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Sequences

Sequence BTC.bAug05

CTGGTCCGAGGGCAGCCCGAATATGTCCCTGGGTGTGGGTATGGGTGTGGGGCAATTTGGGTGGGAGCAGCGTGGAGGCTCCCAGGACCAAGTCCTGCGC
CTCTTTGGCGGGGTGTGTGCAGGAGGAGGGGGGATAAATAGGAGGCTCCCTCCTCCCGGCGACATTCACGGAGCCGGCCGGCCTCCCGCCCTGGGTGTTT
CCCTGCCTTGTAGCCAGGGTGCCAGCCTGGGAAGTAGTTTCGTTTCCTTCTGCCTCCGGGATTAGTTTCCAGGCACCCTCTCAGGCGCCCGAGGCCCGGG
AAGGGGGCGAAGAAGGAGGGAGACTTGTCTAGGGGCTGCCCGGCCCGGCAGAGCGGGGTTGATGGACCGGGCCGCCCGGTGCAGCGGCGCCAGCTCCCTG
CCACTGCTCCTGGCCCTTGCCCTGGGTCTAGTGATCCTTCACTGTGTGGTGGCAGATGGGAATTCCACCAGAAGTCCTGAAACTAATGGCCTCCTCTGTG
GAGACCCTGAGGAAAACTGTGCAGCTACCACCACACAATCAAAGCGGAAAGGCCACTTCTCTAGGTGCCCCAAGCAATACAAGCATTACTGCATCAAAGG
GAGATGCCGCTTCGTGGTGGCCGAGCAGACGCCCTCCTGTGTCCCTCTTCGGAAACGTCGTAAAAGAAAGAAGAAAGAAGAAGAAATGGAAACTCTGGGT
AAAGATATAACTCCTATCAATGAAGATATTGAAGAGACAAATATTGCTTAAAAGGCTATGAAGTTACCTCCAGGTTGGTGGCAAGCTGCAAAGTGCCTTG
CTCATTTGAAAATGGACAGAATGTGTCTCAGGAAAACAGCTAGTAGACATGAATTTTAAATAATGTATTTACTTTTTATTTGCAACTTTAGTTTGTGTTA
TTATTTTTTAATAAGAACATTAATTATATGTATATTGTCTAGTAATTGGGAAAAAAGCAACTGGTTAGGTAGCAACAACAGAAGGGAAATTTCAATAACC
TTTCACTTAAGTATTGTCACCAGGATTACTAGTCAAAC
  • BTC.F1 (forward primer)
  • BTC.R1 (reverse primer)
  • Single exon skipping

Frame 0

LVRGQPEYVPGCGYGCGAIWVGAAWRLPGPSPA
PLWRGVCRRRGDK*EAPSSRRHSRSRPASRPGCF
PAL*PGCQPGK*FRFLLPPGLVSRHPLRRPRPG
KGAKKEGDLSRGCPARQSGVDGPGRPVQRRQLP
ATAPGPCPGSSDPSLCGGRWEFHQKS*N*WPPLW
RP*GKLCSYHHTIKAERPLL*VPQAIQALLHQR
EMPLRGGRADALLCPSSETS*KKEERRRNGNSG
*RYNSYQ*RY*RDKYCLKGYEVTSRLVASCKVPC
SFENGQNVSQENS**T*ILNNVFTFYLQL*FVL
LFFNKNINYMYIV**LGKKQLVR*QQQKGNFNN
LSLKYCHQDY*SN

Frame 1

WSEGSPNMSLGVGMGVGQFGWEQRGGSQDQVLR
LFGGVCAGGGGINRRLPPPGDIHGAGRPPALGV
SLPCSQGASLGSSFVSFCLRD*FPGTLSGARGPG
RGRRRRETCLGAARPGRAGLMDRAARCSGASSL
PLLLALALGLVILHCVVADGNSTRSPETNGLLC
GDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKG
RCRFVVAEQTPSCVPLRKRRKRKKKEEEMETLG
KDITPINEDIEETNIA*KAMKLPPGWWQAAKCL
AHLKMDRMCLRKTASRHEF*IMYLLFICNFSLCY
YFLIRTLIICILSSNWEKSNWLGSNNRREISIT
FHLSIVTRITSQ

Frame 2

GPRAARICPWVWVWVWGNLGGSSVEAPRTKSCA
SLAGCVQEEGG*IGGSLLPATFTEPAGLPPWVF
PCLVARVPAWEVVSFPSASGISFQAPSQAPEAR
EGGEEGGRLV*GLPGPAERG*WTGPPGAAAPAPC
HCSWPLPWV**SFTVWWQMGIPPEVLKLMASSV
ETLRKTVQLPPHNQSGKATSLGAPSNTSITASK
GDAASWWPSRRPPVSLFGNVVKERRKKKKWKLWV
KI*LLSMKILKRQILLKRL*SYLQVGGKLQSAL
LI*KWTECVSGKQLVDMNFK*CIYFLFATLVCV
IIF**EH*LYVYCLVIGKKATG*VATTEGKFQ*P
FT*VLSPGLLVK

Sequence BTC.aAug05

CTGGTCCGAGGGCAGCCCGAATATGTCCCTGGGTGTGGGTATGGGTGTGGGGCAATTTGGGTGGGAGCAGCGTGGAGGCTCCCAGGACCAAGTCCTGCGC
CTCTTTGGCGGGGTGTGTGCAGGAGGAGGGGGGATAAATAGGAGGCTCCCTCCTCCCGGCGACATTCACGGAGCCGGCCGGCCTCCCGCCCTGGGTGTTT
CCCTGCCTTGTAGCCAGGGTGCCAGCCTGGGAAGTAGTTTCGTTTCCTTCTGCCTCCGGGATTAGTTTCCAGGCACCCTCTCAGGCGCCCGAGGCCCGGG
AAGGGGGCGAAGAAGGAGGGAGACTTGTCTAGGGGCTGCCCGGCCCGGCAGAGCGGGGTTGATGGACCGGGCCGCCCGGTGCAGCGGCGCCAGCTCCCTG
CCACTGCTCCTGGCCCTTGCCCTGGGTCTAGTGATCCTTCACTGTGTGGTGGCAGATGGGAATTCCACCAGAAGTCCTGAAACTAATGGCCTCCTCTGTG
GAGACCCTGAGGAAAACTGTGCAGCTACCACCACACAATCAAAGCGGAAAGGCCACTTCTCTAGGTGCCCCAAGCAATACAAGCATTACTGCATCAAAGG
GAGATGCCGCTTCGTGGTGGCCGAGCAGACGCCCTCCTGTGTCTGTGATGAAGGCTACATTGGAGCAAGGTGTGAGAGAGTTGACTTGTTTTACCTAAGA
GGAGACAGAGGACAGATTCTGGTGATTTGTTTGATAGCAGTTATGGTAGTTTTTATTATTTTGGTCATCGGTGTCTGCACATGCTGTCACCCTCTTCGGA
AACGTCGTAAAAGAAAGAAGAAAGAAGAAGAAATGGAAACTCTGGGTAAAGATATAACTCCTATCAATGAAGATATTGAAGAGACAAATATTGCTTAAAA
GGCTATGAAGTTACCTCCAGGTTGGTGGCAAGCTGCAAAGTGCCTTGCTCATTTGAAAATGGACAGAATGTGTCTCAGGAAAACAGCTAGTAGACATGAA
TTTTAAATAATGTATTTACTTTTTATTTGCAACTTTAGTTTGTGTTATTATTTTTTAATAAGAACATTAATTATATGTATATTGTCTAGTAATTGGGAAA
AAAGCAACTGGTTAGGTAGCAACAACAGAAGGGAAATTTCAATAACCTTTCACTTAAGTATTGTCACCAGGATTACTAGTCAAACAAAAAAGAAAAGTAG
AAAGGAGGTTAGGTCTTAGGAATTGAATTAATAATAAAGCTACCATTTATCAAGCATTTACCATGTGCTAATAAGTTTGAAATATATTATTTCCTTTATT
CCTTTCAGCAATCCATGAGATAGCTATTATAATCCTCATTTCCTACATATGGAAACAGGGCCAAAGAAGTCAAGTCAAATAATCTAATCCAGATTTAGAG
AGTTGGAGATTGGTAGAGACAGACTAGAAATCAAGATTTATCTAAATCAAAATCTATGCTCTTAACCATCATTTTTGTTAGTCTGAATCGGTGCAACCTA
ACTCAATAATATAACCCAAGGTTTTATACTGAACTGTGTTTTGGTATGATTGTGAGATGTCAAGACCGTCTTCCCACAGGCACACAGAATGAGAAATCCT
GGCCTATATTGCTGTTACCATCATGCTTTCTATAGAATTGGCTTTTATTATGTTTCTTTGCTCCTTTATAAATTCTCAATGATGTGACTACATTTTTACA
TTATTAAAGCAAATAGATTGGCCCCATAACTAGGGAAATGAATAGAGATGGAAAAGTAAATGCTTGGCATTCCTTAAGCCCTGGGTAAGTGTTTATGCTC
AATAAAATATAAATAATATTACTGAAAGAGGCTGTTTATATGGGAGTAATGGTCAGTGACAACACTGGAGAGACCAGGTAGGGACCGGTTGTGACCATAT
CCTCATTTATGTTCTTGATGACAGCATATATGATTGCTCTCAGGTAAATTTTGATGCAGTAAATTCATGACCATAACATCTATTACTTGGGACCATCTTG
CCAAGTGCTCCTTTACTCCATGAAAAAACAATGCTTTAATGTTTCTTTTTTCCTGGACTGAGTTTGGCTGACCAACAAAATCTCTCAAGCCATTTTGTTT
TGATCCTAAAATATCAATGGTGGCTCTCGAAATTTGGTAGATCTGATATCAAGAAAGAATTTCAAAGATTTTTATATCTTTTCAAAATGTATTTTATAGT
TGCTTTGCATACACTAAATAACTTTGTTTATGTAAACTGTCAATATTATCATGTAATAAAATAAATTTATTTTTAAAACAAAGTAAACGTGTGGTTACTT
GGGAATTTTTTATTTTACCATGAATTACTAATGAAAATAAATTTGAACACTATTGCTTTTAAACACCTTATGCTTTGTAAACCCTATCCATTAGTGTACT
TGAAAGAATCCATCCTGACCATGTGTTTTCTTTAGGAACTAAAGCATCTGGGAGTGTGTTGGACATCTATGGGACTGTCTTGCCAATATCCTGGCCATAT
TTTTGAACTCCACCTCCAGCTGCATTTGATTGGTCTGAGGTGGGCACTTAATAAACTTGGGC
  • BTC.F1 (forward primer)
  • BTC.R1 (reverse primer)
  • Single exon skipping

Frame 0

LVRGQPEYVPGCGYGCGAIWVGAAWRLPGPSPA
PLWRGVCRRRGDK*EAPSSRRHSRSRPASRPGCF
PAL*PGCQPGK*FRFLLPPGLVSRHPLRRPRPG
KGAKKEGDLSRGCPARQSGVDGPGRPVQRRQLP
ATAPGPCPGSSDPSLCGGRWEFHQKS*N*WPPLW
RP*GKLCSYHHTIKAERPLL*VPQAIQALLHQR
EMPLRGGRADALLCL**RLHWSKV*ES*LVLPK
RRQRTDSGDLFDSSYGSFYYFGHRCLHMLSPSSE
TS*KKEERRRNGNSG*RYNSYQ*RY*RDKYCLK
GYEVTSRLVASCKVPCSFENGQNVSQENS**T*
ILNNVFTFYLQL*FVLLFFNKNINYMYIV**LGK
KQLVR*QQQKGNFNNLSLKYCHQDY*SNKKEK*
KGG*VLGIELIIKLPFIKHLPCANKFEIYYFLY
SFQQSMR*LL*SSFPTYGNRAKEVKSNNLIQI*R
VGDW*RQTRNQDLSKSKSMLLTIIFVSLNRCNL
TQ*YNPRFYTELCFGMIVRCQDRLPTGTQNEKS
WPILLLPSCFL*NWLLLCFFAPL*ILNDVTTFLH
Y*SK*IGPITREMNRDGKVNAWHSLSPG*VFML
NKI*IILLKEAVYMGVMVSDNTGETR*GPVVTI
SSFMFLMTAYMIALR*ILMQ*IHDHNIYYLGPSC
QVLLYSMKKQCFNVSFFLD*VWLTNKISQAILF
*S*NINGGSRNLVDLISRKNFKDFYIFSKCIL*
LLCIH*ITLFM*TVNIIM**NKFIFKTK*TCGYL
GIFYFTMNY**K*I*TLLLLNTLCFVNPIH*CT
*KNPS*PCVFFRN*SIWECVGHLWDCLANILAI
FLNSTSSCI*LV*GGHLINLG

Frame 1

WSEGSPNMSLGVGMGVGQFGWEQRGGSQDQVLR
LFGGVCAGGGGINRRLPPPGDIHGAGRPPALGV
SLPCSQGASLGSSFVSFCLRD*FPGTLSGARGPG
RGRRRRETCLGAARPGRAGLMDRAARCSGASSL
PLLLALALGLVILHCVVADGNSTRSPETNGLLC
GDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKG
RCRFVVAEQTPSCVCDEGYIGARCERVDLFYLR
GDRGQILVICLIAVMVVFIILVIGVCTCCHPLR
KRRKRKKKEEEMETLGKDITPINEDIEETNIA*K
AMKLPPGWWQAAKCLAHLKMDRMCLRKTASRHE
F*IMYLLFICNFSLCYYFLIRTLIICILSSNWE
KSNWLGSNNRREISITFHLSIVTRITSQTKKKSR
KEVRS*ELN***SYHLSSIYHVLISLKYIISFI
PFSNP*DSYYNPHFLHMETGPKKSSQII*SRFR
ELEIGRDRLEIKIYLNQNLCS*PSFLLV*IGAT*
LNNITQGFILNCVLV*L*DVKTVFPQAHRMRNP
GLYCCYHHAFYRIGFYYVSLLLYKFSMM*LHFY
IIKANRLAP*LGK*IEMEK*MLGIP*ALGKCLCS
IKYK*YY*KRLFIWE*WSVTTLERPGRDRL*PY
PHLCS**QHI*LLSGKF*CSKFMTITSITWDHL
AKCSFTP*KNNALMFLFSWTEFG*PTKSLKPFCF
DPKISMVALEIW*I*YQERISKIFISFQNVFYS
CFAYTK*LCLCKLSILSCNKINLFLKQSKRVVT
WEFFILP*ITNENKFEHYCF*TPYAL*TLSISVL
ERIHPDHVFSLGTKASGSVLDIYGTVLPISWPY
F*TPPPAAFDWSEVGT**TW

Frame 2

GPRAARICPWVWVWVWGNLGGSSVEAPRTKSCA
SLAGCVQEEGG*IGGSLLPATFTEPAGLPPWVF
PCLVARVPAWEVVSFPSASGISFQAPSQAPEAR
EGGEEGGRLV*GLPGPAERG*WTGPPGAAAPAPC
HCSWPLPWV**SFTVWWQMGIPPEVLKLMASSV
ETLRKTVQLPPHNQSGKATSLGAPSNTSITASK
GDAASWWPSRRPPVSVMKATLEQGVRELTCFT*E
ETEDRFW*FV**QLW*FLLFWSSVSAHAVTLFG
NVVKERRKKKKWKLWVKI*LLSMKILKRQILLK
RL*SYLQVGGKLQSALLI*KWTECVSGKQLVDMN
FK*CIYFLFATLVCVIIF**EH*LYVYCLVIGK
KATG*VATTEGKFQ*PFT*VLSPGLLVKQKRKV
ERRLGLRN*INNKATIYQAFTMC**V*NILFPLF
LSAIHEIAIIILISYIWKQGQRSQVK*SNPDLE
SWRLVETD*KSRFI*IKIYALNHHFC*SESVQP
NSII*PKVLY*TVFWYDCEMSRPSSHRHTE*EIL
AYIAVTIMLSIELAFIMFLCSFINSQ*CDYIFT
LLKQIDWPHN*GNE*RWKSKCLAFLKPWVSVYA
Q*NINNITERGCLYGSNGQ*QHWRDQVGTGCDHI
LIYVLDDSIYDCSQVNFDAVNS*P*HLLLGTIL
PSAPLLHEKTML*CFFFPGLSLADQQNLSSHFV
LILKYQWWLSKFGRSDIKKEFQRFLYLFKMYFIV
ALHTLNNFVYVNCQYYHVIK*IYF*NKVNVWLL
GNFLFYHELLMKINLNTIAFKHLMLCKPYPLVY
LKESILTMCFL*ELKHLGVCWTSMGLSCQYPGHI
FELHLQLHLIGLRWALNKLG

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