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Sequences

Sequence FANCL.dAug05

CGAGCGCCAGCGGACTGCGCATGTGCAGGACCCAGCAGGTCTAGAGCTTTTCTGTGTTTCTCCGGACTTCGAGCCATGGCGGTGACGGAAGCGAGCCTGT TGCGCCAGTGCCCCCTGCTTCTGCCCCAGAACCGGTCGAAAACCGTGTATGAGGGATTCATCTCGGCTCAGACTTGTGTATGCGGATACCTGCTTCAGTA CCATCAAGTTAAAAGCAGAAGATGCTTCTGGTAGAGAGCATTTAATCACTCTCAAGTTGAAGGCAAAGTATCCTGCAGAATCACCAGATTATTTTGTGGA TTTTCCTGTTCCATTTTGTGCCTCCTGGACACCTCAGAGCTCCTTAATAAGCATTTATAGTCAGTTTTTGGCAGCAATAGAATCACTAAAGGCATTCTGG GATGTTATGGATGAAATCGATGAGAAGACCTGGGTACTTGAGCCAGAAAAACCTCCACGGAGTGCAACAGCACGCAGAATTGCATTAGGTAATAATGTTT CCATAAATATAGAGGTAGACCCCAGGCATCCTACTATGCTTCCTGAGTGCTTCTTTCTTGGAGCTGACCATGTGGTAAAACCCCTGGGAATTAAGCTGAG CAGGAACATACATTTGTGGGATCCAGAAAATAGTGTGTTACAAAATTTGAAAGATGTTTTAGAAATTGATTTTCCAGCTCGTGCTATCCTGGAAAAATCT GATTTTACTATGGATTGTGGAATTTGTTATGCTTATCAACTTGACGGTACCATTCCTGATCAAGTGTGTGATAATTCTCAGTGTGGACAACCTTTCCATC AAATATGCTTATATGAGTGGCTGAGAGGACTACTAACTAGTAGACAGAGTTTTAACATCATATTTGGTGAATGTCCATATTGTAGTAAGCCAATTACCTT AAAAATGTCTGGAAGGAAACACTGAAATAAGAATACAACATTTCGGTGAAGAGCTGGAAACTTAAAAAATTATCAAAAGGAATTTTGGTATCATCTTCAG AGAAAAAATAAAGCAAGAAATACTAACATCAAAAGGACAGGTATGATGATGCGATAATAATAAACATCTGCGTTTGTCTCTTCACTAAGAGTAAACTGGG AAATTGTAGGCCAAAGTCCAGTTGAACTTTCTAAGTCTGTGATCCCCGTGCTGACTGTGGAAGTGTATTTATACCAAGATGGAGATCTTGACTTCTTGAA TATATCTGGACTGGTAAAATCTTGATGAGGCTCATAAAATGAGTTTGGGAATTGTGTATAGCTGATTTTTTGTGGGAAACTGTTTACTTCATTCAAAGGT TCTTGAGACTCTTGATATTTCTGTCTTCTCCTTGTGCTTTCCTATGGAAAAAATACATATATAGTTTAGTTTGTTAGACGTGAGTTATCCAAGTATTTAT TTTGTGTAGTGTGTAAGAATGCTAAATAAAATGTTATACAAGATCAA

FANCL.F9 (forward primer)
FANCL.R5 (reverse primer)
Multiple exon skipping

Frame 0

RAPADCACAGPSRSRAFLCFSGLRAMAVTEASL LRQCPLLLPQNRSKTVYEGFISAQTCVCGYLLQY HQVKSRRCFW*RAFNHSQVEGKVSCRITRLFCG FSCSILCLLDTSELLNKHL*SVFGSNRITKGIL GCYG*NR*EDLGT*ARKTSTECNSTQNCIR**CF HKYRGRPQASYYAS*VLLSWS*PCGKTPGN*AE QEHTFVGSRK*CVTKFERCFRN*FSSSCYPGKI *FYYGLWNLLCLST*RYHS*SSV**FSVWTTFPS NMLI*VAERTTN**TEF*HHIW*MSIL**ANYL KNVWKETLK*EYNISVKSWKLKKLSKGILVSSS EKK*SKKY*HQKDRYDDAIIINICVCLFTKSKLG NCRPKSS*TF*VCDPRADCGSVFIPRWRS*LLE YIWTGKILMRLIK*VWELCIADFLWETVYFIQR FLRLLIFLSSPCAFLWKKYIYSLVC*T*VIQVFI LCSV*EC*IKCYTRS

Frame 1

ERQRTAHVQDPAGLELFCVSPDFEPWR*RKRAC CASAPCFCPRTGRKPCMRDSSRLRLVYADTCFS TIKLKAEDASGREHLITLKLKAKYPAESPDYFVD FPVPFCASWTPQSSLISIYSQFLAAIESLKAFW DVMDEIDEKTWVLEPEKPPRSATARRIALGNNV SINIEVDPRHPTMLPECFFLGADHVVKPLGIKLS RNIHLWDPENSVLQNLKDVLEIDFPARAILEKS DFTMDCGICYAYQLDGTIPDQVCDNSQCGQPFH QICLYEWLRGLLTSRQSFNIIFGECPYCSKPITL KMSGRKH*NKNTTFR*RAGNLKNYQKEFWYHLQ RKNKARNTNIKRTGMMMR***TSAFVSSLRVNW EIVGQSPVELSKSVIPVLTVEVYLYQDGDLDFLN ISGLVKS**GS*NEFGNCV*LIFCGKLFTSFKG S*DS*YFCLLLVLSYGKNTYIV*FVRRELSKYL FCVVCKNAK*NVIQDQ

Frame 2

SASGLRMCRTQQV*SFSVFLRTSSHGGDGSEPV APVPPASAPEPVENRV*GIHLGSDLCMRIPASV PSS*KQKMLLVESI*SLSS*RQSILQNHQIILW IFLFHFVPPGHLRAP**AFIVSFWQQ*NH*RHSG MLWMKSMRRPGYLSQKNLHGVQQHAELH*VIMF P*I*R*TPGILLCFLSASFLELTMW*NPWELS* AGTYICGIQKIVCYKI*KMF*KLIFQLVLSWKNL ILLWIVEFVMLINLTVPFLIKCVIILSVDNLSI KYAYMSG*EDY*LVDRVLTSYLVNVHIVVSQLP *KCLEGNTEIRIQHFGEELET*KIIKRNFGIIFR EKIKQEILTSKGQV**CDNNKHLRLSLH*E*TG KL*AKVQLNFLSL*SPC*LWKCIYTKMEILTS* IYLDW*NLDEAHKMSLGIVYS*FFVGNCLLHSKV LETLDISVFSLCFPMEKIHI*FSLLDVSYPSIY FV*CVRMLNKMLYKI

Sequence FANCL.jAug05

CGAGCGCCAGCGGACTGCGCATGTGCAGGACCCAGCAGGTCTAGAGCTTTTCTGTGTTTCTCCGGACTTCGAGCCATGGCGGTGACGGAAGCGAGCCTGT TGCGCCAGTGCCCCCTGCTTCTGCCCCAGAACCGGTCGAAAACCGTGTATGAGGGATTCATCTCGGCTCAGACTTGTGTATGCGGATACCTGCTTCAGTA CCATCAAGTTAAAAGCAGAAGATGCTTCTGGTAGAGAGCATTTAATCACTCTCAAGTTGAAGGCAAAGTATCCTGCAGAATCACCAGATTATTTTGTGGA TTTTCCTGTTCCATTTTGTGCCTCCTGGACACCTCAGGTAAATTCTCCTCAGAGCTCCTTAATAAGCATTTATAGTCAGTTTTTGGCAGCAATAGAATCA CTAAAGGCATTCTGGGATGTTATGGATGAAATCGATGAGAAGACCTGGGTACTTGAGCCAGAAAAACCTCCACGGAGTGCAACAGCACGCAGAATTGCAT TAGGTAATAATGTTTCCATAAATATAGAGGTAGACCCCAGGCATCCTACTATGCTTCCTGAGTGCTTCTTTCTTGGAGCTGACCATGGTATGACATCTGA GATTTAAACGTTTTTTAATAGAGTCATAAAATCCTTGCCTAGCATTGTTAAATTATTAGACAATATTAGTATATTAATGGTAGTTTTTATTTTTTGTCTG AGACGGAGTTTTGCTCTTGTTGCCCAGTATGTAGTGTAATGGCGCGATCTCGGTTCACCGCAATAATGATAGTTATTTTAAATGACAATGTCTCAGCATT CTGAACTGTATAATTTTGTTTTTTAACATAATACATATAATTTTGATTTTCTAATCAAGTAGTTAAGAAACATTCTGTATCAAATGTGGCTTTAGAAACC AAGAGTAGAGCATGCAATTATTAAATTTTTTTTTTTTAATCTGAAGTGGTAAAACCCCTGGGAATTAAGCTGAGCAGGAACATACATTTGTGGTAGGTAC CTTGGCAATATAATTTTTACTTTTTGGTGATTTTACAAAGTTCACAGCATAATTTTTTTCCCCATGCTTTCAGGGATCCAGAAAATAGTGTGTTACAAAA TTTGAAAGATGTTTTAGAAATTGATTTTCCAGCTCGTGCTATCCTGGAAAAATCTGTAAGTTTTATCAGTGTTTTGATATTCCAGACAGTATTGCATAAT GAAGTTTAAATGCTTCTCTCAGTGAAAAATGAGGCTGAAAAAGGAGGGAATTAGAAAAATATTATAACACAGACTTCTGATCTGCATCAAAAAACTTGTT TACCTTTGATTGGTTGTTAAGTACAACTGTCTCTTGGAACAGGGGGATTGATTTCAGGGCTGCCCATGGATACAAAAATCCACAGACATTAAAGTTCCAC AGTTACCCTGCAGGACCCATATATAGAAGAAGTCGGCCCTCTGTATACAGAGATTTCACATAAACACAGGTTCTGCATCTTGTCAGTACTGTATTTTTGA TCTGCATTTGGTTAAAAAAAAAATCTGTGTATAAGTAGACCTGTGCAGTTCAAACCCATGTTGTTCAAGGGTCAACTATATACTTTTTTCATGGTCTTCA GTAGTGACGTCATTAATGACAGACATTTATTAAAAATAAGGGG

FANCL.F9 (forward primer)
FANCL.R5 (reverse primer)
Multiple exon skipping

Frame 0

RAPADCACAGPSRSRAFLCFSGLRAMAVTEASL LRQCPLLLPQNRSKTVYEGFISAQTCVCGYLLQY HQVKSRRCFW*RAFNHSQVEGKVSCRITRLFCG FSCSILCLLDTSGKFSSELLNKHL*SVFGSNRI TKGILGCYG*NR*EDLGT*ARKTSTECNSTQNCI R**CFHKYRGRPQASYYAS*VLLSWS*PWYDI* DLNVF**SHKILA*HC*IIRQY*YINGSFYFLS ETEFCSCCPVCSVMARSRFTAIMIVILNDNVSAF *TV*FCFLT*YI*F*FSNQVVKKHSVSNVALET KSRACNY*IFFF*SEVVKPLGIKLSRNIHLW*V PWQYNFYFLVILQSSQHNFFPHAFRDPENSVLQN LKDVLEIDFPARAILEKSVSFISVLIFQTVLHN EV*MLLSVKNEAEKGGN*KNIITQTSDLHQKTC LPLIGC*VQLSLGTGGLISGLPMDTKIHRH*SST VTLQDPYIEEVGPLYTEISHKHRFCILSVLYF* SAFG*KKNLCISRPVQFKPMLFKGQLYTFFMVF SSDVINDRHLLKIR

Frame 1

ERQRTAHVQDPAGLELFCVSPDFEPWR*RKRAC CASAPCFCPRTGRKPCMRDSSRLRLVYADTCFS TIKLKAEDASGREHLITLKLKAKYPAESPDYFVD FPVPFCASWTPQVNSPQSSLISIYSQFLAAIES LKAFWDVMDEIDEKTWVLEPEKPPRSATARRIA LGNNVSINIEVDPRHPTMLPECFFLGADHGMTSE I*TFFNRVIKSLPSIVKLLDNISILMVVFIFCL RRSFALVAQYVV*WRDLGSPQ***LF*MTMSQH SELYNFVF*HNTYNFDFLIK*LRNILYQMWL*KP RVEHAIIKFFFFNLKW*NPWELS*AGTYICGRY LGNIIFTFW*FYKVHSIIFFPMLSGIQKIVCYK I*KMF*KLIFQLVLSWKNL*VLSVF*YSRQYCIM KFKCFSQ*KMRLKKEGIRKIL*HRLLICIKKLV YL*LVVKYNCLLEQGD*FQGCPWIQKSTDIKVP QLPCRTHI*KKSALCIQRFHINTGSASCQYCIFD LHLVKKKICV*VDLCSSNPCCSRVNYILFSWSS VVTSLMTDIY*K*G

Frame 2

SASGLRMCRTQQV*SFSVFLRTSSHGGDGSEPV APVPPASAPEPVENRV*GIHLGSDLCMRIPASV PSS*KQKMLLVESI*SLSS*RQSILQNHQIILW IFLFHFVPPGHLR*ILLRAP**AFIVSFWQQ*NH *RHSGMLWMKSMRRPGYLSQKNLHGVQQHAELH *VIMFP*I*R*TPGILLCFLSASFLELTMV*HL RFKRFLIES*NPCLALLNY*TILVY*W*FLFFV* DGVLLLLPSM*CNGAISVHRNNDSYFK*QCLSI LNCIILFFNIIHIILIF*SSS*ETFCIKCGFRN QE*SMQLLNFFFLI*SGKTPGN*AEQEHTFVVGT LAI*FLLFGDFTKFTA*FFSPCFQGSRK*CVTK FERCFRN*FSSSCYPGKICKFYQCFDIPDSIA* *SLNASLSEK*G*KRRELEKYYNTDF*SASKNLF TFDWLLSTTVSWNRGIDFRAAHGYKNPQTLKFH SYPAGPIYRRSRPSVYRDFT*TQVLHLVSTVFL ICIWLKKKSVYK*TCAVQTHVVQGSTIYFFHGLQ **RH**QTFIKNKG

Sequence FANCL.hAug05

FANCL.F9 (forward primer)
FANCL.R5 (reverse primer)
Multiple exon skipping

Frame 0

RAPADCACAGPSRSRAFLCFSGLRAMAVTEASL LRQCPLLLPQNRSKTVYEGFISAQGRDFHLRIVL PEDLQLKNARLLCSWQLRTILSGYHRIVQQRMQ HSPDLMSFMMELKMLLEVALKNRQEL*LVYADT CFSTIKLKAEDASGREHLITLKLKAKYPAESPDY FVDFPVPFCASWTPQVNSPQSSLISIYSQFLAA IESLKAFWDVMDEIDEKTWVLEPE

Frame 1

ERQRTAHVQDPAGLELFCVSPDFEPWR*RKRAC CASAPCFCPRTGRKPCMRDSSRLREETSTLG*C CLKIYN*RMQDYYVVGS*EQYLVDTIE*YNRECS TLLI**AL*WS*RCFWKLP*RIDKSCNLCMRIP ASVPSS*KQKMLLVESI*SLSS*RQSILQNHQI ILWIFLFHFVPPGHLR*ILLRAP**AFIVSFWQQ *NH*RHSGMLWMKSMRRPGYLSQK

Frame 2

SASGLRMCRTQQV*SFSVFLRTSSHGGDGSEPV APVPPASAPEPVENRV*GIHLGSGKRLPP*DSV A*RFTTEECKIIM*LAAENNT*WIPSNSTTENA ALS*SNELYDGVEDAFGSCLKE*TRAVTCVCGYL LQYHQVKSRRCFW*RAFNHSQVEGKVSCRITRL FCGFSCSILCLLDTSGKFSSELLNKHL*SVFGS NRITKGILGCYG*NR*EDLGT*ARK