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Sequences

Sequence FGFR2.uAug05

AGGAAGTGTGCAGATGGGATTAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTG GTCGTGGTCACCATGGCAACCTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGATGCCATCTCATCCGGAGATG ATGAGGATGACACCGATGGTGCGGAAGATTTTGTCAGTGAGAACAGTAACAACAAGAGTAAGTAACTGCCCGGCTCCGATGGTCCCCGAGAGAGGAGCAT GGAGGGAAGTTCTGCCTGTCACCTGTCTTCTTGTCGACTCTTCTGCGCCATGCTGTGTCCCGCGGCCCTTGCCTTTCCCCGCTGTGTCTACTTTCCTGAC TTTCAAACCTGAGAATAAACCAGTGTTGCTGCACAGCC

FGFR2.F1 (forward primer)
FGFR2.R1 (reverse primer)
Single exon skipping

Frame 0

RKCADGINVHMEIWKRTGDWYRNHGQLGSFHLP GRGHHGNLVPGPALLQFS*GYHIRARRCHLIRR* *G*HRWCGRFCQ*EQ*QQE*VTARLRWSPREEH GGKFCLSPVFLSTLLRHAVSRGPCLSPLCLLS* LSNLRINQCCCTA

Frame 1

GSVQMGLTSTWRYGRGPGIGTVTMVSWGRFICL VVVTMATLSLARPSFSLVEDTTLEPEDAISSGD DEDDTDGAEDFVSENSNNKSK*LPGSDGPRERSM EGSSACHLSSCRLFCAMLCPAALAFPRCVYFPD FQT*E*TSVAAQ

Frame 2

EVCRWD*RPHGDMEEDRGLVP*PWSAGVVSSAW SWSPWQPCPWPGPPSV*LRIPH*SQKMPSHPEM MRMTPMVRKILSVRTVTTRVSNCPAPMVPERGA WREVLPVTCLLVDSSAPCCVPRPLPFPAVSTFLT FKPENKPVLLHS

Sequence FGFR2.mAug05

FGFR2.F1 (forward primer)
FGFR2.R1 (reverse primer)
Single exon skipping

Frame 0

Frame 1

Frame 2

Sequence FGFR2.hAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGT GCGGAAGATTTTGTCAGTGAGAACAGTAACAACAAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCA ACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGAACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGG CTACAAGGTACGAAACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCC ATCAATCACACGTACCACCTGGATGTTGTGGAGCGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAG ACGTAGAGTTTGTCTGCAAGGTTTACAGTGATGCCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCT GCCCTACCTCAAGGTTCTCAAGCACTCGGGGATAAATAGTTCCAATGCAGAAGTGCTGGCTCTGTTCAATGTGACCGAGGCGGATGCTGGGGAATATATA TGTAAGGTCTCCAATTATATAGGGCAGGCCAACCAGTCTGCCTGGCTCACTGTCCTGCCAAAACAGCAAGCGCCTGGAAGAGAAAAGGAGATTACAGCTT CCCCAGACTACCTGGAGATAGCCATTTACTGCATAGGGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAA GAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAAGCTGACCAAACGTATCCCCCTGCGGAGACAGGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCC AACACCCCGCTGGTGAGGATAACAACACGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAAT GGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAA GCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATG ATTGGGAAACACAAGAATATCATAAATCTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAG AATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCAC CTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATG AAAATAGCAGACTTTGGACTCGCCAGAGATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAG CCCTGTTTGATAGAGTATACACTCATCAGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGAT TCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGG CATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTCAGCC AACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAGACCCCATGCCTTACGAACC ATGCCTTCCTCAGTATCCACACATAAACGGCAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCACTGGGAACCTAGCTACACT GAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAGTAATCAGCATATGTGTAAAGAT TTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCATGTAACCCCTCTCACCTGCCGTG CGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAAGATTTATGTCAGCACACACTTA CAGAGCACAAATGCAGTATATAGGTGCTGGATGTATGTAAATATATTCAAATTATGTATAAATATATATTATATATTTACAAGGAGTTATTTTTTGTATT GATTTTAAATGGATGTCCCAATGCACCTAGAAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTTACACATAATTTCTTAATTTTCA CCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATACAAAACAATTAATCATTTATAGT TTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTTATCAGATCCTTTGAAAAGAGAA TATTTACAATATATGACTAATTTGGGGAAAATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCTTAGACCTCCTAAATGCCCC ATATTAAAAGAACTCATTCATAGGAAGGTGTTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAACTGGACTTCCCAAGATAAATGGT ACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCTCTGGCAGCTGGCCTTCTGC TTCTGAGTTGCACATTAATCAGATTAGCCTGTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGGAGACGCCTGTTAGGATCTT CAAGTCCCATCATAGAAAATTGAAACACAGAGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGATTGCTTTCATCTAATTCTGGCAG GACCTCACCAAAAGATCCAGCCTCATACCTACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTTTGCTTTGGAAACACCCACT CACTTTGCAATAGCCGTGCAAGATGAATGCAGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATAAAACCTGTTAATTTTTATACTG ACAATAAAAATGTTTCTACAGATATTAATGTTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F1 (forward primer)
FGFR2.R1 (reverse primer)
Single exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRCHLIRR**G*HRW CGRFCQ*EQ*QQESTILDQHRKDGKAAPCCACGQ HCQVSLPSRGEPNANHAVAEKREGV*AGASHWR LQGTKPALEPHYGKCGPI*QGKLYLCSGE*IRV HQSHVPPGCCGAIASPAHPPSRTAGKCLHSGRRR RRVCLQGLQ*CPAPHPVDQARGKERQ*IRARRA ALPQGSQALGDK*FQCRSAGSVQCDRGGCWGIY M*GLQLYRAGQPVCLAHCPAKTASAWKRKGDYSF PRLPGDSHLLHRGLLNRLYGGNSHPVPNEEHDQ EARLQQPAGCAQADQTYPPAETGFG*VQLLHEL QHPAGEDNNTPLFNGRHPHAGRGLRV*TSRGPKM GVSKR*ADTGQAPGRRLLWASGHGGSSGN*QRQ AQGGGHRGREDVER*CHRERPF*SGVRDGDDED DWETQEYHKSSWSLHTGWASLCHS*VCL*RQPPR IPPSPEATRDGVLL*H*PCS*GADDLQGLGVMH LPAGQRHGVLGFPKMYSSRFSSQKCFGNRKQCD ENSRLWTRQRYQQYRLLQKDHQWAASSQVDGSRS PV**SIHSSE*CLVLRGVNVGDLHFRGLALPRD SRGGTF*AAEGRTQNG*ASQLHQRTVHDDEGLL ACSALPETNVQAVGRRLGSNSHSHNQ*GILGPQP TSRTVFT*LP*HKKFLFFRR*FCFFSRPHALRT MPSSVSTHKRQC*NMNDCVCLSPNRTALGT*LH *AGRPCLPELVVSTCIYGSEE*IIGKVISICVKI YTVENL*SSPGGEEGFWSSGLPQATM*PLSPAV RTGCGPVGLKVDVRSAFLVNFVIIGEDLCQHTL TEHKCSI*VLDVCKYIQIMYKYILYIYKELFFVL ILNGCPNAPRKLVSLFLIAIC*MLFLHIIS*FS PSRGGKILLLSGKMV*R*FINKLVIYKTINHL* FFL*FKWHFYAGSTAD*LIYCLDLTSYQIL*KEN IYNI*LIWGK*SFDLFVFKCCCQTIVLRPPKCP ILKELIHRKVFHFGVQPCHYVNATSNWTSQDKW YQRPLKRCLNPFLEDRP*LK**QNVLLSGSWPSA SELHINQISLYSLQ*ILIMASRLFGVGDAC*DL QVPS*KIETQSCSADSFGDTSIFLRDCFHLILA GPHQKIQPHTYIRQNIAVVPSVLKYCVLLWKHPL TLQ*PCKMNADYTDLMCYKIGESI**NLLIFIL TIKMFLQILMLTRQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEDAISSGDDEDDTDG AEDFVSENSNNKRAPYWTNTEKMEKRLHAVPAA NTVKFRCPAGGNPMPTMRWLKNGKEFKQEHRIGG YKVRNQHWSLIMESVVPSDKGNYTCVVENEYGS INHTYHLDVVERSPHRPILQAGLPANASTVVGG DVEFVCKVYSDAQPHIQWIKHVEKNGSKYGPDGL PYLKVLKHSGINSSNAEVLALFNVTEADAGEYI CKVSNYIGQANQSAWLTVLPKQQAPGREKEITA SPDYLEIAIYCIGVFLIACMVVTVILCRMKNTTK KPDFSSQPAVHKLTKRIPLRRQVSAESSSSMNS NTPLVRITTRLSSTADTPMLAGVSEYELPEDPK WEFPRDKLTLGKPLGEGCFGQVVMAEAVGIDKDK PKEAVTVAVKMLKDDATEKDLSDLVSEMEMMKM IGKHKNIINLLGACTQDGPLYVIVEYASKGNLR EYLRARRPPGMEYSYDINRVPEEQMTFKDLVSCT YQLARGMEYLASQKCIHRDLAARNVLVTENNVM KIADFGLARDINNIDYYKKTTNGRLPVKWMAPE ALFDRVYTHQSDVWSFGVLMWEIFTLGGSPYPGI PVEELFKLLKEGHRMDKPANCTNELYMMMRDCW HAVPSQRPTFKQLVEDLDRILTLTTNEEYLDLS QPLEQYSPSYPDTRSSCSSGDDSVFSPDPMPYEP CLPQYPHINGSVKT*MTVSACPQTGQHWEPSYT EQGDHASQSLLSPLVYMDQRSK*LEK*SAYV*R FIQLKTCNLPQEEKKVSGAVDCHKPPCNPSHLPC VLAVDQ*DSRWTCVLPSLLIL**LEKIYVSTHL QSTNAVYRCWMYVNIFKLCINIYYIFTRSYFLY *F*MDVPMHLENWSLFF**LFAKCCSYT*FLNFH RAEVEKYFCFQGKWYNVNLLINW*YTKQLIIYS FFCNLSGISMQAAQQTS*SIAWT*LVIRSFEKR IFTIYD*FGENEVLIYLCLNAAVRRLFLDLLNAP Y*KNSFIGRCFILVCNPVITSTQRLTGLPKING TSVLLKDALIHSLRTDLS*NDSRMCFSLAAGLL LLSCTLIRLACILFSEF**WLPDSLALETPVRIF KSHHRKLKHRVVLLIVLGIRPSF*GIAFI*FWQ DLTKRSSLIPTSDKISPLFLLY*SIVFCFGNTH SLCNSRAR*MQITLILCVTKLEKVFNKTC*FLY* Q*KCFYRY*C*QDKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKMPSHPEMMRMTPMV RKILSVRTVTTREHHTGPTQKRWKSGSMLCLRP TLSSFAAQPGGTQCQPCGG*KTGRSLSRSIALE ATRYETSTGASLWKVWSHLTREIIPV*WRMNTGP SITRTTWMLWSDRLTGPSSKPDCRQMPPQWSEE T*SLSARFTVMPSPTSSGSSTWKRTAVNTGPTG CPTSRFSSTRG*IVPMQKCWLCSM*PRRMLGNIY VRSPII*GRPTSLPGSLSCQNSKRLEEKRRLQL PQTTWR*PFTA*GSS*SPVWW*QSSCAE*RTRP RSQTSAASRLCTS*PNVSPCGDRFRLSPAPP*TP TPRW*G*QHASLQRQTPPCWQGSPSMNFQRTQN GSFQEIS*HWASPWEKVALGKWSWRKQWELTKT SPRRRSPWP*RC*KMMPQRKTFLIWCQRWR**R* LGNTRIS*IFLEPAHRMGLSMS*LSMPLKATSE NTSEPGGHPGWSTPMTLTVFLRSR*PSRTWCHA PTSWPEAWSTWLPKNVFIEI*QPEMFW*QKTM** K*QTLDSPEISTI*TITKRPPMGGFQSSGWLQK PCLIEYTLIRVMSGPSGC*CGRSSL*GARPTQG FPWRNFLSC*RKDTEWISQPTAPTNCT***GTVG MQCPPRDQRSSSW*KTWIEFSLSQPMRNTWTSA NLSNSIHLVTLTQEVLVLQEMILFFLQTPCLTN HAFLSIHT*TAVLKHE*LCLPVPKQDSTGNLATL SRETMPPRACCLHLYIWIRGVNNWKSNQHMCKD LYS*KLVIFPRRRRRFLEQWTATSHHVTPLTCR AYWLWTSRTQGGRAFCLPC*FCNNWRRFMSAHTY RAQMQYIGAGCM*IYSNYV*IYIIYLQGVIFCI DFKWMSQCT*KIGLSFFNSYLLNAVLTHNFLIF TEQRWKNTFAFRENGITLIY**IGNIQNN*SFIV FFVI*VAFLCRQHSRLVNLLLGLN*LSDPLKRE YLQYMTNLGKMKF*FICV*MLLSDDCS*TS*MP HIKRTHS*EGVSFWCATLSLRQRNV*LDFPR*MV PASS*KMP*SIP*GQTLVEMIAECASLWQLAFC F*VAH*SD*PVFSSVNFDNGFQTLWRWRRLLGS SSPIIEN*NTELFC**FWGYVHLFKGLLSSNSGR TSPKDPASYLHQTKYRRCSFCTKVLCFALETPT HFAIAVQDECRLH*SYVLQNWRKYLIKPVNFYT DNKNVSTDINVNKTK*MSRNLFF*

Sequence FGFR2.gAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGT GCGGAAGATTTTGTCAGTGAGAACAGTAACAACAAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCA ACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGAACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGG CTACAAGGTACGAAACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCC ATCAATCACACGTACCACCTGGATGTTGTGGAGCGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAG ACGTAGAGTTTGTCTGCAAGGTTTACAGTGATGCCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCT GCCCTACCTCAAGGTTCTCAAGGCCGCCGGTGTTAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAACTTTTGAGGACGCTGGGGAA TATACGTGCTTGGCGGGTAATTCTATTGGGATATCCTTTCACTCTGCATGGTTGACAGTTCTGCCAGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCC CAGACTACCTGGAGATAGCCATTTACTGCATAGGGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAA GCCAGACTTCAGCAGCCAGCCGGCTGTGCACAAGCTGACCAAACGTATCCCCCTGCGGAGACAGGTAACAGTTTCGGCTGAGTCCAGCTCCTCCATGAAC TCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAA AATGGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGA CAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAG ATGATTGGGAAACACAAGAATATCATAAATCTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCC GAGAATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATG CACCTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTG ATGAAAATAGCAGACTTTGGACTCGCCAGAGATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAG AAGCCCTGTTTGATAGAGTATACACTCATCAGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGG GATTCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGT TGGCATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTCA GCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAGACCCCATGCCTTACGA ACCATGCCTTCCTCAGTATCCACACATAAACGGCAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCACTGGGAACCTAGCTAC ACTGAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAGTAATCAGCATATGTGTAAA GATTTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCATGTAACCCCTCTCACCTGCC GTGCGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAAGATTTATGTCAGCACACAC TTACAGAGCACAAATGCAGTATATAGGTGCTGGATGTATGTAAATATATTCAAATTATGTATAAATATATATTATATATTTACAAGGAGTTATTTTTTGT ATTGATTTTAAATGGATGTCCCAATGCACCTAGAAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTTACACATAATTTCTTAATTT TCACCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATACAAAACAATTAATCATTTAT AGTTTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTTATCAGATCCTTTGAAAAGA GAATATTTACAATATATGACTAATTTGGGGAAAATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCTTAGACCTCCTAAATGC CCCATATTAAAAGAACTCATTCATAGGAAGGTGTTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAACTGGACTTCCCAAGATAAAT GGTACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCTCTGGCAGCTGGCCTTC TGCTTCTGAGTTGCACATTAATCAGATTAGCCTGTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGGAGACGCCTGTTAGGAT CTTCAAGTCCCATCATAGAAAATTGAAACACAGAGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGATTGCTTTCATCTAATTCTGG CAGGACCTCACCAAAAGATCCAGCCTCATACCTACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTTTGCTTTGGAAACACCC ACTCACTTTGCAATAGCCGTGCAAGATGAATGCAGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATAAAACCTGTTAATTTTTATA CTGACAATAAAAATGTTTCTACAGATATTAATGTTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F1 (forward primer)
FGFR2.R1 (reverse primer)
Single exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRCHLIRR**G*HRW CGRFCQ*EQ*QQESTILDQHRKDGKAAPCCACGQ HCQVSLPSRGEPNANHAVAEKREGV*AGASHWR LQGTKPALEPHYGKCGPI*QGKLYLCSGE*IRV HQSHVPPGCCGAIASPAHPPSRTAGKCLHSGRRR RRVCLQGLQ*CPAPHPVDQARGKERQ*IRARRA ALPQGSQGRRC*HHGQRD*GSLYSECNF*GRWG IYVLGG*FYWDILSLCMVDSSASAWKRKGDYSFP RLPGDSHLLHRGLLNRLYGGNSHPVPNEEHDQE ARLQQPAGCAQADQTYPPAETGNSFG*VQLLHE LQHPAGEDNNTPLFNGRHPHAGRGLRV*TSRGPK MGVSKR*ADTGQAPGRRLLWASGHGGSSGN*QR QAQGGGHRGREDVER*CHRERPF*SGVRDGDDE DDWETQEYHKSSWSLHTGWASLCHS*VCL*RQPP RIPPSPEATRDGVLL*H*PCS*GADDLQGLGVM HLPAGQRHGVLGFPKMYSSRFSSQKCFGNRKQC DENSRLWTRQRYQQYRLLQKDHQWAASSQVDGSR SPV**SIHSSE*CLVLRGVNVGDLHFRGLALPR DSRGGTF*AAEGRTQNG*ASQLHQRTVHDDEGL LACSALPETNVQAVGRRLGSNSHSHNQ*GILGPQ PTSRTVFT*LP*HKKFLFFRR*FCFFSRPHALR TMPSSVSTHKRQC*NMNDCVCLSPNRTALGT*L H*AGRPCLPELVVSTCIYGSEE*IIGKVISICVK IYTVENL*SSPGGEEGFWSSGLPQATM*PLSPA VRTGCGPVGLKVDVRSAFLVNFVIIGEDLCQHT LTEHKCSI*VLDVCKYIQIMYKYILYIYKELFFV LILNGCPNAPRKLVSLFLIAIC*MLFLHIIS*F SPSRGGKILLLSGKMV*R*FINKLVIYKTINHL *FFL*FKWHFYAGSTAD*LIYCLDLTSYQIL*KE NIYNI*LIWGK*SFDLFVFKCCCQTIVLRPPKC PILKELIHRKVFHFGVQPCHYVNATSNWTSQDK WYQRPLKRCLNPFLEDRP*LK**QNVLLSGSWPS ASELHINQISLYSLQ*ILIMASRLFGVGDAC*D LQVPS*KIETQSCSADSFGDTSIFLRDCFHLIL AGPHQKIQPHTYIRQNIAVVPSVLKYCVLLWKHP LTLQ*PCKMNADYTDLMCYKIGESI**NLLIFI LTIKMFLQILMLTRQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEDAISSGDDEDDTDG AEDFVSENSNNKRAPYWTNTEKMEKRLHAVPAA NTVKFRCPAGGNPMPTMRWLKNGKEFKQEHRIGG YKVRNQHWSLIMESVVPSDKGNYTCVVENEYGS INHTYHLDVVERSPHRPILQAGLPANASTVVGG DVEFVCKVYSDAQPHIQWIKHVEKNGSKYGPDGL PYLKVLKAAGVNTTDKEIEVLYIRNVTFEDAGE YTCLAGNSIGISFHSAWLTVLPAPGREKEITAS PDYLEIAIYCIGVFLIACMVVTVILCRMKNTTKK PDFSSQPAVHKLTKRIPLRRQVTVSAESSSSMN SNTPLVRITTRLSSTADTPMLAGVSEYELPEDP KWEFPRDKLTLGKPLGEGCFGQVVMAEAVGIDKD KPKEAVTVAVKMLKDDATEKDLSDLVSEMEMMK MIGKHKNIINLLGACTQDGPLYVIVEYASKGNL REYLRARRPPGMEYSYDINRVPEEQMTFKDLVSC TYQLARGMEYLASQKCIHRDLAARNVLVTENNV MKIADFGLARDINNIDYYKKTTNGRLPVKWMAP EALFDRVYTHQSDVWSFGVLMWEIFTLGGSPYPG IPVEELFKLLKEGHRMDKPANCTNELYMMMRDC WHAVPSQRPTFKQLVEDLDRILTLTTNEEYLDL SQPLEQYSPSYPDTRSSCSSGDDSVFSPDPMPYE PCLPQYPHINGSVKT*MTVSACPQTGQHWEPSY TEQGDHASQSLLSPLVYMDQRSK*LEK*SAYV* RFIQLKTCNLPQEEKKVSGAVDCHKPPCNPSHLP CVLAVDQ*DSRWTCVLPSLLIL**LEKIYVSTH LQSTNAVYRCWMYVNIFKLCINIYYIFTRSYFL Y*F*MDVPMHLENWSLFF**LFAKCCSYT*FLNF HRAEVEKYFCFQGKWYNVNLLINW*YTKQLIIY SFFCNLSGISMQAAQQTS*SIAWT*LVIRSFEK RIFTIYD*FGENEVLIYLCLNAAVRRLFLDLLNA PY*KNSFIGRCFILVCNPVITSTQRLTGLPKIN GTSVLLKDALIHSLRTDLS*NDSRMCFSLAAGL LLLSCTLIRLACILFSEF**WLPDSLALETPVRI FKSHHRKLKHRVVLLIVLGIRPSF*GIAFI*FW QDLTKRSSLIPTSDKISPLFLLY*SIVFCFGNT HSLCNSRAR*MQITLILCVTKLEKVFNKTC*FLY *Q*KCFYRY*C*QDKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKMPSHPEMMRMTPMV RKILSVRTVTTREHHTGPTQKRWKSGSMLCLRP TLSSFAAQPGGTQCQPCGG*KTGRSLSRSIALE ATRYETSTGASLWKVWSHLTREIIPV*WRMNTGP SITRTTWMLWSDRLTGPSSKPDCRQMPPQWSEE T*SLSARFTVMPSPTSSGSSTWKRTAVNTGPTG CPTSRFSRPPVLTPRTKRLRFSIFGM*LLRTLGN IRAWRVILLGYPFTLHG*QFCQRLEEKRRLQLP QTTWR*PFTA*GSS*SPVWW*QSSCAE*RTRPR SQTSAASRLCTS*PNVSPCGDR*QFRLSPAPP*T PTPRW*G*QHASLQRQTPPCWQGSPSMNFQRTQ NGSFQEIS*HWASPWEKVALGKWSWRKQWELTK TSPRRRSPWP*RC*KMMPQRKTFLIWCQRWR**R *LGNTRIS*IFLEPAHRMGLSMS*LSMPLKATS ENTSEPGGHPGWSTPMTLTVFLRSR*PSRTWCH APTSWPEAWSTWLPKNVFIEI*QPEMFW*QKTM* *K*QTLDSPEISTI*TITKRPPMGGFQSSGWLQ KPCLIEYTLIRVMSGPSGC*CGRSSL*GARPTQ GFPWRNFLSC*RKDTEWISQPTAPTNCT***GTV GMQCPPRDQRSSSW*KTWIEFSLSQPMRNTWTS ANLSNSIHLVTLTQEVLVLQEMILFFLQTPCLT NHAFLSIHT*TAVLKHE*LCLPVPKQDSTGNLAT LSRETMPPRACCLHLYIWIRGVNNWKSNQHMCK DLYS*KLVIFPRRRRRFLEQWTATSHHVTPLTC RAYWLWTSRTQGGRAFCLPC*FCNNWRRFMSAHT YRAQMQYIGAGCM*IYSNYV*IYIIYLQGVIFC IDFKWMSQCT*KIGLSFFNSYLLNAVLTHNFLI FTEQRWKNTFAFRENGITLIY**IGNIQNN*SFI VFFVI*VAFLCRQHSRLVNLLLGLN*LSDPLKR EYLQYMTNLGKMKF*FICV*MLLSDDCS*TS*M PHIKRTHS*EGVSFWCATLSLRQRNV*LDFPR*M VPASS*KMP*SIP*GQTLVEMIAECASLWQLAF CF*VAH*SD*PVFSSVNFDNGFQTLWRWRRLLG SSSPIIEN*NTELFC**FWGYVHLFKGLLSSNSG RTSPKDPASYLHQTKYRRCSFCTKVLCFALETP THFAIAVQDECRLH*SYVLQNWRKYLIKPVNFY TDNKNVSTDINVNKTK*MSRNLFF*

Sequence FGFR2.rAug05

FGFR2.F1 (forward primer)
FGFR2.R1 (reverse primer)
Single exon skipping

Frame 0

Frame 1

Frame 2

Sequence FGFR2.qAug05

FGFR2.F1 (forward primer)
FGFR2.R1 (reverse primer)
Single exon skipping

Frame 0

Frame 1

Frame 2

Sequence FGFR2.nAug05

AGGAAGTGTGCAGATGGGATTAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTG GTCGTGGTCACCATGGCAACCTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGAGCCACCAACCAAATACCAAA TCTCTCAACCAGAAGTGTACGTGGCTGCGCCAGGGGAGTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGGATGGGGT GCACTTGGGGCCCAACAATAGGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGGCCTCTATGCTTGTACTGCCAGT AGGACTGTAGACAGTGAAACTTGGTACTTCATGGTGAATGTCACAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGTGCGGAAGATTTTG TCAGTGAGAACAGTAACAACAAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTT TCGCTGCCCAGCCGGGGGGAACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTACAAGGTACGA AACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCCATCAATCACACGT ACCACCTGGATGTTGTGGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAGGGGTCTTCTTAATCGC CTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAAGCTGACCAAACGTATC CCCCTGCGGAGACAGGTAACAGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCTTCAACGGCAG ACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGCCCCTGGGAGA AGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTTGAAAGATGAT GCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTTCTTGGAGCCTGCACAC AGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGTACTCCTATGA CATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTCCCAAAAATGT ATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGATATCAACAATATAGACT ATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGAGTGATGTCTGGTCCTT CGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGGACACAGAATG GATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAGTTGGTAGAAG ACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGTTC TTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAACGGCAGTGTTAAAACATGA ATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCTCCACTTGTAT ATATGGATCAGAGGAGTAAATAATTGGAAAAGTAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGGAGAAGAAGGT TTCTGGAGCAGTGGACTGCCACAAGCCACCATGTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGACGTGCGTTCTG CCTTCCTTGTTAATTTTGTAATAATTGGAGAAGATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGCTGGATGTATGTAAATATAT TCAAATTATGTATAAATATATATTATATATTTACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACCTAGAAAATTGGTCTCTCTT TTTTTAATAGCTATTTGCTAAATGCTGTTCTTACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGGAAAATGGTAT AACGTTAATTTATTAATAAATTGGTAATATACAAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCAGCACAGCAGA CTAGTTAATCTATTGCTTGGACTTAACTAGTTATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGGAAAATGAAGTTTTGATTTA TTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAGGTGTTTCATTTTGGTGTGC AACCCTGTCATTACGTCAACGCAACGTCTAACTGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCCTTGAGGACAG ACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAGCCTGTATTCTCTTCAGTGA ATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACACAGAGTTGTTCTGCTGATA GTTTTGGGGATACGTCCATCTTTTTAAGGGATTGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATACCTACATCAGACAAAATAT CGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAATGCAGATTACACTGATCTT ATGTGTTACAAAATTGGAGAAAGTATTTAATAAAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTAATGTTAACAAGACAAAATA AATGTCACGCAACTTATTTTTTTAA

FGFR2.F1 (forward primer)
FGFR2.R1 (reverse primer)
Single exon skipping

Frame 0

RKCADGINVHMEIWKRTGDWYRNHGQLGSFHLP GRGHHGNLVPGPALLQFS*GYHIRARRATNQIPN LSTRSVRGCARGVARGALPVERCRRDQLD*GWG ALGAQQ*DSAYWGVLADKGRHA*RLRPLCLYCQ *DCRQ*NLVLHGECHRCHLIRR**G*HRWCGRFC Q*EQ*QQESTILDQHRKDGKAAPCCACGQHCQV SLPSRGEPNANHAVAEKREGV*AGASHWRLQGT KPALEPHYGKCGPI*QGKLYLCSGE*IRVHQSHV PPGCCGAWKRKGDYSFPRLPGDSHLLHRGLLNR LYGGNSHPVPNEEHDQEARLQQPAGCAQADQTY PPAETGNSFG*VQLLHELQHPAGEDNNTPLFNGR HPHAGRGLRV*TSRGPKMGVSKR*ADTGQAPGR RLLWASGHGGSSGN*QRQAQGGGHRGREDVER* CHRERPF*SGVRDGDDEDDWETQEYHKSSWSLHT GWASLCHS*VCL*RQPPRIPPSPEATRDGVLL* H*PCS*GADDLQGLGVMHLPAGQRHGVLGFPKM YSSRFSSQKCFGNRKQCDENSRLWTRQRYQQYRL LQKDHQWAASSQVDGSRSPV**SIHSSE*CLVL RGVNVGDLHFRGLALPRDSRGGTF*AAEGRTQN G*ASQLHQRTVHDDEGLLACSALPETNVQAVGRR LGSNSHSHNQ*GILGPQPTSRTVFT*LP*HKKF LFFRR*FCFFSRPHALRTMPSSVSTHKRQC*NM NDCVCLSPNRTALGT*LH*AGRPCLPELVVSTCI YGSEE*IIGKVISICVKIYTVENL*SSPGGEEG FWSSGLPQATM*PLSPAVRTGCGPVGLKVDVRS AFLVNFVIIGEDLCQHTLTEHKCSI*VLDVCKYI QIMYKYILYIYKELFFVLILNGCPNAPRKLVSL FLIAIC*MLFLHIIS*FSPSRGGKILLLSGKMV *R*FINKLVIYKTINHL*FFL*FKWHFYAGSTAD *LIYCLDLTSYQIL*KENIYNI*LIWGK*SFDL FVFKCCCQTIVLRPPKCPILKELIHRKVFHFGV QPCHYVNATSNWTSQDKWYQRPLKRCLNPFLEDR P*LK**QNVLLSGSWPSASELHINQISLYSLQ* ILIMASRLFGVGDAC*DLQVPS*KIETQSCSAD SFGDTSIFLRDCFHLILAGPHQKIQPHTYIRQNI AVVPSVLKYCVLLWKHPLTLQ*PCKMNADYTDL MCYKIGESI**NLLIFILTIKMFLQILMLTRQN KCHATYFF

Frame 1

GSVQMGLTSTWRYGRGPGIGTVTMVSWGRFICL VVVTMATLSLARPSFSLVEDTTLEPEEPPTKYQ ISQPEVYVAAPGESLEVRCLLKDAAVISWTKDGV HLGPNNRTVLIGEYLQIKGATPRDSGLYACTAS RTVDSETWYFMVNVTDAISSGDDEDDTDGAEDF VSENSNNKRAPYWTNTEKMEKRLHAVPAANTVKF RCPAGGNPMPTMRWLKNGKEFKQEHRIGGYKVR NQHWSLIMESVVPSDKGNYTCVVENEYGSINHT YHLDVVAPGREKEITASPDYLEIAIYCIGVFLIA CMVVTVILCRMKNTTKKPDFSSQPAVHKLTKRI PLRRQVTVSAESSSSMNSNTPLVRITTRLSSTA DTPMLAGVSEYELPEDPKWEFPRDKLTLGKPLGE GCFGQVVMAEAVGIDKDKPKEAVTVAVKMLKDD ATEKDLSDLVSEMEMMKMIGKHKNIINLLGACT QDGPLYVIVEYASKGNLREYLRARRPPGMEYSYD INRVPEEQMTFKDLVSCTYQLARGMEYLASQKC IHRDLAARNVLVTENNVMKIADFGLARDINNID YYKKTTNGRLPVKWMAPEALFDRVYTHQSDVWSF GVLMWEIFTLGGSPYPGIPVEELFKLLKEGHRM DKPANCTNELYMMMRDCWHAVPSQRPTFKQLVE DLDRILTLTTNEEYLDLSQPLEQYSPSYPDTRSS CSSGDDSVFSPDPMPYEPCLPQYPHINGSVKT* MTVSACPQTGQHWEPSYTEQGDHASQSLLSPLV YMDQRSK*LEK*SAYV*RFIQLKTCNLPQEEKKV SGAVDCHKPPCNPSHLPCVLAVDQ*DSRWTCVL PSLLIL**LEKIYVSTHLQSTNAVYRCWMYVNI FKLCINIYYIFTRSYFLY*F*MDVPMHLENWSLF F**LFAKCCSYT*FLNFHRAEVEKYFCFQGKWY NVNLLINW*YTKQLIIYSFFCNLSGISMQAAQQ TS*SIAWT*LVIRSFEKRIFTIYD*FGENEVLIY LCLNAAVRRLFLDLLNAPY*KNSFIGRCFILVC NPVITSTQRLTGLPKINGTSVLLKDALIHSLRT DLS*NDSRMCFSLAAGLLLLSCTLIRLACILFSE F**WLPDSLALETPVRIFKSHHRKLKHRVVLLI VLGIRPSF*GIAFI*FWQDLTKRSSLIPTSDKI SPLFLLY*SIVFCFGNTHSLCNSRAR*MQITLIL CVTKLEKVFNKTC*FLY*Q*KCFYRY*C*QDKI NVTQLIFL

Frame 2

EVCRWD*RPHGDMEEDRGLVP*PWSAGVVSSAW SWSPWQPCPWPGPPSV*LRIPH*SQKSHQPNTK SLNQKCTWLRQGSR*RCAAC*KMPP*SVGLRMG CTWGPTIGQCLLGSTCR*RAPRLETPASMLVLPV GL*TVKLGTSW*MSQMPSHPEMMRMTPMVRKIL SVRTVTTREHHTGPTQKRWKSGSMLCLRPTLSS FAAQPGGTQCQPCGG*KTGRSLSRSIALEATRYE TSTGASLWKVWSHLTREIIPV*WRMNTGPSITR TTWMLWRLEEKRRLQLPQTTWR*PFTA*GSS*S PVWW*QSSCAE*RTRPRSQTSAASRLCTS*PNVS PCGDR*QFRLSPAPP*TPTPRW*G*QHASLQRQ TPPCWQGSPSMNFQRTQNGSFQEIS*HWASPWE KVALGKWSWRKQWELTKTSPRRRSPWP*RC*KMM PQRKTFLIWCQRWR**R*LGNTRIS*IFLEPAH RMGLSMS*LSMPLKATSENTSEPGGHPGWSTPM TLTVFLRSR*PSRTWCHAPTSWPEAWSTWLPKNV FIEI*QPEMFW*QKTM**K*QTLDSPEISTI*T ITKRPPMGGFQSSGWLQKPCLIEYTLIRVMSGP SGC*CGRSSL*GARPTQGFPWRNFLSC*RKDTEW ISQPTAPTNCT***GTVGMQCPPRDQRSSSW*K TWIEFSLSQPMRNTWTSANLSNSIHLVTLTQEV LVLQEMILFFLQTPCLTNHAFLSIHT*TAVLKHE *LCLPVPKQDSTGNLATLSRETMPPRACCLHLY IWIRGVNNWKSNQHMCKDLYS*KLVIFPRRRRR FLEQWTATSHHVTPLTCRAYWLWTSRTQGGRAFC LPC*FCNNWRRFMSAHTYRAQMQYIGAGCM*IY SNYV*IYIIYLQGVIFCIDFKWMSQCT*KIGLS FFNSYLLNAVLTHNFLIFTEQRWKNTFAFRENGI TLIY**IGNIQNN*SFIVFFVI*VAFLCRQHSR LVNLLLGLN*LSDPLKREYLQYMTNLGKMKF*F ICV*MLLSDDCS*TS*MPHIKRTHS*EGVSFWCA TLSLRQRNV*LDFPR*MVPASS*KMP*SIP*GQ TLVEMIAECASLWQLAFCF*VAH*SD*PVFSSV NFDNGFQTLWRWRRLLGSSSPIIEN*NTELFC** FWGYVHLFKGLLSSNSGRTSPKDPASYLHQTKY RRCSFCTKVLCFALETPTHFAIAVQDECRLH*S YVLQNWRKYLIKPVNFYTDNKNVSTDINVNKTK* MSRNLFF*

Sequence FGFR2.jAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGAGCCACCAACCAAATACCAAATCTCTCAACCAGAAGTGTAC GTGGCTGCGCCAGGGGAGTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGGATGGGGTGCACTTGGGGCCCAACAATA GGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGGCCTCTATGCTTGTACTGCCAGTAGGACTGTAGACAGTGAAAC TTGGTACTTCATGGTGAATGTCACAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGTGCGGAAGATTTTGTCAGTGAGAACAGTAACAAC AAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGA ACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCAT TATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAG CGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATG CCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGGTTTCGGCTGA GTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTAT GAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAG CAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTC AGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTAT GCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCT TCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTT GGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCA GTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAG GGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTA CATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAAT GAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTC CAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAACGGCAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACA GCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAG TAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCAT GTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAA GATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGCTGGATGTATGTAAATATATTCAAATTATGTATAAATATATATTATATATTT ACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACCTAGAAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTT ACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATAC AAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTT ATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGGAAAATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGT TCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAGGTGTTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAAC TGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTC TCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAGCCTGTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGT TGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACACAGAGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGAT TGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATACCTACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTG TTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAATGCAGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATA AAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTAATGTTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F1 (forward primer)
FGFR2.R1 (reverse primer)
Single exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRATNQIPNLSTRSV RGCARGVARGALPVERCRRDQLD*GWGALGAQQ* DSAYWGVLADKGRHA*RLRPLCLYCQ*DCRQ*N LVLHGECHRCHLIRR**G*HRWCGRFCQ*EQ*Q QESTILDQHRKDGKAAPCCACGQHCQVSLPSRGE PNANHAVAEKREGV*AGASHWRLQGTKPALEPH YGKCGPI*QGKLYLCSGE*IRVHQSHVPPGCCG AIASPAHPPSRTAGKCLHSGRRRRRVCLQGLQ*C PAPHPVDQARGKERQ*IRARRAALPQGSQGFG* VQLLHELQHPAGEDNNTPLFNGRHPHAGRGLRV *TSRGPKMGVSKR*ADTGQAPGRRLLWASGHGGS SGN*QRQAQGGGHRGREDVER*CHRERPF*SGV RDGDDEDDWETQEYHKSSWSLHTGWASLCHS*V CL*RQPPRIPPSPEATRDGVLL*H*PCS*GADDL QGLGVMHLPAGQRHGVLGFPKMYSSRFSSQKCF GNRKQCDENSRLWTRQRYQQYRLLQKDHQWAAS SQVDGSRSPV**SIHSSE*CLVLRGVNVGDLHFR GLALPRDSRGGTF*AAEGRTQNG*ASQLHQRTV HDDEGLLACSALPETNVQAVGRRLGSNSHSHNQ *GILGPQPTSRTVFT*LP*HKKFLFFRR*FCFFS RPHALRTMPSSVSTHKRQC*NMNDCVCLSPNRT ALGT*LH*AGRPCLPELVVSTCIYGSEE*IIGK VISICVKIYTVENL*SSPGGEEGFWSSGLPQATM *PLSPAVRTGCGPVGLKVDVRSAFLVNFVIIGE DLCQHTLTEHKCSI*VLDVCKYIQIMYKYILYI YKELFFVLILNGCPNAPRKLVSLFLIAIC*MLFL HIIS*FSPSRGGKILLLSGKMV*R*FINKLVIY KTINHL*FFL*FKWHFYAGSTAD*LIYCLDLTS YQIL*KENIYNI*LIWGK*SFDLFVFKCCCQTIV LRPPKCPILKELIHRKVFHFGVQPCHYVNATSN WTSQDKWYQRPLKRCLNPFLEDRP*LK**QNVL LSGSWPSASELHINQISLYSLQ*ILIMASRLFGV GDAC*DLQVPS*KIETQSCSADSFGDTSIFLRD CFHLILAGPHQKIQPHTYIRQNIAVVPSVLKYC VLLWKHPLTLQ*PCKMNADYTDLMCYKIGESI** NLLIFILTIKMFLQILMLTRQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEEPPTKYQISQPEVY VAAPGESLEVRCLLKDAAVISWTKDGVHLGPNN RTVLIGEYLQIKGATPRDSGLYACTASRTVDSET WYFMVNVTDAISSGDDEDDTDGAEDFVSENSNN KRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGG NPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLI MESVVPSDKGNYTCVVENEYGSINHTYHLDVVE RSPHRPILQAGLPANASTVVGGDVEFVCKVYSD AQPHIQWIKHVEKNGSKYGPDGLPYLKVLKVSAE SSSSMNSNTPLVRITTRLSSTADTPMLAGVSEY ELPEDPKWEFPRDKLTLGKPLGEGCFGQVVMAE AVGIDKDKPKEAVTVAVKMLKDDATEKDLSDLVS EMEMMKMIGKHKNIINLLGACTQDGPLYVIVEY ASKGNLREYLRARRPPGMEYSYDINRVPEEQMT FKDLVSCTYQLARGMEYLASQKCIHRDLAARNVL VTENNVMKIADFGLARDINNIDYYKKTTNGRLP VKWMAPEALFDRVYTHQSDVWSFGVLMWEIFTL GGSPYPGIPVEELFKLLKEGHRMDKPANCTNELY MMMRDCWHAVPSQRPTFKQLVEDLDRILTLTTN EEYLDLSQPLEQYSPSYPDTRSSCSSGDDSVFS PDPMPYEPCLPQYPHINGSVKT*MTVSACPQTGQ HWEPSYTEQGDHASQSLLSPLVYMDQRSK*LEK *SAYV*RFIQLKTCNLPQEEKKVSGAVDCHKPP CNPSHLPCVLAVDQ*DSRWTCVLPSLLIL**LEK IYVSTHLQSTNAVYRCWMYVNIFKLCINIYYIF TRSYFLY*F*MDVPMHLENWSLFF**LFAKCCS YT*FLNFHRAEVEKYFCFQGKWYNVNLLINW*YT KQLIIYSFFCNLSGISMQAAQQTS*SIAWT*LV IRSFEKRIFTIYD*FGENEVLIYLCLNAAVRRL FLDLLNAPY*KNSFIGRCFILVCNPVITSTQRLT GLPKINGTSVLLKDALIHSLRTDLS*NDSRMCF SLAAGLLLLSCTLIRLACILFSEF**WLPDSLA LETPVRIFKSHHRKLKHRVVLLIVLGIRPSF*GI AFI*FWQDLTKRSSLIPTSDKISPLFLLY*SIV FCFGNTHSLCNSRAR*MQITLILCVTKLEKVFN KTC*FLY*Q*KCFYRY*C*QDKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKSHQPNTKSLNQKCT WLRQGSR*RCAAC*KMPP*SVGLRMGCTWGPTI GQCLLGSTCR*RAPRLETPASMLVLPVGL*TVK LGTSW*MSQMPSHPEMMRMTPMVRKILSVRTVTT REHHTGPTQKRWKSGSMLCLRPTLSSFAAQPGG TQCQPCGG*KTGRSLSRSIALEATRYETSTGAS LWKVWSHLTREIIPV*WRMNTGPSITRTTWMLWS DRLTGPSSKPDCRQMPPQWSEET*SLSARFTVM PSPTSSGSSTWKRTAVNTGPTGCPTSRFSRFRL SPAPP*TPTPRW*G*QHASLQRQTPPCWQGSPSM NFQRTQNGSFQEIS*HWASPWEKVALGKWSWRK QWELTKTSPRRRSPWP*RC*KMMPQRKTFLIWC QRWR**R*LGNTRIS*IFLEPAHRMGLSMS*LSM PLKATSENTSEPGGHPGWSTPMTLTVFLRSR*P SRTWCHAPTSWPEAWSTWLPKNVFIEI*QPEMF W*QKTM**K*QTLDSPEISTI*TITKRPPMGGFQ SSGWLQKPCLIEYTLIRVMSGPSGC*CGRSSL* GARPTQGFPWRNFLSC*RKDTEWISQPTAPTNC T***GTVGMQCPPRDQRSSSW*KTWIEFSLSQPM RNTWTSANLSNSIHLVTLTQEVLVLQEMILFFL QTPCLTNHAFLSIHT*TAVLKHE*LCLPVPKQD STGNLATLSRETMPPRACCLHLYIWIRGVNNWKS NQHMCKDLYS*KLVIFPRRRRRFLEQWTATSHH VTPLTCRAYWLWTSRTQGGRAFCLPC*FCNNWR RFMSAHTYRAQMQYIGAGCM*IYSNYV*IYIIYL QGVIFCIDFKWMSQCT*KIGLSFFNSYLLNAVL THNFLIFTEQRWKNTFAFRENGITLIY**IGNI QNN*SFIVFFVI*VAFLCRQHSRLVNLLLGLN*L SDPLKREYLQYMTNLGKMKF*FICV*MLLSDDC S*TS*MPHIKRTHS*EGVSFWCATLSLRQRNV* LDFPR*MVPASS*KMP*SIP*GQTLVEMIAECAS LWQLAFCF*VAH*SD*PVFSSVNFDNGFQTLWR WRRLLGSSSPIIEN*NTELFC**FWGYVHLFKG LLSSNSGRTSPKDPASYLHQTKYRRCSFCTKVLC FALETPTHFAIAVQDECRLH*SYVLQNWRKYLI KPVNFYTDNKNVSTDINVNKTK*MSRNLFF*

Sequence FGFR2.fAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGAGCCACCAACCAAATACCAAATCTCTCAACCAGAAGTGTAC GTGGCTGCGCCAGGGGAGTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGGATGGGGTGCACTTGGGGCCCAACAATA GGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGGCCTCTATGCTTGTACTGCCAGTAGGACTGTAGACAGTGAAAC TTGGTACTTCATGGTGAATGTCACAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGTGCGGAAGATTTTGTCAGTGAGAACAGTAACAAC AAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGA ACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCAT TATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAG CGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATG CCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGGCCGCCGGTGT TAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAACTTTTGAGGACGCTGGGGAATATACGTGCTTGGCGGGTAATTCTATTGGGATA TCCTTTCACTCTGCATGGTTGACAGTTCTGCCAGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAG GGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAA GCTGACCAAACGTATCCCCCTGCGGAGACAGGTAACAGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGC CTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGG GCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAA GATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTT CTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGA TGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTT GGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGAT ATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGA GTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAA GGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTC AAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGATCTGAAAGTTTATGGCTTCATTGAGAAACTGGGAAAAGTTGGTCAGGC GCAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGCGGATCATGAGGTCAGGAGTTCCAGACCAGCCTGGCCAACATGGTGAAACCC TGTCTCTACTAAAGATACAAAAAATTAGCCGGGCGTGTTGGTGTGCACCTGTAATCCCAGCTACTCCGGGAGGCTGAGGCAGGAGAGTCACTTGAACCGG GGAGGCGGAGGTTGCAGTGAGCCGAGATCATGCCATTGCATTCCAGCCTTGGCGACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAAT

FGFR2.F1 (forward primer)
FGFR2.R1 (reverse primer)
Single exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRATNQIPNLSTRSV RGCARGVARGALPVERCRRDQLD*GWGALGAQQ* DSAYWGVLADKGRHA*RLRPLCLYCQ*DCRQ*N LVLHGECHRCHLIRR**G*HRWCGRFCQ*EQ*Q QESTILDQHRKDGKAAPCCACGQHCQVSLPSRGE PNANHAVAEKREGV*AGASHWRLQGTKPALEPH YGKCGPI*QGKLYLCSGE*IRVHQSHVPPGCCG AIASPAHPPSRTAGKCLHSGRRRRRVCLQGLQ*C PAPHPVDQARGKERQ*IRARRAALPQGSQGRRC *HHGQRD*GSLYSECNF*GRWGIYVLGG*FYWD ILSLCMVDSSASAWKRKGDYSFPRLPGDSHLLHR GLLNRLYGGNSHPVPNEEHDQEARLQQPAGCAQ ADQTYPPAETGNSFG*VQLLHELQHPAGEDNNT PLFNGRHPHAGRGLRV*TSRGPKMGVSKR*ADTG QAPGRRLLWASGHGGSSGN*QRQAQGGGHRGRE DVER*CHRERPF*SGVRDGDDEDDWETQEYHKS SWSLHTGWASLCHS*VCL*RQPPRIPPSPEATRD GVLL*H*PCS*GADDLQGLGVMHLPAGQRHGVL GFPKMYSSRFSSQKCFGNRKQCDENSRLWTRQR YQQYRLLQKDHQWAASSQVDGSRSPV**SIHSSE *CLVLRGVNVGDLHFRGLALPRDSRGGTF*AAE GRTQNG*ASQLHQRTVHDDEGLLACSALPETNV QAVGRRLGSNSHSHNQ*DLKVYGFIEKLGKVGQA QWLMPVIPALWEAEAGGS*GQEFQTSLANMVKP CLY*RYKKLAGRVGVHL*SQLLREAEAGESLEP GRRRLQ*AEIMPLHSSLGDRARLRLKKKKN

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEEPPTKYQISQPEVY VAAPGESLEVRCLLKDAAVISWTKDGVHLGPNN RTVLIGEYLQIKGATPRDSGLYACTASRTVDSET WYFMVNVTDAISSGDDEDDTDGAEDFVSENSNN KRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGG NPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLI MESVVPSDKGNYTCVVENEYGSINHTYHLDVVE RSPHRPILQAGLPANASTVVGGDVEFVCKVYSD AQPHIQWIKHVEKNGSKYGPDGLPYLKVLKAAGV NTTDKEIEVLYIRNVTFEDAGEYTCLAGNSIGI SFHSAWLTVLPAPGREKEITASPDYLEIAIYCI GVFLIACMVVTVILCRMKNTTKKPDFSSQPAVHK LTKRIPLRRQVTVSAESSSSMNSNTPLVRITTR LSSTADTPMLAGVSEYELPEDPKWEFPRDKLTL GKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVK MLKDDATEKDLSDLVSEMEMMKMIGKHKNIINL LGACTQDGPLYVIVEYASKGNLREYLRARRPPG MEYSYDINRVPEEQMTFKDLVSCTYQLARGMEYL ASQKCIHRDLAARNVLVTENNVMKIADFGLARD INNIDYYKKTTNGRLPVKWMAPEALFDRVYTHQ SDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLLK EGHRMDKPANCTNELYMMMRDCWHAVPSQRPTF KQLVEDLDRILTLTTNEI*KFMASLRNWEKLVR RSGSCL*SQHFGRPRQADHEVRSSRPAWPTW*NP VSTKDTKN*PGVLVCTCNPSYSGRLRQESHLNR GGGGCSEPRSCHCIPALATERDSVSKKKK

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKSHQPNTKSLNQKCT WLRQGSR*RCAAC*KMPP*SVGLRMGCTWGPTI GQCLLGSTCR*RAPRLETPASMLVLPVGL*TVK LGTSW*MSQMPSHPEMMRMTPMVRKILSVRTVTT REHHTGPTQKRWKSGSMLCLRPTLSSFAAQPGG TQCQPCGG*KTGRSLSRSIALEATRYETSTGAS LWKVWSHLTREIIPV*WRMNTGPSITRTTWMLWS DRLTGPSSKPDCRQMPPQWSEET*SLSARFTVM PSPTSSGSSTWKRTAVNTGPTGCPTSRFSRPPV LTPRTKRLRFSIFGM*LLRTLGNIRAWRVILLGY PFTLHG*QFCQRLEEKRRLQLPQTTWR*PFTA* GSS*SPVWW*QSSCAE*RTRPRSQTSAASRLCT S*PNVSPCGDR*QFRLSPAPP*TPTPRW*G*QHA SLQRQTPPCWQGSPSMNFQRTQNGSFQEIS*HW ASPWEKVALGKWSWRKQWELTKTSPRRRSPWP* RC*KMMPQRKTFLIWCQRWR**R*LGNTRIS*IF LEPAHRMGLSMS*LSMPLKATSENTSEPGGHPG WSTPMTLTVFLRSR*PSRTWCHAPTSWPEAWST WLPKNVFIEI*QPEMFW*QKTM**K*QTLDSPEI STI*TITKRPPMGGFQSSGWLQKPCLIEYTLIR VMSGPSGC*CGRSSL*GARPTQGFPWRNFLSC* RKDTEWISQPTAPTNCT***GTVGMQCPPRDQRS SSW*KTWIEFSLSQPMRSESLWLH*ETGKSWSG AVAHACNPSTLGGRGRRIMRSGVPDQPGQHGET LSLLKIQKISRACWCAPVIPATPGG*GRRVT*TG EAEVAVSRDHAIAFQPWRQSETPSQKKKK

Sequence FGFR2.eAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGAGCCACCAACCAAATACCAAATCTCTCAACCAGAAGTGTAC GTGGCTGCGCCAGGGGAGTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGGATGGGGTGCACTTGGGGCCCAACAATA GGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGGCCTCTATGCTTGTACTGCCAGTAGGACTGTAGACAGTGAAAC TTGGTACTTCATGGTGAATGTCACAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGTGCGGAAGATTTTGTCAGTGAGAACAGTAACAAC AAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGA ACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCAT TATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAG CGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATG CCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGCACTCGGGGAT AAATAGTTCCAATGCAGAAGTGCTGGCTCTGTTCAATGTGACCGAGGCGGATGCTGGGGAATATATATGTAAGGTCTCCAATTATATAGGGCAGGCCAAC CAGTCTGCCTGGCTCACTGTCCTGCCAAAACAGCAAGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCA TAGGGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCA CAAGCTGACCAAACGTATCCCCCTGCGGAGACAGGTAACAGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACA CGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACAC TGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGT GAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAAT CTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCG GGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTA CTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGA GATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATC AGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCT GAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACG TTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGATCTGAAAGTTTATGGCTTCATTGAGAAACTGGGAAAAGTTGGTCA GGCGCAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGCGGATCATGAGGTCAGGAGTTCCAGACCAGCCTGGCCAACATGGTGAAA CCCTGTCTCTACTAAAGATACAAAAAATTAGCCGGGCGTGTTGGTGTGCACCTGTAATCCCAGCTACTCCGGGAGGCTGAGGCAGGAGAGTCACTTGAAC CGGGGAGGCGGAGGTTGCAGTGAGCCGAGATCATGCCATTGCATTCCAGCCTTGGCGACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAAT

FGFR2.F1 (forward primer)
FGFR2.R1 (reverse primer)
Single exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRATNQIPNLSTRSV RGCARGVARGALPVERCRRDQLD*GWGALGAQQ* DSAYWGVLADKGRHA*RLRPLCLYCQ*DCRQ*N LVLHGECHRCHLIRR**G*HRWCGRFCQ*EQ*Q QESTILDQHRKDGKAAPCCACGQHCQVSLPSRGE PNANHAVAEKREGV*AGASHWRLQGTKPALEPH YGKCGPI*QGKLYLCSGE*IRVHQSHVPPGCCG AIASPAHPPSRTAGKCLHSGRRRRRVCLQGLQ*C PAPHPVDQARGKERQ*IRARRAALPQGSQALGD K*FQCRSAGSVQCDRGGCWGIYM*GLQLYRAGQ PVCLAHCPAKTASAWKRKGDYSFPRLPGDSHLLH RGLLNRLYGGNSHPVPNEEHDQEARLQQPAGCA QADQTYPPAETGNSFG*VQLLHELQHPAGEDNN TPLFNGRHPHAGRGLRV*TSRGPKMGVSKR*ADT GQAPGRRLLWASGHGGSSGN*QRQAQGGGHRGR EDVER*CHRERPF*SGVRDGDDEDDWETQEYHK SSWSLHTGWASLCHS*VCL*RQPPRIPPSPEATR DGVLL*H*PCS*GADDLQGLGVMHLPAGQRHGV LGFPKMYSSRFSSQKCFGNRKQCDENSRLWTRQ RYQQYRLLQKDHQWAASSQVDGSRSPV**SIHSS E*CLVLRGVNVGDLHFRGLALPRDSRGGTF*AA EGRTQNG*ASQLHQRTVHDDEGLLACSALPETN VQAVGRRLGSNSHSHNQ*DLKVYGFIEKLGKVGQ AQWLMPVIPALWEAEAGGS*GQEFQTSLANMVK PCLY*RYKKLAGRVGVHL*SQLLREAEAGESLE PGRRRLQ*AEIMPLHSSLGDRARLRLKKKKN

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEEPPTKYQISQPEVY VAAPGESLEVRCLLKDAAVISWTKDGVHLGPNN RTVLIGEYLQIKGATPRDSGLYACTASRTVDSET WYFMVNVTDAISSGDDEDDTDGAEDFVSENSNN KRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGG NPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLI MESVVPSDKGNYTCVVENEYGSINHTYHLDVVE RSPHRPILQAGLPANASTVVGGDVEFVCKVYSD AQPHIQWIKHVEKNGSKYGPDGLPYLKVLKHSGI NSSNAEVLALFNVTEADAGEYICKVSNYIGQAN QSAWLTVLPKQQAPGREKEITASPDYLEIAIYC IGVFLIACMVVTVILCRMKNTTKKPDFSSQPAVH KLTKRIPLRRQVTVSAESSSSMNSNTPLVRITT RLSSTADTPMLAGVSEYELPEDPKWEFPRDKLT LGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAV KMLKDDATEKDLSDLVSEMEMMKMIGKHKNIIN LLGACTQDGPLYVIVEYASKGNLREYLRARRPP GMEYSYDINRVPEEQMTFKDLVSCTYQLARGMEY LASQKCIHRDLAARNVLVTENNVMKIADFGLAR DINNIDYYKKTTNGRLPVKWMAPEALFDRVYTH QSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLL KEGHRMDKPANCTNELYMMMRDCWHAVPSQRPT FKQLVEDLDRILTLTTNEI*KFMASLRNWEKLV RRSGSCL*SQHFGRPRQADHEVRSSRPAWPTW*N PVSTKDTKN*PGVLVCTCNPSYSGRLRQESHLN RGGGGCSEPRSCHCIPALATERDSVSKKKK

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKSHQPNTKSLNQKCT WLRQGSR*RCAAC*KMPP*SVGLRMGCTWGPTI GQCLLGSTCR*RAPRLETPASMLVLPVGL*TVK LGTSW*MSQMPSHPEMMRMTPMVRKILSVRTVTT REHHTGPTQKRWKSGSMLCLRPTLSSFAAQPGG TQCQPCGG*KTGRSLSRSIALEATRYETSTGAS LWKVWSHLTREIIPV*WRMNTGPSITRTTWMLWS DRLTGPSSKPDCRQMPPQWSEET*SLSARFTVM PSPTSSGSSTWKRTAVNTGPTGCPTSRFSSTRG *IVPMQKCWLCSM*PRRMLGNIYVRSPII*GRPT SLPGSLSCQNSKRLEEKRRLQLPQTTWR*PFTA *GSS*SPVWW*QSSCAE*RTRPRSQTSAASRLC TS*PNVSPCGDR*QFRLSPAPP*TPTPRW*G*QH ASLQRQTPPCWQGSPSMNFQRTQNGSFQEIS*H WASPWEKVALGKWSWRKQWELTKTSPRRRSPWP *RC*KMMPQRKTFLIWCQRWR**R*LGNTRIS*I FLEPAHRMGLSMS*LSMPLKATSENTSEPGGHP GWSTPMTLTVFLRSR*PSRTWCHAPTSWPEAWS TWLPKNVFIEI*QPEMFW*QKTM**K*QTLDSPE ISTI*TITKRPPMGGFQSSGWLQKPCLIEYTLI RVMSGPSGC*CGRSSL*GARPTQGFPWRNFLSC *RKDTEWISQPTAPTNCT***GTVGMQCPPRDQR SSSW*KTWIEFSLSQPMRSESLWLH*ETGKSWS GAVAHACNPSTLGGRGRRIMRSGVPDQPGQHGE TLSLLKIQKISRACWCAPVIPATPGG*GRRVT*T GEAEVAVSRDHAIAFQPWRQSETPSQKKKK

Sequence FGFR2.dAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGAGCCACCAACCAAATACCAAATCTCTCAACCAGAAGTGTAC GTGGCTGCGCCAGGGGAGTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGGATGGGGTGCACTTGGGGCCCAACAATA GGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGGCCTCTATGCTTGTACTGCCAGTAGGACTGTAGACAGTGAAAC TTGGTACTTCATGGTGAATGTCACAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGTGCGGAAGATTTTGTCAGTGAGAACAGTAACAAC AAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGA ACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCAT TATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAG CGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATG CCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGGCCGCCGGTGT TAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAACTTTTGAGGACGCTGGGGAATATACGTGCTTGGCGGGTAATTCTATTGGGATA TCCTTTCACTCTGCATGGTTGACAGTTCTGCCAGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAG GGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAA GCTGACCAAACGTATCCCCCTGCGGAGACAGGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCT TCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGC CCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTT GAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTTCTTGGA GCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGT ACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTC CCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGATATCAAC AATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGAGTGATG TCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGG ACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAG TTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTCAGTCAGCCTCTCGAACCGTATTCACCTTGTTATCCTGACC CAAGATGAAATAAAACGTCTCTCTTCCCTTCTTTCAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACACAAGAAGT TCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAACGGCAGTGTTAAAACAT GAATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCTCCACTTGT ATATATGGATCAGAGGAGTAAATAATTGGAAAAGTAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGGAGAAGAAG GTTTCTGGAGCAGTGGACTGCCACAAGCCACCATGTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGACGTGCGTTC TGCCTTCCTTGTTAATTTTGTAATAATTGGAGAAGATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGCTGGATGTATGTAAATAT ATTCAAATTATGTATAAATATATATTATATATTTACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACCTAGAAAATTGGTCTCTC TTTTTTTAATAGCTATTTGCTAAATGCTGTTCTTACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGGAAAATGGT ATAACGTTAATTTATTAATAAATTGGTAATATACAAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCAGCACAGCA GACTAGTTAATCTATTGCTTGGACTTAACTAGTTATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGGAAAATGAAGTTTTGATT TATTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAGGTGTTTCATTTTGGTGT GCAACCCTGTCATTACGTCAACGCAACGTCTAACTGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCCTTGAGGAC AGACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAGCCTGTATTCTCTTCAGT GAATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACACAGAGTTGTTCTGCTGA TAGTTTTGGGGATACGTCCATCTTTTTAAGGGATTGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATACCTACATCAGACAAAAT ATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAATGCAGATTACACTGATC TTATGTGTTACAAAATTGGAGAAAGTATTTAATAAAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTAATGTTAACAAGACAAAA TAAATGTCACGCAACTT

FGFR2.F1 (forward primer)
FGFR2.R1 (reverse primer)
Single exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRATNQIPNLSTRSV RGCARGVARGALPVERCRRDQLD*GWGALGAQQ* DSAYWGVLADKGRHA*RLRPLCLYCQ*DCRQ*N LVLHGECHRCHLIRR**G*HRWCGRFCQ*EQ*Q QESTILDQHRKDGKAAPCCACGQHCQVSLPSRGE PNANHAVAEKREGV*AGASHWRLQGTKPALEPH YGKCGPI*QGKLYLCSGE*IRVHQSHVPPGCCG AIASPAHPPSRTAGKCLHSGRRRRRVCLQGLQ*C PAPHPVDQARGKERQ*IRARRAALPQGSQGRRC *HHGQRD*GSLYSECNF*GRWGIYVLGG*FYWD ILSLCMVDSSASAWKRKGDYSFPRLPGDSHLLHR GLLNRLYGGNSHPVPNEEHDQEARLQQPAGCAQ ADQTYPPAETGFG*VQLLHELQHPAGEDNNTPL FNGRHPHAGRGLRV*TSRGPKMGVSKR*ADTGQA PGRRLLWASGHGGSSGN*QRQAQGGGHRGREDV ER*CHRERPF*SGVRDGDDEDDWETQEYHKSSW SLHTGWASLCHS*VCL*RQPPRIPPSPEATRDGV LL*H*PCS*GADDLQGLGVMHLPAGQRHGVLGF PKMYSSRFSSQKCFGNRKQCDENSRLWTRQRYQ QYRLLQKDHQWAASSQVDGSRSPV**SIHSSE*C LVLRGVNVGDLHFRGLALPRDSRGGTF*AAEGR TQNG*ASQLHQRTVHDDEGLLACSALPETNVQA VGRRLGSNSHSHNQ*GILGPQSASRTVFTLLS*P KMK*NVSLPFFQEYLDLSQPLEQYSPSYPDTRS SCSSGDDSVFSPDPMPYEPCLPQYPHINGSVKT *MTVSACPQTGQHWEPSYTEQGDHASQSLLSPLV YMDQRSK*LEK*SAYV*RFIQLKTCNLPQEEKK VSGAVDCHKPPCNPSHLPCVLAVDQ*DSRWTCV LPSLLIL**LEKIYVSTHLQSTNAVYRCWMYVNI FKLCINIYYIFTRSYFLY*F*MDVPMHLENWSL FF**LFAKCCSYT*FLNFHRAEVEKYFCFQGKW YNVNLLINW*YTKQLIIYSFFCNLSGISMQAAQQ TS*SIAWT*LVIRSFEKRIFTIYD*FGENEVLI YLCLNAAVRRLFLDLLNAPY*KNSFIGRCFILV CNPVITSTQRLTGLPKINGTSVLLKDALIHSLRT DLS*NDSRMCFSLAAGLLLLSCTLIRLACILFS EF**WLPDSLALETPVRIFKSHHRKLKHRVVLL IVLGIRPSF*GIAFI*FWQDLTKRSSLIPTSDKI SPLFLLY*SIVFCFGNTHSLCNSRAR*MQITLI LCVTKLEKVFNKTC*FLY*Q*KCFYRY*C*QDK INVTQL

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEEPPTKYQISQPEVY VAAPGESLEVRCLLKDAAVISWTKDGVHLGPNN RTVLIGEYLQIKGATPRDSGLYACTASRTVDSET WYFMVNVTDAISSGDDEDDTDGAEDFVSENSNN KRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGG NPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLI MESVVPSDKGNYTCVVENEYGSINHTYHLDVVE RSPHRPILQAGLPANASTVVGGDVEFVCKVYSD AQPHIQWIKHVEKNGSKYGPDGLPYLKVLKAAGV NTTDKEIEVLYIRNVTFEDAGEYTCLAGNSIGI SFHSAWLTVLPAPGREKEITASPDYLEIAIYCI GVFLIACMVVTVILCRMKNTTKKPDFSSQPAVHK LTKRIPLRRQVSAESSSSMNSNTPLVRITTRLS STADTPMLAGVSEYELPEDPKWEFPRDKLTLGK PLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKML KDDATEKDLSDLVSEMEMMKMIGKHKNIINLLG ACTQDGPLYVIVEYASKGNLREYLRARRPPGME YSYDINRVPEEQMTFKDLVSCTYQLARGMEYLAS QKCIHRDLAARNVLVTENNVMKIADFGLARDIN NIDYYKKTTNGRLPVKWMAPEALFDRVYTHQSD VWSFGVLMWEIFTLGGSPYPGIPVEELFKLLKEG HRMDKPANCTNELYMMMRDCWHAVPSQRPTFKQ LVEDLDRILTLTTNEEYLDLSQPLEPYSPCYPD PR*NKTSLFPSFRNTWTSANLSNSIHLVTLTQEV LVLQEMILFFLQTPCLTNHAFLSIHT*TAVLKH E*LCLPVPKQDSTGNLATLSRETMPPRACCLHL YIWIRGVNNWKSNQHMCKDLYS*KLVIFPRRRRR FLEQWTATSHHVTPLTCRAYWLWTSRTQGGRAF CLPC*FCNNWRRFMSAHTYRAQMQYIGAGCM*I YSNYV*IYIIYLQGVIFCIDFKWMSQCT*KIGLS FFNSYLLNAVLTHNFLIFTEQRWKNTFAFRENG ITLIY**IGNIQNN*SFIVFFVI*VAFLCRQHS RLVNLLLGLN*LSDPLKREYLQYMTNLGKMKF*F ICV*MLLSDDCS*TS*MPHIKRTHS*EGVSFWC ATLSLRQRNV*LDFPR*MVPASS*KMP*SIP*G QTLVEMIAECASLWQLAFCF*VAH*SD*PVFSSV NFDNGFQTLWRWRRLLGSSSPIIEN*NTELFC* *FWGYVHLFKGLLSSNSGRTSPKDPASYLHQTK YRRCSFCTKVLCFALETPTHFAIAVQDECRLH*S YVLQNWRKYLIKPVNFYTDNKNVSTDINVNKTK *MSRN

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKSHQPNTKSLNQKCT WLRQGSR*RCAAC*KMPP*SVGLRMGCTWGPTI GQCLLGSTCR*RAPRLETPASMLVLPVGL*TVK LGTSW*MSQMPSHPEMMRMTPMVRKILSVRTVTT REHHTGPTQKRWKSGSMLCLRPTLSSFAAQPGG TQCQPCGG*KTGRSLSRSIALEATRYETSTGAS LWKVWSHLTREIIPV*WRMNTGPSITRTTWMLWS DRLTGPSSKPDCRQMPPQWSEET*SLSARFTVM PSPTSSGSSTWKRTAVNTGPTGCPTSRFSRPPV LTPRTKRLRFSIFGM*LLRTLGNIRAWRVILLGY PFTLHG*QFCQRLEEKRRLQLPQTTWR*PFTA* GSS*SPVWW*QSSCAE*RTRPRSQTSAASRLCT S*PNVSPCGDRFRLSPAPP*TPTPRW*G*QHASL QRQTPPCWQGSPSMNFQRTQNGSFQEIS*HWAS PWEKVALGKWSWRKQWELTKTSPRRRSPWP*RC *KMMPQRKTFLIWCQRWR**R*LGNTRIS*IFLE PAHRMGLSMS*LSMPLKATSENTSEPGGHPGWS TPMTLTVFLRSR*PSRTWCHAPTSWPEAWSTWL PKNVFIEI*QPEMFW*QKTM**K*QTLDSPEIST I*TITKRPPMGGFQSSGWLQKPCLIEYTLIRVM SGPSGC*CGRSSL*GARPTQGFPWRNFLSC*RK DTEWISQPTAPTNCT***GTVGMQCPPRDQRSSS W*KTWIEFSLSQPMRNTWTSVSLSNRIHLVILT QDEIKRLSSLLSGILGPQPTSRTVFT*LP*HKK FLFFRR*FCFFSRPHALRTMPSSVSTHKRQC*NM NDCVCLSPNRTALGT*LH*AGRPCLPELVVSTC IYGSEE*IIGKVISICVKIYTVENL*SSPGGEE GFWSSGLPQATM*PLSPAVRTGCGPVGLKVDVRS AFLVNFVIIGEDLCQHTLTEHKCSI*VLDVCKY IQIMYKYILYIYKELFFVLILNGCPNAPRKLVS LFLIAIC*MLFLHIIS*FSPSRGGKILLLSGKMV *R*FINKLVIYKTINHL*FFL*FKWHFYAGSTA D*LIYCLDLTSYQIL*KENIYNI*LIWGK*SFD LFVFKCCCQTIVLRPPKCPILKELIHRKVFHFGV QPCHYVNATSNWTSQDKWYQRPLKRCLNPFLED RP*LK**QNVLLSGSWPSASELHINQISLYSLQ *ILIMASRLFGVGDAC*DLQVPS*KIETQSCSAD SFGDTSIFLRDCFHLILAGPHQKIQPHTYIRQN IAVVPSVLKYCVLLWKHPLTLQ*PCKMNADYTD LMCYKIGESI**NLLIFILTIKMFLQILMLTRQN KCHAT

Sequence FGFR2.cAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGAGCCACCAACCAAATACCAAATCTCTCAACCAGAAGTGTAC GTGGCTGCGCCAGGGGAGTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGGATGGGGTGCACTTGGGGCCCAACAATA GGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGGCCTCTATGCTTGTACTGCCAGTAGGACTGTAGACAGTGAAAC TTGGTACTTCATGGTGAATGTCACAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGTGCGGAAGATTTTGTCAGTGAGAACAGTAACAAC AAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGA ACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCAT TATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAG CGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATG CCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGGCCGCCGGTGT TAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAACTTTTGAGGACGCTGGGGAATATACGTGCTTGGCGGGTAATTCTATTGGGATA TCCTTTCACTCTGCATGGTTGACAGTTCTGCCAGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAG GGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAA GCTGACCAAACGTATCCCCCTGCGGAGACAGGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGCCTCTCT TCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGGGCAAGC CCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAAGATGTT GAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTTCTTGGA GCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGATGGAGT ACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTTGGCTTC CCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGATATCAAC AATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGAGTGATG TCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAAGGAAGG ACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTCAAGCAG TTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTACCCTGACA CAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAACGGCAGTGT TAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGCTTGTTGTCT CCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAGTAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTCCCCAGGAGG AGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCATGTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTCAAGGTGGAC GTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAAGATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGCTGGATGTAT GTAAATATATTCAAATTATGTATAAATATATATTATATATTTACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACCTAGAAAATT GGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTTACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTGCTTTCAGGG AAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATACAAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCTATGCAGGCA GCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTTATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGGAAAATGAAG TTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAGGTGTTTCAT TTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAACTGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTAATCCATTCC TTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAGCCTGTATTC TCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACACAGAGTTGT TCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGATTGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATACCTACATCA GACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAATGCAGATTA CACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATAAAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTAATGTTAACA AGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F1 (forward primer)
FGFR2.R1 (reverse primer)
Single exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRATNQIPNLSTRSV RGCARGVARGALPVERCRRDQLD*GWGALGAQQ* DSAYWGVLADKGRHA*RLRPLCLYCQ*DCRQ*N LVLHGECHRCHLIRR**G*HRWCGRFCQ*EQ*Q QESTILDQHRKDGKAAPCCACGQHCQVSLPSRGE PNANHAVAEKREGV*AGASHWRLQGTKPALEPH YGKCGPI*QGKLYLCSGE*IRVHQSHVPPGCCG AIASPAHPPSRTAGKCLHSGRRRRRVCLQGLQ*C PAPHPVDQARGKERQ*IRARRAALPQGSQGRRC *HHGQRD*GSLYSECNF*GRWGIYVLGG*FYWD ILSLCMVDSSASAWKRKGDYSFPRLPGDSHLLHR GLLNRLYGGNSHPVPNEEHDQEARLQQPAGCAQ ADQTYPPAETGFG*VQLLHELQHPAGEDNNTPL FNGRHPHAGRGLRV*TSRGPKMGVSKR*ADTGQA PGRRLLWASGHGGSSGN*QRQAQGGGHRGREDV ER*CHRERPF*SGVRDGDDEDDWETQEYHKSSW SLHTGWASLCHS*VCL*RQPPRIPPSPEATRDGV LL*H*PCS*GADDLQGLGVMHLPAGQRHGVLGF PKMYSSRFSSQKCFGNRKQCDENSRLWTRQRYQ QYRLLQKDHQWAASSQVDGSRSPV**SIHSSE*C LVLRGVNVGDLHFRGLALPRDSRGGTF*AAEGR TQNG*ASQLHQRTVHDDEGLLACSALPETNVQA VGRRLGSNSHSHNQ*GILGPQPTSRTVFT*LP*H KKFLFFRR*FCFFSRPHALRTMPSSVSTHKRQC *NMNDCVCLSPNRTALGT*LH*AGRPCLPELVV STCIYGSEE*IIGKVISICVKIYTVENL*SSPGG EEGFWSSGLPQATM*PLSPAVRTGCGPVGLKVD VRSAFLVNFVIIGEDLCQHTLTEHKCSI*VLDV CKYIQIMYKYILYIYKELFFVLILNGCPNAPRKL VSLFLIAIC*MLFLHIIS*FSPSRGGKILLLSG KMV*R*FINKLVIYKTINHL*FFL*FKWHFYAG STAD*LIYCLDLTSYQIL*KENIYNI*LIWGK*S FDLFVFKCCCQTIVLRPPKCPILKELIHRKVFH FGVQPCHYVNATSNWTSQDKWYQRPLKRCLNPF LEDRP*LK**QNVLLSGSWPSASELHINQISLYS LQ*ILIMASRLFGVGDAC*DLQVPS*KIETQSC SADSFGDTSIFLRDCFHLILAGPHQKIQPHTYI RQNIAVVPSVLKYCVLLWKHPLTLQ*PCKMNADY TDLMCYKIGESI**NLLIFILTIKMFLQILMLT RQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEEPPTKYQISQPEVY VAAPGESLEVRCLLKDAAVISWTKDGVHLGPNN RTVLIGEYLQIKGATPRDSGLYACTASRTVDSET WYFMVNVTDAISSGDDEDDTDGAEDFVSENSNN KRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGG NPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLI MESVVPSDKGNYTCVVENEYGSINHTYHLDVVE RSPHRPILQAGLPANASTVVGGDVEFVCKVYSD AQPHIQWIKHVEKNGSKYGPDGLPYLKVLKAAGV NTTDKEIEVLYIRNVTFEDAGEYTCLAGNSIGI SFHSAWLTVLPAPGREKEITASPDYLEIAIYCI GVFLIACMVVTVILCRMKNTTKKPDFSSQPAVHK LTKRIPLRRQVSAESSSSMNSNTPLVRITTRLS STADTPMLAGVSEYELPEDPKWEFPRDKLTLGK PLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKML KDDATEKDLSDLVSEMEMMKMIGKHKNIINLLG ACTQDGPLYVIVEYASKGNLREYLRARRPPGME YSYDINRVPEEQMTFKDLVSCTYQLARGMEYLAS QKCIHRDLAARNVLVTENNVMKIADFGLARDIN NIDYYKKTTNGRLPVKWMAPEALFDRVYTHQSD VWSFGVLMWEIFTLGGSPYPGIPVEELFKLLKEG HRMDKPANCTNELYMMMRDCWHAVPSQRPTFKQ LVEDLDRILTLTTNEEYLDLSQPLEQYSPSYPD TRSSCSSGDDSVFSPDPMPYEPCLPQYPHINGSV KT*MTVSACPQTGQHWEPSYTEQGDHASQSLLS PLVYMDQRSK*LEK*SAYV*RFIQLKTCNLPQE EKKVSGAVDCHKPPCNPSHLPCVLAVDQ*DSRWT CVLPSLLIL**LEKIYVSTHLQSTNAVYRCWMY VNIFKLCINIYYIFTRSYFLY*F*MDVPMHLEN WSLFF**LFAKCCSYT*FLNFHRAEVEKYFCFQG KWYNVNLLINW*YTKQLIIYSFFCNLSGISMQA AQQTS*SIAWT*LVIRSFEKRIFTIYD*FGENE VLIYLCLNAAVRRLFLDLLNAPY*KNSFIGRCFI LVCNPVITSTQRLTGLPKINGTSVLLKDALIHS LRTDLS*NDSRMCFSLAAGLLLLSCTLIRLACI LFSEF**WLPDSLALETPVRIFKSHHRKLKHRVV LLIVLGIRPSF*GIAFI*FWQDLTKRSSLIPTS DKISPLFLLY*SIVFCFGNTHSLCNSRAR*MQI TLILCVTKLEKVFNKTC*FLY*Q*KCFYRY*C*Q DKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKSHQPNTKSLNQKCT WLRQGSR*RCAAC*KMPP*SVGLRMGCTWGPTI GQCLLGSTCR*RAPRLETPASMLVLPVGL*TVK LGTSW*MSQMPSHPEMMRMTPMVRKILSVRTVTT REHHTGPTQKRWKSGSMLCLRPTLSSFAAQPGG TQCQPCGG*KTGRSLSRSIALEATRYETSTGAS LWKVWSHLTREIIPV*WRMNTGPSITRTTWMLWS DRLTGPSSKPDCRQMPPQWSEET*SLSARFTVM PSPTSSGSSTWKRTAVNTGPTGCPTSRFSRPPV LTPRTKRLRFSIFGM*LLRTLGNIRAWRVILLGY PFTLHG*QFCQRLEEKRRLQLPQTTWR*PFTA* GSS*SPVWW*QSSCAE*RTRPRSQTSAASRLCT S*PNVSPCGDRFRLSPAPP*TPTPRW*G*QHASL QRQTPPCWQGSPSMNFQRTQNGSFQEIS*HWAS PWEKVALGKWSWRKQWELTKTSPRRRSPWP*RC *KMMPQRKTFLIWCQRWR**R*LGNTRIS*IFLE PAHRMGLSMS*LSMPLKATSENTSEPGGHPGWS TPMTLTVFLRSR*PSRTWCHAPTSWPEAWSTWL PKNVFIEI*QPEMFW*QKTM**K*QTLDSPEIST I*TITKRPPMGGFQSSGWLQKPCLIEYTLIRVM SGPSGC*CGRSSL*GARPTQGFPWRNFLSC*RK DTEWISQPTAPTNCT***GTVGMQCPPRDQRSSS W*KTWIEFSLSQPMRNTWTSANLSNSIHLVTLT QEVLVLQEMILFFLQTPCLTNHAFLSIHT*TAV LKHE*LCLPVPKQDSTGNLATLSRETMPPRACCL HLYIWIRGVNNWKSNQHMCKDLYS*KLVIFPRR RRRFLEQWTATSHHVTPLTCRAYWLWTSRTQGG RAFCLPC*FCNNWRRFMSAHTYRAQMQYIGAGCM *IYSNYV*IYIIYLQGVIFCIDFKWMSQCT*KI GLSFFNSYLLNAVLTHNFLIFTEQRWKNTFAFR ENGITLIY**IGNIQNN*SFIVFFVI*VAFLCRQ HSRLVNLLLGLN*LSDPLKREYLQYMTNLGKMK F*FICV*MLLSDDCS*TS*MPHIKRTHS*EGVS FWCATLSLRQRNV*LDFPR*MVPASS*KMP*SIP *GQTLVEMIAECASLWQLAFCF*VAH*SD*PVF SSVNFDNGFQTLWRWRRLLGSSSPIIEN*NTEL FC**FWGYVHLFKGLLSSNSGRTSPKDPASYLHQ TKYRRCSFCTKVLCFALETPTHFAIAVQDECRL H*SYVLQNWRKYLIKPVNFYTDNKNVSTDINVN KTK*MSRNLFF*

Sequence FGFR2.bAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGAGCCACCAACCAAATACCAAATCTCTCAACCAGAAGTGTAC GTGGCTGCGCCAGGGGAGTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGGATGGGGTGCACTTGGGGCCCAACAATA GGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGGCCTCTATGCTTGTACTGCCAGTAGGACTGTAGACAGTGAAAC TTGGTACTTCATGGTGAATGTCACAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGTGCGGAAGATTTTGTCAGTGAGAACAGTAACAAC AAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGA ACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCAT TATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAG CGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATG CCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGGCCGCCGGTGT TAACACCACGGACAAAGAGATTGAGGTTCTCTATATTCGGAATGTAACTTTTGAGGACGCTGGGGAATATACGTGCTTGGCGGGTAATTCTATTGGGATA TCCTTTCACTCTGCATGGTTGACAGTTCTGCCAGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCATAG GGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCACAA GCTGACCAAACGTATCCCCCTGCGGAGACAGGTAACAGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACACGC CTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACACTGG GCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGTGAA GATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAATCTT CTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCGGGA TGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTACTT GGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGAGAT ATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATCAGA GTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTGAA GGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACGTTC AAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTACC CTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAACGG CAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGCTTG TTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAGTAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTCCCC AGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCATGTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTCAAG GTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAAGATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGCTGG ATGTATGTAAATATATTCAAATTATGTATAAATATATATTATATATTTACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACCTAG AAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTTACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTGCTT TCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATACAAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCTATG CAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTTATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGGAAA ATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAGGTG TTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAACTGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTAATC CATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAGCCT GTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACACAG AGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGATTGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATACCT ACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAATGC AGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATAAAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTAATG TTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F1 (forward primer)
FGFR2.R1 (reverse primer)
Single exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRATNQIPNLSTRSV RGCARGVARGALPVERCRRDQLD*GWGALGAQQ* DSAYWGVLADKGRHA*RLRPLCLYCQ*DCRQ*N LVLHGECHRCHLIRR**G*HRWCGRFCQ*EQ*Q QESTILDQHRKDGKAAPCCACGQHCQVSLPSRGE PNANHAVAEKREGV*AGASHWRLQGTKPALEPH YGKCGPI*QGKLYLCSGE*IRVHQSHVPPGCCG AIASPAHPPSRTAGKCLHSGRRRRRVCLQGLQ*C PAPHPVDQARGKERQ*IRARRAALPQGSQGRRC *HHGQRD*GSLYSECNF*GRWGIYVLGG*FYWD ILSLCMVDSSASAWKRKGDYSFPRLPGDSHLLHR GLLNRLYGGNSHPVPNEEHDQEARLQQPAGCAQ ADQTYPPAETGNSFG*VQLLHELQHPAGEDNNT PLFNGRHPHAGRGLRV*TSRGPKMGVSKR*ADTG QAPGRRLLWASGHGGSSGN*QRQAQGGGHRGRE DVER*CHRERPF*SGVRDGDDEDDWETQEYHKS SWSLHTGWASLCHS*VCL*RQPPRIPPSPEATRD GVLL*H*PCS*GADDLQGLGVMHLPAGQRHGVL GFPKMYSSRFSSQKCFGNRKQCDENSRLWTRQR YQQYRLLQKDHQWAASSQVDGSRSPV**SIHSSE *CLVLRGVNVGDLHFRGLALPRDSRGGTF*AAE GRTQNG*ASQLHQRTVHDDEGLLACSALPETNV QAVGRRLGSNSHSHNQ*GILGPQPTSRTVFT*LP *HKKFLFFRR*FCFFSRPHALRTMPSSVSTHKR QC*NMNDCVCLSPNRTALGT*LH*AGRPCLPEL VVSTCIYGSEE*IIGKVISICVKIYTVENL*SSP GGEEGFWSSGLPQATM*PLSPAVRTGCGPVGLK VDVRSAFLVNFVIIGEDLCQHTLTEHKCSI*VL DVCKYIQIMYKYILYIYKELFFVLILNGCPNAPR KLVSLFLIAIC*MLFLHIIS*FSPSRGGKILLL SGKMV*R*FINKLVIYKTINHL*FFL*FKWHFY AGSTAD*LIYCLDLTSYQIL*KENIYNI*LIWGK *SFDLFVFKCCCQTIVLRPPKCPILKELIHRKV FHFGVQPCHYVNATSNWTSQDKWYQRPLKRCLN PFLEDRP*LK**QNVLLSGSWPSASELHINQISL YSLQ*ILIMASRLFGVGDAC*DLQVPS*KIETQ SCSADSFGDTSIFLRDCFHLILAGPHQKIQPHT YIRQNIAVVPSVLKYCVLLWKHPLTLQ*PCKMNA DYTDLMCYKIGESI**NLLIFILTIKMFLQILM LTRQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEEPPTKYQISQPEVY VAAPGESLEVRCLLKDAAVISWTKDGVHLGPNN RTVLIGEYLQIKGATPRDSGLYACTASRTVDSET WYFMVNVTDAISSGDDEDDTDGAEDFVSENSNN KRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGG NPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLI MESVVPSDKGNYTCVVENEYGSINHTYHLDVVE RSPHRPILQAGLPANASTVVGGDVEFVCKVYSD AQPHIQWIKHVEKNGSKYGPDGLPYLKVLKAAGV NTTDKEIEVLYIRNVTFEDAGEYTCLAGNSIGI SFHSAWLTVLPAPGREKEITASPDYLEIAIYCI GVFLIACMVVTVILCRMKNTTKKPDFSSQPAVHK LTKRIPLRRQVTVSAESSSSMNSNTPLVRITTR LSSTADTPMLAGVSEYELPEDPKWEFPRDKLTL GKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVK MLKDDATEKDLSDLVSEMEMMKMIGKHKNIINL LGACTQDGPLYVIVEYASKGNLREYLRARRPPG MEYSYDINRVPEEQMTFKDLVSCTYQLARGMEYL ASQKCIHRDLAARNVLVTENNVMKIADFGLARD INNIDYYKKTTNGRLPVKWMAPEALFDRVYTHQ SDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLLK EGHRMDKPANCTNELYMMMRDCWHAVPSQRPTF KQLVEDLDRILTLTTNEEYLDLSQPLEQYSPSY PDTRSSCSSGDDSVFSPDPMPYEPCLPQYPHING SVKT*MTVSACPQTGQHWEPSYTEQGDHASQSL LSPLVYMDQRSK*LEK*SAYV*RFIQLKTCNLP QEEKKVSGAVDCHKPPCNPSHLPCVLAVDQ*DSR WTCVLPSLLIL**LEKIYVSTHLQSTNAVYRCW MYVNIFKLCINIYYIFTRSYFLY*F*MDVPMHL ENWSLFF**LFAKCCSYT*FLNFHRAEVEKYFCF QGKWYNVNLLINW*YTKQLIIYSFFCNLSGISM QAAQQTS*SIAWT*LVIRSFEKRIFTIYD*FGE NEVLIYLCLNAAVRRLFLDLLNAPY*KNSFIGRC FILVCNPVITSTQRLTGLPKINGTSVLLKDALI HSLRTDLS*NDSRMCFSLAAGLLLLSCTLIRLA CILFSEF**WLPDSLALETPVRIFKSHHRKLKHR VVLLIVLGIRPSF*GIAFI*FWQDLTKRSSLIP TSDKISPLFLLY*SIVFCFGNTHSLCNSRAR*M QITLILCVTKLEKVFNKTC*FLY*Q*KCFYRY*C *QDKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKSHQPNTKSLNQKCT WLRQGSR*RCAAC*KMPP*SVGLRMGCTWGPTI GQCLLGSTCR*RAPRLETPASMLVLPVGL*TVK LGTSW*MSQMPSHPEMMRMTPMVRKILSVRTVTT REHHTGPTQKRWKSGSMLCLRPTLSSFAAQPGG TQCQPCGG*KTGRSLSRSIALEATRYETSTGAS LWKVWSHLTREIIPV*WRMNTGPSITRTTWMLWS DRLTGPSSKPDCRQMPPQWSEET*SLSARFTVM PSPTSSGSSTWKRTAVNTGPTGCPTSRFSRPPV LTPRTKRLRFSIFGM*LLRTLGNIRAWRVILLGY PFTLHG*QFCQRLEEKRRLQLPQTTWR*PFTA* GSS*SPVWW*QSSCAE*RTRPRSQTSAASRLCT S*PNVSPCGDR*QFRLSPAPP*TPTPRW*G*QHA SLQRQTPPCWQGSPSMNFQRTQNGSFQEIS*HW ASPWEKVALGKWSWRKQWELTKTSPRRRSPWP* RC*KMMPQRKTFLIWCQRWR**R*LGNTRIS*IF LEPAHRMGLSMS*LSMPLKATSENTSEPGGHPG WSTPMTLTVFLRSR*PSRTWCHAPTSWPEAWST WLPKNVFIEI*QPEMFW*QKTM**K*QTLDSPEI STI*TITKRPPMGGFQSSGWLQKPCLIEYTLIR VMSGPSGC*CGRSSL*GARPTQGFPWRNFLSC* RKDTEWISQPTAPTNCT***GTVGMQCPPRDQRS SSW*KTWIEFSLSQPMRNTWTSANLSNSIHLVT LTQEVLVLQEMILFFLQTPCLTNHAFLSIHT*T AVLKHE*LCLPVPKQDSTGNLATLSRETMPPRAC CLHLYIWIRGVNNWKSNQHMCKDLYS*KLVIFP RRRRRFLEQWTATSHHVTPLTCRAYWLWTSRTQ GGRAFCLPC*FCNNWRRFMSAHTYRAQMQYIGAG CM*IYSNYV*IYIIYLQGVIFCIDFKWMSQCT* KIGLSFFNSYLLNAVLTHNFLIFTEQRWKNTFA FRENGITLIY**IGNIQNN*SFIVFFVI*VAFLC RQHSRLVNLLLGLN*LSDPLKREYLQYMTNLGK MKF*FICV*MLLSDDCS*TS*MPHIKRTHS*EG VSFWCATLSLRQRNV*LDFPR*MVPASS*KMP*S IP*GQTLVEMIAECASLWQLAFCF*VAH*SD*P VFSSVNFDNGFQTLWRWRRLLGSSSPIIEN*NT ELFC**FWGYVHLFKGLLSSNSGRTSPKDPASYL HQTKYRRCSFCTKVLCFALETPTHFAIAVQDEC RLH*SYVLQNWRKYLIKPVNFYTDNKNVSTDIN VNKTK*MSRNLFF*

Sequence FGFR2.aAug05

CCTACGGCCGCATCGCGGCCGAGCCCTCGCCTCCCGGGCCGGTGTCTCCGGCTGCTCGGAGGCGTCGGGAGCCGCGCGGGACTCGGGGCGGCCCGGGCGC GCGGCGCTGATTGGCAGAGAGGGCGCCGCCGTCCAGGAAACGGCTCGGGTTTCAGTGGGGGCGTGACCCGCCGCGAGGAGGCGGGGGCGGCGGCGGCGGC GCGGGCGGCGGCTGGAGGAGAGCGCGGTGGAGAGCCGAGCGGGCGGGCGGCGGGTGCGGAGCGGGCGAGGGAGCGCGCGCGGCCGCCACAAAGCTCGGGC GCCGCGGGGCTGCATGCGGCGTACCTGGCCCGGCGCGGCGACTGCTCTCCGGGCTGGCGGGGGCCGGCCGCGAGCCCCGGGGGCCCCGAGGCCGCAGCTT GCCTGCGCGCTCTGAGCCTTCGCAACTCGCGAGCAAAGTTTGGTGGAGGCAACGCCAAGCCTGAGTCCTTTCTTCCTCTCGTTCCCCAAATCCGAGGGCA GCCCGCGGGCGTCATGCCCGCGCTCCTCCGCAGCCTGGGGTACGCGTGAAGCCCGGGAGGCTTGGCGCCGGCGAAGACCCAAGGACCACTCTTCTGCGTT TGGAGTTGCTCCCCGCAACCCCGGGCTCGTCGCTTTCTCCATCCCGACCCACGCGGGGCGCGGGGACAACACAGGTCGCGGAGGAGCGTTGCCATTCAAG TGACTGCAGCAGCAGCGGCAGCGCCTCGGTTCCTGAGCCCACCGCAGGCTGAAGGCATTGCGCGTAGTCCATGCCCGTAGAGGAAGTGTGCAGATGGGAT TAACGTCCACATGGAGATATGGAAGAGGACCGGGGATTGGTACCGTAACCATGGTCAGCTGGGGTCGTTTCATCTGCCTGGTCGTGGTCACCATGGCAAC CTTGTCCCTGGCCCGGCCCTCCTTCAGTTTAGTTGAGGATACCACATTAGAGCCAGAAGAGCCACCAACCAAATACCAAATCTCTCAACCAGAAGTGTAC GTGGCTGCGCCAGGGGAGTCGCTAGAGGTGCGCTGCCTGTTGAAAGATGCCGCCGTGATCAGTTGGACTAAGGATGGGGTGCACTTGGGGCCCAACAATA GGACAGTGCTTATTGGGGAGTACTTGCAGATAAAGGGCGCCACGCCTAGAGACTCCGGCCTCTATGCTTGTACTGCCAGTAGGACTGTAGACAGTGAAAC TTGGTACTTCATGGTGAATGTCACAGATGCCATCTCATCCGGAGATGATGAGGATGACACCGATGGTGCGGAAGATTTTGTCAGTGAGAACAGTAACAAC AAGAGAGCACCATACTGGACCAACACAGAAAAGATGGAAAAGCGGCTCCATGCTGTGCCTGCGGCCAACACTGTCAAGTTTCGCTGCCCAGCCGGGGGGA ACCCAATGCCAACCATGCGGTGGCTGAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTACAAGGTACGAAACCAGCACTGGAGCCTCAT TATGGAAAGTGTGGTCCCATCTGACAAGGGAAATTATACCTGTGTAGTGGAGAATGAATACGGGTCCATCAATCACACGTACCACCTGGATGTTGTGGAG CGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGCAAATGCCTCCACAGTGGTCGGAGGAGACGTAGAGTTTGTCTGCAAGGTTTACAGTGATG CCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCCGACGGGCTGCCCTACCTCAAGGTTCTCAAGCACTCGGGGAT AAATAGTTCCAATGCAGAAGTGCTGGCTCTGTTCAATGTGACCGAGGCGGATGCTGGGGAATATATATGTAAGGTCTCCAATTATATAGGGCAGGCCAAC CAGTCTGCCTGGCTCACTGTCCTGCCAAAACAGCAAGCGCCTGGAAGAGAAAAGGAGATTACAGCTTCCCCAGACTACCTGGAGATAGCCATTTACTGCA TAGGGGTCTTCTTAATCGCCTGTATGGTGGTAACAGTCATCCTGTGCCGAATGAAGAACACGACCAAGAAGCCAGACTTCAGCAGCCAGCCGGCTGTGCA CAAGCTGACCAAACGTATCCCCCTGCGGAGACAGGTAACAGTTTCGGCTGAGTCCAGCTCCTCCATGAACTCCAACACCCCGCTGGTGAGGATAACAACA CGCCTCTCTTCAACGGCAGACACCCCCATGCTGGCAGGGGTCTCCGAGTATGAACTTCCAGAGGACCCAAAATGGGAGTTTCCAAGAGATAAGCTGACAC TGGGCAAGCCCCTGGGAGAAGGTTGCTTTGGGCAAGTGGTCATGGCGGAAGCAGTGGGAATTGACAAAGACAAGCCCAAGGAGGCGGTCACCGTGGCCGT GAAGATGTTGAAAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGATGGAGATGATGAAGATGATTGGGAAACACAAGAATATCATAAAT CTTCTTGGAGCCTGCACACAGGATGGGCCTCTCTATGTCATAGTTGAGTATGCCTCTAAAGGCAACCTCCGAGAATACCTCCGAGCCCGGAGGCCACCCG GGATGGAGTACTCCTATGACATTAACCGTGTTCCTGAGGAGCAGATGACCTTCAAGGACTTGGTGTCATGCACCTACCAGCTGGCCAGAGGCATGGAGTA CTTGGCTTCCCAAAAATGTATTCATCGAGATTTAGCAGCCAGAAATGTTTTGGTAACAGAAAACAATGTGATGAAAATAGCAGACTTTGGACTCGCCAGA GATATCAACAATATAGACTATTACAAAAAGACCACCAATGGGCGGCTTCCAGTCAAGTGGATGGCTCCAGAAGCCCTGTTTGATAGAGTATACACTCATC AGAGTGATGTCTGGTCCTTCGGGGTGTTAATGTGGGAGATCTTCACTTTAGGGGGCTCGCCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCT GAAGGAAGGACACAGAATGGATAAGCCAGCCAACTGCACCAACGAACTGTACATGATGATGAGGGACTGTTGGCATGCAGTGCCCTCCCAGAGACCAACG TTCAAGCAGTTGGTAGAAGACTTGGATCGAATTCTCACTCTCACAACCAATGAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTT ACCCTGACACAAGAAGTTCTTGTTCTTCAGGAGATGATTCTGTTTTTTCTCCAGACCCCATGCCTTACGAACCATGCCTTCCTCAGTATCCACACATAAA CGGCAGTGTTAAAACATGAATGACTGTGTCTGCCTGTCCCCAAACAGGACAGCACTGGGAACCTAGCTACACTGAGCAGGGAGACCATGCCTCCCAGAGC TTGTTGTCTCCACTTGTATATATGGATCAGAGGAGTAAATAATTGGAAAAGTAATCAGCATATGTGTAAAGATTTATACAGTTGAAAACTTGTAATCTTC CCCAGGAGGAGAAGAAGGTTTCTGGAGCAGTGGACTGCCACAAGCCACCATGTAACCCCTCTCACCTGCCGTGCGTACTGGCTGTGGACCAGTAGGACTC AAGGTGGACGTGCGTTCTGCCTTCCTTGTTAATTTTGTAATAATTGGAGAAGATTTATGTCAGCACACACTTACAGAGCACAAATGCAGTATATAGGTGC TGGATGTATGTAAATATATTCAAATTATGTATAAATATATATTATATATTTACAAGGAGTTATTTTTTGTATTGATTTTAAATGGATGTCCCAATGCACC TAGAAAATTGGTCTCTCTTTTTTTAATAGCTATTTGCTAAATGCTGTTCTTACACATAATTTCTTAATTTTCACCGAGCAGAGGTGGAAAAATACTTTTG CTTTCAGGGAAAATGGTATAACGTTAATTTATTAATAAATTGGTAATATACAAAACAATTAATCATTTATAGTTTTTTTTGTAATTTAAGTGGCATTTCT ATGCAGGCAGCACAGCAGACTAGTTAATCTATTGCTTGGACTTAACTAGTTATCAGATCCTTTGAAAAGAGAATATTTACAATATATGACTAATTTGGGG AAAATGAAGTTTTGATTTATTTGTGTTTAAATGCTGCTGTCAGACGATTGTTCTTAGACCTCCTAAATGCCCCATATTAAAAGAACTCATTCATAGGAAG GTGTTTCATTTTGGTGTGCAACCCTGTCATTACGTCAACGCAACGTCTAACTGGACTTCCCAAGATAAATGGTACCAGCGTCCTCTTAAAAGATGCCTTA ATCCATTCCTTGAGGACAGACCTTAGTTGAAATGATAGCAGAATGTGCTTCTCTCTGGCAGCTGGCCTTCTGCTTCTGAGTTGCACATTAATCAGATTAG CCTGTATTCTCTTCAGTGAATTTTGATAATGGCTTCCAGACTCTTTGGCGTTGGAGACGCCTGTTAGGATCTTCAAGTCCCATCATAGAAAATTGAAACA CAGAGTTGTTCTGCTGATAGTTTTGGGGATACGTCCATCTTTTTAAGGGATTGCTTTCATCTAATTCTGGCAGGACCTCACCAAAAGATCCAGCCTCATA CCTACATCAGACAAAATATCGCCGTTGTTCCTTCTGTACTAAAGTATTGTGTTTTGCTTTGGAAACACCCACTCACTTTGCAATAGCCGTGCAAGATGAA TGCAGATTACACTGATCTTATGTGTTACAAAATTGGAGAAAGTATTTAATAAAACCTGTTAATTTTTATACTGACAATAAAAATGTTTCTACAGATATTA ATGTTAACAAGACAAAATAAATGTCACGCAACTTATTTTTTTAA

FGFR2.F1 (forward primer)
FGFR2.R1 (reverse primer)
Single exon skipping

Frame 0

PTAASRPSPRLPGRCLRLLGGVGSRAGLGAARA RGADWQRGRRRPGNGSGFSGGVTRREEAGAAAAA RAAAGGERGGEPSGRAAGAERARERARPPQSSG AAGLHAAYLARRGDCSPGWRGPAASPGGPEAAA CLRALSLRNSRAKFGGGNAKPESFLPLVPQIRGQ PAGVMPALLRSLGYA*SPGGLAPAKTQGPLFCV WSCSPQPRARRFLHPDPRGARGQHRSRRSVAIQ VTAAAAAAPRFLSPPQAEGIARSPCP*RKCADGI NVHMEIWKRTGDWYRNHGQLGSFHLPGRGHHGN LVPGPALLQFS*GYHIRARRATNQIPNLSTRSV RGCARGVARGALPVERCRRDQLD*GWGALGAQQ* DSAYWGVLADKGRHA*RLRPLCLYCQ*DCRQ*N LVLHGECHRCHLIRR**G*HRWCGRFCQ*EQ*Q QESTILDQHRKDGKAAPCCACGQHCQVSLPSRGE PNANHAVAEKREGV*AGASHWRLQGTKPALEPH YGKCGPI*QGKLYLCSGE*IRVHQSHVPPGCCG AIASPAHPPSRTAGKCLHSGRRRRRVCLQGLQ*C PAPHPVDQARGKERQ*IRARRAALPQGSQALGD K*FQCRSAGSVQCDRGGCWGIYM*GLQLYRAGQ PVCLAHCPAKTASAWKRKGDYSFPRLPGDSHLLH RGLLNRLYGGNSHPVPNEEHDQEARLQQPAGCA QADQTYPPAETGNSFG*VQLLHELQHPAGEDNN TPLFNGRHPHAGRGLRV*TSRGPKMGVSKR*ADT GQAPGRRLLWASGHGGSSGN*QRQAQGGGHRGR EDVER*CHRERPF*SGVRDGDDEDDWETQEYHK SSWSLHTGWASLCHS*VCL*RQPPRIPPSPEATR DGVLL*H*PCS*GADDLQGLGVMHLPAGQRHGV LGFPKMYSSRFSSQKCFGNRKQCDENSRLWTRQ RYQQYRLLQKDHQWAASSQVDGSRSPV**SIHSS E*CLVLRGVNVGDLHFRGLALPRDSRGGTF*AA EGRTQNG*ASQLHQRTVHDDEGLLACSALPETN VQAVGRRLGSNSHSHNQ*GILGPQPTSRTVFT*L P*HKKFLFFRR*FCFFSRPHALRTMPSSVSTHK RQC*NMNDCVCLSPNRTALGT*LH*AGRPCLPE LVVSTCIYGSEE*IIGKVISICVKIYTVENL*SS PGGEEGFWSSGLPQATM*PLSPAVRTGCGPVGL KVDVRSAFLVNFVIIGEDLCQHTLTEHKCSI*V LDVCKYIQIMYKYILYIYKELFFVLILNGCPNAP RKLVSLFLIAIC*MLFLHIIS*FSPSRGGKILL LSGKMV*R*FINKLVIYKTINHL*FFL*FKWHF YAGSTAD*LIYCLDLTSYQIL*KENIYNI*LIWG K*SFDLFVFKCCCQTIVLRPPKCPILKELIHRK VFHFGVQPCHYVNATSNWTSQDKWYQRPLKRCL NPFLEDRP*LK**QNVLLSGSWPSASELHINQIS LYSLQ*ILIMASRLFGVGDAC*DLQVPS*KIET QSCSADSFGDTSIFLRDCFHLILAGPHQKIQPH TYIRQNIAVVPSVLKYCVLLWKHPLTLQ*PCKMN ADYTDLMCYKIGESI**NLLIFILTIKMFLQIL MLTRQNKCHATYFF

Frame 1

LRPHRGRALASRAGVSGCSEASGAARDSGRPGR AALIGREGAAVQETARVSVGA*PAARRRGRRRR RGRRLEESAVESRAGGRRVRSGRGSARGRHKARA PRGCMRRTWPGAATALRAGGGRPRAPGAPRPQL ACAL*AFATREQSLVEATPSLSPFFLSFPKSEG SPRASCPRSSAAWGTREAREAWRRRRPKDHSSAF GVAPRNPGLVAFSIPTHAGRGDNTGRGGALPFK *LQQQRQRLGS*AHRRLKALRVVHARRGSVQMG LTSTWRYGRGPGIGTVTMVSWGRFICLVVVTMAT LSLARPSFSLVEDTTLEPEEPPTKYQISQPEVY VAAPGESLEVRCLLKDAAVISWTKDGVHLGPNN RTVLIGEYLQIKGATPRDSGLYACTASRTVDSET WYFMVNVTDAISSGDDEDDTDGAEDFVSENSNN KRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGG NPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLI MESVVPSDKGNYTCVVENEYGSINHTYHLDVVE RSPHRPILQAGLPANASTVVGGDVEFVCKVYSD AQPHIQWIKHVEKNGSKYGPDGLPYLKVLKHSGI NSSNAEVLALFNVTEADAGEYICKVSNYIGQAN QSAWLTVLPKQQAPGREKEITASPDYLEIAIYC IGVFLIACMVVTVILCRMKNTTKKPDFSSQPAVH KLTKRIPLRRQVTVSAESSSSMNSNTPLVRITT RLSSTADTPMLAGVSEYELPEDPKWEFPRDKLT LGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAV KMLKDDATEKDLSDLVSEMEMMKMIGKHKNIIN LLGACTQDGPLYVIVEYASKGNLREYLRARRPP GMEYSYDINRVPEEQMTFKDLVSCTYQLARGMEY LASQKCIHRDLAARNVLVTENNVMKIADFGLAR DINNIDYYKKTTNGRLPVKWMAPEALFDRVYTH QSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLL KEGHRMDKPANCTNELYMMMRDCWHAVPSQRPT FKQLVEDLDRILTLTTNEEYLDLSQPLEQYSPS YPDTRSSCSSGDDSVFSPDPMPYEPCLPQYPHIN GSVKT*MTVSACPQTGQHWEPSYTEQGDHASQS LLSPLVYMDQRSK*LEK*SAYV*RFIQLKTCNL PQEEKKVSGAVDCHKPPCNPSHLPCVLAVDQ*DS RWTCVLPSLLIL**LEKIYVSTHLQSTNAVYRC WMYVNIFKLCINIYYIFTRSYFLY*F*MDVPMH LENWSLFF**LFAKCCSYT*FLNFHRAEVEKYFC FQGKWYNVNLLINW*YTKQLIIYSFFCNLSGIS MQAAQQTS*SIAWT*LVIRSFEKRIFTIYD*FG ENEVLIYLCLNAAVRRLFLDLLNAPY*KNSFIGR CFILVCNPVITSTQRLTGLPKINGTSVLLKDAL IHSLRTDLS*NDSRMCFSLAAGLLLLSCTLIRL ACILFSEF**WLPDSLALETPVRIFKSHHRKLKH RVVLLIVLGIRPSF*GIAFI*FWQDLTKRSSLI PTSDKISPLFLLY*SIVFCFGNTHSLCNSRAR* MQITLILCVTKLEKVFNKTC*FLY*Q*KCFYRY* C*QDKINVTQLIFL

Frame 2

YGRIAAEPSPPGPVSPAARRRREPRGTRGGPGA RR*LAERAPPSRKRLGFQWGRDPPRGGGGGGGG AGGGWRRARWRAERAGGGCGAGEGARAAATKLG RRGAACGVPGPARRLLSGLAGAGREPRGPRGRSL PARSEPSQLASKVWWRQRQA*VLSSSRSPNPRA ARGRHARAPPQPGVRVKPGRLGAGEDPRTTLLR LELLPATPGSSLSPSRPTRGAGTTQVAEERCHSS DCSSSGSASVPEPTAG*RHCA*SMPVEEVCRWD *RPHGDMEEDRGLVP*PWSAGVVSSAWSWSPWQ PCPWPGPPSV*LRIPH*SQKSHQPNTKSLNQKCT WLRQGSR*RCAAC*KMPP*SVGLRMGCTWGPTI GQCLLGSTCR*RAPRLETPASMLVLPVGL*TVK LGTSW*MSQMPSHPEMMRMTPMVRKILSVRTVTT REHHTGPTQKRWKSGSMLCLRPTLSSFAAQPGG TQCQPCGG*KTGRSLSRSIALEATRYETSTGAS LWKVWSHLTREIIPV*WRMNTGPSITRTTWMLWS DRLTGPSSKPDCRQMPPQWSEET*SLSARFTVM PSPTSSGSSTWKRTAVNTGPTGCPTSRFSSTRG *IVPMQKCWLCSM*PRRMLGNIYVRSPII*GRPT SLPGSLSCQNSKRLEEKRRLQLPQTTWR*PFTA *GSS*SPVWW*QSSCAE*RTRPRSQTSAASRLC TS*PNVSPCGDR*QFRLSPAPP*TPTPRW*G*QH ASLQRQTPPCWQGSPSMNFQRTQNGSFQEIS*H WASPWEKVALGKWSWRKQWELTKTSPRRRSPWP *RC*KMMPQRKTFLIWCQRWR**R*LGNTRIS*I FLEPAHRMGLSMS*LSMPLKATSENTSEPGGHP GWSTPMTLTVFLRSR*PSRTWCHAPTSWPEAWS TWLPKNVFIEI*QPEMFW*QKTM**K*QTLDSPE ISTI*TITKRPPMGGFQSSGWLQKPCLIEYTLI RVMSGPSGC*CGRSSL*GARPTQGFPWRNFLSC *RKDTEWISQPTAPTNCT***GTVGMQCPPRDQR SSSW*KTWIEFSLSQPMRNTWTSANLSNSIHLV TLTQEVLVLQEMILFFLQTPCLTNHAFLSIHT* TAVLKHE*LCLPVPKQDSTGNLATLSRETMPPRA CCLHLYIWIRGVNNWKSNQHMCKDLYS*KLVIF PRRRRRFLEQWTATSHHVTPLTCRAYWLWTSRT QGGRAFCLPC*FCNNWRRFMSAHTYRAQMQYIGA GCM*IYSNYV*IYIIYLQGVIFCIDFKWMSQCT *KIGLSFFNSYLLNAVLTHNFLIFTEQRWKNTF AFRENGITLIY**IGNIQNN*SFIVFFVI*VAFL CRQHSRLVNLLLGLN*LSDPLKREYLQYMTNLG KMKF*FICV*MLLSDDCS*TS*MPHIKRTHS*E GVSFWCATLSLRQRNV*LDFPR*MVPASS*KMP* SIP*GQTLVEMIAECASLWQLAFCF*VAH*SD* PVFSSVNFDNGFQTLWRWRRLLGSSSPIIEN*N TELFC**FWGYVHLFKGLLSSNSGRTSPKDPASY LHQTKYRRCSFCTKVLCFALETPTHFAIAVQDE CRLH*SYVLQNWRKYLIKPVNFYTDNKNVSTDI NVNKTK*MSRNLFF*