHALNRPDR*G LFLLYKWTNFELPQEGK*GGGKYSSALVVSVPND SIHLSQSALLLTNL*EKASSSVVRMTEWDRVDF FFFFLCA*LGRQ*DVACMYCILQIFVMLGFPTR ICVKLSFLQIWLDKGRRYKRRAGIVLTLVCCRSQ FSIGQSRGGKTACTDFQLLNQNSNVSVFIFLPF IH*PWPLSSALRTICQKLLILA*WQP*CIATKI SDLEHILDGEPREKVEESRYHLHVLKKEVWGFSL KRLGNLEVVLKDRN*TLTICLVRLQTPANSPYG KMASKKIDLYIYINIYSITFSVSNFGFCKVHFY YCYIMWKTYADLFKGEKVSTLCYLKTCLFFLSLF *PLIEPLQYGGLLGTDWYVKENPLSSSILFTPP LSLGT*PRQKATMNIPFSMNFIICSSIPSP*HP FRP*YNHIKG*ESFSMLLKGFFSVVLFRKKEKQK QKQKKKITPLLTELASHFWDLPSFCFEKCTVVQ CS*CNFMAYNVDMSQVHVLRLVFSVSGRLQSVG PTHWKK***NKAKTGNYGF*LYIGLCIFS*VYSA LFEMYC*VLLCTG*SLFLE*RKNKLVFCVF*RN IK**RMYN*CQISLFFSHTDVLFFPLGQFCF*G VHGQCYSVRNSISICSHSHFVLVHVYHFYKKKKK KKKYYKISVFLIKKN*CYKV

Sequence IGSF4.oAug05

AGAGCAGCACCACCTCCTCCTCCTCCTTCCTGCCATCTGTAAAAGCTGAAACGAAATGACAACCATTGTACCCTCTTGGTACTGAGTTGATTTAACCTCA CCTTTGAAATCTGGTAGGGAAGAGGGGTGTCGTATGTTTTGATTTTTCCTCCCCTCTCTTCACATCTCCTCATCCCTAATTCACTTCATTAATGAAAATT CTCTTTTCTTCTTTTCTCAGCTCTTCCAACTAAGGCTTTGGGATATAATTACAAGACATAAATCTTGCAGGGAAAGAACTTTTGGTTTTAAAGGGGGAAG CCATCAAACAAACATCTCAATGTCCTCATGCCTTTGTTTTGTCATTACTGGTCATTGTTGTGTTCAGCATTGTGGGTTTGATTTTCCTTTTTTTTTCTAT CCAGATCCCCCCACAACTATCCCTCCTCCCACAACAACCACCACCACCACCACCACCACCACCACCACCATCCTTACCATCATCACAGACACAACGGCGA CGACAGAACCAGCAGTTCACGGCCTTACTCAGTTGCCCAATTCCGCAGAAGAACTGGACAGTGAGGACCTCTCAGATTCCCGAGCAGGTGAAGAAGGCTC GATCAGGGCAGTGGATCATGCCGTGATCGGTGGCGTCGTGGCGGTGGTGGTGTTCGCCATGCTGTGCTTGCTCATCATTCTGGGGCGCTATTTTGCCAGA CATAAAGGCCTGTTTTCTCTAACCTCCTCTCCCAGAATAAAGTGATGAGGGCAGCTATAGTGTTGCAATTAGGAACATGAATCTTTTATCGTTTGGACAA GGAAGGGAAAATGCCATTACTGTCAACTGAAATGTCCTACTTGGGTTTTTTGCTCCTACTTCCCTGCCAAAGCCCATTGCTGCTGCTGAAATTGTATAAC ATTCACAGCTGTTCTTGATGGCTTGATTCTTAACACAGCCAGAAATGTAGCCCGGGAGATGCTTAAGACAGTTTATGGAAAATAATAGATTTCCTTGCAG AGACACCAATGCATCGAGAAGGGCCTTGAGTCACGTTAGCCCCAGCCAAGATCACCAATTTTCTCTCTATCTGGCTTCAGTTACAAAGGCTATGCCTT

IGSF4.F6 (forward primer)
IGSF4.R1 (reverse primer)
Multiple exon skipping

Frame 0

RAAPPPPPPSCHL*KLKRNDNHCTLLVLS*FNL TFEIW*GRGVSYVLIFPPLSSHLLIPNSLH**KF SFLLFSALPTKALGYNYKT*ILQGKNFWF*RGK PSNKHLNVLMPLFCHYWSLLCSALWV*FSFFFL SRSPHNYPSSHNNHHHHHHHHHHHPYHHHRHNGD DRTSSSRPYSVAQFRRRTGQ*GPLRFPSR*RRL DQGSGSCRDRWRRGGGGVRHAVLAHHSGALFCQ T*RPVFSNLLSQNKVMRAAIVLQLGT*IFYRLDK EGKMPLLSTEMSYLGFLLLLPCQSPLLLLKLYN IHSCS*WLDS*HSQKCSPGDA*DSLWKIIDFLA ETPMHREGP*VTLAPAKITNFLSIWLQLQRLCL

Frame 1

EQHHLLLLLPAICKS*NEMTTIVPSWY*VDLTS PLKSGREEGCRMF*FFLPSLHISSSLIHFINEN SLFFFSQLFQLRLWDIITRHKSCRERTFGFKGGS HQTNISMSSCLCFVITGHCCVQHCGFDFPFFFY PDPPTTIPPPTTTTTTTTTTTTTILTIITDTTA TTEPAVHGLTQLPNSAEELDSEDLSDSRAGEEGS IRAVDHAVIGGVVAVVVFAMLCLLIILGRYFAR HKGLFSLTSSPRIK**GQL*CCN*EHESFIVWT RKGKCHYCQLKCPTWVFCSYFPAKAHCCC*NCIT FTAVLDGLILNTARNVAREMLKTVYGK**ISLQ RHQCIEKGLESR*PQPRSPIFSLSGFSYKGYA

Frame 2

SSTTSSSSFLPSVKAETK*QPLYPLGTELI*PH L*NLVGKRGVVCFDFSSPLFTSPHP*FTSLMKI LFSSFLSSSN*GFGI*LQDINLAGKELLVLKGE AIKQTSQCPHAFVLSLLVIVVFSIVGLIFLFFSI QIPPQLSLLPQQPPPPPPPPPPPSLPSSQTQRR RQNQQFTALLSCPIPQKNWTVRTSQIPEQVKKA RSGQWIMP*SVASWRWWCSPCCACSSFWGAILPD IKACFL*PPLPE*SDEGSYSVAIRNMNLLSFGQ GRENAITVN*NVLLGFFAPTSLPKPIAAAEIV* HSQLFLMA*FLTQPEM*PGRCLRQFMENNRFPCR DTNASRRALSHVSPSQDHQFSLYLASVTKAMP

Sequence IGSF4.gAug05

GCCAGGATTACCAACTAATTAGAAGGGTGTTACTGAAGGCATCGGATGGGAGAGAGTAAGTGCTGGTACTGTGGTTTTGAGGCAGTGTGTGAGTCTGATT TTGCCTGATGATATCATTTGCTAAACATTATACTTTGTGTTTTTGTCACACAGATAAGCCTCAAGTGCACATTCAGATGACTTATCCTCTACAAGGCTTA ACCCGGGAAGGGGACGCGCTTGAGTTAACATGTGAAGCCATCGGGAAGCCCCAGCCTGTGATGGTAACTTGGGTGAGAGTCGATGATGAAATGCCTCAAC ACGCCGTACTGTCTGGGCCCAACCTGTTCATCAATAACCTAAACAAAACAGATAATGGTACATACCGCTGTGAAGCTTCAAACATAGTGGGGAAAGCTCA CTCGGATTATATGCTGTATGTATACGATCCCCCCACAACTATCCCTCCTCCCACAACAACCACCACCACCACCACCACCACCACCACCACCATCCTTACC ATCATCACAGACACAACGGCGACGACAGAACCAGCAGTTCACGGCCTTACTCAGTTGCCCAATTCCGCAGAAGAACTGGACAGTGAGGACCTCTCAGATT CCCGAGCAGGTGAAGAAGGCTCGATCAGGGCAGTGGATCATGCCGTGATCGGTGGCGTCGTGGCGGTGGTGGTGTTCGCCATGCTGTGCTTGCTCATCAT TCTGGGGCGCTATTTTGCCAGACATAAAGGTACATACTTCACTCATGAAGCCAAAGGAGCCGATGACGCAGCAGACGCAGACACAGCTATAATCAATGCA GAAGGAGGACAGAACAACTCCGAAGAAAAGAAAGAGTACTTCATCTAGATCAGCCTTTTTGTTTCAATGAGGTGTCCAACTGGCCCTATTTAGATGATAA AGAGACAGTGATATTGGAACTTGCGAGAAATTCGTGTGTTTTTTTATGAATGGGTGGAAAGGTGTGAGACTGGGAAGGCTTGGGATTTGCTGTGTAAAAA AAAAAAAAATGTTCTTTGGAAAGTACACTCTGCTGTTTGACACCTCTTTTTTCGTTTGTTTGTTTGTTTAATTTTTATTTCTTCCTACCAAGTCAAACTT GGATACTTGGATTTAGTTTCAGTAGATTGCAGAAAATTCTGTGCCTTGTTTTTTGTTTGTTTGTTGCGTTCCTTTCTTTTCCCCCTTTGTGCACATTTAT TTCCTCCCTCTACCCCAATTTCGGATTTTTTCCAAAATCTCCCATTTTGGAATTTGCCTGCTGGGATTCCTTAGACTCTTTTCCTTCCCTTTTCTGTTCT AGTTTTTTACTTTTGTTTATTTTTATGGTAACTGCTTTCTGTTCCAAATTCAGTTTCATAAAAGGAGAACCAGCACAGCTTAGATTTCATAGTTCAGAAT TTAGTGTATCCATAATGCATTCTTCTCTGTTGTCGTAAAGATTTGGGTGAACAAACAATGAAAACTCTTTGCTGCTGCCCATGTTTCAAATACTTAGAGC AGTGAAGACTAGAAAATTAGACTGTGATTCAGAAAATGTTCTGTTTGCTGTGGAACTACATTACTGTACAGGGTTATCTGCAAGTGAGGTGTGTCACAAT GAGATTGAATTTCACTGTCTTTAATTCTGTATCTGTAGACGGCTCAGTATAGATACCCTACGCTGTCCAGAAAGGTTTGGGGCAGAAAGGACTCCTCCTT TTTCCATGCCCTAAACAGACCTGACAGGTGAGGTCTGTTCCTTTTATATAAGTGGACAAATTTTGAGTTGCCACAGGAGGGGAAGTAGGGAGGGGGGAAA TACAGTTCTGCTCTGGTTGTTTCTGTTCCAAATGATTCCATCCACCTTTCCCAATCGGCCTTACTTCTCACTAATTTGTAGGAAAAAGCAAGTTCGTCTG TTGTGCGAATGACTGAATGGGACAGAGTTGATTTTTTTTTTTTTTTCCTTTGTGCTTAGTTAGGAAGGCAGTAGGATGTGGCCTGCATGTACTGTATATT ACAGATATTTGTCATGCTGGGATTTCCAACTCGAATCTGTGTGAAACTTTCATTCCTTCAGATTTGGCTTGACAAAGGCAGGAGGTACAAAAGAAGGGCT GGTATTGTTCTCACACTGGTCTGCTGTCGCTCTCAGTTCTCGATAGGTCAGAGCAGAGGTGGAAAAACAGCATGTACGGATTTTCAGTTACTTAATCAAA ACTCAAATGTGAGTGTTTTTATCTTTTTACCTTTCATACACTAGCCTTGGCCTCTTTCCTCAGCCTTAAGAACCATCTGCCAAAAATTACTGATCCTCGC ATGATGGCAGCCATAGTGCATAGCTACTAAAATCAGTGACCTTGAACATATCTTAGATGGGGAGCCTCGGGAAAAGGTAGAGGAGTCACGTTACCATTTA CATGTTTTAAAGAAAGAAGTGTGGGGATTTTCACTGAAACGTCTAGGAAATCTAGAAGTAGTCCTGAAGGACAGAAACTAAACTCTTACCATATGTTTGG TAAGACTCCAGACTCCAGCTAACAGTCCCTATGGAAAGATGGCATCAAAAAAGATAGATCTATATATATATATAAATATATATTCTATTACATTTTCAGT GAGTAATTTTGGATTTTGCAAGGTGCATTTTTACTATTGTTACATTATGTGGAAAACTTATGCTGATTTATTTAAGGGGGAAAAAGTGTCAACTCTTTGT TATTTGAAAACATGTTTATTTTTCTTGTCTTTATTTTAACCTTTGATAGAACCATTGCAATATGGGGGCCTTTTGGGAACGGACTGGTATGTAAAAGAAA ATCCATTATCGAGCAGCATTTTATTTACCCCTCCCCTATCCCTAGGCACTTAACCAAGACAAAAAGCCACAATGAACATCCCTTTTTCAATGAATTTTAT AATCTGCAGCTCTATTCCGAGCCCTTAGCACCCATTCCGACCATAGTATAATCATATCAAAGGGTGAGAATCATTTAGCATGTTGTTGAAAGGTTTTTTT TCAGTTGTTCTTTTTAGAAAAAAAGAAAAACAAAAACAAAAACAAAAAAAAAAAATCACACCATTGCTCACAGAATTGGCATCTCATTTTTGGGACCTCC CATCTTTCTGTTTTGAAAAGTGTACAGTAGTGCAGTGTTCCTGATGTAACTTTATGGCTTACAATGTTGACATGTCTCAGGTTCATGTGTTGCGATTGGT GTTTTCCGTCTCAGGTAGATTGCAAAGTGTAGGCCCCACACATTGGAAAAAATAATAATAAAACAAAGCAAAAACAGGAAATTATGGATTTTAGTTGTAT ATTGGTTTATGTATTTTTTCTTAAGTATACAGTGCACTGTTTGAAATGTATTGTTGAGTATTACTTTGTACAGGTTGATCACTTTTTTTAGAGTGAAGAA AGAACAAACTTGTTTTTTGTGTTTTTTAAAGGAATATAAAATAATGAAGGATGTATAATTGATGCCAAATAAGCTTGTTCTTTAGTCACACCGACGTCTT ATTTTTCCCTTTAGGCCAGTTCTGTTTTTAAGGTGTACATGGACAATGTTACAGTGTAAGAAACTCCATATCCATATGTTCCCATTCGCATTTTGTATTG GTTCATGTATACCATTTTTACAAAAAAAAAAAGAAAAAAAAGAAGTACTATAAAATATCTGTCTTCTTAATAAAAAAAAATTAATGTTACAAAGTGA

IGSF4.F6 (forward primer)
IGSF4.R1 (reverse primer)
Multiple exon skipping

Frame 0

ARITN*LEGCY*RHRMGESKCWYCGFEAVCESD FA**YHLLNIILCVFVTQISLKCTFR*LILYKA* PGKGTRLS*HVKPSGSPSL*W*LG*ESMMKCLN TPYCLGPTCSSIT*TKQIMVHTAVKLQT*WGKL TRIICCMYTIPPQLSLLPQQPPPPPPPPPPPSLP SSQTQRRRQNQQFTALLSCPIPQKNWTVRTSQI PEQVKKARSGQWIMP*SVASWRWWCSPCCACSS FWGAILPDIKVHTSLMKPKEPMTQQTQTQL*SMQ KEDRTTPKKRKSTSSRSAFLFQ*GVQLALFR** RDSDIGTCEKFVCFFMNGWKGVRLGRLGICCVK KKKMFFGKYTLLFDTSFFVCLFV*FLFLPTKSNL DTWI*FQ*IAENSVPCFLFVCCVPFFSPFVHIY FLPLPQFRIFSKISHFGICLLGFLRLFSFPFLF *FFTFVYFYGNCFLFQIQFHKRRTSTA*IS*FRI *CIHNAFFSVVVKIWVNKQ*KLFAAAHVSNT*S SED*KIRL*FRKCSVCCGTTLLYRVICK*GVSQ *D*ISLSLILYL*TAQYRYPTLSRKVWGRKDSSF FHALNRPDR*GLFLLYKWTNFELPQEGK*GGGK YSSALVVSVPNDSIHLSQSALLLTNL*EKASSS VVRMTEWDRVDFFFFFLCA*LGRQ*DVACMYCIL QIFVMLGFPTRICVKLSFLQIWLDKGRRYKRRA GIVLTLVCCRSQFSIGQSRGGKTACTDFQLLNQ NSNVSVFIFLPFIH*PWPLSSALRTICQKLLILA *WQP*CIATKISDLEHILDGEPREKVEESRYHL HVLKKEVWGFSLKRLGNLEVVLKDRN*TLTICL VRLQTPANSPYGKMASKKIDLYIYINIYSITFSV SNFGFCKVHFYYCYIMWKTYADLFKGEKVSTLC YLKTCLFFLSLF*PLIEPLQYGGLLGTDWYVKE NPLSSSILFTPPLSLGT*PRQKATMNIPFSMNFI ICSSIPSP*HPFRP*YNHIKG*ESFSMLLKGFF SVVLFRKKEKQKQKQKKKITPLLTELASHFWDL PSFCFEKCTVVQCS*CNFMAYNVDMSQVHVLRLV FSVSGRLQSVGPTHWKK***NKAKTGNYGF*LY IGLCIFS*VYSALFEMYC*VLLCTG*SLFLE*R KNKLVFCVF*RNIK**RMYN*CQISLFFSHTDVL FFPLGQFCF*GVHGQCYSVRNSISICSHSHFVL VHVYHFYKKKKKKKKYYKISVFLIKKN*CYKV

Frame 1

PGLPTN*KGVTEGIGWERVSAGTVVLRQCVSLI LPDDIIC*TLYFVFLSHR*ASSAHSDDLSSTRL NPGRGRA*VNM*SHREAPACDGNLGESR**NAST RRTVWAQPVHQ*PKQNR*WYIPL*SFKHSGESS LGLYAVCIRSPHNYPSSHNNHHHHHHHHHHHPY HHHRHNGDDRTSSSRPYSVAQFRRRTGQ*GPLRF PSR*RRLDQGSGSCRDRWRRGGGGVRHAVLAHH SGALFCQT*RYILHS*SQRSR*RSRRRHSYNQC RRRTEQLRRKERVLHLDQPFCFNEVSNWPYLDDK ETVILELARNSCVFL*MGGKV*DWEGLGFAV*K KKKCSLESTLCCLTPLFSFVCLFNFYFFLPSQT WILGFSFSRLQKILCLVFCLFVAFLSFPPLCTFI SSLYPNFGFFPKSPILEFACWDSLDSFPSLFCS SFLLLFIFMVTAFCSKFSFIKGEPAQLRFHSSE FSVSIMHSSLLS*RFG*TNNENSLLLPMFQILRA VKTRKLDCDSENVLFAVELHYCTGLSASEVCHN EIEFHCL*FCICRRLSIDTLRCPERFGAERTPP FSMP*TDLTGEVCSFYISGQILSCHRRGSREGGN TVLLWLFLFQMIPSTFPNRPYFSLICRKKQVRL LCE*LNGTELIFFFFSFVLS*EGSRMWPACTVY YRYLSCWDFQLESV*NFHSFRFGLTKAGGTKEGL VLFSHWSAVALSSR*VRAEVEKQHVRIFSYLIK TQM*VFLSFYLSYTSLGLFPQP*EPSAKNY*SS HDGSHSA*LLKSVTLNIS*MGSLGKR*RSHVTIY MF*RKKCGDFH*NV*EI*K*S*RTETKLLPYVW *DSRLQLTVPMERWHQKR*IYIYI*IYILLHFQ *VILDFARCIFTIVTLCGKLMLIYLRGKKCQLFV I*KHVYFSCLYFNL**NHCNMGAFWERTGM*KK IHYRAAFYLPLPYP*ALNQDKKPQ*TSLFQ*IL *SAALFRALSTHSDHSIIISKGENHLACC*KVFF QLFFLEKKKNKNKNKKKKSHHCSQNWHLIFGTS HLSVLKSVQ*CSVPDVTLWLTMLTCLRFMCCDW CFPSQVDCKV*APHIGKNNNKTKQKQEIMDFSCI LVYVFFLKYTVHCLKCIVEYYFVQVDHFF*SEE RTNLFFVFFKGI*NNEGCIIDAK*ACSLVTPTS YFSL*ASSVFKVYMDNVTV*ETPYPYVPIRILYW FMYTIFTKKKRKKRSTIKYLSS**KKINVTK*

Frame 2

QDYQLIRRVLLKASDGRE*VLVLWF*GSV*V*F CLMISFAKHYTLCFCHTDKPQVHIQMTYPLQGL TREGDALELTCEAIGKPQPVMVTWVRVDDEMPQ HAVLSGPNLFINNLNKTDNGTYRCEASNIVGKAH SDYMLYVYDPPTTIPPPTTTTTTTTTTTTTILT IITDTTATTEPAVHGLTQLPNSAEELDSEDLSD SRAGEEGSIRAVDHAVIGGVVAVVVFAMLCLLII LGRYFARHKGTYFTHEAKGADDAADADTAIINA EGGQNNSEEKKEYFI*ISLFVSMRCPTGPI*MI KRQ*YWNLREIRVFFYEWVERCETGKAWDLLCKK KKNVLWKVHSAV*HLFFRLFVCLIFISSYQVKL GYLDLVSVDCRKFCALFFVCLLRSFLFPLCAHL FPPSTPISDFFQNLPFWNLPAGIP*TLFLPFSVL VFYFCLFLW*LLSVPNSVS*KENQHSLDFIVQN LVYP*CILLCCRKDLGEQTMKTLCCCPCFKYLE Q*RLEN*TVIQKMFCLLWNYITVQGYLQVRCVTM RLNFTVFNSVSVDGSV*IPYAVQKGLGQKGLLL FPCPKQT*QVRSVPFI*VDKF*VATGGEVGRGE IQFCSGCFCSK*FHPPFPIGLTSH*FVGKSKFVC CAND*MGQS*FFFFFPLCLVRKAVGCGLHVLYI TDICHAGISNSNLCETFIPSDLA*QRQEVQKKG WYCSHTGLLSLSVLDRSEQRWKNSMYGFSVT*SK LKCECFYLFTFHTLALASFLSLKNHLPKITDPR MMAAIVHSY*NQ*P*TYLRWGASGKGRGVTLPF TCFKERSVGIFTETSRKSRSSPEGQKLNSYHMFG KTPDSS*QSLWKDGIKKDRSIYIYKYIFYYIFS E*FWILQGAFLLLLHYVENLC*FI*GGKSVNSL LFENMFIFLVFILTFDRTIAIWGPFGNGLVCKRK SIIEQHFIYPSPIPRHLTKTKSHNEHPFFNEFY NLQLYSEPLAPIPTIV*SYQRVRII*HVVERFF FSCSF*KKRKTKTKTKKKNHTIAHRIGISFLGPP IFLF*KVYSSAVFLM*LYGLQC*HVSGSCVAIG VFRLR*IAKCRPHTLEKIIIKQSKNRKLWILVV YWFMYFFLSIQCTV*NVLLSITLYRLITFFRVKK EQTCFLCFLKEYKIMKDV*LMPNKLVL*SHRRL IFPFRPVLFLRCTWTMLQCKKLHIHMFPFAFCI GSCIPFLQKKKEKKEVL*NICLLNKKKLMLQS