Sequences - Rnomics 📊 Palace

        ☰
    
        Rnomics 📊 Palace
    
                       Back to Rnomics Platform
            
                       Open Session

1 warning:

Access restricted to public data.
Please sign in at Rnomics Platform to access your data.

Navigation

Home

Sequences

Sequence FGFR1.iAug05

CCTCCAACTCGCGCTCGGGAGCTGGCGAGGCGGCGGCGGCTCCTCAGGTCAGTTTGAAAAGGAGGATCGAGCTCACTGTGGAGTATCCATGGAGATGTGG AGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGAGCTGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGCCACACTCTGCACCGCTAGG CCGTCCCCGACCTTGCCTGAACAAGCCCAGCCCTGGGGAGCCCCTGTGGAAGTGGAGTCCTTCCTGGTCCACCCCGGTGACCTGCTGCAGCTTCGCTGTC GGCTGCGGGACGATGTGCAGAGCATCAACTGGCTGCGGGACGGGGTGCAGCTGGCGGAAAGCAACCGCACCCGCATCACAGGGGAGGAGGTGGAGGTGCA GGACTCCGTGCCCGCAGACTCCGGCCTCTATGCTTGCGTAACCAGCAGCCCCTCGGGCAGTGACACCACCTACTTCTCCGTCAATGTTTCAGATGCTCTC CCCTCCTCGGAGGATGATGATGATGATGATGACTCCTCTTCAGAGGAGAAAGAAACAGATAACACCAAACCAAACCCCGTAGCTCCATATTGGACATCCC CAGAAAAGATGGAAAAGAAATTGCATGCAGTGCCGGCTGCCAAGACAGTGAAGTTCAAATGCCCTTCCAGTGGGACCCCAAACCCCACACTGCGCTGGTT GAAAAATGGCAAAGAATTCAAACCTGACCACAGAATTGGAGGCTACAAGGTCCGTTATGCCACCTGGAGCATCATAATGGACTCTGTGGTGCCCTCTGAC AAGGGCAACTACACCTGCATTGTGGAGAATGAGTACGGCAGCATCAACCACACATACCAGCTGGATGTCGTGGAGCGGTCCCCTCACCGGCCCATCCTGC AAGCAGGGTTGCCCGCCAACAAAACAGTGGCCCTGGGTAGCAACGTGGAGTTCATGTGTAAGGTGTACAGTGACCCGCAGCCGCACATCCAGTGGCTAAA GCACATCGAGGTGAATGGGAGCAAGATTGGCCCAGACAACCTGCCTTATGTCCAGATCTTGAAGCATTCGGGGATTAATAGCTCGGATGCGGAGGTGCTG ACCCTGTTCAATGTGACAGAGGCCCAGAGCGGGGAGTATGTGTGTAAGGTTTCCAATTATATTGGTGAAGCTAACCAGTCTGCGTGGCTCACTGTCACCA GACCTGTGGCAAAAGCCCTGGAAGAGAGGCCGGCAGTGATGACCTCGCCCCTGTACCTGGAGATCATCATCTATTGCACAGGGGCCTTCCTCATCTCCTG CATGGTGGGGTCGGTCATCGTCTACAAGATGAAGAGTGGTACCAAGAAGAGTGACTTCCACAGCCAGATGGCTGTGCACAAGCTGGCCAAGAGCATCCCT CTGCGCAGACAGGTAACAGTGTCTGCTGACTCCAGTGCATCCATGAACTCTGGGGTTCTTCTGGTTCGGCCATCACGGCTCTCCTCCAGTGGGACTCCCA TGCTAGCAGGGGTCTCTGAGTATGAGCTTCCCGAAGACCCTCGCTGGGAGCTGCCTCGGGACAGGTAATAATACTTTGACTCCCAACTTTTTGAAGGCAT TCCTTGCTTGCTATTCTAGTGGTGGAAGGTGTGTATACTAACACCTTCTGGGCGGTGAAGGTTCATGAAATGCCTGCAGAAACATTCATTCCACTTTCTG CTCGCAGTTGGGTGAAATACAGAGAGGTGGAGATGGGGTGCCTCTCAAGGTTTGAACTTCACCAGCCCCAACTTATGCCACTCTCTGTTTCCCCCGAAAG ACTGGTCTTAGGCAAACCCCTGGGAGAGGGCTGCTTTGGGCAGGTGGTGTTGGCAGAGGCTATCGGGCTGGACAAGGACAAACCCAACCGTGTGACCAAA GTGGCTGTGAAGATGTTGAAGTGTAAGTGATGCTTCTGTCCTTGCAAAGAAAATCTTGTCCCATTCCAAGCAAACAGCAGGCCTCGGGCCCAGCAGAACA GCTTCTCTGGTGCCTTTGCTGCCCACACCTGCTCCGCTGCCTGCTTGGGGTTACCTGGGAGTGGAGGGGGTGTTCTGCACCTTGAGATTGTTCTATGACG TCACTCTGTTCCTTGGCGTCAACCTGGGCTGTGGTCAGGTGTTTATCCTGGATCCATCGCTTGCAGGCTTTCCTCACCACATCCCTGCCCACTCACTACC ACCTATTGCAACAACGGCTCCCATGAGAGGGAAGAGAAAACAAGTTTGGATGAAGTGGGGAGGAGAGAATCAAGTCCCAGGGAAAAGCAGCCCCTCGACA CATACCCCACTCCCTTAGCCTTTATCCTGCCCTCCTCCCTTCCCAAGTAAATGAGTCTCAACGTGTTCTTTAAAGCGGACGCAACAGAGAAAGACTTGTC AGACCTGATCTCAGAAATGGAGATGATGAAGATGATCGGGAAGCATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTGGGTGCCGGCCA GACTGGCTTTCCCTGCCCAGCGGGGAGAAGATCTGAGCTGGGGTATCAGTCCCAAGGCCTCTCTCAGCCACAGCTAGGGAAGGGGGTTAAGAGAGGCTGC TTTGGCAGAGGTTTAAAATGTGTTATCTCGGGATCTGGGCTCCGCTCATTGGTGTTCATTCAGGAATACAGATACCTAGATTCACACACGTGGTGTGTTA ACAGAGGCAGCTGAGTAATTATGGTCTAAGAGCGCTCAGGCTGGGTGCAGTGGCTCATGCCTGTAATCCCAGCAATTTGGGAGGCCGAGGCAGGAAGATC ACTTGAGATCAGGAGTTCGAGACCAGTCTGGTCAAAATGGTGAAACCCCATCTCTACTAAAAAATACAAAAGTTAGCCAGGCATGGTCACGCATGCCTAT AATCCCAGCTACTCAGGAGGCTGACGCATAAGACTTGCTTGAACCCGGGAGGTGGAGGTTGCAGTGAGCCAAGATCACGCCATTGTACTCCAGCTTGGGA GATACTCCCGCTGTCTCAAAAATAAAATAAATAAATAGGCCAGGCACAGTGGCTCACGCCTGTAATCCCAGCACCTTGGGAGGGTGAGGCGGGTGGATCA CTTGAGGTCAGGAGTTGGAGACCAGCCTGGCCAACAGGTGAAACCCCATCTCTACTAAAAATACAAAAAGTAGCCGGGCGTGGTGGCGCATGCCTGTAAT TCCAGCTACTCGGGAGGCTGTGGCAGGAGAATCGTTTGAACACGGGAGGCGGAGGTTGCGGTTAGCCGAGATCGCGCCATTGCACTCCAGCTTGGGCAAC GAGCAAAACTCCATCTC

FGFR1.F11 (forward primer)
FGFR1.R11 (reverse primer)
Single exon skipping

Frame 0

PPTRARELARRRRLLRSV*KGGSSSLWSIHGDV EPCHQPLTAELGCGAGSASSSGLCWSQPHSAPLG RPRPCLNKPSPGEPLWKWSPSWSTPVTCCSFAV GCGTMCRASTGCGTGCSWRKATAPASQGRRWRC RTPCPQTPASMLA*PAAPRAVTPPTSPSMFQMLS PPRRMMMMMMTPLQRRKKQITPNQTP*LHIGHP QKRWKRNCMQCRLPRQ*SSNALPVGPQTPHCAG *KMAKNSNLTTELEATRSVMPPGAS*WTLWCPLT RATTPALWRMSTAASTTHTSWMSWSGPLTGPSC KQGCPPTKQWPWVATWSSCVRCTVTRSRTSSG* STSR*MGARLAQTTCLMSRS*SIRGLIARMRRC* PCSM*QRPRAGSMCVRFPIILVKLTSLRGSLSP DLWQKPWKRGRQ**PRPCTWRSSSIAQGPSSSP AWWGRSSSTR*RVVPRRVTSTARWLCTSWPRASL CADR*QCLLTPVHP*TLGFFWFGHHGSPPVGLP C*QGSLSMSFPKTLAGSCLGTGNNTLTPNFLKA FLACYSSGGRCVY*HLLGGEGS*NACRNIHSTFC SQLGEIQRGGDGVPLKV*TSPAPTYATLCFPRK TGLRQTPGRGLLWAGGVGRGYRAGQGQTQPCDQ SGCEDVEV*VMLLSLQRKSCPIPSKQQASGPAEQ LLWCLCCPHLLRCLLGVTWEWRGCSAP*DCSMT SLCSLASTWAVVRCLSWIHRLQAFLTTSLPTHY HLLQQRLP*EGRENKFG*SGEERIKSQGKAAPRH IPHSLSLYPALLPSQVNESQRVL*SGRNRERLV RPDLRNGDDEDDREA*EYHQPAGGLHAGWWVPA RLAFPAQRGEDLSWGISPKASLSHS*GRGLREAA LAEV*NVLSRDLGSAHWCSFRNTDT*IHTRGVL TEAAE*LWSKSAQAGCSGSCL*SQQFGRPRQED HLRSGVRDQSGQNGETPSLLKNTKVSQAWSRMPI IPATQEADA*DLLEPGRWRLQ*AKITPLYSSLG DTPAVSKIK*INRPGTVAHACNPSTLGG*GGWI T*GQELETSLANR*NPISTKNTKSSRAWWRMPVI PATREAVAGESFEHGRRRLRLAEIAPLHSSLGN EQNSI

Frame 1

LQLALGSWRGGGGSSGQFEKEDRAHCGVSMEMW SLVTNL*LQNWDVELEVPPLLGCAGHSHTLHR* AVPDLA*TSPALGSPCGSGVLPGPPR*PAAASLS AAGRCAEHQLAAGRGAAGGKQPHPHHRGGGGGA GLRARRLRPLCLRNQQPLGQ*HHLLLRQCFRCS PLLGG******LLFRGERNR*HQTKPRSSILDIP RKDGKEIACSAGCQDSEVQMPFQWDPKPHTALV EKWQRIQT*PQNWRLQGPLCHLEHHNGLCGAL* QGQLHLHCGE*VRQHQPHIPAGCRGAVPSPAHPA SRVARQQNSGPG*QRGVHV*GVQ*PAAAHPVAK AHRGEWEQDWPRQPALCPDLEAFGD**LGCGGA DPVQCDRGPERGVCV*GFQLYW*S*PVCVAHCHQ TCGKSPGREAGSDDLAPVPGDHHLLHRGLPHLL HGGVGHRLQDEEWYQEE*LPQPDGCAQAGQEHP SAQTGNSVC*LQCIHELWGSSGSAITALLQWDSH ASRGL*V*ASRRPSLGAASGQVIIL*LPTF*RH SLLAILVVEGVYTNTFWAVKVHEMPAETFIPLS ARSWVKYREVEMGCLSRFELHQPQLMPLSVSPER LVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTK VAVKMLKCK*CFCPCKENLVPFQANSRPRAQQN SFSGAFAAHTCSAACLGLPGSGGGVLHLEIVL*R HSVPWRQPGLWSGVYPGSIACRLSSPHPCPLTT TYCNNGSHEREEKTSLDEVGRRESSPREKQPLD TYPTPLAFILPSSLPK*MSLNVFFKADATEKDLS DLISEMEMMKMIGKHKNIINLLGACTQDGGCRP DWLSLPSGEKI*AGVSVPRPLSATAREGG*ERL LWQRFKMCYLGIWAPLIGVHSGIQIPRFTHVVC* QRQLSNYGLRALRLGAVAHACNPSNLGGRGRKI T*DQEFETSLVKMVKPHLY*KIQKLARHGHACL *SQLLRRLTHKTCLNPGGGGCSEPRSRHCTPAWE ILPLSQK*NK*IGQAQWLTPVIPAPWEGEAGGS LEVRSWRPAWPTGETPSLLKIQKVAGRGGACL* FQLLGRLWQENRLNTGGGGCG*PRSRHCTPAWAT SKTPS

Frame 2

SNSRSGAGEAAAAPQVSLKRRIELTVEYPWRCG ALSPTSNCRTGMWSWKCLLFWAVLVTATLCTAR PSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRC RLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQ DSVPADSGLYACVTSSPSGSDTTYFSVNVSDAL PSSEDDDDDDDSSSEEKETDNTKPNPVAPYWTS PEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWL KNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSD KGNYTCIVENEYGSINHTYQLDVVERSPHRPIL QAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLK HIEVNGSKIGPDNLPYVQILKHSGINSSDAEVL TLFNVTEAQSGEYVCKVSNYIGEANQSAWLTVT RPVAKALEERPAVMTSPLYLEIIIYCTGAFLISC MVGSVIVYKMKSGTKKSDFHSQMAVHKLAKSIP LRRQVTVSADSSASMNSGVLLVRPSRLSSSGTP MLAGVSEYELPEDPRWELPRDR**YFDSQLFEGI PCLLF*WWKVCILTPSGR*RFMKCLQKHSFHFL LAVG*NTERWRWGASQGLNFTSPNLCHSLFPPK DWS*ANPWERAALGRWCWQRLSGWTRTNPTV*PK WL*RC*SVSDASVLAKKILSHSKQTAGLGPSRT ASLVPLLPTPAPLPAWGYLGVEGVFCTLRLFYD VTLFLGVNLGCGQVFILDPSLAGFPHHIPAHSLP PIATTAPMRGKRKQVWMKWGGENQVPGKSSPST HTPLP*PLSCPPPFPSK*VSTCSLKRTQQRKTC QT*SQKWR**R*SGSIRISSTCWGPARRMVGAGQ TGFPCPAGRRSELGYQSQGLSQPQLGKGVKRGC FGRGLKCVISGSGLRSLVFIQEYRYLDSHTWCV NRGS*VIMV*ERSGWVQWLMPVIPAIWEAEAGRS LEIRSSRPVWSKW*NPISTKKYKS*PGMVTHAY NPSYSGG*RIRLA*TREVEVAVSQDHAIVLQLG RYSRCLKNKINK*ARHSGSRL*SQHLGRVRRVDH LRSGVGDQPGQQVKPHLY*KYKK*PGVVAHACN SSYSGGCGRRIV*TREAEVAVSRDRAIALQLGQ RAKLHL

Sequence FGFR1.fAug05

CCCCGCCGGCGCATAGCGCTCGGAGCGCTCTTGCGGCCACAGGCGCGGCGTCCTCGGCGGCGGGCGGCAGCTAGCGGGAGCCGGGACGCCGGTGCAGCCG CAGCGCGCGGAGGAACCCGGGTGTGCCGGGAGCTGGGCGGCCACGTCCGGACGGGACCGAGACCCCTCGTAGCGCATTGCGGCGACCTCGCCTTCCCCGG CCGCGAGCGCGCCGCTGCTTGAAAAGCCGCGGAACCCAAGGACTTTTCTCCGGTCCGAGCTCGGGGCGCCCCGCAGGGCGCACGGTACCCGTGCTGCAGT CGGGCACGCCGCGGCGCCGGGGCCTCCGCAGGGCGATGGAGCCCGGTCTGCAAGGAAAGTGAGGCGCCGCCGCTGCGTTCTGGAGGAGGGGGGCACAAGG TCTGGAGACCCCGGGTGGCGGACGGGAGCCCTCCCCCCGCCCCGCCTCCGGGGCACCAGCTCCGGCTCCATTGTTCCCGCCCGGGCTGGAGGCGCCGAGC ACCGAGCGCCGCCGGGAGTCGAGCGCCGGCCGCGGAGCTCTTGCGACCCCGCCAGGACCCGAACAGAGCCCGGGGGCGGCGGGCCGGAGCCGGGGACGCG GGCACACGCCCGCTCGCACAAGCCACGGCGGACTCTCCCGAGGCGGAACCTCCACGCCGAGCGAGGGTCAGTTTGAAAAGGAGGATCGAGCTCACTGTGG AGTATCCATGGAGATGTGGAGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGAGCTGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGC CACACTCTGCACCGCTAGGCCGTCCCCGACCTTGCCTGAACAAGCCCAGCCCTGGGGAGCCCCTGTGGAAGTGGAGTCCTTCCTGGTCCACCCCGGTGAC CTGCTGCAGCTTCGCTGTCGGCTGCGGGACGATGTGCAGAGCATCAACTGGCTGCGGGACGGGGTGCAGCTGGCGGAAAGCAACCGCACCCGCATCACAG GGGAGGAGGTGGAGGTGCAGGACTCCGTGCCCGCAGACTCCGGCCTCTATGCTTGCGTAACCAGCAGCCCCTCGGGCAGTGACACCACCTACTTCTCCGT CAATGTTTCAGATGCTCTCCCCTCCTCGGAGGATGATGATGATGATGATGACTCCTCTTCAGAGGAGAAAGAAACAGATAACACCAAACCAAACCGTATG CCCGTAGCTCCATATTGGACATCCCCAGAAAAGATGGAAAAGAAATTGCATGCAGTGCCGGCTGCCAAGACAGTGAAGTTCAAATGCCCTTCCAGTGGGA CCCCAAACCCCACACTGCGCTGGTTGAAAAATGGCAAAGAATTCAAACCTGACCACAGAATTGGAGGCTACAAGGTCCGTTATGCCACCTGGAGCATCAT AATGGACTCTGTGGTGCCCTCTGACAAGGGCAACTACACCTGCATTGTGGAGAATGAGTACGGCAGCATCAACCACACATACCAGCTGGATGTCGTGGAG CGGTCCCCTCACCGGCCCATCCTGCAAGCAGGGTTGCCCGCCAACAAAACAGTGGCCCTGGGTAGCAACGTGGAGTTCATGTGTAAGGTGTACAGTGACC CGCAGCCGCACATCCAGTGGCTAAAGCACATCGAGGTGAATGGGAGCAAGATTGGCCCAGACAACCTGCCTTATGTCCAGATCTTGAAGACTGCTGGAGT TAATACCACCGACAAAGAGATGGAGGTGCTTCACTTAAGAAATGTCTCCTTTGAGGACGCAGGGGAGTATACGTGCTTGGCGGGTAACTCTATCGGACTC TCCCATCACTCTGCATGGTTGACCGTTCTGGAAGCCCTGGAAGAGAGGCCGGCAGTGATGACCTCGCCCCTGTACCTGGAGATCATCATCTATTGCACAG GGGCCTTCCTCATCTCCTGCATGGTGGGGTCGGTCATCGTCTACAAGATGAAGAGTGGTACCAAGAAGAGTGACTTCCACAGCCAGATGGCTGTGCACAA GCTGGCCAAGAGCATCCCTCTGCGCAGACAGGTAACAGTGTCTGCTGACTCCAGTGCATCCATGAACTCTGGGGTTCTTCTGGTTCGGCCATCACGGCTC TCCTCCAGTGGGACTCCCATGCTAGCAGGGGTCTCTGAGTATGAGCTTCCCGAAGACCCTCGCTGGGAGCTGCCTCGGGACAGACTGGTCTTAGGCAAAC CCCTGGGAGAGGGCTGCTTTGGGCAGGTGGTGTTGGCAGAGGCTATCGGGCTGGACAAGGACAAACCCAACCGTGTGACCAAAGTGGCTGTGAAGATGTT GAAGTCGGACGCAACAGAGAAAGACTTGTCAGACCTGATCTCAGAAATGGAGATGATGAAGATGATCGGGAAGCATAAGAATATCATCAACCTGCTGGGG GCCTGCACGCAGGATGGTCCCTTGTATGTCATCGTGGAGTATGCCTCCAAGGGCAACCTGCGGGAGTACCTGCAGGCCCGGAGGCCCCCAGGGCTGGAAT ACTGCTACAACCCCAGCCACAACCCAGAGGAGCAGCTCTCCTCCAAGGACCTGGTGTCCTGCGCCTACCAGGTGGCCCGAGGCATGGAGTATCTGGCCTC CAAGAAGGTGTGGAACCTGAAGGCTCCCCTGGTGCATACACCGAGACCTGGCAGCCAGGAATGTCCTGGTGACAGAGGACAATGTGATGAAGATAGCAGA CTTTGGCCTCGCACGGGACATTCACCACATCGACTACTATAAAAAGACAACCAACGGCCGACTGCCTGTGAAGTGGATGGCACCCGAGGCATTATTTGAC CGGATCTACACCCACCAGAGTGATGTGTGGTCTTTCGGGGTGCTCCTGTGGGAGATCTTCACTCTGGGCGGCTCCCCATACCCCGGTGTGCCTGTGGAGG AACTTTTCAAGCTGCTGAAGGAGGGTCACCGCATGGACAAGCCCAGTAACTGCACCAACGAGCTGTACATGATGATGCGGGACTGCTGGCATGCAGTGCC CTCACAGAGACCCACCTTCAAGCAGCTGGTGGAAGACCTGGACCGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCCCCTGGAC CAGTACTCCCCCAGCTTTCCCGACACCCGGAGCTCTACGTGCTCCTCAGGGGAGGATTCCGTCTTCTCTCATGAGCCGCTGCCCGAGGAGCCCTGCCTGC CCCGACACCCAGCCCAGCTTGCCAATGGCGGACTCAAACGCCGCTGACTGCCACCCACACGCCCTCCCCAGACTCCACCGTCAGCTGTAACCCTCACCCA CAGCCCCTGCTGGGCCCACCACCTGTCCGTCCCTGTCCCCTTTCCTGCTGGCAGGAGCCGGCTGCCTACCAGGGGCCTTCCTGTGTGGCCTGCCTTCACC CCACTCAGCTCACCTCTCCCTCCACCTCCTCTCCACCTGCTGGTGAGAGGTGCAAAGAGGCAGATCTTTGCTGCCAGCCACTTCATCCCCTCCCAGATGT TGGACCAACACCCCTCCCTGCCACCAGGCACTGCCTGGAGGGCAGGGAGTGGGAGCCAATGAACAGGCATGCAAGTGAGAGCTTCCTGAGCTTTCTCCTG TCGGTTTGGTCTGTTTTGCCTTCACCCATAAGCCCCTCGCACTCTGGTGGCAGGTGCCTTGTCCTCAGGGCTACAGCAGTAGGGAGGTCAGTGCTTCGTG CCTCGATTGAAGGTGACCTCTGCCCCAGATAGGTGGTGCCAGTGGCTTATTAATTCCGATACTAGTTTGCTTTGCTGACCAAATGCCTGGTACCAGAGGA TGGTGAGGCGAAGGCCAGGTTGGGGGCAGTGTTGTGGCCCTGGGGCCCAGCCCCAAACTGGGGGCTCTGTATATAGCTATGAAGAAAACACAAAGTGTAT AAATCTGAGTATATATTTACATGTCTTTTTAAAAGGGTCGTTACCAGAGATTTACCCATCGGGTAAGATGCTCCTGGTGGCTGGGAGGCATCAGTTGCTA TATATTAAAAACAAAAAAGAAAAAAAAGGAAAATGTTTTTAAAAAGGTCATATATTTTTTGCTACTTTTGCTGTTTTATTTTTTTAAATTATGTTCTAAA CCTATTTTCAGTTTAGGTCCCTCAATAAAAATTGCTGCTGCTTC

FGFR1.F11 (forward primer)
FGFR1.R11 (reverse primer)
Single exon skipping

Frame 0

PRRRIALGALLRPQARRPRRRAAASGSRDAGAA AARGGTRVCRELGGHVRTGPRPLVAHCGDLAFPG RERAAA*KAAEPKDFSPVRARGAPQGARYPCCS RARRGAGASAGRWSPVCKESEAPPLRSGGGGHK VWRPRVADGSPPPAPPPGHQLRLHCSRPGWRRRA PSAAGSRAPAAELLRPRQDPNRARGRRAGAGDA GTRPLAQATADSPEAEPPRRARVSLKRRIELTV EYPWRCGALSPTSNCRTGMWSWKCLLFWAVLVTA TLCTARPSPTLPEQAQPWGAPVEVESFLVHPGD LLQLRCRLRDDVQSINWLRDGVQLAESNRTRIT GEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSV NVSDALPSSEDDDDDDDSSSEEKETDNTKPNRM PVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSG TPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSII MDSVVPSDKGNYTCIVENEYGSINHTYQLDVVE RSPHRPILQAGLPANKTVALGSNVEFMCKVYSD PQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGV NTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGL SHHSAWLTVLEALEERPAVMTSPLYLEIIIYCT GAFLISCMVGSVIVYKMKSGTKKSDFHSQMAVHK LAKSIPLRRQVTVSADSSASMNSGVLLVRPSRL SSSGTPMLAGVSEYELPEDPRWELPRDRLVLGK PLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKML KSDATEKDLSDLISEMEMMKMIGKHKNIINLLG ACTQDGPLYVIVEYASKGNLREYLQARRPPGLE YCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLAS KKVWNLKAPLVHTPRPGSQECPGDRGQCDEDSR LWPRTGHSPHRLL*KDNQRPTACEVDGTRGII* PDLHPPE*CVVFRGAPVGDLHSGRLPIPRCACGG TFQAAEGGSPHGQAQ*LHQRAVHDDAGLLACSA LTETHLQAAGGRPGPHRGLDLQPGVPGPVHAPG PVLPQLSRHPELYVLLRGGFRLLS*AAARGALPA PTPSPACQWRTQTPLTATHTPSPDSTVSCNPHP QPLLGPPPVRPCPLSCWQEPAAYQGPSCVACLH PTQLTSPSTSSPPAGERCKEADLCCQPLHPLPDV GPTPLPATRHCLEGREWEPMNRHASESFLSFLL SVWSVLPSPISPSHSGGRCLVLRATAVGRSVLR ASIEGDLCPR*VVPVAY*FRY*FALLTKCLVPED GEAKARLGAVLWPWGPAPNWGLCI*L*RKHKVY KSEYIFTCLFKRVVTRDLPIG*DAPGGWEASVA IY*KQKRKKRKMFLKRSYIFCYFCCFIFLNYVLN LFSV*VPQ*KLLLL

Frame 1

PAGA*RSERSCGHRRGVLGGGRQLAGAGTPVQP QRAEEPGCAGSWAATSGRDRDPS*RIAATSPSP AASAPLLEKPRNPRTFLRSELGAPRRAHGTRAAV GHAAAPGPPQGDGARSARKVRRRRCVLEEGGTR SGDPGWRTGALPPPRLRGTSSGSIVPARAGGAE HRAPPGVERRPRSSCDPARTRTEPGGGGPEPGTR AHARSHKPRRTLPRRNLHAERGSV*KGGSSSLW SIHGDVEPCHQPLTAELGCGAGSASSSGLCWSQ PHSAPLGRPRPCLNKPSPGEPLWKWSPSWSTPVT CCSFAVGCGTMCRASTGCGTGCSWRKATAPASQ GRRWRCRTPCPQTPASMLA*PAAPRAVTPPTSP SMFQMLSPPRRMMMMMMTPLQRRKKQITPNQTVC P*LHIGHPQKRWKRNCMQCRLPRQ*SSNALPVG PQTPHCAG*KMAKNSNLTTELEATRSVMPPGAS *WTLWCPLTRATTPALWRMSTAASTTHTSWMSWS GPLTGPSCKQGCPPTKQWPWVATWSSCVRCTVT RSRTSSG*STSR*MGARLAQTTCLMSRS*RLLE LIPPTKRWRCFT*EMSPLRTQGSIRAWRVTLSDS PITLHG*PFWKPWKRGRQ**PRPCTWRSSSIAQ GPSSSPAWWGRSSSTR*RVVPRRVTSTARWLCT SWPRASLCADR*QCLLTPVHP*TLGFFWFGHHGS PPVGLPC*QGSLSMSFPKTLAGSCLGTDWS*AN PWERAALGRWCWQRLSGWTRTNPTV*PKWL*RC *SRTQQRKTCQT*SQKWR**R*SGSIRISSTCWG PARRMVPCMSSWSMPPRATCGSTCRPGGPQGWN TATTPATTQRSSSPPRTWCPAPTRWPEAWSIWP PRRCGT*RLPWCIHRDLAARNVLVTEDNVMKIAD FGLARDIHHIDYYKKTTNGRLPVKWMAPEALFD RIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVE ELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVP SQRPTFKQLVEDLDRIVALTSNQEYLDLSMPLD QYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCL PRHPAQLANGGLKRR*LPPTRPPQTPPSAVTLTH SPCWAHHLSVPVPFPAGRSRLPTRGLPVWPAFT PLSSPLPPPPLHLLVRGAKRQIFAASHFIPSQM LDQHPSLPPGTAWRAGSGSQ*TGMQVRAS*AFSC RFGLFCLHP*APRTLVAGALSSGLQQ*GGQCFV PRLKVTSAPDRWCQWLINSDTSLLC*PNAWYQR MVRRRPGWGQCCGPGAQPQTGGSVYSYEENTKCI NLSIYLHVFLKGSLPEIYPSGKMLLVAGRHQLL YIKNKKEKKGKCF*KGHIFFATFAVLFF*IMF* TYFQFRSLNKNCCCF

Frame 2

PPAHSARSALAATGAASSAAGGS*REPGRRCSR SARRNPGVPGAGRPRPDGTETPRSALRRPRLPR PRARRCLKSRGTQGLFSGPSSGRPAGRTVPVLQ SGTPRRRGLRRAMEPGLQGK*GAAAAFWRRGAQG LETPGGGREPSPRPASGAPAPAPLFPPGLEAPS TERRRESSAGRGALATPPGPEQSPGAAGRSRGR GHTPARTSHGGLSRGGTSTPSEGQFEKEDRAHCG VSMEMWSLVTNL*LQNWDVELEVPPLLGCAGHS HTLHR*AVPDLA*TSPALGSPCGSGVLPGPPR* PAAASLSAAGRCAEHQLAAGRGAAGGKQPHPHHR GGGGGAGLRARRLRPLCLRNQQPLGQ*HHLLLR QCFRCSPLLGG******LLFRGERNR*HQTKPY ARSSILDIPRKDGKEIACSAGCQDSEVQMPFQWD PKPHTALVEKWQRIQT*PQNWRLQGPLCHLEHH NGLCGAL*QGQLHLHCGE*VRQHQPHIPAGCRG AVPSPAHPASRVARQQNSGPG*QRGVHV*GVQ*P AAAHPVAKAHRGEWEQDWPRQPALCPDLEDCWS *YHRQRDGGASLKKCLL*GRRGVYVLGG*LYRT LPSLCMVDRSGSPGREAGSDDLAPVPGDHHLLHR GLPHLLHGGVGHRLQDEEWYQEE*LPQPDGCAQ AGQEHPSAQTGNSVC*LQCIHELWGSSGSAITA LLQWDSHASRGL*V*ASRRPSLGAASGQTGLRQT PGRGLLWAGGVGRGYRAGQGQTQPCDQSGCEDV EVGRNRERLVRPDLRNGDDEDDREA*EYHQPAG GLHAGWSLVCHRGVCLQGQPAGVPAGPEAPRAGI LLQPQPQPRGAALLQGPGVLRLPGGPRHGVSGL QEGVEPEGSPGAYTETWQPGMSW*QRTM**R*Q TLASHGTFTTSTTIKRQPTADCL*SGWHPRHYLT GSTPTRVMCGLSGCSCGRSSLWAAPHTPVCLWR NFSSC*RRVTAWTSPVTAPTSCT**CGTAGMQC PHRDPPSSSWWKTWTASWP*PPTRSTWTCPCPWT STPPAFPTPGALRAPQGRIPSSLMSRCPRSPAC PDTQPSLPMADSNAADCHPHALPRLHRQL*PSP TAPAGPTTCPSLSPFLLAGAGCLPGAFLCGLPSP HSAHLSLHLLSTCW*EVQRGRSLLPATSSPPRC WTNTPPCHQALPGGQGVGANEQACK*ELPELSP VGLVCFAFTHKPLALWWQVPCPQGYSSREVSASC LD*R*PLPQIGGASGLLIPILVCFADQMPGTRG W*GEGQVGGSVVALGPSPKLGALYIAMKKTQSV *I*VYIYMSF*KGRYQRFTHRVRCSWWLGGISCY ILKTKKKKKENVFKKVIYFLLLLLFYFFKLCSK PIFSLGPSIKIAAA

Sequence FGFR1.cAug05

CCCCGCCGGCGCATAGCGCTCGGAGCGCTCTTGCGGCCACAGGCGCGGCGTCCTCGGCGGCGGGCGGCAGCTAGCGGGAGCCGGGACGCCGGTGCAGCCG CAGCGCGCGGAGGAACCCGGGTGTGCCGGGAGCTGGGCGGCCACGTCCGGACGGGACCGAGACCCCTCGTAGCGCATTGCGGCGACCTCGCCTTCCCCGG CCGCGAGCGCGCCGCTGCTTGAAAAGCCGCGGAACCCAAGGACTTTTCTCCGGTCCGAGCTCGGGGCGCCCCGCAGGGCGCACGGTACCCGTGCTGCAGT CGGGCACGCCGCGGCGCCGGGGCCTCCGCAGGGCGATGGAGCCCGGTCTGCAAGGAAAGTGAGGCGCCGCCGCTGCGTTCTGGAGGAGGGGGGCACAAGC TCCGGCTCCATTGTTCCCGCCCGGGCTGGAGGCGCCGAGCACCGAGCGCCGCCGGGAGTCGAGCGCCGGCCGCGGAGCTCTTGCGACCCCGCCAGGACCC GAACAGAGCCCGGGGGCGGCGGGCCGGAGCCGGGGACGCGGGCACACGCCCGCTCGCACAAGCCACGGCGGACTCTCCCGAGGCGGAACCTCCACGCCGA GCGAGGGTCAGTTTGAAAAGGAGGATCGAGCTCACTGTGGAGTATCCATGGAGATGTGGAGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGA GCTGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGCCACACTCTGCACCGCTAGGCCGTCCCCGACCTTGCCTGAACAAGCCCAGCCCTGGGGAGC CCCTGTGGAAGTGGAGTCCTTCCTGGTCCACCCCGGTGACCTGCTGCAGCTTCGCTGTCGGCTGCGGGACGATGTGCAGAGCATCAACTGGCTGCGGGAC GGGGTGCAGCTGGCGGAAAGCAACCGCACCCGCATCACAGGGGAGGAGGTGGAGGTGCAGGACTCCGTGCCCGCAGACTCCGGCCTCTATGCTTGCGTAA CCAGCAGCCCCTCGGGCAGTGACACCACCTACTTCTCCGTCAATGTTTCAGATGCTCTCCCCTCCTCGGAGGATGATGATGATGATGATGACTCCTCTTC AGAGGAGAAAGAAACAGATAACACCAAACCAAACCGTATGCCCGTAGCTCCATATTGGACATCCCCAGAAAAGATGGAAAAGAAATTGCATGCAGTGCCG GCTGCCAAGACAGTGAAGTTCAAATGCCCTTCCAGTGGGACCCCAAACCCCACACTGCGCTGGTTGAAAAATGGCAAAGAATTCAAACCTGACCACAGAA TTGGAGGCTACAAGGTCCGTTATGCCACCTGGAGCATCATAATGGACTCTGTGGTGCCCTCTGACAAGGGCAACTACACCTGCATTGTGGAGAATGAGTA CGGCAGCATCAACCACACATACCAGCTGGATGTCGTGGAGCGGTCCCCTCACCGGCCCATCCTGCAAGCAGGGTTGCCCGCCAACAAAACAGTGGCCCTG GGTAGCAACGTGGAGTTCATGTGTAAGGTGTACAGTGACCCGCAGCCGCACATCCAGTGGCTAAAGCACATCGAGGTGAATGGGAGCAAGATTGGCCCAG ACAACCTGCCTTATGTCCAGATCTTGAAGACTGCTGGAGTTAATACCACCGACAAAGAGATGGAGGTGCTTCACTTAAGAAATGTCTCCTTTGAGGACGC AGGGGAGTATACGTGCTTGGCGGGTAACTCTATCGGACTCTCCCATCACTCTGCATGGTTGACCGTTCTGGAAGCCCTGGAAGAGAGGCCGGCAGTGATG ACCTCGCCCCTGTACCTGGAGATCATCATCTATTGCACAGGGGCCTTCCTCATCTCCTGCATGGTGGGGTCGGTCATCGTCTACAAGATGAAGAGTGGTA CCAAGAAGAGTGACTTCCACAGCCAGATGGCTGTGCACAAGCTGGCCAAGAGCATCCCTCTGCGCAGACAGGTGTCTGCTGACTCCAGTGCATCCATGAA CTCTGGGGTTCTTCTGGTTCGGCCATCACGGCTCTCCTCCAGTGGGACTCCCATGCTAGCAGGGGTCTCTGAGTATGAGCTTCCCGAAGACCCTCGCTGG GAGCTGCCTCGGGACAGACTGGTCTTAGGCAAACCCCTGGGAGAGGGCTGCTTTGGGCAGGTGGTGTTGGCAGAGGCTATCGGGCTGGACAAGGACAAAC CCAACCGTGTGACCAAAGTGGCTGTGAAGATGTTGAAGTCGGACGCAACAGAGAAAGACTTGTCAGACCTGATCTCAGAAATGGAGATGATGAAGATGAT CGGGAAGCATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTTGTATGTCATCGTGGAGTATGCCTCCAAGGGCAACCTGCGGGAG TACCTGCAGGCCCGGAGGCCCCCAGGGCTGGAATACTGCTACAACCCCAGCCACAACCCAGAGGAGCAGCTCTCCTCCAAGGACCTGGTGTCCTGCGCCT ACCAGGTGGCCCGAGGCATGGAGTATCTGGCCTCCAAGAAGTGCATACACCGAGACCTGGCAGCCAGGAATGTCCTGGTGACAGAGGACAATGTGATGAA GATAGCAGACTTTGGCCTCGCACGGGACATTCACCACATCGACTACTATAAAAAGACAACCAACGGCCGACTGCCTGTGAAGTGGATGGCACCCGAGGCA TTATTTGACCGGATCTACACCCACCAGAGTGATGTGTGGTCTTTCGGGGTGCTCCTGTGGGAGATCTTCACTCTGGGCGGCTCCCCATACCCCGGTGTGC CTGTGGAGGAACTTTTCAAGCTGCTGAAGGAGGGTCACCGCATGGACAAGCCCAGTAACTGCACCAACGAGCTGTACATGATGATGCGGGACTGCTGGCA TGCAGTGCCCTCACAGAGACCCACCTTCAAGCAGCTGGTGGAAGACCTGGACCGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATG CCCCTGGACCAGTACTCCCCCAGCTTTCCCGACACCCGGAGCTCTACGTGCTCCTCAGGGGAGGATTCCGTCTTCTCTCATGAGCCGCTGCCCGAGGAGC CCTGCCTGCCCCGACACCCAGCCCAGCTTGCCAATGGCGGACTCAAACGCCGCTGACTGCCACCCACACGCCCTCCCCAGACTCCACCGTCAGCTGTAAC CCTCACCCACAGCCCCTGCTGGGCCCACCACCTGTCCGTCCCTGTCCCCTTTCCTGCTGGCAGGAGCCGGCTGCCTACCAGGGGCCTTCCTGTGTGGCCT GCCTTCACCCCACTCAGCTCACCTCTCCCTCCACCTCCTCTCCACCTGCTGGTGAGAGGTGCAAAGAGGCAGATCTTTGCTGCCAGCCACTTCATCCCCT CCCAGATGTTGGACCAACACCCCTCCCTGCCACCAGGCACTGCCTGGAGGGCAGGGAGTGGGAGCCAATGAACAGGCATGCAAGTGAGAGCTTCCTGAGC TTTCTCCTGTCGGTTTGGTCTGTTTTGCCTTCACCCATAAGCCCCTCGCACTCTGGTGGCAGGTGCCTTGTCCTCAGGGCTACAGCAGTAGGGAGGTCAG TGCTTCGTGCCTCGATTGAAGGTGACCTCTGCCCCAGATAGGTGGTGCCAGTGGCTTATTAATTCCGATACTAGTTTGCTTTGCTGACCAAATGCCTGGT ACCAGAGGATGGTGAGGCGAAGGCCAGGTTGGGGGCAGTGTTGTGGCCCTGGGGCCCAGCCCCAAACTGGGGGCTCTGTATATAGCTATGAAGAAAACAC AAAGTGTATAAATCTGAGTATATATTTACATGTCTTTTTAAAAGGGTCGTTACCAGAGATTTACCCATCGGGTAAGATGCTCCTGGTGGCTGGGAGGCAT CAGTTGCTATATATTAAAAACAAAAAAGAAAAAAAAGGAAAATGTTTTTAAAAAGGTCATATATTTTTTGCTACTTTTGCTGTTTTATTTTTTTAAATTA TGTTCTAAACCTATTTTCAGTTTAGGTCCCTCAATAAAAATTGCTGCTGCTTC

FGFR1.F11 (forward primer)
FGFR1.R11 (reverse primer)
Single exon skipping

Frame 0

PRRRIALGALLRPQARRPRRRAAASGSRDAGAA AARGGTRVCRELGGHVRTGPRPLVAHCGDLAFPG RERAAA*KAAEPKDFSPVRARGAPQGARYPCCS RARRGAGASAGRWSPVCKESEAPPLRSGGGGHK LRLHCSRPGWRRRAPSAAGSRAPAAELLRPRQDP NRARGRRAGAGDAGTRPLAQATADSPEAEPPRR ARVSLKRRIELTVEYPWRCGALSPTSNCRTGMW SWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGA PVEVESFLVHPGDLLQLRCRLRDDVQSINWLRD GVQLAESNRTRITGEEVEVQDSVPADSGLYACV TSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSS EEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVP AAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHR IGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEY GSINHTYQLDVVERSPHRPILQAGLPANKTVAL GSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGP DNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDA GEYTCLAGNSIGLSHHSAWLTVLEALEERPAVM TSPLYLEIIIYCTGAFLISCMVGSVIVYKMKSG TKKSDFHSQMAVHKLAKSIPLRRQVSADSSASMN SGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRW ELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDK PNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMI GKHKNIINLLGACTQDGPLYVIVEYASKGNLRE YLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCA YQVARGMEYLASKKCIHRDLAARNVLVTEDNVMK IADFGLARDIHHIDYYKKTTNGRLPVKWMAPEA LFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGV PVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWH AVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSM PLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEE PCLPRHPAQLANGGLKRR*LPPTRPPQTPPSAVT LTHSPCWAHHLSVPVPFPAGRSRLPTRGLPVWP AFTPLSSPLPPPPLHLLVRGAKRQIFAASHFIP SQMLDQHPSLPPGTAWRAGSGSQ*TGMQVRAS*A FSCRFGLFCLHP*APRTLVAGALSSGLQQ*GGQ CFVPRLKVTSAPDRWCQWLINSDTSLLC*PNAW YQRMVRRRPGWGQCCGPGAQPQTGGSVYSYEENT KCINLSIYLHVFLKGSLPEIYPSGKMLLVAGRH QLLYIKNKKEKKGKCF*KGHIFFATFAVLFF*I MF*TYFQFRSLNKNCCCF

Frame 1

PAGA*RSERSCGHRRGVLGGGRQLAGAGTPVQP QRAEEPGCAGSWAATSGRDRDPS*RIAATSPSP AASAPLLEKPRNPRTFLRSELGAPRRAHGTRAAV GHAAAPGPPQGDGARSARKVRRRRCVLEEGGTS SGSIVPARAGGAEHRAPPGVERRPRSSCDPART RTEPGGGGPEPGTRAHARSHKPRRTLPRRNLHAE RGSV*KGGSSSLWSIHGDVEPCHQPLTAELGCG AGSASSSGLCWSQPHSAPLGRPRPCLNKPSPGE PLWKWSPSWSTPVTCCSFAVGCGTMCRASTGCGT GCSWRKATAPASQGRRWRCRTPCPQTPASMLA* PAAPRAVTPPTSPSMFQMLSPPRRMMMMMMTPL QRRKKQITPNQTVCP*LHIGHPQKRWKRNCMQCR LPRQ*SSNALPVGPQTPHCAG*KMAKNSNLTTE LEATRSVMPPGAS*WTLWCPLTRATTPALWRMS TAASTTHTSWMSWSGPLTGPSCKQGCPPTKQWPW VATWSSCVRCTVTRSRTSSG*STSR*MGARLAQ TTCLMSRS*RLLELIPPTKRWRCFT*EMSPLRT QGSIRAWRVTLSDSPITLHG*PFWKPWKRGRQ** PRPCTWRSSSIAQGPSSSPAWWGRSSSTR*RVV PRRVTSTARWLCTSWPRASLCADRCLLTPVHP* TLGFFWFGHHGSPPVGLPC*QGSLSMSFPKTLAG SCLGTDWS*ANPWERAALGRWCWQRLSGWTRTN PTV*PKWL*RC*SRTQQRKTCQT*SQKWR**R* SGSIRISSTCWGPARRMVPCMSSWSMPPRATCGS TCRPGGPQGWNTATTPATTQRSSSPPRTWCPAP TRWPEAWSIWPPRSAYTETWQPGMSW*QRTM** R*QTLASHGTFTTSTTIKRQPTADCL*SGWHPRH YLTGSTPTRVMCGLSGCSCGRSSLWAAPHTPVC LWRNFSSC*RRVTAWTSPVTAPTSCT**CGTAG MQCPHRDPPSSSWWKTWTASWP*PPTRSTWTCPC PWTSTPPAFPTPGALRAPQGRIPSSLMSRCPRS PACPDTQPSLPMADSNAADCHPHALPRLHRQL* PSPTAPAGPTTCPSLSPFLLAGAGCLPGAFLCGL PSPHSAHLSLHLLSTCW*EVQRGRSLLPATSSP PRCWTNTPPCHQALPGGQGVGANEQACK*ELPE LSPVGLVCFAFTHKPLALWWQVPCPQGYSSREVS ASCLD*R*PLPQIGGASGLLIPILVCFADQMPG TRGW*GEGQVGGSVVALGPSPKLGALYIAMKKT QSV*I*VYIYMSF*KGRYQRFTHRVRCSWWLGGI SCYILKTKKKKKENVFKKVIYFLLLLLFYFFKL CSKPIFSLGPSIKIAAA

Frame 2

PPAHSARSALAATGAASSAAGGS*REPGRRCSR SARRNPGVPGAGRPRPDGTETPRSALRRPRLPR PRARRCLKSRGTQGLFSGPSSGRPAGRTVPVLQ SGTPRRRGLRRAMEPGLQGK*GAAAAFWRRGAQA PAPLFPPGLEAPSTERRRESSAGRGALATPPGP EQSPGAAGRSRGRGHTPARTSHGGLSRGGTSTP SEGQFEKEDRAHCGVSMEMWSLVTNL*LQNWDVE LEVPPLLGCAGHSHTLHR*AVPDLA*TSPALGS PCGSGVLPGPPR*PAAASLSAAGRCAEHQLAAG RGAAGGKQPHPHHRGGGGGAGLRARRLRPLCLRN QQPLGQ*HHLLLRQCFRCSPLLGG******LLF RGERNR*HQTKPYARSSILDIPRKDGKEIACSA GCQDSEVQMPFQWDPKPHTALVEKWQRIQT*PQN WRLQGPLCHLEHHNGLCGAL*QGQLHLHCGE*V RQHQPHIPAGCRGAVPSPAHPASRVARQQNSGP G*QRGVHV*GVQ*PAAAHPVAKAHRGEWEQDWPR QPALCPDLEDCWS*YHRQRDGGASLKKCLL*GR RGVYVLGG*LYRTLPSLCMVDRSGSPGREAGSD DLAPVPGDHHLLHRGLPHLLHGGVGHRLQDEEWY QEE*LPQPDGCAQAGQEHPSAQTGVC*LQCIHE LWGSSGSAITALLQWDSHASRGL*V*ASRRPSL GAASGQTGLRQTPGRGLLWAGGVGRGYRAGQGQT QPCDQSGCEDVEVGRNRERLVRPDLRNGDDEDD REA*EYHQPAGGLHAGWSLVCHRGVCLQGQPAG VPAGPEAPRAGILLQPQPQPRGAALLQGPGVLRL PGGPRHGVSGLQEVHTPRPGSQECPGDRGQCDE DSRLWPRTGHSPHRLL*KDNQRPTACEVDGTRG II*PDLHPPE*CVVFRGAPVGDLHSGRLPIPRCA CGGTFQAAEGGSPHGQAQ*LHQRAVHDDAGLLA CSALTETHLQAAGGRPGPHRGLDLQPGVPGPVH APGPVLPQLSRHPELYVLLRGGFRLLS*AAARGA LPAPTPSPACQWRTQTPLTATHTPSPDSTVSCN PHPQPLLGPPPVRPCPLSCWQEPAAYQGPSCVA CLHPTQLTSPSTSSPPAGERCKEADLCCQPLHPL PDVGPTPLPATRHCLEGREWEPMNRHASESFLS FLLSVWSVLPSPISPSHSGGRCLVLRATAVGRS VLRASIEGDLCPR*VVPVAY*FRY*FALLTKCLV PEDGEAKARLGAVLWPWGPAPNWGLCI*L*RKH KVYKSEYIFTCLFKRVVTRDLPIG*DAPGGWEA SVAIY*KQKRKKRKMFLKRSYIFCYFCCFIFLNY VLNLFSV*VPQ*KLLLL

Sequence FGFR1.bAug05

CCCCGCCGGCGCATAGCGCTCGGAGCGCTCTTGCGGCCACAGGCGCGGCGTCCTCGGCGGCGGGCGGCAGCTAGCGGGAGCCGGGACGCCGGTGCAGCCG CAGCGCGCGGAGGAACCCGGGTGTGCCGGGAGCTGGGCGGCCACGTCCGGACGGGACCGAGACCCCTCGTAGCGCATTGCGGCGACCTCGCCTTCCCCGG CCGCGAGCGCGCCGCTGCTTGAAAAGCCGCGGAACCCAAGGACTTTTCTCCGGTCCGAGCTCGGGGCGCCCCGCAGGGCGCACGGTACCCGTGCTGCAGT CGGGCACGCCGCGGCGCCGGGGCCTCCGCAGGGCGATGGAGCCCGGTCTGCAAGGAAAGTGAGGCGCCGCCGCTGCGTTCTGGAGGAGGGGGGCACAAGG TCTGGAGACCCCGGGTGGCGGACGGGAGCCCTCCCCCCGCCCCGCCTCCGGGGCACCAGCTCCGGCTCCATTGTTCCCGCCCGGGCTGGAGGCGCCGAGC ACCGAGCGCCGCCGGGAGTCGAGCGCCGGCCGCGGAGCTCTTGCGACCCCGCCAGGACCCGAACAGAGCCCGGGGGCGGCGGGCCGGAGCCGGGGACGCG GGCACACGCCCGCTCGCACAAGCCACGGCGGACTCTCCCGAGGCGGAACCTCCACGCCGAGCGAGGGTCAGTTTGAAAAGGAGGATCGAGCTCACTGTGG AGTATCCATGGAGATGTGGAGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGAGCTGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGC CACACTCTGCACCGCTAGGCCGTCCCCGACCTTGCCTGAACAAGCCCAGCCCTGGGGAGCCCCTGTGGAAGTGGAGTCCTTCCTGGTCCACCCCGGTGAC CTGCTGCAGCTTCGCTGTCGGCTGCGGGACGATGTGCAGAGCATCAACTGGCTGCGGGACGGGGTGCAGCTGGCGGAAAGCAACCGCACCCGCATCACAG GGGAGGAGGTGGAGGTGCAGGACTCCGTGCCCGCAGACTCCGGCCTCTATGCTTGCGTAACCAGCAGCCCCTCGGGCAGTGACACCACCTACTTCTCCGT CAATGTTTCAGATGCTCTCCCCTCCTCGGAGGATGATGATGATGATGATGACTCCTCTTCAGAGGAGAAAGAAACAGATAACACCAAACCAAACCCCGTA GCTCCATATTGGACATCCCCAGAAAAGATGGAAAAGAAATTGCATGCAGTGCCGGCTGCCAAGACAGTGAAGTTCAAATGCCCTTCCAGTGGGACCCCAA ACCCCACACTGCGCTGGTTGAAAAATGGCAAAGAATTCAAACCTGACCACAGAATTGGAGGCTACAAGGTCCGTTATGCCACCTGGAGCATCATAATGGA CTCTGTGGTGCCCTCTGACAAGGGCAACTACACCTGCATTGTGGAGAATGAGTACGGCAGCATCAACCACACATACCAGCTGGATGTCGTGGAGCGGTCC CCTCACCGGCCCATCCTGCAAGCAGGGTTGCCCGCCAACAAAACAGTGGCCCTGGGTAGCAACGTGGAGTTCATGTGTAAGGTGTACAGTGACCCGCAGC CGCACATCCAGTGGCTAAAGCACATCGAGGTGAATGGGAGCAAGATTGGCCCAGACAACCTGCCTTATGTCCAGATCTTGAAGACTGCTGGAGTTAATAC CACCGACAAAGAGATGGAGGTGCTTCACTTAAGAAATGTCTCCTTTGAGGACGCAGGGGAGTATACGTGCTTGGCGGGTAACTCTATCGGACTCTCCCAT CACTCTGCATGGTTGACCGTTCTGGAAGCCCTGGAAGAGAGGCCGGCAGTGATGACCTCGCCCCTGTACCTGGAGATCATCATCTATTGCACAGGGGCCT TCCTCATCTCCTGCATGGTGGGGTCGGTCATCGTCTACAAGATGAAGAGTGGTACCAAGAAGAGTGACTTCCACAGCCAGATGGCTGTGCACAAGCTGGC CAAGAGCATCCCTCTGCGCAGACAGGTAACAGTGTCTGCTGACTCCAGTGCATCCATGAACTCTGGGGTTCTTCTGGTTCGGCCATCACGGCTCTCCTCC AGTGGGACTCCCATGCTAGCAGGGGTCTCTGAGTATGAGCTTCCCGAAGACCCTCGCTGGGAGCTGCCTCGGGACAGACTGGTCTTAGGCAAACCCCTGG GAGAGGGCTGCTTTGGGCAGGTGGTGTTGGCAGAGGCTATCGGGCTGGACAAGGACAAACCCAACCGTGTGACCAAAGTGGCTGTGAAGATGTTGAAGTC GGACGCAACAGAGAAAGACTTGTCAGACCTGATCTCAGAAATGGAGATGATGAAGATGATCGGGAAGCATAAGAATATCATCAACCTGCTGGGGGCCTGC ACGCAGGATGGTCCCTTGTATGTCATCGTGGAGTATGCCTCCAAGGGCAACCTGCGGGAGTACCTGCAGGCCCGGAGGCCCCCAGGGCTGGAATACTGCT ACAACCCCAGCCACAACCCAGAGGAGCAGCTCTCCTCCAAGGACCTGGTGTCCTGCGCCTACCAGGTGGCCCGAGGCATGGAGTATCTGGCCTCCAAGAA GTGCATACACCGAGACCTGGCAGCCAGGAATGTCCTGGTGACAGAGGACAATGTGATGAAGATAGCAGACTTTGGCCTCGCACGGGACATTCACCACATC GACTACTATAAAAAGACAACCAACGGCCGACTGCCTGTGAAGTGGATGGCACCCGAGGCATTATTTGACCGGATCTACACCCACCAGAGTGATGTGTGGT CTTTCGGGGTGCTCCTGTGGGAGATCTTCACTCTGGGCGGCTCCCCATACCCCGGTGTGCCTGTGGAGGAACTTTTCAAGCTGCTGAAGGAGGGTCACCG CATGGACAAGCCCAGTAACTGCACCAACGAGCTGTACATGATGATGCGGGACTGCTGGCATGCAGTGCCCTCACAGAGACCCACCTTCAAGCAGCTGGTG GAAGACCTGGACCGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCCCCTGGACCAGTACTCCCCCAGCTTTCCCGACACCCGGA GCTCTACGTGCTCCTCAGGGGAGGATTCCGTCTTCTCTCATGAGCCGCTGCCCGAGGAGCCCTGCCTGCCCCGACACCCAGCCCAGCTTGCCAATGGCGG ACTCAAACGCCGCTGACTGCCACCCACACGCCCTCCCCAGACTCCACCGTCAGCTGTAACCCTCACCCACAGCCCCTGCTGGGCCCACCACCTGTCCGTC CCTGTCCCCTTTCCTGCTGGCAGGAGCCGGCTGCCTACCAGGGGCCTTCCTGTGTGGCCTGCCTTCACCCCACTCAGCTCACCTCTCCCTCCACCTCCTC TCCACCTGCTGGTGAGAGGTGCAAAGAGGCAGATCTTTGCTGCCAGCCACTTCATCCCCTCCCAGATGTTGGACCAACACCCCTCCCTGCCACCAGGCAC TGCCTGGAGGGCAGGGAGTGGGAGCCAATGAACAGGCATGCAAGTGAGAGCTTCCTGAGCTTTCTCCTGTCGGTTTGGTCTGTTTTGCCTTCACCCATAA GCCCCTCGCACTCTGGTGGCAGGTGCCTTGTCCTCAGGGCTACAGCAGTAGGGAGGTCAGTGCTTCGTGCCTCGATTGAAGGTGACCTCTGCCCCAGATA GGTGGTGCCAGTGGCTTATTAATTCCGATACTAGTTTGCTTTGCTGACCAAATGCCTGGTACCAGAGGATGGTGAGGCGAAGGCCAGGTTGGGGGCAGTG TTGTGGCCCTGGGGCCCAGCCCCAAACTGGGGGCTCTGTATATAGCTATGAAGAAAACACAAAGTGTATAAATCTGAGTATATATTTACATGTCTTTTTA AAAGGGTCGTTACCAGAGATTTACCCATCGGGTAAGATGCTCCTGGTGGCTGGGAGGCATCAGTTGCTATATATTAAAAACAAAAAAGAAAAAAAAGGAA AATGTTTTTAAAAAGGTCATATATTTTTTGCTACTTTTGCTGTTTTATTTTTTTAAATTATGTTCTAAACCTATTTTCAGTTTAGGTCCCTCAATAAAAA TTGCTGCTGCTTCATTTATCTATGGGCTGTATGAAAAGGGTGGGAATGTCCACTGGAAAGAAGGGACACCCACGGGCCCTGGGGCTAGGTCTGTCCCGAG GGCACCGCATGCTCCCGGCGCAGGTTCCTTGTAACCTCTTCTTCCTAGGTCCTGCACCCAGACCTCACGACGCACCTCCTGCCTCTCCGCTGCTTTTGGA AAGTCAGAAAAAGAAGATGTCTGCTTCGAGGGCAGGAACCCCATCCATGCAGTAGAGGCGCTGGGCAGAGAGTCAAGGCCCAGCAGCCATCGACCATGGA TGGTTTCCTCCAAGGAAACCGGTGGGGTTGGGCTGGGGAGGGGGCACCTACCTAGGAATAGCCACGGGGTAGAGCTACAGTGATTAAGAGGAAAGCAAGG GCGCGGTTGCTCACGCCTGTAATCCCAGCACTTTGGGACACCGAGGTGGGCAGATCACTTCAGGTCAGGAGTTTGAGACCAGCCTGGCCAACTTAGTGAA ACCCCATCTCTACTAAAAATGCAAAAATTATCCAGGCATGGTGGCACACGCCTGTAATCCCAGCTCCACAGGAGGCTGAGGCAGAATCCCTTGAAGCTGG GAGGCGGAGGTTGCAGTGAGCCGAGATTGCGCCATTGCACTCCAGCCTGGGCAACAGAGAAAACAAAAAGGAAAACAAATGATGAAGGTCTGCAGAAACT GAAACCCAGACATGTGTCTGCCCCCTCTATGTGGGCATGGTTTTGCCAGTGCTTCTAAGTGCAGGAGAACATGTCACCTGAGGCTAGTTTTGCATTCAGG TCCCTGGCTTCGTTTCTTGTTGGTATGCCTCCCCAGATCGTCCTTCCTGTATCCATGTGACCAGACTGTATTTGTTGGGACTGTCGCAGATCTTGGCTTC TTACAGTTCTTCCTGTCCAAACTCCATCCTGTCCCTCAGGAACGGGGGGAAAATTCTCCGAATGTTTTTGGTTTTTTGGCTGCTTGGAATTTACTTCTGC CACCTGCTGGTCATCACTGTCCTCACTAAGTGGATTCTGGCTCCCCCGTACCTCATGGCTCAAACTACCACTCCTCAGTCGCTATATTAAAGCTTATATT TTGCTGGATTACTGCTAAATACAAAAGAAAGTTCAATATGTTTTCATTTCTGTAGGGAAAATGGGATTGCTGCTTTAAATTTCTGAGCTAGGGATTTTTT GGCAGCTGCAGTGTTGGCGACTATTGTAAAATTCTCTTTGTTTCTCTCTGTAAATAGCACCTGCTAACATTACAATTTGTATTTATGTTTAAAGAAGGCA TCATTTGGTGAACAGAACTAGGAAATGAATTGTATTTTTAGTAGAGACGGTGTTTCACCATGTTGGCCAAGCTGGTCTTGAACTCCTGACCTCGGGTGAT CCACCCACCTCAGCCTCCCAAAGTGCTGGATTACAGGCATGAGCCACTGCTACTGGCCTCAGTTATTTTCTAATAG

FGFR1.F11 (forward primer)
FGFR1.R11 (reverse primer)
Single exon skipping

Frame 0

PRRRIALGALLRPQARRPRRRAAASGSRDAGAA AARGGTRVCRELGGHVRTGPRPLVAHCGDLAFPG RERAAA*KAAEPKDFSPVRARGAPQGARYPCCS RARRGAGASAGRWSPVCKESEAPPLRSGGGGHK VWRPRVADGSPPPAPPPGHQLRLHCSRPGWRRRA PSAAGSRAPAAELLRPRQDPNRARGRRAGAGDA GTRPLAQATADSPEAEPPRRARVSLKRRIELTV EYPWRCGALSPTSNCRTGMWSWKCLLFWAVLVTA TLCTARPSPTLPEQAQPWGAPVEVESFLVHPGD LLQLRCRLRDDVQSINWLRDGVQLAESNRTRIT GEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSV NVSDALPSSEDDDDDDDSSSEEKETDNTKPNPV APYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTP NPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMD SVVPSDKGNYTCIVENEYGSINHTYQLDVVERS PHRPILQAGLPANKTVALGSNVEFMCKVYSDPQ PHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNT TDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSH HSAWLTVLEALEERPAVMTSPLYLEIIIYCTGA FLISCMVGSVIVYKMKSGTKKSDFHSQMAVHKLA KSIPLRRQVTVSADSSASMNSGVLLVRPSRLSS SGTPMLAGVSEYELPEDPRWELPRDRLVLGKPL GEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKS DATEKDLSDLISEMEMMKMIGKHKNIINLLGAC TQDGPLYVIVEYASKGNLREYLQARRPPGLEYC YNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKK CIHRDLAARNVLVTEDNVMKIADFGLARDIHHI DYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVW SFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHR MDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLV EDLDRIVALTSNQEYLDLSMPLDQYSPSFPDTR SSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGG LKRR*LPPTRPPQTPPSAVTLTHSPCWAHHLSV PVPFPAGRSRLPTRGLPVWPAFTPLSSPLPPPP LHLLVRGAKRQIFAASHFIPSQMLDQHPSLPPGT AWRAGSGSQ*TGMQVRAS*AFSCRFGLFCLHP* APRTLVAGALSSGLQQ*GGQCFVPRLKVTSAPD RWCQWLINSDTSLLC*PNAWYQRMVRRRPGWGQC CGPGAQPQTGGSVYSYEENTKCINLSIYLHVFL KGSLPEIYPSGKMLLVAGRHQLLYIKNKKEKKG KCF*KGHIFFATFAVLFF*IMF*TYFQFRSLNKN CCCFIYLWAV*KGWECPLERRDTHGPWG*VCPE GTACSRRRFLVTSSS*VLHPDLTTHLLPLRCFW KVRKRRCLLRGQEPHPCSRGAGQRVKAQQPSTMD GFLQGNRWGWAGEGAPT*E*PRGRATVIKRKAR ARLLTPVIPALWDTEVGRSLQVRSLRPAWPT** NPISTKNAKIIQAWWHTPVIPAPQEAEAESLEAG RRRLQ*AEIAPLHSSLGNRENKKENK**RSAET ETQTCVCPLYVGMVLPVLLSAGEHVT*G*FCIQ VPGFVSCWYASPDRPSCIHVTRLYLLGLSQILAS YSSSCPNSILSLRNGGKILRMFLVFWLLGIYFC HLLVITVLTKWILAPPYLMAQTTTPQSLY*SLY FAGLLLNTKESSICFHFCRENGIAALNF*ARDFL AAAVLATIVKFSLFLSVNSTC*HYNLYLCLKKA SFGEQN*EMNCIFSRDGVSPCWPSWS*TPDLG* STHLSLPKCWITGMSHCYWPQLFSN

Frame 1

PAGA*RSERSCGHRRGVLGGGRQLAGAGTPVQP QRAEEPGCAGSWAATSGRDRDPS*RIAATSPSP AASAPLLEKPRNPRTFLRSELGAPRRAHGTRAAV GHAAAPGPPQGDGARSARKVRRRRCVLEEGGTR SGDPGWRTGALPPPRLRGTSSGSIVPARAGGAE HRAPPGVERRPRSSCDPARTRTEPGGGGPEPGTR AHARSHKPRRTLPRRNLHAERGSV*KGGSSSLW SIHGDVEPCHQPLTAELGCGAGSASSSGLCWSQ PHSAPLGRPRPCLNKPSPGEPLWKWSPSWSTPVT CCSFAVGCGTMCRASTGCGTGCSWRKATAPASQ GRRWRCRTPCPQTPASMLA*PAAPRAVTPPTSP SMFQMLSPPRRMMMMMMTPLQRRKKQITPNQTP* LHIGHPQKRWKRNCMQCRLPRQ*SSNALPVGPQ TPHCAG*KMAKNSNLTTELEATRSVMPPGAS*W TLWCPLTRATTPALWRMSTAASTTHTSWMSWSGP LTGPSCKQGCPPTKQWPWVATWSSCVRCTVTRS RTSSG*STSR*MGARLAQTTCLMSRS*RLLELI PPTKRWRCFT*EMSPLRTQGSIRAWRVTLSDSPI TLHG*PFWKPWKRGRQ**PRPCTWRSSSIAQGP SSSPAWWGRSSSTR*RVVPRRVTSTARWLCTSW PRASLCADR*QCLLTPVHP*TLGFFWFGHHGSPP VGLPC*QGSLSMSFPKTLAGSCLGTDWS*ANPW ERAALGRWCWQRLSGWTRTNPTV*PKWL*RC*S RTQQRKTCQT*SQKWR**R*SGSIRISSTCWGPA RRMVPCMSSWSMPPRATCGSTCRPGGPQGWNTA TTPATTQRSSSPPRTWCPAPTRWPEAWSIWPPR SAYTETWQPGMSW*QRTM**R*QTLASHGTFTTS TTIKRQPTADCL*SGWHPRHYLTGSTPTRVMCG LSGCSCGRSSLWAAPHTPVCLWRNFSSC*RRVT AWTSPVTAPTSCT**CGTAGMQCPHRDPPSSSWW KTWTASWP*PPTRSTWTCPCPWTSTPPAFPTPG ALRAPQGRIPSSLMSRCPRSPACPDTQPSLPMA DSNAADCHPHALPRLHRQL*PSPTAPAGPTTCPS LSPFLLAGAGCLPGAFLCGLPSPHSAHLSLHLL STCW*EVQRGRSLLPATSSPPRCWTNTPPCHQA LPGGQGVGANEQACK*ELPELSPVGLVCFAFTHK PLALWWQVPCPQGYSSREVSASCLD*R*PLPQI GGASGLLIPILVCFADQMPGTRGW*GEGQVGGS VVALGPSPKLGALYIAMKKTQSV*I*VYIYMSF* KGRYQRFTHRVRCSWWLGGISCYILKTKKKKKE NVFKKVIYFLLLLLFYFFKLCSKPIFSLGPSIK IAAASFIYGLYEKGGNVHWKEGTPTGPGARSVPR APHAPGAGSL*PLLPRSCTQTSRRTSCLSAAFG KSEKEDVCFEGRNPIHAVEALGRESRPSSHRPW MVSSKETGGVGLGRGHLPRNSHGVELQ*LRGKQG RGCSRL*SQHFGTPRWADHFRSGV*DQPGQLSE TPSLLKMQKLSRHGGTRL*SQLHRRLRQNPLKL GGGGCSEPRLRHCTPAWATEKTKRKTNDEGLQKL KPRHVSAPSMWAWFCQCF*VQENMSPEASFAFR SLASFLVGMPPQIVLPVSM*PDCICWDCRRSWL LTVLPVQTPSCPSGTGGKFSECFWFFGCLEFTSA TCWSSLSSLSGFWLPRTSWLKLPLLSRYIKAYI LLDYC*IQKKVQYVFISVGKMGLLL*ISELGIF WQLQCWRLL*NSLCFSL*IAPANITICIYV*RRH HLVNRTRK*IVFLVETVFHHVGQAGLELLTSGD PPTSASQSAGLQA*ATATGLSYFLI

Frame 2

PPAHSARSALAATGAASSAAGGS*REPGRRCSR SARRNPGVPGAGRPRPDGTETPRSALRRPRLPR PRARRCLKSRGTQGLFSGPSSGRPAGRTVPVLQ SGTPRRRGLRRAMEPGLQGK*GAAAAFWRRGAQG LETPGGGREPSPRPASGAPAPAPLFPPGLEAPS TERRRESSAGRGALATPPGPEQSPGAAGRSRGR GHTPARTSHGGLSRGGTSTPSEGQFEKEDRAHCG VSMEMWSLVTNL*LQNWDVELEVPPLLGCAGHS HTLHR*AVPDLA*TSPALGSPCGSGVLPGPPR* PAAASLSAAGRCAEHQLAAGRGAAGGKQPHPHHR GGGGGAGLRARRLRPLCLRNQQPLGQ*HHLLLR QCFRCSPLLGG******LLFRGERNR*HQTKPR SSILDIPRKDGKEIACSAGCQDSEVQMPFQWDPK PHTALVEKWQRIQT*PQNWRLQGPLCHLEHHNG LCGAL*QGQLHLHCGE*VRQHQPHIPAGCRGAV PSPAHPASRVARQQNSGPG*QRGVHV*GVQ*PAA AHPVAKAHRGEWEQDWPRQPALCPDLEDCWS*Y HRQRDGGASLKKCLL*GRRGVYVLGG*LYRTLP SLCMVDRSGSPGREAGSDDLAPVPGDHHLLHRGL PHLLHGGVGHRLQDEEWYQEE*LPQPDGCAQAG QEHPSAQTGNSVC*LQCIHELWGSSGSAITALL QWDSHASRGL*V*ASRRPSLGAASGQTGLRQTPG RGLLWAGGVGRGYRAGQGQTQPCDQSGCEDVEV GRNRERLVRPDLRNGDDEDDREA*EYHQPAGGL HAGWSLVCHRGVCLQGQPAGVPAGPEAPRAGILL QPQPQPRGAALLQGPGVLRLPGGPRHGVSGLQE VHTPRPGSQECPGDRGQCDEDSRLWPRTGHSPH RLL*KDNQRPTACEVDGTRGII*PDLHPPE*CVV FRGAPVGDLHSGRLPIPRCACGGTFQAAEGGSP HGQAQ*LHQRAVHDDAGLLACSALTETHLQAAG GRPGPHRGLDLQPGVPGPVHAPGPVLPQLSRHPE LYVLLRGGFRLLS*AAARGALPAPTPSPACQWR TQTPLTATHTPSPDSTVSCNPHPQPLLGPPPVR PCPLSCWQEPAAYQGPSCVACLHPTQLTSPSTSS PPAGERCKEADLCCQPLHPLPDVGPTPLPATRH CLEGREWEPMNRHASESFLSFLLSVWSVLPSPI SPSHSGGRCLVLRATAVGRSVLRASIEGDLCPR* VVPVAY*FRY*FALLTKCLVPEDGEAKARLGAV LWPWGPAPNWGLCI*L*RKHKVYKSEYIFTCLF KRVVTRDLPIG*DAPGGWEASVAIY*KQKRKKRK MFLKRSYIFCYFCCFIFLNYVLNLFSV*VPQ*K LLLLHLSMGCMKRVGMSTGKKGHPRALGLGLSR GHRMLPAQVPCNLFFLGPAPRPHDAPPASPLLLE SQKKKMSASRAGTPSMQ*RRWAESQGPAAIDHG WFPPRKPVGLGWGGGTYLGIATG*SYSD*EESK GAVAHACNPSTLGHRGGQITSGQEFETSLANLVK PHLY*KCKNYPGMVAHACNPSSTGG*GRIP*SW EAEVAVSRDCAIALQPGQQRKQKGKQMMKVCRN *NPDMCLPPLCGHGFASASKCRRTCHLRLVLHSG PWLRFLLVCLPRSSFLYPCDQTVFVGTVADLGF LQFFLSKLHPVPQERGENSPNVFGFLAAWNLLL PPAGHHCPH*VDSGSPVPHGSNYHSSVAILKLIF CWITAKYKRKFNMFSFL*GKWDCCFKFLS*GFF GSCSVGDYCKILFVSLCK*HLLTLQFVFMFKEG IIW*TELGNELYF**RRCFTMLAKLVLNS*PRVI HPPQPPKVLDYRHEPLLLASVIF**

Sequence FGFR1.aAug05

CCCCGCCGGCGCATAGCGCTCGGAGCGCTCTTGCGGCCACAGGCGCGGCGTCCTCGGCGGCGGGCGGCAGCTAGCGGGAGCCGGGACGCCGGTGCAGCCG CAGCGCGCGGAGGAACCCGGGTGTGCCGGGAGCTGGGCGGCCACGTCCGGACGGGACCGAGACCCCTCGTAGCGCATTGCGGCGACCTCGCCTTCCCCGG CCGCGAGCGCGCCGCTGCTTGAAAAGCCGCGGAACCCAAGGACTTTTCTCCGGTCCGAGCTCGGGGCGCCCCGCAGGGCGCACGGTACCCGTGCTGCAGT CGGGCACGCCGCGGCGCCGGGGCCTCCGCAGGGCGATGGAGCCCGGTCTGCAAGGAAAGTGAGGCGCCGCCGCTGCGTTCTGGAGGAGGGGGGCACAAGG TCTGGAGACCCCGGGTGGCGGACGGGAGCCCTCCCCCCGCCCCGCCTCCGGGGCACCAGCTCCGGCTCCATTGTTCCCGCCCGGGCTGGAGGCGCCGAGC ACCGAGCGCCGCCGGGAGTCGAGCGCCGGCCGCGGAGCTCTTGCGACCCCGCCAGGACCCGAACAGAGCCCGGGGGCGGCGGGCCGGAGCCGGGGACGCG GGCACACGCCCGCTCGCACAAGCCACGGCGGACTCTCCCGAGGCGGAACCTCCACGCCGAGCGAGGGTCAGTTTGAAAAGGAGGATCGAGCTCACTGTGG AGTATCCATGGAGATGTGGAGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGAGCTGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGC CACACTCTGCACCGCTAGGCCGTCCCCGACCTTGCCTGAACAAGCCCAGCCCTGGGGAGCCCCTGTGGAAGTGGAGTCCTTCCTGGTCCACCCCGGTGAC CTGCTGCAGCTTCGCTGTCGGCTGCGGGACGATGTGCAGAGCATCAACTGGCTGCGGGACGGGGTGCAGCTGGCGGAAAGCAACCGCACCCGCATCACAG GGGAGGAGGTGGAGGTGCAGGACTCCGTGCCCGCAGACTCCGGCCTCTATGCTTGCGTAACCAGCAGCCCCTCGGGCAGTGACACCACCTACTTCTCCGT CAATGTTTCAGATGCTCTCCCCTCCTCGGAGGATGATGATGATGATGATGACTCCTCTTCAGAGGAGAAAGAAACAGATAACACCAAACCAAACCGTATG CCCGTAGCTCCATATTGGACATCCCCAGAAAAGATGGAAAAGAAATTGCATGCAGTGCCGGCTGCCAAGACAGTGAAGTTCAAATGCCCTTCCAGTGGGA CCCCAAACCCCACACTGCGCTGGTTGAAAAATGGCAAAGAATTCAAACCTGACCACAGAATTGGAGGCTACAAGGTCCGTTATGCCACCTGGAGCATCAT AATGGACTCTGTGGTGCCCTCTGACAAGGGCAACTACACCTGCATTGTGGAGAATGAGTACGGCAGCATCAACCACACATACCAGCTGGATGTCGTGGAG CGGTCCCCTCACCGGCCCATCCTGCAAGCAGGGTTGCCCGCCAACAAAACAGTGGCCCTGGGTAGCAACGTGGAGTTCATGTGTAAGGTGTACAGTGACC CGCAGCCGCACATCCAGTGGCTAAAGCACATCGAGGTGAATGGGAGCAAGATTGGCCCAGACAACCTGCCTTATGTCCAGATCTTGAAGACTGCTGGAGT TAATACCACCGACAAAGAGATGGAGGTGCTTCACTTAAGAAATGTCTCCTTTGAGGACGCAGGGGAGTATACGTGCTTGGCGGGTAACTCTATCGGACTC TCCCATCACTCTGCATGGTTGACCGTTCTGGAAGCCCTGGAAGAGAGGCCGGCAGTGATGACCTCGCCCCTGTACCTGGAGATCATCATCTATTGCACAG GGGCCTTCCTCATCTCCTGCATGGTGGGGTCGGTCATCGTCTACAAGATGAAGAGTGGTACCAAGAAGAGTGACTTCCACAGCCAGATGGCTGTGCACAA GCTGGCCAAGAGCATCCCTCTGCGCAGACAGGTAACAGTGTCTGCTGACTCCAGTGCATCCATGAACTCTGGGGTTCTTCTGGTTCGGCCATCACGGCTC TCCTCCAGTGGGACTCCCATGCTAGCAGGGGTCTCTGAGTATGAGCTTCCCGAAGACCCTCGCTGGGAGCTGCCTCGGGACAGACTGGTCTTAGGCAAAC CCCTGGGAGAGGGCTGCTTTGGGCAGGTGGTGTTGGCAGAGGCTATCGGGCTGGACAAGGACAAACCCAACCGTGTGACCAAAGTGGCTGTGAAGATGTT GAAGTCGGACGCAACAGAGAAAGACTTGTCAGACCTGATCTCAGAAATGGAGATGATGAAGATGATCGGGAAGCATAAGAATATCATCAACCTGCTGGGG GCCTGCACGCAGGATGGTCCCTTGTATGTCATCGTGGAGTATGCCTCCAAGGGCAACCTGCGGGAGTACCTGCAGGCCCGGAGGCCCCCAGGGCTGGAAT ACTGCTACAACCCCAGCCACAACCCAGAGGAGCAGCTCTCCTCCAAGGACCTGGTGTCCTGCGCCTACCAGGTGGCCCGAGGCATGGAGTATCTGGCCTC CAAGAAGTGCATACACCGAGACCTGGCAGCCAGGAATGTCCTGGTGACAGAGGACAATGTGATGAAGATAGCAGACTTTGGCCTCGCACGGGACATTCAC CACATCGACTACTATAAAAAGACAACCAACGGCCGACTGCCTGTGAAGTGGATGGCACCCGAGGCATTATTTGACCGGATCTACACCCACCAGAGTGATG TGTGGTCTTTCGGGGTGCTCCTGTGGGAGATCTTCACTCTGGGCGGCTCCCCATACCCCGGTGTGCCTGTGGAGGAACTTTTCAAGCTGCTGAAGGAGGG TCACCGCATGGACAAGCCCAGTAACTGCACCAACGAGCTGTACATGATGATGCGGGACTGCTGGCATGCAGTGCCCTCACAGAGACCCACCTTCAAGCAG CTGGTGGAAGACCTGGACCGCATCGTGGCCTTGACCTCCAACCAGGAGTACCTGGACCTGTCCATGCCCCTGGACCAGTACTCCCCCAGCTTTCCCGACA CCCGGAGCTCTACGTGCTCCTCAGGGGAGGATTCCGTCTTCTCTCATGAGCCGCTGCCCGAGGAGCCCTGCCTGCCCCGACACCCAGCCCAGCTTGCCAA TGGCGGACTCAAACGCCGCTGACTGCCACCCACACGCCCTCCCCAGACTCCACCGTCAGCTGTAACCCTCACCCACAGCCCCTGCTGGGCCCACCACCTG TCCGTCCCTGTCCCCTTTCCTGCTGGCAGGAGCCGGCTGCCTACCAGGGGCCTTCCTGTGTGGCCTGCCTTCACCCCACTCAGCTCACCTCTCCCTCCAC CTCCTCTCCACCTGCTGGTGAGAGGTGCAAAGAGGCAGATCTTTGCTGCCAGCCACTTCATCCCCTCCCAGATGTTGGACCAACACCCCTCCCTGCCACC AGGCACTGCCTGGAGGGCAGGGAGTGGGAGCCAATGAACAGGCATGCAAGTGAGAGCTTCCTGAGCTTTCTCCTGTCGGTTTGGTCTGTTTTGCCTTCAC CCATAAGCCCCTCGCACTCTGGTGGCAGGTGCCTTGTCCTCAGGGCTACAGCAGTAGGGAGGTCAGTGCTTCGTGCCTCGATTGAAGGTGACCTCTGCCC CAGATAGGTGGTGCCAGTGGCTTATTAATTCCGATACTAGTTTGCTTTGCTGACCAAATGCCTGGTACCAGAGGATGGTGAGGCGAAGGCCAGGTTGGGG GCAGTGTTGTGGCCCTGGGGCCCAGCCCCAAACTGGGGGCTCTGTATATAGCTATGAAGAAAACACAAAGTGTATAAATCTGAGTATATATTTACATGTC TTTTTAAAAGGGTCGTTACCAGAGATTTACCCATCGGGTAAGATGCTCCTGGTGGCTGGGAGGCATCAGTTGCTATATATTAAAAACAAAAAAGAAAAAA AAGGAAAATGTTTTTAAAAAGGTCATATATTTTTTGCTACTTTTGCTGTTTTATTTTTTTAAATTATGTTCTAAACCTATTTTCAGTTTAGGTCCCTCAA TAAAAATTGCTGCTGCTTC

FGFR1.F11 (forward primer)
FGFR1.R11 (reverse primer)
Single exon skipping

Frame 0

PRRRIALGALLRPQARRPRRRAAASGSRDAGAA AARGGTRVCRELGGHVRTGPRPLVAHCGDLAFPG RERAAA*KAAEPKDFSPVRARGAPQGARYPCCS RARRGAGASAGRWSPVCKESEAPPLRSGGGGHK VWRPRVADGSPPPAPPPGHQLRLHCSRPGWRRRA PSAAGSRAPAAELLRPRQDPNRARGRRAGAGDA GTRPLAQATADSPEAEPPRRARVSLKRRIELTV EYPWRCGALSPTSNCRTGMWSWKCLLFWAVLVTA TLCTARPSPTLPEQAQPWGAPVEVESFLVHPGD LLQLRCRLRDDVQSINWLRDGVQLAESNRTRIT GEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSV NVSDALPSSEDDDDDDDSSSEEKETDNTKPNRM PVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSG TPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSII MDSVVPSDKGNYTCIVENEYGSINHTYQLDVVE RSPHRPILQAGLPANKTVALGSNVEFMCKVYSD PQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGV NTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGL SHHSAWLTVLEALEERPAVMTSPLYLEIIIYCT GAFLISCMVGSVIVYKMKSGTKKSDFHSQMAVHK LAKSIPLRRQVTVSADSSASMNSGVLLVRPSRL SSSGTPMLAGVSEYELPEDPRWELPRDRLVLGK PLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKML KSDATEKDLSDLISEMEMMKMIGKHKNIINLLG ACTQDGPLYVIVEYASKGNLREYLQARRPPGLE YCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLAS KKCIHRDLAARNVLVTEDNVMKIADFGLARDIH HIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSD VWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEG HRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQ LVEDLDRIVALTSNQEYLDLSMPLDQYSPSFPD TRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLAN GGLKRR*LPPTRPPQTPPSAVTLTHSPCWAHHL SVPVPFPAGRSRLPTRGLPVWPAFTPLSSPLPP PPLHLLVRGAKRQIFAASHFIPSQMLDQHPSLPP GTAWRAGSGSQ*TGMQVRAS*AFSCRFGLFCLH P*APRTLVAGALSSGLQQ*GGQCFVPRLKVTSA PDRWCQWLINSDTSLLC*PNAWYQRMVRRRPGWG QCCGPGAQPQTGGSVYSYEENTKCINLSIYLHV FLKGSLPEIYPSGKMLLVAGRHQLLYIKNKKEK KGKCF*KGHIFFATFAVLFF*IMF*TYFQFRSLN KNCCCF

Frame 1

PAGA*RSERSCGHRRGVLGGGRQLAGAGTPVQP QRAEEPGCAGSWAATSGRDRDPS*RIAATSPSP AASAPLLEKPRNPRTFLRSELGAPRRAHGTRAAV GHAAAPGPPQGDGARSARKVRRRRCVLEEGGTR SGDPGWRTGALPPPRLRGTSSGSIVPARAGGAE HRAPPGVERRPRSSCDPARTRTEPGGGGPEPGTR AHARSHKPRRTLPRRNLHAERGSV*KGGSSSLW SIHGDVEPCHQPLTAELGCGAGSASSSGLCWSQ PHSAPLGRPRPCLNKPSPGEPLWKWSPSWSTPVT CCSFAVGCGTMCRASTGCGTGCSWRKATAPASQ GRRWRCRTPCPQTPASMLA*PAAPRAVTPPTSP SMFQMLSPPRRMMMMMMTPLQRRKKQITPNQTVC P*LHIGHPQKRWKRNCMQCRLPRQ*SSNALPVG PQTPHCAG*KMAKNSNLTTELEATRSVMPPGAS *WTLWCPLTRATTPALWRMSTAASTTHTSWMSWS GPLTGPSCKQGCPPTKQWPWVATWSSCVRCTVT RSRTSSG*STSR*MGARLAQTTCLMSRS*RLLE LIPPTKRWRCFT*EMSPLRTQGSIRAWRVTLSDS PITLHG*PFWKPWKRGRQ**PRPCTWRSSSIAQ GPSSSPAWWGRSSSTR*RVVPRRVTSTARWLCT SWPRASLCADR*QCLLTPVHP*TLGFFWFGHHGS PPVGLPC*QGSLSMSFPKTLAGSCLGTDWS*AN PWERAALGRWCWQRLSGWTRTNPTV*PKWL*RC *SRTQQRKTCQT*SQKWR**R*SGSIRISSTCWG PARRMVPCMSSWSMPPRATCGSTCRPGGPQGWN TATTPATTQRSSSPPRTWCPAPTRWPEAWSIWP PRSAYTETWQPGMSW*QRTM**R*QTLASHGTFT TSTTIKRQPTADCL*SGWHPRHYLTGSTPTRVM CGLSGCSCGRSSLWAAPHTPVCLWRNFSSC*RR VTAWTSPVTAPTSCT**CGTAGMQCPHRDPPSSS WWKTWTASWP*PPTRSTWTCPCPWTSTPPAFPT PGALRAPQGRIPSSLMSRCPRSPACPDTQPSLP MADSNAADCHPHALPRLHRQL*PSPTAPAGPTTC PSLSPFLLAGAGCLPGAFLCGLPSPHSAHLSLH LLSTCW*EVQRGRSLLPATSSPPRCWTNTPPCH QALPGGQGVGANEQACK*ELPELSPVGLVCFAFT HKPLALWWQVPCPQGYSSREVSASCLD*R*PLP QIGGASGLLIPILVCFADQMPGTRGW*GEGQVG GSVVALGPSPKLGALYIAMKKTQSV*I*VYIYMS F*KGRYQRFTHRVRCSWWLGGISCYILKTKKKK KENVFKKVIYFLLLLLFYFFKLCSKPIFSLGPS IKIAAA

Frame 2

PPAHSARSALAATGAASSAAGGS*REPGRRCSR SARRNPGVPGAGRPRPDGTETPRSALRRPRLPR PRARRCLKSRGTQGLFSGPSSGRPAGRTVPVLQ SGTPRRRGLRRAMEPGLQGK*GAAAAFWRRGAQG LETPGGGREPSPRPASGAPAPAPLFPPGLEAPS TERRRESSAGRGALATPPGPEQSPGAAGRSRGR GHTPARTSHGGLSRGGTSTPSEGQFEKEDRAHCG VSMEMWSLVTNL*LQNWDVELEVPPLLGCAGHS HTLHR*AVPDLA*TSPALGSPCGSGVLPGPPR* PAAASLSAAGRCAEHQLAAGRGAAGGKQPHPHHR GGGGGAGLRARRLRPLCLRNQQPLGQ*HHLLLR QCFRCSPLLGG******LLFRGERNR*HQTKPY ARSSILDIPRKDGKEIACSAGCQDSEVQMPFQWD PKPHTALVEKWQRIQT*PQNWRLQGPLCHLEHH NGLCGAL*QGQLHLHCGE*VRQHQPHIPAGCRG AVPSPAHPASRVARQQNSGPG*QRGVHV*GVQ*P AAAHPVAKAHRGEWEQDWPRQPALCPDLEDCWS *YHRQRDGGASLKKCLL*GRRGVYVLGG*LYRT LPSLCMVDRSGSPGREAGSDDLAPVPGDHHLLHR GLPHLLHGGVGHRLQDEEWYQEE*LPQPDGCAQ AGQEHPSAQTGNSVC*LQCIHELWGSSGSAITA LLQWDSHASRGL*V*ASRRPSLGAASGQTGLRQT PGRGLLWAGGVGRGYRAGQGQTQPCDQSGCEDV EVGRNRERLVRPDLRNGDDEDDREA*EYHQPAG GLHAGWSLVCHRGVCLQGQPAGVPAGPEAPRAGI LLQPQPQPRGAALLQGPGVLRLPGGPRHGVSGL QEVHTPRPGSQECPGDRGQCDEDSRLWPRTGHS PHRLL*KDNQRPTACEVDGTRGII*PDLHPPE*C VVFRGAPVGDLHSGRLPIPRCACGGTFQAAEGG SPHGQAQ*LHQRAVHDDAGLLACSALTETHLQA AGGRPGPHRGLDLQPGVPGPVHAPGPVLPQLSRH PELYVLLRGGFRLLS*AAARGALPAPTPSPACQ WRTQTPLTATHTPSPDSTVSCNPHPQPLLGPPP VRPCPLSCWQEPAAYQGPSCVACLHPTQLTSPST SSPPAGERCKEADLCCQPLHPLPDVGPTPLPAT RHCLEGREWEPMNRHASESFLSFLLSVWSVLPS PISPSHSGGRCLVLRATAVGRSVLRASIEGDLCP R*VVPVAY*FRY*FALLTKCLVPEDGEAKARLG AVLWPWGPAPNWGLCI*L*RKHKVYKSEYIFTC LFKRVVTRDLPIG*DAPGGWEASVAIY*KQKRKK RKMFLKRSYIFCYFCCFIFLNYVLNLFSV*VPQ *KLLLL

Sequence FGFR1.gAug05

CCCCGCCGGCGCATAGCGCTCGGAGCGCTCTTGCGGCCACAGGCGCGGCGTCCTCGGCGGCGGGCGGCAGCTAGCGGGAGCCGGGACGCCGGTGCAGCCG CAGCGCGCGGAGGAACCCGGGTGTGCCGGGAGCTGGGCGGCCACGTCCGGACGGGACCGAGACCCCTCGTAGCGCATTGCGGCGACCTCGCCTTCCCCGG CCGCGAGCGCGCCGCTGCTTGAAAAGCCGCGGAACCCAAGGACTTTTCTCCGGTCCGAGCTCGGGGCGCCCCGCAGGGCGCACGGTACCCGTGCTGCAGT CGGGCACGCCGCGGCGCCGGGGCCTCCGCAGGGCGATGGAGCCCGGTCTGCAAGGAAAGTGAGGCGCCGCCGCTGCGTTCTGGAGGAGGGGGGCACAAGG TCTGGAGACCCCGGGTGGCGGACGGGAGCCCTCCCCCCGCCCCGCCTCCGGGGCACCAGCTCCGGCTCCATTGTTCCCGCCCGGGCTGGAGGCGCCGAGC ACCGAGCGCCGCCGGGAGTCGAGCGCCGGCCGCGGAGCTCTTGCGACCCCGCCAGGACCCGAACAGAGCCCGGGGGCGGCGGGCCGGAGCCGGGGACGCG GGCACACGCCCGCTCGCACAAGCCACGGCGGACTCTCCCGAGGCGGAACCTCCACGCCGAGCGAGGGTCAGTTTGAAAAGGAGGATCGAGCTCACTGTGG AGTATCCATGGAGATGTGGAGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGAGCTGGAAGTGCCTCCTCTTCTGGGCTGTGCTGGTCACAGC CACACTCTGCACCGCTAGGCCGTCCCCGACCTTGCCTGAACAAGGATGGCAGCTGTGACCCGGGATTTCGGTGAGATGCTTCTGCACTCTGGCCGGGTCC TGCCAGCCGAAGCCCAGCCCTGGGGAGCCCCTGTGGAAGTGGAGTCCTTCCTGGTCCACCCCGGTGACCTGCTGCAGCTTCGCTGTCGGCTGCGGGACGA TGTGCAGAGCATCAACTGGCTGCGGGACGGGGTGCAGCTGGCGGAAAGCAACCGCACCCGCATCACAGGGGAGGAGGTGGAGGTGCAGGACTCCGTGCCC GCAGACTCCGGCCTCTATGCTTGCGTAACCAGCAGCCCCTCGGGCAGTGACACCACCTACTTCTCCGTCAATGTTTCAGATGCTCTCCCCTCCTCGGAGG ATGATGATGATGATGATGACTCCTCTTCAGAGGAGAAAGAAACAGATAACACCAAACCAAACCGTATGCCCGTAGCTCCATATTGGACATCCCCAGAAAA GATGGAAAAGAAATTGCATGCAGTGCCGGCTGCCAAGACAGTGAAGTTCAAATGCCCTTCCAGTGGGACCCCAAACCCCACACTGCGCTGGTTGAAAAAT GGCAAAGAATTCAAACCTGACCACAGAATTGGAGGCTACAAGGTCCGTTATGCCACCTGGAGCATCATAATGGACTCTGTGGTGCCCTCTGACAAGGGCA ACTACACCTGCATTGTGGAGAATGAGTACGGCAGCATCAACCACACATACCAGCTGGATGTCGTGGAGCGGTCCCCTCACCGGCCCATCCTGCAAGCAGG GTTGCCCGCCAACAAAACAGTGGCCCTGGGTAGCAACGTGGAGTTCATGTGTAAGGTGTACAGTGACCCGCAGCCGCACATCCAGTGGCTAAAGCACATC GAGGTGAATGGGAGCAAGATTGGCCCAGACAACCTGCCTTATGTCCAGATCTTGAAGACTGCTGGAGTTAATACCACCGACAAAGAGATGGAGGTGCTTC ACTTAAGAAATGTCTCCTTTGAGGACGCAGGGGAGTATACGTGCTTGGCGGGTAACTCTATCGGACTCTCCCATCACTCTGCATGGTTGACCGTTCTGGA AGCCCTGGAAGAGAGGCCGGCAGTGATGACCTCGCCCCTGTACCTGGAGATCATCATCTATTGCACAGGGGCCTTCCTCATCTCCTGCATGGTGGGGTCG GTCATCGTCTACAAGATGAAGAGTGGTACCAAGAAGAGTGACTTCCACAGCCAGATGGCTGTGCACAAGCTGGCCAAGAGCATCCCTCTGCGCAGACAGG TGTCTGCTGACTCCAGTGCATCCATGAACTCTGGGGTTCTTCTGGTTCGGCCATCACGGCTCTCCTCCAGTGGGACTCCCATGCTAGCAGGGGTCTCTGA GTATGAGCTTCCCGAAGACCCTCGCTGGGAGCTGCCTCGGGACAGACTGGTCTTAGGCAAACCCCTGGGAGAGGGCTGCTTTGGGCAGGTGGTGTTGGCA GAGGCTATCGGGCTGGACAAGGACAAACCCAACCGTGTGACCAAAGTGGCTGTGAAGATGTTGAAGTCGGACGCAACAGAGAAAGACTTGTCAGACCTGA TCTCAGAAATGGAGATGATGAAGATGATCGGGAAGCATAAGAATATCATCAACCTGCTGGGGGCCTGCACGCAGGATGGTCCCTTGTATGTCATCGTGGA GTATGCCTCCAAGGGCAACCTGCGGGAGTACCTGCAGGCCCGGAGGCCCCCAGGGCTGGAATACTGCTACAACCCCAGCCACAACCCAGAGGAGCAGCTC TCCTCCAAGGACCTGGTGTCCTGCGCCTACCAGGTGGCCCGAGGCATGGAGTATCTGGCCTCCAAGAAGTGCATACACCGAGACCTGGCAGCCAGGAATG TCCTGGTGACAGAGGACAATGTGATGAAGATAGCAGACTTTGGCCTCGCACGGGACATTCACCACATCGACTACTATAAAAAGACAACCAACGGCCGACT GCCTGTGAAGTGGATGGCACCCGAGGCATTATTTGACCGGATCTACACCCACCAGAGTGATGTGTGGTCTTTCGGGGTGCTCCTGTGGGAGATCTTCACT CTGGGCGGCTCCCCATACCCCGGTGTGCCTGTGGAGGAACTTTTCAAGCTGCTGAAGGAGGGTCACCGCATGGACAAGCCCAGTAACTGCACCAACGAGC TGTACATGATGATGCGGGACTGCTGGCATGCAGTGCCCTCACAGAGACCCACCTTCAAGCAGCTGGTGGAAGACCTGGACCGCATCGTGGCCTTGACCTC CAACCAGGAGTACCTGGACCTGTCCATGCCCCTGGACCAGTACTCCCCCAGCTTTCCCGACACCCGGAGCTCTACGTGCTCCTCAGGGGAGGATTCCGTC TTCTCTCATGAGCCGCTGCCCGAGGAGCCCTGCCTGCCCCGACACCCAGCCCAGCTTGCCAATGGCGGACTCAAACGCCGCTGACTGCCACCCACACGCC CTCCCCAGACTCCACCGTCAGCTGTAACCCTCACCCACAGCCCCTGCTGGGCCCACCACCTGTCCGTCCCTGTCCCCTTTCCTGCTGGCAGGAGCCGGCT GCCTACCAGGGGCCTTCCTGTGTGGCCTGCCTTCACCCCACTCAGCTCACCTCTCCCTCCACCTCCTCTCCACCTGCTGGTGAGAGGTGCAAAGAGGCAG ATCTTTGCTGCCAGCCACTTCATCCCCTCCCAGATGTTGGACCAACACCCCTCCCTGCCACCAGGCACTGCCTGGAGGGCAGGGAGTGGGAGCCAATGAA CAGGCATGCAAGTGAGAGCTTCCTGAGCTTTCTCCTGTCGGTTTGGTCTGTTTTGCCTTCACCCATAAGCCCCTCGCACTCTGGTGGCAGGTGCCTTGTC CTCAGGGCTACAGCAGTAGGGAGGTCAGTGCTTCGTGCCTCGATTGAAGGTGACCTCTGCCCCAGATAGGTGGTGCCAGTGGCTTATTAATTCCGATACT AGTTTGCTTTGCTGACCAAATGCCTGGTACCAGAGGATGGTGAGGCGAAGGCCAGGTTGGGGGCAGTGTTGTGGCCCTGGGGCCCAGCCCCAAACTGGGG GCTCTGTATATAGCTATGAAGAAAACACAAAGTGTATAAATCTGAGTATATATTTACATGTCTTTTTAAAAGGGTCGTTACCAGAGATTTACCCATCGGG TAAGATGCTCCTGGTGGCTGGGAGGCATCAGTTGCTATATATTAAAAACAAAAAAGAAAAAAAAGGAAAATGTTTTTAAAAAGGTCATATATTTTTTGCT ACTTTTGCTGTTTTATTTTTTTAAATTATGTTCTAAACCTATTTTCAGTTTAGGTCCCTCAATAAAAATTGCTGCTGCTTC

FGFR1.F11 (forward primer)
FGFR1.R11 (reverse primer)
Single exon skipping

Frame 0

PRRRIALGALLRPQARRPRRRAAASGSRDAGAA AARGGTRVCRELGGHVRTGPRPLVAHCGDLAFPG RERAAA*KAAEPKDFSPVRARGAPQGARYPCCS RARRGAGASAGRWSPVCKESEAPPLRSGGGGHK VWRPRVADGSPPPAPPPGHQLRLHCSRPGWRRRA PSAAGSRAPAAELLRPRQDPNRARGRRAGAGDA GTRPLAQATADSPEAEPPRRARVSLKRRIELTV EYPWRCGALSPTSNCRTGMWSWKCLLFWAVLVTA TLCTARPSPTLPEQGWQL*PGISVRCFCTLAGS CQPKPSPGEPLWKWSPSWSTPVTCCSFAVGCGT MCRASTGCGTGCSWRKATAPASQGRRWRCRTPCP QTPASMLA*PAAPRAVTPPTSPSMFQMLSPPRR MMMMMMTPLQRRKKQITPNQTVCP*LHIGHPQK RWKRNCMQCRLPRQ*SSNALPVGPQTPHCAG*KM AKNSNLTTELEATRSVMPPGAS*WTLWCPLTRA TTPALWRMSTAASTTHTSWMSWSGPLTGPSCKQ GCPPTKQWPWVATWSSCVRCTVTRSRTSSG*STS R*MGARLAQTTCLMSRS*RLLELIPPTKRWRCF T*EMSPLRTQGSIRAWRVTLSDSPITLHG*PFW KPWKRGRQ**PRPCTWRSSSIAQGPSSSPAWWGR SSSTR*RVVPRRVTSTARWLCTSWPRASLCADR CLLTPVHP*TLGFFWFGHHGSPPVGLPC*QGSL SMSFPKTLAGSCLGTDWS*ANPWERAALGRWCWQ RLSGWTRTNPTV*PKWL*RC*SRTQQRKTCQT* SQKWR**R*SGSIRISSTCWGPARRMVPCMSSW SMPPRATCGSTCRPGGPQGWNTATTPATTQRSSS PPRTWCPAPTRWPEAWSIWPPRSAYTETWQPGM SW*QRTM**R*QTLASHGTFTTSTTIKRQPTAD CL*SGWHPRHYLTGSTPTRVMCGLSGCSCGRSSL WAAPHTPVCLWRNFSSC*RRVTAWTSPVTAPTS CT**CGTAGMQCPHRDPPSSSWWKTWTASWP*P PTRSTWTCPCPWTSTPPAFPTPGALRAPQGRIPS SLMSRCPRSPACPDTQPSLPMADSNAADCHPHA LPRLHRQL*PSPTAPAGPTTCPSLSPFLLAGAG CLPGAFLCGLPSPHSAHLSLHLLSTCW*EVQRGR SLLPATSSPPRCWTNTPPCHQALPGGQGVGANE QACK*ELPELSPVGLVCFAFTHKPLALWWQVPC PQGYSSREVSASCLD*R*PLPQIGGASGLLIPIL VCFADQMPGTRGW*GEGQVGGSVVALGPSPKLG ALYIAMKKTQSV*I*VYIYMSF*KGRYQRFTHR VRCSWWLGGISCYILKTKKKKKENVFKKVIYFLL LLLFYFFKLCSKPIFSLGPSIKIAAA

Frame 1

PAGA*RSERSCGHRRGVLGGGRQLAGAGTPVQP QRAEEPGCAGSWAATSGRDRDPS*RIAATSPSP AASAPLLEKPRNPRTFLRSELGAPRRAHGTRAAV GHAAAPGPPQGDGARSARKVRRRRCVLEEGGTR SGDPGWRTGALPPPRLRGTSSGSIVPARAGGAE HRAPPGVERRPRSSCDPARTRTEPGGGGPEPGTR AHARSHKPRRTLPRRNLHAERGSV*KGGSSSLW SIHGDVEPCHQPLTAELGCGAGSASSSGLCWSQ PHSAPLGRPRPCLNKDGSCDPGFR*DASALWPGP ASRSPALGSPCGSGVLPGPPR*PAAASLSAAGR CAEHQLAAGRGAAGGKQPHPHHRGGGGGAGLRA RRLRPLCLRNQQPLGQ*HHLLLRQCFRCSPLLGG ******LLFRGERNR*HQTKPYARSSILDIPRK DGKEIACSAGCQDSEVQMPFQWDPKPHTALVEK WQRIQT*PQNWRLQGPLCHLEHHNGLCGAL*QGQ LHLHCGE*VRQHQPHIPAGCRGAVPSPAHPASR VARQQNSGPG*QRGVHV*GVQ*PAAAHPVAKAH RGEWEQDWPRQPALCPDLEDCWS*YHRQRDGGAS LKKCLL*GRRGVYVLGG*LYRTLPSLCMVDRSG SPGREAGSDDLAPVPGDHHLLHRGLPHLLHGGV GHRLQDEEWYQEE*LPQPDGCAQAGQEHPSAQTG VC*LQCIHELWGSSGSAITALLQWDSHASRGL* V*ASRRPSLGAASGQTGLRQTPGRGLLWAGGVG RGYRAGQGQTQPCDQSGCEDVEVGRNRERLVRPD LRNGDDEDDREA*EYHQPAGGLHAGWSLVCHRG VCLQGQPAGVPAGPEAPRAGILLQPQPQPRGAA LLQGPGVLRLPGGPRHGVSGLQEVHTPRPGSQEC PGDRGQCDEDSRLWPRTGHSPHRLL*KDNQRPT ACEVDGTRGII*PDLHPPE*CVVFRGAPVGDLH SGRLPIPRCACGGTFQAAEGGSPHGQAQ*LHQRA VHDDAGLLACSALTETHLQAAGGRPGPHRGLDL QPGVPGPVHAPGPVLPQLSRHPELYVLLRGGFR LLS*AAARGALPAPTPSPACQWRTQTPLTATHTP SPDSTVSCNPHPQPLLGPPPVRPCPLSCWQEPA AYQGPSCVACLHPTQLTSPSTSSPPAGERCKEA DLCCQPLHPLPDVGPTPLPATRHCLEGREWEPMN RHASESFLSFLLSVWSVLPSPISPSHSGGRCLV LRATAVGRSVLRASIEGDLCPR*VVPVAY*FRY *FALLTKCLVPEDGEAKARLGAVLWPWGPAPNWG LCI*L*RKHKVYKSEYIFTCLFKRVVTRDLPIG *DAPGGWEASVAIY*KQKRKKRKMFLKRSYIFC YFCCFIFLNYVLNLFSV*VPQ*KLLLL

Frame 2

PPAHSARSALAATGAASSAAGGS*REPGRRCSR SARRNPGVPGAGRPRPDGTETPRSALRRPRLPR PRARRCLKSRGTQGLFSGPSSGRPAGRTVPVLQ SGTPRRRGLRRAMEPGLQGK*GAAAAFWRRGAQG LETPGGGREPSPRPASGAPAPAPLFPPGLEAPS TERRRESSAGRGALATPPGPEQSPGAAGRSRGR GHTPARTSHGGLSRGGTSTPSEGQFEKEDRAHCG VSMEMWSLVTNL*LQNWDVELEVPPLLGCAGHS HTLHR*AVPDLA*TRMAAVTRDFGEMLLHSGRV LPAEAQPWGAPVEVESFLVHPGDLLQLRCRLRDD VQSINWLRDGVQLAESNRTRITGEEVEVQDSVP ADSGLYACVTSSPSGSDTTYFSVNVSDALPSSE DDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEK MEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKN GKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKG NYTCIVENEYGSINHTYQLDVVERSPHRPILQAG LPANKTVALGSNVEFMCKVYSDPQPHIQWLKHI EVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVL HLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLE ALEERPAVMTSPLYLEIIIYCTGAFLISCMVGS VIVYKMKSGTKKSDFHSQMAVHKLAKSIPLRRQ VSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSE YELPEDPRWELPRDRLVLGKPLGEGCFGQVVLA EAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDL ISEMEMMKMIGKHKNIINLLGACTQDGPLYVIVE YASKGNLREYLQARRPPGLEYCYNPSHNPEEQL SSKDLVSCAYQVARGMEYLASKKCIHRDLAARN VLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRL PVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFT LGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNE LYMMMRDCWHAVPSQRPTFKQLVEDLDRIVALTS NQEYLDLSMPLDQYSPSFPDTRSSTCSSGEDSV FSHEPLPEEPCLPRHPAQLANGGLKRR*LPPTR PPQTPPSAVTLTHSPCWAHHLSVPVPFPAGRSRL PTRGLPVWPAFTPLSSPLPPPPLHLLVRGAKRQ IFAASHFIPSQMLDQHPSLPPGTAWRAGSGSQ* TGMQVRAS*AFSCRFGLFCLHP*APRTLVAGALS SGLQQ*GGQCFVPRLKVTSAPDRWCQWLINSDT SLLC*PNAWYQRMVRRRPGWGQCCGPGAQPQTG GSVYSYEENTKCINLSIYLHVFLKGSLPEIYPSG KMLLVAGRHQLLYIKNKKEKKGKCF*KGHIFFA TFAVLFF*IMF*TYFQFRSLNKNCCCF

Sequence FGFR1.iAug05

Frame 0

Frame 1

Frame 2

Sequence FGFR1.fAug05

Frame 0

Frame 1

Frame 2

Sequence FGFR1.cAug05

Frame 0

Frame 1

Frame 2

Sequence FGFR1.bAug05

Frame 0

Frame 1

Frame 2

Sequence FGFR1.aAug05

Frame 0

Frame 1

Frame 2

Sequence FGFR1.gAug05

Frame 0

Frame 1

Frame 2

Sequence FGFR1.mAug05

Frame 0

Frame 1

Frame 2