Back to Rnomics Platform
 Open Session
×
1 warning:
  • Access restricted to public data.
    Please sign in at Rnomics Platform to access your data.
Navigation
Home
Sequences

Sequence FGFR1.nAug05

CCTCCAACTCGCGCTCGGGAGCTGGCGAGGCGGCGGCGGCTCCTCAGGTCAGTTTGAAAAGGAGGATCGAGCTCACTGTGGAGTATCCATGGAGATGTGG
AGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGAGCTGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGCCACACTCTGCACCGCTAGG
CCGTCCCCGACCTTGCCTGAACAAGATGCTCTCCCCTCCTCGGAGGATGATGATGATGATGATGACTCCTCTTCAGAGGAGAAAGAAACAGATAACACCA
AACCAAACCGTATGCCCGTAGCTCCATATTGGACATCCCCAGAAAAGATGGAAAAGAAATTGCATGCAGTGCCGGCTGCCAAGACAGTGAAGTTCAAATG
CCCTTCCAGTGGGACCCCAAACCCCACACTGCGCTGGTTGAAAAATGGCAAAGAATTCAAACCTGACCACAGAATTGGAGGCTACAAGAGCGGTCCCCTC
ACCGGCCCATCCTGCAAGCAGGGTTGCCCGCCAACAAAACAGTGGCCCTGGGTAGCAACGTGGAGTTCATGTGTAAGGTGTACAGTGACCCGCAGCCGCA
CATCCAGTGGCTAAAGCACATCGAGGTGAATGGGAGCAAGATTGGCCCAGACAACCTGCCTTATGTCCAGATCTTGAAGGTAATCATGGCACCAGTCTTC
GTGGGCCAGTCTACTGGGAAGGAGACCACTGTCTCGGGGGCTCAAGTTCCTGTGGGCAGGCTCAGTTGCCCCCGAATGGGATCATTCCTCACGCTTCAGG
CACACACACTCCATCTCAGTAGGGATCTAGCCACATCCCCCAGGACTAGTAACAGAGGTCACAAAGTGGAGGTGAGCTGGGAACAGAGGGCTGCAGGGAT
GGGTGGTGCTGGTCTGTAATAAGCTTTGAGAGCAACGTCACTGGGGCTTTGGGGTCAGCTACACAAGGAAGGCATTTGGACCCCTGCCTTTTCATTGCCC
GAAACCAGAGCCTTTCCACCAAGCGTTTCCCAGTCTTAGCCCTGTGTCCTGAGTTACGTACGATCTTTCTGGCAAATGGGGTGCATGATAAGAGCATCTC
TTACGAAGAGTTGGAAAAACAAATGCCATATATAAATTCTAAGCCATATGAGGACGAGGAGTAATGGCATTTTCTTCCTTTTTCCTCTCACTCCCAGACA
TTCATTGTCCCTGAATGCTCCATTAATCCAGGGAAGGTAATTGCCTAAATCTCCAGTGGATCTCGCAACAGGAAGGAACCAGAAGCTGGGAAAGTTGTTT
ACCTCTTTGTCCCAGAGTTAGACCTCATCCTCCCCTAGCTTAGCTGTCTCAGAGATATACTGGCCCTCCCTTCTCTTCTCTTTGCTGCTGGTGCTAAAAC
TGCTCTGTAGGTCATTGGCCACTGTCTCCACTCACAACCCCTGCTCCAGTCCTGGAGGGAGTGGGTTAAACACAAATAGAACATTCCATTTGAAGCAGTG
ATTCTTTTTTTTTTTTTTTTTTTTTAATCAAATGCTTTGGACTTTTGAAGTCCACTTGTTCTGTACTTGTAAAAGGGAAAGAAGGCCGGGCGCAGTGGCT
CACGCCTGTAATCCCAGCACTTTAGATCACTTGAGGTCAGGAGTTTGAGACCAGCCCGGCCAACATGGTGAAACCCCATCTCTACTAAAAATACAAAAAT
TAGCTGTGCATAGTGGTTGGCACCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAAGCTAACTGCTTGAACCCAGAAGGCAGAGGTTGCAGTGAGCTGAG
ATCACGCCACTGCACTCCAGCCTGGGTGACAGAGTGAGTGAGACTCTGCGTT
  • FGFR1.F11 (forward primer)
  • FGFR1.R11 (reverse primer)
  • Single exon skipping

Frame 0

PPTRARELARRRRLLRSV*KGGSSSLWSIHGDV
EPCHQPLTAELGCGAGSASSSGLCWSQPHSAPLG
RPRPCLNKMLSPPRRMMMMMMTPLQRRKKQITP
NQTVCP*LHIGHPQKRWKRNCMQCRLPRQ*SSN
ALPVGPQTPHCAG*KMAKNSNLTTELEATRAVPS
PAHPASRVARQQNSGPG*QRGVHV*GVQ*PAAA
HPVAKAHRGEWEQDWPRQPALCPDLEGNHGTSL
RGPVYWEGDHCLGGSSSCGQAQLPPNGIIPHASG
THTPSQ*GSSHIPQD**QRSQSGGELGTEGCRD
GWCWSVISFESNVTGALGSATQGRHLDPCLFIA
RNQSLSTKRFPVLALCPELRTIFLANGVHDKSIS
YEELEKQMPYINSKPYEDEE*WHFLPFSSHSQT
FIVPECSINPGKVIA*ISSGSRNRKEPEAGKVV
YLFVPELDLILP*LSCLRDILALPSLLFAAGAKT
AL*VIGHCLHSQPLLQSWREWVKHK*NIPFEAV
ILFFFFFF*SNALDF*SPLVLYL*KGKKAGRSG
SRL*SQHFRSLEVRSLRPARPTW*NPISTKNTKI
SCA*WLAPVVPATQEAEAS*LLEPRRQRLQ*AE
ITPLHSSLGDRVSETLR

Frame 1

LQLALGSWRGGGGSSGQFEKEDRAHCGVSMEMW
SLVTNL*LQNWDVELEVPPLLGCAGHSHTLHR*
AVPDLA*TRCSPLLGG******LLFRGERNR*HQ
TKPYARSSILDIPRKDGKEIACSAGCQDSEVQM
PFQWDPKPHTALVEKWQRIQT*PQNWRLQERSP
HRPILQAGLPANKTVALGSNVEFMCKVYSDPQPH
IQWLKHIEVNGSKIGPDNLPYVQILKVIMAPVF
VGQSTGKETTVSGAQVPVGRLSCPRMGSFLTLQ
AHTLHLSRDLATSPRTSNRGHKVEVSWEQRAAGM
GGAGL**ALRATSLGLWGQLHKEGIWTPAFSLP
ETRAFPPSVSQS*PCVLSYVRSFWQMGCMIRAS
LTKSWKNKCHI*ILSHMRTRSNGIFFLFPLTPRH
SLSLNAPLIQGR*LPKSPVDLATGRNQKLGKLF
TSLSQS*TSSSPSLAVSEIYWPSLLFSLLLVLK
LLCRSLATVSTHNPCSSPGGSGLNTNRTFHLKQ*
FFFFFFFFNQMLWTFEVHLFCTCKRERRPGAVA
HACNPSTLDHLRSGV*DQPGQHGETPSLLKIQK
LAVHSGWHL*SQLLRRLRQANCLNPEGRGCSELR
SRHCTPAWVTE*VRLCV

Frame 2

SNSRSGAGEAAAAPQVSLKRRIELTVEYPWRCG
ALSPTSNCRTGMWSWKCLLFWAVLVTATLCTAR
PSPTLPEQDALPSSEDDDDDDDSSSEEKETDNT
KPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKC
PSSGTPNPTLRWLKNGKEFKPDHRIGGYKSGPL
TGPSCKQGCPPTKQWPWVATWSSCVRCTVTRSR
TSSG*STSR*MGARLAQTTCLMSRS*R*SWHQSS
WASLLGRRPLSRGLKFLWAGSVAPEWDHSSRFR
HTHSISVGI*PHPPGLVTEVTKWR*AGNRGLQG
WVVLVCNKL*EQRHWGFGVSYTRKAFGPLPFHCP
KPEPFHQAFPSLSPVS*VTYDLSGKWGA**EHL
LRRVGKTNAIYKF*AI*GRGVMAFSSFFLSLPD
IHCP*MLH*SREGNCLNLQWISQQEGTRSWESCL
PLCPRVRPHPPLA*LSQRYTGPPFSSLCCWC*N
CSVGHWPLSPLTTPAPVLEGVG*TQIEHSI*SS
DSFFFFFFLIKCFGLLKSTCSVLVKGKEGRAQWL
TPVIPAL*IT*GQEFETSPANMVKPHLY*KYKN
*LCIVVGTCSPSYSGG*GKLTA*TQKAEVAVS*
DHATALQPG*QSE*DSA

Sequence FGFR1.kAug05

CCCCGCCGGCGCATAGCGCTCGGAGCGCTCTTGCGGCCACAGGCGCGGCGTCCTCGGCGGCGGGCGGCAGCTAGCGGGAGCCGGGACGCCGGTGCAGCCG
CAGCGCGCGGAGGAACCCGGGTGTGCCGGGAGCTGGGCGGCCACGTCCGGACGGGACCGAGACCCCTCGTAGCGCATTGCGGCGACCTCGCCTTCCCCGG
CCGCGAGCGCGCCGCTGCTTGAAAAGCCGCGGAACCCAAGGACTTTTCTCCGGTCCGAGCTCGGGGCGCCCCGCAGGGCGCACGGTACCCGTGCTGCAGT
CGGGCACGCCGCGGCGCCGGGGCCTCCGCAGGGCGATGGAGCCCGGTCTGCAAGGAAAGTGAGGCGCCGCCGCTGCGTTCTGGAGGAGGGGGGCACAAGG
TCTGGAGACCCCGGGTGGCGGACGGGAGCCCTCCCCCCGCCCCGCCTCCGGGGCACCAGCTCCGGCTCCATTGTTCCCGCCCGGGCTGGAGGCGCCGAGC
ACCGAGCGCCGCCGGGAGTCGAGCGCCGGCCGCGGAGCTCTTGCGACCCCGCCAGGACCCGAACAGAGCCCGGGGGCGGCGGGCCGGAGCCGGGGACGCG
GGCACACGCCCGCTCGCACAAGCCACGGCGGACTCTCCCGAGGCGGAACCTCCACGCCGAGCGAGGGTCAGTTTGAAAAGGAGGATCGAGCTCACTGTGG
AGTATCCATGGAGATGTGGAGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGAGCTGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGC
CACACTCTGCACCGCTAGGCCGTCCCCGACCTTGCCTGAACAAGATGCTCTCCCCTCCTCGGAGGATGATGATGATGATGATGACTCCTCTTCAGAGGAG
AAAGAAACAGATAACACCAAACCAAACCCCGTAGCTCCATATTGGACATCCCCAGAAAAGATGGAAAAGAAATTGCATGCAGTGCCGGCTGCCAAGACAG
TGAAGTTCAAATGCCCTTCCAGTGGGACCCCAAACCCCACACTGCGCTGGTTGAAAAATGGCAAAGAATTCAAACCTGACCACAGAATTGGAGGCTACAA
GGTCCGTTATGCCACCTGGAGCATCATAATGGACTCTGTGGTGCCCTCTGACAAGGGCAACTACACCTGCATTGTGGAGAATGAGTACGGCAGCATCAAC
CACACATACCAGCTGGATGTCGTGGAGCGGTCCCCTCACCGGCCCATCCTGCAAGCAGGGTTGCCCGCCAACAAAACAGTGGCCCTGGGTAGCAACGTGG
AGTTCATGTGTAAGGTGTACAGTGACCCGCAGCCGCACATCCAGTGGCTAAAGCACATCGAGGTGAATGGGAGCAAGATTGGCCCAGACAACCTGCCTTA
TGTCCAGATCTTGAAGGTAATCATGGCACCAGTCTTCGTGGGCCAGTCTACTGGGAAGGAGACCACTGTCTCGGGGGCTCAAGTTCCTGTGGGCAGGCTC
AGTTGCCCCCGAATGGGATCATTCCTCACGCTTCAGGCACACACACTCCATCTCAGTAGGGATCTAGCCACATCCCCCAGGACTAGTAACAGAGGTCACA
AAGTGGAGGTGAGCTGGGAACAGAGGGCTGCAGGGATGGGTGGTGCTGGTCTGTAATAAGCTTTGAGAGCAACGTCACTGGGGCTTTGGGGTCAGCTACA
CAAGGAAGGCATTTGGACCCCTGCCTTTTCATTGCCCGAAACCAGAGCCTTTCCACCAAGCGTTTCCCAGTCTTAGCCCTGTGTCCTGAGTTACGTACGA
TCTTTCTGGCAAATGGGGTGCATGATAAGAGCATCTCTTACGAAGAGTTGGAAAAACAAATGCCATATATAAATTCTAAGCCATATGAGGACGAGGAGTA
ATGGCATTTTCTTCCTTTTTCCTCTCACTCCCAGACATTCATTGTCCCTGAATGCTCCATTAATCCAGGGAAGGTAATTGCCTAAATCTCCAGTGGATCT
CGCAACAGGAAGGAACCAGAAGCTGGGAAAGTTGTTTACCTCTTTGTCCCAGAGTTAGACCTCATCCTCCCCTAGCTTAGCTGTCTCAGAGATATACTGG
CCCTCCCTTCTCTTCTCTTTGCTGCTGGTGCTAAAACTGCTCTGTAGGTCATTGGCCACTGTCTCCACTCACAACCCCTGCTCCAGTCCTGGAGGGAGTG
GGTTAAACACAAATAGAACATTCCATTTGAAGCAGTGATTCTTTTTTTTTTTTTTTTTTTTTAATCAAATGCTTTGGACTTTTGAAGTCCACTTGTTCTG
TACTTGTAAAAGGGAAAGAAGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGCACTTTAGATCACTTGAGGTCAGGAGTTTGAGACCAGCCCGGCCAA
CATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGTGCATAGTGGTTGGCACCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAAGCTAACT
GCTTGAACCCAGAAGGCAGAGGTTGCAGTGAGCTGAGATCACGCCACTGCACTCCAGCCTGGGTGACAGAGTGAGTGAGACTCTGCGTT
  • FGFR1.F11 (forward primer)
  • FGFR1.R11 (reverse primer)
  • Single exon skipping

Frame 0

PRRRIALGALLRPQARRPRRRAAASGSRDAGAA
AARGGTRVCRELGGHVRTGPRPLVAHCGDLAFPG
RERAAA*KAAEPKDFSPVRARGAPQGARYPCCS
RARRGAGASAGRWSPVCKESEAPPLRSGGGGHK
VWRPRVADGSPPPAPPPGHQLRLHCSRPGWRRRA
PSAAGSRAPAAELLRPRQDPNRARGRRAGAGDA
GTRPLAQATADSPEAEPPRRARVSLKRRIELTV
EYPWRCGALSPTSNCRTGMWSWKCLLFWAVLVTA
TLCTARPSPTLPEQDALPSSEDDDDDDDSSSEE
KETDNTKPNPVAPYWTSPEKMEKKLHAVPAAKT
VKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYK
VRYATWSIIMDSVVPSDKGNYTCIVENEYGSIN
HTYQLDVVERSPHRPILQAGLPANKTVALGSNV
EFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPY
VQILKVIMAPVFVGQSTGKETTVSGAQVPVGRL
SCPRMGSFLTLQAHTLHLSRDLATSPRTSNRGH
KVEVSWEQRAAGMGGAGL**ALRATSLGLWGQLH
KEGIWTPAFSLPETRAFPPSVSQS*PCVLSYVR
SFWQMGCMIRASLTKSWKNKCHI*ILSHMRTRS
NGIFFLFPLTPRHSLSLNAPLIQGR*LPKSPVDL
ATGRNQKLGKLFTSLSQS*TSSSPSLAVSEIYW
PSLLFSLLLVLKLLCRSLATVSTHNPCSSPGGS
GLNTNRTFHLKQ*FFFFFFFFNQMLWTFEVHLFC
TCKRERRPGAVAHACNPSTLDHLRSGV*DQPGQ
HGETPSLLKIQKLAVHSGWHL*SQLLRRLRQAN
CLNPEGRGCSELRSRHCTPAWVTE*VRLCV

Frame 1

PAGA*RSERSCGHRRGVLGGGRQLAGAGTPVQP
QRAEEPGCAGSWAATSGRDRDPS*RIAATSPSP
AASAPLLEKPRNPRTFLRSELGAPRRAHGTRAAV
GHAAAPGPPQGDGARSARKVRRRRCVLEEGGTR
SGDPGWRTGALPPPRLRGTSSGSIVPARAGGAE
HRAPPGVERRPRSSCDPARTRTEPGGGGPEPGTR
AHARSHKPRRTLPRRNLHAERGSV*KGGSSSLW
SIHGDVEPCHQPLTAELGCGAGSASSSGLCWSQ
PHSAPLGRPRPCLNKMLSPPRRMMMMMMTPLQRR
KKQITPNQTP*LHIGHPQKRWKRNCMQCRLPRQ
*SSNALPVGPQTPHCAG*KMAKNSNLTTELEAT
RSVMPPGAS*WTLWCPLTRATTPALWRMSTAAST
THTSWMSWSGPLTGPSCKQGCPPTKQWPWVATW
SSCVRCTVTRSRTSSG*STSR*MGARLAQTTCL
MSRS*R*SWHQSSWASLLGRRPLSRGLKFLWAGS
VAPEWDHSSRFRHTHSISVGI*PHPPGLVTEVT
KWR*AGNRGLQGWVVLVCNKL*EQRHWGFGVSY
TRKAFGPLPFHCPKPEPFHQAFPSLSPVS*VTYD
LSGKWGA**EHLLRRVGKTNAIYKF*AI*GRGV
MAFSSFFLSLPDIHCP*MLH*SREGNCLNLQWI
SQQEGTRSWESCLPLCPRVRPHPPLA*LSQRYTG
PPFSSLCCWC*NCSVGHWPLSPLTTPAPVLEGV
G*TQIEHSI*SSDSFFFFFFLIKCFGLLKSTCS
VLVKGKEGRAQWLTPVIPAL*IT*GQEFETSPAN
MVKPHLY*KYKN*LCIVVGTCSPSYSGG*GKLT
A*TQKAEVAVS*DHATALQPG*QSE*DSA

Frame 2

PPAHSARSALAATGAASSAAGGS*REPGRRCSR
SARRNPGVPGAGRPRPDGTETPRSALRRPRLPR
PRARRCLKSRGTQGLFSGPSSGRPAGRTVPVLQ
SGTPRRRGLRRAMEPGLQGK*GAAAAFWRRGAQG
LETPGGGREPSPRPASGAPAPAPLFPPGLEAPS
TERRRESSAGRGALATPPGPEQSPGAAGRSRGR
GHTPARTSHGGLSRGGTSTPSEGQFEKEDRAHCG
VSMEMWSLVTNL*LQNWDVELEVPPLLGCAGHS
HTLHR*AVPDLA*TRCSPLLGG******LLFRG
ERNR*HQTKPRSSILDIPRKDGKEIACSAGCQDS
EVQMPFQWDPKPHTALVEKWQRIQT*PQNWRLQ
GPLCHLEHHNGLCGAL*QGQLHLHCGE*VRQHQ
PHIPAGCRGAVPSPAHPASRVARQQNSGPG*QRG
VHV*GVQ*PAAAHPVAKAHRGEWEQDWPRQPAL
CPDLEGNHGTSLRGPVYWEGDHCLGGSSSCGQA
QLPPNGIIPHASGTHTPSQ*GSSHIPQD**QRSQ
SGGELGTEGCRDGWCWSVISFESNVTGALGSAT
QGRHLDPCLFIARNQSLSTKRFPVLALCPELRT
IFLANGVHDKSISYEELEKQMPYINSKPYEDEE*
WHFLPFSSHSQTFIVPECSINPGKVIA*ISSGS
RNRKEPEAGKVVYLFVPELDLILP*LSCLRDIL
ALPSLLFAAGAKTAL*VIGHCLHSQPLLQSWREW
VKHK*NIPFEAVILFFFFFF*SNALDF*SPLVL
YL*KGKKAGRSGSRL*SQHFRSLEVRSLRPARP
TW*NPISTKNTKISCA*WLAPVVPATQEAEAS*L
LEPRRQRLQ*AEITPLHSSLGDRVSETLR

Sequence FGFR1.jAug05

CCCCGCCGGCGCATAGCGCTCGGAGCGCTCTTGCGGCCACAGGCGCGGCGTCCTCGGCGGCGGGCGGCAGCTAGCGGGAGCCGGGACGCCGGTGCAGCCG
CAGCGCGCGGAGGAACCCGGGTGTGCCGGGAGCTGGGCGGCCACGTCCGGACGGGACCGAGACCCCTCGTAGCGCATTGCGGCGACCTCGCCTTCCCCGG
CCGCGAGCGCGCCGCTGCTTGAAAAGCCGCGGAACCCAAGGACTTTTCTCCGGTCCGAGCTCGGGGCGCCCCGCAGGGCGCACGGTACCCGTGCTGCAGT
CGGGCACGCCGCGGCGCCGGGGCCTCCGCAGGGCGATGGAGCCCGGTCTGCAAGGAAAGTGAGGCGCCGCCGCTGCGTTCTGGAGGAGGGGGGCACAAGG
TCTGGAGACCCCGGGTGGCGGACGGGAGCCCTCCCCCCGCCCCGCCTCCGGGGCACCAGCTCCGGCTCCATTGTTCCCGCCCGGGCTGGAGGCGCCGAGC
ACCGAGCGCCGCCGGGAGTCGAGCGCCGGCCGCGGAGCTCTTGCGACCCCGCCAGGACCCGAACAGAGCCCGGGGGCGGCGGGCCGGAGCCGGGGACGCG
GGCACACGCCCGCTCGCACAAGCCACGGCGGACTCTCCCGAGGCGGAACCTCCACGCCGAGCGAGGGTCAGTTTGAAAAGGAGGATCGAGCTCACTGTGG
AGTATCCATGGAGATGTGGAGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGAGCTGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGC
CACACTCTGCACCGCTAGGCCGTCCCCGACCTTGCCTGAACAAGATGCTCTCCCCTCCTCGGAGGATGATGATGATGATGATGACTCCTCTTCAGAGGAG
AAAGAAACAGATAACACCAAACCAAACCGTATGCCCGTAGCTCCATATTGGACATCCCCAGAAAAGATGGAAAAGAAATTGCATGCAGTGCCGGCTGCCA
AGACAGTGAAGTTCAAATGCCCTTCCAGTGGGACCCCAAACCCCACACTGCGCTGGTTGAAAAATGGCAAAGAATTCAAACCTGACCACAGAATTGGAGG
CTACAAGGTCCGTTATGCCACCTGGAGCATCATAATGGACTCTGTGGTGCCCTCTGACAAGGGCAACTACACCTGCATTGTGGAGAATGAGTACGGCAGC
ATCAACCACACATACCAGCTGGATGTCGTGGAGCGGTCCCCTCACCGGCCCATCCTGCAAGCAGGGTTGCCCGCCAACAAAACAGTGGCCCTGGGTAGCA
ACGTGGAGTTCATGTGTAAGGTGTACAGTGACCCGCAGCCGCACATCCAGTGGCTAAAGCACATCGAGGTGAATGGGAGCAAGATTGGCCCAGACAACCT
GCCTTATGTCCAGATCTTGAAGGTAATCATGGCACCAGTCTTCGTGGGCCAGTCTACTGGGAAGGAGACCACTGTCTCGGGGGCTCAAGTTCCTGTGGGC
AGGCTCAGTTGCCCCCGAATGGGATCATTCCTCACGCTTCAGGCACACACACTCCATCTCAGTAGGGATCTAGCCACATCCCCCAGGACTAGTAACAGAG
GTCACAAAGTGGAGGTGAGCTGGGAACAGAGGGCTGCAGGGATGGGTGGTGCTGGTCTGTAATAAGCTTTGAGAGCAACGTCACTGGGGCTTTGGGGTCA
GCTACACAAGGAAGGCATTTGGACCCCTGCCTTTTCATTGCCCGAAACCAGAGCCTTTCCACCAAGCGTTTCCCAGTCTTAGCCCTGTGTCCTGAGTTAC
GTACGATCTTTCTGGCAAATGGGGTGCATGATAAGAGCATCTCTTACGAAGAGTTGGAAAAACAAATGCCATATATAAATTCTAAGCCATATGAGGACGA
GGAGTAATGGCATTTTCTTCCTTTTTCCTCTCACTCCCAGACATTCATTGTCCCTGAATGCTCCATTAATCCAGGGAAGGTAATTGCCTAAATCTCCAGT
GGATCTCGCAACAGGAAGGAACCAGAAGCTGGGAAAGTTGTTTACCTCTTTGTCCCAGAGTTAGACCTCATCCTCCCCTAGCTTAGCTGTCTCAGAGATA
TACTGGCCCTCCCTTCTCTTCTCTTTGCTGCTGGTGCTAAAACTGCTCTGTAGGTCATTGGCCACTGTCTCCACTCACAACCCCTGCTCCAGTCCTGGAG
GGAGTGGGTTAAACACAAATAGAACATTCCATTTGAAGCAGTGATTCTTTTTTTTTTTTTTTTTTTTTAATCAAATGCTTTGGACTTTTGAAGTCCACTT
GTTCTGTACTTGTAAAAGGGAAAGAAGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGCACTTTAGATCACTTGAGGTCAGGAGTTTGAGACCAGCCC
GGCCAACATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGTGCATAGTGGTTGGCACCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAAG
CTAACTGCTTGAACCCAGAAGGCAGAGGTTGCAGTGAGCTGAGATCACGCCACTGCACTCCAGCCTGGGTGACAGAGTGAGTGAGACTCTGCGTT
  • FGFR1.F11 (forward primer)
  • FGFR1.R11 (reverse primer)
  • Single exon skipping

Frame 0

PRRRIALGALLRPQARRPRRRAAASGSRDAGAA
AARGGTRVCRELGGHVRTGPRPLVAHCGDLAFPG
RERAAA*KAAEPKDFSPVRARGAPQGARYPCCS
RARRGAGASAGRWSPVCKESEAPPLRSGGGGHK
VWRPRVADGSPPPAPPPGHQLRLHCSRPGWRRRA
PSAAGSRAPAAELLRPRQDPNRARGRRAGAGDA
GTRPLAQATADSPEAEPPRRARVSLKRRIELTV
EYPWRCGALSPTSNCRTGMWSWKCLLFWAVLVTA
TLCTARPSPTLPEQDALPSSEDDDDDDDSSSEE
KETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAA
KTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGG
YKVRYATWSIIMDSVVPSDKGNYTCIVENEYGS
INHTYQLDVVERSPHRPILQAGLPANKTVALGS
NVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNL
PYVQILKVIMAPVFVGQSTGKETTVSGAQVPVG
RLSCPRMGSFLTLQAHTLHLSRDLATSPRTSNR
GHKVEVSWEQRAAGMGGAGL**ALRATSLGLWGQ
LHKEGIWTPAFSLPETRAFPPSVSQS*PCVLSY
VRSFWQMGCMIRASLTKSWKNKCHI*ILSHMRT
RSNGIFFLFPLTPRHSLSLNAPLIQGR*LPKSPV
DLATGRNQKLGKLFTSLSQS*TSSSPSLAVSEI
YWPSLLFSLLLVLKLLCRSLATVSTHNPCSSPG
GSGLNTNRTFHLKQ*FFFFFFFFNQMLWTFEVHL
FCTCKRERRPGAVAHACNPSTLDHLRSGV*DQP
GQHGETPSLLKIQKLAVHSGWHL*SQLLRRLRQ
ANCLNPEGRGCSELRSRHCTPAWVTE*VRLCV

Frame 1

PAGA*RSERSCGHRRGVLGGGRQLAGAGTPVQP
QRAEEPGCAGSWAATSGRDRDPS*RIAATSPSP
AASAPLLEKPRNPRTFLRSELGAPRRAHGTRAAV
GHAAAPGPPQGDGARSARKVRRRRCVLEEGGTR
SGDPGWRTGALPPPRLRGTSSGSIVPARAGGAE
HRAPPGVERRPRSSCDPARTRTEPGGGGPEPGTR
AHARSHKPRRTLPRRNLHAERGSV*KGGSSSLW
SIHGDVEPCHQPLTAELGCGAGSASSSGLCWSQ
PHSAPLGRPRPCLNKMLSPPRRMMMMMMTPLQRR
KKQITPNQTVCP*LHIGHPQKRWKRNCMQCRLP
RQ*SSNALPVGPQTPHCAG*KMAKNSNLTTELE
ATRSVMPPGAS*WTLWCPLTRATTPALWRMSTAA
STTHTSWMSWSGPLTGPSCKQGCPPTKQWPWVA
TWSSCVRCTVTRSRTSSG*STSR*MGARLAQTT
CLMSRS*R*SWHQSSWASLLGRRPLSRGLKFLWA
GSVAPEWDHSSRFRHTHSISVGI*PHPPGLVTE
VTKWR*AGNRGLQGWVVLVCNKL*EQRHWGFGV
SYTRKAFGPLPFHCPKPEPFHQAFPSLSPVS*VT
YDLSGKWGA**EHLLRRVGKTNAIYKF*AI*GR
GVMAFSSFFLSLPDIHCP*MLH*SREGNCLNLQ
WISQQEGTRSWESCLPLCPRVRPHPPLA*LSQRY
TGPPFSSLCCWC*NCSVGHWPLSPLTTPAPVLE
GVG*TQIEHSI*SSDSFFFFFFLIKCFGLLKST
CSVLVKGKEGRAQWLTPVIPAL*IT*GQEFETSP
ANMVKPHLY*KYKN*LCIVVGTCSPSYSGG*GK
LTA*TQKAEVAVS*DHATALQPG*QSE*DSA

Frame 2

PPAHSARSALAATGAASSAAGGS*REPGRRCSR
SARRNPGVPGAGRPRPDGTETPRSALRRPRLPR
PRARRCLKSRGTQGLFSGPSSGRPAGRTVPVLQ
SGTPRRRGLRRAMEPGLQGK*GAAAAFWRRGAQG
LETPGGGREPSPRPASGAPAPAPLFPPGLEAPS
TERRRESSAGRGALATPPGPEQSPGAAGRSRGR
GHTPARTSHGGLSRGGTSTPSEGQFEKEDRAHCG
VSMEMWSLVTNL*LQNWDVELEVPPLLGCAGHS
HTLHR*AVPDLA*TRCSPLLGG******LLFRG
ERNR*HQTKPYARSSILDIPRKDGKEIACSAGCQ
DSEVQMPFQWDPKPHTALVEKWQRIQT*PQNWR
LQGPLCHLEHHNGLCGAL*QGQLHLHCGE*VRQ
HQPHIPAGCRGAVPSPAHPASRVARQQNSGPG*Q
RGVHV*GVQ*PAAAHPVAKAHRGEWEQDWPRQP
ALCPDLEGNHGTSLRGPVYWEGDHCLGGSSSCG
QAQLPPNGIIPHASGTHTPSQ*GSSHIPQD**QR
SQSGGELGTEGCRDGWCWSVISFESNVTGALGS
ATQGRHLDPCLFIARNQSLSTKRFPVLALCPEL
RTIFLANGVHDKSISYEELEKQMPYINSKPYEDE
E*WHFLPFSSHSQTFIVPECSINPGKVIA*ISS
GSRNRKEPEAGKVVYLFVPELDLILP*LSCLRD
ILALPSLLFAAGAKTAL*VIGHCLHSQPLLQSWR
EWVKHK*NIPFEAVILFFFFFF*SNALDF*SPL
VLYL*KGKKAGRSGSRL*SQHFRSLEVRSLRPA
RPTW*NPISTKNTKISCA*WLAPVVPATQEAEAS
*LLEPRRQRLQ*AEITPLHSSLGDRVSETLR

Sequence FGFR1.eAug05

CCCCGCCGGCGCATAGCGCTCGGAGCGCTCTTGCGGCCACAGGCGCGGCGTCCTCGGCGGCGGGCGGCAGCTAGCGGGAGCCGGGACGCCGGTGCAGCCG
CAGCGCGCGGAGGAACCCGGGTGTGCCGGGAGCTGGGCGGCCACGTCCGGACGGGACCGAGACCCCTCGTAGCGCATTGCGGCGACCTCGCCTTCCCCGG
CCGCGAGCGCGCCGCTGCTTGAAAAGCCGCGGAACCCAAGGACTTTTCTCCGGTCCGAGCTCGGGGCGCCCCGCAGGGCGCACGGTACCCGTGCTGCAGT
CGGGCACGCCGCGGCGCCGGGGCCTCCGCAGGGCGATGGAGCCCGGTCTGCAAGGAAAGTGAGGCGCCGCCGCTGCGTTCTGGAGGAGGGGGGCACAAGG
TCTGGAGACCCCGGGTGGCGGACGGGAGCCCTCCCCCCGCCCCGCCTCCGGGGCACCAGCTCCGGCTCCATTGTTCCCGCCCGGGCTGGAGGCGCCGAGC
ACCGAGCGCCGCCGGGAGTCGAGCGCCGGCCGCGGAGCTCTTGCGACCCCGCCAGGACCCGAACAGAGCCCGGGGGCGGCGGGCCGGAGCCGGGGACGCG
GGCACACGCCCGCTCGCACAAGCCACGGCGGACTCTCCCGAGGCGGAACCTCCACGCCGAGCGAGGGTCAGTTTGAAAAGGAGGATCGAGCTCACTGTGG
AGTATCCATGGAGATGTGGAGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGAGCTGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGC
CACACTCTGCACCGCTAGGCCGTCCCCGACCTTGCCTGAACAAGATGCTCTCCCCTCCTCGGAGGATGATGATGATGATGATGACTCCTCTTCAGAGGAG
AAAGAAACAGATAACACCAAACCAAACCCCGTAGCTCCATATTGGACATCCCCAGAAAAGATGGAAAAGAAATTGCATGCAGTGCCGGCTGCCAAGACAG
TGAAGTTCAAATGCCCTTCCAGTGGGACCCCAAACCCCACACTGCGCTGGTTGAAAAATGGCAAAGAATTCAAACCTGACCACAGAATTGGAGGCTACAA
GGTCCGTTATGCCACCTGGAGCATCATAATGGACTCTGTGGTGCCCTCTGACAAGGGCAACTACACCTGCATTGTGGAGAATGAGTACGGCAGCATCAAC
CACACATACCAGCTGGATGTCGTGGAGCGGTCCCCTCACCGGCCCATCCTGCAAGCAGGGTTGCCCGCCAACAAAACAGTGGCCCTGGGTAGCAACGTGG
AGTTCATGTGTAAGGTGTACAGTGACCCGCAGCCGCACATCCAGTGGCTAAAGCACATCGAGGTGAATGGGAGCAAGATTGGCCCAGACAACCTGCCTTA
TGTCCAGATCTTGAAGACTGCTGGAGTTAATACCACCGACAAAGAGATGGAGGTGCTTCACTTAAGAAATGTCTCCTTTGAGGACGCAGGGGAGTATACG
TGCTTGGCGGGTAACTCTATCGGACTCTCCCATCACTCTGCATGGTTGACCGTTCTGGAAGCCCTGGAAGAGAGGCCGGCAGTGATGACCTCGCCCCTGT
ACCTGGAGATCATCATCTATTGCACAGGGGCCTTCCTCATCTCCTGCATGGTGGGGTCGGTCATCGTCTACAAGATGAAGAGTGGTACCAAGAAGAGTGA
CTTCCACAGCCAGATGGCTGTGCACAAGCTGGCCAAGAGCATCCCTCTGCGCAGACAGGTAACAGTGTCTGCTGACTCCAGTGCATCCATGAACTCTGGG
GTTCTTCTGGTTCGGCCATCACGGCTCTCCTCCAGTGGGACTCCCATGCTAGCAGGGGTCTCTGAGTATGAGCTTCCCGAAGACCCTCGCTGGGAGCTGC
CTCGGGACAGACTGGTCTTAGGCAAACCCCTGGGAGAGGGCTGCTTTGGGCAGGTGGTGTTGGCAGAGGCTATCGGGCTGGACAAGGACAAACCCAACCG
TGTGACCAAAGTGGCTGTGAAGATGTTGAAGTCGGACGCAACAGAGAAAGACTTGTCAGACCTGATCTCAGAAATGGAGATGATGAAGATGATCGGGAAG
CATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTTGTATGTCATCGTGGAGTATGCCTCCAAGGGCAACCTGCGGGAGTACCTGC
AGGCCCGGAGGCCCCCAGGGCTGGAATACTGCTACAACCCCAGCCACAACCCAGAGGAGCAGCTCTCCTCCAAGGACCTGGTGTCCTGCGCCTACCAGGT
GGCCCGAGGCATGGAGTATCTGGCCTCCAAGAAGTGCATACACCGAGACCTGGCAGCCAGGAATGTCCTGGTGACAGAGGACAATGTGATGAAGATAGCA
GACTTTGGCCTCGCACGGGACATTCACCACATCGACTACTATAAAAAGACAACCAACGGCCGACTGCCTGTGAAGTGGATGGCACCCGAGGCATTATTTG
ACCGGATCTACACCCACCAGAGTGATGTGTGGTCTTTCGGGGTGCTCCTGTGGGAGATCTTCACTCTGGGCGGCTCCCCATACCCCGGTGTGCCTGTGGA
GGAACTTTTCAAGCTGCTGAAGGAGGGTCACCGCATGGACAAGCCCAGTAACTGCACCAACGAGCTGTACATGATGATGCGGGACTGCTGGCATGCAGTG
CCCTCACAGAGACCCACCTTCAAGCAGCTGGTGGAAGACCTGGACCGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCCCCTGG
ACCAGTACTCCCCCAGCTTTCCCGACACCCGGAGCTCTACGTGCTCCTCAGGGGAGGATTCCGTCTTCTCTCATGAGCCGCTGCCCGAGGAGCCCTGCCT
GCCCCGACACCCAGCCCAGCTTGCCAATGGCGGACTCAAACGCCGCTGACTGCCACCCACACGCCCTCCCCAGACTCCACCGTCAGCTGTAACCCTCACC
CACAGCCCCTGCTGGGCCCACCACCTGTCCGTCCCTGTCCCCTTTCCTGCTGGCAGGAGCCGGCTGCCTACCAGGGGCCTTCCTGTGTGGCCTGCCTTCA
CCCCACTCAGCTCACCTCTCCCTCCACCTCCTCTCCACCTGCTGGTGAGAGGTGCAAAGAGGCAGATCTTTGCTGCCAGCCACTTCATCCCCTCCCAGAT
GTTGGACCAACACCCCTCCCTGCCACCAGGCACTGCCTGGAGGGCAGGGAGTGGGAGCCAATGAACAGGCATGCAAGTGAGAGCTTCCTGAGCTTTCTCC
TGTCGGTTTGGTCTGTTTTGCCTTCACCCATAAGCCCCTCGCACTCTGGTGGCAGGTGCCTTGTCCTCAGGGCTACAGCAGTAGGGAGGTCAGTGCTTCG
TGCCTCGATTGAAGGTGACCTCTGCCCCAGATAGGTGGTGCCAGTGGCTTATTAATTCCGATACTAGTTTGCTTTGCTGACCAAATGCCTGGTACCAGAG
GATGGTGAGGCGAAGGCCAGGTTGGGGGCAGTGTTGTGGCCCTGGGGCCCAGCCCCAAACTGGGGGCTCTGTATATAGCTATGAAGAAAACACAAAGTGT
ATAAATCTGAGTATATATTTACATGTCTTTTTAAAAGGGTCGTTACCAGAGATTTACCCATCGGGTAAGATGCTCCTGGTGGCTGGGAGGCATCAGTTGC
TATATATTAAAAACAAAAAAGAAAAAAAAGGAAAATGTTTTTAAAAAGGTCATATATTTTTTGCTACTTTTGCTGTTTTATTTTTTTAAATTATGTTCTA
AACCTATTTTCAGTTTAGGTCCCTCAATAAAAATTGCTGCTGCTTC
  • FGFR1.F11 (forward primer)
  • FGFR1.R11 (reverse primer)
  • Single exon skipping

Frame 0

PRRRIALGALLRPQARRPRRRAAASGSRDAGAA
AARGGTRVCRELGGHVRTGPRPLVAHCGDLAFPG
RERAAA*KAAEPKDFSPVRARGAPQGARYPCCS
RARRGAGASAGRWSPVCKESEAPPLRSGGGGHK
VWRPRVADGSPPPAPPPGHQLRLHCSRPGWRRRA
PSAAGSRAPAAELLRPRQDPNRARGRRAGAGDA
GTRPLAQATADSPEAEPPRRARVSLKRRIELTV
EYPWRCGALSPTSNCRTGMWSWKCLLFWAVLVTA
TLCTARPSPTLPEQDALPSSEDDDDDDDSSSEE
KETDNTKPNPVAPYWTSPEKMEKKLHAVPAAKT
VKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYK
VRYATWSIIMDSVVPSDKGNYTCIVENEYGSIN
HTYQLDVVERSPHRPILQAGLPANKTVALGSNV
EFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPY
VQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYT
CLAGNSIGLSHHSAWLTVLEALEERPAVMTSPL
YLEIIIYCTGAFLISCMVGSVIVYKMKSGTKKSD
FHSQMAVHKLAKSIPLRRQVTVSADSSASMNSG
VLLVRPSRLSSSGTPMLAGVSEYELPEDPRWEL
PRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNR
VTKVAVKMLKSDATEKDLSDLISEMEMMKMIGK
HKNIINLLGACTQDGPLYVIVEYASKGNLREYL
QARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQV
ARGMEYLASKKCIHRDLAARNVLVTEDNVMKIA
DFGLARDIHHIDYYKKTTNGRLPVKWMAPEALF
DRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVE
ELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAV
PSQRPTFKQLVEDLDRIVALTSNQEYLDLSMPL
DQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCL
PRHPAQLANGGLKRR*LPPTRPPQTPPSAVTLT
HSPCWAHHLSVPVPFPAGRSRLPTRGLPVWPAF
TPLSSPLPPPPLHLLVRGAKRQIFAASHFIPSQM
LDQHPSLPPGTAWRAGSGSQ*TGMQVRAS*AFS
CRFGLFCLHP*APRTLVAGALSSGLQQ*GGQCF
VPRLKVTSAPDRWCQWLINSDTSLLC*PNAWYQR
MVRRRPGWGQCCGPGAQPQTGGSVYSYEENTKC
INLSIYLHVFLKGSLPEIYPSGKMLLVAGRHQL
LYIKNKKEKKGKCF*KGHIFFATFAVLFF*IMF*
TYFQFRSLNKNCCCF

Frame 1

PAGA*RSERSCGHRRGVLGGGRQLAGAGTPVQP
QRAEEPGCAGSWAATSGRDRDPS*RIAATSPSP
AASAPLLEKPRNPRTFLRSELGAPRRAHGTRAAV
GHAAAPGPPQGDGARSARKVRRRRCVLEEGGTR
SGDPGWRTGALPPPRLRGTSSGSIVPARAGGAE
HRAPPGVERRPRSSCDPARTRTEPGGGGPEPGTR
AHARSHKPRRTLPRRNLHAERGSV*KGGSSSLW
SIHGDVEPCHQPLTAELGCGAGSASSSGLCWSQ
PHSAPLGRPRPCLNKMLSPPRRMMMMMMTPLQRR
KKQITPNQTP*LHIGHPQKRWKRNCMQCRLPRQ
*SSNALPVGPQTPHCAG*KMAKNSNLTTELEAT
RSVMPPGAS*WTLWCPLTRATTPALWRMSTAAST
THTSWMSWSGPLTGPSCKQGCPPTKQWPWVATW
SSCVRCTVTRSRTSSG*STSR*MGARLAQTTCL
MSRS*RLLELIPPTKRWRCFT*EMSPLRTQGSIR
AWRVTLSDSPITLHG*PFWKPWKRGRQ**PRPC
TWRSSSIAQGPSSSPAWWGRSSSTR*RVVPRRV
TSTARWLCTSWPRASLCADR*QCLLTPVHP*TLG
FFWFGHHGSPPVGLPC*QGSLSMSFPKTLAGSC
LGTDWS*ANPWERAALGRWCWQRLSGWTRTNPT
V*PKWL*RC*SRTQQRKTCQT*SQKWR**R*SGS
IRISSTCWGPARRMVPCMSSWSMPPRATCGSTC
RPGGPQGWNTATTPATTQRSSSPPRTWCPAPTR
WPEAWSIWPPRSAYTETWQPGMSW*QRTM**R*Q
TLASHGTFTTSTTIKRQPTADCL*SGWHPRHYL
TGSTPTRVMCGLSGCSCGRSSLWAAPHTPVCLW
RNFSSC*RRVTAWTSPVTAPTSCT**CGTAGMQC
PHRDPPSSSWWKTWTASWP*PPTRSTWTCPCPW
TSTPPAFPTPGALRAPQGRIPSSLMSRCPRSPA
CPDTQPSLPMADSNAADCHPHALPRLHRQL*PSP
TAPAGPTTCPSLSPFLLAGAGCLPGAFLCGLPS
PHSAHLSLHLLSTCW*EVQRGRSLLPATSSPPR
CWTNTPPCHQALPGGQGVGANEQACK*ELPELSP
VGLVCFAFTHKPLALWWQVPCPQGYSSREVSAS
CLD*R*PLPQIGGASGLLIPILVCFADQMPGTR
GW*GEGQVGGSVVALGPSPKLGALYIAMKKTQSV
*I*VYIYMSF*KGRYQRFTHRVRCSWWLGGISC
YILKTKKKKKENVFKKVIYFLLLLLFYFFKLCS
KPIFSLGPSIKIAAA

Frame 2

PPAHSARSALAATGAASSAAGGS*REPGRRCSR
SARRNPGVPGAGRPRPDGTETPRSALRRPRLPR
PRARRCLKSRGTQGLFSGPSSGRPAGRTVPVLQ
SGTPRRRGLRRAMEPGLQGK*GAAAAFWRRGAQG
LETPGGGREPSPRPASGAPAPAPLFPPGLEAPS
TERRRESSAGRGALATPPGPEQSPGAAGRSRGR
GHTPARTSHGGLSRGGTSTPSEGQFEKEDRAHCG
VSMEMWSLVTNL*LQNWDVELEVPPLLGCAGHS
HTLHR*AVPDLA*TRCSPLLGG******LLFRG
ERNR*HQTKPRSSILDIPRKDGKEIACSAGCQDS
EVQMPFQWDPKPHTALVEKWQRIQT*PQNWRLQ
GPLCHLEHHNGLCGAL*QGQLHLHCGE*VRQHQ
PHIPAGCRGAVPSPAHPASRVARQQNSGPG*QRG
VHV*GVQ*PAAAHPVAKAHRGEWEQDWPRQPAL
CPDLEDCWS*YHRQRDGGASLKKCLL*GRRGVY
VLGG*LYRTLPSLCMVDRSGSPGREAGSDDLAPV
PGDHHLLHRGLPHLLHGGVGHRLQDEEWYQEE*
LPQPDGCAQAGQEHPSAQTGNSVC*LQCIHELW
GSSGSAITALLQWDSHASRGL*V*ASRRPSLGAA
SGQTGLRQTPGRGLLWAGGVGRGYRAGQGQTQP
CDQSGCEDVEVGRNRERLVRPDLRNGDDEDDRE
A*EYHQPAGGLHAGWSLVCHRGVCLQGQPAGVPA
GPEAPRAGILLQPQPQPRGAALLQGPGVLRLPG
GPRHGVSGLQEVHTPRPGSQECPGDRGQCDEDS
RLWPRTGHSPHRLL*KDNQRPTACEVDGTRGII*
PDLHPPE*CVVFRGAPVGDLHSGRLPIPRCACG
GTFQAAEGGSPHGQAQ*LHQRAVHDDAGLLACS
ALTETHLQAAGGRPGPHRGLDLQPGVPGPVHAPG
PVLPQLSRHPELYVLLRGGFRLLS*AAARGALP
APTPSPACQWRTQTPLTATHTPSPDSTVSCNPH
PQPLLGPPPVRPCPLSCWQEPAAYQGPSCVACLH
PTQLTSPSTSSPPAGERCKEADLCCQPLHPLPD
VGPTPLPATRHCLEGREWEPMNRHASESFLSFL
LSVWSVLPSPISPSHSGGRCLVLRATAVGRSVLR
ASIEGDLCPR*VVPVAY*FRY*FALLTKCLVPE
DGEAKARLGAVLWPWGPAPNWGLCI*L*RKHKV
YKSEYIFTCLFKRVVTRDLPIG*DAPGGWEASVA
IY*KQKRKKRKMFLKRSYIFCYFCCFIFLNYVL
NLFSV*VPQ*KLLLL

Sequence FGFR1.dAug05

CCCCGCCGGCGCATAGCGCTCGGAGCGCTCTTGCGGCCACAGGCGCGGCGTCCTCGGCGGCGGGCGGCAGCTAGCGGGAGCCGGGACGCCGGTGCAGCCG
CAGCGCGCGGAGGAACCCGGGTGTGCCGGGAGCTGGGCGGCCACGTCCGGACGGGACCGAGACCCCTCGTAGCGCATTGCGGCGACCTCGCCTTCCCCGG
CCGCGAGCGCGCCGCTGCTTGAAAAGCCGCGGAACCCAAGGACTTTTCTCCGGTCCGAGCTCGGGGCGCCCCGCAGGGCGCACGGTACCCGTGCTGCAGT
CGGGCACGCCGCGGCGCCGGGGCCTCCGCAGGGCGATGGAGCCCGGTCTGCAAGGAAAGTGAGGCGCCGCCGCTGCGTTCTGGAGGAGGGGGGCACAAGG
TCTGGAGACCCCGGGTGGCGGACGGGAGCCCTCCCCCCGCCCCGCCTCCGGGGCACCAGCTCCGGCTCCATTGTTCCCGCCCGGGCTGGAGGCGCCGAGC
ACCGAGCGCCGCCGGGAGTCGAGCGCCGGCCGCGGAGCTCTTGCGACCCCGCCAGGACCCGAACAGAGCCCGGGGGCGGCGGGCCGGAGCCGGGGACGCG
GGCACACGCCCGCTCGCACAAGCCACGGCGGACTCTCCCGAGGCGGAACCTCCACGCCGAGCGAGGGTCAGTTTGAAAAGGAGGATCGAGCTCACTGTGG
AGTATCCATGGAGATGTGGAGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGAGCTGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGC
CACACTCTGCACCGCTAGGCCGTCCCCGACCTTGCCTGAACAAGATGCTCTCCCCTCCTCGGAGGATGATGATGATGATGATGACTCCTCTTCAGAGGAG
AAAGAAACAGATAACACCAAACCAAACCGTATGCCCGTAGCTCCATATTGGACATCCCCAGAAAAGATGGAAAAGAAATTGCATGCAGTGCCGGCTGCCA
AGACAGTGAAGTTCAAATGCCCTTCCAGTGGGACCCCAAACCCCACACTGCGCTGGTTGAAAAATGGCAAAGAATTCAAACCTGACCACAGAATTGGAGG
CTACAAGGTCCGTTATGCCACCTGGAGCATCATAATGGACTCTGTGGTGCCCTCTGACAAGGGCAACTACACCTGCATTGTGGAGAATGAGTACGGCAGC
ATCAACCACACATACCAGCTGGATGTCGTGGAGCGGTCCCCTCACCGGCCCATCCTGCAAGCAGGGTTGCCCGCCAACAAAACAGTGGCCCTGGGTAGCA
ACGTGGAGTTCATGTGTAAGGTGTACAGTGACCCGCAGCCGCACATCCAGTGGCTAAAGCACATCGAGGTGAATGGGAGCAAGATTGGCCCAGACAACCT
GCCTTATGTCCAGATCTTGAAGACTGCTGGAGTTAATACCACCGACAAAGAGATGGAGGTGCTTCACTTAAGAAATGTCTCCTTTGAGGACGCAGGGGAG
TATACGTGCTTGGCGGGTAACTCTATCGGACTCTCCCATCACTCTGCATGGTTGACCGTTCTGGAAGCCCTGGAAGAGAGGCCGGCAGTGATGACCTCGC
CCCTGTACCTGGAGATCATCATCTATTGCACAGGGGCCTTCCTCATCTCCTGCATGGTGGGGTCGGTCATCGTCTACAAGATGAAGAGTGGTACCAAGAA
GAGTGACTTCCACAGCCAGATGGCTGTGCACAAGCTGGCCAAGAGCATCCCTCTGCGCAGACAGGTAACAGTGTCTGCTGACTCCAGTGCATCCATGAAC
TCTGGGGTTCTTCTGGTTCGGCCATCACGGCTCTCCTCCAGTGGGACTCCCATGCTAGCAGGGGTCTCTGAGTATGAGCTTCCCGAAGACCCTCGCTGGG
AGCTGCCTCGGGACAGACTGGTCTTAGGCAAACCCCTGGGAGAGGGCTGCTTTGGGCAGGTGGTGTTGGCAGAGGCTATCGGGCTGGACAAGGACAAACC
CAACCGTGTGACCAAAGTGGCTGTGAAGATGTTGAAGTCGGACGCAACAGAGAAAGACTTGTCAGACCTGATCTCAGAAATGGAGATGATGAAGATGATC
GGGAAGCATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTTGTATGTCATCGTGGAGTATGCCTCCAAGGGCAACCTGCGGGAGT
ACCTGCAGGCCCGGAGGCCCCCAGGGCTGGAATACTGCTACAACCCCAGCCACAACCCAGAGGAGCAGCTCTCCTCCAAGGACCTGGTGTCCTGCGCCTA
CCAGGTGGCCCGAGGCATGGAGTATCTGGCCTCCAAGAAGTGCATACACCGAGACCTGGCAGCCAGGAATGTCCTGGTGACAGAGGACAATGTGATGAAG
ATAGCAGACTTTGGCCTCGCACGGGACATTCACCACATCGACTACTATAAAAAGACAACCAACGGCCGACTGCCTGTGAAGTGGATGGCACCCGAGGCAT
TATTTGACCGGATCTACACCCACCAGAGTGATGTGTGGTCTTTCGGGGTGCTCCTGTGGGAGATCTTCACTCTGGGCGGCTCCCCATACCCCGGTGTGCC
TGTGGAGGAACTTTTCAAGCTGCTGAAGGAGGGTCACCGCATGGACAAGCCCAGTAACTGCACCAACGAGCTGTACATGATGATGCGGGACTGCTGGCAT
GCAGTGCCCTCACAGAGACCCACCTTCAAGCAGCTGGTGGAAGACCTGGACCGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGC
CCCTGGACCAGTACTCCCCCAGCTTTCCCGACACCCGGAGCTCTACGTGCTCCTCAGGGGAGGATTCCGTCTTCTCTCATGAGCCGCTGCCCGAGGAGCC
CTGCCTGCCCCGACACCCAGCCCAGCTTGCCAATGGCGGACTCAAACGCCGCTGACTGCCACCCACACGCCCTCCCCAGACTCCACCGTCAGCTGTAACC
CTCACCCACAGCCCCTGCTGGGCCCACCACCTGTCCGTCCCTGTCCCCTTTCCTGCTGGCAGGAGCCGGCTGCCTACCAGGGGCCTTCCTGTGTGGCCTG
CCTTCACCCCACTCAGCTCACCTCTCCCTCCACCTCCTCTCCACCTGCTGGTGAGAGGTGCAAAGAGGCAGATCTTTGCTGCCAGCCACTTCATCCCCTC
CCAGATGTTGGACCAACACCCCTCCCTGCCACCAGGCACTGCCTGGAGGGCAGGGAGTGGGAGCCAATGAACAGGCATGCAAGTGAGAGCTTCCTGAGCT
TTCTCCTGTCGGTTTGGTCTGTTTTGCCTTCACCCATAAGCCCCTCGCACTCTGGTGGCAGGTGCCTTGTCCTCAGGGCTACAGCAGTAGGGAGGTCAGT
GCTTCGTGCCTCGATTGAAGGTGACCTCTGCCCCAGATAGGTGGTGCCAGTGGCTTATTAATTCCGATACTAGTTTGCTTTGCTGACCAAATGCCTGGTA
CCAGAGGATGGTGAGGCGAAGGCCAGGTTGGGGGCAGTGTTGTGGCCCTGGGGCCCAGCCCCAAACTGGGGGCTCTGTATATAGCTATGAAGAAAACACA
AAGTGTATAAATCTGAGTATATATTTACATGTCTTTTTAAAAGGGTCGTTACCAGAGATTTACCCATCGGGTAAGATGCTCCTGGTGGCTGGGAGGCATC
AGTTGCTATATATTAAAAACAAAAAAGAAAAAAAAGGAAAATGTTTTTAAAAAGGTCATATATTTTTTGCTACTTTTGCTGTTTTATTTTTTTAAATTAT
GTTCTAAACCTATTTTCAGTTTAGGTCCCTCAATAAAAATTGCTGCTGCTTC
  • FGFR1.F11 (forward primer)
  • FGFR1.R11 (reverse primer)
  • Single exon skipping

Frame 0

PRRRIALGALLRPQARRPRRRAAASGSRDAGAA
AARGGTRVCRELGGHVRTGPRPLVAHCGDLAFPG
RERAAA*KAAEPKDFSPVRARGAPQGARYPCCS
RARRGAGASAGRWSPVCKESEAPPLRSGGGGHK
VWRPRVADGSPPPAPPPGHQLRLHCSRPGWRRRA
PSAAGSRAPAAELLRPRQDPNRARGRRAGAGDA
GTRPLAQATADSPEAEPPRRARVSLKRRIELTV
EYPWRCGALSPTSNCRTGMWSWKCLLFWAVLVTA
TLCTARPSPTLPEQDALPSSEDDDDDDDSSSEE
KETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAA
KTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGG
YKVRYATWSIIMDSVVPSDKGNYTCIVENEYGS
INHTYQLDVVERSPHRPILQAGLPANKTVALGS
NVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNL
PYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGE
YTCLAGNSIGLSHHSAWLTVLEALEERPAVMTS
PLYLEIIIYCTGAFLISCMVGSVIVYKMKSGTKK
SDFHSQMAVHKLAKSIPLRRQVTVSADSSASMN
SGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRW
ELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKP
NRVTKVAVKMLKSDATEKDLSDLISEMEMMKMI
GKHKNIINLLGACTQDGPLYVIVEYASKGNLRE
YLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAY
QVARGMEYLASKKCIHRDLAARNVLVTEDNVMK
IADFGLARDIHHIDYYKKTTNGRLPVKWMAPEA
LFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVP
VEELFKLLKEGHRMDKPSNCTNELYMMMRDCWH
AVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSM
PLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEP
CLPRHPAQLANGGLKRR*LPPTRPPQTPPSAVT
LTHSPCWAHHLSVPVPFPAGRSRLPTRGLPVWP
AFTPLSSPLPPPPLHLLVRGAKRQIFAASHFIPS
QMLDQHPSLPPGTAWRAGSGSQ*TGMQVRAS*A
FSCRFGLFCLHP*APRTLVAGALSSGLQQ*GGQ
CFVPRLKVTSAPDRWCQWLINSDTSLLC*PNAWY
QRMVRRRPGWGQCCGPGAQPQTGGSVYSYEENT
KCINLSIYLHVFLKGSLPEIYPSGKMLLVAGRH
QLLYIKNKKEKKGKCF*KGHIFFATFAVLFF*IM
F*TYFQFRSLNKNCCCF

Frame 1

PAGA*RSERSCGHRRGVLGGGRQLAGAGTPVQP
QRAEEPGCAGSWAATSGRDRDPS*RIAATSPSP
AASAPLLEKPRNPRTFLRSELGAPRRAHGTRAAV
GHAAAPGPPQGDGARSARKVRRRRCVLEEGGTR
SGDPGWRTGALPPPRLRGTSSGSIVPARAGGAE
HRAPPGVERRPRSSCDPARTRTEPGGGGPEPGTR
AHARSHKPRRTLPRRNLHAERGSV*KGGSSSLW
SIHGDVEPCHQPLTAELGCGAGSASSSGLCWSQ
PHSAPLGRPRPCLNKMLSPPRRMMMMMMTPLQRR
KKQITPNQTVCP*LHIGHPQKRWKRNCMQCRLP
RQ*SSNALPVGPQTPHCAG*KMAKNSNLTTELE
ATRSVMPPGAS*WTLWCPLTRATTPALWRMSTAA
STTHTSWMSWSGPLTGPSCKQGCPPTKQWPWVA
TWSSCVRCTVTRSRTSSG*STSR*MGARLAQTT
CLMSRS*RLLELIPPTKRWRCFT*EMSPLRTQGS
IRAWRVTLSDSPITLHG*PFWKPWKRGRQ**PR
PCTWRSSSIAQGPSSSPAWWGRSSSTR*RVVPR
RVTSTARWLCTSWPRASLCADR*QCLLTPVHP*T
LGFFWFGHHGSPPVGLPC*QGSLSMSFPKTLAG
SCLGTDWS*ANPWERAALGRWCWQRLSGWTRTN
PTV*PKWL*RC*SRTQQRKTCQT*SQKWR**R*S
GSIRISSTCWGPARRMVPCMSSWSMPPRATCGS
TCRPGGPQGWNTATTPATTQRSSSPPRTWCPAP
TRWPEAWSIWPPRSAYTETWQPGMSW*QRTM**R
*QTLASHGTFTTSTTIKRQPTADCL*SGWHPRH
YLTGSTPTRVMCGLSGCSCGRSSLWAAPHTPVC
LWRNFSSC*RRVTAWTSPVTAPTSCT**CGTAGM
QCPHRDPPSSSWWKTWTASWP*PPTRSTWTCPC
PWTSTPPAFPTPGALRAPQGRIPSSLMSRCPRS
PACPDTQPSLPMADSNAADCHPHALPRLHRQL*P
SPTAPAGPTTCPSLSPFLLAGAGCLPGAFLCGL
PSPHSAHLSLHLLSTCW*EVQRGRSLLPATSSP
PRCWTNTPPCHQALPGGQGVGANEQACK*ELPEL
SPVGLVCFAFTHKPLALWWQVPCPQGYSSREVS
ASCLD*R*PLPQIGGASGLLIPILVCFADQMPG
TRGW*GEGQVGGSVVALGPSPKLGALYIAMKKTQ
SV*I*VYIYMSF*KGRYQRFTHRVRCSWWLGGI
SCYILKTKKKKKENVFKKVIYFLLLLLFYFFKL
CSKPIFSLGPSIKIAAA

Frame 2

PPAHSARSALAATGAASSAAGGS*REPGRRCSR
SARRNPGVPGAGRPRPDGTETPRSALRRPRLPR
PRARRCLKSRGTQGLFSGPSSGRPAGRTVPVLQ
SGTPRRRGLRRAMEPGLQGK*GAAAAFWRRGAQG
LETPGGGREPSPRPASGAPAPAPLFPPGLEAPS
TERRRESSAGRGALATPPGPEQSPGAAGRSRGR
GHTPARTSHGGLSRGGTSTPSEGQFEKEDRAHCG
VSMEMWSLVTNL*LQNWDVELEVPPLLGCAGHS
HTLHR*AVPDLA*TRCSPLLGG******LLFRG
ERNR*HQTKPYARSSILDIPRKDGKEIACSAGCQ
DSEVQMPFQWDPKPHTALVEKWQRIQT*PQNWR
LQGPLCHLEHHNGLCGAL*QGQLHLHCGE*VRQ
HQPHIPAGCRGAVPSPAHPASRVARQQNSGPG*Q
RGVHV*GVQ*PAAAHPVAKAHRGEWEQDWPRQP
ALCPDLEDCWS*YHRQRDGGASLKKCLL*GRRG
VYVLGG*LYRTLPSLCMVDRSGSPGREAGSDDLA
PVPGDHHLLHRGLPHLLHGGVGHRLQDEEWYQE
E*LPQPDGCAQAGQEHPSAQTGNSVC*LQCIHE
LWGSSGSAITALLQWDSHASRGL*V*ASRRPSLG
AASGQTGLRQTPGRGLLWAGGVGRGYRAGQGQT
QPCDQSGCEDVEVGRNRERLVRPDLRNGDDEDD
REA*EYHQPAGGLHAGWSLVCHRGVCLQGQPAGV
PAGPEAPRAGILLQPQPQPRGAALLQGPGVLRL
PGGPRHGVSGLQEVHTPRPGSQECPGDRGQCDE
DSRLWPRTGHSPHRLL*KDNQRPTACEVDGTRGI
I*PDLHPPE*CVVFRGAPVGDLHSGRLPIPRCA
CGGTFQAAEGGSPHGQAQ*LHQRAVHDDAGLLA
CSALTETHLQAAGGRPGPHRGLDLQPGVPGPVHA
PGPVLPQLSRHPELYVLLRGGFRLLS*AAARGA
LPAPTPSPACQWRTQTPLTATHTPSPDSTVSCN
PHPQPLLGPPPVRPCPLSCWQEPAAYQGPSCVAC
LHPTQLTSPSTSSPPAGERCKEADLCCQPLHPL
PDVGPTPLPATRHCLEGREWEPMNRHASESFLS
FLLSVWSVLPSPISPSHSGGRCLVLRATAVGRSV
LRASIEGDLCPR*VVPVAY*FRY*FALLTKCLV
PEDGEAKARLGAVLWPWGPAPNWGLCI*L*RKH
KVYKSEYIFTCLFKRVVTRDLPIG*DAPGGWEAS
VAIY*KQKRKKRKMFLKRSYIFCYFCCFIFLNY
VLNLFSV*VPQ*KLLLL

Sequence FGFR1.iAug05

CCTCCAACTCGCGCTCGGGAGCTGGCGAGGCGGCGGCGGCTCCTCAGGTCAGTTTGAAAAGGAGGATCGAGCTCACTGTGGAGTATCCATGGAGATGTGG
AGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGAGCTGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGCCACACTCTGCACCGCTAGG
CCGTCCCCGACCTTGCCTGAACAAGCCCAGCCCTGGGGAGCCCCTGTGGAAGTGGAGTCCTTCCTGGTCCACCCCGGTGACCTGCTGCAGCTTCGCTGTC
GGCTGCGGGACGATGTGCAGAGCATCAACTGGCTGCGGGACGGGGTGCAGCTGGCGGAAAGCAACCGCACCCGCATCACAGGGGAGGAGGTGGAGGTGCA
GGACTCCGTGCCCGCAGACTCCGGCCTCTATGCTTGCGTAACCAGCAGCCCCTCGGGCAGTGACACCACCTACTTCTCCGTCAATGTTTCAGATGCTCTC
CCCTCCTCGGAGGATGATGATGATGATGATGACTCCTCTTCAGAGGAGAAAGAAACAGATAACACCAAACCAAACCCCGTAGCTCCATATTGGACATCCC
CAGAAAAGATGGAAAAGAAATTGCATGCAGTGCCGGCTGCCAAGACAGTGAAGTTCAAATGCCCTTCCAGTGGGACCCCAAACCCCACACTGCGCTGGTT
GAAAAATGGCAAAGAATTCAAACCTGACCACAGAATTGGAGGCTACAAGGTCCGTTATGCCACCTGGAGCATCATAATGGACTCTGTGGTGCCCTCTGAC
AAGGGCAACTACACCTGCATTGTGGAGAATGAGTACGGCAGCATCAACCACACATACCAGCTGGATGTCGTGGAGCGGTCCCCTCACCGGCCCATCCTGC
AAGCAGGGTTGCCCGCCAACAAAACAGTGGCCCTGGGTAGCAACGTGGAGTTCATGTGTAAGGTGTACAGTGACCCGCAGCCGCACATCCAGTGGCTAAA
GCACATCGAGGTGAATGGGAGCAAGATTGGCCCAGACAACCTGCCTTATGTCCAGATCTTGAAGCATTCGGGGATTAATAGCTCGGATGCGGAGGTGCTG
ACCCTGTTCAATGTGACAGAGGCCCAGAGCGGGGAGTATGTGTGTAAGGTTTCCAATTATATTGGTGAAGCTAACCAGTCTGCGTGGCTCACTGTCACCA
GACCTGTGGCAAAAGCCCTGGAAGAGAGGCCGGCAGTGATGACCTCGCCCCTGTACCTGGAGATCATCATCTATTGCACAGGGGCCTTCCTCATCTCCTG
CATGGTGGGGTCGGTCATCGTCTACAAGATGAAGAGTGGTACCAAGAAGAGTGACTTCCACAGCCAGATGGCTGTGCACAAGCTGGCCAAGAGCATCCCT
CTGCGCAGACAGGTAACAGTGTCTGCTGACTCCAGTGCATCCATGAACTCTGGGGTTCTTCTGGTTCGGCCATCACGGCTCTCCTCCAGTGGGACTCCCA
TGCTAGCAGGGGTCTCTGAGTATGAGCTTCCCGAAGACCCTCGCTGGGAGCTGCCTCGGGACAGGTAATAATACTTTGACTCCCAACTTTTTGAAGGCAT
TCCTTGCTTGCTATTCTAGTGGTGGAAGGTGTGTATACTAACACCTTCTGGGCGGTGAAGGTTCATGAAATGCCTGCAGAAACATTCATTCCACTTTCTG
CTCGCAGTTGGGTGAAATACAGAGAGGTGGAGATGGGGTGCCTCTCAAGGTTTGAACTTCACCAGCCCCAACTTATGCCACTCTCTGTTTCCCCCGAAAG
ACTGGTCTTAGGCAAACCCCTGGGAGAGGGCTGCTTTGGGCAGGTGGTGTTGGCAGAGGCTATCGGGCTGGACAAGGACAAACCCAACCGTGTGACCAAA
GTGGCTGTGAAGATGTTGAAGTGTAAGTGATGCTTCTGTCCTTGCAAAGAAAATCTTGTCCCATTCCAAGCAAACAGCAGGCCTCGGGCCCAGCAGAACA
GCTTCTCTGGTGCCTTTGCTGCCCACACCTGCTCCGCTGCCTGCTTGGGGTTACCTGGGAGTGGAGGGGGTGTTCTGCACCTTGAGATTGTTCTATGACG
TCACTCTGTTCCTTGGCGTCAACCTGGGCTGTGGTCAGGTGTTTATCCTGGATCCATCGCTTGCAGGCTTTCCTCACCACATCCCTGCCCACTCACTACC
ACCTATTGCAACAACGGCTCCCATGAGAGGGAAGAGAAAACAAGTTTGGATGAAGTGGGGAGGAGAGAATCAAGTCCCAGGGAAAAGCAGCCCCTCGACA
CATACCCCACTCCCTTAGCCTTTATCCTGCCCTCCTCCCTTCCCAAGTAAATGAGTCTCAACGTGTTCTTTAAAGCGGACGCAACAGAGAAAGACTTGTC
AGACCTGATCTCAGAAATGGAGATGATGAAGATGATCGGGAAGCATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTGGGTGCCGGCCA
GACTGGCTTTCCCTGCCCAGCGGGGAGAAGATCTGAGCTGGGGTATCAGTCCCAAGGCCTCTCTCAGCCACAGCTAGGGAAGGGGGTTAAGAGAGGCTGC
TTTGGCAGAGGTTTAAAATGTGTTATCTCGGGATCTGGGCTCCGCTCATTGGTGTTCATTCAGGAATACAGATACCTAGATTCACACACGTGGTGTGTTA
ACAGAGGCAGCTGAGTAATTATGGTCTAAGAGCGCTCAGGCTGGGTGCAGTGGCTCATGCCTGTAATCCCAGCAATTTGGGAGGCCGAGGCAGGAAGATC
ACTTGAGATCAGGAGTTCGAGACCAGTCTGGTCAAAATGGTGAAACCCCATCTCTACTAAAAAATACAAAAGTTAGCCAGGCATGGTCACGCATGCCTAT
AATCCCAGCTACTCAGGAGGCTGACGCATAAGACTTGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCACGCCATTGTACTCCAGCTTGGGA
GATACTCCCGCTGTCTCAAAAATAAAATAAATAAATAGGCCAGGCACAGTGGCTCACGCCTGTAATCCCAGCACCTTGGGAGGGTGAGGCGGGTGGATCA
CTTGAGGTCAGGAGTTGGAGACCAGCCTGGCCAACAGGTGAAACCCCATCTCTACTAAAAATACAAAAAGTAGCCGGGCGTGGTGGCGCATGCCTGTAAT
TCCAGCTACTCGGGAGGCTGTGGCAGGAGAATCGTTTGAACACGGGAGGCGGAGGTTGCGGTTAGCCGAGATCGCGCCATTGCACTCCAGCTTGGGCAAC
GAGCAAAACTCCATCTC
  • FGFR1.F11 (forward primer)
  • FGFR1.R11 (reverse primer)
  • Single exon skipping

Frame 0

PPTRARELARRRRLLRSV*KGGSSSLWSIHGDV
EPCHQPLTAELGCGAGSASSSGLCWSQPHSAPLG
RPRPCLNKPSPGEPLWKWSPSWSTPVTCCSFAV
GCGTMCRASTGCGTGCSWRKATAPASQGRRWRC
RTPCPQTPASMLA*PAAPRAVTPPTSPSMFQMLS
PPRRMMMMMMTPLQRRKKQITPNQTP*LHIGHP
QKRWKRNCMQCRLPRQ*SSNALPVGPQTPHCAG
*KMAKNSNLTTELEATRSVMPPGAS*WTLWCPLT
RATTPALWRMSTAASTTHTSWMSWSGPLTGPSC
KQGCPPTKQWPWVATWSSCVRCTVTRSRTSSG*
STSR*MGARLAQTTCLMSRS*SIRGLIARMRRC*
PCSM*QRPRAGSMCVRFPIILVKLTSLRGSLSP
DLWQKPWKRGRQ**PRPCTWRSSSIAQGPSSSP
AWWGRSSSTR*RVVPRRVTSTARWLCTSWPRASL
CADR*QCLLTPVHP*TLGFFWFGHHGSPPVGLP
C*QGSLSMSFPKTLAGSCLGTGNNTLTPNFLKA
FLACYSSGGRCVY*HLLGGEGS*NACRNIHSTFC
SQLGEIQRGGDGVPLKV*TSPAPTYATLCFPRK
TGLRQTPGRGLLWAGGVGRGYRAGQGQTQPCDQ
SGCEDVEV*VMLLSLQRKSCPIPSKQQASGPAEQ
LLWCLCCPHLLRCLLGVTWEWRGCSAP*DCSMT
SLCSLASTWAVVRCLSWIHRLQAFLTTSLPTHY
HLLQQRLP*EGRENKFG*SGEERIKSQGKAAPRH
IPHSLSLYPALLPSQVNESQRVL*SGRNRERLV
RPDLRNGDDEDDREA*EYHQPAGGLHAGWWVPA
RLAFPAQRGEDLSWGISPKASLSHS*GRGLREAA
LAEV*NVLSRDLGSAHWCSFRNTDT*IHTRGVL
TEAAE*LWSKSAQAGCSGSCL*SQQFGRPRQED
HLRSGVRDQSGQNGETPSLLKNTKVSQAWSRMPI
IPATQEADA*DLLEPGRWRLQ*AKITPLYSSLG
DTPAVSKIK*INRPGTVAHACNPSTLGG*GGWI
T*GQELETSLANR*NPISTKNTKSSRAWWRMPVI
PATREAVAGESFEHGRRRLRLAEIAPLHSSLGN
EQNSI

Frame 1

LQLALGSWRGGGGSSGQFEKEDRAHCGVSMEMW
SLVTNL*LQNWDVELEVPPLLGCAGHSHTLHR*
AVPDLA*TSPALGSPCGSGVLPGPPR*PAAASLS
AAGRCAEHQLAAGRGAAGGKQPHPHHRGGGGGA
GLRARRLRPLCLRNQQPLGQ*HHLLLRQCFRCS
PLLGG******LLFRGERNR*HQTKPRSSILDIP
RKDGKEIACSAGCQDSEVQMPFQWDPKPHTALV
EKWQRIQT*PQNWRLQGPLCHLEHHNGLCGAL*
QGQLHLHCGE*VRQHQPHIPAGCRGAVPSPAHPA
SRVARQQNSGPG*QRGVHV*GVQ*PAAAHPVAK
AHRGEWEQDWPRQPALCPDLEAFGD**LGCGGA
DPVQCDRGPERGVCV*GFQLYW*S*PVCVAHCHQ
TCGKSPGREAGSDDLAPVPGDHHLLHRGLPHLL
HGGVGHRLQDEEWYQEE*LPQPDGCAQAGQEHP
SAQTGNSVC*LQCIHELWGSSGSAITALLQWDSH
ASRGL*V*ASRRPSLGAASGQVIIL*LPTF*RH
SLLAILVVEGVYTNTFWAVKVHEMPAETFIPLS
ARSWVKYREVEMGCLSRFELHQPQLMPLSVSPER
LVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTK
VAVKMLKCK*CFCPCKENLVPFQANSRPRAQQN
SFSGAFAAHTCSAACLGLPGSGGGVLHLEIVL*R
HSVPWRQPGLWSGVYPGSIACRLSSPHPCPLTT
TYCNNGSHEREEKTSLDEVGRRESSPREKQPLD
TYPTPLAFILPSSLPK*MSLNVFFKADATEKDLS
DLISEMEMMKMIGKHKNIINLLGACTQDGGCRP
DWLSLPSGEKI*AGVSVPRPLSATAREGG*ERL
LWQRFKMCYLGIWAPLIGVHSGIQIPRFTHVVC*
QRQLSNYGLRALRLGAVAHACNPSNLGGRGRKI
T*DQEFETSLVKMVKPHLY*KIQKLARHGHACL
*SQLLRRLTHKTCLNPGGGGCSEPRSRHCTPAWE
ILPLSQK*NK*IGQAQWLTPVIPAPWEGEAGGS
LEVRSWRPAWPTGETPSLLKIQKVAGRGGACL*
FQLLGRLWQENRLNTGGGGCG*PRSRHCTPAWAT
SKTPS

Frame 2

SNSRSGAGEAAAAPQVSLKRRIELTVEYPWRCG
ALSPTSNCRTGMWSWKCLLFWAVLVTATLCTAR
PSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRC
RLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQ
DSVPADSGLYACVTSSPSGSDTTYFSVNVSDAL
PSSEDDDDDDDSSSEEKETDNTKPNPVAPYWTS
PEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWL
KNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSD
KGNYTCIVENEYGSINHTYQLDVVERSPHRPIL
QAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLK
HIEVNGSKIGPDNLPYVQILKHSGINSSDAEVL
TLFNVTEAQSGEYVCKVSNYIGEANQSAWLTVT
RPVAKALEERPAVMTSPLYLEIIIYCTGAFLISC
MVGSVIVYKMKSGTKKSDFHSQMAVHKLAKSIP
LRRQVTVSADSSASMNSGVLLVRPSRLSSSGTP
MLAGVSEYELPEDPRWELPRDR**YFDSQLFEGI
PCLLF*WWKVCILTPSGR*RFMKCLQKHSFHFL
LAVG*NTERWRWGASQGLNFTSPNLCHSLFPPK
DWS*ANPWERAALGRWCWQRLSGWTRTNPTV*PK
WL*RC*SVSDASVLAKKILSHSKQTAGLGPSRT
ASLVPLLPTPAPLPAWGYLGVEGVFCTLRLFYD
VTLFLGVNLGCGQVFILDPSLAGFPHHIPAHSLP
PIATTAPMRGKRKQVWMKWGGENQVPGKSSPST
HTPLP*PLSCPPPFPSK*VSTCSLKRTQQRKTC
QT*SQKWR**R*SGSIRISSTCWGPARRMVGAGQ
TGFPCPAGRRSELGYQSQGLSQPQLGKGVKRGC
FGRGLKCVISGSGLRSLVFIQEYRYLDSHTWCV
NRGS*VIMV*ERSGWVQWLMPVIPAIWEAEAGRS
LEIRSSRPVWSKW*NPISTKKYKS*PGMVTHAY
NPSYSGG*RIRLA*TREVEVAVSQDHAIVLQLG
RYSRCLKNKINK*ARHSGSRL*SQHLGRVRRVDH
LRSGVGDQPGQQVKPHLY*KYKK*PGVVAHACN
SSYSGGCGRRIV*TREAEVAVSRDRAIALQLGQ
RAKLHL

Sequence FGFR1.fAug05

CCCCGCCGGCGCATAGCGCTCGGAGCGCTCTTGCGGCCACAGGCGCGGCGTCCTCGGCGGCGGGCGGCAGCTAGCGGGAGCCGGGACGCCGGTGCAGCCG
CAGCGCGCGGAGGAACCCGGGTGTGCCGGGAGCTGGGCGGCCACGTCCGGACGGGACCGAGACCCCTCGTAGCGCATTGCGGCGACCTCGCCTTCCCCGG
CCGCGAGCGCGCCGCTGCTTGAAAAGCCGCGGAACCCAAGGACTTTTCTCCGGTCCGAGCTCGGGGCGCCCCGCAGGGCGCACGGTACCCGTGCTGCAGT
CGGGCACGCCGCGGCGCCGGGGCCTCCGCAGGGCGATGGAGCCCGGTCTGCAAGGAAAGTGAGGCGCCGCCGCTGCGTTCTGGAGGAGGGGGGCACAAGG
TCTGGAGACCCCGGGTGGCGGACGGGAGCCCTCCCCCCGCCCCGCCTCCGGGGCACCAGCTCCGGCTCCATTGTTCCCGCCCGGGCTGGAGGCGCCGAGC
ACCGAGCGCCGCCGGGAGTCGAGCGCCGGCCGCGGAGCTCTTGCGACCCCGCCAGGACCCGAACAGAGCCCGGGGGCGGCGGGCCGGAGCCGGGGACGCG
GGCACACGCCCGCTCGCACAAGCCACGGCGGACTCTCCCGAGGCGGAACCTCCACGCCGAGCGAGGGTCAGTTTGAAAAGGAGGATCGAGCTCACTGTGG
AGTATCCATGGAGATGTGGAGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGAGCTGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGC
CACACTCTGCACCGCTAGGCCGTCCCCGACCTTGCCTGAACAAGCCCAGCCCTGGGGAGCCCCTGTGGAAGTGGAGTCCTTCCTGGTCCACCCCGGTGAC
CTGCTGCAGCTTCGCTGTCGGCTGCGGGACGATGTGCAGAGCATCAACTGGCTGCGGGACGGGGTGCAGCTGGCGGAAAGCAACCGCACCCGCATCACAG
GGGAGGAGGTGGAGGTGCAGGACTCCGTGCCCGCAGACTCCGGCCTCTATGCTTGCGTAACCAGCAGCCCCTCGGGCAGTGACACCACCTACTTCTCCGT
CAATGTTTCAGATGCTCTCCCCTCCTCGGAGGATGATGATGATGATGATGACTCCTCTTCAGAGGAGAAAGAAACAGATAACACCAAACCAAACCGTATG
CCCGTAGCTCCATATTGGACATCCCCAGAAAAGATGGAAAAGAAATTGCATGCAGTGCCGGCTGCCAAGACAGTGAAGTTCAAATGCCCTTCCAGTGGGA
CCCCAAACCCCACACTGCGCTGGTTGAAAAATGGCAAAGAATTCAAACCTGACCACAGAATTGGAGGCTACAAGGTCCGTTATGCCACCTGGAGCATCAT
AATGGACTCTGTGGTGCCCTCTGACAAGGGCAACTACACCTGCATTGTGGAGAATGAGTACGGCAGCATCAACCACACATACCAGCTGGATGTCGTGGAG
CGGTCCCCTCACCGGCCCATCCTGCAAGCAGGGTTGCCCGCCAACAAAACAGTGGCCCTGGGTAGCAACGTGGAGTTCATGTGTAAGGTGTACAGTGACC
CGCAGCCGCACATCCAGTGGCTAAAGCACATCGAGGTGAATGGGAGCAAGATTGGCCCAGACAACCTGCCTTATGTCCAGATCTTGAAGACTGCTGGAGT
TAATACCACCGACAAAGAGATGGAGGTGCTTCACTTAAGAAATGTCTCCTTTGAGGACGCAGGGGAGTATACGTGCTTGGCGGGTAACTCTATCGGACTC
TCCCATCACTCTGCATGGTTGACCGTTCTGGAAGCCCTGGAAGAGAGGCCGGCAGTGATGACCTCGCCCCTGTACCTGGAGATCATCATCTATTGCACAG
GGGCCTTCCTCATCTCCTGCATGGTGGGGTCGGTCATCGTCTACAAGATGAAGAGTGGTACCAAGAAGAGTGACTTCCACAGCCAGATGGCTGTGCACAA
GCTGGCCAAGAGCATCCCTCTGCGCAGACAGGTAACAGTGTCTGCTGACTCCAGTGCATCCATGAACTCTGGGGTTCTTCTGGTTCGGCCATCACGGCTC
TCCTCCAGTGGGACTCCCATGCTAGCAGGGGTCTCTGAGTATGAGCTTCCCGAAGACCCTCGCTGGGAGCTGCCTCGGGACAGACTGGTCTTAGGCAAAC
CCCTGGGAGAGGGCTGCTTTGGGCAGGTGGTGTTGGCAGAGGCTATCGGGCTGGACAAGGACAAACCCAACCGTGTGACCAAAGTGGCTGTGAAGATGTT
GAAGTCGGACGCAACAGAGAAAGACTTGTCAGACCTGATCTCAGAAATGGAGATGATGAAGATGATCGGGAAGCATAAGAATATCATCAACCTGCTGGGG
GCCTGCACGCAGGATGGTCCCTTGTATGTCATCGTGGAGTATGCCTCCAAGGGCAACCTGCGGGAGTACCTGCAGGCCCGGAGGCCCCCAGGGCTGGAAT
ACTGCTACAACCCCAGCCACAACCCAGAGGAGCAGCTCTCCTCCAAGGACCTGGTGTCCTGCGCCTACCAGGTGGCCCGAGGCATGGAGTATCTGGCCTC
CAAGAAGGTGTGGAACCTGAAGGCTCCCCTGGTGCATACACCGAGACCTGGCAGCCAGGAATGTCCTGGTGACAGAGGACAATGTGATGAAGATAGCAGA
CTTTGGCCTCGCACGGGACATTCACCACATCGACTACTATAAAAAGACAACCAACGGCCGACTGCCTGTGAAGTGGATGGCACCCGAGGCATTATTTGAC
CGGATCTACACCCACCAGAGTGATGTGTGGTCTTTCGGGGTGCTCCTGTGGGAGATCTTCACTCTGGGCGGCTCCCCATACCCCGGTGTGCCTGTGGAGG
AACTTTTCAAGCTGCTGAAGGAGGGTCACCGCATGGACAAGCCCAGTAACTGCACCAACGAGCTGTACATGATGATGCGGGACTGCTGGCATGCAGTGCC
CTCACAGAGACCCACCTTCAAGCAGCTGGTGGAAGACCTGGACCGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCCCCTGGAC
CAGTACTCCCCCAGCTTTCCCGACACCCGGAGCTCTACGTGCTCCTCAGGGGAGGATTCCGTCTTCTCTCATGAGCCGCTGCCCGAGGAGCCCTGCCTGC
CCCGACACCCAGCCCAGCTTGCCAATGGCGGACTCAAACGCCGCTGACTGCCACCCACACGCCCTCCCCAGACTCCACCGTCAGCTGTAACCCTCACCCA
CAGCCCCTGCTGGGCCCACCACCTGTCCGTCCCTGTCCCCTTTCCTGCTGGCAGGAGCCGGCTGCCTACCAGGGGCCTTCCTGTGTGGCCTGCCTTCACC
CCACTCAGCTCACCTCTCCCTCCACCTCCTCTCCACCTGCTGGTGAGAGGTGCAAAGAGGCAGATCTTTGCTGCCAGCCACTTCATCCCCTCCCAGATGT
TGGACCAACACCCCTCCCTGCCACCAGGCACTGCCTGGAGGGCAGGGAGTGGGAGCCAATGAACAGGCATGCAAGTGAGAGCTTCCTGAGCTTTCTCCTG
TCGGTTTGGTCTGTTTTGCCTTCACCCATAAGCCCCTCGCACTCTGGTGGCAGGTGCCTTGTCCTCAGGGCTACAGCAGTAGGGAGGTCAGTGCTTCGTG
CCTCGATTGAAGGTGACCTCTGCCCCAGATAGGTGGTGCCAGTGGCTTATTAATTCCGATACTAGTTTGCTTTGCTGACCAAATGCCTGGTACCAGAGGA
TGGTGAGGCGAAGGCCAGGTTGGGGGCAGTGTTGTGGCCCTGGGGCCCAGCCCCAAACTGGGGGCTCTGTATATAGCTATGAAGAAAACACAAAGTGTAT
AAATCTGAGTATATATTTACATGTCTTTTTAAAAGGGTCGTTACCAGAGATTTACCCATCGGGTAAGATGCTCCTGGTGGCTGGGAGGCATCAGTTGCTA
TATATTAAAAACAAAAAAGAAAAAAAAGGAAAATGTTTTTAAAAAGGTCATATATTTTTTGCTACTTTTGCTGTTTTATTTTTTTAAATTATGTTCTAAA
CCTATTTTCAGTTTAGGTCCCTCAATAAAAATTGCTGCTGCTTC
  • FGFR1.F11 (forward primer)
  • FGFR1.R11 (reverse primer)
  • Single exon skipping

Frame 0

PRRRIALGALLRPQARRPRRRAAASGSRDAGAA
AARGGTRVCRELGGHVRTGPRPLVAHCGDLAFPG
RERAAA*KAAEPKDFSPVRARGAPQGARYPCCS
RARRGAGASAGRWSPVCKESEAPPLRSGGGGHK
VWRPRVADGSPPPAPPPGHQLRLHCSRPGWRRRA
PSAAGSRAPAAELLRPRQDPNRARGRRAGAGDA
GTRPLAQATADSPEAEPPRRARVSLKRRIELTV
EYPWRCGALSPTSNCRTGMWSWKCLLFWAVLVTA
TLCTARPSPTLPEQAQPWGAPVEVESFLVHPGD
LLQLRCRLRDDVQSINWLRDGVQLAESNRTRIT
GEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSV
NVSDALPSSEDDDDDDDSSSEEKETDNTKPNRM
PVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSG
TPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSII
MDSVVPSDKGNYTCIVENEYGSINHTYQLDVVE
RSPHRPILQAGLPANKTVALGSNVEFMCKVYSD
PQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGV
NTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGL
SHHSAWLTVLEALEERPAVMTSPLYLEIIIYCT
GAFLISCMVGSVIVYKMKSGTKKSDFHSQMAVHK
LAKSIPLRRQVTVSADSSASMNSGVLLVRPSRL
SSSGTPMLAGVSEYELPEDPRWELPRDRLVLGK
PLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKML
KSDATEKDLSDLISEMEMMKMIGKHKNIINLLG
ACTQDGPLYVIVEYASKGNLREYLQARRPPGLE
YCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLAS
KKVWNLKAPLVHTPRPGSQECPGDRGQCDEDSR
LWPRTGHSPHRLL*KDNQRPTACEVDGTRGII*
PDLHPPE*CVVFRGAPVGDLHSGRLPIPRCACGG
TFQAAEGGSPHGQAQ*LHQRAVHDDAGLLACSA
LTETHLQAAGGRPGPHRGLDLQPGVPGPVHAPG
PVLPQLSRHPELYVLLRGGFRLLS*AAARGALPA
PTPSPACQWRTQTPLTATHTPSPDSTVSCNPHP
QPLLGPPPVRPCPLSCWQEPAAYQGPSCVACLH
PTQLTSPSTSSPPAGERCKEADLCCQPLHPLPDV
GPTPLPATRHCLEGREWEPMNRHASESFLSFLL
SVWSVLPSPISPSHSGGRCLVLRATAVGRSVLR
ASIEGDLCPR*VVPVAY*FRY*FALLTKCLVPED
GEAKARLGAVLWPWGPAPNWGLCI*L*RKHKVY
KSEYIFTCLFKRVVTRDLPIG*DAPGGWEASVA
IY*KQKRKKRKMFLKRSYIFCYFCCFIFLNYVLN
LFSV*VPQ*KLLLL

Frame 1

PAGA*RSERSCGHRRGVLGGGRQLAGAGTPVQP
QRAEEPGCAGSWAATSGRDRDPS*RIAATSPSP
AASAPLLEKPRNPRTFLRSELGAPRRAHGTRAAV
GHAAAPGPPQGDGARSARKVRRRRCVLEEGGTR
SGDPGWRTGALPPPRLRGTSSGSIVPARAGGAE
HRAPPGVERRPRSSCDPARTRTEPGGGGPEPGTR
AHARSHKPRRTLPRRNLHAERGSV*KGGSSSLW
SIHGDVEPCHQPLTAELGCGAGSASSSGLCWSQ
PHSAPLGRPRPCLNKPSPGEPLWKWSPSWSTPVT
CCSFAVGCGTMCRASTGCGTGCSWRKATAPASQ
GRRWRCRTPCPQTPASMLA*PAAPRAVTPPTSP
SMFQMLSPPRRMMMMMMTPLQRRKKQITPNQTVC
P*LHIGHPQKRWKRNCMQCRLPRQ*SSNALPVG
PQTPHCAG*KMAKNSNLTTELEATRSVMPPGAS
*WTLWCPLTRATTPALWRMSTAASTTHTSWMSWS
GPLTGPSCKQGCPPTKQWPWVATWSSCVRCTVT
RSRTSSG*STSR*MGARLAQTTCLMSRS*RLLE
LIPPTKRWRCFT*EMSPLRTQGSIRAWRVTLSDS
PITLHG*PFWKPWKRGRQ**PRPCTWRSSSIAQ
GPSSSPAWWGRSSSTR*RVVPRRVTSTARWLCT
SWPRASLCADR*QCLLTPVHP*TLGFFWFGHHGS
PPVGLPC*QGSLSMSFPKTLAGSCLGTDWS*AN
PWERAALGRWCWQRLSGWTRTNPTV*PKWL*RC
*SRTQQRKTCQT*SQKWR**R*SGSIRISSTCWG
PARRMVPCMSSWSMPPRATCGSTCRPGGPQGWN
TATTPATTQRSSSPPRTWCPAPTRWPEAWSIWP
PRRCGT*RLPWCIHRDLAARNVLVTEDNVMKIAD
FGLARDIHHIDYYKKTTNGRLPVKWMAPEALFD
RIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVE
ELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVP
SQRPTFKQLVEDLDRIVALTSNQEYLDLSMPLD
QYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCL
PRHPAQLANGGLKRR*LPPTRPPQTPPSAVTLTH
SPCWAHHLSVPVPFPAGRSRLPTRGLPVWPAFT
PLSSPLPPPPLHLLVRGAKRQIFAASHFIPSQM
LDQHPSLPPGTAWRAGSGSQ*TGMQVRAS*AFSC
RFGLFCLHP*APRTLVAGALSSGLQQ*GGQCFV
PRLKVTSAPDRWCQWLINSDTSLLC*PNAWYQR
MVRRRPGWGQCCGPGAQPQTGGSVYSYEENTKCI
NLSIYLHVFLKGSLPEIYPSGKMLLVAGRHQLL
YIKNKKEKKGKCF*KGHIFFATFAVLFF*IMF*
TYFQFRSLNKNCCCF

Frame 2

PPAHSARSALAATGAASSAAGGS*REPGRRCSR
SARRNPGVPGAGRPRPDGTETPRSALRRPRLPR
PRARRCLKSRGTQGLFSGPSSGRPAGRTVPVLQ
SGTPRRRGLRRAMEPGLQGK*GAAAAFWRRGAQG
LETPGGGREPSPRPASGAPAPAPLFPPGLEAPS
TERRRESSAGRGALATPPGPEQSPGAAGRSRGR
GHTPARTSHGGLSRGGTSTPSEGQFEKEDRAHCG
VSMEMWSLVTNL*LQNWDVELEVPPLLGCAGHS
HTLHR*AVPDLA*TSPALGSPCGSGVLPGPPR*
PAAASLSAAGRCAEHQLAAGRGAAGGKQPHPHHR
GGGGGAGLRARRLRPLCLRNQQPLGQ*HHLLLR
QCFRCSPLLGG******LLFRGERNR*HQTKPY
ARSSILDIPRKDGKEIACSAGCQDSEVQMPFQWD
PKPHTALVEKWQRIQT*PQNWRLQGPLCHLEHH
NGLCGAL*QGQLHLHCGE*VRQHQPHIPAGCRG
AVPSPAHPASRVARQQNSGPG*QRGVHV*GVQ*P
AAAHPVAKAHRGEWEQDWPRQPALCPDLEDCWS
*YHRQRDGGASLKKCLL*GRRGVYVLGG*LYRT
LPSLCMVDRSGSPGREAGSDDLAPVPGDHHLLHR
GLPHLLHGGVGHRLQDEEWYQEE*LPQPDGCAQ
AGQEHPSAQTGNSVC*LQCIHELWGSSGSAITA
LLQWDSHASRGL*V*ASRRPSLGAASGQTGLRQT
PGRGLLWAGGVGRGYRAGQGQTQPCDQSGCEDV
EVGRNRERLVRPDLRNGDDEDDREA*EYHQPAG
GLHAGWSLVCHRGVCLQGQPAGVPAGPEAPRAGI
LLQPQPQPRGAALLQGPGVLRLPGGPRHGVSGL
QEGVEPEGSPGAYTETWQPGMSW*QRTM**R*Q
TLASHGTFTTSTTIKRQPTADCL*SGWHPRHYLT
GSTPTRVMCGLSGCSCGRSSLWAAPHTPVCLWR
NFSSC*RRVTAWTSPVTAPTSCT**CGTAGMQC
PHRDPPSSSWWKTWTASWP*PPTRSTWTCPCPWT
STPPAFPTPGALRAPQGRIPSSLMSRCPRSPAC
PDTQPSLPMADSNAADCHPHALPRLHRQL*PSP
TAPAGPTTCPSLSPFLLAGAGCLPGAFLCGLPSP
HSAHLSLHLLSTCW*EVQRGRSLLPATSSPPRC
WTNTPPCHQALPGGQGVGANEQACK*ELPELSP
VGLVCFAFTHKPLALWWQVPCPQGYSSREVSASC
LD*R*PLPQIGGASGLLIPILVCFADQMPGTRG
W*GEGQVGGSVVALGPSPKLGALYIAMKKTQSV
*I*VYIYMSF*KGRYQRFTHRVRCSWWLGGISCY
ILKTKKKKKENVFKKVIYFLLLLLFYFFKLCSK
PIFSLGPSIKIAAA

Sequence FGFR1.cAug05

CCCCGCCGGCGCATAGCGCTCGGAGCGCTCTTGCGGCCACAGGCGCGGCGTCCTCGGCGGCGGGCGGCAGCTAGCGGGAGCCGGGACGCCGGTGCAGCCG
CAGCGCGCGGAGGAACCCGGGTGTGCCGGGAGCTGGGCGGCCACGTCCGGACGGGACCGAGACCCCTCGTAGCGCATTGCGGCGACCTCGCCTTCCCCGG
CCGCGAGCGCGCCGCTGCTTGAAAAGCCGCGGAACCCAAGGACTTTTCTCCGGTCCGAGCTCGGGGCGCCCCGCAGGGCGCACGGTACCCGTGCTGCAGT
CGGGCACGCCGCGGCGCCGGGGCCTCCGCAGGGCGATGGAGCCCGGTCTGCAAGGAAAGTGAGGCGCCGCCGCTGCGTTCTGGAGGAGGGGGGCACAAGC
TCCGGCTCCATTGTTCCCGCCCGGGCTGGAGGCGCCGAGCACCGAGCGCCGCCGGGAGTCGAGCGCCGGCCGCGGAGCTCTTGCGACCCCGCCAGGACCC
GAACAGAGCCCGGGGGCGGCGGGCCGGAGCCGGGGACGCGGGCACACGCCCGCTCGCACAAGCCACGGCGGACTCTCCCGAGGCGGAACCTCCACGCCGA
GCGAGGGTCAGTTTGAAAAGGAGGATCGAGCTCACTGTGGAGTATCCATGGAGATGTGGAGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGA
GCTGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGCCACACTCTGCACCGCTAGGCCGTCCCCGACCTTGCCTGAACAAGCCCAGCCCTGGGGAGC
CCCTGTGGAAGTGGAGTCCTTCCTGGTCCACCCCGGTGACCTGCTGCAGCTTCGCTGTCGGCTGCGGGACGATGTGCAGAGCATCAACTGGCTGCGGGAC
GGGGTGCAGCTGGCGGAAAGCAACCGCACCCGCATCACAGGGGAGGAGGTGGAGGTGCAGGACTCCGTGCCCGCAGACTCCGGCCTCTATGCTTGCGTAA
CCAGCAGCCCCTCGGGCAGTGACACCACCTACTTCTCCGTCAATGTTTCAGATGCTCTCCCCTCCTCGGAGGATGATGATGATGATGATGACTCCTCTTC
AGAGGAGAAAGAAACAGATAACACCAAACCAAACCGTATGCCCGTAGCTCCATATTGGACATCCCCAGAAAAGATGGAAAAGAAATTGCATGCAGTGCCG
GCTGCCAAGACAGTGAAGTTCAAATGCCCTTCCAGTGGGACCCCAAACCCCACACTGCGCTGGTTGAAAAATGGCAAAGAATTCAAACCTGACCACAGAA
TTGGAGGCTACAAGGTCCGTTATGCCACCTGGAGCATCATAATGGACTCTGTGGTGCCCTCTGACAAGGGCAACTACACCTGCATTGTGGAGAATGAGTA
CGGCAGCATCAACCACACATACCAGCTGGATGTCGTGGAGCGGTCCCCTCACCGGCCCATCCTGCAAGCAGGGTTGCCCGCCAACAAAACAGTGGCCCTG
GGTAGCAACGTGGAGTTCATGTGTAAGGTGTACAGTGACCCGCAGCCGCACATCCAGTGGCTAAAGCACATCGAGGTGAATGGGAGCAAGATTGGCCCAG
ACAACCTGCCTTATGTCCAGATCTTGAAGACTGCTGGAGTTAATACCACCGACAAAGAGATGGAGGTGCTTCACTTAAGAAATGTCTCCTTTGAGGACGC
AGGGGAGTATACGTGCTTGGCGGGTAACTCTATCGGACTCTCCCATCACTCTGCATGGTTGACCGTTCTGGAAGCCCTGGAAGAGAGGCCGGCAGTGATG
ACCTCGCCCCTGTACCTGGAGATCATCATCTATTGCACAGGGGCCTTCCTCATCTCCTGCATGGTGGGGTCGGTCATCGTCTACAAGATGAAGAGTGGTA
CCAAGAAGAGTGACTTCCACAGCCAGATGGCTGTGCACAAGCTGGCCAAGAGCATCCCTCTGCGCAGACAGGTGTCTGCTGACTCCAGTGCATCCATGAA
CTCTGGGGTTCTTCTGGTTCGGCCATCACGGCTCTCCTCCAGTGGGACTCCCATGCTAGCAGGGGTCTCTGAGTATGAGCTTCCCGAAGACCCTCGCTGG
GAGCTGCCTCGGGACAGACTGGTCTTAGGCAAACCCCTGGGAGAGGGCTGCTTTGGGCAGGTGGTGTTGGCAGAGGCTATCGGGCTGGACAAGGACAAAC
CCAACCGTGTGACCAAAGTGGCTGTGAAGATGTTGAAGTCGGACGCAACAGAGAAAGACTTGTCAGACCTGATCTCAGAAATGGAGATGATGAAGATGAT
CGGGAAGCATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTTGTATGTCATCGTGGAGTATGCCTCCAAGGGCAACCTGCGGGAG
TACCTGCAGGCCCGGAGGCCCCCAGGGCTGGAATACTGCTACAACCCCAGCCACAACCCAGAGGAGCAGCTCTCCTCCAAGGACCTGGTGTCCTGCGCCT
ACCAGGTGGCCCGAGGCATGGAGTATCTGGCCTCCAAGAAGTGCATACACCGAGACCTGGCAGCCAGGAATGTCCTGGTGACAGAGGACAATGTGATGAA
GATAGCAGACTTTGGCCTCGCACGGGACATTCACCACATCGACTACTATAAAAAGACAACCAACGGCCGACTGCCTGTGAAGTGGATGGCACCCGAGGCA
TTATTTGACCGGATCTACACCCACCAGAGTGATGTGTGGTCTTTCGGGGTGCTCCTGTGGGAGATCTTCACTCTGGGCGGCTCCCCATACCCCGGTGTGC
CTGTGGAGGAACTTTTCAAGCTGCTGAAGGAGGGTCACCGCATGGACAAGCCCAGTAACTGCACCAACGAGCTGTACATGATGATGCGGGACTGCTGGCA
TGCAGTGCCCTCACAGAGACCCACCTTCAAGCAGCTGGTGGAAGACCTGGACCGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATG
CCCCTGGACCAGTACTCCCCCAGCTTTCCCGACACCCGGAGCTCTACGTGCTCCTCAGGGGAGGATTCCGTCTTCTCTCATGAGCCGCTGCCCGAGGAGC
CCTGCCTGCCCCGACACCCAGCCCAGCTTGCCAATGGCGGACTCAAACGCCGCTGACTGCCACCCACACGCCCTCCCCAGACTCCACCGTCAGCTGTAAC
CCTCACCCACAGCCCCTGCTGGGCCCACCACCTGTCCGTCCCTGTCCCCTTTCCTGCTGGCAGGAGCCGGCTGCCTACCAGGGGCCTTCCTGTGTGGCCT
GCCTTCACCCCACTCAGCTCACCTCTCCCTCCACCTCCTCTCCACCTGCTGGTGAGAGGTGCAAAGAGGCAGATCTTTGCTGCCAGCCACTTCATCCCCT
CCCAGATGTTGGACCAACACCCCTCCCTGCCACCAGGCACTGCCTGGAGGGCAGGGAGTGGGAGCCAATGAACAGGCATGCAAGTGAGAGCTTCCTGAGC
TTTCTCCTGTCGGTTTGGTCTGTTTTGCCTTCACCCATAAGCCCCTCGCACTCTGGTGGCAGGTGCCTTGTCCTCAGGGCTACAGCAGTAGGGAGGTCAG
TGCTTCGTGCCTCGATTGAAGGTGACCTCTGCCCCAGATAGGTGGTGCCAGTGGCTTATTAATTCCGATACTAGTTTGCTTTGCTGACCAAATGCCTGGT
ACCAGAGGATGGTGAGGCGAAGGCCAGGTTGGGGGCAGTGTTGTGGCCCTGGGGCCCAGCCCCAAACTGGGGGCTCTGTATATAGCTATGAAGAAAACAC
AAAGTGTATAAATCTGAGTATATATTTACATGTCTTTTTAAAAGGGTCGTTACCAGAGATTTACCCATCGGGTAAGATGCTCCTGGTGGCTGGGAGGCAT
CAGTTGCTATATATTAAAAACAAAAAAGAAAAAAAAGGAAAATGTTTTTAAAAAGGTCATATATTTTTTGCTACTTTTGCTGTTTTATTTTTTTAAATTA
TGTTCTAAACCTATTTTCAGTTTAGGTCCCTCAATAAAAATTGCTGCTGCTTC
  • FGFR1.F11 (forward primer)
  • FGFR1.R11 (reverse primer)
  • Single exon skipping

Frame 0

PRRRIALGALLRPQARRPRRRAAASGSRDAGAA
AARGGTRVCRELGGHVRTGPRPLVAHCGDLAFPG
RERAAA*KAAEPKDFSPVRARGAPQGARYPCCS
RARRGAGASAGRWSPVCKESEAPPLRSGGGGHK
LRLHCSRPGWRRRAPSAAGSRAPAAELLRPRQDP
NRARGRRAGAGDAGTRPLAQATADSPEAEPPRR
ARVSLKRRIELTVEYPWRCGALSPTSNCRTGMW
SWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGA
PVEVESFLVHPGDLLQLRCRLRDDVQSINWLRD
GVQLAESNRTRITGEEVEVQDSVPADSGLYACV
TSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSS
EEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVP
AAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHR
IGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEY
GSINHTYQLDVVERSPHRPILQAGLPANKTVAL
GSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGP
DNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDA
GEYTCLAGNSIGLSHHSAWLTVLEALEERPAVM
TSPLYLEIIIYCTGAFLISCMVGSVIVYKMKSG
TKKSDFHSQMAVHKLAKSIPLRRQVSADSSASMN
SGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRW
ELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDK
PNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMI
GKHKNIINLLGACTQDGPLYVIVEYASKGNLRE
YLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCA
YQVARGMEYLASKKCIHRDLAARNVLVTEDNVMK
IADFGLARDIHHIDYYKKTTNGRLPVKWMAPEA
LFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGV
PVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWH
AVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSM
PLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEE
PCLPRHPAQLANGGLKRR*LPPTRPPQTPPSAVT
LTHSPCWAHHLSVPVPFPAGRSRLPTRGLPVWP
AFTPLSSPLPPPPLHLLVRGAKRQIFAASHFIP
SQMLDQHPSLPPGTAWRAGSGSQ*TGMQVRAS*A
FSCRFGLFCLHP*APRTLVAGALSSGLQQ*GGQ
CFVPRLKVTSAPDRWCQWLINSDTSLLC*PNAW
YQRMVRRRPGWGQCCGPGAQPQTGGSVYSYEENT
KCINLSIYLHVFLKGSLPEIYPSGKMLLVAGRH
QLLYIKNKKEKKGKCF*KGHIFFATFAVLFF*I
MF*TYFQFRSLNKNCCCF

Frame 1

PAGA*RSERSCGHRRGVLGGGRQLAGAGTPVQP
QRAEEPGCAGSWAATSGRDRDPS*RIAATSPSP
AASAPLLEKPRNPRTFLRSELGAPRRAHGTRAAV
GHAAAPGPPQGDGARSARKVRRRRCVLEEGGTS
SGSIVPARAGGAEHRAPPGVERRPRSSCDPART
RTEPGGGGPEPGTRAHARSHKPRRTLPRRNLHAE
RGSV*KGGSSSLWSIHGDVEPCHQPLTAELGCG
AGSASSSGLCWSQPHSAPLGRPRPCLNKPSPGE
PLWKWSPSWSTPVTCCSFAVGCGTMCRASTGCGT
GCSWRKATAPASQGRRWRCRTPCPQTPASMLA*
PAAPRAVTPPTSPSMFQMLSPPRRMMMMMMTPL
QRRKKQITPNQTVCP*LHIGHPQKRWKRNCMQCR
LPRQ*SSNALPVGPQTPHCAG*KMAKNSNLTTE
LEATRSVMPPGAS*WTLWCPLTRATTPALWRMS
TAASTTHTSWMSWSGPLTGPSCKQGCPPTKQWPW
VATWSSCVRCTVTRSRTSSG*STSR*MGARLAQ
TTCLMSRS*RLLELIPPTKRWRCFT*EMSPLRT
QGSIRAWRVTLSDSPITLHG*PFWKPWKRGRQ**
PRPCTWRSSSIAQGPSSSPAWWGRSSSTR*RVV
PRRVTSTARWLCTSWPRASLCADRCLLTPVHP*
TLGFFWFGHHGSPPVGLPC*QGSLSMSFPKTLAG
SCLGTDWS*ANPWERAALGRWCWQRLSGWTRTN
PTV*PKWL*RC*SRTQQRKTCQT*SQKWR**R*
SGSIRISSTCWGPARRMVPCMSSWSMPPRATCGS
TCRPGGPQGWNTATTPATTQRSSSPPRTWCPAP
TRWPEAWSIWPPRSAYTETWQPGMSW*QRTM**
R*QTLASHGTFTTSTTIKRQPTADCL*SGWHPRH
YLTGSTPTRVMCGLSGCSCGRSSLWAAPHTPVC
LWRNFSSC*RRVTAWTSPVTAPTSCT**CGTAG
MQCPHRDPPSSSWWKTWTASWP*PPTRSTWTCPC
PWTSTPPAFPTPGALRAPQGRIPSSLMSRCPRS
PACPDTQPSLPMADSNAADCHPHALPRLHRQL*
PSPTAPAGPTTCPSLSPFLLAGAGCLPGAFLCGL
PSPHSAHLSLHLLSTCW*EVQRGRSLLPATSSP
PRCWTNTPPCHQALPGGQGVGANEQACK*ELPE
LSPVGLVCFAFTHKPLALWWQVPCPQGYSSREVS
ASCLD*R*PLPQIGGASGLLIPILVCFADQMPG
TRGW*GEGQVGGSVVALGPSPKLGALYIAMKKT
QSV*I*VYIYMSF*KGRYQRFTHRVRCSWWLGGI
SCYILKTKKKKKENVFKKVIYFLLLLLFYFFKL
CSKPIFSLGPSIKIAAA

Frame 2

PPAHSARSALAATGAASSAAGGS*REPGRRCSR
SARRNPGVPGAGRPRPDGTETPRSALRRPRLPR
PRARRCLKSRGTQGLFSGPSSGRPAGRTVPVLQ
SGTPRRRGLRRAMEPGLQGK*GAAAAFWRRGAQA
PAPLFPPGLEAPSTERRRESSAGRGALATPPGP
EQSPGAAGRSRGRGHTPARTSHGGLSRGGTSTP
SEGQFEKEDRAHCGVSMEMWSLVTNL*LQNWDVE
LEVPPLLGCAGHSHTLHR*AVPDLA*TSPALGS
PCGSGVLPGPPR*PAAASLSAAGRCAEHQLAAG
RGAAGGKQPHPHHRGGGGGAGLRARRLRPLCLRN
QQPLGQ*HHLLLRQCFRCSPLLGG******LLF
RGERNR*HQTKPYARSSILDIPRKDGKEIACSA
GCQDSEVQMPFQWDPKPHTALVEKWQRIQT*PQN
WRLQGPLCHLEHHNGLCGAL*QGQLHLHCGE*V
RQHQPHIPAGCRGAVPSPAHPASRVARQQNSGP
G*QRGVHV*GVQ*PAAAHPVAKAHRGEWEQDWPR
QPALCPDLEDCWS*YHRQRDGGASLKKCLL*GR
RGVYVLGG*LYRTLPSLCMVDRSGSPGREAGSD
DLAPVPGDHHLLHRGLPHLLHGGVGHRLQDEEWY
QEE*LPQPDGCAQAGQEHPSAQTGVC*LQCIHE
LWGSSGSAITALLQWDSHASRGL*V*ASRRPSL
GAASGQTGLRQTPGRGLLWAGGVGRGYRAGQGQT
QPCDQSGCEDVEVGRNRERLVRPDLRNGDDEDD
REA*EYHQPAGGLHAGWSLVCHRGVCLQGQPAG
VPAGPEAPRAGILLQPQPQPRGAALLQGPGVLRL
PGGPRHGVSGLQEVHTPRPGSQECPGDRGQCDE
DSRLWPRTGHSPHRLL*KDNQRPTACEVDGTRG
II*PDLHPPE*CVVFRGAPVGDLHSGRLPIPRCA
CGGTFQAAEGGSPHGQAQ*LHQRAVHDDAGLLA
CSALTETHLQAAGGRPGPHRGLDLQPGVPGPVH
APGPVLPQLSRHPELYVLLRGGFRLLS*AAARGA
LPAPTPSPACQWRTQTPLTATHTPSPDSTVSCN
PHPQPLLGPPPVRPCPLSCWQEPAAYQGPSCVA
CLHPTQLTSPSTSSPPAGERCKEADLCCQPLHPL
PDVGPTPLPATRHCLEGREWEPMNRHASESFLS
FLLSVWSVLPSPISPSHSGGRCLVLRATAVGRS
VLRASIEGDLCPR*VVPVAY*FRY*FALLTKCLV
PEDGEAKARLGAVLWPWGPAPNWGLCI*L*RKH
KVYKSEYIFTCLFKRVVTRDLPIG*DAPGGWEA
SVAIY*KQKRKKRKMFLKRSYIFCYFCCFIFLNY
VLNLFSV*VPQ*KLLLL

Sequence FGFR1.bAug05

CCCCGCCGGCGCATAGCGCTCGGAGCGCTCTTGCGGCCACAGGCGCGGCGTCCTCGGCGGCGGGCGGCAGCTAGCGGGAGCCGGGACGCCGGTGCAGCCG
CAGCGCGCGGAGGAACCCGGGTGTGCCGGGAGCTGGGCGGCCACGTCCGGACGGGACCGAGACCCCTCGTAGCGCATTGCGGCGACCTCGCCTTCCCCGG
CCGCGAGCGCGCCGCTGCTTGAAAAGCCGCGGAACCCAAGGACTTTTCTCCGGTCCGAGCTCGGGGCGCCCCGCAGGGCGCACGGTACCCGTGCTGCAGT
CGGGCACGCCGCGGCGCCGGGGCCTCCGCAGGGCGATGGAGCCCGGTCTGCAAGGAAAGTGAGGCGCCGCCGCTGCGTTCTGGAGGAGGGGGGCACAAGG
TCTGGAGACCCCGGGTGGCGGACGGGAGCCCTCCCCCCGCCCCGCCTCCGGGGCACCAGCTCCGGCTCCATTGTTCCCGCCCGGGCTGGAGGCGCCGAGC
ACCGAGCGCCGCCGGGAGTCGAGCGCCGGCCGCGGAGCTCTTGCGACCCCGCCAGGACCCGAACAGAGCCCGGGGGCGGCGGGCCGGAGCCGGGGACGCG
GGCACACGCCCGCTCGCACAAGCCACGGCGGACTCTCCCGAGGCGGAACCTCCACGCCGAGCGAGGGTCAGTTTGAAAAGGAGGATCGAGCTCACTGTGG
AGTATCCATGGAGATGTGGAGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGAGCTGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGC
CACACTCTGCACCGCTAGGCCGTCCCCGACCTTGCCTGAACAAGCCCAGCCCTGGGGAGCCCCTGTGGAAGTGGAGTCCTTCCTGGTCCACCCCGGTGAC
CTGCTGCAGCTTCGCTGTCGGCTGCGGGACGATGTGCAGAGCATCAACTGGCTGCGGGACGGGGTGCAGCTGGCGGAAAGCAACCGCACCCGCATCACAG
GGGAGGAGGTGGAGGTGCAGGACTCCGTGCCCGCAGACTCCGGCCTCTATGCTTGCGTAACCAGCAGCCCCTCGGGCAGTGACACCACCTACTTCTCCGT
CAATGTTTCAGATGCTCTCCCCTCCTCGGAGGATGATGATGATGATGATGACTCCTCTTCAGAGGAGAAAGAAACAGATAACACCAAACCAAACCCCGTA
GCTCCATATTGGACATCCCCAGAAAAGATGGAAAAGAAATTGCATGCAGTGCCGGCTGCCAAGACAGTGAAGTTCAAATGCCCTTCCAGTGGGACCCCAA
ACCCCACACTGCGCTGGTTGAAAAATGGCAAAGAATTCAAACCTGACCACAGAATTGGAGGCTACAAGGTCCGTTATGCCACCTGGAGCATCATAATGGA
CTCTGTGGTGCCCTCTGACAAGGGCAACTACACCTGCATTGTGGAGAATGAGTACGGCAGCATCAACCACACATACCAGCTGGATGTCGTGGAGCGGTCC
CCTCACCGGCCCATCCTGCAAGCAGGGTTGCCCGCCAACAAAACAGTGGCCCTGGGTAGCAACGTGGAGTTCATGTGTAAGGTGTACAGTGACCCGCAGC
CGCACATCCAGTGGCTAAAGCACATCGAGGTGAATGGGAGCAAGATTGGCCCAGACAACCTGCCTTATGTCCAGATCTTGAAGACTGCTGGAGTTAATAC
CACCGACAAAGAGATGGAGGTGCTTCACTTAAGAAATGTCTCCTTTGAGGACGCAGGGGAGTATACGTGCTTGGCGGGTAACTCTATCGGACTCTCCCAT
CACTCTGCATGGTTGACCGTTCTGGAAGCCCTGGAAGAGAGGCCGGCAGTGATGACCTCGCCCCTGTACCTGGAGATCATCATCTATTGCACAGGGGCCT
TCCTCATCTCCTGCATGGTGGGGTCGGTCATCGTCTACAAGATGAAGAGTGGTACCAAGAAGAGTGACTTCCACAGCCAGATGGCTGTGCACAAGCTGGC
CAAGAGCATCCCTCTGCGCAGACAGGTAACAGTGTCTGCTGACTCCAGTGCATCCATGAACTCTGGGGTTCTTCTGGTTCGGCCATCACGGCTCTCCTCC
AGTGGGACTCCCATGCTAGCAGGGGTCTCTGAGTATGAGCTTCCCGAAGACCCTCGCTGGGAGCTGCCTCGGGACAGACTGGTCTTAGGCAAACCCCTGG
GAGAGGGCTGCTTTGGGCAGGTGGTGTTGGCAGAGGCTATCGGGCTGGACAAGGACAAACCCAACCGTGTGACCAAAGTGGCTGTGAAGATGTTGAAGTC
GGACGCAACAGAGAAAGACTTGTCAGACCTGATCTCAGAAATGGAGATGATGAAGATGATCGGGAAGCATAAGAATATCATCAACCTGCTGGGGGCCTGC
ACGCAGGATGGTCCCTTGTATGTCATCGTGGAGTATGCCTCCAAGGGCAACCTGCGGGAGTACCTGCAGGCCCGGAGGCCCCCAGGGCTGGAATACTGCT
ACAACCCCAGCCACAACCCAGAGGAGCAGCTCTCCTCCAAGGACCTGGTGTCCTGCGCCTACCAGGTGGCCCGAGGCATGGAGTATCTGGCCTCCAAGAA
GTGCATACACCGAGACCTGGCAGCCAGGAATGTCCTGGTGACAGAGGACAATGTGATGAAGATAGCAGACTTTGGCCTCGCACGGGACATTCACCACATC
GACTACTATAAAAAGACAACCAACGGCCGACTGCCTGTGAAGTGGATGGCACCCGAGGCATTATTTGACCGGATCTACACCCACCAGAGTGATGTGTGGT
CTTTCGGGGTGCTCCTGTGGGAGATCTTCACTCTGGGCGGCTCCCCATACCCCGGTGTGCCTGTGGAGGAACTTTTCAAGCTGCTGAAGGAGGGTCACCG
CATGGACAAGCCCAGTAACTGCACCAACGAGCTGTACATGATGATGCGGGACTGCTGGCATGCAGTGCCCTCACAGAGACCCACCTTCAAGCAGCTGGTG
GAAGACCTGGACCGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCCCCTGGACCAGTACTCCCCCAGCTTTCCCGACACCCGGA
GCTCTACGTGCTCCTCAGGGGAGGATTCCGTCTTCTCTCATGAGCCGCTGCCCGAGGAGCCCTGCCTGCCCCGACACCCAGCCCAGCTTGCCAATGGCGG
ACTCAAACGCCGCTGACTGCCACCCACACGCCCTCCCCAGACTCCACCGTCAGCTGTAACCCTCACCCACAGCCCCTGCTGGGCCCACCACCTGTCCGTC
CCTGTCCCCTTTCCTGCTGGCAGGAGCCGGCTGCCTACCAGGGGCCTTCCTGTGTGGCCTGCCTTCACCCCACTCAGCTCACCTCTCCCTCCACCTCCTC
TCCACCTGCTGGTGAGAGGTGCAAAGAGGCAGATCTTTGCTGCCAGCCACTTCATCCCCTCCCAGATGTTGGACCAACACCCCTCCCTGCCACCAGGCAC
TGCCTGGAGGGCAGGGAGTGGGAGCCAATGAACAGGCATGCAAGTGAGAGCTTCCTGAGCTTTCTCCTGTCGGTTTGGTCTGTTTTGCCTTCACCCATAA
GCCCCTCGCACTCTGGTGGCAGGTGCCTTGTCCTCAGGGCTACAGCAGTAGGGAGGTCAGTGCTTCGTGCCTCGATTGAAGGTGACCTCTGCCCCAGATA
GGTGGTGCCAGTGGCTTATTAATTCCGATACTAGTTTGCTTTGCTGACCAAATGCCTGGTACCAGAGGATGGTGAGGCGAAGGCCAGGTTGGGGGCAGTG
TTGTGGCCCTGGGGCCCAGCCCCAAACTGGGGGCTCTGTATATAGCTATGAAGAAAACACAAAGTGTATAAATCTGAGTATATATTTACATGTCTTTTTA
AAAGGGTCGTTACCAGAGATTTACCCATCGGGTAAGATGCTCCTGGTGGCTGGGAGGCATCAGTTGCTATATATTAAAAACAAAAAAGAAAAAAAAGGAA
AATGTTTTTAAAAAGGTCATATATTTTTTGCTACTTTTGCTGTTTTATTTTTTTAAATTATGTTCTAAACCTATTTTCAGTTTAGGTCCCTCAATAAAAA
TTGCTGCTGCTTCATTTATCTATGGGCTGTATGAAAAGGGTGGGAATGTCCACTGGAAAGAAGGGACACCCACGGGCCCTGGGGCTAGGTCTGTCCCGAG
GGCACCGCATGCTCCCGGCGCAGGTTCCTTGTAACCTCTTCTTCCTAGGTCCTGCACCCAGACCTCACGACGCACCTCCTGCCTCTCCGCTGCTTTTGGA
AAGTCAGAAAAAGAAGATGTCTGCTTCGAGGGCAGGAACCCCATCCATGCAGTAGAGGCGCTGGGCAGAGAGTCAAGGCCCAGCAGCCATCGACCATGGA
TGGTTTCCTCCAAGGAAACCGGTGGGGTTGGGCTGGGGAGGGGGCACCTACCTAGGAATAGCCACGGGGTAGAGCTACAGTGATTAAGAGGAAAGCAAGG
GCGCGGTTGCTCACGCCTGTAATCCCAGCACTTTGGGACACCGAGGTGGGCAGATCACTTCAGGTCAGGAGTTTGAGACCAGCCTGGCCAACTTAGTGAA
ACCCCATCTCTACTAAAAATGCAAAAATTATCCAGGCATGGTGGCACACGCCTGTAATCCCAGCTCCACAGGAGGCTGAGGCAGAATCCCTTGAAGCTGG
GAGGCGGAGGTTGCAGTGAGCCGAGATTGCGCCATTGCACTCCAGCCTGGGCAACAGAGAAAACAAAAAGGAAAACAAATGATGAAGGTCTGCAGAAACT
GAAACCCAGACATGTGTCTGCCCCCTCTATGTGGGCATGGTTTTGCCAGTGCTTCTAAGTGCAGGAGAACATGTCACCTGAGGCTAGTTTTGCATTCAGG
TCCCTGGCTTCGTTTCTTGTTGGTATGCCTCCCCAGATCGTCCTTCCTGTATCCATGTGACCAGACTGTATTTGTTGGGACTGTCGCAGATCTTGGCTTC
TTACAGTTCTTCCTGTCCAAACTCCATCCTGTCCCTCAGGAACGGGGGGAAAATTCTCCGAATGTTTTTGGTTTTTTGGCTGCTTGGAATTTACTTCTGC
CACCTGCTGGTCATCACTGTCCTCACTAAGTGGATTCTGGCTCCCCCGTACCTCATGGCTCAAACTACCACTCCTCAGTCGCTATATTAAAGCTTATATT
TTGCTGGATTACTGCTAAATACAAAAGAAAGTTCAATATGTTTTCATTTCTGTAGGGAAAATGGGATTGCTGCTTTAAATTTCTGAGCTAGGGATTTTTT
GGCAGCTGCAGTGTTGGCGACTATTGTAAAATTCTCTTTGTTTCTCTCTGTAAATAGCACCTGCTAACATTACAATTTGTATTTATGTTTAAAGAAGGCA
TCATTTGGTGAACAGAACTAGGAAATGAATTGTATTTTTAGTAGAGACGGTGTTTCACCATGTTGGCCAAGCTGGTCTTGAACTCCTGACCTCGGGTGAT
CCACCCACCTCAGCCTCCCAAAGTGCTGGATTACAGGCATGAGCCACTGCTACTGGCCTCAGTTATTTTCTAATAG
  • FGFR1.F11 (forward primer)
  • FGFR1.R11 (reverse primer)
  • Single exon skipping

Frame 0

PRRRIALGALLRPQARRPRRRAAASGSRDAGAA
AARGGTRVCRELGGHVRTGPRPLVAHCGDLAFPG
RERAAA*KAAEPKDFSPVRARGAPQGARYPCCS
RARRGAGASAGRWSPVCKESEAPPLRSGGGGHK
VWRPRVADGSPPPAPPPGHQLRLHCSRPGWRRRA
PSAAGSRAPAAELLRPRQDPNRARGRRAGAGDA
GTRPLAQATADSPEAEPPRRARVSLKRRIELTV
EYPWRCGALSPTSNCRTGMWSWKCLLFWAVLVTA
TLCTARPSPTLPEQAQPWGAPVEVESFLVHPGD
LLQLRCRLRDDVQSINWLRDGVQLAESNRTRIT
GEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSV
NVSDALPSSEDDDDDDDSSSEEKETDNTKPNPV
APYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTP
NPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMD
SVVPSDKGNYTCIVENEYGSINHTYQLDVVERS
PHRPILQAGLPANKTVALGSNVEFMCKVYSDPQ
PHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNT
TDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSH
HSAWLTVLEALEERPAVMTSPLYLEIIIYCTGA
FLISCMVGSVIVYKMKSGTKKSDFHSQMAVHKLA
KSIPLRRQVTVSADSSASMNSGVLLVRPSRLSS
SGTPMLAGVSEYELPEDPRWELPRDRLVLGKPL
GEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKS
DATEKDLSDLISEMEMMKMIGKHKNIINLLGAC
TQDGPLYVIVEYASKGNLREYLQARRPPGLEYC
YNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKK
CIHRDLAARNVLVTEDNVMKIADFGLARDIHHI
DYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVW
SFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHR
MDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLV
EDLDRIVALTSNQEYLDLSMPLDQYSPSFPDTR
SSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGG
LKRR*LPPTRPPQTPPSAVTLTHSPCWAHHLSV
PVPFPAGRSRLPTRGLPVWPAFTPLSSPLPPPP
LHLLVRGAKRQIFAASHFIPSQMLDQHPSLPPGT
AWRAGSGSQ*TGMQVRAS*AFSCRFGLFCLHP*
APRTLVAGALSSGLQQ*GGQCFVPRLKVTSAPD
RWCQWLINSDTSLLC*PNAWYQRMVRRRPGWGQC
CGPGAQPQTGGSVYSYEENTKCINLSIYLHVFL
KGSLPEIYPSGKMLLVAGRHQLLYIKNKKEKKG
KCF*KGHIFFATFAVLFF*IMF*TYFQFRSLNKN
CCCFIYLWAV*KGWECPLERRDTHGPWG*VCPE
GTACSRRRFLVTSSS*VLHPDLTTHLLPLRCFW
KVRKRRCLLRGQEPHPCSRGAGQRVKAQQPSTMD
GFLQGNRWGWAGEGAPT*E*PRGRATVIKRKAR
ARLLTPVIPALWDTEVGRSLQVRSLRPAWPT**
NPISTKNAKIIQAWWHTPVIPAPQEAEAESLEAG
RRRLQ*AEIAPLHSSLGNRENKKENK**RSAET
ETQTCVCPLYVGMVLPVLLSAGEHVT*G*FCIQ
VPGFVSCWYASPDRPSCIHVTRLYLLGLSQILAS
YSSSCPNSILSLRNGGKILRMFLVFWLLGIYFC
HLLVITVLTKWILAPPYLMAQTTTPQSLY*SLY
FAGLLLNTKESSICFHFCRENGIAALNF*ARDFL
AAAVLATIVKFSLFLSVNSTC*HYNLYLCLKKA
SFGEQN*EMNCIFSRDGVSPCWPSWS*TPDLG*
STHLSLPKCWITGMSHCYWPQLFSN

Frame 1

PAGA*RSERSCGHRRGVLGGGRQLAGAGTPVQP
QRAEEPGCAGSWAATSGRDRDPS*RIAATSPSP
AASAPLLEKPRNPRTFLRSELGAPRRAHGTRAAV
GHAAAPGPPQGDGARSARKVRRRRCVLEEGGTR
SGDPGWRTGALPPPRLRGTSSGSIVPARAGGAE
HRAPPGVERRPRSSCDPARTRTEPGGGGPEPGTR
AHARSHKPRRTLPRRNLHAERGSV*KGGSSSLW
SIHGDVEPCHQPLTAELGCGAGSASSSGLCWSQ
PHSAPLGRPRPCLNKPSPGEPLWKWSPSWSTPVT
CCSFAVGCGTMCRASTGCGTGCSWRKATAPASQ
GRRWRCRTPCPQTPASMLA*PAAPRAVTPPTSP
SMFQMLSPPRRMMMMMMTPLQRRKKQITPNQTP*
LHIGHPQKRWKRNCMQCRLPRQ*SSNALPVGPQ
TPHCAG*KMAKNSNLTTELEATRSVMPPGAS*W
TLWCPLTRATTPALWRMSTAASTTHTSWMSWSGP
LTGPSCKQGCPPTKQWPWVATWSSCVRCTVTRS
RTSSG*STSR*MGARLAQTTCLMSRS*RLLELI
PPTKRWRCFT*EMSPLRTQGSIRAWRVTLSDSPI
TLHG*PFWKPWKRGRQ**PRPCTWRSSSIAQGP
SSSPAWWGRSSSTR*RVVPRRVTSTARWLCTSW
PRASLCADR*QCLLTPVHP*TLGFFWFGHHGSPP
VGLPC*QGSLSMSFPKTLAGSCLGTDWS*ANPW
ERAALGRWCWQRLSGWTRTNPTV*PKWL*RC*S
RTQQRKTCQT*SQKWR**R*SGSIRISSTCWGPA
RRMVPCMSSWSMPPRATCGSTCRPGGPQGWNTA
TTPATTQRSSSPPRTWCPAPTRWPEAWSIWPPR
SAYTETWQPGMSW*QRTM**R*QTLASHGTFTTS
TTIKRQPTADCL*SGWHPRHYLTGSTPTRVMCG
LSGCSCGRSSLWAAPHTPVCLWRNFSSC*RRVT
AWTSPVTAPTSCT**CGTAGMQCPHRDPPSSSWW
KTWTASWP*PPTRSTWTCPCPWTSTPPAFPTPG
ALRAPQGRIPSSLMSRCPRSPACPDTQPSLPMA
DSNAADCHPHALPRLHRQL*PSPTAPAGPTTCPS
LSPFLLAGAGCLPGAFLCGLPSPHSAHLSLHLL
STCW*EVQRGRSLLPATSSPPRCWTNTPPCHQA
LPGGQGVGANEQACK*ELPELSPVGLVCFAFTHK
PLALWWQVPCPQGYSSREVSASCLD*R*PLPQI
GGASGLLIPILVCFADQMPGTRGW*GEGQVGGS
VVALGPSPKLGALYIAMKKTQSV*I*VYIYMSF*
KGRYQRFTHRVRCSWWLGGISCYILKTKKKKKE
NVFKKVIYFLLLLLFYFFKLCSKPIFSLGPSIK
IAAASFIYGLYEKGGNVHWKEGTPTGPGARSVPR
APHAPGAGSL*PLLPRSCTQTSRRTSCLSAAFG
KSEKEDVCFEGRNPIHAVEALGRESRPSSHRPW
MVSSKETGGVGLGRGHLPRNSHGVELQ*LRGKQG
RGCSRL*SQHFGTPRWADHFRSGV*DQPGQLSE
TPSLLKMQKLSRHGGTRL*SQLHRRLRQNPLKL
GGGGCSEPRLRHCTPAWATEKTKRKTNDEGLQKL
KPRHVSAPSMWAWFCQCF*VQENMSPEASFAFR
SLASFLVGMPPQIVLPVSM*PDCICWDCRRSWL
LTVLPVQTPSCPSGTGGKFSECFWFFGCLEFTSA
TCWSSLSSLSGFWLPRTSWLKLPLLSRYIKAYI
LLDYC*IQKKVQYVFISVGKMGLLL*ISELGIF
WQLQCWRLL*NSLCFSL*IAPANITICIYV*RRH
HLVNRTRK*IVFLVETVFHHVGQAGLELLTSGD
PPTSASQSAGLQA*ATATGLSYFLI

Frame 2

PPAHSARSALAATGAASSAAGGS*REPGRRCSR
SARRNPGVPGAGRPRPDGTETPRSALRRPRLPR
PRARRCLKSRGTQGLFSGPSSGRPAGRTVPVLQ
SGTPRRRGLRRAMEPGLQGK*GAAAAFWRRGAQG
LETPGGGREPSPRPASGAPAPAPLFPPGLEAPS
TERRRESSAGRGALATPPGPEQSPGAAGRSRGR
GHTPARTSHGGLSRGGTSTPSEGQFEKEDRAHCG
VSMEMWSLVTNL*LQNWDVELEVPPLLGCAGHS
HTLHR*AVPDLA*TSPALGSPCGSGVLPGPPR*
PAAASLSAAGRCAEHQLAAGRGAAGGKQPHPHHR
GGGGGAGLRARRLRPLCLRNQQPLGQ*HHLLLR
QCFRCSPLLGG******LLFRGERNR*HQTKPR
SSILDIPRKDGKEIACSAGCQDSEVQMPFQWDPK
PHTALVEKWQRIQT*PQNWRLQGPLCHLEHHNG
LCGAL*QGQLHLHCGE*VRQHQPHIPAGCRGAV
PSPAHPASRVARQQNSGPG*QRGVHV*GVQ*PAA
AHPVAKAHRGEWEQDWPRQPALCPDLEDCWS*Y
HRQRDGGASLKKCLL*GRRGVYVLGG*LYRTLP
SLCMVDRSGSPGREAGSDDLAPVPGDHHLLHRGL
PHLLHGGVGHRLQDEEWYQEE*LPQPDGCAQAG
QEHPSAQTGNSVC*LQCIHELWGSSGSAITALL
QWDSHASRGL*V*ASRRPSLGAASGQTGLRQTPG
RGLLWAGGVGRGYRAGQGQTQPCDQSGCEDVEV
GRNRERLVRPDLRNGDDEDDREA*EYHQPAGGL
HAGWSLVCHRGVCLQGQPAGVPAGPEAPRAGILL
QPQPQPRGAALLQGPGVLRLPGGPRHGVSGLQE
VHTPRPGSQECPGDRGQCDEDSRLWPRTGHSPH
RLL*KDNQRPTACEVDGTRGII*PDLHPPE*CVV
FRGAPVGDLHSGRLPIPRCACGGTFQAAEGGSP
HGQAQ*LHQRAVHDDAGLLACSALTETHLQAAG
GRPGPHRGLDLQPGVPGPVHAPGPVLPQLSRHPE
LYVLLRGGFRLLS*AAARGALPAPTPSPACQWR
TQTPLTATHTPSPDSTVSCNPHPQPLLGPPPVR
PCPLSCWQEPAAYQGPSCVACLHPTQLTSPSTSS
PPAGERCKEADLCCQPLHPLPDVGPTPLPATRH
CLEGREWEPMNRHASESFLSFLLSVWSVLPSPI
SPSHSGGRCLVLRATAVGRSVLRASIEGDLCPR*
VVPVAY*FRY*FALLTKCLVPEDGEAKARLGAV
LWPWGPAPNWGLCI*L*RKHKVYKSEYIFTCLF
KRVVTRDLPIG*DAPGGWEASVAIY*KQKRKKRK
MFLKRSYIFCYFCCFIFLNYVLNLFSV*VPQ*K
LLLLHLSMGCMKRVGMSTGKKGHPRALGLGLSR
GHRMLPAQVPCNLFFLGPAPRPHDAPPASPLLLE
SQKKKMSASRAGTPSMQ*RRWAESQGPAAIDHG
WFPPRKPVGLGWGGGTYLGIATG*SYSD*EESK
GAVAHACNPSTLGHRGGQITSGQEFETSLANLVK
PHLY*KCKNYPGMVAHACNPSSTGG*GRIP*SW
EAEVAVSRDCAIALQPGQQRKQKGKQMMKVCRN
*NPDMCLPPLCGHGFASASKCRRTCHLRLVLHSG
PWLRFLLVCLPRSSFLYPCDQTVFVGTVADLGF
LQFFLSKLHPVPQERGENSPNVFGFLAAWNLLL
PPAGHHCPH*VDSGSPVPHGSNYHSSVAILKLIF
CWITAKYKRKFNMFSFL*GKWDCCFKFLS*GFF
GSCSVGDYCKILFVSLCK*HLLTLQFVFMFKEG
IIW*TELGNELYF**RRCFTMLAKLVLNS*PRVI
HPPQPPKVLDYRHEPLLLASVIF**

Sequence FGFR1.aAug05

CCCCGCCGGCGCATAGCGCTCGGAGCGCTCTTGCGGCCACAGGCGCGGCGTCCTCGGCGGCGGGCGGCAGCTAGCGGGAGCCGGGACGCCGGTGCAGCCG
CAGCGCGCGGAGGAACCCGGGTGTGCCGGGAGCTGGGCGGCCACGTCCGGACGGGACCGAGACCCCTCGTAGCGCATTGCGGCGACCTCGCCTTCCCCGG
CCGCGAGCGCGCCGCTGCTTGAAAAGCCGCGGAACCCAAGGACTTTTCTCCGGTCCGAGCTCGGGGCGCCCCGCAGGGCGCACGGTACCCGTGCTGCAGT
CGGGCACGCCGCGGCGCCGGGGCCTCCGCAGGGCGATGGAGCCCGGTCTGCAAGGAAAGTGAGGCGCCGCCGCTGCGTTCTGGAGGAGGGGGGCACAAGG
TCTGGAGACCCCGGGTGGCGGACGGGAGCCCTCCCCCCGCCCCGCCTCCGGGGCACCAGCTCCGGCTCCATTGTTCCCGCCCGGGCTGGAGGCGCCGAGC
ACCGAGCGCCGCCGGGAGTCGAGCGCCGGCCGCGGAGCTCTTGCGACCCCGCCAGGACCCGAACAGAGCCCGGGGGCGGCGGGCCGGAGCCGGGGACGCG
GGCACACGCCCGCTCGCACAAGCCACGGCGGACTCTCCCGAGGCGGAACCTCCACGCCGAGCGAGGGTCAGTTTGAAAAGGAGGATCGAGCTCACTGTGG
AGTATCCATGGAGATGTGGAGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGAGCTGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGC
CACACTCTGCACCGCTAGGCCGTCCCCGACCTTGCCTGAACAAGCCCAGCCCTGGGGAGCCCCTGTGGAAGTGGAGTCCTTCCTGGTCCACCCCGGTGAC
CTGCTGCAGCTTCGCTGTCGGCTGCGGGACGATGTGCAGAGCATCAACTGGCTGCGGGACGGGGTGCAGCTGGCGGAAAGCAACCGCACCCGCATCACAG
GGGAGGAGGTGGAGGTGCAGGACTCCGTGCCCGCAGACTCCGGCCTCTATGCTTGCGTAACCAGCAGCCCCTCGGGCAGTGACACCACCTACTTCTCCGT
CAATGTTTCAGATGCTCTCCCCTCCTCGGAGGATGATGATGATGATGATGACTCCTCTTCAGAGGAGAAAGAAACAGATAACACCAAACCAAACCGTATG
CCCGTAGCTCCATATTGGACATCCCCAGAAAAGATGGAAAAGAAATTGCATGCAGTGCCGGCTGCCAAGACAGTGAAGTTCAAATGCCCTTCCAGTGGGA
CCCCAAACCCCACACTGCGCTGGTTGAAAAATGGCAAAGAATTCAAACCTGACCACAGAATTGGAGGCTACAAGGTCCGTTATGCCACCTGGAGCATCAT
AATGGACTCTGTGGTGCCCTCTGACAAGGGCAACTACACCTGCATTGTGGAGAATGAGTACGGCAGCATCAACCACACATACCAGCTGGATGTCGTGGAG
CGGTCCCCTCACCGGCCCATCCTGCAAGCAGGGTTGCCCGCCAACAAAACAGTGGCCCTGGGTAGCAACGTGGAGTTCATGTGTAAGGTGTACAGTGACC
CGCAGCCGCACATCCAGTGGCTAAAGCACATCGAGGTGAATGGGAGCAAGATTGGCCCAGACAACCTGCCTTATGTCCAGATCTTGAAGACTGCTGGAGT
TAATACCACCGACAAAGAGATGGAGGTGCTTCACTTAAGAAATGTCTCCTTTGAGGACGCAGGGGAGTATACGTGCTTGGCGGGTAACTCTATCGGACTC
TCCCATCACTCTGCATGGTTGACCGTTCTGGAAGCCCTGGAAGAGAGGCCGGCAGTGATGACCTCGCCCCTGTACCTGGAGATCATCATCTATTGCACAG
GGGCCTTCCTCATCTCCTGCATGGTGGGGTCGGTCATCGTCTACAAGATGAAGAGTGGTACCAAGAAGAGTGACTTCCACAGCCAGATGGCTGTGCACAA
GCTGGCCAAGAGCATCCCTCTGCGCAGACAGGTAACAGTGTCTGCTGACTCCAGTGCATCCATGAACTCTGGGGTTCTTCTGGTTCGGCCATCACGGCTC
TCCTCCAGTGGGACTCCCATGCTAGCAGGGGTCTCTGAGTATGAGCTTCCCGAAGACCCTCGCTGGGAGCTGCCTCGGGACAGACTGGTCTTAGGCAAAC
CCCTGGGAGAGGGCTGCTTTGGGCAGGTGGTGTTGGCAGAGGCTATCGGGCTGGACAAGGACAAACCCAACCGTGTGACCAAAGTGGCTGTGAAGATGTT
GAAGTCGGACGCAACAGAGAAAGACTTGTCAGACCTGATCTCAGAAATGGAGATGATGAAGATGATCGGGAAGCATAAGAATATCATCAACCTGCTGGGG
GCCTGCACGCAGGATGGTCCCTTGTATGTCATCGTGGAGTATGCCTCCAAGGGCAACCTGCGGGAGTACCTGCAGGCCCGGAGGCCCCCAGGGCTGGAAT
ACTGCTACAACCCCAGCCACAACCCAGAGGAGCAGCTCTCCTCCAAGGACCTGGTGTCCTGCGCCTACCAGGTGGCCCGAGGCATGGAGTATCTGGCCTC
CAAGAAGTGCATACACCGAGACCTGGCAGCCAGGAATGTCCTGGTGACAGAGGACAATGTGATGAAGATAGCAGACTTTGGCCTCGCACGGGACATTCAC
CACATCGACTACTATAAAAAGACAACCAACGGCCGACTGCCTGTGAAGTGGATGGCACCCGAGGCATTATTTGACCGGATCTACACCCACCAGAGTGATG
TGTGGTCTTTCGGGGTGCTCCTGTGGGAGATCTTCACTCTGGGCGGCTCCCCATACCCCGGTGTGCCTGTGGAGGAACTTTTCAAGCTGCTGAAGGAGGG
TCACCGCATGGACAAGCCCAGTAACTGCACCAACGAGCTGTACATGATGATGCGGGACTGCTGGCATGCAGTGCCCTCACAGAGACCCACCTTCAAGCAG
CTGGTGGAAGACCTGGACCGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCCCCTGGACCAGTACTCCCCCAGCTTTCCCGACA
CCCGGAGCTCTACGTGCTCCTCAGGGGAGGATTCCGTCTTCTCTCATGAGCCGCTGCCCGAGGAGCCCTGCCTGCCCCGACACCCAGCCCAGCTTGCCAA
TGGCGGACTCAAACGCCGCTGACTGCCACCCACACGCCCTCCCCAGACTCCACCGTCAGCTGTAACCCTCACCCACAGCCCCTGCTGGGCCCACCACCTG
TCCGTCCCTGTCCCCTTTCCTGCTGGCAGGAGCCGGCTGCCTACCAGGGGCCTTCCTGTGTGGCCTGCCTTCACCCCACTCAGCTCACCTCTCCCTCCAC
CTCCTCTCCACCTGCTGGTGAGAGGTGCAAAGAGGCAGATCTTTGCTGCCAGCCACTTCATCCCCTCCCAGATGTTGGACCAACACCCCTCCCTGCCACC
AGGCACTGCCTGGAGGGCAGGGAGTGGGAGCCAATGAACAGGCATGCAAGTGAGAGCTTCCTGAGCTTTCTCCTGTCGGTTTGGTCTGTTTTGCCTTCAC
CCATAAGCCCCTCGCACTCTGGTGGCAGGTGCCTTGTCCTCAGGGCTACAGCAGTAGGGAGGTCAGTGCTTCGTGCCTCGATTGAAGGTGACCTCTGCCC
CAGATAGGTGGTGCCAGTGGCTTATTAATTCCGATACTAGTTTGCTTTGCTGACCAAATGCCTGGTACCAGAGGATGGTGAGGCGAAGGCCAGGTTGGGG
GCAGTGTTGTGGCCCTGGGGCCCAGCCCCAAACTGGGGGCTCTGTATATAGCTATGAAGAAAACACAAAGTGTATAAATCTGAGTATATATTTACATGTC
TTTTTAAAAGGGTCGTTACCAGAGATTTACCCATCGGGTAAGATGCTCCTGGTGGCTGGGAGGCATCAGTTGCTATATATTAAAAACAAAAAAGAAAAAA
AAGGAAAATGTTTTTAAAAAGGTCATATATTTTTTGCTACTTTTGCTGTTTTATTTTTTTAAATTATGTTCTAAACCTATTTTCAGTTTAGGTCCCTCAA
TAAAAATTGCTGCTGCTTC
  • FGFR1.F11 (forward primer)
  • FGFR1.R11 (reverse primer)
  • Single exon skipping

Frame 0

PRRRIALGALLRPQARRPRRRAAASGSRDAGAA
AARGGTRVCRELGGHVRTGPRPLVAHCGDLAFPG
RERAAA*KAAEPKDFSPVRARGAPQGARYPCCS
RARRGAGASAGRWSPVCKESEAPPLRSGGGGHK
VWRPRVADGSPPPAPPPGHQLRLHCSRPGWRRRA
PSAAGSRAPAAELLRPRQDPNRARGRRAGAGDA
GTRPLAQATADSPEAEPPRRARVSLKRRIELTV
EYPWRCGALSPTSNCRTGMWSWKCLLFWAVLVTA
TLCTARPSPTLPEQAQPWGAPVEVESFLVHPGD
LLQLRCRLRDDVQSINWLRDGVQLAESNRTRIT
GEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSV
NVSDALPSSEDDDDDDDSSSEEKETDNTKPNRM
PVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSG
TPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSII
MDSVVPSDKGNYTCIVENEYGSINHTYQLDVVE
RSPHRPILQAGLPANKTVALGSNVEFMCKVYSD
PQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGV
NTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGL
SHHSAWLTVLEALEERPAVMTSPLYLEIIIYCT
GAFLISCMVGSVIVYKMKSGTKKSDFHSQMAVHK
LAKSIPLRRQVTVSADSSASMNSGVLLVRPSRL
SSSGTPMLAGVSEYELPEDPRWELPRDRLVLGK
PLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKML
KSDATEKDLSDLISEMEMMKMIGKHKNIINLLG
ACTQDGPLYVIVEYASKGNLREYLQARRPPGLE
YCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLAS
KKCIHRDLAARNVLVTEDNVMKIADFGLARDIH
HIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSD
VWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEG
HRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQ
LVEDLDRIVALTSNQEYLDLSMPLDQYSPSFPD
TRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLAN
GGLKRR*LPPTRPPQTPPSAVTLTHSPCWAHHL
SVPVPFPAGRSRLPTRGLPVWPAFTPLSSPLPP
PPLHLLVRGAKRQIFAASHFIPSQMLDQHPSLPP
GTAWRAGSGSQ*TGMQVRAS*AFSCRFGLFCLH
P*APRTLVAGALSSGLQQ*GGQCFVPRLKVTSA
PDRWCQWLINSDTSLLC*PNAWYQRMVRRRPGWG
QCCGPGAQPQTGGSVYSYEENTKCINLSIYLHV
FLKGSLPEIYPSGKMLLVAGRHQLLYIKNKKEK
KGKCF*KGHIFFATFAVLFF*IMF*TYFQFRSLN
KNCCCF

Frame 1

PAGA*RSERSCGHRRGVLGGGRQLAGAGTPVQP
QRAEEPGCAGSWAATSGRDRDPS*RIAATSPSP
AASAPLLEKPRNPRTFLRSELGAPRRAHGTRAAV
GHAAAPGPPQGDGARSARKVRRRRCVLEEGGTR
SGDPGWRTGALPPPRLRGTSSGSIVPARAGGAE
HRAPPGVERRPRSSCDPARTRTEPGGGGPEPGTR
AHARSHKPRRTLPRRNLHAERGSV*KGGSSSLW
SIHGDVEPCHQPLTAELGCGAGSASSSGLCWSQ
PHSAPLGRPRPCLNKPSPGEPLWKWSPSWSTPVT
CCSFAVGCGTMCRASTGCGTGCSWRKATAPASQ
GRRWRCRTPCPQTPASMLA*PAAPRAVTPPTSP
SMFQMLSPPRRMMMMMMTPLQRRKKQITPNQTVC
P*LHIGHPQKRWKRNCMQCRLPRQ*SSNALPVG
PQTPHCAG*KMAKNSNLTTELEATRSVMPPGAS
*WTLWCPLTRATTPALWRMSTAASTTHTSWMSWS
GPLTGPSCKQGCPPTKQWPWVATWSSCVRCTVT
RSRTSSG*STSR*MGARLAQTTCLMSRS*RLLE
LIPPTKRWRCFT*EMSPLRTQGSIRAWRVTLSDS
PITLHG*PFWKPWKRGRQ**PRPCTWRSSSIAQ
GPSSSPAWWGRSSSTR*RVVPRRVTSTARWLCT
SWPRASLCADR*QCLLTPVHP*TLGFFWFGHHGS
PPVGLPC*QGSLSMSFPKTLAGSCLGTDWS*AN
PWERAALGRWCWQRLSGWTRTNPTV*PKWL*RC
*SRTQQRKTCQT*SQKWR**R*SGSIRISSTCWG
PARRMVPCMSSWSMPPRATCGSTCRPGGPQGWN
TATTPATTQRSSSPPRTWCPAPTRWPEAWSIWP
PRSAYTETWQPGMSW*QRTM**R*QTLASHGTFT
TSTTIKRQPTADCL*SGWHPRHYLTGSTPTRVM
CGLSGCSCGRSSLWAAPHTPVCLWRNFSSC*RR
VTAWTSPVTAPTSCT**CGTAGMQCPHRDPPSSS
WWKTWTASWP*PPTRSTWTCPCPWTSTPPAFPT
PGALRAPQGRIPSSLMSRCPRSPACPDTQPSLP
MADSNAADCHPHALPRLHRQL*PSPTAPAGPTTC
PSLSPFLLAGAGCLPGAFLCGLPSPHSAHLSLH
LLSTCW*EVQRGRSLLPATSSPPRCWTNTPPCH
QALPGGQGVGANEQACK*ELPELSPVGLVCFAFT
HKPLALWWQVPCPQGYSSREVSASCLD*R*PLP
QIGGASGLLIPILVCFADQMPGTRGW*GEGQVG
GSVVALGPSPKLGALYIAMKKTQSV*I*VYIYMS
F*KGRYQRFTHRVRCSWWLGGISCYILKTKKKK
KENVFKKVIYFLLLLLFYFFKLCSKPIFSLGPS
IKIAAA

Frame 2

PPAHSARSALAATGAASSAAGGS*REPGRRCSR
SARRNPGVPGAGRPRPDGTETPRSALRRPRLPR
PRARRCLKSRGTQGLFSGPSSGRPAGRTVPVLQ
SGTPRRRGLRRAMEPGLQGK*GAAAAFWRRGAQG
LETPGGGREPSPRPASGAPAPAPLFPPGLEAPS
TERRRESSAGRGALATPPGPEQSPGAAGRSRGR
GHTPARTSHGGLSRGGTSTPSEGQFEKEDRAHCG
VSMEMWSLVTNL*LQNWDVELEVPPLLGCAGHS
HTLHR*AVPDLA*TSPALGSPCGSGVLPGPPR*
PAAASLSAAGRCAEHQLAAGRGAAGGKQPHPHHR
GGGGGAGLRARRLRPLCLRNQQPLGQ*HHLLLR
QCFRCSPLLGG******LLFRGERNR*HQTKPY
ARSSILDIPRKDGKEIACSAGCQDSEVQMPFQWD
PKPHTALVEKWQRIQT*PQNWRLQGPLCHLEHH
NGLCGAL*QGQLHLHCGE*VRQHQPHIPAGCRG
AVPSPAHPASRVARQQNSGPG*QRGVHV*GVQ*P
AAAHPVAKAHRGEWEQDWPRQPALCPDLEDCWS
*YHRQRDGGASLKKCLL*GRRGVYVLGG*LYRT
LPSLCMVDRSGSPGREAGSDDLAPVPGDHHLLHR
GLPHLLHGGVGHRLQDEEWYQEE*LPQPDGCAQ
AGQEHPSAQTGNSVC*LQCIHELWGSSGSAITA
LLQWDSHASRGL*V*ASRRPSLGAASGQTGLRQT
PGRGLLWAGGVGRGYRAGQGQTQPCDQSGCEDV
EVGRNRERLVRPDLRNGDDEDDREA*EYHQPAG
GLHAGWSLVCHRGVCLQGQPAGVPAGPEAPRAGI
LLQPQPQPRGAALLQGPGVLRLPGGPRHGVSGL
QEVHTPRPGSQECPGDRGQCDEDSRLWPRTGHS
PHRLL*KDNQRPTACEVDGTRGII*PDLHPPE*C
VVFRGAPVGDLHSGRLPIPRCACGGTFQAAEGG
SPHGQAQ*LHQRAVHDDAGLLACSALTETHLQA
AGGRPGPHRGLDLQPGVPGPVHAPGPVLPQLSRH
PELYVLLRGGFRLLS*AAARGALPAPTPSPACQ
WRTQTPLTATHTPSPDSTVSCNPHPQPLLGPPP
VRPCPLSCWQEPAAYQGPSCVACLHPTQLTSPST
SSPPAGERCKEADLCCQPLHPLPDVGPTPLPAT
RHCLEGREWEPMNRHASESFLSFLLSVWSVLPS
PISPSHSGGRCLVLRATAVGRSVLRASIEGDLCP
R*VVPVAY*FRY*FALLTKCLVPEDGEAKARLG
AVLWPWGPAPNWGLCI*L*RKHKVYKSEYIFTC
LFKRVVTRDLPIG*DAPGGWEASVAIY*KQKRKK
RKMFLKRSYIFCYFCCFIFLNYVLNLFSV*VPQ
*KLLLL

Copyright ©2008,2017, all rights reserved.