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Sequences

Sequence CHN2.gAug05

CCAGACCCACAGGGCAAAAAGTGCGTCGTCGTGTCCCAACCTCCTGGTCCCAGAAACCTGGCCGCATCAAGTCAGCGCTTCCCATGCTGGAAGAAGCAAG
CAG
CCCCAGGGTGGAATCTTAAAAATTAATGAAGAGCATCGGCGGGGTGCCATTCAGATGCTGAAGAATACCAGCCTCCTATATGGAAATCATACTTATA
TCAGTTACAGCAAGAGGCACCTCGTCCCAAGAGAATCATTTGTCCTCGGGAGGTGGAAAACAGACCAAAATATTATGGAAGAGAGTTTCATGGGATCATC
TCTCGGGAGCAGGCGGATGAGCTTCTTGGAGGCGTGGAGGGTGCCTACATCCTTAGAGAAAGCCAGCGGCAACCAGGATGCTACACGCTGGCTCTCAGGT
TTGGAAACCAGACCTTAAACTACAGGCTCTTCCACGACGGGAAACACTTTGTGGGTGAGAAGAGGTTTGAGTCGATTCATGATCTGGTGACAGATGGCTT
GATAACACTGTACATAGAAACAAAAGCTGCCGAGTACATTTCAAAAATGACAACTAACCCCATCTATGAACACATTGGATATGCCACCCTACTCAGAGAA
AAAGTATCCAGAAGGCTGAGCAGGTCTAAAAATGAACCAAGAAAAACAAACGTCACACATGAAGAACACACAGCGGTGGAAAAGGTGAGCTGTGTGTGAT
GGAAGCAGCCTTTCGTTTTAATCCATGCCGCATCAACAGGCGGGTTAACTATAGGTGCGATTTGCTGAAATGTTGGAGGAGACCCACCCCCACCCGAAGA
ACTCTAGAATGTTAGGGCCAAAGGGACCTTAGAAATCTTCTAGCTCAGGCCGGGCGTGGTGGCTCATGCCTGTAATCTCTTTGGGAGGCCGAGGCAGGTG
GATCACGAGGTCAGGAGATAGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTACAAAAAATACAAAAAATCAGCCGGGTGTGGTGGTGGGCGCCT
GTAGTCCCAGTTACTTGGGAGGCTGAGGCAGGAGAATGGCATGAACCCAGGACGGGGAGCTTGCAGTGAGCCAAGATTGTGCCACTGCACTCCAGTCTGG
GCAACAGAGCGAGACTCCGTCTC
  • CHN2.F1 (forward primer)
  • CHN2.R1 (reverse primer)
  • Single exon skipping

Frame 0

PDPQGKKCVVVSQPPGPRNLAASSQRFPCWKKQ
A
APGWNLKN**RASAGCHSDAEEYQPPIWKSYLY
QLQQEAPRPKRIICPREVENRPKYYGREFHGII
SREQADELLGGVEGAYILRESQRQPGCYTLALR
FGNQTLNYRLFHDGKHFVGEKRFESIHDLVTDGL
ITLYIETKAAEYISKMTTNPIYEHIGYATLLRE
KVSRRLSRSKNEPRKTNVTHEEHTAVEKVSCV*
WKQPFVLIHAASTGGLTIGAIC*NVGGDPPPPEE
L*NVRAKGTLEIF*LRPGVVAHACNLFGRPRQV
DHEVRR*RPSWLTR*NPVSTKNTKNQPGVVVGA
CSPSYLGG*GRRMA*TQDGELAVSQDCATALQSG
QQSETPS

Frame 1

QTHRAKSASSCPNLLVPETWPHQVSASHAGRSK
Q
PQGGILKINEEHRRGAIQMLKNTSLLYGNHTY
ISYSKRHLVPRESFVLGRWKTDQNIMEESFMGSS
LGSRRMSFLEAWRVPTSLEKASGNQDATRWLSG
LETRP*TTGSSTTGNTLWVRRGLSRFMIW*QMA
**HCT*KQKLPSTFQK*QLTPSMNTLDMPPYSEK
KYPEG*AGLKMNQEKQTSHMKNTQRWKR*AVCD
GSSLSF*SMPHQQAG*L*VRFAEMLEETHPHPK
NSRMLGPKGP*KSSSSGRAWWLMPVISLGGRGRW
ITRSGDRDHPG*HGETPSLQKIQKISRVWWWAP
VVPVTWEAEAGEWHEPRTGSLQ*AKIVPLHSSL
GNRARLRL

Frame 2

RPTGQKVRRRVPTSWSQKPGRIKSALPMLEEAS
S
PRVES*KLMKSIGGVPFRC*RIPASYMEIILI
SVTARGTSSQENHLSSGGGKQTKILWKRVSWDH
LSGAGG*ASWRRGGCLHP*RKPAATRMLHAGSQV
WKPDLKLQALPRRETLCG*EEV*VDS*SGDRWL
DNTVHRNKSCRVHFKNDN*PHL*THWICHPTQR
KSIQKAEQV*K*TKKNKRHT*RTHSGGKGELCVM
EAAFRFNPCRINRRVNYRCDLLKCWRRPTPTRR
TLEC*GQRDLRNLLAQAGRGGSCL*SLWEAEAG
GSRGQEIETILANTVKPRLYKKYKKSAGCGGGRL
*SQLLGRLRQENGMNPGRGACSEPRLCHCTPVW
ATERDSV

Sequence CHN2.dAug05

CCAGACCCACAGGGCAAAAAGTGCGTCGTCGTGTCCCAACCTCCTGGTCCCAGAAACCTGGCCGCATCAAGTCAGCGCTTCCCATGCTGGAAGAAGCAAG
CAG
CCCCAGGGTGGAATCTTAAAAATTAATGAAGAGCATCGGCGGGGTGCCATTCAGGACTTACTTGCAAGCCCAGGATTTACATTTGGAAAGCGAGTGG
TGTTTGATTCCCACTGTTTAAAAAGGCAACACACGTTCGCTGATGGTCTACATTCCAGCTGCACTAGCA
ATGCTGAAGAATACCAGCCTCCTATATGGAA
ATCA
TACTTATATCAGTTACAGCAAGAGGCACCTCGTCCCAAGAGAATCATTTGTCCTCGGGAGGTGGAAAACAGACCAAAATATTATGGAAGAGAGTTT
CATGGGATCATCTCTCGGGAGCAGGCGGATGAGCTTCTTGGAGGCGTGGAGGGTGCCTACATCCTTAGAGAAAGCCAGCGGCAACCAGGATGCTACACGC
TGGCTCTCAGGTTTGGAAACCAGACCTTAAACTACAGGCTCTTCCACGACGGGAAACACTTTGTGGGTGAGAAGAGGTTTGAGTCGATTCATGATCTGGT
GACAGATGGCTTGATAACACTGTACATAGAAACAAAAGCTGCCGAGTACATTTCAAAAATGACAACTAACCCCATCTATGAACACATTGGATATGCCACC
CTACTCAGAGAAAAAGTATCCAGAAGGCTGAGCAGGTCTAAAAATGAACCAAGAAAAACAAACGTCACACATGAAGAACACACAGCGGTGGAAAAGGTGA
GCTGTGTGTGATGGAAGCAGCCTTTCGTTTTAATCCATGCCGCATCAACAGGCGGGTTAACTATAGGTGCGATTTGCTGAAATGTTGGAGGAGACCCACC
CCCACCCGAAGAACTCTAGAATGTTAGGGCCAAAGGGACCTTAGAAATCTTCTAGCTCAGGCCGGGCGTGGTGGCTCATGCCTGTAATCTCTTTGGGAGG
CCGAGGCAGGTGGATCACGAGGTCAGGAGATAGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTACAAAAAATACAAAAAATCAGCCGGGTGTGG
TGGTGGGCGCCTGTAGTCCCAGTTACTTGGGAGGCTGAGGCAGGAGAATGGCATGAACCCAGGACGGGGAGCTTGCAGTGAGCCAAGATTGTGCCACTGC
ACTCCAGTCTGGGCAACAGAGCGAGACTCCGTCTC
  • CHN2.F1 (forward primer)
  • CHN2.R1 (reverse primer)
  • Single exon skipping

Frame 0

PDPQGKKCVVVSQPPGPRNLAASSQRFPCWKKQ
A
APGWNLKN**RASAGCHSGLTCKPRIYIWKASG
V*FPLFKKATHVR*WSTFQLH*Q
C*RIPASYME
I
ILISVTARGTSSQENHLSSGGGKQTKILWKRV
SWDHLSGAGG*ASWRRGGCLHP*RKPAATRMLHA
GSQVWKPDLKLQALPRRETLCG*EEV*VDS*SG
DRWLDNTVHRNKSCRVHFKNDN*PHL*THWICH
PTQRKSIQKAEQV*K*TKKNKRHT*RTHSGGKGE
LCVMEAAFRFNPCRINRRVNYRCDLLKCWRRPT
PTRRTLEC*GQRDLRNLLAQAGRGGSCL*SLWE
AEAGGSRGQEIETILANTVKPRLYKKYKKSAGCG
GGRL*SQLLGRLRQENGMNPGRGACSEPRLCHC
TPVWATERDSV

Frame 1

QTHRAKSASSCPNLLVPETWPHQVSASHAGRSK
Q
PQGGILKINEEHRRGAIQDLLASPGFTFGKRV
VFDSHCLKRQHTFADGLHSSCTS
NAEEYQPPIWK
S
YLYQLQQEAPRPKRIICPREVENRPKYYGREF
HGIISREQADELLGGVEGAYILRESQRQPGCYT
LALRFGNQTLNYRLFHDGKHFVGEKRFESIHDLV
TDGLITLYIETKAAEYISKMTTNPIYEHIGYAT
LLREKVSRRLSRSKNEPRKTNVTHEEHTAVEKV
SCV*WKQPFVLIHAASTGGLTIGAIC*NVGGDPP
PPEEL*NVRAKGTLEIF*LRPGVVAHACNLFGR
PRQVDHEVRR*RPSWLTR*NPVSTKNTKNQPGV
VVGACSPSYLGG*GRRMA*TQDGELAVSQDCATA
LQSGQQSETPS

Frame 2

RPTGQKVRRRVPTSWSQKPGRIKSALPMLEEAS
S
PRVES*KLMKSIGGVPFRTYLQAQDLHLESEW
CLIPTV*KGNTRSLMVYIPAALA
MLKNTSLLYG
NH
TYISYSKRHLVPRESFVLGRWKTDQNIMEESF
MGSSLGSRRMSFLEAWRVPTSLEKASGNQDATR
WLSGLETRP*TTGSSTTGNTLWVRRGLSRFMIW
*QMA**HCT*KQKLPSTFQK*QLTPSMNTLDMPP
YSEKKYPEG*AGLKMNQEKQTSHMKNTQRWKR*
AVCDGSSLSF*SMPHQQAG*L*VRFAEMLEETH
PHPKNSRMLGPKGP*KSSSSGRAWWLMPVISLGG
RGRWITRSGDRDHPG*HGETPSLQKIQKISRVW
WWAPVVPVTWEAEAGEWHEPRTGSLQ*AKIVPL
HSSLGNRARLRL

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