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Sequences

Sequence FANCL.dAug05

CGAGCGCCAGCGGACTGCGCATGTGCAGGACCCAGCAGGTCTAGAGCTTTTCTGTGTTTCTCCGGACTTCGAGCCATGGCGGTGACGGAAGCGAGCCTGT
TGCGCCAGTGCCCCCTGCTTCTGCCCCAGAACCGGTCGAAAACCGTGTATGAGGGATTCATCTCGGCTCAGACTTGTGTATGCGGATACCTGCTTCAGTA
CCAT
CAAGTTAAAAGCAGAAGATGCTTCTGGTAGAGAGCATTTAATCACTCTCAAGTTGAAGGCAAAGTATCCTGCAGAATCACCAGATTATTTTGTGGA
TTTTCCTGTTCCATTTTGTGCCTCCTGGACACCTCAGAGCTCCTTAATAAGCATTTATAGTCAGTTTTTGGCAGCAATAGAATCACTAAAGGCATTCTGG
GATGTTATGGATGAAATCGATGAGAAGACCTGGGTACTTGAGCCAGAAAAACCTCCACGGAGTGCAACAGCACGCAGAATTGCATTAGGTAATAATGTTT
CCATAAATATAGAGGTAGACCCCAGGCATCCTACTATGCTTCCTGAGTGCTTCTTTCTTGGAGCTGACCATGTGGTAAAACCCCTGGGAATTAAGCTGAG
CAGGAACATACATTTGTGGGATCCAGAAAATAGTGTGTTACAAAATTTGAAAGATGTTTTAGAAATTGATTTTCCAGCTCGTGCTATCCTGGAAAAATCT
GATTTTACTATGGATTGTGGAATTTGTTATGCTTATCAACTTGACGGTACCATTCCTGATCAAGTGTGTGATAATTCTCAGTGTGGACAACCTTTCCATC
AAATATGCTTATATGAGTGGCTGAGAGGACTACTAACTAGTAGACAGAGTTTTAACATCATATTTGGTGAATGTCCATATTGTAGTAAGCCAATTACCTT
AAAAATGTCTGGAAGGAAACACTGAAATAAGAATACAACATTTCGGTGAAGAGCTGGAAACTTAAAAAATTATCAAAAGGAATTTTGGTATCATCTTCAG
AGAAAAAATAAAGCAAGAAATACTAACATCAAAAGGACAGGTATGATGATGCGATAATAATAAACATCTGCGTTTGTCTCTTCACTAAGAGTAAACTGGG
AAATTGTAGGCCAAAGTCCAGTTGAACTTTCTAAGTCTGTGATCCCCGTGCTGACTGTGGAAGTGTATTTATACCAAGATGGAGATCTTGACTTCTTGAA
TATATCTGGACTGGTAAAATCTTGATGAGGCTCATAAAATGAGTTTGGGAATTGTGTATAGCTGATTTTTTGTGGGAAACTGTTTACTTCATTCAAAGGT
TCTTGAGACTCTTGATATTTCTGTCTTCTCCTTGTGCTTTCCTATGGAAAAAATACATATATAGTTTAGTTTGTTAGACGTGAGTTATCCAAGTATTTAT
TTTGTGTAGTGTGTAAGAATGCTAAATAAAATGTTATACAAGATCAA
  • FANCL.F9 (forward primer)
  • FANCL.R5 (reverse primer)
  • Single exon skipping

Frame 0

RAPADCACAGPSRSRAFLCFSGLRAMAVTEASL
LRQCPLLLPQNRSKTVYEGFISAQTCVCGYLLQY
H
QVKSRRCFW*RAFNHSQVEGKVSCRITRLFCG
FSCSILCLLDTSELLNKHL*SVFGSNRITKGIL
GCYG*NR*EDLGT*ARKTSTECNSTQNCIR**CF
HKYRGRPQASYYAS*VLLSWS*PCGKTPGN*AE
QEHTFVGSRK*CVTKFERCFRN*FSSSCYPGKI
*FYYGLWNLLCLST*RYHS*SSV**FSVWTTFPS
NMLI*VAERTTN**TEF*HHIW*MSIL**ANYL
KNVWKETLK*EYNISVKSWKLKKLSKGILVSSS
EKK*SKKY*HQKDRYDDAIIINICVCLFTKSKLG
NCRPKSS*TF*VCDPRADCGSVFIPRWRS*LLE
YIWTGKILMRLIK*VWELCIADFLWETVYFIQR
FLRLLIFLSSPCAFLWKKYIYSLVC*T*VIQVFI
LCSV*EC*IKCYTRS

Frame 1

ERQRTAHVQDPAGLELFCVSPDFEPWR*RKRAC
CASAPCFCPRTGRKPCMRDSSRLRLVYADTCFS
TI
KLKAEDASGREHLITLKLKAKYPAESPDYFVD
FPVPFCASWTPQSSLISIYSQFLAAIESLKAFW
DVMDEIDEKTWVLEPEKPPRSATARRIALGNNV
SINIEVDPRHPTMLPECFFLGADHVVKPLGIKLS
RNIHLWDPENSVLQNLKDVLEIDFPARAILEKS
DFTMDCGICYAYQLDGTIPDQVCDNSQCGQPFH
QICLYEWLRGLLTSRQSFNIIFGECPYCSKPITL
KMSGRKH*NKNTTFR*RAGNLKNYQKEFWYHLQ
RKNKARNTNIKRTGMMMR***TSAFVSSLRVNW
EIVGQSPVELSKSVIPVLTVEVYLYQDGDLDFLN
ISGLVKS**GS*NEFGNCV*LIFCGKLFTSFKG
S*DS*YFCLLLVLSYGKNTYIV*FVRRELSKYL
FCVVCKNAK*NVIQDQ

Frame 2

SASGLRMCRTQQV*SFSVFLRTSSHGGDGSEPV
APVPPASAPEPVENRV*GIHLGSDLCMRIPASV
P
SS*KQKMLLVESI*SLSS*RQSILQNHQIILW
IFLFHFVPPGHLRAP**AFIVSFWQQ*NH*RHSG
MLWMKSMRRPGYLSQKNLHGVQQHAELH*VIMF
P*I*R*TPGILLCFLSASFLELTMW*NPWELS*
AGTYICGIQKIVCYKI*KMF*KLIFQLVLSWKNL
ILLWIVEFVMLINLTVPFLIKCVIILSVDNLSI
KYAYMSG*EDY*LVDRVLTSYLVNVHIVVSQLP
*KCLEGNTEIRIQHFGEELET*KIIKRNFGIIFR
EKIKQEILTSKGQV**CDNNKHLRLSLH*E*TG
KL*AKVQLNFLSL*SPC*LWKCIYTKMEILTS*
IYLDW*NLDEAHKMSLGIVYS*FFVGNCLLHSKV
LETLDISVFSLCFPMEKIHI*FSLLDVSYPSIY
FV*CVRMLNKMLYKI

Sequence FANCL.jAug05

CGAGCGCCAGCGGACTGCGCATGTGCAGGACCCAGCAGGTCTAGAGCTTTTCTGTGTTTCTCCGGACTTCGAGCCATGGCGGTGACGGAAGCGAGCCTGT
TGCGCCAGTGCCCCCTGCTTCTGCCCCAGAACCGGTCGAAAACCGTGTATGAGGGATTCATCTCGGCTCAGACTTGTGTATGCGGATACCTGCTTCAGTA
CCAT
CAAGTTAAAAGCAGAAGATGCTTCTGGTAGAGAGCATTTAATCACTCTCAAGTTGAAGGCAAAGTATCCTGCAGAATCACCAGATTATTTTGTGGA
TTTTCCTGTTCCATTTTGTGCCTCCTGGACACCTCAGGTAAATTCTCCTCAGAGCTCCTTAATAAGCATTTATAGTCAGTTTTTGGCAGCAATAGAATCA
CTAAAGGCATTCTGGGATGTTATGGATGAAATCGATGAGAAGACCTGGGTACTTGAGCCAGAAAAACCTCCACGGAGTGCAACAGCACGCAGAATTGCAT
TAGGTAATAATGTTTCCATAAATATAGAGGTAGACCCCAGGCATCCTACTATGCTTCCTGAGTGCTTCTTTCTTGGAGCTGACCATGGTATGACATCTGA
GATTTAAACGTTTTTTAATAGAGTCATAAAATCCTTGCCTAGCATTGTTAAATTATTAGACAATATTAGTATATTAATGGTAGTTTTTATTTTTTGTCTG
AGACGGAGTTTTGCTCTTGTTGCCCAGTATGTAGTGTAATGGCGCGATCTCGGTTCACCGCAATAATGATAGTTATTTTAAATGACAATGTCTCAGCATT
CTGAACTGTATAATTTTGTTTTTTAACATAATACATATAATTTTGATTTTCTAATCAAGTAGTTAAGAAACATTCTGTATCAAATGTGGCTTTAGAAACC
AAGAGTAGAGCATGCAATTATTAAATTTTTTTTTTTTAATCTGAAGTGGTAAAACCCCTGGGAATTAAGCTGAGCAGGAACATACATTTGTGGTAGGTAC
CTTGGCAATATAATTTTTACTTTTTGGTGATTTTACAAAGTTCACAGCATAATTTTTTTCCCCATGCTTTCAGGGATCCAGAAAATAGTGTGTTACAAAA
TTTGAAAGATGTTTTAGAAATTGATTTTCCAGCTCGTGCTATCCTGGAAAAATCTGTAAGTTTTATCAGTGTTTTGATATTCCAGACAGTATTGCATAAT
GAAGTTTAAATGCTTCTCTCAGTGAAAAATGAGGCTGAAAAAGGAGGGAATTAGAAAAATATTATAACACAGACTTCTGATCTGCATCAAAAAACTTGTT
TACCTTTGATTGGTTGTTAAGTACAACTGTCTCTTGGAACAGGGGGATTGATTTCAGGGCTGCCCATGGATACAAAAATCCACAGACATTAAAGTTCCAC
AGTTACCCTGCAGGACCCATATATAGAAGAAGTCGGCCCTCTGTATACAGAGATTTCACATAAACACAGGTTCTGCATCTTGTCAGTACTGTATTTTTGA
TCTGCATTTGGTTAAAAAAAAAATCTGTGTATAAGTAGACCTGTGCAGTTCAAACCCATGTTGTTCAAGGGTCAACTATATACTTTTTTCATGGTCTTCA
GTAGTGACGTCATTAATGACAGACATTTATTAAAAATAAGGGG
  • FANCL.F9 (forward primer)
  • FANCL.R5 (reverse primer)
  • Single exon skipping

Frame 0

RAPADCACAGPSRSRAFLCFSGLRAMAVTEASL
LRQCPLLLPQNRSKTVYEGFISAQTCVCGYLLQY
H
QVKSRRCFW*RAFNHSQVEGKVSCRITRLFCG
FSCSILCLLDTSGKFSSELLNKHL*SVFGSNRI
TKGILGCYG*NR*EDLGT*ARKTSTECNSTQNCI
R**CFHKYRGRPQASYYAS*VLLSWS*PWYDI*
DLNVF**SHKILA*HC*IIRQY*YINGSFYFLS
ETEFCSCCPVCSVMARSRFTAIMIVILNDNVSAF
*TV*FCFLT*YI*F*FSNQVVKKHSVSNVALET
KSRACNY*IFFF*SEVVKPLGIKLSRNIHLW*V
PWQYNFYFLVILQSSQHNFFPHAFRDPENSVLQN
LKDVLEIDFPARAILEKSVSFISVLIFQTVLHN
EV*MLLSVKNEAEKGGN*KNIITQTSDLHQKTC
LPLIGC*VQLSLGTGGLISGLPMDTKIHRH*SST
VTLQDPYIEEVGPLYTEISHKHRFCILSVLYF*
SAFG*KKNLCISRPVQFKPMLFKGQLYTFFMVF
SSDVINDRHLLKIR

Frame 1

ERQRTAHVQDPAGLELFCVSPDFEPWR*RKRAC
CASAPCFCPRTGRKPCMRDSSRLRLVYADTCFS
TI
KLKAEDASGREHLITLKLKAKYPAESPDYFVD
FPVPFCASWTPQVNSPQSSLISIYSQFLAAIES
LKAFWDVMDEIDEKTWVLEPEKPPRSATARRIA
LGNNVSINIEVDPRHPTMLPECFFLGADHGMTSE
I*TFFNRVIKSLPSIVKLLDNISILMVVFIFCL
RRSFALVAQYVV*WRDLGSPQ***LF*MTMSQH
SELYNFVF*HNTYNFDFLIK*LRNILYQMWL*KP
RVEHAIIKFFFFNLKW*NPWELS*AGTYICGRY
LGNIIFTFW*FYKVHSIIFFPMLSGIQKIVCYK
I*KMF*KLIFQLVLSWKNL*VLSVF*YSRQYCIM
KFKCFSQ*KMRLKKEGIRKIL*HRLLICIKKLV
YL*LVVKYNCLLEQGD*FQGCPWIQKSTDIKVP
QLPCRTHI*KKSALCIQRFHINTGSASCQYCIFD
LHLVKKKICV*VDLCSSNPCCSRVNYILFSWSS
VVTSLMTDIY*K*G

Frame 2

SASGLRMCRTQQV*SFSVFLRTSSHGGDGSEPV
APVPPASAPEPVENRV*GIHLGSDLCMRIPASV
P
SS*KQKMLLVESI*SLSS*RQSILQNHQIILW
IFLFHFVPPGHLR*ILLRAP**AFIVSFWQQ*NH
*RHSGMLWMKSMRRPGYLSQKNLHGVQQHAELH
*VIMFP*I*R*TPGILLCFLSASFLELTMV*HL
RFKRFLIES*NPCLALLNY*TILVY*W*FLFFV*
DGVLLLLPSM*CNGAISVHRNNDSYFK*QCLSI
LNCIILFFNIIHIILIF*SSS*ETFCIKCGFRN
QE*SMQLLNFFFLI*SGKTPGN*AEQEHTFVVGT
LAI*FLLFGDFTKFTA*FFSPCFQGSRK*CVTK
FERCFRN*FSSSCYPGKICKFYQCFDIPDSIA*
*SLNASLSEK*G*KRRELEKYYNTDF*SASKNLF
TFDWLLSTTVSWNRGIDFRAAHGYKNPQTLKFH
SYPAGPIYRRSRPSVYRDFT*TQVLHLVSTVFL
ICIWLKKKSVYK*TCAVQTHVVQGSTIYFFHGLQ
**RH**QTFIKNKG

Sequence FANCL.gAug05

CGAGCGCCAGCGGACTGCGCATGTGCAGGACCCAGCAGGTCTAGAGCTTTTCTGTGTTTCTCCGGACTTCGAGCCATGGCGGTGACGGAAGCGAGCCTGT
TGCGCCAGTGCCCCCTGCTTCTGCCCCAGAACCGGTCGAAAACCGTGTATGAGGGATTCATCTCGGCTCAGGAAGTTGCCTTAAAGAATAGACAAGAGCT
GTATGCACTACCTCCTCCTCCCCAGTTCTACTCAAGCCTTATTGAAGAGATAGGAACTCTTGGTTGGGATAA
ACTTGTGTATGCGGATACCTGCTTCAGT
ACCAT
CAAGTTAAAAGCAGAAGATGCTTCTGGTAGAGAGCATTTAATCACTCTCAAGTTGAAGGCAAAGTATCCTGCAGAATCACCAGATTATTTTGTGG
ATTTTCCTGTTCCATTTTGTGCCTCCTGGACACCTCAGAGCTCCTTAATAAGCATTTATAGTCAGTTTTTGGCAGCAATAGAATCACTAAAGGCATTCTG
GGATGTTATGGATGAAATCGATGAGAAGACCTGGGTACTTGAGCCAGAAAAACCTCCACGGAGTGCAACAGCACGCAGAATTGCATTAGGTAATAATGTT
TCCATAAATATAGAGGTAGACCCCAGGCATCCTACTATGCTTCCTGAGTGCTTCTTTCTTGGAGCTGACCATGTGGTAAAACCCCTGGGAATTAAGCTGA
GCAGGAACATACATTTGTGGTAGGTACCTTGGCAATATAATTTTTACTTTTTGGTGATTTTACAAAGTTCACAGCATAATTTTTTTCCCCATGCTTTCAG
GGATCCAGAAAATAGTGTGTTACAAAATTTGAAAGATGTTTTAGAAATTGATTTTCCAGCTCGTGCTATCCTGGAAAAATCTGTAAGTTTTATCAGTGTT
TTGATATTCCAGACAGTATTGCATAATGAAGTTTAAATGCTTCTCTCAGTGAAAAATGAGGCTGAAAAAGGAGGGAATTAGAAAAATATTATAACACAGA
CTTCTGATCTGCATCAAAAAACTTGTTTACCTTTGATTGGTTGTTAAGTACAACTGTCTCTTGGAACAGGGGGATTGATTTCAGGGCTGCCCATGGATAC
AAAAATCCACAGACATTAAAGTTCCACAGTTACCCTGCAGGACCCATATATAGAAGAAGTCGGCCCTCTGTATACAGAGATTTCACATAAACACAGGTTC
TGCATCTTGTCAGTACTGTATTTTTGATCTGCATTTGGTTAAAAAAAAAATCTGTGTATAAGTAGACCTGTGCAGTTCAAACCCATGTTGTTCAAGGGTC
AACTATATACTTTTTTCATGGTCTTCAGTAGTGACGTCATTAATGACAGACATTTATTAAAAATAAGGGG
  • FANCL.F9 (forward primer)
  • FANCL.R5 (reverse primer)
  • Single exon skipping

Frame 0

RAPADCACAGPSRSRAFLCFSGLRAMAVTEASL
LRQCPLLLPQNRSKTVYEGFISAQEVALKNRQEL
YALPPPPQFYSSLIEEIGTLGWDK
LVYADTCFS
TI
KLKAEDASGREHLITLKLKAKYPAESPDYFV
DFPVPFCASWTPQSSLISIYSQFLAAIESLKAFW
DVMDEIDEKTWVLEPEKPPRSATARRIALGNNV
SINIEVDPRHPTMLPECFFLGADHVVKPLGIKL
SRNIHLW*VPWQYNFYFLVILQSSQHNFFPHAFR
DPENSVLQNLKDVLEIDFPARAILEKSVSFISV
LIFQTVLHNEV*MLLSVKNEAEKGGN*KNIITQ
TSDLHQKTCLPLIGC*VQLSLGTGGLISGLPMDT
KIHRH*SSTVTLQDPYIEEVGPLYTEISHKHRF
CILSVLYF*SAFG*KKNLCISRPVQFKPMLFKG
QLYTFFMVFSSDVINDRHLLKIR

Frame 1

ERQRTAHVQDPAGLELFCVSPDFEPWR*RKRAC
CASAPCFCPRTGRKPCMRDSSRLRKLP*RIDKS
CMHYLLLPSSTQALLKR*ELLVGI
NLCMRIPASV
P
SS*KQKMLLVESI*SLSS*RQSILQNHQIILW
IFLFHFVPPGHLRAP**AFIVSFWQQ*NH*RHS
GMLWMKSMRRPGYLSQKNLHGVQQHAELH*VIMF
P*I*R*TPGILLCFLSASFLELTMW*NPWELS*
AGTYICGRYLGNIIFTFW*FYKVHSIIFFPMLS
GIQKIVCYKI*KMF*KLIFQLVLSWKNL*VLSVF
*YSRQYCIMKFKCFSQ*KMRLKKEGIRKIL*HR
LLICIKKLVYL*LVVKYNCLLEQGD*FQGCPWI
QKSTDIKVPQLPCRTHI*KKSALCIQRFHINTGS
ASCQYCIFDLHLVKKKICV*VDLCSSNPCCSRV
NYILFSWSSVVTSLMTDIY*K*G

Frame 2

SASGLRMCRTQQV*SFSVFLRTSSHGGDGSEPV
APVPPASAPEPVENRV*GIHLGSGSCLKE*TRA
VCTTSSSPVLLKPY*RDRNSWLG*
TCVCGYLLQ
YH
QVKSRRCFW*RAFNHSQVEGKVSCRITRLFCG
FSCSILCLLDTSELLNKHL*SVFGSNRITKGIL
GCYG*NR*EDLGT*ARKTSTECNSTQNCIR**C
FHKYRGRPQASYYAS*VLLSWS*PCGKTPGN*AE
QEHTFVVGTLAI*FLLFGDFTKFTA*FFSPCFQ
GSRK*CVTKFERCFRN*FSSSCYPGKICKFYQC
FDIPDSIA**SLNASLSEK*G*KRRELEKYYNTD
F*SASKNLFTFDWLLSTTVSWNRGIDFRAAHGY
KNPQTLKFHSYPAGPIYRRSRPSVYRDFT*TQV
LHLVSTVFLICIWLKKKSVYK*TCAVQTHVVQGS
TIYFFHGLQ**RH**QTFIKNKG

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