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Sequences

Sequence APP.fAug05

CTGCAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACCCATCCCCACTTTGTGA
TTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCAAATTCTTACACCAGGAGAGGATGGATGTTTGCGAAAC
TCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAACTTGCATGACTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTC
CGAGGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGCTGATGCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAG
CAGACACAGACTATGCAGATGGGAGTGAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAGTGGCTGAGGTGGAAGAAGAAGAAGCCGATGATGACGA
GGACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGAGGAACCCTACGAAGAAGCCACAGAGAGAACCACCAGCATTGCCACCACCACCACCACCACC
ACAGAGTCTGTGGAAGAGGTGGTTCGAGAGGTGTGCTCTGAACAAGCCGAGACGGGGCCGTGCCGAGCAATGATCTCCCGCTGGTACTTTGATGTGACTG
AAGGGAAGTGTGCCCCATTCTTTTACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAGAAGAGTACTGCATGGCCGTGTGTGGCAGCGCCATTCC
TACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCGAGACACCTGGGGATGAGAATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTT
GAGGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAAGAGGCAGAACGTCAAGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTA
TCCAGCATTTCCAGGAGAAAGTGGAATCTTTGGAACAGGAAGCAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCT
CAATGACCGCCGCCGCCTGGCCCTGGAGAACTACATCACCGCTCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTC
CGCGCAGAACAGAAGGACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCAGATCCGGTCCCAGGTTATGA
CACACCTCCGTGTGATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCAGGATGAAGTTGGTGCAGT
GGCTCATGCCTGTAATTCCAGCATTTTGGGAGGCCAAGGTGGGCAGATGACTTGAGCCCAGAAGTTCAAGACCAGAATGGGAAACATGGCAAGACCACAT
TTCTACAAAAAAATTATCCAGGCATGATAACATCTATTTGTAGTCCCAGCTACTCAGGAGGCTGTGGTGGGAGGATCTCCTGAGCCTGGGGTGGCTGAGG
CTGCAGTGAGCCTTGATCACGCCACCTGGGCAATAGAGCAAGACCCTGTCTCAAAAAAAGGAAGAAAAAGACTATTATTTCCCCCATTGAATGGTCTTGG
CACTATTACACAAAATCAATTGTCCATAGATAATATGGGTTTATTTCTTAATTCTTAGTTCTTTTCTTTGATCTGTGTGCCTGTGCTTACTGTAGTACCA
CACTGTTTTGATTATTGTAGCTTTGTAGTAAATTTTGAAATCAGCAAGTGTGAGTCCGTTATCTTTGTTCTTCTTTTTCAATATTACTTTGAGTATCCGG
AGTCTCTTGCATTACAACATGAATTTTAAGATCTTCC
  • APP.F7 (forward primer)
  • APP.R2 (reverse primer)
  • Single exon skipping

Frame 0

LQITNVVEANQPVTIQNWCKRGRKQCKTHPHFV
IPYRCLVGEFVSDALLVPDKCKFLHQERMDVCET
HLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKF
RGVEFVCCPLAEESDNVDSADAEEDDSDVWWGG
ADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDE
DDEDGDEVEEEAEEPYEEATERTTSIATTTTTT
TESVEEVVREVCSEQAETGPCRAMISRWYFDVT
EGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAIP
TTAASTPDAVDKYLETPGDENEHAHFQKAKERL
EAKHRERMSQVMREWEEAERQAKNLPKADKKAV
IQHFQEKVESLEQEAANERQQLVETHMARVEAML
NDRRRLALENYITALQAVPPRPRHVFNMLKKYV
RAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVM
THLRVIYERMNQSLSLLYNVPAVAEEIQDEVGAV
AHACNSSILGGQGGQMT*AQKFKTRMGNMARPH
FYKKIIQA**HLFVVPATQEAVVGGSPEPGVAE
AAVSLDHATWAIEQDPVSKKGRKRLLFPPLNGLG
TITQNQLSIDNMGLFLNS*FFSLICVPVLTVVP
HCFDYCSFVVNFEISKCESVIFVLLFQYYFEYP
ESLALQHEF*DL

Frame 1

CRSPMW*KPTNQ*PSRTGASGAASSARPIPTL*
FPTAA*LVSL*VMPFSFLTSANSYTRRGWMFAK
LIFTGTPSPKRHAVRRVPTCMTTACCCPAELTSS
EG*SLCVAHWLKKVTMWILLMRRRMTRMSGGAE
QTQTMQMGVKTK**K*QRRKKWLRWKKKKPMMT
RTMRMVMR*RKRLRNPTKKPQREPPALPPPPPPP
QSLWKRWFERCALNKPRRGRAEQ*SPAGTLM*L
KGSVPHSFTADVAATGTTLTQKSTAWPCVAAPF
LQQQPVPLMPLTSISRHLGMRMNMPISRKPKRGL
RPSTERECPRS*ENGKRQNVKQRTCLKLIRRQL
SSISRRKWNLWNRKQPTRDSSWWRHTWPEWKPC
SMTAAAWPWRTTSPLCRLFLLGLVTCSIC*RSMS
AQNRRTDSTP*SISSMCAWWIPRKPLRSGPRL*
HTSV*FMSA*ISLSPCSTTCLQWPRRFRMKLVQ
WLMPVIPAFWEAKVGR*LEPRSSRPEWETWQDHI
STKKLSRHDNIYL*SQLLRRLWWEDLLSLGWLR
LQ*ALITPPGQ*SKTLSQKKEEKDYYFPH*MVL
ALLHKINCP*IIWVYFLILSSFL*SVCLCLL*YH
TVLIIVAL**ILKSASVSPLSLFFFFNITLSIR
SLLHYNMNFKIF

Frame 2

ADHQCGRSQPTSDHPELVQAGPQAVQDPSPLCD
SLPLLSW*VCK*CPSRS*QVQILTPGEDGCLRN
SSSLAHRRQRDMQ*EEYQLA*LRHVAALRN*QV
PRGRVCVLPTG*RK*QCGFC*CGGG*LGCLVGRS
RHRLCRWE*RQSSRSSRGGRSG*GGRRRSR**R
GR*GW**GRGRG*GTLRRSHRENHQHCHHHHHH
HRVCGRGGSRGVL*TSRDGAVPSNDLPLVL*CD*
REVCPILLRRMWRQPEQL*HRRVLHGRVWQRHS
YNSSQYP*CR*QVSRDTWG*E*TCPFPESQREA
*GQAPRENVPGHERMGRGRTSSKELA*S**EGSY
PAFPGESGIFGTGSSQRETAAGGDTHGQSGSHA
Q*PPPPGPGELHHRSAGCSSSASSRVQYAKEVC
PRRTEGQTAHPKAFRACAHGGSQESRSDPVPGYD
TPPCDL*AHESVSLPALQRACSGRGDSG*SWCS
GSCL*FQHFGRPRWADDLSPEVQDQNGKHGKTT
FLQKNYPGMITSICSPSYSGGCGGRIS*AWGG*G
CSEP*SRHLGNRARPCLKKRKKKTIISPIEWSW
HYYTKSIVHR*YGFIS*FLVLFFDLCACAYCST
TLF*LL*LCSKF*NQQV*VRYLCSSFSILL*VSG
VSCITT*ILRSS

Sequence APP.bAug05

GGATCAGCTGACTCGCCTGGCTCTGAGCCCCGCCGCCGCGCTCGGGCTCCGTCAGTTTCCTCGGCAGCGGTAGGCGAGAGCACGCGGAGGAGCGTGCGCG
GGGGCCCCGGGAGACGGCGGCGGTGGCGGCGCGGGCAGAGCAAGGACGCGGCGGATCCCACTCGCACAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCG
ATGCTGCCCGGTTTGGCACTGCTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGAGGTACCCACTGATGGTAATGCTGGCCTGCTGGCTGAACCCCAGA
TTGCCATGTTCTGTGGCAGACTGAACATGCACATGAATGTCCAGAATGGGAAGTGGGATTCAGATCCATCAGGGACCAAAACCTGCATTGATACCAAGGA
AGGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTGCAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGG
GGCCGCAAGCAGTGCAAGACCCATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCA
AATTCTTACACCAGGAGAGGATGGATGTTTGCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAACTTGCATGA
CTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTCCGAGGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGCTGAT
GCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACTATGCAGATGGGAGTGAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAG
TGGCTGAGGTGGAAGAAGAAGAAGCCGATGATGACGAGGACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGAGGAACCCTACGAAGAAGCCACAGA
GAGAACCACCAGCATTGCCACCACCACCACCACCACCACAGAGTCTGTGGAAGAGGTGGTTCGAGAGGTGTGCTCTGAACAAGCCGAGACGGGGCCGTGC
CGAGCAATGATCTCCCGCTGGTACTTTGATGTGACTGAAGGGAAGTGTGCCCCATTCTTTTACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAG
AAGAGTACTGCATGGCCGTGTGTGGCAGCGCCATTCCTACAACAGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCGAGACACCTGGGGATGAGAA
TGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAAGAGGCAGAACGTCAA
GCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGCATTTCCAGGAGAAAGTGGAATCTTTGGAACAGGAAGCAGCCAACGAGAGACAGCAGC
TGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCTCAATGACCGCCGCCGCCTGGCCCTGGAGAACTACATCACCGCTCTGCAGGCTGTTCCTCCTCG
GCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCGCGCAGAACAGAAGGACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGAT
CCCAAGAAAGCCGCTCAGATCCGGTCCCAGGTTATGACACACCTCCGTGTGATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTG
CAGTGGCCGAGGAGATTCAGGATGAAGTTGATGAGCTGCTTCAGAAAGAGCAAAACTATTCAGATGACGTCTTGGCCAACATGATTAGTGAACCAAGGAT
CAGTTACGGAAACGATGCTCTCATGCCATCTTTGACCGAAACGAAAACCACCGTGGAGCTCCTTCCCGTGAATGGAGAGTTCAGCCTGGACGATCTCCAG
CCGTGGCATTCTTTTGGGGCTGACTCTGTGCCAGCCAACACAGAAAACGAAGTTGAGCCTGTTGATGCCCGCCCTGCTGCCGACCGAGGACTGACCACTC
GACCAGGTTCTGGGTTGACAAATATCAAGACGGAGGAGATCTCTGAAGTGAAGATGGATGCAGAATTCCGACATGACTCAGGATATGAAGTTCATCATCA
AAAATTGGTGTTCTTTGCAGAAGATGTGGGTTCAAACAAAGGTGCAATCATTGGACTCATGGTGGGCGGTGTTGTCATAGCGACAGTGATCGTCATCACC
TTGGTGATGCTGAAGAAGAAACAGTACACATCCATTCATCATGGTGTGGTGGAGGTTGACGCCGCTGTCACCCCAGAGGAGCGCCACCTGTCCAAGATGC
AGCAGAACGGCTACGAAAATCCAACCTACAAGTTCTTTGAGCAGATGCAGAACTAGACCCCCGCCACAGCAGCCTCTGAAGTTGGACAGCAAAACCATTG
CTTCACTACCCATCGGTGTCCATTTATAGAATAATGTGGGAAGAAACAAACCCGTTTTATGATTTACTCATTATCGCCTTTTGACAGCTGTGCTGTAACA
CAAGTAGATGCCTGAACTTGAATTAATCCACACATCAGTAATGTATTCTATCTCTCTTTACATTTTGGTCTCTATACTACATTATTAATGGGTTTTGTGT
ACTGTAAAGAATTTAGCTGTATCAAACTAGTGCATGAATAGATTCTCTCCTGATTATTTATCACATAGCCCCTTAGCCAGTTGTATATTATTCTTGTGGT
TTGTGACCCAATTAAGTCCTACTTTACATATGCTTTAAGAATCGATGGGGGATGCTTCATGTGAACGTGGGAGTTCAGCTGCTTCTCTTGCCTAAGTATT
CCTTTCCTGATCACTATGCATTTTAAAGTTAAACATTTTTAAGTATTTCAGATGCTTTAGAGAGATTTTTTTTCCATGACTGCATTTTACTGTACAGATT
GCTGCTTCTGCTATATTTGTGATATAGGAATTAAGAGGATACACACGTTTGTTTCTTCGTGCCTGTTTTATGTGCACACATTAGGCATTGAGACTTCAAG
CTTTTCTTTTTTTGTCCACGTATCTTTGGGTCTTTGATAAAGAAAAGAATCCCTGTTCATTGTAAGCACTTTTACGGGGCGGGTGGGGAGGGGTGCTCTG
CTGGTCTTCAATTACCAAGAATTCTCCAAAACAATTTTCTGCAGGATGATTGTACAGAATCATTGCTTATGACATGATCGCTTTCTACACTGTATTACAT
AAATAAATTAAATAAAATAACCCCGGGCAAGACTTTTCTTTGAAGGATGACTACAGACATTAAATAATCGAAGTAATTTTGGGTGGGGAGAAGAGGCAGA
TTCAATTTTCTTTAACCAGTCTGAAGTTTCATTTATGATACAAAAGAAGATGAAAATGGAAGTGGCAATATAAGGGGATGAGGAAGGCATGCCTGGACAA
ACCCTTCTTTTAAGATGTGTCTTCAATTTGTATAAAATGGTGTTTTCATGTAAATAAATACATTCTTGGAGGAGCACCAT
  • APP.F7 (forward primer)
  • APP.R2 (reverse primer)
  • Single exon skipping

Frame 0

GSADSPGSEPRRRARAPSVSSAAVGESTRRSVR
GGPGRRRRWRRGQSKDAADPTRTAAHSVPRAGSR
CCPVWHCSCWPPGRLGRWRYPLMVMLACWLNPR
LPCSVAD*TCT*MSRMGSGIQIHQGPKPALIPR
KASCSIAKKSTLNCRSPMW*KPTNQ*PSRTGASG
AASSARPIPTL*FPTAA*LVSL*VMPFSFLTSA
NSYTRRGWMFAKLIFTGTPSPKRHAVRRVPTCM
TTACCCPAELTSSEG*SLCVAHWLKKVTMWILLM
RRRMTRMSGGAEQTQTMQMGVKTK**K*QRRKK
WLRWKKKKPMMTRTMRMVMR*RKRLRNPTKKPQ
REPPALPPPPPPPQSLWKRWFERCALNKPRRGRA
EQ*SPAGTLM*LKGSVPHSFTADVAATGTTLTQ
KSTAWPCVAAPFLQQQPVPLMPLTSISRHLGMR
MNMPISRKPKRGLRPSTERECPRS*ENGKRQNVK
QRTCLKLIRRQLSSISRRKWNLWNRKQPTRDSS
WWRHTWPEWKPCSMTAAAWPWRTTSPLCRLFLL
GLVTCSIC*RSMSAQNRRTDSTP*SISSMCAWWI
PRKPLRSGPRL*HTSV*FMSA*ISLSPCSTTCL
QWPRRFRMKLMSCFRKSKTIQMTSWPT*LVNQG
SVTETMLSCHL*PKRKPPWSSFP*MESSAWTISS
RGILLGLTLCQPTQKTKLSLLMPALLPTED*PL
DQVLG*QISRRRRSLK*RWMQNSDMTQDMKFII
KNWCSLQKMWVQTKVQSLDSWWAVLS*RQ*SSSP
W*C*RRNSTHPFIMVWWRLTPLSPQRSATCPRC
SRTATKIQPTSSLSRCRTRPPPQQPLKLDSKTI
ASLPIGVHL*NNVGRNKPVL*FTHYRLLTAVL*H
K*MPELELIHTSVMYSISLYILVSILHY*WVLC
TVKNLAVSN*CMNRFSPDYLSHSPLASCILFLW
FVTQLSPTLHML*ESMGDASCERGSSAASLA*VF
LS*SLCILKLNIFKYFRCFREIFFP*LHFTVQI
AASAIFVI*ELRGYTRLFLRACFMCTH*ALRLQ
AFLFLSTYLWVFDKEKNPCSL*ALLRGGWGGVLC
WSSITKNSPKQFSAG*LYRIIAYDMIAFYTVLH
K*IK*NNPGQDFSLKDDYRH*IIEVILGGEKRQ
IQFSLTSLKFHL*YKRR*KWKWQYKGMRKACLDK
PFF*DVSSICIKWCFHVNKYILGGAP

Frame 1

DQLTRLALSPAAALGLRQFPRQR*ARARGGACA
GAPGDGGGGGAGRARTRRIPLAQQRTRCPAQGR
DAARFGTAPAGRLDGSGAGGTH*W*CWPAG*TPD
CHVLWQTEHAHECPEWEVGFRSIRDQNLH*YQG
RHPAVLPRSLP*TADHQCGRSQPTSDHPELVQA
GPQAVQDPSPLCDSLPLLSW*VCK*CPSRS*QVQ
ILTPGEDGCLRNSSSLAHRRQRDMQ*EEYQLA*
LRHVAALRN*QVPRGRVCVLPTG*RK*QCGFC*
CGGG*LGCLVGRSRHRLCRWE*RQSSRSSRGGRS
G*GGRRRSR**RGR*GW**GRGRG*GTLRRSHR
ENHQHCHHHHHHHRVCGRGGSRGVL*TSRDGAV
PSNDLPLVL*CD*REVCPILLRRMWRQPEQL*HR
RVLHGRVWQRHSYNSSQYP*CR*QVSRDTWG*E
*TCPFPESQREA*GQAPRENVPGHERMGRGRTS
SKELA*S**EGSYPAFPGESGIFGTGSSQRETAA
GGDTHGQSGSHAQ*PPPPGPGELHHRSAGCSSS
ASSRVQYAKEVCPRRTEGQTAHPKAFRACAHGG
SQESRSDPVPGYDTPPCDL*AHESVSLPALQRAC
SGRGDSG*S**AASERAKLFR*RLGQHD**TKD
QLRKRCSHAIFDRNENHRGAPSREWRVQPGRSP
AVAFFWG*LCASQHRKRS*AC*CPPCCRPRTDHS
TRFWVDKYQDGGDL*SEDGCRIPT*LRI*SSSS
KIGVLCRRCGFKQRCNHWTHGGRCCHSDSDRHH
LGDAEEETVHIHSSWCGGG*RRCHPRGAPPVQDA
AERLRKSNLQVL*ADAELDPRHSSL*SWTAKPL
LHYPSVSIYRIMWEETNPFYDLLIIAF*QLCCN
TSRCLNLN*STHQ*CILSLFTFWSLYYIINGFCV
L*RI*LYQTSA*IDSLLIIYHIAP*PVVYYSCG
L*PN*VLLYICFKNRWGMLHVNVGVQLLLLPKY
SFPDHYAF*S*TFLSISDALERFFFHDCILLYRL
LLLLYL*YRN*EDTHVCFFVPVLCAHIRH*DFK
LFFFCPRIFGSLIKKRIPVHCKHFYGAGGEGCS
AGLQLPRILQNNFLQDDCTESLLMT*SLSTLYYI
NKLNKITPGKTFL*RMTTDIK*SK*FWVGRRGR
FNFL*PV*SFIYDTKEDENGSGNIRG*GRHAWT
NPSFKMCLQFV*NGVFM*INTFLEEHH

Frame 2

IS*LAWL*APPPRSGSVSFLGSGRREHAEERAR
GPRETAAVAARAEQGRGGSHSHSSALGAPRRVA
MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQ
IAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKE
GILQYCQEVYPELQITNVVEANQPVTIQNWCKR
GRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKC
KFLHQERMDVCETHLHWHTVAKETCSEKSTNLHD
YGMLLPCGIDKFRGVEFVCCPLAEESDNVDSAD
AEEDDSDVWWGGADTDYADGSEDKVVEVAEEEE
VAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATE
RTTSIATTTTTTTESVEEVVREVCSEQAETGPC
RAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDT
EEYCMAVCGSAIPTTAASTPDAVDKYLETPGDEN
EHAHFQKAKERLEAKHRERMSQVMREWEEAERQ
AKNLPKADKKAVIQHFQEKVESLEQEAANERQQ
LVETHMARVEAMLNDRRRLALENYITALQAVPPR
PRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVD
PKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVP
AVAEEIQDEVDELLQKEQNYSDDVLANMISEPRI
SYGNDALMPSLTETKTTVELLPVNGEFSLDDLQ
PWHSFGADSVPANTENEVEPVDARPAADRGLTT
RPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQ
KLVFFAEDVGSNKGAIIGLMVGGVVIATVIVIT
LVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKM
QQNGYENPTYKFFEQMQN*TPATAASEVGQQNHC
FTTHRCPFIE*CGKKQTRFMIYSLSPFDSCAVT
QVDA*T*INPHISNVFYLSLHFGLYTTLLMGFV
YCKEFSCIKLVHE*ILS*LFIT*PLSQLYIILVV
CDPIKSYFTYALRIDGGCFM*TWEFSCFSCLSI
PFLITMHFKVKHF*VFQML*RDFFSMTAFYCTD
CCFCYICDIGIKRIHTFVSSCLFYVHTLGIETSS
FSFFVHVSLGL**RKESLFIVSTFTGRVGRGAL
LVFNYQEFSKTIFCRMIVQNHCL*HDRFLHCIT
*IN*IK*PRARLFFEG*LQTLNNRSNFGWGEEAD
SIFFNQSEVSFMIQKKMKMEVAI*GDEEGMPGQ
TLLLRCVFNLYKMVFSCK*IHSWRST

Sequence APP.aAug05

GGATCAGCTGACTCGCCTGGCTCTGAGCCCCGCCGCCGCGCTCGGGCTCCGTCAGTTTCCTCGGCAGCGGTAGGCGAGAGCACGCGGAGGAGCGTGCGCG
GGGGCCCCGGGAGACGGCGGCGGTGGCGGCGCGGGCAGAGCAAGGACGCGGCGGATCCCACTCGCACAGCAGCGCACTCGGTGCCCCGCGCAGGGTCGCG
ATGCTGCCCGGTTTGGCACTGCTCCTGCTGGCCGCCTGGACGGCTCGGGCGCTGGAGGTACCCACTGATGGTAATGCTGGCCTGCTGGCTGAACCCCAGA
TTGCCATGTTCTGTGGCAGACTGAACATGCACATGAATGTCCAGAATGGGAAGTGGGATTCAGATCCATCAGGGACCAAAACCTGCATTGATACCAAGGA
AGGCATCCTGCAGTATTGCCAAGAAGTCTACCCTGAACTGCAGATCACCAATGTGGTAGAAGCCAACCAACCAGTGACCATCCAGAACTGGTGCAAGCGG
GGCCGCAAGCAGTGCAAGACCCATCCCCACTTTGTGATTCCCTACCGCTGCTTAGTTGGTGAGTTTGTAAGTGATGCCCTTCTCGTTCCTGACAAGTGCA
AATTCTTACACCAGGAGAGGATGGATGTTTGCGAAACTCATCTTCACTGGCACACCGTCGCCAAAGAGACATGCAGTGAGAAGAGTACCAACTTGCATGA
CTACGGCATGTTGCTGCCCTGCGGAATTGACAAGTTCCGAGGGGTAGAGTTTGTGTGTTGCCCACTGGCTGAAGAAAGTGACAATGTGGATTCTGCTGAT
GCGGAGGAGGATGACTCGGATGTCTGGTGGGGCGGAGCAGACACAGACTATGCAGATGGGAGTGAAGACAAAGTAGTAGAAGTAGCAGAGGAGGAAGAAG
TGGCTGAGGTGGAAGAAGAAGAAGCCGATGATGACGAGGACGATGAGGATGGTGATGAGGTAGAGGAAGAGGCTGAGGAACCCTACGAAGAAGCCACAGA
GAGAACCACCAGCATTGCCACCACCACCACCACCACCACAGAGTCTGTGGAAGAGGTGGTTCGAGAGGTGTGCTCTGAACAAGCCGAGACGGGGCCGTGC
CGAGCAATGATCTCCCGCTGGTACTTTGATGTGACTGAAGGGAAGTGTGCCCCATTCTTTTACGGCGGATGTGGCGGCAACCGGAACAACTTTGACACAG
AAGAGTACTGCATGGCCGTGTGTGGCAGCGCCATGTCCCAAAGTTTACTCAAGACTACCCAGGAACCTCTTGCCCGAGATCCTGTTAAACTTCCTACAAC
AGCAGCCAGTACCCCTGATGCCGTTGACAAGTATCTCGAGACACCTGGGGATGAGAATGAACATGCCCATTTCCAGAAAGCCAAAGAGAGGCTTGAGGCC
AAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAAGAGGCAGAACGTCAAGCAAAGAACTTGCCTAAAGCTGATAAGAAGGCAGTTATCCAGC
ATTTCCAGGAGAAAGTGGAATCTTTGGAACAGGAAGCAGCCAACGAGAGACAGCAGCTGGTGGAGACACACATGGCCAGAGTGGAAGCCATGCTCAATGA
CCGCCGCCGCCTGGCCCTGGAGAACTACATCACCGCTCTGCAGGCTGTTCCTCCTCGGCCTCGTCACGTGTTCAATATGCTAAAGAAGTATGTCCGCGCA
GAACAGAAGGACAGACAGCACACCCTAAAGCATTTCGAGCATGTGCGCATGGTGGATCCCAAGAAAGCCGCTCAGATCCGGTCCCAGGTTATGACACACC
TCCGTGTGATTTATGAGCGCATGAATCAGTCTCTCTCCCTGCTCTACAACGTGCCTGCAGTGGCCGAGGAGATTCAGGATGAAGTTGATGAGCTGCTTCA
GAAAGAGCAAAACTATTCAGATGACGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATGCTCTCATGCCATCTTTGACCGAAACG
AAAACCACCGTGGAGCTCCTTCCCGTGAATGGAGAGTTCAGCCTGGACGATCTCCAGCCGTGGCATTCTTTTGGGGCTGACTCTGTGCCAGCCAACACAG
AAAACGAAGTTGAGCCTGTTGATGCCCGCCCTGCTGCCGACCGAGGACTGACCACTCGACCAGGTTCTGGGTTGACAAATATCAAGACGGAGGAGATCTC
TGAAGTGAAGATGGATGCAGAATTCCGACATGACTCAGGATATGAAGTTCATCATCAAAAATTGGTGTTCTTTGCAGAAGATGTGGGTTCAAACAAAGGT
GCAATCATTGGACTCATGGTGGGCGGTGTTGTCATAGCGACAGTGATCGTCATCACCTTGGTGATGCTGAAGAAGAAACAGTACACATCCATTCATCATG
GTGTGGTGGAGGTTGACGCCGCTGTCACCCCAGAGGAGCGCCACCTGTCCAAGATGCAGCAGAACGGCTACGAAAATCCAACCTACAAGTTCTTTGAGCA
GATGCAGAACTAGACCCCCGCCACAGCAGCCTCTGAAGTTGGACAGCAAAACCATTGCTTCACTACCCATCGGTGTCCATTTATAGAATAATGTGGGAAG
AAACAAACCCGTTTTATGATTTACTCATTATCGCCTTTTGACAGCTGTGCTGTAACACAAGTAGATGCCTGAACTTGAATTAATCCACACATCAGTAATG
TATTCTATCTCTCTTTACATTTTGGTCTCTATACTACATTATTAATGGGTTTTGTGTACTGTAAAGAATTTAGCTGTATCAAACTAGTGCATGAATAGAT
TCTCTCCTGATTATTTATCACATAGCCCCTTAGCCAGTTGTATATTATTCTTGTGGTTTGTGACCCAATTAAGTCCTACTTTACATATGCTTTAAGAATC
GATGGGGGATGCTTCATGTGAACGTGGGAGTTCAGCTGCTTCTCTTGCCTAAGTATTCCTTTCCTGATCACTATGCATTTTAAAGTTAAACATTTTTAAG
TATTTCAGATGCTTTAGAGAGATTTTTTTTCCATGACTGCATTTTACTGTACAGATTGCTGCTTCTGCTATATTTGTGATATAGGAATTAAGAGGATACA
CACGTTTGTTTCTTCGTGCCTGTTTTATGTGCACACATTAGGCATTGAGACTTCAAGCTTTTCTTTTTTTGTCCACGTATCTTTGGGTCTTTGATAAAGA
AAAGAATCCCTGTTCATTGTAAGCACTTTTACGGGGCGGGTGGGGAGGGGTGCTCTGCTGGTCTTCAATTACCAAGAATTCTCCAAAACAATTTTCTGCA
GGATGATTGTACAGAATCATTGCTTATGACATGATCGCTTTCTACACTGTATTACATAAATAAATTAAATAAAATAACCCCGGGCAAGACTTTTCTTTGA
AGGATGACTACAGACATTAAATAATCGAAGTAATTTTGGGTGGGGAGAAGAGGCAGATTCAATTTTCTTTAACCAGTCTGAAGTTTCATTTATGATACAA
AAGAAGATGAAAATGGAAGTGGCAATATAAGGGGATGAGGAAGGCATGCCTGGACAAACCCTTCTTTTAAGATGTGTCTTCAATTTGTATAAAATGGTGT
TTTCATGTAAATAAATACATTCTTGGAGGAGCACCAT
  • APP.F7 (forward primer)
  • APP.R2 (reverse primer)
  • Single exon skipping

Frame 0

GSADSPGSEPRRRARAPSVSSAAVGESTRRSVR
GGPGRRRRWRRGQSKDAADPTRTAAHSVPRAGSR
CCPVWHCSCWPPGRLGRWRYPLMVMLACWLNPR
LPCSVAD*TCT*MSRMGSGIQIHQGPKPALIPR
KASCSIAKKSTLNCRSPMW*KPTNQ*PSRTGASG
AASSARPIPTL*FPTAA*LVSL*VMPFSFLTSA
NSYTRRGWMFAKLIFTGTPSPKRHAVRRVPTCM
TTACCCPAELTSSEG*SLCVAHWLKKVTMWILLM
RRRMTRMSGGAEQTQTMQMGVKTK**K*QRRKK
WLRWKKKKPMMTRTMRMVMR*RKRLRNPTKKPQ
REPPALPPPPPPPQSLWKRWFERCALNKPRRGRA
EQ*SPAGTLM*LKGSVPHSFTADVAATGTTLTQ
KSTAWPCVAAPCPKVYSRLPRNLLPEILLNFLQ
QQPVPLMPLTSISRHLGMRMNMPISRKPKRGLRP
STERECPRS*ENGKRQNVKQRTCLKLIRRQLSS
ISRRKWNLWNRKQPTRDSSWWRHTWPEWKPCSM
TAAAWPWRTTSPLCRLFLLGLVTCSIC*RSMSAQ
NRRTDSTP*SISSMCAWWIPRKPLRSGPRL*HT
SV*FMSA*ISLSPCSTTCLQWPRRFRMKLMSCF
RKSKTIQMTSWPT*LVNQGSVTETMLSCHL*PKR
KPPWSSFP*MESSAWTISSRGILLGLTLCQPTQ
KTKLSLLMPALLPTED*PLDQVLG*QISRRRRS
LK*RWMQNSDMTQDMKFIIKNWCSLQKMWVQTKV
QSLDSWWAVLS*RQ*SSSPW*C*RRNSTHPFIM
VWWRLTPLSPQRSATCPRCSRTATKIQPTSSLS
RCRTRPPPQQPLKLDSKTIASLPIGVHL*NNVGR
NKPVL*FTHYRLLTAVL*HK*MPELELIHTSVM
YSISLYILVSILHY*WVLCTVKNLAVSN*CMNR
FSPDYLSHSPLASCILFLWFVTQLSPTLHML*ES
MGDASCERGSSAASLA*VFLS*SLCILKLNIFK
YFRCFREIFFP*LHFTVQIAASAIFVI*ELRGY
TRLFLRACFMCTH*ALRLQAFLFLSTYLWVFDKE
KNPCSL*ALLRGGWGGVLCWSSITKNSPKQFSA
G*LYRIIAYDMIAFYTVLHK*IK*NNPGQDFSL
KDDYRH*IIEVILGGEKRQIQFSLTSLKFHL*YK
RR*KWKWQYKGMRKACLDKPFF*DVSSICIKWC
FHVNKYILGGAP

Frame 1

DQLTRLALSPAAALGLRQFPRQR*ARARGGACA
GAPGDGGGGGAGRARTRRIPLAQQRTRCPAQGR
DAARFGTAPAGRLDGSGAGGTH*W*CWPAG*TPD
CHVLWQTEHAHECPEWEVGFRSIRDQNLH*YQG
RHPAVLPRSLP*TADHQCGRSQPTSDHPELVQA
GPQAVQDPSPLCDSLPLLSW*VCK*CPSRS*QVQ
ILTPGEDGCLRNSSSLAHRRQRDMQ*EEYQLA*
LRHVAALRN*QVPRGRVCVLPTG*RK*QCGFC*
CGGG*LGCLVGRSRHRLCRWE*RQSSRSSRGGRS
G*GGRRRSR**RGR*GW**GRGRG*GTLRRSHR
ENHQHCHHHHHHHRVCGRGGSRGVL*TSRDGAV
PSNDLPLVL*CD*REVCPILLRRMWRQPEQL*HR
RVLHGRVWQRHVPKFTQDYPGTSCPRSC*TSYN
SSQYP*CR*QVSRDTWG*E*TCPFPESQREA*G
QAPRENVPGHERMGRGRTSSKELA*S**EGSYPA
FPGESGIFGTGSSQRETAAGGDTHGQSGSHAQ*
PPPPGPGELHHRSAGCSSSASSRVQYAKEVCPR
RTEGQTAHPKAFRACAHGGSQESRSDPVPGYDTP
PCDL*AHESVSLPALQRACSGRGDSG*S**AAS
ERAKLFR*RLGQHD**TKDQLRKRCSHAIFDRN
ENHRGAPSREWRVQPGRSPAVAFFWG*LCASQHR
KRS*AC*CPPCCRPRTDHSTRFWVDKYQDGGDL
*SEDGCRIPT*LRI*SSSSKIGVLCRRCGFKQR
CNHWTHGGRCCHSDSDRHHLGDAEEETVHIHSSW
CGGG*RRCHPRGAPPVQDAAERLRKSNLQVL*A
DAELDPRHSSL*SWTAKPLLHYPSVSIYRIMWE
ETNPFYDLLIIAF*QLCCNTSRCLNLN*STHQ*C
ILSLFTFWSLYYIINGFCVL*RI*LYQTSA*ID
SLLIIYHIAP*PVVYYSCGL*PN*VLLYICFKN
RWGMLHVNVGVQLLLLPKYSFPDHYAF*S*TFLS
ISDALERFFFHDCILLYRLLLLLYL*YRN*EDT
HVCFFVPVLCAHIRH*DFKLFFFCPRIFGSLIK
KRIPVHCKHFYGAGGEGCSAGLQLPRILQNNFLQ
DDCTESLLMT*SLSTLYYINKLNKITPGKTFL*
RMTTDIK*SK*FWVGRRGRFNFL*PV*SFIYDT
KEDENGSGNIRG*GRHAWTNPSFKMCLQFV*NGV
FM*INTFLEEHH

Frame 2

IS*LAWL*APPPRSGSVSFLGSGRREHAEERAR
GPRETAAVAARAEQGRGGSHSHSSALGAPRRVA
MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQ
IAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKE
GILQYCQEVYPELQITNVVEANQPVTIQNWCKR
GRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKC
KFLHQERMDVCETHLHWHTVAKETCSEKSTNLHD
YGMLLPCGIDKFRGVEFVCCPLAEESDNVDSAD
AEEDDSDVWWGGADTDYADGSEDKVVEVAEEEE
VAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATE
RTTSIATTTTTTTESVEEVVREVCSEQAETGPC
RAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDT
EEYCMAVCGSAMSQSLLKTTQEPLARDPVKLPTT
AASTPDAVDKYLETPGDENEHAHFQKAKERLEA
KHRERMSQVMREWEEAERQAKNLPKADKKAVIQ
HFQEKVESLEQEAANERQQLVETHMARVEAMLND
RRRLALENYITALQAVPPRPRHVFNMLKKYVRA
EQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTH
LRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQ
KEQNYSDDVLANMISEPRISYGNDALMPSLTET
KTTVELLPVNGEFSLDDLQPWHSFGADSVPANT
ENEVEPVDARPAADRGLTTRPGSGLTNIKTEEIS
EVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKG
AIIGLMVGGVVIATVIVITLVMLKKKQYTSIHH
GVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQ
MQN*TPATAASEVGQQNHCFTTHRCPFIE*CGK
KQTRFMIYSLSPFDSCAVTQVDA*T*INPHISN
VFYLSLHFGLYTTLLMGFVYCKEFSCIKLVHE*I
LS*LFIT*PLSQLYIILVVCDPIKSYFTYALRI
DGGCFM*TWEFSCFSCLSIPFLITMHFKVKHF*
VFQML*RDFFSMTAFYCTDCCFCYICDIGIKRIH
TFVSSCLFYVHTLGIETSSFSFFVHVSLGL**R
KESLFIVSTFTGRVGRGALLVFNYQEFSKTIFC
RMIVQNHCL*HDRFLHCIT*IN*IK*PRARLFFE
G*LQTLNNRSNFGWGEEADSIFFNQSEVSFMIQ
KKMKMEVAI*GDEEGMPGQTLLLRCVFNLYKMV
FSCK*IHSWRST

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