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Sequences

Sequence PPP1R14C.bAug05

CAGCCGGGCGGCTGGGCGCGCGCGGCGCAGAGCAGGTGCCGGGGAGCCCTTCGCATGCGGCTGCCGGGCCGGAGGTGGTAGCGGCGCCGGGCGCGCTCCG
CCCGCCCCTCCTCCGGGCCGCACTGAGGCTCGGGCGCGCGGGGACATGTCGGTGGCGACGGGCAGCAGCGAGACGGCCGGCGGGGCCAGCGGCGGCGGCG
CACGGGTTTTCTTCCAAAGCCCCCGGGGTGGCGCCGGTGGCAGCCCCGGCTCCAGCAGCGGCTCAGGCTCCTCCCGGGAGGACTCGGCGCCCGTGGCCAC
GGCGGCCGCTGCAGGGCAGGTTCAGCAGCAACAGCAGCGGCGACACCAGCAGGGAAAAGTGACAGTGAAATACGATCGTAAGGAGCTTCGGAAGCGGCTG
GTGCTGGAGGAATGGATCGTGGAGCAGCTGGGTCAGCTCTACGGCTGCGAGGAAGAAGAAATGCCAGAGGTAGAAATTGACATTGATGATCTTCTTGATG
CAGACAGTGATGAAGAGAGAGCTTCAAAATTACAGGAAGCTCTTGTAGACTGCTACAAACCAACAGAGGAATTTATCAAAGAGCTGCTTTCTCGGATAAG
AGGCATGAGGAAACTGAGCCCTCCGCAGAAGAAGAGTGTATGATTCTGGAACAGGGTGAAACTCTCCCAGAGACGAAGAAAGAGTCCTGGGATTTGTACT
TCATGAAGACTTTTGTGAAAGAATAGGTGTCCTTATGAACAACGTTTTTGTTTTTTTTTTTTTCTTTTTTGGTGTGAAGGTGGGGGGGTCTATTAGACAT
TTATTCAAGAGCGTTCTTTTTTTGGTTTTAAAGGTTTTTGTTAATGTAATATTTTAATAGCAAAGATATCATGACTCTAGCCACAGCCTAACCAAGGATT
ATCAAAGGAGGTGGACACTCAAGGAAGGGCCACGCCAGGCTGCGTTTCCTGCAAGGACTCAGATGTTCAGTACCTTATGATACAGGGAAGATAGTTTTCT
TACAAGTAGTTTGGTAATATTTTTTTTCTTAAGTTGTACATTTGACTCAGCTGTCAAATTTCTCACACTTGTATATATCTACACACAACTAAGTTAAAAT
GTTGATGTGAGTTTTATTTCACATGGATGGAATAAACTTGTGGTTGTCCTTTAACTGGAGGTCCCAGCACATGTGTTTTCAAGAGGCCACTAGGCATTCT
TCACTGAGTGCTGCTGACTTCAACGTTCACTTTATGCACCAAAGTGAAAGAATTCAGTGTATCCGTTATTTTAATGCACTACACCACAGAAATGTTAAGT
TGGTCAAGGGCTTAATTTATGGAATTTCTATTATTTCATTGGTTGGGATGCTTTGCCAGGTCATGTGCTTATTGTCTCATTTTGTAGTCTTTTAAAGTTG
TATGAACCCTTAATTTGAAGAACTAACTTGATTTCTAGAGAAATATCCACACTATCTCAGTGGTATTTTGCATTGGAAAAAGGAAGCACTGTGTAGCAGT
GAATTGTCTGCTTTCCACCGAGTACTGTGTTTATTCTCTCTCCAGGAAAGCAGATCAAAAGAAAGTTAGCAGATCGAGTGTCTTCTTCCTTAGAAATAGG
TTCTGGTAGCTTCTGTGCCTGGGTAGTATCAGACCAGTGGGAGTAAACCGAGTGTTAAGTGTCAAGGTGAGAAAGCCTCACATTCTCTCAAGACAGTTGC
TCTAGGAGCTGAGTTGCTGGTTTGGAAGTGTGGAGATTGCATTTCTGGCTTCTCTCAATGGCTTGTGTTGAGGACTCTGGGTGCTCCTGGCCCTAATTGT
GCACCCTGATCCCCGTGCTTGGAGCTAGGCCTGGTGGCGTGCTCTAGCCCTCTCAGTTACCAGCTCTTTGGAGAAGGATCAAAATTCAGATGGAATGTGG
GATGGGTAATAGGTGAGAGTAGAAACCCTTCCCTCCAGGAGGCCCCCGCTGACTCCCACAGAAACCCACCTACCATAAGATGTCTTCAGGTGGCCTTGTC
CAAGGATGGGGGTCAGGCATTTTATATCAAGGGTGCTCTGAACATATTTTATTTTTTAAAAAAACTATGTTTGTGAATTTTGCGTATACTGGCAAGCTTT
TGAAAATGTATTTAATTTTGTATTGTTTACCAATGATTTATTTACAAGATATTTACTCAAATAAATGGAGCTGCTTACAAGCCTGTTGACATGTGTGGCT
TGCACAACACGTTAA
  • PPP1R14C.F2 (forward primer)
  • PPP1R14C.R2 (reverse primer)
  • Single exon skipping

Frame 0

QPGGWARAAQSRCRGALRMRLPGRRW*RRRARS
ARPSSGPH*GSGARGHVGGDGQQRDGRRGQRRRR
TGFLPKPPGWRRWQPRLQQRLRLLPGGLGARGH
GGRCRAGSAATAAATPAGKSDSEIRS*GASEAA
GAGGMDRGAAGSALRLRGRRNARGRN*H**SS*C
RQ**RESFKITGSSCRLLQTNRGIYQRAAFSDK
RHEETEPSAEEECMILEQGETLPETKKESWDLY
FMKTFVKE*VSL*TTFLFFFFSFLV*RWGGLLDI
YSRAFFFWF*RFLLM*YFNSKDIMTLATA*PRI
IKGGGHSRKGHARLRFLQGLRCSVPYDTGKIVF
LQVVW*YFFS*VVHLTQLSNFSHLYISTHN*VKM
LM*VLFHMDGINLWLSFNWRSQHMCFQEATRHS
SLSAADFNVHFMHQSERIQCIRYFNALHHRNVK
LVKGLIYGISIISLVGMLCQVMCLLSHFVVF*SC
MNP*FEELT*FLEKYPHYLSGILHWKKEALCSS
ELSAFHRVLCLFSLQESRSKES*QIECLLP*K*
VLVASVPG*YQTSGSKPSVKCQGEKASHSLKTVA
LGAELLVWKCGDCISGFSQWLVLRTLGAPGPNC
AP*SPCLELGLVACSSPLSYQLFGEGSKFRWNV
GWVIGESRNPSLQEAPADSHRNPPTIRCLQVALS
KDGGQAFYIKGALNIFYFLKKLCL*ILRILASF
*KCI*FCIVYQ*FIYKIFTQINGAAYKPVDMCG
LHNTL

Frame 1

SRAAGRARRRAGAGEPFACGCRAGGGSGAGRAP
PAPPPGRTEARARGDMSVATGSSETAGGASGGG
ARVFFQSPRGGAGGSPGSSSGSGSSREDSAPVAT
AAAAGQVQQQQQRRHQQGKVTVKYDRKELRKRL
VLEEWIVEQLGQLYGCEEEEMPEVEIDIDDLLD
ADSDEERASKLQEALVDCYKPTEEFIKELLSRIR
GMRKLSPPQKKSV*FWNRVKLSQRRRKSPGICT
S*RLL*KNRCPYEQRFCFFFFLFWCEGGGVY*T
FIQERSFFGFKGFC*CNILIAKIS*L*PQPNQGL
SKEVDTQGRATPGCVSCKDSDVQYLMIQGR*FS
YK*FGNIFFLKLYI*LSCQISHTCIYLHTTKLK
C*CEFYFTWME*TCGCPLTGGPSTCVFKRPLGIL
H*VLLTSTFTLCTKVKEFSVSVILMHYTTEMLS
WSRA*FMEFLLFHWLGCFARSCAYCLIL*SFKV
V*TLNLKN*LDF*RNIHTISVVFCIGKRKHCVAV
NCLLSTEYCVYSLSRKADQKKVSRSSVFFLRNR
FW*LLCLGSIRPVGVNRVLSVKVRKPHILSRQL
L*ELSCWFGSVEIAFLASLNGLC*GLWVLLALIV
HPDPRAWS*AWWRALALSVTSSLEKDQNSDGMW
DG**VRVETLPSRRPPLTPTETHLP*DVFRWPC
PRMGVRHFISRVL*TYFIF*KNYVCEFCVYWQAF
ENVFNFVLFTNDLFTRYLLK*MELLTSLLTCVA
CTTR*

Frame 2

AGRLGARGAEQVPGSPSHAAAGPEVVAAPGALR
PPLLRAALRLGRAGTCRWRRAAARRPAGPAAAA
HGFSSKAPGVAPVAAPAPAAAQAPPGRTRRPWP
RRPLQGRFSSNSSGDTSREK*Q*NTIVRSFGSGW
CWRNGSWSSWVSSTAARKKKCQR*KLTLMIFLM
QTVMKRELQNYRKLL*TATNQQRNLSKSCFLG*
EA*GN*ALRRRRVYDSGTG*NSPRDEERVLGFVL
HEDFCERIGVLMNNVFVFFFFFFGVKVGGSIRH
LFKSVLFLVLKVFVNVIF**QRYHDSSHSLTKD
YQRRWTLKEGPRQAAFPARTQMFSTL*YREDSFL
TSSLVIFFFLSCTFDSAVKFLTLVYIYTQLS*N
VDVSFISHGWNKLVVVL*LEVPAHVFSRGH*AF
FTECC*LQRSLYAPK*KNSVYPLF*CTTPQKC*V
GQGLNLWNFYYFIGWDALPGHVLIVSFCSLLKL
YEPLI*RTNLISREISTLSQWYFALEKGSTV*Q
*IVCFPPSTVFILSPGKQIKRKLADRVSSSLEIG
SGSFCAWVVSDQWE*TEC*VSR*ESLTFSQDSC
SRS*VAGLEVWRLHFWLLSMACVEDSGCSWP*L
CTLIPVLGARPGGVL*PSQLPALWRRIKIQMECG
MGNR*E*KPFPPGGPR*LPQKPTYHKMSSGGLV
QGWGSGILYQGCSEHILFFKKTMFVNFAYTGKL
LKMYLILYCLPMIYLQDIYSNKWSCLQAC*HVWL
AQHV

Sequence PPP1R14C.aAug05

CAGCCGGGCGGCTGGGCGCGCGCGGCGCAGAGCAGGTGCCGGGGAGCCCTTCGCATGCGGCTGCCGGGCCGGAGGTGGTAGCGGCGCCGGGCGCGCTCCG
CCCGCCCCTCCTCCGGGCCGCACTGAGGCTCGGGCGCGCGGGGACATGTCGGTGGCGACGGGCAGCAGCGAGACGGCCGGCGGGGCCAGCGGCGGCGGCG
CACGGGTTTTCTTCCAAAGCCCCCGGGGTGGCGCCGGTGGCAGCCCCGGCTCCAGCAGCGGCTCAGGCTCCTCCCGGGAGGACTCGGCGCCCGTGGCCAC
GGCGGCCGCTGCAGGGCAGGTTCAGCAGCAACAGCAGCGGCGACACCAGCAGGGAAAAGTGACAGTGAAATACGATCGTAAGGAGCTTCGGAAGCGGCTG
GTGCTGGAGGAATGGATCGTGGAGCAGCTGGGTCAGCTCTACGGCTGCGAGGAAGAAGAAATGCCAGAGGTAGAAATTGACATTGATGATCTTCTTGATG
CAGACAGTGATGAAGAGAGAGCTTCAAAATTACAGGAAGCTCTTGTAGACTGCTACAAACCAACAGAGACGGAGTCTCGCTCTGTTGCGCAGGTTGGAGT
GCAGTGGTGTGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGTTCAACCAATTCTCCCGCCTCAGCCTCCTGAGTAGCTGGGATTACAGACGTGCGTCA
CCACGCCCGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTATTCACCCGCCTCGG
CCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCCGGCCTATCGGAATTTATCAAAGAGCTGCTTTCTCGGATAAGAGGCATGAGGAAACTGA
GCCCTCCGCAGAAGAAGAGTGTATGATTCTGGAACAGGGTGAAACTCTCCCAGAGACGAAGAAAGAGTCCTGGGATTTGTACTTCATGAAGACTTTTGTG
AAAGAATAGGTGTCCTTATGAACAACGTTTTTGTTTTTTTTTTTTTCTTTTTTGGTGTGAAGGTGGGGGGGTCTATTAGACATTTATTCAAGAGCGTTCT
TTTTTTGGTTTTAAAGGTTTTTGTTAATGTAATATTTTAATAGCAAAGATATCATGACTCTAGCCACAGCCTAACCAAGGATTATCAAAGGAGGTGGACA
CTCAAGGAAGGGCCACGCCAGGCTGCGTTTCCTGCAAGGACTCAGATGTTCAGTACCTTATGATACAGGGAAGATAGTTTTCTTACAAGTAGTTTGGTAA
TATTTTTTTTCTTAAGTTGTACATTTGACTCAGCTGTCAAATTTCTCACACTTGTATATATCTACACACAACTAAGTTAAAATGTTGATGTGAGTTTTAT
TTCACATGGATGGAATAAACTTGTGGTTGTCCTTTAACTGGAGGTCCCAGCACATGTGTTTTCAAGAGGCCACTAGGCATTCTTCACTGAGTGCTGCTGA
CTTCAACGTTCACTTTATGCACCAAAGTGAAAGAATTCAGTGTATCCGTTATTTTAATGCACTACACCACAGAAATGTTAAGTTGGTCAAGGGCTTAATT
TATGGAATTTCTATTATTTCATTGGTTGGGATGCTTTGCCAGGTCATGTGCTTATTGTCTCATTTTGTAGTCTTTTAAAGTTGTATGAACCCTTAATTTG
AAGAACTAACTTGATTTCTAGAGAAATATCCACACTATCTCAGTGGTATTTTGCATTGGAAAAAGGAAGCACTGTGTAGCAGTGAATTGTCTGCTTTCCA
CCGAGTACTGTGTTTATTCTCTCTCCAGGAAAGCAGATCAAAAGAAAGTTAGCAGATCGAGTGTCTTCTTCCTTAGAAATAGGTTCTGGTAGCTTCTGTG
CCTGGGTAGTATCAGACCAGTGGGAGTAAACCGAGTGTTAAGTGTCAAGGTGAGAAAGCCTCACATTCTCTCAAGACAGTTGCTCTAGGAGCTGAGTTGC
TGGTTTGGAAGTGTGGAGATTGCATTTCTGGCTTCTCTCAATGGCTTGTGTTGAGGACTCTGGGTGCTCCTGGCCCTAATTGTGCACCCTGATCCCCGTG
CTTGGAGCTAGGCCTGGTGGCGTGCTCTAGCCCTCTCAGTTACCAGCTCTTTGGAGAAGGATCAAAATTCAGATGGAATGTGGGATGGGTAATAGGTGAG
AGTAGAAACCCTTCCCTCCAGGAGGCCCCCGCTGACTCCCACAGAAACCCACCTACCATAAGATGTCTTCAGGTGGCCTTGTCCAAGGATGGGGGTCAGG
CATTTTATATCAAGGGTGCTCTGAACATATTTTATTTTTTAAAAAAACTATGTTTGTGAATTTTGCGTATACTGGCAAGCTTTTGAAAATGTATTTAATT
TTGTATTGTTTACCAATGATTTATTTACAAGATATTTACTCAAATAAATGGAGCTGCTTACAAGCCTGTTGACATGTGTGGCTTGCACAACACGTTAA
  • PPP1R14C.F2 (forward primer)
  • PPP1R14C.R2 (reverse primer)
  • Single exon skipping

Frame 0

QPGGWARAAQSRCRGALRMRLPGRRW*RRRARS
ARPSSGPH*GSGARGHVGGDGQQRDGRRGQRRRR
TGFLPKPPGWRRWQPRLQQRLRLLPGGLGARGH
GGRCRAGSAATAAATPAGKSDSEIRS*GASEAA
GAGGMDRGAAGSALRLRGRRNARGRN*H**SS*C
RQ**RESFKITGSSCRLLQTNRDGVSLCCAGWS
AVV*SRLTATSAS*VQPILPPQPPE*LGLQTCV
TTPG*FLYF**RRGFTMLARLVSNS*PQVFTRLG
LPKCWDYRCEPPHPAYRNLSKSCFLG*EA*GN*
ALRRRRVYDSGTG*NSPRDEERVLGFVLHEDFC
ERIGVLMNNVFVFFFFFFGVKVGGSIRHLFKSVL
FLVLKVFVNVIF**QRYHDSSHSLTKDYQRRWT
LKEGPRQAAFPARTQMFSTL*YREDSFLTSSLV
IFFFLSCTFDSAVKFLTLVYIYTQLS*NVDVSFI
SHGWNKLVVVL*LEVPAHVFSRGH*AFFTECC*
LQRSLYAPK*KNSVYPLF*CTTPQKC*VGQGLN
LWNFYYFIGWDALPGHVLIVSFCSLLKLYEPLI*
RTNLISREISTLSQWYFALEKGSTV*Q*IVCFP
PSTVFILSPGKQIKRKLADRVSSSLEIGSGSFC
AWVVSDQWE*TEC*VSR*ESLTFSQDSCSRS*VA
GLEVWRLHFWLLSMACVEDSGCSWP*LCTLIPV
LGARPGGVL*PSQLPALWRRIKIQMECGMGNR*
E*KPFPPGGPR*LPQKPTYHKMSSGGLVQGWGSG
ILYQGCSEHILFFKKTMFVNFAYTGKLLKMYLI
LYCLPMIYLQDIYSNKWSCLQAC*HVWLAQHV

Frame 1

SRAAGRARRRAGAGEPFACGCRAGGGSGAGRAP
PAPPPGRTEARARGDMSVATGSSETAGGASGGG
ARVFFQSPRGGAGGSPGSSSGSGSSREDSAPVAT
AAAAGQVQQQQQRRHQQGKVTVKYDRKELRKRL
VLEEWIVEQLGQLYGCEEEEMPEVEIDIDDLLD
ADSDEERASKLQEALVDCYKPTETESRSVAQVGV
QWCDLGSLQPPPPEFNQFSRLSLLSSWDYRRAS
PRPANFCIFSRDGVSPCWPGWSQTPDLRYSPAS
ASQSAGITGVSHRTRPIGIYQRAAFSDKRHEETE
PSAEEECMILEQGETLPETKKESWDLYFMKTFV
KE*VSL*TTFLFFFFSFLV*RWGGLLDIYSRAF
FFWF*RFLLM*YFNSKDIMTLATA*PRIIKGGGH
SRKGHARLRFLQGLRCSVPYDTGKIVFLQVVW*
YFFS*VVHLTQLSNFSHLYISTHN*VKMLM*VL
FHMDGINLWLSFNWRSQHMCFQEATRHSSLSAAD
FNVHFMHQSERIQCIRYFNALHHRNVKLVKGLI
YGISIISLVGMLCQVMCLLSHFVVF*SCMNP*F
EELT*FLEKYPHYLSGILHWKKEALCSSELSAFH
RVLCLFSLQESRSKES*QIECLLP*K*VLVASV
PG*YQTSGSKPSVKCQGEKASHSLKTVALGAEL
LVWKCGDCISGFSQWLVLRTLGAPGPNCAP*SPC
LELGLVACSSPLSYQLFGEGSKFRWNVGWVIGE
SRNPSLQEAPADSHRNPPTIRCLQVALSKDGGQ
AFYIKGALNIFYFLKKLCL*ILRILASF*KCI*F
CIVYQ*FIYKIFTQINGAAYKPVDMCGLHNTL

Frame 2

AGRLGARGAEQVPGSPSHAAAGPEVVAAPGALR
PPLLRAALRLGRAGTCRWRRAAARRPAGPAAAA
HGFSSKAPGVAPVAAPAPAAAQAPPGRTRRPWP
RRPLQGRFSSNSSGDTSREK*Q*NTIVRSFGSGW
CWRNGSWSSWVSSTAARKKKCQR*KLTLMIFLM
QTVMKRELQNYRKLL*TATNQQRRSLALLRRLE
CSGVISAHCNLRLLSSTNSPASAS*VAGITDVRH
HARLIFVFLVETGFHHVGQAGLKLLTSGIHPPR
PPKVLGLQV*ATAPGLSEFIKELLSRIRGMRKL
SPPQKKSV*FWNRVKLSQRRRKSPGICTS*RLL*
KNRCPYEQRFCFFFFLFWCEGGGVY*TFIQERS
FFGFKGFC*CNILIAKIS*L*PQPNQGLSKEVD
TQGRATPGCVSCKDSDVQYLMIQGR*FSYK*FGN
IFFLKLYI*LSCQISHTCIYLHTTKLKC*CEFY
FTWME*TCGCPLTGGPSTCVFKRPLGILH*VLL
TSTFTLCTKVKEFSVSVILMHYTTEMLSWSRA*F
MEFLLFHWLGCFARSCAYCLIL*SFKVV*TLNL
KN*LDF*RNIHTISVVFCIGKRKHCVAVNCLLS
TEYCVYSLSRKADQKKVSRSSVFFLRNRFW*LLC
LGSIRPVGVNRVLSVKVRKPHILSRQLL*ELSC
WFGSVEIAFLASLNGLC*GLWVLLALIVHPDPR
AWS*AWWRALALSVTSSLEKDQNSDGMWDG**VR
VETLPSRRPPLTPTETHLP*DVFRWPCPRMGVR
HFISRVL*TYFIF*KNYVCEFCVYWQAFENVFN
FVLFTNDLFTRYLLK*MELLTSLLTCVACTTR*

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