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Sequences

Sequence BMX.fAug05

TAGCAGCAGCAGTGAAGATGTTGCAAGCAGTAACCAAAAAGAAAGAAATGTGAATCACACCACCTCAAAGATTTCATGGGAATTCCCTGAGTCAAGTTCA TCTGAAGAAGAGGAAAACCTGGATGATTATGACTGGTTTGCTGGTAACATCTCCAGATCACAATCTGAACAGTTACTCAGACAAAAGGGAAAAGAAGGAG CATTTATGGTTAGAAATTCGAGCCAAGTGGGAATGTACACAGTGTCCTTATTTAGTAAGGCTGTGAAGCATGATCACACGGCTCCGCCACCCTGTGTCAA CAAAGGCCAACAAGGTCCCCGACTCTGTGTCCCTGGGAAATGGAATCTGGGAACTGAAAAGAGAAGAGATTACCTTGTTGAAGGAGCTGGGAAGTGGCCA GTTTGGAGTGGTCCAGCTGGGCAAGTGGAAGGGGCAGTATGATGTTGCTGTTAAGATGATCAAGGAGGGCTCCATGTCAGAAGATGAATTCTTTCAGGAG GCCCAGACTATGAT

BMX.F2 (forward primer)
BMX.R2 (reverse primer)
Single exon skipping

Frame 0

*QQQ*RCCKQ*PKRKKCESHHLKDFMGIP*VKF I*RRGKPG*L*LVCW*HLQITI*TVTQTKGKRRS IYG*KFEPSGNVHSVLI**GCEA*SHGSATLCQ QRPTRSPTLCPWEMESGN*KEKRLPC*RSWEVA SLEWSSWASGRGSMMLLLR*SRRAPCQKMNSFRR PRL*

Frame 1

SSSSEDVASSNQKERNVNHTTSKISWEFPESSS SEEEENLDDYDWFAGNISRSQSEQLLRQKGKEG AFMVRNSSQVGMYTVSLFSKAVKHDHTAPPPCVN KGQQGPRLCVPGKWNLGTEKRRDYLVEGAGKWP VWSGPAGQVEGAV*CCC*DDQGGLHVRR*ILSG GPDYD

Frame 2

AAAVKMLQAVTKKKEM*ITPPQRFHGNSLSQVH LKKRKTWMIMTGLLVTSPDHNLNSYSDKREKKE HLWLEIRAKWECTQCPYLVRL*SMITRLRHPVS TKANKVPDSVSLGNGIWELKREEITLLKELGSGQ FGVVQLGKWKGQYDVAVKMIKEGSMSEDEFFQE AQTM

Sequence BMX.aAug05

AGCATAGAGCTCTACAAACAAAGGCAGGGTTATCACATGTACCCGAGAGCTGCCTGGGAAAAGTGGGAGCTATAGTTATCAAAATTGGAAACTTTATGAA GTATGCTTTTGTCTCCGTTTTTGTTGCGTTCAATCAGATCAACAAGTGTTGGCTAAGTGTTGAGCGTGGTTTCCTGTGGGATTGTGTAAGGCTCTGTAGG ACAATAAACGTGTTGGGGGCACTGAGTAATGTAGCCATTTCTGACCCGGCAGCCAGGAAAATGTGAAACAATTTGCTTCTGGAAACAGGACAGCCGGGGC CGTGTTCCTGCAACAGCAGACCAAGCACCGCGGCGGACCCAGGCAAGCACGGAACAAGCTGAGACGGATGATAATATGGATACAAAATCTATTCTAGAAG AACTTCTTCTCAAAAGATCACAGCAAAAGAAGAAAATGTCACCAAATAATTACAAAGAACGGCTTTTTGTTTTGACCAAAACAAACCTTTCCTACTATGA ATATGACAAAATGAAAAGGGGCAGCAGAAAAGGATCCATTGAAATTAAGAAAATCAGATGTGTGGAGAAAGTAAATCTCGAGGAGCAGACGCCTGTAGAG AGACAGTACCCATTTCAGATTGTCTATAAAGATGGGCTTCTCTATGTCTATGCATCAAATGAAGAGAGCCGAAGTCAGTGGTTGAAAGCATTACAAAAAG AGATAAGGGGTAACCCCCACCTGCTGGTCAAGTACCATAGTGGGTTCTTCGTGGACGGGAAGTTCCTGTGTTGCCAGCAGAGCTGTAAAGCAGCCCCAGG ATGTACCCTCTGGGAAGCATATGCTAATCTGCATACTGCAGTCAATGAAGAGAAACACAGAGTTCCCACCTTCCCAGACAGAGTGCTGAAGATACCTCGG GCAGTTCCTGTTCTCAAAATGGATGCACCATCTTCAAGTACCACTCTAGCCCAATATGACAACGAATCAAAGAAAAACTATGGCTCCCAGCCACCATCTT CAAGTACCAGTCTAGCGCAATATGACAGCAACTCAAAGAAAATCTATGGCTCCCAGCCAAACTTCAACATGCAGTATATTCCAAGGGAAGACTTCCCTGA CTGGTGGCAAGTAAGAAAACTGAAAAGTAGCAGCAGCAGTGAAGATGTTGCAAGCAGTAACCAAAAAGAAAGAAATGTGAATCACACCACCTCAAAGATT TCATGGGAATTCCCTGAGTCAAGTTCATCTGAAGAAGAGGAAAACCTGGATGATTATGACTGGTTTGCTGGTAACATCTCCAGATCACAATCTGAACAGT TACTCAGACAAAAGGGAAAAGAAGGAGCATTTATGGTTAGAAATTCGAGCCAAGTGGGAATGTACACAGTGTCCTTATTTAGTAAGGCTGTGAATGATAA AAAAGGAACTGTCAAACATTACCACGTGCATACAAATGCTGAGAACAAATTATACCTGGCAGAAAACTACTGTTTTGATTCCATTCCAAAGCTTATTCAT TATCATCAACACAATTCAGCAGGCATGATCACACGGCTCCGCCACCCTGTGTCAACAAAGGCCAACAAGGTCCCCGACTCTGTGTCCCTGGGAAATGGAA TCTGGGAACTGAAAAGAGAAGAGATTACCTTGTTGAAGGAGCTGGGAAGTGGCCAGTTTGGAGTGGTCCAGCTGGGCAAGTGGAAGGGGCAGTATGATGT TGCTGTTAAGATGATCAAGGAGGGCTCCATGTCAGAAGATGAATTCTTTCAGGAGGCCCAGACTATGATGAAACTCAGCCATCCCAAGCTGGTTAAATTC TATGGAGTGTGTTCAAAGGAATACCCCATATACATAGTGACTGAATATATAAGCAATGGCTGCTTGCTGAATTACCTGAGGAGTCACGGAAAAGGACTTG AACCTTCCCAGCTCTTAGAAATGTGCTACGATGTCTGTGAAGGCATGGCCTTCTTGGAGAGTCACCAATTCATACACCGGGACTTGGCTGCTCGTAACTG CTTGGTGGACAGAGATCTCTGTGTGAAAGTATCTGACTTTGGAATGACAAGGTATGTTCTTGATGACCAGTATGTCAGTTCAGTCGGAACAAAGTTTCCA GTCAAGTGGTCAGCTCCAGAGGTGTTTCATTACTTCAAATACAGCAGCAAGTCAGACGTATGGGCATTTGGGATCCTGATGTGGGAGGTGTTCAGCCTGG GGAAGCAGCCCTATGACTTGTATGACAACTCCCAGGTGGTTCTGAAGGTCTCCCAGGGCCACAGGCTTTACCGGCCCCACCTGGCATCGGACACCATCTA CCAGATCATGTACAGCTGCTGGCACGAGCTTCCAGAAAAGCGTCCCACATTTCAGCAACTCCTGTCTTCCATTGAACCACTTCGGGAAAAAGACAAGCAT TGAAGAAGAAATTAGGAGTGCTGATAAGAATGAATATAGATGCTGGCCAGCATTTTCATTCATTTTAAGGAAAGTAGCAAGGCATAATGTAATTTAGCTA GTTTTTAATAGTGTTCTCTGTATTGTCTATTATTTAGAAATGAACAAGGCAGGAAACAAAAGATTCCCTTGAAATTTAGATCAAATTAGTAATTTTGTTT ATGCTGCTCCTGATATAACACTTTCCAGCCTATAGCAGAAGCACATTTTCAGACTGCAATATAGAGACTGTGTTCATGTGTAAAGACTGAGCAGAACTGA AAAATTACTTATTGGATATTCATTCTTTTCTTTATATTGTCATTGTCACAACAATTAAATATACTACCAAGTACAGAAATGTGGA

BMX.F2 (forward primer)
BMX.R2 (reverse primer)
Single exon skipping

Frame 0

SIELYKQRQGYHMYPRAAWEKWEL*LSKLETL* SMLLSPFLLRSIRSTSVG*VLSVVSCGIV*GSVG Q*TCWGH*VM*PFLTRQPGKCETICFWKQDSRG RVPATADQAPRRTQASTEQAETDDNMDTKSILE ELLLKRSQQKKKMSPNNYKERLFVLTKTNLSYYE YDKMKRGSRKGSIEIKKIRCVEKVNLEEQTPVE RQYPFQIVYKDGLLYVYASNEESRSQWLKALQK EIRGNPHLLVKYHSGFFVDGKFLCCQQSCKAAPG CTLWEAYANLHTAVNEEKHRVPTFPDRVLKIPR AVPVLKMDAPSSSTTLAQYDNESKKNYGSQPPS SSTSLAQYDSNSKKIYGSQPNFNMQYIPREDFPD WWQVRKLKSSSSSEDVASSNQKERNVNHTTSKI SWEFPESSSSEEEENLDDYDWFAGNISRSQSEQ LLRQKGKEGAFMVRNSSQVGMYTVSLFSKAVNDK KGTVKHYHVHTNAENKLYLAENYCFDSIPKLIH YHQHNSAGMITRLRHPVSTKANKVPDSVSLGNG IWELKREEITLLKELGSGQFGVVQLGKWKGQYDV AVKMIKEGSMSEDEFFQEAQTMMKLSHPKLVKF YGVCSKEYPIYIVTEYISNGCLLNYLRSHGKGL EPSQLLEMCYDVCEGMAFLESHQFIHRDLAARNC LVDRDLCVKVSDFGMTRYVLDDQYVSSVGTKFP VKWSAPEVFHYFKYSSKSDVWAFGILMWEVFSL GKQPYDLYDNSQVVLKVSQGHRLYRPHLASDTIY QIMYSCWHELPEKRPTFQQLLSSIEPLREKDKH *RRN*EC**E*I*MLASIFIHFKESSKA*CNLA SF**CSLYCLLFRNEQGRKQKIPLKFRSN**FCL CCS*YNTFQPIAEAHFQTAI*RLCSCVKTEQN* KITYWIFILFFILSLSQQLNILPSTEMW

Frame 1

A*SSTNKGRVITCTRELPGKSGSYSYQNWKLYE VCFCLRFCCVQSDQQVLAKC*AWFPVGLCKAL* DNKRVGGTE*CSHF*PGSQENVKQFASGNRTAGA VFLQQQTKHRGGPRQARNKLRRMIIWIQNLF*K NFFSKDHSKRRKCHQIITKNGFLF*PKQTFPTM NMTK*KGAAEKDPLKLRKSDVWRK*ISRSRRL*R DSTHFRLSIKMGFSMSMHQMKRAEVSG*KHYKK R*GVTPTCWSSTIVGSSWTGSSCVASRAVKQPQ DVPSGKHMLICILQSMKRNTEFPPSQTEC*RYLG QFLFSKWMHHLQVPL*PNMTTNQRKTMAPSHHL QVPV*RNMTATQRKSMAPSQTSTCSIFQGKTSL TGGK*EN*KVAAAVKMLQAVTKKKEM*ITPPQRF HGNSLSQVHLKKRKTWMIMTGLLVTSPDHNLNS YSDKREKKEHLWLEIRAKWECTQCPYLVRL*MI KKELSNITTCIQMLRTNYTWQKTTVLIPFQSLFI IINTIQQA*SHGSATLCQQRPTRSPTLCPWEME SGN*KEKRLPC*RSWEVASLEWSSWASGRGSMM LLLR*SRRAPCQKMNSFRRPRL**NSAIPSWLNS MECVQRNTPYT**LNI*AMAAC*IT*GVTEKDL NLPSS*KCATMSVKAWPSWRVTNSYTGTWLLVT AWWTEISV*KYLTLE*QGMFLMTSMSVQSEQSFQ SSGQLQRCFITSNTAASQTYGHLGS*CGRCSAW GSSPMTCMTTPRWF*RSPRATGFTGPTWHRTPS TRSCTAAGTSFQKSVPHFSNSCLPLNHFGKKTSI EEEIRSADKNEYRCWPAFSFILRKVARHNVI*L VFNSVLCIVYYLEMNKAGNKRFP*NLDQISNFV YAAPDITLSSL*QKHIFRLQYRDCVHV*RLSRTE KLLIGYSFFSLYCHCHNN*IYYQVQKCG

Frame 2

HRALQTKAGLSHVPESCLGKVGAIVIKIGNFMK YAFVSVFVAFNQINKCWLSVERGFLWDCVRLCR TINVLGALSNVAISDPAARKM*NNLLLETGQPG PCSCNSRPSTAADPGKHGTS*DG**YGYKIYSRR TSSQKITAKEENVTK*LQRTAFCFDQNKPFLL* I*QNEKGQQKRIH*N*ENQMCGESKSRGADACR ETVPISDCL*RWASLCLCIK*REPKSVVESITKR DKG*PPPAGQVP*WVLRGREVPVLPAEL*SSPR MYPLGSIC*SAYCSQ*RETQSSHLPRQSAEDTS GSSCSQNGCTIFKYHSSPI*QRIKEKLWLPATIF KYQSSAI*QQLKENLWLPAKLQHAVYSKGRLP* LVASKKTEK*QQQ*RCCKQ*PKRKKCESHHLKD FMGIP*VKFI*RRGKPG*L*LVCW*HLQITI*TV TQTKGKRRSIYG*KFEPSGNVHSVLI**GCE** KRNCQTLPRAYKC*EQIIPGRKLLF*FHSKAYS LSSTQFSRHDHTAPPPCVNKGQQGPRLCVPGKWN LGTEKRRDYLVEGAGKWPVWSGPAGQVEGAV*C CC*DDQGGLHVRR*ILSGGPDYDETQPSQAG*I LWSVFKGIPHIHSD*IYKQWLLAELPEESRKRT* TFPALRNVLRCL*RHGLLGESPIHTPGLGCS*L LGGQRSLCESI*LWNDKVCS**PVCQFSRNKVS SQVVSSRGVSLLQIQQQVRRMGIWDPDVGGVQPG EAAL*LV*QLPGGSEGLPGPQALPAPPGIGHHL PDHVQLLARASRKASHISATPVFH*TTSGKRQA LKKKLGVLIRMNIDAGQHFHSF*GK*QGIM*FS* FLIVFSVLSII*K*TRQETKDSLEI*IKLVILF MLLLI*HFPAYSRSTFSDCNIETVFMCKD*AEL KNYLLDIHSFLYIVIVTTIKYTTKYRNV

Sequence BMX.bAug05

TGGAGGTGCCCGGATGAACATACATCACAATGTGAACAGTGGTCATCTCCAGATAGTAGAATTTCAGATATGTTTCTTTCCATTTTTCTGACAGTTTGAA TTTTCTGTAATAATTAATTGACTTTTATACAATGGAAACAACTTTTTTGTTTTGGAAAAAAGAAAGATGCTGCCGCTAATCAGTGGATGAAAGATGATAA TATGGATACAAAATCTATTCTAGAAGAACTTCTTCTCAAAAGATCACAGCAAAAGAAGAAAATGTCACCAAATAATTACAAAGAACGGCTTTTTGTTTTG ACCAAAACAAACCTTTCCTACTATGAATATGACAAAATGAAAAGGGGCAGCAGAAAAGGATCCATTGAAATTAAGAAAATCAGATGTGTGGAGAAAGTAA ATCTCGAGGAGCAGACGCCTGTAGAGAGACAGTACCCATTTCAGATTGTCTATAAAGATGGGCTTCTCTATGTCTATGCATCAAATGAAGAGAGCCGAAG TCAGTGGTTGAAAGCATTACAAAAAGAGATAAGGGGTAACCCCCACCTGCTGGTCAAGTACCATAGTGGGTTCTTCGTGGACGGGAAGTTCCTGTGTTGC CAGCAGAGCTGTAAAGCAGCCCCAGGATGTACCCTCTGGGAAGCATATGCTAATCTGCATACTGCAGTCAATGAAGAGAAACACAGAGTTCCCACCTTCC CAGACAGAGTGCTGAAGATACCTCGGGCAGTTCCTGTTCTCAAAATGGATGCACCATCTTCAAGTACCACTCTAGCCCAATATGACAACGAATCAAAGAA AAACTATGGCTCCCAGCCACCATCTTCAAGTACCAGTCTAGCGCAATATGACAGCAACTCAAAGAAAATCTATGGCTCCCAGCCAAACTTCAACATGCAG TATATTCCAAGGGAAGACTTCCCTGACTGGTGGCAAGTAAGAAAACTGAAAAGTAGCAGCAGCAGTGAAGATGTTGCAAGCAGTAACCAAAAAGAAAGAA ATGTGAATCACACCACCTCAAAGATTTCATGGGAATTCCCTGAGTCAAGTTCATCTGAAGAAGAGGAAAACCTGGATGATTATGACTGGTTTGCTGGTAA CATCTCCAGATCACAATCTGAACAGTTACTCAGACAAAAGGGAAAAGAAGGAGCATTTATGGTTAGAAATTCGAGCCAAGTGGGAATGTACACAGTGTCC TTATTTAGTAAGGCTGTGAATGATAAAAAAGGAACTGTCAAACATTACCACGTGCATACAAATGCTGAGAACAAATTATACCTGGCAGAAAACTACTGTT TTGATTCCATTCCAAAGCTTATTCATTATCATCAACACAATTCAGCAGGCATGATCACACGGCTCCGCCACCCTGTGTCAACAAAGGCCAACAAGGTCCC CGACTCTGTGTCCCTGGGAAATGGAATCTGGGAACTGAAAAGAGAAGAGATTACCTTGTTGAAGGAGCTGGGAAGTGGCCAGTTTGGAGTGGTCCAGCTG GGCAAGTGGAAGGGGCAGTATGATGTTGCTGTTAAGATGATCAAGGAGGGCTCCATGTCAGAAGATGAATTCTTTCAGGAGGCCCAGACTATGATGAAAC TCAGCCATCCCAAGCTGGTTAAATTCTATGGAGTGTGTTCAAAGGAATACCCCATATACATAGTGACTGAATATATAAGCAATGGCTGCTTGCTGAATTA CCTGAGGAGTCACGGAAAAGGACTTGAACCTTCCCAGCTCTTAGAAATGTGCTACGATGTCTGTGAAGGCATGGCCTTCTTGGAGAGTCACCAATTCATA CACCGGGACTTGGCTGCTCGTAACTGCTTGGTGGACAGAGATCTCTGTGTGAAAGTATCTGACTTTGGAATGACAAGGTATGTTCTTGATGACCAGTATG TCAGTTCAGTCGGAACAAAGTTTCCAGTCAAGTGGTCAGCTCCAGAGGTGTTTCATTACTTCAAATACAGCAGCAAGTCAGACGTATGGGCATTTGGGAT CCTGATGTGGGAGGTGTTCAGCCTGGGGAAGCAGCCCTATGACTTGTATGACAACTCCCAGGTGGTTCTGAAGGTCTCCCAGGGCCACAGGCTTTACCGG CCCCACCTGGCATCGGACACCATCTACCAGATCATGTACAGCTGCTGGCACGAGCTTCCAGAAAAGCGTCCCACATTTCAGCAACTCCTGTCTTCCATTG AACCACTTCGGGAAAAAGACAAGCATTGAAGAAGAAATTAGGAGTGCTGATAAGAATGAATATAGATGCTGGCCAGCATTTTCATTCATTTTAAGGAAAG TAGCAAGGCATAATGTAATTTAGCTAGTTTTTAATAGTGTTCTCTGTATTGTCTATTATTTAGAAATGAACAAGGCAGGAAACAAAAGATTCCCTTGAAA TTTAGATCAAATTAGTAATTTTGTTTATGCTGCTCCTGATATAACACTTTCCAGCCTATAGCAGAAGCACATTTTCAGACTGCAATATAGAGACTGTGTT CATGTGTAAAGACTGAGCAGAACTGAAAAATTACTTATTGGATATTCATTCTTTTCTTTATATTGTCATTGTCACAACAATTAAATATACTACCAAGTAC AGAAATGTGGA

BMX.F2 (forward primer)
BMX.R2 (reverse primer)
Single exon skipping

Frame 0

WRCPDEHTSQCEQWSSPDSRISDMFLSIFLTV* IFCNN*LTFIQWKQLFCFGKKKDAAANQWMKDDN MDTKSILEELLLKRSQQKKKMSPNNYKERLFVL TKTNLSYYEYDKMKRGSRKGSIEIKKIRCVEKV NLEEQTPVERQYPFQIVYKDGLLYVYASNEESRS QWLKALQKEIRGNPHLLVKYHSGFFVDGKFLCC QQSCKAAPGCTLWEAYANLHTAVNEEKHRVPTF PDRVLKIPRAVPVLKMDAPSSSTTLAQYDNESKK NYGSQPPSSSTSLAQYDSNSKKIYGSQPNFNMQ YIPREDFPDWWQVRKLKSSSSSEDVASSNQKER NVNHTTSKISWEFPESSSSEEEENLDDYDWFAGN ISRSQSEQLLRQKGKEGAFMVRNSSQVGMYTVS LFSKAVNDKKGTVKHYHVHTNAENKLYLAENYC FDSIPKLIHYHQHNSAGMITRLRHPVSTKANKVP DSVSLGNGIWELKREEITLLKELGSGQFGVVQL GKWKGQYDVAVKMIKEGSMSEDEFFQEAQTMMK LSHPKLVKFYGVCSKEYPIYIVTEYISNGCLLNY LRSHGKGLEPSQLLEMCYDVCEGMAFLESHQFI HRDLAARNCLVDRDLCVKVSDFGMTRYVLDDQY VSSVGTKFPVKWSAPEVFHYFKYSSKSDVWAFGI LMWEVFSLGKQPYDLYDNSQVVLKVSQGHRLYR PHLASDTIYQIMYSCWHELPEKRPTFQQLLSSI EPLREKDKH*RRN*EC**E*I*MLASIFIHFKES SKA*CNLASF**CSLYCLLFRNEQGRKQKIPLK FRSN**FCLCCS*YNTFQPIAEAHFQTAI*RLC SCVKTEQN*KITYWIFILFFILSLSQQLNILPST EMW

Frame 1

GGARMNIHHNVNSGHLQIVEFQICFFPFF*QFE FSVIIN*LLYNGNNFFVLEKRKMLPLISG*KMI IWIQNLF*KNFFSKDHSKRRKCHQIITKNGFLF* PKQTFPTMNMTK*KGAAEKDPLKLRKSDVWRK* ISRSRRL*RDSTHFRLSIKMGFSMSMHQMKRAE VSG*KHYKKR*GVTPTCWSSTIVGSSWTGSSCVA SRAVKQPQDVPSGKHMLICILQSMKRNTEFPPS QTEC*RYLGQFLFSKWMHHLQVPL*PNMTTNQR KTMAPSHHLQVPV*RNMTATQRKSMAPSQTSTCS IFQGKTSLTGGK*EN*KVAAAVKMLQAVTKKKE M*ITPPQRFHGNSLSQVHLKKRKTWMIMTGLLV TSPDHNLNSYSDKREKKEHLWLEIRAKWECTQCP YLVRL*MIKKELSNITTCIQMLRTNYTWQKTTV LIPFQSLFIIINTIQQA*SHGSATLCQQRPTRS PTLCPWEMESGN*KEKRLPC*RSWEVASLEWSSW ASGRGSMMLLLR*SRRAPCQKMNSFRRPRL**N SAIPSWLNSMECVQRNTPYT**LNI*AMAAC*I T*GVTEKDLNLPSS*KCATMSVKAWPSWRVTNSY TGTWLLVTAWWTEISV*KYLTLE*QGMFLMTSM SVQSEQSFQSSGQLQRCFITSNTAASQTYGHLG S*CGRCSAWGSSPMTCMTTPRWF*RSPRATGFTG PTWHRTPSTRSCTAAGTSFQKSVPHFSNSCLPL NHFGKKTSIEEEIRSADKNEYRCWPAFSFILRK VARHNVI*LVFNSVLCIVYYLEMNKAGNKRFP*N LDQISNFVYAAPDITLSSL*QKHIFRLQYRDCV HV*RLSRTEKLLIGYSFFSLYCHCHNN*IYYQV QKCG

Frame 2

EVPG*TYITM*TVVISR**NFRYVSFHFSDSLN FL**LIDFYTMETTFLFWKKERCCR*SVDER** YGYKIYSRRTSSQKITAKEENVTK*LQRTAFCF DQNKPFLL*I*QNEKGQQKRIH*N*ENQMCGESK SRGADACRETVPISDCL*RWASLCLCIK*REPK SVVESITKRDKG*PPPAGQVP*WVLRGREVPVL PAEL*SSPRMYPLGSIC*SAYCSQ*RETQSSHLP RQSAEDTSGSSCSQNGCTIFKYHSSPI*QRIKE KLWLPATIFKYQSSAI*QQLKENLWLPAKLQHA VYSKGRLP*LVASKKTEK*QQQ*RCCKQ*PKRKK CESHHLKDFMGIP*VKFI*RRGKPG*L*LVCW* HLQITI*TVTQTKGKRRSIYG*KFEPSGNVHSV LI**GCE**KRNCQTLPRAYKC*EQIIPGRKLLF *FHSKAYSLSSTQFSRHDHTAPPPCVNKGQQGP RLCVPGKWNLGTEKRRDYLVEGAGKWPVWSGPA GQVEGAV*CCC*DDQGGLHVRR*ILSGGPDYDET QPSQAG*ILWSVFKGIPHIHSD*IYKQWLLAEL PEESRKRT*TFPALRNVLRCL*RHGLLGESPIH TPGLGCS*LLGGQRSLCESI*LWNDKVCS**PVC QFSRNKVSSQVVSSRGVSLLQIQQQVRRMGIWD PDVGGVQPGEAAL*LV*QLPGGSEGLPGPQALP APPGIGHHLPDHVQLLARASRKASHISATPVFH* TTSGKRQALKKKLGVLIRMNIDAGQHFHSF*GK *QGIM*FS*FLIVFSVLSII*K*TRQETKDSLE I*IKLVILFMLLLI*HFPAYSRSTFSDCNIETVF MCKD*AELKNYLLDIHSFLYIVIVTTIKYTTKY RNV

Sequence BMX.cAug05

CCTGGCATAGCCAGAGGGAATATGAGTGATGGTGCCTCAAAGCAGTAACTTTTTGCTTAGAGCTTGAGAGTCAAAGTTAAGGACCCACATGTATACTTCG GCTCTAGCGAGTCTAAGGATGATAATATGGATACAAAATCTATTCTAGAAGAACTTCTTCTCAAAAGATCACAGCAAAAGAAGAAAATGTCACCAAATAA TTACAAAGAACGGCTTTTTGTTTTGACCAAAACAAACCTTTCCTACTATGAATATGACAAAATGAAAAGGGGCAGCAGAAAAGGATCCATTGAAATTAAG AAAATCAGATGTGTGGAGAAAGTAAATCTCGAGGAGCAGACGCCTGTAGAGAGACAGTACCCATTTCAGATTGTCTATAAAGATGGGCTTCTCTATGTCT ATGCATCAAATGAAGAGAGCCGAAGTCAGTGGTTGAAAGCATTACAAAAAGAGATAAGGGGTAACCCCCACCTGCTGGTCAAGTACCATAGTGGGTTCTT CGTGGACGGGAAGTTCCTGTGTTGCCAGCAGAGCTGTAAAGCAGCCCCAGGATGTACCCTCTGGGAAGCATATGCTAATCTGCATACTGCAGTCAATGAA GAGAAACACAGAGTTCCCACCTTCCCAGACAGAGTGCTGAAGATACCTCGGGCAGTTCCTGTTCTCAAAATGGATGCACCATCTTCAAGTACCACTCTAG CCCAATATGACAACGAATCAAAGAAAAACTATGGCTCCCAGCCACCATCTTCAAGTACCAGTCTAGCGCAATATGACAGCAACTCAAAGAAAATCTATGG CTCCCAGCCAAACTTCAACATGCAGTATATTCCAAGGGAAGACTTCCCTGACTGGTGGCAAGTAAGAAAACTGAAAAGTAGCAGCAGCAGTGAAGATGTT GCAAGCAGTAACCAAAAAGAAAGAAATGTGAATCACACCACCTCAAAGATTTCATGGGAATTCCCTGAGTCAAGTTCATCTGAAGAAGAGGAAAACCTGG ATGATTATGACTGGTTTGCTGGTAACATCTCCAGATCACAATCTGAACAGTTACTCAGACAAAAGGGAAAAGAAGGAGCATTTATGGTTAGAAATTCGAG CCAAGTGGGAATGTACACAGTGTCCTTATTTAGTAAGGCTGTGAATGATAAAAAAGGAACTGTCAAACATTACCACGTGCATACAAATGCTGAGAACAAA TTATACCTGGCAGAAAACTACTGTTTTGATTCCATTCCAAAGCTTATTCATTATCATCAACACAATTCAGCAGGCATGATCACACGGCTCCGCCACCCTG TGTCAACAAAGGCCAACAAGGTCCCCGACTCTGTGTCCCTGGGAAATGGAATCTGGGAACTGAAAAGAGAAGAGATTACCTTGTTGAAGGAGCTGGGAAG TGGCCAGTTTGGAGTGGTCCAGCTGGGCAAGTGGAAGGGGCAGTATGATGTTGCTGTTAAGATGATCAAGGAGGGCTCCATGTCAGAAGATGAATTCTTT CAGGAGGCCCAGACTATGATGAAACTCAGCCATCCCAAGCTGGTTAAATTCTATGGAGTGTGTTCAAAGGAATACCCCATATACATAGTGACTGAATATA TAAGCAATGGCTGCTTGCTGAATTACCTGAGGAGTCACGGAAAAGGACTTGAACCTTCCCAGCTCTTAGAAATGTGCTACGATGTCTGTGAAGGCATGGC CTTCTTGGAGAGTCACCAATTCATACACCGGGACTTGGCTGCTCGTAACTGCTTGGTGGACAGAGATCTCTGTGTGAAAGTATCTGACTTTGGAATGACA AGGTATGTTCTTGATGACCAGTATGTCAGTTCAGTCGGAACAAAGTTTCCAGTCAAGTGGTCAGCTCCAGAGGTGTTTCATTACTTCAAATACAGCAGCA AGTCAGACGTATGGGCATTTGGGATCCTGATGTGGGAGGTGTTCAGCCTGGGGAAGCAGCCCTATGACTTGTATGACAACTCCCAGGTGGTTCTGAAGGT CTCCCAGGGCCACAGGCTTTACCGGCCCCACCTGGCATCGGACACCATCTACCAGATCATGTACAGCTGCTGGCACGAGCTTCCAGAAAAGCGTCCCACA TTTCAGCAACTCCTGTCTTCCATTGAACCACTTCGGGAAAAAGACAAGCATTGAAGAAGAAATTAGGAGTGCTGATAAGAATGAATATAGATGCTGGCCA GCATTTTCATTCATTTTAAGGAAAGTAGCAAGGCATAATGTAATTTAGCTAGTTTTTAATAGTGTTCTCTGTATTGTCTATTATTTAGAAATGAACAAGG CAGGAAACAAAAGATTCCCTTGAAATTTAGATCAAATTAGTAATTTTGTTTATGCTGCTCCTGATATAACACTTTCCAGCCTATAGCAGAAGCACATTTT CAGACTGCAATATAGAGACTGTGTTCATGTGTAAAGACTGAGCAGAACTGAAAAATTACTTATTGGATATTCATTCTTTTCTTTATATTGTCATTGTCAC AACAATTAAATATACTACCAAGTACAGAAATGTGGA

BMX.F2 (forward primer)
BMX.R2 (reverse primer)
Single exon skipping

Frame 0

PGIARGNMSDGASKQ*LFA*SLRVKVKDPHVYF GSSESKDDNMDTKSILEELLLKRSQQKKKMSPNN YKERLFVLTKTNLSYYEYDKMKRGSRKGSIEIK KIRCVEKVNLEEQTPVERQYPFQIVYKDGLLYV YASNEESRSQWLKALQKEIRGNPHLLVKYHSGFF VDGKFLCCQQSCKAAPGCTLWEAYANLHTAVNE EKHRVPTFPDRVLKIPRAVPVLKMDAPSSSTTL AQYDNESKKNYGSQPPSSSTSLAQYDSNSKKIYG SQPNFNMQYIPREDFPDWWQVRKLKSSSSSEDV ASSNQKERNVNHTTSKISWEFPESSSSEEEENL DDYDWFAGNISRSQSEQLLRQKGKEGAFMVRNSS QVGMYTVSLFSKAVNDKKGTVKHYHVHTNAENK LYLAENYCFDSIPKLIHYHQHNSAGMITRLRHP VSTKANKVPDSVSLGNGIWELKREEITLLKELGS GQFGVVQLGKWKGQYDVAVKMIKEGSMSEDEFF QEAQTMMKLSHPKLVKFYGVCSKEYPIYIVTEY ISNGCLLNYLRSHGKGLEPSQLLEMCYDVCEGMA FLESHQFIHRDLAARNCLVDRDLCVKVSDFGMT RYVLDDQYVSSVGTKFPVKWSAPEVFHYFKYSS KSDVWAFGILMWEVFSLGKQPYDLYDNSQVVLKV SQGHRLYRPHLASDTIYQIMYSCWHELPEKRPT FQQLLSSIEPLREKDKH*RRN*EC**E*I*MLA SIFIHFKESSKA*CNLASF**CSLYCLLFRNEQG RKQKIPLKFRSN**FCLCCS*YNTFQPIAEAHF QTAI*RLCSCVKTEQN*KITYWIFILFFILSLS QQLNILPSTEMW

Frame 1

LA*PEGI*VMVPQSSNFLLRA*ESKLRTHMYTS ALASLRMIIWIQNLF*KNFFSKDHSKRRKCHQI ITKNGFLF*PKQTFPTMNMTK*KGAAEKDPLKLR KSDVWRK*ISRSRRL*RDSTHFRLSIKMGFSMS MHQMKRAEVSG*KHYKKR*GVTPTCWSSTIVGS SWTGSSCVASRAVKQPQDVPSGKHMLICILQSMK RNTEFPPSQTEC*RYLGQFLFSKWMHHLQVPL* PNMTTNQRKTMAPSHHLQVPV*RNMTATQRKSM APSQTSTCSIFQGKTSLTGGK*EN*KVAAAVKML QAVTKKKEM*ITPPQRFHGNSLSQVHLKKRKTW MIMTGLLVTSPDHNLNSYSDKREKKEHLWLEIR AKWECTQCPYLVRL*MIKKELSNITTCIQMLRTN YTWQKTTVLIPFQSLFIIINTIQQA*SHGSATL CQQRPTRSPTLCPWEMESGN*KEKRLPC*RSWE VASLEWSSWASGRGSMMLLLR*SRRAPCQKMNSF RRPRL**NSAIPSWLNSMECVQRNTPYT**LNI *AMAAC*IT*GVTEKDLNLPSS*KCATMSVKAW PSWRVTNSYTGTWLLVTAWWTEISV*KYLTLE*Q GMFLMTSMSVQSEQSFQSSGQLQRCFITSNTAA SQTYGHLGS*CGRCSAWGSSPMTCMTTPRWF*R SPRATGFTGPTWHRTPSTRSCTAAGTSFQKSVPH FSNSCLPLNHFGKKTSIEEEIRSADKNEYRCWP AFSFILRKVARHNVI*LVFNSVLCIVYYLEMNK AGNKRFP*NLDQISNFVYAAPDITLSSL*QKHIF RLQYRDCVHV*RLSRTEKLLIGYSFFSLYCHCH NN*IYYQVQKCG

Frame 2

WHSQREYE*WCLKAVTFCLELESQS*GPTCILR L*RV*G**YGYKIYSRRTSSQKITAKEENVTK* LQRTAFCFDQNKPFLL*I*QNEKGQQKRIH*N* ENQMCGESKSRGADACRETVPISDCL*RWASLCL CIK*REPKSVVESITKRDKG*PPPAGQVP*WVL RGREVPVLPAEL*SSPRMYPLGSIC*SAYCSQ* RETQSSHLPRQSAEDTSGSSCSQNGCTIFKYHSS PI*QRIKEKLWLPATIFKYQSSAI*QQLKENLW LPAKLQHAVYSKGRLP*LVASKKTEK*QQQ*RC CKQ*PKRKKCESHHLKDFMGIP*VKFI*RRGKPG *L*LVCW*HLQITI*TVTQTKGKRRSIYG*KFE PSGNVHSVLI**GCE**KRNCQTLPRAYKC*EQ IIPGRKLLF*FHSKAYSLSSTQFSRHDHTAPPPC VNKGQQGPRLCVPGKWNLGTEKRRDYLVEGAGK WPVWSGPAGQVEGAV*CCC*DDQGGLHVRR*IL SGGPDYDETQPSQAG*ILWSVFKGIPHIHSD*IY KQWLLAELPEESRKRT*TFPALRNVLRCL*RHG LLGESPIHTPGLGCS*LLGGQRSLCESI*LWND KVCS**PVCQFSRNKVSSQVVSSRGVSLLQIQQQ VRRMGIWDPDVGGVQPGEAAL*LV*QLPGGSEG LPGPQALPAPPGIGHHLPDHVQLLARASRKASH ISATPVFH*TTSGKRQALKKKLGVLIRMNIDAGQ HFHSF*GK*QGIM*FS*FLIVFSVLSII*K*TR QETKDSLEI*IKLVILFMLLLI*HFPAYSRSTF SDCNIETVFMCKD*AELKNYLLDIHSFLYIVIVT TIKYTTKYRNV

Sequence BMX.dAug05

ACTTTGAGAGTAGCAGCAGCAGTGAAGATGTTGCAAGCAGTAACCAAAAAGAAAGAAATGTGAATCACACCACCTCAAAGATTTCATGGGAATTCCCTGA GTCAAGTTCATCTGAAGAAGAGGAAAACCTGGATGATTATGACTGGTTTGCTGGTAACATCTCCAGATCACAATCTGAACAGTTACTCAGACAAAAGGGA AAAGAAGGAGCATTTATGGTTAGAAATTCGAGCCAAGTGGGAATGTACACAGTGTCCTTATTTAGTAAGGCTGTGAATGATAAAAAAGGAACTGTCAAAC ATTACCACGTGCATACAAATGCTGAGAACAAATTATACCTGGCAGAAAACTACTGTTTTGATTCCATTCCAAAGCTTATTCATTATCATCAACACAATTC AGCAGGCATGATCACACGGCTCCGCCACCCTGTGTCAACAAAGGCCAACAAGGTCCCCGACTCTGTGTCCCTGGGAAATGGAATCTGGGAACTGAAAAGA GAAGAGATTACCTTGTTGAAGGAGCTGGGAAGTGGCCAGTTTGGAGTGGTCCAGCTGGGCAAGTGGAAGGGGCAGTATGATGTTGCTGTTAAGATGATCA AGGAGGGCTCCATGTCAGAAGATGAATTCTTTCAGGAGGCCCAGACTATGATGAAACTCAGCCATCCCAAGCTGGTTAAATTCTATGGAGTGTGTTCAAA GGAATACCCCATATACATAGTGACTGAATATATAAGCAATGGCTGCTTGCTGAATTACCTGAGGAGTCACGGAAAAGGACTTGAACCTTCCCAGCTCTTA GAAATGTGCTACGATGTCTGTGAAGGCATGGCC

BMX.F2 (forward primer)
BMX.R2 (reverse primer)
Single exon skipping

Frame 0

TLRVAAAVKMLQAVTKKKEM*ITPPQRFHGNSL SQVHLKKRKTWMIMTGLLVTSPDHNLNSYSDKRE KKEHLWLEIRAKWECTQCPYLVRL*MIKKELSN ITTCIQMLRTNYTWQKTTVLIPFQSLFIIINTI QQA*SHGSATLCQQRPTRSPTLCPWEMESGN*KE KRLPC*RSWEVASLEWSSWASGRGSMMLLLR*S RRAPCQKMNSFRRPRL**NSAIPSWLNSMECVQ RNTPYT**LNI*AMAAC*IT*GVTEKDLNLPSS* KCATMSVKAW

Frame 1

L*E*QQQ*RCCKQ*PKRKKCESHHLKDFMGIP* VKFI*RRGKPG*L*LVCW*HLQITI*TVTQTKG KRRSIYG*KFEPSGNVHSVLI**GCE**KRNCQT LPRAYKC*EQIIPGRKLLF*FHSKAYSLSSTQF SRHDHTAPPPCVNKGQQGPRLCVPGKWNLGTEK RRDYLVEGAGKWPVWSGPAGQVEGAV*CCC*DDQ GGLHVRR*ILSGGPDYDETQPSQAG*ILWSVFK GIPHIHSD*IYKQWLLAELPEESRKRT*TFPAL RNVLRCL*RHG

Frame 2

FESSSSSEDVASSNQKERNVNHTTSKISWEFPE SSSSEEEENLDDYDWFAGNISRSQSEQLLRQKG KEGAFMVRNSSQVGMYTVSLFSKAVNDKKGTVK HYHVHTNAENKLYLAENYCFDSIPKLIHYHQHNS AGMITRLRHPVSTKANKVPDSVSLGNGIWELKR EEITLLKELGSGQFGVVQLGKWKGQYDVAVKMI KEGSMSEDEFFQEAQTMMKLSHPKLVKFYGVCSK EYPIYIVTEYISNGCLLNYLRSHGKGLEPSQLL EMCYDVCEGMA