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Sequences

Sequence C11ORF4.gAug05

TCTCCTCCACTAGCTCTGAGTGGGGCCACAGGACCACTACGCTTGCCGGACACAAGGCCCCACAGGTGGAAGAGGGAGTCTTGCTTTGTCACCCAGGCTG GAGTACAGTGATGTGATCTTGGCTCACTGCAACCTCTGCTCACCAGGCCTCCCTCCCTGACATGGAGAGTAACCTGTCTGGCCTGGTGCCTGCTGCCGGG CTGGTGCCTGCGCTGCCACCTGCTGTGACCCTGGGGCTGACAGCTGCCTACACCACCCTGTATGCCCTGCTCTTCTTCTCCGTCTATGCCCAGCTCTGGC TGGTGCTTCTGTATGGGCACAAGCGTCTCAGCTATCAGACGGTGTTCCTGGCCCTCTGTCTGCTCTGGGCCGCCTTGCGTACCACCCTCTTCTCCTTCTA CTTCCGAGATACTCCCCGCGCCAACCGCCTGGGGCCCTTGCCCTTCTGGCTTCTCTACTGCTGCCCCGTCTGCCTGCAGTTCTTCACCTTGACGCTTATG AACCTCTACTTTGCCCAGGTGGTGTTCAAGGCCAAGGTGAAGCGTCGGCCGGAGATGAGCCGAGGCTTCGGTTCTCAGGGTGTAGAGGAAGCTGGGAGCC TTCTCTCCCTGAGGGTCCTCTGTTGTTACCTGTGCCAGGGGACCAGTGTGTGCCAGGCGGCCGC

C11ORF4.F1 (forward primer)
C11ORF4.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

SPPLALSGATGPLRLPDTRPHRWKRESCFVTQA GVQ*CDLGSLQPLLTRPPSLTWRVTCLAWCLLPG WCLRCHLL*PWG*QLPTPPCMPCSSSPSMPSSG WCFCMGTSVSAIRRCSWPSVCSGPPCVPPSSPS TSEILPAPTAWGPCPSGFSTAAPSACSSSP*RL* TSTLPRWCSRPR*SVGRR*AEASVLRV*RKLGA FSP*GSSVVTCARGPVCARRP

Frame 1

LLH*L*VGPQDHYACRTQGPTGGRGSLALSPRL EYSDVILAHCNLCSPGLPP*HGE*PVWPGACCR AGACAATCCDPGADSCLHHPVCPALLLRLCPALA GASVWAQASQLSDGVPGPLSALGRLAYHPLLLL LPRYSPRQPPGALALLASLLLPRLPAVLHLDAY EPLLCPGGVQGQGEASAGDEPRLRFSGCRGSWEP SLPEGPLLLPVPGDQCVPGGR

Frame 2

SSTSSEWGHRTTTLAGHKAPQVEEGVLLCHPGW STVM*SWLTATSAHQASLPDMESNLSGLVPAAG LVPALPPAVTLGLTAAYTTLYALLFFSVYAQLW LVLLYGHKRLSYQTVFLALCLLWAALRTTLFSFY FRDTPRANRLGPLPFWLLYCCPVCLQFFTLTLM NLYFAQVVFKAKVKRRPEMSRGFGSQGVEEAGS LLSLRVLCCYLCQGTSVCQAA

Sequence C11ORF4.fAug05

CAAGGCTCCTCGTCCGCATCCTTTTTCCTCCCTCCTTCGGCCCCGGGGCTCACATCCTCGCTGCCTCACACCTTGGGCGTGTGTGGGAGCAGGAGAGGGA TTCTCTGGGGTCCCCATTTGGAACCTGGAGACCCACTTCTGCCAACAAGTCCTTCAGCCCCTCTGGGCCTTAGTTTCCCCCCAGTGAAGTGGGGATAGTG ACAGCTCCCCCGGGAGCCAGTGAAAACTTTGTGAAGGGAGTGATTACTGCGGGCGAAGGCGCTTGGCACAGCCACAACTGAAGCTCAGCCGTCGGATGTG ATTATTGTTGCTATTGTTACCGCTCCGCCTGGACTGGGTGCCCGGTCGGAAAGTCCCGCTCCCCGCTCCTCCTCCCAGGCGCGGAAGCTTCGCGCCGCCC GCCTCGGGGGACACCTCCCGCGCCATCTCGGGGGCACTGGAGATAAGGAAAGGAACCTCCTCCCTGGCTGGGATGGCTGCCCGGGCGCGGGAGGAGGGCC GGGGACCCGGTGAGGGAGGACCGTAGGGCCCGTGGGGGCTTCACGCCTGGCAGGCGGCCGGCCAGGCCTCCCTCCCTGACATGGAGAGTAACCTGTCTGG CCTGGTGCCTGCTGCCGGGCTGGTGCCTGCGCTGCCACCTGCTGTGACCCTGGGGCTGACAGCTGCCTACACCACCCTGTATGCCCTGCTCTTCTTCTCC GTCTATGCCCAGCTCTGGCTGGTGCTTCTGTATGGGCACAAGCGTCTCAGCTATCAGACGGTGTTCCTGGCCCTCTGTCTGCTCTGGGCCGCCTTGCGTA CCACCCTCTTCTCCTTCTACTTCCGAGATACTCCCCGCGCCAACCGCCTGGGGCCCTTGCCCTTCTGGCTTCTCTACTGCTGCCCCGTCTGCCTGCAGTT CTTCACCTTGACGCTTATGAACCTCTACTTTGCCCAGGTGGTGTTCAAGGCCAAGGTGAAGCGTCGGCCGGAGATGAGCCGAGGCTTGCTCGCTGTCCGA GGGGCCTTTGTGGGGGCCTCGCTGCTCTTTCTGCTGGTGAACGTGCTGTGTGCTGTGCTCTCCCATCGGCGCCGGGCACAGCCCTGGGCCCTGCTGCTTG TCCGCGTCCTGGTGAGCGACTCCCTGTTCGTCATCTGCGCGCTGTCTCTTGCTGCCTGCCTCTGCCTCGTCGCCAGGCGGGCGCCCTCCACTAGCATCTA CCTGGAGGCCAAGGGGACCAGTGTGTGCCAGGCGGCCGC

C11ORF4.F1 (forward primer)
C11ORF4.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

QGSSSASFFLPPSAPGLTSSLPHTLGVCGSRRG ILWGPHLEPGDPLLPTSPSAPLGLSFPPVKWG** QLPREPVKTL*RE*LLRAKALGTATTEAQPSDV IIVAIVTAPPGLGARSESPAPRSSSQARKLRAA RLGGHLPRHLGGTGDKERNLLPGWDGCPGAGGGP GTR*GRTVGPVGASRLAGGRPGLPP*HGE*PVW PGACCRAGACAATCCDPGADSCLHHPVCPALLL RLCPALAGASVWAQASQLSDGVPGPLSALGRLAY HPLLLLLPRYSPRQPPGALALLASLLLPRLPAV LHLDAYEPLLCPGGVQGQGEASAGDEPRLARCP RGLCGGLAALSAGERAVCCALPSAPGTALGPAAC PRPGERLPVRHLRAVSCCLPLPRRQAGALH*HL PGGQGDQCVPGGR

Frame 1

KAPRPHPFSSLLRPRGSHPRCLTPWACVGAGEG FSGVPIWNLETHFCQQVLQPLWALVSPQ*SGDS DSSPGSQ*KLCEGSDYCGRRRLAQPQLKLSRRM* LLLLLLPLRLDWVPGRKVPLPAPPPRRGSFAPP ASGDTSRAISGALEIRKGTSSLAGMAARAREEG RGPGEGGP*GPWGLHAWQAAGQASLPDMESNLSG LVPAAGLVPALPPAVTLGLTAAYTTLYALLFFS VYAQLWLVLLYGHKRLSYQTVFLALCLLWAALR TTLFSFYFRDTPRANRLGPLPFWLLYCCPVCLQF FTLTLMNLYFAQVVFKAKVKRRPEMSRGLLAVR GAFVGASLLFLLVNVLCAVLSHRRRAQPWALLL VRVLVSDSLFVICALSLAACLCLVARRAPSTSIY LEAKGTSVCQAA

Frame 2

RLLVRILFPPSFGPGAHILAASHLGRVWEQERD SLGSPFGTWRPTSANKSFSPSGP*FPPSEVGIV TAPPGASENFVKGVITAGEGAWHSHN*SSAVGC DYCCYCYRSAWTGCPVGKSRSPLLLPGAEASRRP PRGTPPAPSRGHWR*GKEPPPWLGWLPGRGRRA GDPVREDRRARGGFTPGRRPARPPSLTWRVTCL AWCLLPGWCLRCHLL*PWG*QLPTPPCMPCSSSP SMPSSGWCFCMGTSVSAIRRCSWPSVCSGPPCV PPSSPSTSEILPAPTAWGPCPSGFSTAAPSACS SSP*RL*TSTLPRWCSRPR*SVGRR*AEACSLSE GPLWGPRCSFCW*TCCVLCSPIGAGHSPGPCCL SASW*ATPCSSSARCLLLPASASSPGGRPPLAS TWRPRGPVCARRP

Sequence C11ORF4.dAug05

GCTTTATTAACATTTGGTAGTGAGCACGGCCCCCAGGGCATCGCGGGGGCTCGGGTCCCGGTGACGCAACGGTTAAACCTGGCTCGCGACTTAGCGCAGG CGCCTGGGGGAAAGCCCGGAGCCTGAGGGCGGGGCCACGGAGCCACTTCCGGCGGCTGTGGGCGGAAAACCCAAAACTTCCGATGGGACCAAGCCTTCCG TGGCTTCACACGCACCGGAAGGGAATCTGGGTCAGCCCTCCCTCCAAAGGAGACAGCACGGATCCTCTTTTTGCATAGGCCTGAGGGAAGTACTTCCGCC CATATTCAAGATGGCTGCCCAGGGCAGTGGGAACGGGTGGAGTTTCGGGATGTGGAGCGAAGGTCACTGGGAGGGGGCGGAGCTTCCCCTGCCCAAGTTC CGATCCCACCAGGACTGGAAGACTCGCGTCCAGCTGGAGCTTTGCCGCATCTGCGTTGCTGTGCCCGCGCTCTTCGGGCGAGGAAGTCCCTTCTGGTGGA GGGAGAAAAGGGTTACATGAGTTAGATTACCATGGCAGCTCGAGCCAGGCTCTCCACTTCCGTGAGCCTTCCTCTAAGTGAGATCGGGGGGGAGATAATG AAGGCCACAACTCCCAGGAGGCTCCACGCCGGGCGGTCAGGGACGCTCGCGAGGACGCATGGGCCCCCAGGAATGGGAACTGGTTAGATCCCAGGAACTC CGTGCCGGAGACGGGAAGAGAGGATGCCTAGGGCCCTAAATGGAAAGGCCAGAGCTGGAGACCTAGCACCCAGAAGATGAGACCGCAATTCGAGAATCAA AGGGCCGGGAGCGATGGCCTCCCTCCCTGACATGGAGAGTAACCTGTCTGGCCTGGTGCCTGCTGCCGGGCTGGTGCCTGCGCTGCCACCTGCTGTGACC CTGGGGCTGACAGCTGCCTACACCACCCTGTATGCCCTGCTCTTCTTCTCCGTCTATGCCCAGCTCTGGCTGGTGCTTCTGTATGGGCACAAGCGTCTCA GCTATCAGACGGTGTTCCTGGCCCTCTGTCTGCTCTGGGCCGCCTTGCGTACCACCCTCTTCTCCTTCTACTTCCGAGATACTCCCCGCGCCAACCGCCT GGGGCCCTTGCCCTTCTGGCTTCTCTACTGCTGCCCCGTCTGCCTGCAGTTCTTCACCTTGACGCTTATGAACCTCTACTTTGCCCAGGTGGTGTTCAAG GCCAAGGTGAAGCGTCGGCCGGAGATGAGCCGAGGCTTGCTCGCTGTCCGAGGGGCCTTTGTGGGGGCCTCGCTGCTCTTTCTGCTGGTGAACGTGCTGT GTGCTGTGCTCTCCCATCGGCGCCGGGCACAGCCCTGGGCCCTGCTGCTTGTCCGCGTCCTGGTGAGCGACTCCCTGTTCGTCATCTGCGCGCTGTCTCT TGCTGCCTGCCTCTGCCTCGTCGCCAGGCGGGCGCCCTCCACTAGCATCTACCTGGAGGCCAAGGGGACCAGTGTGTGCCAGGCGGCCGCGATGGGTGGC GCCATGGTCCTGCTCTATGCCAGCCGGGCCTGCTACAACCTGACAGCACTGGCCTTGGCCCCCCAGAGCCGGCTGGACACCTTCGATTACGACTGGTACA ATGTGTCTGACCAGGTGGGCATACGCATGTCTGCCACCTCCTTAGTAGCCGTGGCACCTTGGCCCCAGCTGGCCTGGGCCCTGTCCCACTACCCCTTTGG CGTGACTGCAGACTGGCACCAGCCCCTGCCTTCCCACAGGCGGACCTGGTGAATGACCTGGGGAACAAAGGCTACCTGGTATTTGGCCTCATCCTCTTCG TGTGGGAGCTACTGCCCACCACCCTGCTGGTGGGCTTCTTCCGGGTGCACCGGCCCCCACAGGACCTGAGCACCAGCCACATCCTCAATGGGCAGGTCTT TGCCTCTCGGTCCTACTTCTTTGACCGGGCTGGGCACTGTGAAGATGAGGGCTGCTCCTGGGAGCACAGCCGGGGTGAGAGCACCAGTATGTCGGGCAGT CTAGGCTCTGGGAGCTGGTATGGTGCCATCGGGCGTGAGCCGGGCTGGTATGGGGGCAGCCAGACGAAGACCACTCCTCTGCTCTTCTCCCAGGTGCCAG GACCAGGCGGCCACCACCACAGTCTCTACTCCACCCCACAGACGTGATCCCCCTCCCTCCCCCACAGAATACCCAGGCCCCAGTCCCCCTCACCCTAGGC CCCTGTGCCAAGTTTGTCTGCCGCTTCTTGCCCAGGATCCTGGGGGTCGTGGCTACCCCCTCCTCTGGCCGGCTCCTTGCTGCTCCTGTCATAGTGAGCT TGTGCCGTCCCCCTAGGATGGGGGGCATGGCCCTGGCTGCCAGATGCCCACAGCACCCTGGCATGACCTGCCACCTCTGCTTCCACACCGGAGCCAGCTA CCTCTCCTGTGCCTGCCACTCAATAAACAGTGTCTGCGCCCCACAGTTGTG

C11ORF4.F1 (forward primer)
C11ORF4.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

ALLTFGSEHGPQGIAGARVPVTQRLNLARDLAQ APGGKPGA*GRGHGATSGGCGRKTQNFRWDQAFR GFTRTGRESGSALPPKETARILFLHRPEGSTSA HIQDGCPGQWERVEFRDVERRSLGGGGASPAQV PIPPGLEDSRPAGALPHLRCCARALRARKSLLVE GEKGYMS*ITMAARARLSTSVSLPLSEIGGEIM KATTPRRLHAGRSGTLARTHGPPGMGTG*IPGT PCRRREERMPRALNGKARAGDLAPRR*DRNSRIK GPGAMASLPDMESNLSGLVPAAGLVPALPPAVT LGLTAAYTTLYALLFFSVYAQLWLVLLYGHKRL SYQTVFLALCLLWAALRTTLFSFYFRDTPRANRL GPLPFWLLYCCPVCLQFFTLTLMNLYFAQVVFK AKVKRRPEMSRGLLAVRGAFVGASLLFLLVNVL CAVLSHRRRAQPWALLLVRVLVSDSLFVICALSL AACLCLVARRAPSTSIYLEAKGTSVCQAAAMGG AMVLLYASRACYNLTALALAPQSRLDTFDYDWY NVSDQVGIRMSATSLVAVAPWPQLAWALSHYPFG VTADWHQPLPSHRRTW*MTWGTKATWYLASSSS CGSYCPPPCWWASSGCTGPHRT*APATSSMGRS LPLGPTSLTGLGTVKMRAAPGSTAGVRAPVCRAV *ALGAGMVPSGVSRAGMGAARRRPLLCSSPRCQ DQAATTTVSTPPHRRDPPPSPTEYPGPSPPHPR PLCQVCLPLLAQDPGGRGYPLLWPAPCCSCHSEL VPSP*DGGHGPGCQMPTAPWHDLPPLLPHRSQL PLLCLPLNKQCLRPTVV

Frame 1

LY*HLVVSTAPRASRGLGSR*RNG*TWLAT*RR RLGESPEPEGGATEPLPAAVGGKPKTSDGTKPS VASHAPEGNLGQPSLQRRQHGSSFCIGLREVLPP IFKMAAQGSGNGWSFGMWSEGHWEGAELPLPKF RSHQDWKTRVQLELCRICVAVPALFGRGSPFWW REKRVT*VRLPWQLEPGSPLP*AFL*VRSGGR** RPQLPGGSTPGGQGRSRGRMGPQEWELVRSQEL RAGDGKRGCLGP*MERPELET*HPEDETAIRES KGRERWPPSLTWRVTCLAWCLLPGWCLRCHLL*P WG*QLPTPPCMPCSSSPSMPSSGWCFCMGTSVS AIRRCSWPSVCSGPPCVPPSSPSTSEILPAPTA WGPCPSGFSTAAPSACSSSP*RL*TSTLPRWCSR PR*SVGRR*AEACSLSEGPLWGPRCSFCW*TCC VLCSPIGAGHSPGPCCLSASW*ATPCSSSARCL LLPASASSPGGRPPLASTWRPRGPVCARRPRWVA PWSCSMPAGPATT*QHWPWPPRAGWTPSITTGT MCLTRWAYACLPPP**PWHLGPSWPGPCPTTPL A*LQTGTSPCLPTGGPGE*PGEQRLPGIWPHPLR VGATAHHPAGGLLPGAPAPTGPEHQPHPQWAGL CLSVLLL*PGWAL*R*GLLLGAQPG*EHQYVGQ SRLWELVWCHRA*AGLVWGQPDEDHSSALLPGAR TRRPPPQSLLHPTDVIPLPPPQNTQAPVPLTLG PCAKFVCRFLPRILGVVATPSSGRLLAAPVIVS LCRPPRMGGMALAARCPQHPGMTCHLCFHTGASY LSCACHSINSVCAPQL

Frame 2

FINIW**ARPPGHRGGSGPGDATVKPGSRLSAG AWGKARSLRAGPRSHFRRLWAENPKLPMGPSLP WLHTHRKGIWVSPPSKGDSTDPLFA*A*GKYFR PYSRWLPRAVGTGGVSGCGAKVTGRGRSFPCPSS DPTRTGRLASSWSFAASALLCPRSSGEEVPSGG GRKGLHELDYHGSSSQALHFREPSSK*DRGGDN EGHNSQEAPRRAVRDAREDAWAPRNGNWLDPRNS VPETGREDA*GPKWKGQSWRPSTQKMRPQFENQ RAGSDGLPP*HGE*PVWPGACCRAGACAATCCD PGADSCLHHPVCPALLLRLCPALAGASVWAQASQ LSDGVPGPLSALGRLAYHPLLLLLPRYSPRQPP GALALLASLLLPRLPAVLHLDAYEPLLCPGGVQ GQGEASAGDEPRLARCPRGLCGGLAALSAGERAV CCALPSAPGTALGPAACPRPGERLPVRHLRAVS CCLPLPRRQAGALH*HLPGGQGDQCVPGGRDGW RHGPALCQPGLLQPDSTGLGPPEPAGHLRLRLVQ CV*PGGHTHVCHLLSSRGTLAPAGLGPVPLPLW RDCRLAPAPAFPQADLVNDLGNKGYLVFGLILF VWELLPTTLLVGFFRVHRPPQDLSTSHILNGQVF ASRSYFFDRAGHCEDEGCSWEHSRGESTSMSGS LGSGSWYGAIGREPGWYGGSQTKTTPLLFSQVP GPGGHHHSLYSTPQT*SPSLPHRIPRPQSPSP*A PVPSLSAASCPGSWGSWLPPPLAGSLLLLS**A CAVPLGWGAWPWLPDAHSTLA*PATSASTPEPA TSPVPATQ*TVSAPHSC

Sequence C11ORF4.cAug05

GGCTATGGGCGGAAGTTTTCTTCAGAGACCGAGGGACGGGAATCCGTTGCCGCCCCCGGCCGGGCGGCACGACCCCGATTTGGGTATAGGAGAGAGGAGC GTGGGAACTGGCGGACCCCCATACAAGCCTCCCTCCCTGACATGGAGAGTAACCTGTCTGGCCTGGTGCCTGCTGCCGGGCTGGTGCCTGCGCTGCCACC TGCTGTGACCCTGGGGCTGACAGCTGCCTACACCACCCTGTATGCCCTGCTCTTCTTCTCCGTCTATGCCCAGCTCTGGCTGGTGCTTCTGTATGGGCAC AAGCGTCTCAGCTATCAGACGGTGTTCCTGGCCCTCTGTCTGCTCTGGGCCGCCTTGCGTACCACCCTCTTCTCCTTCTACTTCCGAGATACTCCCCGCG CCAACCGCCTGGGGCCCTTGCCCTTCTGGCTTCTCTACTGCTGCCCCGTCTGCCTGCAGTTCTTCACCTTGACGCTTATGAACCTCTACTTTGCCCAGGT GGTGTTCAAGGCCAAGGTGAAGCGTCGGCCGGAGATGAGCCGAGGCTTGCTCGCTGTCCGAGGGGCCTTTGTGGGGGCCTCGCTGCTCTTTCTGCTGGTG AACGTGCTGTGTGCTGTGCTCTCCCATCGGCGCCGGGCACAGCCCTGGGCCCTGCTGCTTGTCCGCGTCCTGGTGAGCGACTCCCTGTTCGTCATCTGCG CGCTGTCTCTTGCTGCCTGCCTCTGCCTCGTCGCCAGGCGGGCGCCCTCCACTAGCATCTACCTGGAGGCCAAGGGGACCAGTGTGTGCCAGGCGGCCGC GATGGGTGGCGCCATGGTCCTGCTCTATGCCAGCCGGGCCTGCTACAACCTGACAGCACTGGCCTTGGCCCCCCAGAGCCGGCTGGACACCTTCGATTAC GACTGGTACAATGTGTCTGACCAGGCGGACCTGGTGAATGACCTGGGGAACAAAGGCTACCTGGTATTTGGCCTCATCCTCTTCGTGTGGGAGCTACTGC CCACCACCCTGCTGGTGGGCTTCTTCCGGGTGCACCGGCCCCCACAGGACCTGTATGTCGGGCAGTCTAGGCTCTGGGAGCTGGTATGGTGCCATCGGGC GTGAGCCGGGCTGGTATGGGGGCAGCCAGACGAAGACCACTCCTCTGCTCTTCTCCCAGGTGCCAGGACCAGGCGGCCACCACCACAGTCTCTACTCCAC CCCACAGACGTGATCCCCCTCCCTCCCCCACAGAATACCCAGGCCCCAGTCCCCCTCACCCTAGGCCCCTGTGCCAAGTTTGTCTGCCGCTTCTTGCCCA GGATCCTGGGGGTCGTGGCTACCCCCTCCTCTGGCCGGCTCCTTGCTGCTCCTGTCATAGTGAGCTTGTGCCGTCCCCCTAGGATGGGGGGCATGGCCCT GGCTGCCAGATGCCCACAGCACCCTGGCATGACCTGCCACCTCTGCTTCCACACCGGAGCCAGCTACCTCTCCTGTGCCTGCCACTCAATAAACAGTGTC TGCGCCCCACAGTTGTG

C11ORF4.F1 (forward primer)
C11ORF4.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

GYGRKFSSETEGRESVAAPGRAARPRFGYRREE RGNWRTPIQASLPDMESNLSGLVPAAGLVPALPP AVTLGLTAAYTTLYALLFFSVYAQLWLVLLYGH KRLSYQTVFLALCLLWAALRTTLFSFYFRDTPR ANRLGPLPFWLLYCCPVCLQFFTLTLMNLYFAQV VFKAKVKRRPEMSRGLLAVRGAFVGASLLFLLV NVLCAVLSHRRRAQPWALLLVRVLVSDSLFVIC ALSLAACLCLVARRAPSTSIYLEAKGTSVCQAAA MGGAMVLLYASRACYNLTALALAPQSRLDTFDY DWYNVSDQADLVNDLGNKGYLVFGLILFVWELL PTTLLVGFFRVHRPPQDLYVGQSRLWELVWCHRA *AGLVWGQPDEDHSSALLPGARTRRPPPQSLLH PTDVIPLPPPQNTQAPVPLTLGPCAKFVCRFLP RILGVVATPSSGRLLAAPVIVSLCRPPRMGGMAL AARCPQHPGMTCHLCFHTGASYLSCACHSINSV CAPQL

Frame 1

AMGGSFLQRPRDGNPLPPPAGRHDPDLGIGERS VGTGGPPYKPPSLTWRVTCLAWCLLPGWCLRCH LL*PWG*QLPTPPCMPCSSSPSMPSSGWCFCMGT SVSAIRRCSWPSVCSGPPCVPPSSPSTSEILPA PTAWGPCPSGFSTAAPSACSSSP*RL*TSTLPR WCSRPR*SVGRR*AEACSLSEGPLWGPRCSFCW* TCCVLCSPIGAGHSPGPCCLSASW*ATPCSSSA RCLLLPASASSPGGRPPLASTWRPRGPVCARRP RWVAPWSCSMPAGPATT*QHWPWPPRAGWTPSIT TGTMCLTRRTW*MTWGTKATWYLASSSSCGSYC PPPCWWASSGCTGPHRTCMSGSLGSGSWYGAIG REPGWYGGSQTKTTPLLFSQVPGPGGHHHSLYST PQT*SPSLPHRIPRPQSPSP*APVPSLSAASCP GSWGSWLPPPLAGSLLLLS**ACAVPLGWGAWP WLPDAHSTLA*PATSASTPEPATSPVPATQ*TVS APHSC

Frame 2

LWAEVFFRDRGTGIRCRPRPGGTTPIWV*ERGA WELADPHTSLPP*HGE*PVWPGACCRAGACAAT CCDPGADSCLHHPVCPALLLRLCPALAGASVWA QASQLSDGVPGPLSALGRLAYHPLLLLLPRYSPR QPPGALALLASLLLPRLPAVLHLDAYEPLLCPG GVQGQGEASAGDEPRLARCPRGLCGGLAALSAG ERAVCCALPSAPGTALGPAACPRPGERLPVRHLR AVSCCLPLPRRQAGALH*HLPGGQGDQCVPGGR DGWRHGPALCQPGLLQPDSTGLGPPEPAGHLRL RLVQCV*PGGPGE*PGEQRLPGIWPHPLRVGATA HHPAGGLLPGAPAPTGPVCRAV*ALGAGMVPSG VSRAGMGAARRRPLLCSSPRCQDQAATTTVSTP PHRRDPPPSPTEYPGPSPPHPRPLCQVCLPLLAQ DPGGRGYPLLWPAPCCSCHSELVPSP*DGGHGP GCQMPTAPWHDLPPLLPHRSQLPLLCLPLNKQC LRPTVV

Sequence C11ORF4.aAug05

GGCTATGGGCGGAAGTTTTCTTCAGAGACCGAGGGACGGGAATCCGTTGCCGCCCCCGGCCGGGCGGCACGACCCCGATTTGGGTATAGGAGAGAGGAGC GTGGGAACTGGCGGACCCCCATACAAGTAAAGGCGGGCGGCACGGGGCACGGCCCACCCGGGGACCCCCTGGATGCGGCACGGCCCCCGCAACTCGGCCG CCCTCCTGCCGCCTCCCGTAACCCCGGGCGCCCGCCAGCGGCGCAGCTCGCGCCAATTCTCGTACGGTTTCACGCCGGGTGCGGCGCTGGACTGCGGAGC CAGCCGGGCCGCTGCGCAATTTGCGTAGCGAACGGCGCCCGCGCAGGCGCGGAGGGGGAGGGGGGCGGAGCAGCGGGAGCCGGGGAGCCGGAGCCCCGGG TCCCCACGACCTGAGCCGGCTCTCCCATCAGCGGCCTGAGGACCTGGCGTCCGCCTCCTCCCTCCCCTTGAGGCTGGAGCGTGGACGCGGTGGGGGAGGG TGGGGCGGGGGCAGCTTTCGAGAATTACGAGGACAGGAGGCAGAGGCCCTCCCCATCCGCATTATTGGAGGAGAGAGAGCCTCCCCCAGCTTGGGGAGGG GGAGAGCGGGGCATTGGGCGCCCCTCGCAGCGGCCGCTGCCCTGACCCGACGGGTATCAGCCGGCTCTCCCCCTCCACCCAGGACGACATGAACGACCGA GGCCAGGGAGTCCTCTCCTTGGGCCTCTGCATCCCCCCATCCTTGGCTCTGGGGTAGGCCCAGGGAGGAGACACCCCCAACCCCTATCCGGTCTGTCCTG GAGAAAAGAGACTGCCCTTCCATGCCCCTGAGTGAGGGGCCTGGGGCCCAGGCTGCCTGTGTTCCCCAAGGGCAAGGGTCTCTCTGTTGAGGAGGAGGGG CCTGTCAGCCACAACTTCTTTCCTCCTGAGCGCCCCATCTCCCTCTCTGCACCCTGCAATTCCCACCCCTCCGTATTTATTTCCCTGGTCCCGCCGACAG TCCCTCCTTGTCTGTCTCCGGGATTCAGGCCTCCCTCCCTGACATGGAGAGTAACCTGTCTGGCCTGGTGCCTGCTGCCGGGCTGGTGCCTGCGCTGCCA CCTGCTGTGACCCTGGGGCTGACAGCTGCCTACACCACCCTGTATGCCCTGCTCTTCTTCTCCGTCTATGCCCAGCTCTGGCTGGTGCTTCTGTATGGGC ACAAGCGTCTCAGCTATCAGACGGTGTTCCTGGCCCTCTGTCTGCTCTGGGCCGCCTTGCGTACCACCCTCTTCTCCTTCTACTTCCGAGATACTCCCCG CGCCAACCGCCTGGGGCCCTTGCCCTTCTGGCTTCTCTACTGCTGCCCCGTCTGCCTGCAGTTCTTCACCTTGACGCTTATGAACCTCTACTTTGCCCAG GTGGTGTTCAAGGCCAAGGTGAAGCGTCGGCCGGAGATGAGCCGAGGCTTGCTCGCTGTCCGAGGGGCCTTTGTGGGGGCCTCGCTGCTCTTTCTGCTGG TGAACGTGCTGTGTGCTGTGCTCTCCCATCGGCGCCGGGCACAGCCCTGGGCCCTGCTGCTTGTCCGCGTCCTGGTGAGCGACTCCCTGTTCGTCATCTG CGCGCTGTCTCTTGCTGCCTGCCTCTGCCTCGTCGCCAGGCGGGCGCCCTCCACTAGCATCTACCTGGAGGCCAAGGGGACCAGTGTGTGCCAGGCGGCC GCGATGGGTGGCGCCATGGTCCTGCTCTATGCCAGCCGGGCCTGCTACAACCTGACAGCACTGGCCTTGGCCCCCCAGAGCCGGCTGGACACCTTCGATT ACGACTGGTACAATGTGTCTGACCAGGCGGACCTGGTGAATGACCTGGGGAACAAAGGCTACCTGGTATTTGGCCTCATCCTCTTCGTGTGGGAGCTACT GCCCACCACCCTGCTGGTGGGCTTCTTCCGGGTGCACCGGCCCCCACAGGACCTGAGCACCAGCCACATCCTCAATGGGCAGGTCTTTGCCTCTCGGTCC TACTTCTTTGACCGGGCTGGGCACTGTGAAGATGAGGGCTGCTCCTGGGAGCACAGCCGGGGTGAGAGCACCAGTATGTCGGGCAGTCTAGGCTCTGGGA GCTGGTATGGTGCCATCGGGCGTGAGCCGGGCTGGTATGGGGGCAGCCAGACGAAGACCACTCCTCTGCTCTTCTCCCAGGTGCCAGGACCAGGCGGCCA CCACCACAGTCTCTACTCCACCCCACAGACGTGATCCCCCTCCCTCCCCCACAGAATACCCAGGCCCCAGTCCCCCTCACCCTAGGCCCCTGTGCCAAGT TTGTCTGCCGCTTCTTGCCCAGGATCCTGGGGGTCGTGGCTACCCCCTCCTCTGGCCGGCTCCTTGCTGCTCCTGTCATAGTGAGCTTGTGCCGTCCCCC TAGGATGGGGGGCATGGCCCTGGCTGCCAGATGCCCACAGCACCCTGGCATGACCTGCCACCTCTGCTTCCACACCGGAGCCAGCTACCTCTCCTGTGCC TGCCACTCAATAAACAGTGTCTGCGCCCCACAGTTGTG

C11ORF4.F1 (forward primer)
C11ORF4.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

GYGRKFSSETEGRESVAAPGRAARPRFGYRREE RGNWRTPIQVKAGGTGHGPPGDPLDAARPPQLGR PPAASRNPGRPPAAQLAPILVRFHAGCGAGLRS QPGRCAICVANGARAGAEGEGGGAAGAGEPEPR VPTT*AGSPISGLRTWRPPPPSP*GWSVDAVGEG GAGAAFENYEDRRQRPSPSALLEEREPPPAWGG GERGIGRPSQRPLP*PDGYQPALPLHPGRHERP RPGSPLLGPLHPPILGSGVGPGRRHPQPLSGLSW RKETALPCP*VRGLGPRLPVFPKGKGLSVEEEG PVSHNFFPPERPISLSAPCNSHPSVFISLVPPT VPPCLSPGFRPPSLTWRVTCLAWCLLPGWCLRCH LL*PWG*QLPTPPCMPCSSSPSMPSSGWCFCMG TSVSAIRRCSWPSVCSGPPCVPPSSPSTSEILP APTAWGPCPSGFSTAAPSACSSSP*RL*TSTLPR WCSRPR*SVGRR*AEACSLSEGPLWGPRCSFCW *TCCVLCSPIGAGHSPGPCCLSASW*ATPCSSS ARCLLLPASASSPGGRPPLASTWRPRGPVCARRP RWVAPWSCSMPAGPATT*QHWPWPPRAGWTPSI TTGTMCLTRRTW*MTWGTKATWYLASSSSCGSY CPPPCWWASSGCTGPHRT*APATSSMGRSLPLGP TSLTGLGTVKMRAAPGSTAGVRAPVCRAV*ALG AGMVPSGVSRAGMGAARRRPLLCSSPRCQDQAA TTTVSTPPHRRDPPPSPTEYPGPSPPHPRPLCQV CLPLLAQDPGGRGYPLLWPAPCCSCHSELVPSP *DGGHGPGCQMPTAPWHDLPPLLPHRSQLPLLC LPLNKQCLRPTVV

Frame 1

AMGGSFLQRPRDGNPLPPPAGRHDPDLGIGERS VGTGGPPYK*RRAARGTAHPGTPWMRHGPRNSA ALLPPPVTPGARQRRSSRQFSYGFTPGAALDCGA SRAAAQFA*RTAPAQARRGRGAEQREPGSRSPG SPRPEPALPSAA*GPGVRLLPPLEAGAWTRWGR VGRGQLSRITRTGGRGPPHPHYWRRESLPQLGEG ESGALGAPRSGRCPDPTGISRLSPSTQDDMNDR GQGVLSLGLCIPPSLALG*AQGGDTPNPYPVCP GEKRLPFHAPE*GAWGPGCLCSPRARVSLLRRRG LSATTSFLLSAPSPSLHPAIPTPPYLFPWSRRQ SLLVCLRDSGLPP*HGE*PVWPGACCRAGACAA TCCDPGADSCLHHPVCPALLLRLCPALAGASVWA QASQLSDGVPGPLSALGRLAYHPLLLLLPRYSP RQPPGALALLASLLLPRLPAVLHLDAYEPLLCP GGVQGQGEASAGDEPRLARCPRGLCGGLAALSAG ERAVCCALPSAPGTALGPAACPRPGERLPVRHL RAVSCCLPLPRRQAGALH*HLPGGQGDQCVPGG RDGWRHGPALCQPGLLQPDSTGLGPPEPAGHLRL RLVQCV*PGGPGE*PGEQRLPGIWPHPLRVGAT AHHPAGGLLPGAPAPTGPEHQPHPQWAGLCLSV LLL*PGWAL*R*GLLLGAQPG*EHQYVGQSRLWE LVWCHRA*AGLVWGQPDEDHSSALLPGARTRRP PPQSLLHPTDVIPLPPPQNTQAPVPLTLGPCAK FVCRFLPRILGVVATPSSGRLLAAPVIVSLCRPP RMGGMALAARCPQHPGMTCHLCFHTGASYLSCA CHSINSVCAPQL

Frame 2

LWAEVFFRDRGTGIRCRPRPGGTTPIWV*ERGA WELADPHTSKGGRHGARPTRGPPGCGTAPATRP PSCRLP*PRAPASGAARANSRTVSRRVRRWTAE PAGPLRNLRSERRPRRRGGGGGRSSGSRGAGAPG PHDLSRLSHQRPEDLASASSLPLRLERGRGGGG WGGGSFRELRGQEAEALPIRIIGGERASPSLGR GRAGHWAPLAAAAALTRRVSAGSPPPPRTT*TTE ARESSPWASASPHPWLWGRPREETPPTPIRSVL EKRDCPSMPLSEGPGAQAACVPQGQGSLC*GGG ACQPQLLSS*APHLPLCTLQFPPLRIYFPGPADS PSLSVSGIQASLPDMESNLSGLVPAAGLVPALP PAVTLGLTAAYTTLYALLFFSVYAQLWLVLLYG HKRLSYQTVFLALCLLWAALRTTLFSFYFRDTPR ANRLGPLPFWLLYCCPVCLQFFTLTLMNLYFAQ VVFKAKVKRRPEMSRGLLAVRGAFVGASLLFLL VNVLCAVLSHRRRAQPWALLLVRVLVSDSLFVIC ALSLAACLCLVARRAPSTSIYLEAKGTSVCQAA AMGGAMVLLYASRACYNLTALALAPQSRLDTFD YDWYNVSDQADLVNDLGNKGYLVFGLILFVWELL PTTLLVGFFRVHRPPQDLSTSHILNGQVFASRS YFFDRAGHCEDEGCSWEHSRGESTSMSGSLGSG SWYGAIGREPGWYGGSQTKTTPLLFSQVPGPGGH HHSLYSTPQT*SPSLPHRIPRPQSPSP*APVPS LSAASCPGSWGSWLPPPLAGSLLLLS**ACAVP LGWGAWPWLPDAHSTLA*PATSASTPEPATSPVP ATQ*TVSAPHSC