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Sequences

Sequence EPGN.cAug05

GACAGCACTGACCGAAGAGGCAGCCGTGACTGTAACACCTCCAATCACAGCCCAGCAAGCTGACAACATAGAAGGACCCATAGCCTTGAAGTTCTCACAC CTTTGCCTGGAAGATCATAACAGTTACTGCATCAACGGTGCTTGTGCATTCCACCATGAGCTAGAGAAAGCCATCTGCAGGTGTTTTACTGGTTATACTG GAGAAAGGTGTGAGCACTTGACTTTAACTTCATATGCTGTGGATTCTTATGAAAAATACATTGCAATTGGGATTGGTGTTGGATTACTATTAAGTGGTTT TCTTGTTATTTTTTACTGCTATATAAGAAAGAGGTGTCTAAAATTGAAATCGCCTTACAATGTCTGTTCTGGAGAAAGACGACCACTGTGAGGCCTTTGT GAAGAATTTTCATCAAGGCATCTGTAGAGATCAGTGAGCCCAAAATTAAAGTTTTCAGATGAAACAACAAAACTTGTCAAGCTGACTAGACTCGAAAATA ATGAAAGTTGGGATCACAATGAAATGAGAAGATAAAATTCAGCGTTGGCCTTTAGACTTTGCCATCCTTAAGGAGTGATGGAAGCCAAGTGAACAAGCCT CAGTGACACAAGTCAAATTCATAGTTTCACTCTGGGTTTTTTGTTGTTGTGTGGTTATTATTCTCACTACAGAAAGACTGAGTTTCATGCTCCTGGCTAT GTCAGATGTGAATTTTCATGGGAATAATAATCAACCTTGCAGCAAGCCAAAGCAATGCCTCGCTTGGGTTCTTCATGTTCTTACTACCCAGCGTTTTTTA CCACCTAGATGGGCCTCTCTAAGTCTATTTGCTCAATGAACCTTATCCCAAACTTGTTGTTTTCATGGTGTCTGAAAGAATGTGATGTCAGCTTTTAGAA TGAAATCAGTGTTAAGGTACCTCCAGTGAACCAAACATGTGTCTTAAATCTAAGCCACTGAAAACAGAAGGGAATGTCCAAGGCAAATACAAATCATACA CAGCTTGTAACAACATACAGCCTTTCTATGTGAAATAATGGAATAAACTAAAGAGTTTCTGAAGACTGAAGCTATCTGGATATCAAGTCTGGAGTAGGCA AAGCATTTCCATTTCTACATGGATTATAAAACTTTGTGTTGGACTCATTGGTCCCTAATGCTTTTGTTCATCACTTCTTCCAGTTATCAGTGGAATTACC TGGTGACCATTCATTTGGACCGAAATCCTGGAAGTCTCCTACTGAATAAAAGTCTACAATTGGCCCTAAAATAGAAACTGAAAAACAGGACATAGAATTT TTTCACCAGACCACAGCATGTGGAAACTTTCTTTATCATTTTTGAACACTTGTTAACAGATTTGCACATAGAGGGAGAGAAAAAAAATGGAGTAACAGTC AAAATAATAATAATCAGTATCCAGGCCAGGCGTGGTGGTTCACGCCTGTAATCCTAGCACTTTGGGAGGCCGAGGCGGATGGAGCACCTGAGGTCAGGAG TTTGAGACCAGCCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCTGGGCGTGGTGGTGCATGCCTGTAATCCCAGCTACTCCA GAGGCTGACACAGGACAATCACTTGAACCCAGGAGGCAGAGGTTGTAGTGAGCCGAGATGGCAACCCTGCACTCCAACCTGGGTGACAAGAGCGAAACTC CATCTCAAAAAAAAAAAAAAAAA

EPGN.F3 (forward primer)
EPGN.R4 (reverse primer)
Alternative donor

Frame 0

DSTDRRGSRDCNTSNHSPAS*QHRRTHSLEVLT PLPGRS*QLLHQRCLCIPP*ARESHLQVFYWLYW RKV*ALDFNFICCGFL*KIHCNWDWCWITIKWF SCYFLLLYKKEVSKIEIALQCLFWRKTTTVRPL *RIFIKASVEISEPKIKVFR*NNKTCQAD*TRK* *KLGSQ*NEKIKFSVGL*TLPSLRSDGSQVNKP Q*HKSNS*FHSGFFVVVWLLFSLQKD*VSCSWL CQM*IFMGIIINLAASQSNASLGFFMFLLPSVFY HLDGPL*VYLLNEPYPKLVVFMVSERM*CQLLE *NQC*GTSSEPNMCLKSKPLKTEGNVQGKYKSY TACNNIQPFYVK*WNKLKSF*RLKLSGYQVWSRQ SISISTWIIKLCVGLIGP*CFCSSLLPVISGIT W*PFIWTEILEVSY*IKVYNWP*NRN*KTGHRI FSPDHSMWKLSLSFLNTC*QICT*RERKKME*QS K***SVSRPGVVVHACNPSTLGGRGGWST*GQE FETSLANMVKPCLY*KYKN*LGVVVHACNPSYS RG*HRTIT*TQEAEVVVSRDGNPALQPG*QERNS ISKKKKK

Frame 1

TALTEEAAVTVTPPITAQQADNIEGPIALKFSH LCLEDHNSYCINGACAFHHELEKAICRCFTGYT GERCEHLTLTSYAVDSYEKYIAIGIGVGLLLSGF LVIFYCYIRKRCLKLKSPYNVCSGERRPL*GLC EEFSSRHL*RSVSPKLKFSDETTKLVKLTRLEN NESWDHNEMRR*NSALAFRLCHP*GVMEAK*TSL SDTSQIHSFTLGFLLLCGYYSHYRKTEFHAPGY VRCEFSWE**STLQQAKAMPRLGSSCSYYPAFF TT*MGLSKSICSMNLIPNLLFSWCLKECDVSF*N EISVKVPPVNQTCVLNLSH*KQKGMSKANTNHT QLVTTYSLSM*NNGIN*RVSED*SYLDIKSGVG KAFPFLHGL*NFVLDSLVPNAFVHHFFQLSVELP GDHSFGPKSWKSPTE*KSTIGPKIETEKQDIEF FHQTTACGNFLYHF*TLVNRFAHRGREKKWSNS QNNNNQYPGQAWWFTPVILALWEAEADGAPEVRS LRPAWPTW*NPVSTKNTKISWAWWCMPVIPATP EADTGQSLEPRRQRL**AEMATLHSNLGDKSET PSQKKKKK

Frame 2

QH*PKRQP*L*HLQSQPSKLTT*KDP*P*SSHT FAWKIITVTASTVLVHSTMS*RKPSAGVLLVIL EKGVST*L*LHMLWILMKNTLQLGLVLDYY*VV FLLFFTAI*ERGV*N*NRLTMSVLEKDDHCEAFV KNFHQGICRDQ*AQN*SFQMKQQNLSS*LDSKI MKVGITMK*EDKIQRWPLDFAILKE*WKPSEQA SVTQVKFIVSLWVFCCCVVIILTTERLSFMLLAM SDVNFHGNNNQPCSKPKQCLAWVLHVLTTQRFL PPRWASLSLFAQ*TLSQTCCFHGV*KNVMSAFR MKSVLRYLQ*TKHVS*I*ATENRRECPRQIQIIH SL*QHTAFLCEIME*TKEFLKTEAIWISSLE*A KHFHFYMDYKTLCWTHWSLMLLFITSSSYQWNY LVTIHLDRNPGSLLLNKSLQLALK*KLKNRT*NF FTRPQHVETFFIIFEHLLTDLHIEGEKKNGVTV KIIIISIQARRGGSRL*S*HFGRPRRMEHLRSG V*DQPGQHGETLSLLKIQKLAGRGGACL*SQLLQ RLTQDNHLNPGGRGCSEPRWQPCTPTWVTRAKL HLKKKKK

Sequence EPGN.bAug05

EPGN.F3 (forward primer)
EPGN.R4 (reverse primer)
Alternative donor

Frame 0

NKNLPPVSLEG*EKES*ATTGNGFGSSNISLSF IQRNDSTDRRGSRDCNTSNHSPAS*QHRRTHSLE VLTPLPGRS*QLLHQRCLCIPP*ARESHLQVFY WLYWRKV*ALDFNFICCGFL*KIHCNWDWCWIT IKWFSCYFLLLYKKEV*KRQNMKSLHMQSFDK*L FRPYNVSGTDM*NFFN*KRAVIWQKVSM*YFSC LFS*TQTSGKNLPSVVIGEFKRTVSLQPISVCP YQNYK*GGQQAPRK*GLSMSCSKLYIQ*LTLCLA HNTH*VNIC*VK*NQETSKN*

Frame 1

IKTFHPSV*KDKRKKVKQLQEMALGVPISVYLL FNAMTALTEEAAVTVTPPITAQQADNIEGPIAL KFSHLCLEDHNSYCINGACAFHHELEKAICRCFT GYTGERCEHLTLTSYAVDSYEKYIAIGIGVGLL LSGFLVIFYCYIRKRYEKDKI*SHFICNRLTNS YSGPIMCQALTCKIFLIKKEL*SGKKFLCNIFHA FSHKPRRVVKICLQL**ESSNVQSPFNLSLSAH IKIINEEDSRPQESRD*VCLVQNCIFSDLHYA* HTTHTE*IFVE*NKIKKQVKT

Frame 2

*KPSTRQSRRIRERKLSNYRKWLWEFQYQSIFY STQ*QH*PKRQP*L*HLQSQPSKLTT*KDP*P* SSHTFAWKIITVTASTVLVHSTMS*RKPSAGVL LVILEKGVST*L*LHMLWILMKNTLQLGLVLDYY *VVFLLFFTAI*ERGMKKTKYEVTSYAIV*QIV IQAL*CVRH*HVKFF*LKKSCNLAKSFYVIFFM PFLINPDEW*KFAFSCNRRVQTYSLPSTYLCLPI SKL*MRRTAGPKKVGTKYVLFKIVYSVTYTMPS TQHTLSKYLLSEIKSRNK*KL

Sequence EPGN.eAug05

AATAAAAACCTTCCACCCGTCAGTCTAGAAGGATAAGAGAAAGAAAGTTAAGCAACTACAGGAAATGGCTTTGGGAGTTCCAATATCAGTCTATCTTTTA TTCAACGCAATGACAGCACTGACCGAAGAGGCAGCCGTGACTGTAACACCTCCAATCACAGCCCAGCAAGGTAACTGGACAGTTAACAAAACAGAAGCTG ACAACATAGAAGGACCCATAGCCTTGAAGTTCTCACACCTTTGCCTGGAAGATCATAACAGTTACTGCATCAACGGTGCTTGTGCATTCCACCATGAGCT AGAGAAAGCCATCTGCAGGTGTCTAAAATTGAAATCGCCTTACAATGTCTGTTCTGGAGAAAGACGACCACTGTGAGGCCTTTGTGAAGAATTTTCATCA AGGCATCTGTAGAGATCAGTGAGCCCAAAATTAAAGTTTTCAGATGAAACAACAAAACTTGTCAAGCTGACTAGACTCGAAAATAATGAAAGTTGGGATC ACAATGAAATGAGAAGATAAAATTCAGCGTTGGCCTTTAGACTTTGCCATCCTTAAGGAGTGATGGAAGCCAAGTGAACAAGCCTCAGTGACACAAGTCA AATTCATAGTTTCACTCTGGGTTTTTTGTTGTTGTGTGGTTATTATTCTCACTACAGAAAGACTGAGTTTCATGCTCCTGGCTATGTCAGATGTGAATTT TCATGGGAATAATAATCAACCTTGCAGCAAGCCAAAGCAATGCCTCGCTTGGGTTCTTCATGTTCTTACTACCCAGCGTTTTTTACCACCTAGATGGGCC TCTCTAAGTCTATTTGCTCAATGAACCTTATCCCAAACTTGTTGTTTTCATGGTGTCTGAAAGAATGTGATGTCAGCTTTTAGAATGAAATCAGTGTTAA GGTACCTCCAGTGAACCAAACATGTGTCTTAAATCTAAGCCACTGAAAACAGAAGGGAATGTCCAAGGCAAATACAAATCATACACAGCTTGTAACAACA TACAGCCTTTCTATGTGAAATAATGGAATAAACTAAAGAGTTTCTGAAGACTGAAGCTATCTGGATATCAAGTCTGGAGTAGGCAAAGCATTTCCATTTC TACATGGATTATAAAACTTTGTGTTGGACTCATTGGTCCCTAATGCTTTTGTTCATCACTTCTTCCAGTTATCAGTGGAATTACCTGGTGACCATTCATT TGGACCGAAATCCTGGAAGTCTCCTACTGAATAAAAGTCTACAATTGGCCCTAAAATAGAAACTGAAAAACAGGACATAGAATTTTTTCACCAGACCACA GCATGTGGAAACTTTCTTTATCATTTTTGAACACTTGTTAACAGATTTGCACATAGAGGGAGAGAAAAAAAATGGAGTAACAGTCAAAATAATAATAATC AGTATCCAGGCCAGGCGTGGTGGTTCACGCCTGTAATCCTAGCACTTTGGGAGGCCGAGGCGGATGGAGCACCTGAGGTCAGGAGTTTGAGACCAGCCTG GCCAACATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCTGGGCGTGGTGGTGCATGCCTGTAATCCCAGCTACTCCAGAGGCTGACACAGGA CAATCACTTGAACCCAGGAGGCAGAGGTTGTAGTGAGCCGAGATGGCAACCCTGCACTCCAACCTGGGTGACAAGAGCGAAACTCCATCTCAAAAAAAAA AAAAAAAA

EPGN.F3 (forward primer)
EPGN.R4 (reverse primer)
Alternative donor

Frame 0

NKNLPPVSLEG*EKES*ATTGNGFGSSNISLSF IQRNDSTDRRGSRDCNTSNHSPAR*LDS*QNRS* QHRRTHSLEVLTPLPGRS*QLLHQRCLCIPP*A RESHLQVSKIEIALQCLFWRKTTTVRPL*RIFI KASVEISEPKIKVFR*NNKTCQAD*TRK**KLGS Q*NEKIKFSVGL*TLPSLRSDGSQVNKPQ*HKS NS*FHSGFFVVVWLLFSLQKD*VSCSWLCQM*I FMGIIINLAASQSNASLGFFMFLLPSVFYHLDGP L*VYLLNEPYPKLVVFMVSERM*CQLLE*NQC* GTSSEPNMCLKSKPLKTEGNVQGKYKSYTACNN IQPFYVK*WNKLKSF*RLKLSGYQVWSRQSISIS TWIIKLCVGLIGP*CFCSSLLPVISGITW*PFI WTEILEVSY*IKVYNWP*NRN*KTGHRIFSPDH SMWKLSLSFLNTC*QICT*RERKKME*QSK***S VSRPGVVVHACNPSTLGGRGGWST*GQEFETSL ANMVKPCLY*KYKN*LGVVVHACNPSYSRG*HR TIT*TQEAEVVVSRDGNPALQPG*QERNSISKKK KK

Frame 1

IKTFHPSV*KDKRKKVKQLQEMALGVPISVYLL FNAMTALTEEAAVTVTPPITAQQGNWTVNKTEA DNIEGPIALKFSHLCLEDHNSYCINGACAFHHEL EKAICRCLKLKSPYNVCSGERRPL*GLCEEFSS RHL*RSVSPKLKFSDETTKLVKLTRLENNESWD HNEMRR*NSALAFRLCHP*GVMEAK*TSLSDTSQ IHSFTLGFLLLCGYYSHYRKTEFHAPGYVRCEF SWE**STLQQAKAMPRLGSSCSYYPAFFTT*MG LSKSICSMNLIPNLLFSWCLKECDVSF*NEISVK VPPVNQTCVLNLSH*KQKGMSKANTNHTQLVTT YSLSM*NNGIN*RVSED*SYLDIKSGVGKAFPF LHGL*NFVLDSLVPNAFVHHFFQLSVELPGDHSF GPKSWKSPTE*KSTIGPKIETEKQDIEFFHQTT ACGNFLYHF*TLVNRFAHRGREKKWSNSQNNNN QYPGQAWWFTPVILALWEAEADGAPEVRSLRPAW PTW*NPVSTKNTKISWAWWCMPVIPATPEADTG QSLEPRRQRL**AEMATLHSNLGDKSETPSQKK KKK

Frame 2

*KPSTRQSRRIRERKLSNYRKWLWEFQYQSIFY STQ*QH*PKRQP*L*HLQSQPSKVTGQLTKQKL TT*KDP*P*SSHTFAWKIITVTASTVLVHSTMS *RKPSAGV*N*NRLTMSVLEKDDHCEAFVKNFHQ GICRDQ*AQN*SFQMKQQNLSS*LDSKIMKVGI TMK*EDKIQRWPLDFAILKE*WKPSEQASVTQV KFIVSLWVFCCCVVIILTTERLSFMLLAMSDVNF HGNNNQPCSKPKQCLAWVLHVLTTQRFLPPRWA SLSLFAQ*TLSQTCCFHGV*KNVMSAFRMKSVL RYLQ*TKHVS*I*ATENRRECPRQIQIIHSL*QH TAFLCEIME*TKEFLKTEAIWISSLE*AKHFHF YMDYKTLCWTHWSLMLLFITSSSYQWNYLVTIH LDRNPGSLLLNKSLQLALK*KLKNRT*NFFTRPQ HVETFFIIFEHLLTDLHIEGEKKNGVTVKIIII SIQARRGGSRL*S*HFGRPRRMEHLRSGV*DQP GQHGETLSLLKIQKLAGRGGACL*SQLLQRLTQD NHLNPGGRGCSEPRWQPCTPTWVTRAKLHLKKK KK

Sequence EPGN.dAug05

AATAAAAACCTTCCACCCGTCAGTCTAGAAGGATAAGAGAAAGAAAGTTAAGCAACTACAGGAAATGGCTTTGGGAGTTCCAATATCAGTCTATCTTTTA TTCAACGCAATGACAGCACTGACCGAAGAGGCAGCCGTGACTGTAACACCTCCAATCACAGCCCAGCAAGGTAACTGGACAGTTAACAAAACAGAAGCTG ACAACATAGAAGGACCCATAGCCTTGAAGTTCTCACACCTTTGCCTGGAAGATCATAACAGTTACTGCATCAACGGTGCTTGTGCATTCCACCATGAGCT AGAGAAAGCCATCTGCAGGTGTTTTACTGGTTATACTGGAGAAAGGTGTCTAAAATTGAAATCGCCTTACAATGTCTGTTCTGGAGAAAGACGACCACTG TGAGGCCTTTGTGAAGAATTTTCATCAAGGCATCTGTAGAGATCAGTGAGCCCAAAATTAAAGTTTTCAGATGAAACAACAAAACTTGTCAAGCTGACTA GACTCGAAAATAATGAAAGTTGGGATCACAATGAAATGAGAAGATAAAATTCAGCGTTGGCCTTTAGACTTTGCCATCCTTAAGGAGTGATGGAAGCCAA GTGAACAAGCCTCAGTGACACAAGTCAAATTCATAGTTTCACTCTGGGTTTTTTGTTGTTGTGTGGTTATTATTCTCACTACAGAAAGACTGAGTTTCAT GCTCCTGGCTATGTCAGATGTGAATTTTCATGGGAATAATAATCAACCTTGCAGCAAGCCAAAGCAATGCCTCGCTTGGGTTCTTCATGTTCTTACTACC CAGCGTTTTTTACCACCTAGATGGGCCTCTCTAAGTCTATTTGCTCAATGAACCTTATCCCAAACTTGTTGTTTTCATGGTGTCTGAAAGAATGTGATGT CAGCTTTTAGAATGAAATCAGTGTTAAGGTACCTCCAGTGAACCAAACATGTGTCTTAAATCTAAGCCACTGAAAACAGAAGGGAATGTCCAAGGCAAAT ACAAATCATACACAGCTTGTAACAACATACAGCCTTTCTATGTGAAATAATGGAATAAACTAAAGAGTTTCTGAAGACTGAAGCTATCTGGATATCAAGT CTGGAGTAGGCAAAGCATTTCCATTTCTACATGGATTATAAAACTTTGTGTTGGACTCATTGGTCCCTAATGCTTTTGTTCATCACTTCTTCCAGTTATC AGTGGAATTACCTGGTGACCATTCATTTGGACCGAAATCCTGGAAGTCTCCTACTGAATAAAAGTCTACAATTGGCCCTAAAATAGAAACTGAAAAACAG GACATAGAATTTTTTCACCAGACCACAGCATGTGGAAACTTTCTTTATCATTTTTGAACACTTGTTAACAGATTTGCACATAGAGGGAGAGAAAAAAAAT GGAGTAACAGTCAAAATAATAATAATCAGTATCCAGGCCAGGCGTGGTGGTTCACGCCTGTAATCCTAGCACTTTGGGAGGCCGAGGCGGATGGAGCACC TGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCTGGGCGTGGTGGTGCATGCCTGTAATC CCAGCTACTCCAGAGGCTGACACAGGACAATCACTTGAACCCAGGAGGCAGAGGTTGTAGTGAGCCGAGATGGCAACCCTGCACTCCAACCTGGGTGACA AGAGCGAAACTCCATCTCAAAAAAAAAAAAAAAAA

EPGN.F3 (forward primer)
EPGN.R4 (reverse primer)
Alternative donor

Frame 0

NKNLPPVSLEG*EKES*ATTGNGFGSSNISLSF IQRNDSTDRRGSRDCNTSNHSPAR*LDS*QNRS* QHRRTHSLEVLTPLPGRS*QLLHQRCLCIPP*A RESHLQVFYWLYWRKVSKIEIALQCLFWRKTTT VRPL*RIFIKASVEISEPKIKVFR*NNKTCQAD* TRK**KLGSQ*NEKIKFSVGL*TLPSLRSDGSQ VNKPQ*HKSNS*FHSGFFVVVWLLFSLQKD*VS CSWLCQM*IFMGIIINLAASQSNASLGFFMFLLP SVFYHLDGPL*VYLLNEPYPKLVVFMVSERM*C QLLE*NQC*GTSSEPNMCLKSKPLKTEGNVQGK YKSYTACNNIQPFYVK*WNKLKSF*RLKLSGYQV WSRQSISISTWIIKLCVGLIGP*CFCSSLLPVI SGITW*PFIWTEILEVSY*IKVYNWP*NRN*KT GHRIFSPDHSMWKLSLSFLNTC*QICT*RERKKM E*QSK***SVSRPGVVVHACNPSTLGGRGGWST *GQEFETSLANMVKPCLY*KYKN*LGVVVHACN PSYSRG*HRTIT*TQEAEVVVSRDGNPALQPG*Q ERNSISKKKKK

Frame 1

IKTFHPSV*KDKRKKVKQLQEMALGVPISVYLL FNAMTALTEEAAVTVTPPITAQQGNWTVNKTEA DNIEGPIALKFSHLCLEDHNSYCINGACAFHHEL EKAICRCFTGYTGERCLKLKSPYNVCSGERRPL *GLCEEFSSRHL*RSVSPKLKFSDETTKLVKLT RLENNESWDHNEMRR*NSALAFRLCHP*GVMEAK *TSLSDTSQIHSFTLGFLLLCGYYSHYRKTEFH APGYVRCEFSWE**STLQQAKAMPRLGSSCSYY PAFFTT*MGLSKSICSMNLIPNLLFSWCLKECDV SF*NEISVKVPPVNQTCVLNLSH*KQKGMSKAN TNHTQLVTTYSLSM*NNGIN*RVSED*SYLDIK SGVGKAFPFLHGL*NFVLDSLVPNAFVHHFFQLS VELPGDHSFGPKSWKSPTE*KSTIGPKIETEKQ DIEFFHQTTACGNFLYHF*TLVNRFAHRGREKK WSNSQNNNNQYPGQAWWFTPVILALWEAEADGAP EVRSLRPAWPTW*NPVSTKNTKISWAWWCMPVI PATPEADTGQSLEPRRQRL**AEMATLHSNLGD KSETPSQKKKKK

Frame 2

*KPSTRQSRRIRERKLSNYRKWLWEFQYQSIFY STQ*QH*PKRQP*L*HLQSQPSKVTGQLTKQKL TT*KDP*P*SSHTFAWKIITVTASTVLVHSTMS *RKPSAGVLLVILEKGV*N*NRLTMSVLEKDDHC EAFVKNFHQGICRDQ*AQN*SFQMKQQNLSS*L DSKIMKVGITMK*EDKIQRWPLDFAILKE*WKP SEQASVTQVKFIVSLWVFCCCVVIILTTERLSFM LLAMSDVNFHGNNNQPCSKPKQCLAWVLHVLTT QRFLPPRWASLSLFAQ*TLSQTCCFHGV*KNVM SAFRMKSVLRYLQ*TKHVS*I*ATENRRECPRQI QIIHSL*QHTAFLCEIME*TKEFLKTEAIWISS LE*AKHFHFYMDYKTLCWTHWSLMLLFITSSSY QWNYLVTIHLDRNPGSLLLNKSLQLALK*KLKNR T*NFFTRPQHVETFFIIFEHLLTDLHIEGEKKN GVTVKIIIISIQARRGGSRL*S*HFGRPRRMEH LRSGV*DQPGQHGETLSLLKIQKLAGRGGACL*S QLLQRLTQDNHLNPGGRGCSEPRWQPCTPTWVT RAKLHLKKKKK

Sequence EPGN.aAug05

AATAAAAACCTTCCACCCGTCAGTCTAGAAGGATAAGAGAAAGAAAGTTAAGCAACTACAGGAAATGGCTTTGGGAGTTCCAATATCAGTCTATCTTTTA TTCAACGCAATGACAGCACTGACCGAAGAGGCAGCCGTGACTGTAACACCTCCAATCACAGCCCAGCAAGGTAACTGGACAGTTAACAAAACAGAAGCTG ACAACATAGAAGGACCCATAGCCTTGAAGTTCTCACACCTTTGCCTGGAAGATCATAACAGTTACTGCATCAACGGTGCTTGTGCATTCCACCATGAGCT AGAGAAAGCCATCTGCAGGTGTTTTACTGGTTATACTGGAGAAAGGTGTGAGCACTTGACTTTAACTTCATATGCTGTGGATTCTTATGAAAAATACATT GCAATTGGGATTGGTGTTGGATTACTATTAAGTGGTTTTCTTGTTATTTTTTACTGCTATATAAGAAAGAGGTGTCTAAAATTGAAATCGCCTTACAATG TCTGTTCTGGAGAAAGACGACCACTGTGAGGCCTTTGTGAAGAATTTTCATCAAGGCATCTGTAGAGATCAGTGAGCCCAAAATTAAAGTTTTCAGATGA AACAACAAAACTTGTCAAGCTGACTAGACTCGAAAATAATGAAAGTTGGGATCACAATGAAATGAGAAGATAAAATTCAGCGTTGGCCTTTAGACTTTGC CATCCTTAAGGAGTGATGGAAGCCAAGTGAACAAGCCTCAGTGACACAAGTCAAATTCATAGTTTCACTCTGGGTTTTTTGTTGTTGTGTGGTTATTATT CTCACTACAGAAAGACTGAGTTTCATGCTCCTGGCTATGTCAGATGTGAATTTTCATGGGAATAATAATCAACCTTGCAGCAAGCCAAAGCAATGCCTCG CTTGGGTTCTTCATGTTCTTACTACCCAGCGTTTTTTACCACCTAGATGGGCCTCTCTAAGTCTATTTGCTCAATGAACCTTATCCCAAACTTGTTGTTT TCATGGTGTCTGAAAGAATGTGATGTCAGCTTTTAGAATGAAATCAGTGTTAAGGTACCTCCAGTGAACCAAACATGTGTCTTAAATCTAAGCCACTGAA AACAGAAGGGAATGTCCAAGGCAAATACAAATCATACACAGCTTGTAACAACATACAGCCTTTCTATGTGAAATAATGGAATAAACTAAAGAGTTTCTGA AGACTGAAGCTATCTGGATATCAAGTCTGGAGTAGGCAAAGCATTTCCATTTCTACATGGATTATAAAACTTTGTGTTGGACTCATTGGTCCCTAATGCT TTTGTTCATCACTTCTTCCAGTTATCAGTGGAATTACCTGGTGACCATTCATTTGGACCGAAATCCTGGAAGTCTCCTACTGAATAAAAGTCTACAATTG GCCCTAAAATAGAAACTGAAAAACAGGACATAGAATTTTTTCACCAGACCACAGCATGTGGAAACTTTCTTTATCATTTTTGAACACTTGTTAACAGATT TGCACATAGAGGGAGAGAAAAAAAATGGAGTAACAGTCAAAATAATAATAATCAGTATCCAGGCCAGGCGTGGTGGTTCACGCCTGTAATCCTAGCACTT TGGGAGGCCGAGGCGGATGGAGCACCTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCT GGGCGTGGTGGTGCATGCCTGTAATCCCAGCTACTCCAGAGGCTGACACAGGACAATCACTTGAACCCAGGAGGCAGAGGTTGTAGTGAGCCGAGATGGC AACCCTGCACTCCAACCTGGGTGACAAGAGCGAAACTCCATCTCAAAAAAAAAAAAAAAAA

EPGN.F3 (forward primer)
EPGN.R4 (reverse primer)
Alternative donor

Frame 0

NKNLPPVSLEG*EKES*ATTGNGFGSSNISLSF IQRNDSTDRRGSRDCNTSNHSPAR*LDS*QNRS* QHRRTHSLEVLTPLPGRS*QLLHQRCLCIPP*A RESHLQVFYWLYWRKV*ALDFNFICCGFL*KIH CNWDWCWITIKWFSCYFLLLYKKEVSKIEIALQC LFWRKTTTVRPL*RIFIKASVEISEPKIKVFR* NNKTCQAD*TRK**KLGSQ*NEKIKFSVGL*TL PSLRSDGSQVNKPQ*HKSNS*FHSGFFVVVWLLF SLQKD*VSCSWLCQM*IFMGIIINLAASQSNAS LGFFMFLLPSVFYHLDGPL*VYLLNEPYPKLVV FMVSERM*CQLLE*NQC*GTSSEPNMCLKSKPLK TEGNVQGKYKSYTACNNIQPFYVK*WNKLKSF* RLKLSGYQVWSRQSISISTWIIKLCVGLIGP*C FCSSLLPVISGITW*PFIWTEILEVSY*IKVYNW P*NRN*KTGHRIFSPDHSMWKLSLSFLNTC*QI CT*RERKKME*QSK***SVSRPGVVVHACNPST LGGRGGWST*GQEFETSLANMVKPCLY*KYKN*L GVVVHACNPSYSRG*HRTIT*TQEAEVVVSRDG NPALQPG*QERNSISKKKKK

Frame 1

IKTFHPSV*KDKRKKVKQLQEMALGVPISVYLL FNAMTALTEEAAVTVTPPITAQQGNWTVNKTEA DNIEGPIALKFSHLCLEDHNSYCINGACAFHHEL EKAICRCFTGYTGERCEHLTLTSYAVDSYEKYI AIGIGVGLLLSGFLVIFYCYIRKRCLKLKSPYN VCSGERRPL*GLCEEFSSRHL*RSVSPKLKFSDE TTKLVKLTRLENNESWDHNEMRR*NSALAFRLC HP*GVMEAK*TSLSDTSQIHSFTLGFLLLCGYY SHYRKTEFHAPGYVRCEFSWE**STLQQAKAMPR LGSSCSYYPAFFTT*MGLSKSICSMNLIPNLLF SWCLKECDVSF*NEISVKVPPVNQTCVLNLSH* KQKGMSKANTNHTQLVTTYSLSM*NNGIN*RVSE D*SYLDIKSGVGKAFPFLHGL*NFVLDSLVPNA FVHHFFQLSVELPGDHSFGPKSWKSPTE*KSTI GPKIETEKQDIEFFHQTTACGNFLYHF*TLVNRF AHRGREKKWSNSQNNNNQYPGQAWWFTPVILAL WEAEADGAPEVRSLRPAWPTW*NPVSTKNTKIS WAWWCMPVIPATPEADTGQSLEPRRQRL**AEMA TLHSNLGDKSETPSQKKKKK

Frame 2

*KPSTRQSRRIRERKLSNYRKWLWEFQYQSIFY STQ*QH*PKRQP*L*HLQSQPSKVTGQLTKQKL TT*KDP*P*SSHTFAWKIITVTASTVLVHSTMS *RKPSAGVLLVILEKGVST*L*LHMLWILMKNTL QLGLVLDYY*VVFLLFFTAI*ERGV*N*NRLTM SVLEKDDHCEAFVKNFHQGICRDQ*AQN*SFQM KQQNLSS*LDSKIMKVGITMK*EDKIQRWPLDFA ILKE*WKPSEQASVTQVKFIVSLWVFCCCVVII LTTERLSFMLLAMSDVNFHGNNNQPCSKPKQCL AWVLHVLTTQRFLPPRWASLSLFAQ*TLSQTCCF HGV*KNVMSAFRMKSVLRYLQ*TKHVS*I*ATE NRRECPRQIQIIHSL*QHTAFLCEIME*TKEFL KTEAIWISSLE*AKHFHFYMDYKTLCWTHWSLML LFITSSSYQWNYLVTIHLDRNPGSLLLNKSLQL ALK*KLKNRT*NFFTRPQHVETFFIIFEHLLTD LHIEGEKKNGVTVKIIIISIQARRGGSRL*S*HF GRPRRMEHLRSGV*DQPGQHGETLSLLKIQKLA GRGGACL*SQLLQRLTQDNHLNPGGRGCSEPRW QPCTPTWVTRAKLHLKKKKK