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Sequences

Sequence ADAM15.fAug05

CTGGGGTGAGCCGCGCACTGCCTCGCCTGCAGTCGGGAAAGCCTGCCCGCCCGACCTCTCCGAGCCAGGCCGCGCACAGGAGGCAGGGAGGCCGCGAAGC TACTAGGGAGGGGTCCGGACCTGGCGCCGGGTGAAGGCGCGCCGCCCAAGCCGGTCGGACCGGGCACCGGCTCCCACTCCGCACAGTTGCGGGGAAGCGG TAGCGCTGAGCAGCGCGGGCGTAGTGGGCGGTGTCCCCGCTCCCGAGGCACCCGGCGCGCAGCGGGGCGGGCTTTGCCGGGGGCGGAGCTTGGCTTGGGG CCGGGTGGGAGGGGGCGGGCCGGGGCGGGGCCTGGTGGCCGCGCGGCGCTGCTGGGTTCTCCGAGGCGACCTGGCCGCCGGCCGCTCCTCCGCGCGCTGT TCCGCACTTGCTGCCCTCGCCCGGCCCGGAGCGCCGCTGCCATGCGGCTGGCGCTGCTCTGGGCCCTGGGGCTCCTGGGCGCGGGCAGCCCTCTGCCTTC CTGGCCGCTCCCAAATATAGGTGGCACTGAGGAGCAGCAGGCAGAGTCAGAGAAGGCCCCGAGGGAGCCCTTGGAGCCCCAGGTCCTTCAGGACGATCTC CCAATTAGCCTCAAAAAGGTGCTTCAGACCAGTCTGCCTGAGCCCCTGAGGATCAAGTTGGAGCTGGACGGTGACAGTCATATCCTGGAGCTGCTACAGA ATAGGGAGTTGGTCCCAGGCCGCCCAACCCTGGTGTGGTACCAGCCCGATGGCACTCGGGTGGTCAGTGAGGGACACACTTTGGAGAACTGCTGCTACCA GGGAAGAGTGCGGGGATATGCAGGCTCCTGGGTGTCCATCTGCACCTGCTCTGGGCTCAGAGGCTTGGTGGTCCTGACCCCAGAGAGAAGCTATACCCTG GAGCAGGGGCCTGGGGACCTTCAGGGTCCTCCCATTATTTCGCGAATCCAAGATCTCCACCTGCCAGGCCACACCTGTGCCCTGAGCTGGCGGGAATCTG TACACACTCAGAAGCCACCAGAGCACCCCCTGGGACAGCGCCACATTCGCCGGAGGCGGGATGTGGTAACAGAGACCAAGACTGTGGAGTTGGTGATTGT GGCTGATCACTCGGAGGCCCAGAAATACCGGGACTTCCAGCACCTGCTAAACCGCACACTGGAAGTGGCCCTCTTGCTGGACACATTCTTCCGGCCCCTG AATGTACGAGTGGCACTAGTGGGCCTGGAGGCCTGGACCCAGCGTGACCTGGTGGAGATCAGCCCAAACCCAGCTGTCACCCTCGAAAACTTCCTCCACT GGCGCAGGGCACATTTGCTGCCTCGATTGCCCCATGACAGTGCCCAGCTGGTGACTGGTACTTCATTCTCTGGGCCTACGGTGGGCATGGCCATTCAGAA CTCCATCTGTTCTCCTGACTTCTCAGGAGGTGTGAACATGGACCACTCCACCAGCATCCTGGGAGTCGCCTCCTCCATAGCCCATGAGTTGGGCCACAGC CTGGGCCTGGACCATGATTTGCCTGGGAATAGCTGCCCCTGTCCAGGTCCAGCCCCAGCCAAGACCTGCATCATGGAGGCCTCCACAGACTTCCTACCAG GCCTGAACTTCAGCAACTGCAGCCGACGGGCCCTGGAGAAAGCCCTCCTGGATGGAATGGGCAGCTGCCTCTTCGAACGGCTGCCTAGCCTACCCCCTAT GGCTGCTTTCTGCGGAAATATGTTTGTGGAGCCGGGCGAGCAGTGTGACTGTGGCTTCCTGGATGACTGCGTCGATCCCTGCTGTGATTCTTTGACCTGC CAGCTGAGGCCAGGTGCACAGTGTGCATCTGACGGACCCTGTTGTCAAAATTGCCAGCTGCGCCCGTCTGGCTGGCAGTGTCGTCCTACCAGAGGGGATT GTGACTTGCCTGAATTCTGCCCAGGAGACAGCTCCCAGTGTCCCCCTGATGTCAGCCTAGGGGATGGCGAGCCCTGCGCTGGCGGGCAAGCTGTGTGCAT GCACGGGCGTTGTGCCTCCTATGCCCAGCAGTGCCAGTCACTTTGGGGACCTGGAGCCCAGCCCGCTGCGCCACTTTGCCTCCAGACAGCTAATACTCGG GGAAATGCTTTTGGGAGCTGTGGGCGCAACCCCAGTGGCAGTTATGTGTCCTGCACCCCTAGAGATGCCATTTGTGGGCAGCTCCAGTGCCAGACAGGTA GGACCCAGCCTCTGCTGGGCTCCATCCGGGATCTACTCTGGGAGACAATAGATGTGAATGGGACTGAGCTGAACTGCAGCTGGGTGCACCTGGACCTGGG CAGTGATGTGGCCCAGCCCCTCCTGACTCTGCCTGGCACAGCCTGTGGCCCTGGCCTGGTGTGTATAGACCATCGATGCCAGCGTGTGGATCTCCTGGGG GCACAGGAATGTCGAAGCAAATGCCATGGACATGGGGTCTGTGACAGCAACAGGCACTGCTACTGTGAGGAGGGCTGGGCACCCCCTGACTGCACCACTC AGCTCAAAGCAACCAGCTCCCTGACCACAGGGCTGCTCCTCAGCCTCCTGGTCTTATTGGTCCTGGTGATGCTTGGTGCCAGCTACTGGTACCGTGCCCG CCTGCACCAGCGACTCTGCCAGCTCAAGGGACCCACCTGCCAGTACAGGGCAGCCCAATCTGGTCCCTCTGAACGGCCAGGACCTCCGCAGAGGGCCCTG CTGGCACGAGGCACTAAGCAGGCTAGTGCTCTCAGCTTCCCGGCCCCCCCTTCCAGGCCGCTGCCGCCTGACCCTGTGTCCAAGAGACTCCAGGCTGAGC TGGCTGACCGACCCAATCCCCCTACCCGCCCTCTGCCCGCTGACCCGGTGGTGAGAAGCCCGAAGGTAACGGTGGGGGGAGAGAAGGGCACGGCCTCTCC CCCCACCTAGGGCTGTGGTGCTGGTAGCCATGACGGTGGTGGCCGTGGCGAGATGCCCCCTCAGTGCATGAGGGCACATATCCCGGTGGTGCCTTTAATG GTGACAGTCTCAGGGGCCAGCCAAGCCCCCACCCCCAAGGAAGCCACTGCCTGCCGACCCCCAGGGCCGGTGCCCATCGGGTGACCTGCCCGGCCCAGGG GCTGGAATCCCGCCCCTAGTGGTACCCTCCAGACCAGCGCCACCGCCTCCGACAGTGTCCTCGCTCTACCTCTGACCTCTCCGGAGGTTCCGCTGCCTCC AAGCCGGACTTAGGGCTTCAAGAGGCGGGCGTGCCCTCTGGAGTCCCCTACCATGACTGAAGGCGCCAGAGACTGGCGGTGTCTTAAGACTCCGGGCACC GCCACGCGCTGTCAAGCAACACTCTGCGGACCTGCCGGCGTAGTTGCAGCGGGGGCTTGGGGAGGGGCTGGGGGTTGGACGGGATTGAGGAAGGTCCGCA CAGCCTGTCTCTGCTCAGTTGCAATAAACGTGACATCTTGGGAGCGTTCCCCAGA

ADAM15.F12 (forward primer)
ADAM15.R1 (reverse primer)
Intron retention

Frame 0

LG*AAHCLACSRESLPARPLRARPRTGGREAAK LLGRGPDLAPGEGAPPKPVGPGTGSHSAQLRGSG SAEQRGRSGRCPRSRGTRRAAGRALPGAELGLG PGGRGRAGAGPGGRAALLGSPRRPGRRPLLRAL FRTCCPRPARSAAAMRLALLWALGLLGAGSPLPS WPLPNIGGTEEQQAESEKAPREPLEPQVLQDDL PISLKKVLQTSLPEPLRIKLELDGDSHILELLQ NRELVPGRPTLVWYQPDGTRVVSEGHTLENCCYQ GRVRGYAGSWVSICTCSGLRGLVVLTPERSYTL EQGPGDLQGPPIISRIQDLHLPGHTCALSWRES VHTQKPPEHPLGQRHIRRRRDVVTETKTVELVIV ADHSEAQKYRDFQHLLNRTLEVALLLDTFFRPL NVRVALVGLEAWTQRDLVEISPNPAVTLENFLH WRRAHLLPRLPHDSAQLVTGTSFSGPTVGMAIQN SICSPDFSGGVNMDHSTSILGVASSIAHELGHS LGLDHDLPGNSCPCPGPAPAKTCIMEASTDFLP GLNFSNCSRRALEKALLDGMGSCLFERLPSLPPM AAFCGNMFVEPGEQCDCGFLDDCVDPCCDSLTC QLRPGAQCASDGPCCQNCQLRPSGWQCRPTRGD CDLPEFCPGDSSQCPPDVSLGDGEPCAGGQAVCM HGRCASYAQQCQSLWGPGAQPAAPLCLQTANTR GNAFGSCGRNPSGSYVSCTPRDAICGQLQCQTG RTQPLLGSIRDLLWETIDVNGTELNCSWVHLDLG SDVAQPLLTLPGTACGPGLVCIDHRCQRVDLLG AQECRSKCHGHGVCDSNRHCYCEEGWAPPDCTT QLKATSSLTTGLLLSLLVLLVLVMLGASYWYRAR LHQRLCQLKGPTCQYRAAQSGPSERPGPPQRAL LARGTKQASALSFPAPPSRPLPPDPVSKRLQAE LADRPNPPTRPLPADPVVRSPKVTVGGEKGTASP PT*GCGAGSHDGGGRGEMPPQCMRAHIPVVPLM VTVSGASQAPTPKEATACRPPGPVPIG*PARPR GWNPAPSGTLQTSATASDSVLALPLTSPEVPLPP SRT*GFKRRACPLESPTMTEGARDWRCLKTPGT ATRCQATLCGPAGVVAAGAWGGAGGWTGLRKVR TACLCSVAINVTSWERSP

Frame 1

WGEPRTASPAVGKACPPDLSEPGRAQEAGRPRS Y*GGVRTWRRVKARRPSRSDRAPAPTPHSCGEA VALSSAGVVGGVPAPEAPGAQRGGLCRGRSLAWG RVGGGGPGRGLVAARRCWVLRGDLAAGRSSARC SALAALARPGAPLPCGWRCSGPWGSWARAALCL PGRSQI*VALRSSRQSQRRPRGSPWSPRSFRTIS QLASKRCFRPVCLSP*GSSWSWTVTVISWSCYR IGSWSQAAQPWCGTSPMALGWSVRDTLWRTAAT REECGDMQAPGCPSAPALGSEAWWS*PQREAIPW SRGLGTFRVLPLFRESKISTCQATPVP*AGGNL YTLRSHQSTPWDSATFAGGGMW*QRPRLWSW*L WLITRRPRNTGTSSTC*TAHWKWPSCWTHSSGP* MYEWH*WAWRPGPSVTWWRSAQTQLSPSKTSST GAGHICCLDCPMTVPSW*LVLHSLGLRWAWPFR TPSVLLTSQEV*TWTTPPASWESPPP*PMSWATA WAWTMICLGIAAPVQVQPQPRPASWRPPQTSYQ A*TSATAADGPWRKPSWMEWAAASSNGCLAYPL WLLSAEICLWSRASSVTVASWMTASIPAVIL*PA S*GQVHSVHLTDPVVKIASCARLAGSVVLPEGI VTCLNSAQETAPSVPLMSA*GMASPALAGKLCA CTGVVPPMPSSASHFGDLEPSPLRHFASRQLILG EMLLGAVGATPVAVMCPAPLEMPFVGSSSARQV GPSLCWAPSGIYSGRQ*M*MGLS*TAAGCTWTW AVMWPSPS*LCLAQPVALAWCV*TIDASVWISWG HRNVEANAMDMGSVTATGTATVRRAGHPLTAPL SSKQPAP*PQGCSSASWSYWSW*CLVPATGTVP ACTSDSASSRDPPASTGQPNLVPLNGQDLRRGPC WHEALSRLVLSASRPPLPGRCRLTLCPRDSRLS WLTDPIPLPALCPLTRW*EARR*RWGERRARPL PPPRAVVLVAMTVVAVARCPLSA*GHISRWCL*W *QSQGPAKPPPPRKPLPADPQGRCPSGDLPGPG AGIPPLVVPSRPAPPPPTVSSLYL*PLRRFRCL QAGLRASRGGRALWSPLP*LKAPETGGVLRLRAP PRAVKQHSADLPA*LQRGLGEGLGVGRD*GRSA QPVSAQLQ*T*HLGSVPQ

Frame 2

GVSRALPRLQSGKPARPTSPSQAAHRRQGGREA TREGSGPGAG*RRAAQAGRTGHRLPLRTVAGKR *R*AARA*WAVSPLPRHPARSGAGFAGGGAWLG AGWEGAGRGGAWWPRGAAGFSEATWPPAAPPRAV PHLLPSPGPERRCHAAGAALGPGAPGRGQPSAF LAAPKYRWH*GAAGRVREGPEGALGAPGPSGRS PN*PQKGASDQSA*APEDQVGAGR*QSYPGAATE *GVGPRPPNPGVVPARWHSGGQ*GTHFGELLLP GKSAGICRLLGVHLHLLWAQRLGGPDPREKLYP GAGAWGPSGSSHYFANPRSPPARPHLCPELAGIC THSEATRAPPGTAPHSPEAGCGNRDQDCGVGDC G*SLGGPEIPGLPAPAKPHTGSGPLAGHILPAP ECTSGTSGPGGLDPA*PGGDQPKPSCHPRKLPPL AQGTFAASIAP*QCPAGDWYFILWAYGGHGHSE LHLFS*LLRRCEHGPLHQHPGSRLLHSP*VGPQ PGPGP*FAWE*LPLSRSSPSQDLHHGGLHRLPTR PELQQLQPTGPGESPPGWNGQLPLRTAA*PTPY GCFLRKYVCGAGRAV*LWLPG*LRRSLL*FFDL PAEARCTVCI*RTLLSKLPAAPVWLAVSSYQRGL *LA*ILPRRQLPVSP*CQPRGWRALRWRASCVH ARALCLLCPAVPVTLGTWSPARCATLPPDS*YS GKCFWELWAQPQWQLCVLHP*RCHLWAAPVPDR* DPASAGLHPGSTLGDNRCEWD*AELQLGAPGPG Q*CGPAPPDSAWHSLWPWPGVYRPSMPACGSPG GTGMSKQMPWTWGL*QQQALLL*GGLGTP*LHHS AQSNQLPDHRAAPQPPGLIGPGDAWCQLLVPCP PAPATLPAQGTHLPVQGSPIWSL*TARTSAEGP AGTRH*AG*CSQLPGPPFQAAAA*PCVQETPG*A G*PTQSPYPPSAR*PGGEKPEGNGGGREGHGLS PHLGLWCW*P*RWWPWRDAPSVHEGTYPGGAFN GDSLRGQPSPHPQGSHCLPTPRAGAHRVTCPAQG LESRP*WYPPDQRHRLRQCPRSTSDLSGGSAAS KPDLGLQEAGVPSGVPYHD*RRQRLAVS*DSGH RHALSSNTLRTCRRSCSGGLGRGWGLDGIEEGPH SLSLLSCNKRDILGAFPR

Sequence ADAM15.gAug05

CTGGGGTGAGCCGCGCACTGCCTCGCCTGCAGTCGGGAAAGCCTGCCCGCCCGACCTCTCCGAGCCAGGCCGCGCACAGGAGGCAGGGAGGCCGCGAAGC TACTAGGGAGGGGTCCGGACCTGGCGCCGGGTGAAGGCGCGCCGCCCAAGCCGGTCGGACCGGGCACCGGCTCCCACTCCGCACAGTTGCGGGGAAGCGG TAGCGCTGAGCAGCGCGGGCGTAGTGGGCGGTGTCCCCGCTCCCGAGGCACCCGGCGCGCAGCGGGGCGGGCTTTGCCGGGGGCGGAGCTTGGCTTGGGG CCGGGTGGGAGGGGGCGGGCCGGGGCGGGGCCTGGTGGCCGCGCGGCGCTGCTGGGTTCTCCGAGGCGACCTGGCCGCCGGCCGCTCCTCCGCGCGCTGT TCCGCACTTGCTGCCCTCGCCCGGCCCGGAGCGCCGCTGCCATGCGGCTGGCGCTGCTCTGGGCCCTGGGGCTCCTGGGCGCGGGCAGCCCTCTGCCTTC CTGGCCGCTCCCAAATATAGGTGGCACTGAGGAGCAGCAGGCAGAGTCAGAGAAGGCCCCGAGGGAGCCCTTGGAGCCCCAGGTCCTTCAGGACGATCTC CCAATTAGCCTCAAAAAGGTGCTTCAGACCAGTCTGCCTGAGCCCCTGAGGATCAAGTTGGAGCTGGACGGTGACAGTCATATCCTGGAGCTGCTACAGA ATAGGGAGTTGGTCCCAGGCCGCCCAACCCTGGTGTGGTACCAGCCCGATGGCACTCGGGTGGTCAGTGAGGGACACACTTTGGAGAACTGCTGCTACCA GGGAAGAGTGCGGGGATATGCAGGCTCCTGGGTGTCCATCTGCACCTGCTCTGGGCTCAGAGGCTTGGTGGTCCTGACCCCAGAGAGAAGCTATACCCTG GAGCAGGGGCCTGGGGACCTTCAGGGTCCTCCCATTATTTCGCGAATCCAAGATCTCCACCTGCCAGGCCACACCTGTGCCCTGAGCTGGCGGGAATCTG TACACACTCAGAAGCCACCAGAGCACCCCCTGGGACAGCGCCACATTCGCCGGAGGCGGGATGTGGTAACAGAGACCAAGACTGTGGAGTTGGTGATTGT GGCTGATCACTCGGAGGCCCAGAAATACCGGGACTTCCAGCACCTGCTAAACCGCACACTGGAAGTGGCCCTCTTGCTGGACACATTCTTCCGGCCCCTG AATGTACGAGTGGCACTAGTGGGCCTGGAGGCCTGGACCCAGCGTGACCTGGTGGAGATCAGCCCAAACCCAGCTGTCACCCTCGAAAACTTCCTCCACT GGCGCAGGGCACATTTGCTGCCTCGATTGCCCCATGACAGTGCCCAGCTGGTGACTGGTACTTCATTCTCTGGGCCTACGGTGGGCATGGCCATTCAGAA CTCCATCTGTTCTCCTGACTTCTCAGGAGGTGTGAACATGGACCACTCCACCAGCATCCTGGGAGTCGCCTCCTCCATAGCCCATGAGTTGGGCCACAGC CTGGGCCTGGACCATGATTTGCCTGGGAATAGCTGCCCCTGTCCAGGTCCAGCCCCAGCCAAGACCTGCATCATGGAGGCCTCCACAGACTTCCTACCAG GCCTGAACTTCAGCAACTGCAGCCGACGGGCCCTGGAGAAAGCCCTCCTGGATGGAATGGGCAGCTGCCTCTTCGAACGGCTGCCTAGCCTACCCCCTAT GGCTGCTTTCTGCGGAAATATGTTTGTGGAGCCGGGCGAGCAGTGTGACTGTGGCTTCCTGGATGACTGCGTCGATCCCTGCTGTGATTCTTTGACCTGC CAGCTGAGGCCAGGTGCACAGTGTGCATCTGACGGACCCTGTTGTCAAAATTGCCAGCTGCGCCCGTCTGGCTGGCAGTGTCGTCCTACCAGAGGGGATT GTGACTTGCCTGAATTCTGCCCAGGAGACAGCTCCCAGTGTCCCCCTGATGTCAGCCTAGGGGATGGCGAGCCCTGCGCTGGCGGGCAAGCTGTGTGCAT GCACGGGCGTTGTGCCTCCTATGCCCAGCAGTGCCAGTCACTTTGGGGACCTGGAGCCCAGCCCGCTGCGCCACTTTGCCTCCAGACAGCTAATACTCGG GGAAATGCTTTTGGGAGCTGTGGGCGCAACCCCAGTGGCAGTTATGTGTCCTGCACCCCTAGAGATGCCATTTGTGGGCAGCTCCAGTGCCAGACAGGTA GGACCCAGCCTCTGCTGGGCTCCATCCGGGATCTACTCTGGGAGACAATAGATGTGAATGGGACTGAGCTGAACTGCAGCTGGGTGCACCTGGACCTGGG CAGTGATGTGGCCCAGCCCCTCCTGACTCTGCCTGGCACAGCCTGTGGCCCTGGCCTGGTGTGTATAGACCATCGATGCCAGCGTGTGGATCTCCTGGGG GCACAGGAATGTCGAAGCAAATGCCATGGACATGGGGTCTGTGACAGCAACAGGCACTGCTACTGTGAGGAGGGCTGGGCACCCCCTGACTGCACCACTC AGCTCAAAGCAACCAGCTCCCTGACCACAGGGCTGCTCCTCAGCCTCCTGGTCTTATTGGTCCTGGTGATGCTTGGTGCCAGCTACTGGTACCGTGCCCG CCTGCACCAGCGACTCTGCCAGCTCAAGGGACCCACCTGCCAGTACAGGGCAGCCCAATCTGGTCCCTCTGAACGGCCAGGACCTCCGCAGAGGGCCCTG CTGGCACGAGGCACTAAGGCTAGTGCTCTCAGCTTCCCGGCCCCCCCTTCCAGGCCGCTGCCGCCTGACCCTGTGTCCAAGAGACTCCAGGCTGAGCTGG CTGACCGACCCAATCCCCCTACCCGCCCTCTGCCCGCTGACCCGGTGGTGAGAAGCCCGAAGGTAACGGTGGGGGGAGAGAAGGGCACGGCCTCTCCCCC CACCTAGGGCTGTGGTGCTGGTAGCCATGACGGTGGTGGCCGTGGCGAGATGCCCCCTCAGTGCATGAGGGCACATATCCCGGTGGTGCCTTTAATGGTG ACAGTCTCAGGGGCCAGCCAAGCCCCCACCCCCAAGGAAGCCACTGCCTGCCGACCCCCAGGGCCGGTGCCCATCGGGTGACCTGCCCGGCCCAGGGGCT GGAATCCCGCCCCTAGTGGTACCCTCCAGACCAGCGCCACCGCCTCCGACAGTGTCCTCGCTCTACCTCTGACCTCTCCGGAGGTTCCGCTGCCTCCAAG CCGGACTTAGGGCTTCAAGAGGCGGGCGTGCCCTCTGGAGTCCCCTACCATGACTGAAGGCGCCAGAGACTGGCGGTGTCTTAAGACTCCGGGCACCGCC ACGCGCTGTCAAGCAACACTCTGCGGACCTGCCGGCGTAGTTGCAGCGGGGGCTTGGGGAGGGGCTGGGGGTTGGACGGGATTGAGGAAGGTCCGCACAG CCTGTCTCTGCTCAGTTGCAATAAACGTGACATCTTGGGAGCGTTCCCCAGA

ADAM15.F12 (forward primer)
ADAM15.R1 (reverse primer)
Intron retention

Frame 0

LG*AAHCLACSRESLPARPLRARPRTGGREAAK LLGRGPDLAPGEGAPPKPVGPGTGSHSAQLRGSG SAEQRGRSGRCPRSRGTRRAAGRALPGAELGLG PGGRGRAGAGPGGRAALLGSPRRPGRRPLLRAL FRTCCPRPARSAAAMRLALLWALGLLGAGSPLPS WPLPNIGGTEEQQAESEKAPREPLEPQVLQDDL PISLKKVLQTSLPEPLRIKLELDGDSHILELLQ NRELVPGRPTLVWYQPDGTRVVSEGHTLENCCYQ GRVRGYAGSWVSICTCSGLRGLVVLTPERSYTL EQGPGDLQGPPIISRIQDLHLPGHTCALSWRES VHTQKPPEHPLGQRHIRRRRDVVTETKTVELVIV ADHSEAQKYRDFQHLLNRTLEVALLLDTFFRPL NVRVALVGLEAWTQRDLVEISPNPAVTLENFLH WRRAHLLPRLPHDSAQLVTGTSFSGPTVGMAIQN SICSPDFSGGVNMDHSTSILGVASSIAHELGHS LGLDHDLPGNSCPCPGPAPAKTCIMEASTDFLP GLNFSNCSRRALEKALLDGMGSCLFERLPSLPPM AAFCGNMFVEPGEQCDCGFLDDCVDPCCDSLTC QLRPGAQCASDGPCCQNCQLRPSGWQCRPTRGD CDLPEFCPGDSSQCPPDVSLGDGEPCAGGQAVCM HGRCASYAQQCQSLWGPGAQPAAPLCLQTANTR GNAFGSCGRNPSGSYVSCTPRDAICGQLQCQTG RTQPLLGSIRDLLWETIDVNGTELNCSWVHLDLG SDVAQPLLTLPGTACGPGLVCIDHRCQRVDLLG AQECRSKCHGHGVCDSNRHCYCEEGWAPPDCTT QLKATSSLTTGLLLSLLVLLVLVMLGASYWYRAR LHQRLCQLKGPTCQYRAAQSGPSERPGPPQRAL LARGTKASALSFPAPPSRPLPPDPVSKRLQAEL ADRPNPPTRPLPADPVVRSPKVTVGGEKGTASPP T*GCGAGSHDGGGRGEMPPQCMRAHIPVVPLMV TVSGASQAPTPKEATACRPPGPVPIG*PARPRG WNPAPSGTLQTSATASDSVLALPLTSPEVPLPPS RT*GFKRRACPLESPTMTEGARDWRCLKTPGTA TRCQATLCGPAGVVAAGAWGGAGGWTGLRKVRT ACLCSVAINVTSWERSP

Frame 1

WGEPRTASPAVGKACPPDLSEPGRAQEAGRPRS Y*GGVRTWRRVKARRPSRSDRAPAPTPHSCGEA VALSSAGVVGGVPAPEAPGAQRGGLCRGRSLAWG RVGGGGPGRGLVAARRCWVLRGDLAAGRSSARC SALAALARPGAPLPCGWRCSGPWGSWARAALCL PGRSQI*VALRSSRQSQRRPRGSPWSPRSFRTIS QLASKRCFRPVCLSP*GSSWSWTVTVISWSCYR IGSWSQAAQPWCGTSPMALGWSVRDTLWRTAAT REECGDMQAPGCPSAPALGSEAWWS*PQREAIPW SRGLGTFRVLPLFRESKISTCQATPVP*AGGNL YTLRSHQSTPWDSATFAGGGMW*QRPRLWSW*L WLITRRPRNTGTSSTC*TAHWKWPSCWTHSSGP* MYEWH*WAWRPGPSVTWWRSAQTQLSPSKTSST GAGHICCLDCPMTVPSW*LVLHSLGLRWAWPFR TPSVLLTSQEV*TWTTPPASWESPPP*PMSWATA WAWTMICLGIAAPVQVQPQPRPASWRPPQTSYQ A*TSATAADGPWRKPSWMEWAAASSNGCLAYPL WLLSAEICLWSRASSVTVASWMTASIPAVIL*PA S*GQVHSVHLTDPVVKIASCARLAGSVVLPEGI VTCLNSAQETAPSVPLMSA*GMASPALAGKLCA CTGVVPPMPSSASHFGDLEPSPLRHFASRQLILG EMLLGAVGATPVAVMCPAPLEMPFVGSSSARQV GPSLCWAPSGIYSGRQ*M*MGLS*TAAGCTWTW AVMWPSPS*LCLAQPVALAWCV*TIDASVWISWG HRNVEANAMDMGSVTATGTATVRRAGHPLTAPL SSKQPAP*PQGCSSASWSYWSW*CLVPATGTVP ACTSDSASSRDPPASTGQPNLVPLNGQDLRRGPC WHEALRLVLSASRPPLPGRCRLTLCPRDSRLSW LTDPIPLPALCPLTRW*EARR*RWGERRARPLP PPRAVVLVAMTVVAVARCPLSA*GHISRWCL*W* QSQGPAKPPPPRKPLPADPQGRCPSGDLPGPGA GIPPLVVPSRPAPPPPTVSSLYL*PLRRFRCLQ AGLRASRGGRALWSPLP*LKAPETGGVLRLRAPP RAVKQHSADLPA*LQRGLGEGLGVGRD*GRSAQ PVSAQLQ*T*HLGSVPQ

Frame 2

GVSRALPRLQSGKPARPTSPSQAAHRRQGGREA TREGSGPGAG*RRAAQAGRTGHRLPLRTVAGKR *R*AARA*WAVSPLPRHPARSGAGFAGGGAWLG AGWEGAGRGGAWWPRGAAGFSEATWPPAAPPRAV PHLLPSPGPERRCHAAGAALGPGAPGRGQPSAF LAAPKYRWH*GAAGRVREGPEGALGAPGPSGRS PN*PQKGASDQSA*APEDQVGAGR*QSYPGAATE *GVGPRPPNPGVVPARWHSGGQ*GTHFGELLLP GKSAGICRLLGVHLHLLWAQRLGGPDPREKLYP GAGAWGPSGSSHYFANPRSPPARPHLCPELAGIC THSEATRAPPGTAPHSPEAGCGNRDQDCGVGDC G*SLGGPEIPGLPAPAKPHTGSGPLAGHILPAP ECTSGTSGPGGLDPA*PGGDQPKPSCHPRKLPPL AQGTFAASIAP*QCPAGDWYFILWAYGGHGHSE LHLFS*LLRRCEHGPLHQHPGSRLLHSP*VGPQ PGPGP*FAWE*LPLSRSSPSQDLHHGGLHRLPTR PELQQLQPTGPGESPPGWNGQLPLRTAA*PTPY GCFLRKYVCGAGRAV*LWLPG*LRRSLL*FFDL PAEARCTVCI*RTLLSKLPAAPVWLAVSSYQRGL *LA*ILPRRQLPVSP*CQPRGWRALRWRASCVH ARALCLLCPAVPVTLGTWSPARCATLPPDS*YS GKCFWELWAQPQWQLCVLHP*RCHLWAAPVPDR* DPASAGLHPGSTLGDNRCEWD*AELQLGAPGPG Q*CGPAPPDSAWHSLWPWPGVYRPSMPACGSPG GTGMSKQMPWTWGL*QQQALLL*GGLGTP*LHHS AQSNQLPDHRAAPQPPGLIGPGDAWCQLLVPCP PAPATLPAQGTHLPVQGSPIWSL*TARTSAEGP AGTRH*G*CSQLPGPPFQAAAA*PCVQETPG*AG *PTQSPYPPSAR*PGGEKPEGNGGGREGHGLSP HLGLWCW*P*RWWPWRDAPSVHEGTYPGGAFNG DSLRGQPSPHPQGSHCLPTPRAGAHRVTCPAQGL ESRP*WYPPDQRHRLRQCPRSTSDLSGGSAASK PDLGLQEAGVPSGVPYHD*RRQRLAVS*DSGHR HALSSNTLRTCRRSCSGGLGRGWGLDGIEEGPHS LSLLSCNKRDILGAFPR

Sequence ADAM15.iAug05

CTGGGGTGAGCCGCGCACTGCCTCGCCTGCAGTCGGGAAAGCCTGCCCGCCCGACCTCTCCGAGCCAGGCCGCGCACAGGAGGCAGGGAGGCCGCGAAGC TACTAGGGAGGGGTCCGGACCTGGCGCCGGGTGAAGGCGCGCCGCCCAAGCCGGTCGGACCGGGCACCGGCTCCCACTCCGCACAGTTGCGGGGAAGCGG TAGCGCTGAGCAGCGCGGGCGTAGTGGGCGGTGTCCCCGCTCCCGAGGCACCCGGCGCGCAGCGGGGCGGGCTTTGCCGGGGGCGGAGCTTGGCTTGGGG CCGGGTGGGAGGGGGCGGGCCGGGGCGGGGCCTGGTGGCCGCGCGGCGCTGCTGGGTTCTCCGAGGCGACCTGGCCGCCGGCCGCTCCTCCGCGCGCTGT TCCGCACTTGCTGCCCTCGCCCGGCCCGGAGCGCCGCTGCCATGCGGCTGGCGCTGCTCTGGGCCCTGGGGCTCCTGGGCGCGGGCAGCCCTCTGCCTTC CTGGCCGCTCCCAAATATAGGTGGCACTGAGGAGCAGCAGGCAGAGTCAGAGAAGGCCCCGAGGGAGCCCTTGGAGCCCCAGGTCCTTCAGGACGATCTC CCAATTAGCCTCAAAAAGGTGCTTCAGACCAGTCTGCCTGAGCCCCTGAGGATCAAGTTGGAGCTGGACGGTGACAGTCATATCCTGGAGCTGCTACAGA ATAGGGAGTTGGTCCCAGGCCGCCCAACCCTGGTGTGGTACCAGCCCGATGGCACTCGGGTGGTCAGTGAGGGACACACTTTGGAGAACTGCTGCTACCA GGGAAGAGTGCGGGGATATGCAGGCTCCTGGGTGTCCATCTGCACCTGCTCTGGGCTCAGAGGCTTGGTGGTCCTGACCCCAGAGAGAAGCTATACCCTG GAGCAGGGGCCTGGGGACCTTCAGGGTCCTCCCATTATTTCGCGAATCCAAGATCTCCACCTGCCAGGCCACACCTGTGCCCTGAGCTGGCGGGAATCTG TACACACTCAGAAGCCACCAGAGCACCCCCTGGGACAGCGCCACATTCGCCGGAGGCGGGATGTGGTAACAGAGACCAAGACTGTGGAGTTGGTGATTGT GGCTGATCACTCGGAGGCCCAGAAATACCGGGACTTCCAGCACCTGCTAAACCGCACACTGGAAGTGGCCCTCTTGCTGGACACATTCTTCCGGCCCCTG AATGTACGAGTGGCACTAGTGGGCCTGGAGGCCTGGACCCAGCGTGACCTGGTGGAGATCAGCCCAAACCCAGCTGTCACCCTCGAAAACTTCCTCCACT GGCGCAGGGCACATTTGCTGCCTCGATTGCCCCATGACAGTGCCCAGCTGGTGACTGGTACTTCATTCTCTGGGCCTACGGTGGGCATGGCCATTCAGAA CTCCATCTGTTCTCCTGACTTCTCAGGAGGTGTGAACATGGACCACTCCACCAGCATCCTGGGAGTCGCCTCCTCCATAGCCCATGAGTTGGGCCACAGC CTGGGCCTGGACCATGATTTGCCTGGGAATAGCTGCCCCTGTCCAGGTCCAGCCCCAGCCAAGACCTGCATCATGGAGGCCTCCACAGACTTCCTACCAG GCCTGAACTTCAGCAACTGCAGCCGACGGGCCCTGGAGAAAGCCCTCCTGGATGGAATGGGCAGCTGCCTCTTCGAACGGCTGCCTAGCCTACCCCCTAT GGCTGCTTTCTGCGGAAATATGTTTGTGGAGCCGGGCGAGCAGTGTGACTGTGGCTTCCTGGATGACTGCGTCGATCCCTGCTGTGATTCTTTGACCTGC CAGCTGAGGCCAGGTGCACAGTGTGCATCTGACGGACCCTGTTGTCAAAATTGCCAGCTGCGCCCGTCTGGCTGGCAGTGTCGTCCTACCAGAGGGGATT GTGACTTGCCTGAATTCTGCCCAGGAGACAGCTCCCAGTGTCCCCCTGATGTCAGCCTAGGGGATGGCGAGCCCTGCGCTGGCGGGCAAGCTGTGTGCAT GCACGGGCGTTGTGCCTCCTATGCCCAGCAGTGCCAGTCACTTTGGGGACCTGGAGCCCAGCCCGCTGCGCCACTTTGCCTCCAGACAGCTAATACTCGG GGAAATGCTTTTGGGAGCTGTGGGCGCAACCCCAGTGGCAGTTATGTGTCCTGCACCCCTAGAGATGCCATTTGTGGGCAGCTCCAGTGCCAGACAGGTA GGACCCAGCCTCTGCTGGGCTCCATCCGGGATCTACTCTGGGAGACAATAGATGTGAATGGGACTGAGCTGAACTGCAGCTGGGTGCACCTGGACCTGGG CAGTGATGTGGCCCAGCCCCTCCTGACTCTGCCTGGCACAGCCTGTGGCCCTGGCCTGGTGTGTATAGACCATCGATGCCAGCGTGTGGATCTCCTGGGG GCACAGGAATGTCGAAGCAAATGCCATGGACATGGGGTCTGTGACAGCAACAGGCACTGCTACTGTGAGGAGGGCTGGGCACCCCCTGACTGCACCACTC AGCTCAAAGCAACCAGCTCCCTGACCACAGGGCTGCTCCTCAGCCTCCTGGTCTTATTGGTCCTGGTGATGCTTGGTGCCAGCTACTGGTACCGTGCCCG CCTGCACCAGCGACTCTGCCAGCTCAAGGGACCCACCTGCCAGTACAGGGCAGCCCAATCTGGTCCCTCTGAACGGCCAGGACCTCCGCAGAGGGCCCTG CTGGCACGAGGCACTAAGGCTGAGCTGGCTGACCGACCCAATCCCCCTACCCGCCCTCTGCCCGCTGACCCGGTGGTGAGAAGCCCGAAGGTAACGGTGG GGGGAGAGAAGGGCACGGCCTCTCCCCCCACCTAGGGCTGTGGTGCTGGTAGCCATGACGGTGGTGGCCGTGGCGAGATGCCCCCTCAGTGCATGAGGGC ACATATCCCGGTGGTGCCTTTAATGGTGACAGTCTCAGGGGCCAGCCAAGCCCCCACCCCCAAGGAAGCCACTGCCTGCCGACCCCCAGGGCCGGTGCCC ATCGGGTGACCTGCCCGGCCCAGGGGCTGGAATCCCGCCCCTAGTGGTACCCTCCAGACCAGCGCCACCGCCTCCGACAGTGTCCTCGCTCTACCTCTGA CCTCTCCGGAGGTTCCGCTGCCTCCAAGCCGGACTTAGGGCTTCAAGAGGCGGGCGTGCCCTCTGGAGTCCCCTACCATGACTGAAGGCGCCAGAGACTG GCGGTGTCTTAAGACTCCGGGCACCGCCACGCGCTGTCAAGCAACACTCTGCGGACCTGCCGGCGTAGTTGCAGCGGGGGCTTGGGGAGGGGCTGGGGGT TGGACGGGATTGAGGAAGGTCCGCACAGCCTGTCTCTGCTCAGTTGCAATAAACGTGACATCTTGGGAGCGTTCCCCAGA

ADAM15.F12 (forward primer)
ADAM15.R1 (reverse primer)
Intron retention

Frame 0

LG*AAHCLACSRESLPARPLRARPRTGGREAAK LLGRGPDLAPGEGAPPKPVGPGTGSHSAQLRGSG SAEQRGRSGRCPRSRGTRRAAGRALPGAELGLG PGGRGRAGAGPGGRAALLGSPRRPGRRPLLRAL FRTCCPRPARSAAAMRLALLWALGLLGAGSPLPS WPLPNIGGTEEQQAESEKAPREPLEPQVLQDDL PISLKKVLQTSLPEPLRIKLELDGDSHILELLQ NRELVPGRPTLVWYQPDGTRVVSEGHTLENCCYQ GRVRGYAGSWVSICTCSGLRGLVVLTPERSYTL EQGPGDLQGPPIISRIQDLHLPGHTCALSWRES VHTQKPPEHPLGQRHIRRRRDVVTETKTVELVIV ADHSEAQKYRDFQHLLNRTLEVALLLDTFFRPL NVRVALVGLEAWTQRDLVEISPNPAVTLENFLH WRRAHLLPRLPHDSAQLVTGTSFSGPTVGMAIQN SICSPDFSGGVNMDHSTSILGVASSIAHELGHS LGLDHDLPGNSCPCPGPAPAKTCIMEASTDFLP GLNFSNCSRRALEKALLDGMGSCLFERLPSLPPM AAFCGNMFVEPGEQCDCGFLDDCVDPCCDSLTC QLRPGAQCASDGPCCQNCQLRPSGWQCRPTRGD CDLPEFCPGDSSQCPPDVSLGDGEPCAGGQAVCM HGRCASYAQQCQSLWGPGAQPAAPLCLQTANTR GNAFGSCGRNPSGSYVSCTPRDAICGQLQCQTG RTQPLLGSIRDLLWETIDVNGTELNCSWVHLDLG SDVAQPLLTLPGTACGPGLVCIDHRCQRVDLLG AQECRSKCHGHGVCDSNRHCYCEEGWAPPDCTT QLKATSSLTTGLLLSLLVLLVLVMLGASYWYRAR LHQRLCQLKGPTCQYRAAQSGPSERPGPPQRAL LARGTKAELADRPNPPTRPLPADPVVRSPKVTV GGEKGTASPPT*GCGAGSHDGGGRGEMPPQCMRA HIPVVPLMVTVSGASQAPTPKEATACRPPGPVP IG*PARPRGWNPAPSGTLQTSATASDSVLALPL TSPEVPLPPSRT*GFKRRACPLESPTMTEGARDW RCLKTPGTATRCQATLCGPAGVVAAGAWGGAGG WTGLRKVRTACLCSVAINVTSWERSP

Frame 1

WGEPRTASPAVGKACPPDLSEPGRAQEAGRPRS Y*GGVRTWRRVKARRPSRSDRAPAPTPHSCGEA VALSSAGVVGGVPAPEAPGAQRGGLCRGRSLAWG RVGGGGPGRGLVAARRCWVLRGDLAAGRSSARC SALAALARPGAPLPCGWRCSGPWGSWARAALCL PGRSQI*VALRSSRQSQRRPRGSPWSPRSFRTIS QLASKRCFRPVCLSP*GSSWSWTVTVISWSCYR IGSWSQAAQPWCGTSPMALGWSVRDTLWRTAAT REECGDMQAPGCPSAPALGSEAWWS*PQREAIPW SRGLGTFRVLPLFRESKISTCQATPVP*AGGNL YTLRSHQSTPWDSATFAGGGMW*QRPRLWSW*L WLITRRPRNTGTSSTC*TAHWKWPSCWTHSSGP* MYEWH*WAWRPGPSVTWWRSAQTQLSPSKTSST GAGHICCLDCPMTVPSW*LVLHSLGLRWAWPFR TPSVLLTSQEV*TWTTPPASWESPPP*PMSWATA WAWTMICLGIAAPVQVQPQPRPASWRPPQTSYQ A*TSATAADGPWRKPSWMEWAAASSNGCLAYPL WLLSAEICLWSRASSVTVASWMTASIPAVIL*PA S*GQVHSVHLTDPVVKIASCARLAGSVVLPEGI VTCLNSAQETAPSVPLMSA*GMASPALAGKLCA CTGVVPPMPSSASHFGDLEPSPLRHFASRQLILG EMLLGAVGATPVAVMCPAPLEMPFVGSSSARQV GPSLCWAPSGIYSGRQ*M*MGLS*TAAGCTWTW AVMWPSPS*LCLAQPVALAWCV*TIDASVWISWG HRNVEANAMDMGSVTATGTATVRRAGHPLTAPL SSKQPAP*PQGCSSASWSYWSW*CLVPATGTVP ACTSDSASSRDPPASTGQPNLVPLNGQDLRRGPC WHEALRLSWLTDPIPLPALCPLTRW*EARR*RW GERRARPLPPPRAVVLVAMTVVAVARCPLSA*G HISRWCL*W*QSQGPAKPPPPRKPLPADPQGRCP SGDLPGPGAGIPPLVVPSRPAPPPPTVSSLYL* PLRRFRCLQAGLRASRGGRALWSPLP*LKAPET GGVLRLRAPPRAVKQHSADLPA*LQRGLGEGLGV GRD*GRSAQPVSAQLQ*T*HLGSVPQ

Frame 2

GVSRALPRLQSGKPARPTSPSQAAHRRQGGREA TREGSGPGAG*RRAAQAGRTGHRLPLRTVAGKR *R*AARA*WAVSPLPRHPARSGAGFAGGGAWLG AGWEGAGRGGAWWPRGAAGFSEATWPPAAPPRAV PHLLPSPGPERRCHAAGAALGPGAPGRGQPSAF LAAPKYRWH*GAAGRVREGPEGALGAPGPSGRS PN*PQKGASDQSA*APEDQVGAGR*QSYPGAATE *GVGPRPPNPGVVPARWHSGGQ*GTHFGELLLP GKSAGICRLLGVHLHLLWAQRLGGPDPREKLYP GAGAWGPSGSSHYFANPRSPPARPHLCPELAGIC THSEATRAPPGTAPHSPEAGCGNRDQDCGVGDC G*SLGGPEIPGLPAPAKPHTGSGPLAGHILPAP ECTSGTSGPGGLDPA*PGGDQPKPSCHPRKLPPL AQGTFAASIAP*QCPAGDWYFILWAYGGHGHSE LHLFS*LLRRCEHGPLHQHPGSRLLHSP*VGPQ PGPGP*FAWE*LPLSRSSPSQDLHHGGLHRLPTR PELQQLQPTGPGESPPGWNGQLPLRTAA*PTPY GCFLRKYVCGAGRAV*LWLPG*LRRSLL*FFDL PAEARCTVCI*RTLLSKLPAAPVWLAVSSYQRGL *LA*ILPRRQLPVSP*CQPRGWRALRWRASCVH ARALCLLCPAVPVTLGTWSPARCATLPPDS*YS GKCFWELWAQPQWQLCVLHP*RCHLWAAPVPDR* DPASAGLHPGSTLGDNRCEWD*AELQLGAPGPG Q*CGPAPPDSAWHSLWPWPGVYRPSMPACGSPG GTGMSKQMPWTWGL*QQQALLL*GGLGTP*LHHS AQSNQLPDHRAAPQPPGLIGPGDAWCQLLVPCP PAPATLPAQGTHLPVQGSPIWSL*TARTSAEGP AGTRH*G*AG*PTQSPYPPSAR*PGGEKPEGNGG GREGHGLSPHLGLWCW*P*RWWPWRDAPSVHEG TYPGGAFNGDSLRGQPSPHPQGSHCLPTPRAGA HRVTCPAQGLESRP*WYPPDQRHRLRQCPRSTSD LSGGSAASKPDLGLQEAGVPSGVPYHD*RRQRL AVS*DSGHRHALSSNTLRTCRRSCSGGLGRGWG LDGIEEGPHSLSLLSCNKRDILGAFPR

Sequence ADAM15.uAug05

CATGCTTTATCTTGCCCCCTCGGCCCTCTCTTTCTGACCCCCCTTTGCTGCTGGTTCCCTGAGTCTGTGCCCCTTGAACCCTTCACTCTCCCTGATCACT TGACTTCACTCCCTGCCCCCTGCGCCTTCATGGATCTAGAGTTCTTAGCCCCGTCCTCCCTTAAACCTGACTTCCGCCCACAGCAACCAGCTCCCTGACC ACAGGGCTGCTCCTCAGCCTCCTGGTCTTATTGGTCCTGGTGATGCTTGGTGCCAGCTACTGGTACCGTGCCCGCCTGCACCAGCGACTCTGCCAGCTCA AGGGACCCACCTGCCAGTACAGGGCAGCCCAATCTGGTCCCTCTGAACGGCCAGGACCTCCGCAGAGGGCCCTGCTGGCACGAGGCACTAAGCAGGCTAG TGCTCTCAGCTTCCCGGCCCCCCCTTCCAGGCCGCTGCCGCCTGACCCTGTGTCCAAGAGACTCCAGGTAAATCTGGGCCAGGGCCCGCCCTGAGCCAAG GCAGGTGGGAGGCTTGGTACCCAACTGCAGTTCTCATCCCTGCTCCCTGCCAGTGGTGCTCTTCATTAGGTGATCGGGGTGCCCCTCAGCCTTCAGACAC TCTGAGCCCCAGAAGCAGCAAAGGGTGAGCCTCCTGCCTTGGTTTCTACCACCATCTGGTGGTCAATGGCGGGACTGCAGTCGGCCAGGGACTGCGGTTG ACCTACACCTTCCTCCCCTGAGACATCAGCTGGCATGCCTCCAAGCCCTCTGCGCATGCCCTCATTCTCTCCTGCCCCCTGGCTGACTTTGCATGTTGAC TTGAACCCCTCTGGGGAATCTCTGTGCATGTCCACAGCCCCTGGTTATGCTCTCACAGCCACTGCCCCTCTCTCTGTTCAGGCTGAGCTGGCTGACCGAC CCAATCCCCCTACCCGCCCTCTGCCCGCTGACCCGGTGGTGAGAAGCCCGAAGGTAACGGTGGGGGGAGAGAAGGGCACGGCCTCTCCCCCCACCTAGGG CTGTGGTGCTGGTAGCCATGACGGTGGTGGCCGTGGCGAGATGCCCCCTCAGTGCATGAGGGCACATATCCCGGTGGTGCCTTTAATGGTGACAGGTTTG TTTGCAGACAAGGGTGACCGCTGGTGCTGCCCCCAAGCTGGTGTTCCCCAGCACCAGTGCGTTGGGGGTGGGCAACATGACACCCCCCCACCTAAGCCGG CCTCCTGCCTTCTCTCCCTCAGTCTCAGGGGCCAGCCAAGCCCCCACCCCCAAGGAAGCCACTGCCTGCCGACCCCCAGGGCCGGTGCCCATCGGGTGAC CTGCCCGGCCCAGGGGCTGGAATCCCGCCCCTAGTGGTACCCTCCAGACCAGCGCCACCGCCTCCGACAGTGTCCTCGCTCTACCTCTGACCTCTCCGGA GGTTCCGCTGCCTCCAAGCCGGACTTAGGGCTTCAAGAGGCGGGCGTGCCCTCTGGAGTCCCCTACCATGACTGAAGGCGCCAGAGACTGGCGGTGTCTT AAGACTCCGGGCACCGCCACGCGCTGTCAAGCAACACTCTGCGGACCTGCCGGCGTAGTTGCAGCGGGGGCTTGGGGAGGGGCTGGGGGTTGGACGGGAT TGAGGAAGGTCCGCACAGCCTGTCTCTGCTCAGTTGCAATAAACGTGACATCTTGGGAGCGTTCCCCAGA

ADAM15.F12 (forward primer)
ADAM15.R1 (reverse primer)
Intron retention

Frame 0

HALSCPLGPLFLTPLCCWFPESVPLEPFTLPDH LTSLPAPCAFMDLEFLAPSSLKPDFRPQQPAP*P QGCSSASWSYWSW*CLVPATGTVPACTSDSASS RDPPASTGQPNLVPLNGQDLRRGPCWHEALSRL VLSASRPPLPGRCRLTLCPRDSR*IWARARPEPR QVGGLVPNCSSHPCSLPVVLFIR*SGCPSAFRH SEPQKQQRVSLLPWFLPPSGGQWRDCSRPGTAV DLHLPPLRHQLACLQALCACPHSLLPPG*LCMLT *TPLGNLCACPQPLVMLSQPLPLSLFRLSWLTD PIPLPALCPLTRW*EARR*RWGERRARPLPPPR AVVLVAMTVVAVARCPLSA*GHISRWCL*W*QVC LQTRVTAGAAPKLVFPSTSALGVGNMTPPHLSR PPAFSPSVSGASQAPTPKEATACRPPGPVPIG* PARPRGWNPAPSGTLQTSATASDSVLALPLTSPE VPLPPSRT*GFKRRACPLESPTMTEGARDWRCL KTPGTATRCQATLCGPAGVVAAGAWGGAGGWTG LRKVRTACLCSVAINVTSWERSP

Frame 1

MLYLAPSALSF*PPFAAGSLSLCPLNPSLSLIT *LHSLPPAPSWI*SS*PRPPLNLTSAHSNQLPD HRAAPQPPGLIGPGDAWCQLLVPCPPAPATLPAQ GTHLPVQGSPIWSL*TARTSAEGPAGTRH*AG* CSQLPGPPFQAAAA*PCVQETPGKSGPGPALSQ GRWEAWYPTAVLIPAPCQWCSSLGDRGAPQPSDT LSPRSSKG*ASCLGFYHHLVVNGGTAVGQGLRL TYTFLP*DISWHASKPSAHALILSCPLADFAC* LEPLWGISVHVHSPWLCSHSHCPSLCSG*AG*PT QSPYPPSAR*PGGEKPEGNGGGREGHGLSPHLG LWCW*P*RWWPWRDAPSVHEGTYPGGAFNGDRF VCRQG*PLVLPPSWCSPAPVRWGWAT*HPPT*AG LLPSLPQSQGPAKPPPPRKPLPADPQGRCPSGD LPGPGAGIPPLVVPSRPAPPPPTVSSLYL*PLR RFRCLQAGLRASRGGRALWSPLP*LKAPETGGVL RLRAPPRAVKQHSADLPA*LQRGLGEGLGVGRD *GRSAQPVSAQLQ*T*HLGSVPQ

Frame 2

CFILPPRPSLSDPPLLLVP*VCAP*TLHSP*SL DFTPCPLRLHGSRVLSPVLP*T*LPPTATSSLT TGLLLSLLVLLVLVMLGASYWYRARLHQRLCQL KGPTCQYRAAQSGPSERPGPPQRALLARGTKQAS ALSFPAPPSRPLPPDPVSKRLQVNLGQGPP*AK AGGRLGTQLQFSSLLPASGALH*VIGVPLSLQT L*APEAAKGEPPALVSTTIWWSMAGLQSARDCG* PTPSSPETSAGMPPSPLRMPSFSPAPWLTLHVD LNPSGESLCMSTAPGYALTATAPLSVQAELADR PNPPTRPLPADPVVRSPKVTVGGEKGTASPPT*G CGAGSHDGGGRGEMPPQCMRAHIPVVPLMVTGL FADKGDRWCCPQAGVPQHQCVGGGQHDTPPPKP ASCLLSLSLRGQPSPHPQGSHCLPTPRAGAHRVT CPAQGLESRP*WYPPDQRHRLRQCPRSTSDLSG GSAASKPDLGLQEAGVPSGVPYHD*RRQRLAVS *DSGHRHALSSNTLRTCRRSCSGGLGRGWGLDGI EEGPHSLSLLSCNKRDILGAFPR