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Sequences

Sequence PPP1R14C.dAug05

CAGCCGGGCGGCTGGGCGCGCGCGGCGCAGAGCAGGTGCCGGGGAGCCCTTCGCATGCGGCTGCCGGGCCGGAGGTGGTAGCGGCGCCGGGCGCGCTCCG CCCGCCCCTCCTCCGGGCCGCACTGAGGCTCGGGCGCGCGGGGACATGTCGGTGGCGACGGGCAGCAGCGAGACGGCCGGCGGGGCCAGCGGCGGCGGCG CACGGGTTTTCTTCCAAAGCCCCCGGGGTGGCGCCGGTGGCAGCCCCGGCTCCAGCAGCGGCTCAGGCTCCTCCCGGGAGGACTCGGCGCCCGTGGCCAC GGCGGCCGCTGCAGGGCAGGTTCAGCAGCAACAGCAGCGGCGACACCAGCAGGGAAAAGTGACAGTGAAATACGATCGTAAGGAGCTTCGGAAGCGGCTG GTGCTGGAGGAATGGATCGTGGAGCAGCTGGGTCAGCTCTACGGCTGCGAGGAATTTATCAAAGAGCTGCTTTCTCGGATAAGAGGCATGAGGAAACTGA GCCCTCCGCAGAAGAAGAGTGTATGATTCTGGAACAGGGTGAAACTCTCCCAGAGACGAAGAAAGAGTCCTGGGATTTGTACTTCATGAAGACTTTTGTG AAAGAATAGGTGTCCTTATGAACAACGTTTTTGTTTTTTTTTTTTTCTTTTTTGGTGTGAAGGTGGGGGGGTCTATTAGACATTTATTCAAGAGCGTTCT TTTTTTGGTTTTAAAGGTTTTTGTTAATGTAATATTTTAATAGCAAAGATATCATGACTCTAGCCACAGCCTAACCAAGGATTATCAAAGGAGGTGGACA CTCAAGGAAGGGCCACGCCAGGCTGCGTTTCCTGCAAGGACTCAGATGTTCAGTACCTTATGATACAGGGAAGATAGTTTTCTTACAAGTAGTTTGGTAA TATTTTTTTTCTTAAGTTGTACATTTGACTCAGCTGTCAAATTTCTCACACTTGTATATATCTACACACAACTAAGTTAAAATGTTGATGTGAGTTTTAT TTCACATGGATGGAATAAACTTGTGGTTGTCCTTTAACTGGAGGTCCCAGCACATGTGTTTTCAAGAGGCCACTAGGCATTCTTCACTGAGTGCTGCTGA CTTCAACGTTCACTTTATGCACCAAAGTGAAAGAATTCAGTGTATCCGTTATTTTAATGCACTACACCACAGAAATGTTAAGTTGGTCAAGGGCTTAATT TATGGAATTTCTATTATTTCATTGGTTGGGATGCTTTGCCAGGTCATGTGCTTATTGTCTCATTTTGTAGTCTTTTAAAGTTGTATGAACCCTTAATTTG AAGAACTAACTTGATTTCTAGAGAAATATCCACACTATCTCAGTGGTATTTTGCATTGGAAAAAGGAAGCACTGTGTAGCAGTGAATTGTCTGCTTTCCA CCGAGTACTGTGTTTATTCTCTCTCCAGGAAAGCAGATCAAAAGAAAGTTAGCAGATCGAGTGTCTTCTTCCTTAGAAATAGGTTCTGGTAGCTTCTGTG CCTGGGTAGTATCAGACCAGTGGGAGTAAACCGAGTGTTAAGTGTCAAGGTGAGAAAGCCTCACATTCTCTCAAGACAGTTGCTCTAGGAGCTGAGTTGC TGGTTTGGAAGTGTGGAGATTGCATTTCTGGCTTCTCTCAATGGCTTGTGTTGAGGACTCTGGGTGCTCCTGGCCCTAATTGTGCACCCTGATCCCCGTG CTTGGAGCTAGGCCTGGTGGCGTGCTCTAGCCCTCTCAGTTACCAGCTCTTTGGAGAAGGATCAAAATTCAGATGGAATGTGGGATGGGTAATAGGTGAG AGTAGAAACCCTTCCCTCCAGGAGGCCCCCGCTGACTCCCACAGAAACCCACCTACCATAAGATGTCTTCAGGTGGCCTTGTCCAAGGATGGGGGTCAGG CATTTTATATCAAGGGTGCTCTGAACATATTTTATTTTTTAAAAAAACTATGTTTGTGAATTTTGCGTATACTGGCAAGCTTTTGAAAATGTATTTAATT TTGTATTGTTTACCAATGATTTATTTACAAGATATTTACTCAAATAAATGGAGCTGCTTACAAGCCTGTTGACATGTGTGGCTTGCACAACACGTTAA

PPP1R14C.F2 (forward primer)
PPP1R14C.R2 (reverse primer)
Multiple exon skipping

Frame 0

QPGGWARAAQSRCRGALRMRLPGRRW*RRRARS ARPSSGPH*GSGARGHVGGDGQQRDGRRGQRRRR TGFLPKPPGWRRWQPRLQQRLRLLPGGLGARGH GGRCRAGSAATAAATPAGKSDSEIRS*GASEAA GAGGMDRGAAGSALRLRGIYQRAAFSDKRHEETE PSAEEECMILEQGETLPETKKESWDLYFMKTFV KE*VSL*TTFLFFFFSFLV*RWGGLLDIYSRAF FFWF*RFLLM*YFNSKDIMTLATA*PRIIKGGGH SRKGHARLRFLQGLRCSVPYDTGKIVFLQVVW* YFFS*VVHLTQLSNFSHLYISTHN*VKMLM*VL FHMDGINLWLSFNWRSQHMCFQEATRHSSLSAAD FNVHFMHQSERIQCIRYFNALHHRNVKLVKGLI YGISIISLVGMLCQVMCLLSHFVVF*SCMNP*F EELT*FLEKYPHYLSGILHWKKEALCSSELSAFH RVLCLFSLQESRSKES*QIECLLP*K*VLVASV PG*YQTSGSKPSVKCQGEKASHSLKTVALGAEL LVWKCGDCISGFSQWLVLRTLGAPGPNCAP*SPC LELGLVACSSPLSYQLFGEGSKFRWNVGWVIGE SRNPSLQEAPADSHRNPPTIRCLQVALSKDGGQ AFYIKGALNIFYFLKKLCL*ILRILASF*KCI*F CIVYQ*FIYKIFTQINGAAYKPVDMCGLHNTL

Frame 1

SRAAGRARRRAGAGEPFACGCRAGGGSGAGRAP PAPPPGRTEARARGDMSVATGSSETAGGASGGG ARVFFQSPRGGAGGSPGSSSGSGSSREDSAPVAT AAAAGQVQQQQQRRHQQGKVTVKYDRKELRKRL VLEEWIVEQLGQLYGCEEFIKELLSRIRGMRKL SPPQKKSV*FWNRVKLSQRRRKSPGICTS*RLL* KNRCPYEQRFCFFFFLFWCEGGGVY*TFIQERS FFGFKGFC*CNILIAKIS*L*PQPNQGLSKEVD TQGRATPGCVSCKDSDVQYLMIQGR*FSYK*FGN IFFLKLYI*LSCQISHTCIYLHTTKLKC*CEFY FTWME*TCGCPLTGGPSTCVFKRPLGILH*VLL TSTFTLCTKVKEFSVSVILMHYTTEMLSWSRA*F MEFLLFHWLGCFARSCAYCLIL*SFKVV*TLNL KN*LDF*RNIHTISVVFCIGKRKHCVAVNCLLS TEYCVYSLSRKADQKKVSRSSVFFLRNRFW*LLC LGSIRPVGVNRVLSVKVRKPHILSRQLL*ELSC WFGSVEIAFLASLNGLC*GLWVLLALIVHPDPR AWS*AWWRALALSVTSSLEKDQNSDGMWDG**VR VETLPSRRPPLTPTETHLP*DVFRWPCPRMGVR HFISRVL*TYFIF*KNYVCEFCVYWQAFENVFN FVLFTNDLFTRYLLK*MELLTSLLTCVACTTR*

Frame 2

AGRLGARGAEQVPGSPSHAAAGPEVVAAPGALR PPLLRAALRLGRAGTCRWRRAAARRPAGPAAAA HGFSSKAPGVAPVAAPAPAAAQAPPGRTRRPWP RRPLQGRFSSNSSGDTSREK*Q*NTIVRSFGSGW CWRNGSWSSWVSSTAARNLSKSCFLG*EA*GN* ALRRRRVYDSGTG*NSPRDEERVLGFVLHEDFC ERIGVLMNNVFVFFFFFFGVKVGGSIRHLFKSVL FLVLKVFVNVIF**QRYHDSSHSLTKDYQRRWT LKEGPRQAAFPARTQMFSTL*YREDSFLTSSLV IFFFLSCTFDSAVKFLTLVYIYTQLS*NVDVSFI SHGWNKLVVVL*LEVPAHVFSRGH*AFFTECC* LQRSLYAPK*KNSVYPLF*CTTPQKC*VGQGLN LWNFYYFIGWDALPGHVLIVSFCSLLKLYEPLI* RTNLISREISTLSQWYFALEKGSTV*Q*IVCFP PSTVFILSPGKQIKRKLADRVSSSLEIGSGSFC AWVVSDQWE*TEC*VSR*ESLTFSQDSCSRS*VA GLEVWRLHFWLLSMACVEDSGCSWP*LCTLIPV LGARPGGVL*PSQLPALWRRIKIQMECGMGNR* E*KPFPPGGPR*LPQKPTYHKMSSGGLVQGWGSG ILYQGCSEHILFFKKTMFVNFAYTGKLLKMYLI LYCLPMIYLQDIYSNKWSCLQAC*HVWLAQHV

Sequence PPP1R14C.aAug05

CAGCCGGGCGGCTGGGCGCGCGCGGCGCAGAGCAGGTGCCGGGGAGCCCTTCGCATGCGGCTGCCGGGCCGGAGGTGGTAGCGGCGCCGGGCGCGCTCCG CCCGCCCCTCCTCCGGGCCGCACTGAGGCTCGGGCGCGCGGGGACATGTCGGTGGCGACGGGCAGCAGCGAGACGGCCGGCGGGGCCAGCGGCGGCGGCG CACGGGTTTTCTTCCAAAGCCCCCGGGGTGGCGCCGGTGGCAGCCCCGGCTCCAGCAGCGGCTCAGGCTCCTCCCGGGAGGACTCGGCGCCCGTGGCCAC GGCGGCCGCTGCAGGGCAGGTTCAGCAGCAACAGCAGCGGCGACACCAGCAGGGAAAAGTGACAGTGAAATACGATCGTAAGGAGCTTCGGAAGCGGCTG GTGCTGGAGGAATGGATCGTGGAGCAGCTGGGTCAGCTCTACGGCTGCGAGGAAGAAGAAATGCCAGAGGTAGAAATTGACATTGATGATCTTCTTGATG CAGACAGTGATGAAGAGAGAGCTTCAAAATTACAGGAAGCTCTTGTAGACTGCTACAAACCAACAGAGACGGAGTCTCGCTCTGTTGCGCAGGTTGGAGT GCAGTGGTGTGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGTTCAACCAATTCTCCCGCCTCAGCCTCCTGAGTAGCTGGGATTACAGACGTGCGTCA CCACGCCCGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTATTCACCCGCCTCGG CCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCCGGCCTATCGGAATTTATCAAAGAGCTGCTTTCTCGGATAAGAGGCATGAGGAAACTGA GCCCTCCGCAGAAGAAGAGTGTATGATTCTGGAACAGGGTGAAACTCTCCCAGAGACGAAGAAAGAGTCCTGGGATTTGTACTTCATGAAGACTTTTGTG AAAGAATAGGTGTCCTTATGAACAACGTTTTTGTTTTTTTTTTTTTCTTTTTTGGTGTGAAGGTGGGGGGGTCTATTAGACATTTATTCAAGAGCGTTCT TTTTTTGGTTTTAAAGGTTTTTGTTAATGTAATATTTTAATAGCAAAGATATCATGACTCTAGCCACAGCCTAACCAAGGATTATCAAAGGAGGTGGACA CTCAAGGAAGGGCCACGCCAGGCTGCGTTTCCTGCAAGGACTCAGATGTTCAGTACCTTATGATACAGGGAAGATAGTTTTCTTACAAGTAGTTTGGTAA TATTTTTTTTCTTAAGTTGTACATTTGACTCAGCTGTCAAATTTCTCACACTTGTATATATCTACACACAACTAAGTTAAAATGTTGATGTGAGTTTTAT TTCACATGGATGGAATAAACTTGTGGTTGTCCTTTAACTGGAGGTCCCAGCACATGTGTTTTCAAGAGGCCACTAGGCATTCTTCACTGAGTGCTGCTGA CTTCAACGTTCACTTTATGCACCAAAGTGAAAGAATTCAGTGTATCCGTTATTTTAATGCACTACACCACAGAAATGTTAAGTTGGTCAAGGGCTTAATT TATGGAATTTCTATTATTTCATTGGTTGGGATGCTTTGCCAGGTCATGTGCTTATTGTCTCATTTTGTAGTCTTTTAAAGTTGTATGAACCCTTAATTTG AAGAACTAACTTGATTTCTAGAGAAATATCCACACTATCTCAGTGGTATTTTGCATTGGAAAAAGGAAGCACTGTGTAGCAGTGAATTGTCTGCTTTCCA CCGAGTACTGTGTTTATTCTCTCTCCAGGAAAGCAGATCAAAAGAAAGTTAGCAGATCGAGTGTCTTCTTCCTTAGAAATAGGTTCTGGTAGCTTCTGTG CCTGGGTAGTATCAGACCAGTGGGAGTAAACCGAGTGTTAAGTGTCAAGGTGAGAAAGCCTCACATTCTCTCAAGACAGTTGCTCTAGGAGCTGAGTTGC TGGTTTGGAAGTGTGGAGATTGCATTTCTGGCTTCTCTCAATGGCTTGTGTTGAGGACTCTGGGTGCTCCTGGCCCTAATTGTGCACCCTGATCCCCGTG CTTGGAGCTAGGCCTGGTGGCGTGCTCTAGCCCTCTCAGTTACCAGCTCTTTGGAGAAGGATCAAAATTCAGATGGAATGTGGGATGGGTAATAGGTGAG AGTAGAAACCCTTCCCTCCAGGAGGCCCCCGCTGACTCCCACAGAAACCCACCTACCATAAGATGTCTTCAGGTGGCCTTGTCCAAGGATGGGGGTCAGG CATTTTATATCAAGGGTGCTCTGAACATATTTTATTTTTTAAAAAAACTATGTTTGTGAATTTTGCGTATACTGGCAAGCTTTTGAAAATGTATTTAATT TTGTATTGTTTACCAATGATTTATTTACAAGATATTTACTCAAATAAATGGAGCTGCTTACAAGCCTGTTGACATGTGTGGCTTGCACAACACGTTAA

PPP1R14C.F2 (forward primer)
PPP1R14C.R2 (reverse primer)
Multiple exon skipping

Frame 0

QPGGWARAAQSRCRGALRMRLPGRRW*RRRARS ARPSSGPH*GSGARGHVGGDGQQRDGRRGQRRRR TGFLPKPPGWRRWQPRLQQRLRLLPGGLGARGH GGRCRAGSAATAAATPAGKSDSEIRS*GASEAA GAGGMDRGAAGSALRLRGRRNARGRN*H**SS*C RQ**RESFKITGSSCRLLQTNRDGVSLCCAGWS AVV*SRLTATSAS*VQPILPPQPPE*LGLQTCV TTPG*FLYF**RRGFTMLARLVSNS*PQVFTRLG LPKCWDYRCEPPHPAYRNLSKSCFLG*EA*GN* ALRRRRVYDSGTG*NSPRDEERVLGFVLHEDFC ERIGVLMNNVFVFFFFFFGVKVGGSIRHLFKSVL FLVLKVFVNVIF**QRYHDSSHSLTKDYQRRWT LKEGPRQAAFPARTQMFSTL*YREDSFLTSSLV IFFFLSCTFDSAVKFLTLVYIYTQLS*NVDVSFI SHGWNKLVVVL*LEVPAHVFSRGH*AFFTECC* LQRSLYAPK*KNSVYPLF*CTTPQKC*VGQGLN LWNFYYFIGWDALPGHVLIVSFCSLLKLYEPLI* RTNLISREISTLSQWYFALEKGSTV*Q*IVCFP PSTVFILSPGKQIKRKLADRVSSSLEIGSGSFC AWVVSDQWE*TEC*VSR*ESLTFSQDSCSRS*VA GLEVWRLHFWLLSMACVEDSGCSWP*LCTLIPV LGARPGGVL*PSQLPALWRRIKIQMECGMGNR* E*KPFPPGGPR*LPQKPTYHKMSSGGLVQGWGSG ILYQGCSEHILFFKKTMFVNFAYTGKLLKMYLI LYCLPMIYLQDIYSNKWSCLQAC*HVWLAQHV

Frame 1

SRAAGRARRRAGAGEPFACGCRAGGGSGAGRAP PAPPPGRTEARARGDMSVATGSSETAGGASGGG ARVFFQSPRGGAGGSPGSSSGSGSSREDSAPVAT AAAAGQVQQQQQRRHQQGKVTVKYDRKELRKRL VLEEWIVEQLGQLYGCEEEEMPEVEIDIDDLLD ADSDEERASKLQEALVDCYKPTETESRSVAQVGV QWCDLGSLQPPPPEFNQFSRLSLLSSWDYRRAS PRPANFCIFSRDGVSPCWPGWSQTPDLRYSPAS ASQSAGITGVSHRTRPIGIYQRAAFSDKRHEETE PSAEEECMILEQGETLPETKKESWDLYFMKTFV KE*VSL*TTFLFFFFSFLV*RWGGLLDIYSRAF FFWF*RFLLM*YFNSKDIMTLATA*PRIIKGGGH SRKGHARLRFLQGLRCSVPYDTGKIVFLQVVW* YFFS*VVHLTQLSNFSHLYISTHN*VKMLM*VL FHMDGINLWLSFNWRSQHMCFQEATRHSSLSAAD FNVHFMHQSERIQCIRYFNALHHRNVKLVKGLI YGISIISLVGMLCQVMCLLSHFVVF*SCMNP*F EELT*FLEKYPHYLSGILHWKKEALCSSELSAFH RVLCLFSLQESRSKES*QIECLLP*K*VLVASV PG*YQTSGSKPSVKCQGEKASHSLKTVALGAEL LVWKCGDCISGFSQWLVLRTLGAPGPNCAP*SPC LELGLVACSSPLSYQLFGEGSKFRWNVGWVIGE SRNPSLQEAPADSHRNPPTIRCLQVALSKDGGQ AFYIKGALNIFYFLKKLCL*ILRILASF*KCI*F CIVYQ*FIYKIFTQINGAAYKPVDMCGLHNTL

Frame 2

AGRLGARGAEQVPGSPSHAAAGPEVVAAPGALR PPLLRAALRLGRAGTCRWRRAAARRPAGPAAAA HGFSSKAPGVAPVAAPAPAAAQAPPGRTRRPWP RRPLQGRFSSNSSGDTSREK*Q*NTIVRSFGSGW CWRNGSWSSWVSSTAARKKKCQR*KLTLMIFLM QTVMKRELQNYRKLL*TATNQQRRSLALLRRLE CSGVISAHCNLRLLSSTNSPASAS*VAGITDVRH HARLIFVFLVETGFHHVGQAGLKLLTSGIHPPR PPKVLGLQV*ATAPGLSEFIKELLSRIRGMRKL SPPQKKSV*FWNRVKLSQRRRKSPGICTS*RLL* KNRCPYEQRFCFFFFLFWCEGGGVY*TFIQERS FFGFKGFC*CNILIAKIS*L*PQPNQGLSKEVD TQGRATPGCVSCKDSDVQYLMIQGR*FSYK*FGN IFFLKLYI*LSCQISHTCIYLHTTKLKC*CEFY FTWME*TCGCPLTGGPSTCVFKRPLGILH*VLL TSTFTLCTKVKEFSVSVILMHYTTEMLSWSRA*F MEFLLFHWLGCFARSCAYCLIL*SFKVV*TLNL KN*LDF*RNIHTISVVFCIGKRKHCVAVNCLLS TEYCVYSLSRKADQKKVSRSSVFFLRNRFW*LLC LGSIRPVGVNRVLSVKVRKPHILSRQLL*ELSC WFGSVEIAFLASLNGLC*GLWVLLALIVHPDPR AWS*AWWRALALSVTSSLEKDQNSDGMWDG**VR VETLPSRRPPLTPTETHLP*DVFRWPCPRMGVR HFISRVL*TYFIF*KNYVCEFCVYWQAFENVFN FVLFTNDLFTRYLLK*MELLTSLLTCVACTTR*