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Sequences

Sequence FANCL.gAug05

CGAGCGCCAGCGGACTGCGCATGTGCAGGACCCAGCAGGTCTAGAGCTTTTCTGTGTTTCTCCGGACTTCGAGCCATGGCGGTGACGGAAGCGAGCCTGT
TGCGCCAGTGCCCCCTGCTTCTGCCCCAGAACCGGTCGAAAACCGTGTATGAGGGATTCATCTCGGCTCAGGAAGTTGCCTTAAAGAATAGACAAGAGCT
GTATGCACTACCTCCTCCTCCCCAGTTCTACTCAAGCCTTATTGAAGAGATAGGAACTCTTGGTTGGGATAAACTTGTGTATGCGGATACCTGCTTCAGT
ACCATCAAGTTAAAAGCAGAAGATGCTTCTGGTAGAGAGCATTTAATCACTCTCAAGTTGAAGGCAAAGTATCCTGCAGAATCACCAGATTATTTTGTGG
ATTTTCCTGTTCCATTTTGTGCCTCCTGGACACCTCAGAGCTCCTTAATAAGCATTTATAGTCAGTTTTTGGCAGCAATAGAATCACTAAAGGCATTCTG
GGATGTTATGGATGAAATCGATGAGAAGACCTGGGTACTTGAGCCAGAAAAACCTCCACGGAGTGCAACAGCACGCAGAATTGCATTAGGTAATAATGTT
TCCATAAATATAGAGGTAGACCCCAGGCATCCTACTATGCTTCCTGAGTGCTTCTTTCTTGGAGCTGACCATGTGGTAAAACCCCTGGGAATTAAGCTGA
GCAGGAACATACATTTGTGGTAGGTACCTTGGCAATATAATTTTTACTTTTTGGTGATTTTACAAAGTTCACAGCATAATTTTTTTCCCCATGCTTTCAG
GGATCCAGAAAATAGTGTGTTACAAAATTTGAAAGATGTTTTAGAAATTGATTTTCCAGCTCGTGCTATCCTGGAAAAATCTGTAAGTTTTATCAGTGTT
TTGATATTCCAGACAGTATTGCATAATGAAGTTTAAATGCTTCTCTCAGTGAAAAATGAGGCTGAAAAAGGAGGGAATTAGAAAAATATTATAACACAGA
CTTCTGATCTGCATCAAAAAACTTGTTTACCTTTGATTGGTTGTTAAGTACAACTGTCTCTTGGAACAGGGGGATTGATTTCAGGGCTGCCCATGGATAC
AAAAATCCACAGACATTAAAGTTCCACAGTTACCCTGCAGGACCCATATATAGAAGAAGTCGGCCCTCTGTATACAGAGATTTCACATAAACACAGGTTC
TGCATCTTGTCAGTACTGTATTTTTGATCTGCATTTGGTTAAAAAAAAAATCTGTGTATAAGTAGACCTGTGCAGTTCAAACCCATGTTGTTCAAGGGTC
AACTATATACTTTTTTCATGGTCTTCAGTAGTGACGTCATTAATGACAGACATTTATTAAAAATAAGGGG
  • FANCL.F9 (forward primer)
  • FANCL.R5 (reverse primer)
  • Multiple exon skipping

Frame 0

RAPADCACAGPSRSRAFLCFSGLRAMAVTEASL
LRQCPLLLPQNRSKTVYEGFISAQEVALKNRQEL
YALPPPPQFYSSLIEEIGTLGWDKLVYADTCFS
TIKLKAEDASGREHLITLKLKAKYPAESPDYFV
DFPVPFCASWTPQSSLISIYSQFLAAIESLKAFW
DVMDEIDEKTWVLEPEKPPRSATARRIALGNNV
SINIEVDPRHPTMLPECFFLGADHVVKPLGIKL
SRNIHLW*VPWQYNFYFLVILQSSQHNFFPHAFR
DPENSVLQNLKDVLEIDFPARAILEKSVSFISV
LIFQTVLHNEV*MLLSVKNEAEKGGN*KNIITQ
TSDLHQKTCLPLIGC*VQLSLGTGGLISGLPMDT
KIHRH*SSTVTLQDPYIEEVGPLYTEISHKHRF
CILSVLYF*SAFG*KKNLCISRPVQFKPMLFKG
QLYTFFMVFSSDVINDRHLLKIR

Frame 1

ERQRTAHVQDPAGLELFCVSPDFEPWR*RKRAC
CASAPCFCPRTGRKPCMRDSSRLRKLP*RIDKS
CMHYLLLPSSTQALLKR*ELLVGINLCMRIPASV
PSS*KQKMLLVESI*SLSS*RQSILQNHQIILW
IFLFHFVPPGHLRAP**AFIVSFWQQ*NH*RHS
GMLWMKSMRRPGYLSQKNLHGVQQHAELH*VIMF
P*I*R*TPGILLCFLSASFLELTMW*NPWELS*
AGTYICGRYLGNIIFTFW*FYKVHSIIFFPMLS
GIQKIVCYKI*KMF*KLIFQLVLSWKNL*VLSVF
*YSRQYCIMKFKCFSQ*KMRLKKEGIRKIL*HR
LLICIKKLVYL*LVVKYNCLLEQGD*FQGCPWI
QKSTDIKVPQLPCRTHI*KKSALCIQRFHINTGS
ASCQYCIFDLHLVKKKICV*VDLCSSNPCCSRV
NYILFSWSSVVTSLMTDIY*K*G

Frame 2

SASGLRMCRTQQV*SFSVFLRTSSHGGDGSEPV
APVPPASAPEPVENRV*GIHLGSGSCLKE*TRA
VCTTSSSPVLLKPY*RDRNSWLG*TCVCGYLLQ
YHQVKSRRCFW*RAFNHSQVEGKVSCRITRLFCG
FSCSILCLLDTSELLNKHL*SVFGSNRITKGIL
GCYG*NR*EDLGT*ARKTSTECNSTQNCIR**C
FHKYRGRPQASYYAS*VLLSWS*PCGKTPGN*AE
QEHTFVVGTLAI*FLLFGDFTKFTA*FFSPCFQ
GSRK*CVTKFERCFRN*FSSSCYPGKICKFYQC
FDIPDSIA**SLNASLSEK*G*KRRELEKYYNTD
F*SASKNLFTFDWLLSTTVSWNRGIDFRAAHGY
KNPQTLKFHSYPAGPIYRRSRPSVYRDFT*TQV
LHLVSTVFLICIWLKKKSVYK*TCAVQTHVVQGS
TIYFFHGLQ**RH**QTFIKNKG

Sequence FANCL.cAug05

CGAGCGCCAGCGGACTGCGCATGTGCAGGACCCAGCAGGTCTAGAGCTTTTCTGTGTTTCTCCGGACTTCGAGCCATGGCGGTGACGGAAGCGAGCCTGT
TGCGCCAGTGCCCCCTGCTTCTGCCCCAGAACCGGTCGAAAACCGTGTATGAGGGATTCATCTCGGCTCAGGGAAGAGACTTCCACCTTAGGATAGTGTT
GCCTGAAGATTTACAACTGAAGAATGCAAGATTATTATGTAGTTGGCAGCTGAGAACAATACTTAGTGGATACCATCGAATAGTACAACAGGAAGTTGCC
TTAAAGAATAGACAAGAGCTGTATGCACTACCTCCTCCTCCCCAGTTCTACTCAAGCCTTATTGAAGAGATAGGAACTCTTGGTTGGGATAAACTTGTGT
ATGCGGATACCTGCTTCAGTACCATCAAGTTAAAAGCAGAAGATGCTTCTGGTAGAGAGCATTTAATCACTCTCAAGTTGAAGGCAAAGTATCCTGCAGA
ATCACCAGATTATTTTGTGGATTTTCCTGTTCCATTTTGTGCCTCCTGGACACCTCAGAGCTCCTTAATAAGCATTTATAGTCAGTTTTTGGCAGCAATA
GAATCACTAAAGGCATTCTGGGATGTTATGGATGAAATCGATGAGAAGACCTGGGTACTTGAGCCAGAAAAACCTCCACGGAGTGCAACAGCACGCAGAA
TTGCATTAGGTAATAATGTTTCCATAAATATAGAGGTAGACCCCAGGCATCCTACTATGCTTCCTGAGTGCTTCTTTCTTGGAGCTGACCATGTGGTAAA
ACCCCTGGGAATTAAGCTGAGCAGGAACATACATTTGTGGATTTTACTATGGATTGTGGAATTTGTTATGCTTATCAACTTGACGGTACCATTCCTGATC
AAGTGTGTGATAATTCTCAGTGTGGACAACCTTTCCATCAAATATGCTTATATGAGTGGCTGAGAGGACTACTAACTAGTAGACAGAGTTTTAACATCAT
ATTTGGTGAATGTCCATATTGTAGTAAGCCAATTACCTTAAAAATGTCTGGAAGGAAACACTGAAATAAGAATACAACATTTCGGTGAAGAGCTGGAAAC
TTAAAAAATTATCAAAAGGAATTTTGGTATCATCTTCAGAGAAAAAATAAAGCAAGAAATACTAACATCAAAAGGACAGGTATGATGATGCGATAATAAT
AAACATCTGCGTTTGTCTCTTCACTAAGAGTAAACTGGGAAATTGTAGGCCAAAGTCCAGTTGAACTTTCTAAGTCTGTGATCCCCGTGCTGACTGTGGA
AGTGTATTTATACCAAGATGGAGATCTTGACTTCTTGAATATATCTGGACTGGTAAAATCTTGATGAGGCTCATAAAATGAGTTTGGGAATTGTGTATAG
CTGATTTTTTGTGGGAAACTGTTTACTTCATTCAAAGGTTCTTGAGACTCTTGATATTTCTGTCTTCTCCTTGTGCTTTCCTATGGAAAAAATACATATA
TAGTTTAGTTTGTTAGACGTGAGTTATCCAAGTATTTATTTTGTGTAGTGTGTAAGAATGCTAAATAAAATGTTATACAAGATCAA
  • FANCL.F9 (forward primer)
  • FANCL.R5 (reverse primer)
  • Multiple exon skipping

Frame 0

RAPADCACAGPSRSRAFLCFSGLRAMAVTEASL
LRQCPLLLPQNRSKTVYEGFISAQGRDFHLRIVL
PEDLQLKNARLLCSWQLRTILSGYHRIVQQEVA
LKNRQELYALPPPPQFYSSLIEEIGTLGWDKLV
YADTCFSTIKLKAEDASGREHLITLKLKAKYPAE
SPDYFVDFPVPFCASWTPQSSLISIYSQFLAAI
ESLKAFWDVMDEIDEKTWVLEPEKPPRSATARR
IALGNNVSINIEVDPRHPTMLPECFFLGADHVVK
PLGIKLSRNIHLWILLWIVEFVMLINLTVPFLI
KCVIILSVDNLSIKYAYMSG*EDY*LVDRVLTS
YLVNVHIVVSQLP*KCLEGNTEIRIQHFGEELET
*KIIKRNFGIIFREKIKQEILTSKGQV**CDNN
KHLRLSLH*E*TGKL*AKVQLNFLSL*SPC*LW
KCIYTKMEILTS*IYLDW*NLDEAHKMSLGIVYS
*FFVGNCLLHSKVLETLDISVFSLCFPMEKIHI
*FSLLDVSYPSIYFV*CVRMLNKMLYKI

Frame 1

ERQRTAHVQDPAGLELFCVSPDFEPWR*RKRAC
CASAPCFCPRTGRKPCMRDSSRLREETSTLG*C
CLKIYN*RMQDYYVVGS*EQYLVDTIE*YNRKLP
*RIDKSCMHYLLLPSSTQALLKR*ELLVGINLC
MRIPASVPSS*KQKMLLVESI*SLSS*RQSILQ
NHQIILWIFLFHFVPPGHLRAP**AFIVSFWQQ*
NH*RHSGMLWMKSMRRPGYLSQKNLHGVQQHAE
LH*VIMFP*I*R*TPGILLCFLSASFLELTMW*
NPWELS*AGTYICGFYYGLWNLLCLST*RYHS*S
SV**FSVWTTFPSNMLI*VAERTTN**TEF*HH
IW*MSIL**ANYLKNVWKETLK*EYNISVKSWK
LKKLSKGILVSSSEKK*SKKY*HQKDRYDDAIII
NICVCLFTKSKLGNCRPKSS*TF*VCDPRADCG
SVFIPRWRS*LLEYIWTGKILMRLIK*VWELCI
ADFLWETVYFIQRFLRLLIFLSSPCAFLWKKYIY
SLVC*T*VIQVFILCSV*EC*IKCYTRS

Frame 2

SASGLRMCRTQQV*SFSVFLRTSSHGGDGSEPV
APVPPASAPEPVENRV*GIHLGSGKRLPP*DSV
A*RFTTEECKIIM*LAAENNT*WIPSNSTTGSC
LKE*TRAVCTTSSSPVLLKPY*RDRNSWLG*TCV
CGYLLQYHQVKSRRCFW*RAFNHSQVEGKVSCR
ITRLFCGFSCSILCLLDTSELLNKHL*SVFGSN
RITKGILGCYG*NR*EDLGT*ARKTSTECNSTQN
CIR**CFHKYRGRPQASYYAS*VLLSWS*PCGK
TPGN*AEQEHTFVDFTMDCGICYAYQLDGTIPD
QVCDNSQCGQPFHQICLYEWLRGLLTSRQSFNII
FGECPYCSKPITLKMSGRKH*NKNTTFR*RAGN
LKNYQKEFWYHLQRKNKARNTNIKRTGMMMR**
*TSAFVSSLRVNWEIVGQSPVELSKSVIPVLTVE
VYLYQDGDLDFLNISGLVKS**GS*NEFGNCV*
LIFCGKLFTSFKGS*DS*YFCLLLVLSYGKNTY
IV*FVRRELSKYLFCVVCKNAK*NVIQDQ

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