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Sequences

Sequence RUNX2.bAug05

GTGTGAATGCTTCATTCGCCTCACAAACAACCACAGAACCACAAGTGCGGTGCAAACTTTCTCCAGGAGGACAGCAAGAAGTCTCTGGTTTTTAAATGGT TAATCTCCGCAGGTCACTACCAGCCACCGAGACCAACAGAGTCATTTAAGGCTGCAAGCAGTATTTACAACAGAGGGTACAAGTTCTATCTGAAAAAAAA AGGAGGGACTATGGCATCAAACAGCCTCTTCAGCACAGTGACACCATGTCAGCAAAACTTCTTTTGGGATCCGAGCACCAGCCGGCGCTTCAGCCCCCCC TCCAGCAGCCTGCAGCCCGGCAAAATGAGCGACGTGAGCCCGGTGGTGGCTGCGCAACAGCAGCAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGCAGC AGCAACAGCAGCAGCAGCAGCAGGAGGCGGCGGCGGCGGCTGCGGCGGCGGCGGCGGCTGCGGCGGCGGCAGCTGCAGTGCCCCGGTTGCGGCCGCCCCA CGACAACCGCACCATGGTGGAGATCATCGCCGACCACCCGGCCGAACTCGTCCGCACCGACAGCCCCAACTTCCTGTGCTCGGTGCTGCCCTCGCACTGG CGCTGCAACAAGACCCTGCCCGTGGCCTTCAAGGTGGTAGCCCTCGGAGAGGTACCAGATGGGACTGTGGTTACTGTCATGGCGGGTAACGATGAAAATT ATTCTGCTGAGCTCCGGAATGCCTCTGCTGTTATGAAAAACCAAGTAGCAAGGTTCAACGATCTGAGATTTGTGGGCCGGAGTGGACGAGGCAAGAGTTT CACCTTGACCATAACCGTCTTCACAAATCCTCCCCAAGTAGCTACCTATCACAGAGCAATTAAAGTTACAGTAGATGGACCTCGGGAACCCAGAAGGCAC AGACAGAAGCTTGATGACTCTAAACCTAGTTTGTTCTCTGACCGCCTCAGTGATTTAGGGCGCATTCCTCATCCCAGTATGAGAGTAGGTGTCCCGCCTC AGAACCCACGGCCCTCCCTGAACTCTGCACCAAGTCCTTTTAATCCACAAGGACAGAGTCAGATTACAGACCCCAGGCAGGCACAGTCTTCCCCGCCGTG GTCCTATGACCAGTCTTACCCCTCCTACCTGAGCCAGATGACGTCCCCGTCCATCCACTCTACCACCCCGCTGTCTTCCACACGGGGCACTGGGCTTCCT GCCATCACCGATGTGCCTAGGCGCATTTCAGGTGCTTCAGAACTGGGCCCTTTTTCAGACCCCAGGCAGTTCCCAAGCATTTCATCCCTCACTGAGAGCC GCTTCTCCAACCCACGAATGCACTATCCAGCCACCTTTACTTACACCCCGCCAGTCACCTCAGGCATGTCCCTCGGTATGTCCGCCACCACTCACTACCA CACCTACCTGCCACCACCCTACCCCGGCTCTTCCCAAAGCCAGAGTGGACCCTTCCAGACCAGCAGCACTCCATATCTCTACTATGGCACTTCGTCAGGA TCCTATCAGTTTCCCATGGTGCCGGGGGGAGACCGGTCTCCTTCCAGAATGCTTCCGCCATGCACCACCACCTCGAATGGCAGCACGCTATTAAATCCAA ATTTGCCTAACCAGAATGATGGTGTTGACGCTGATGGAAGCCACAGCAGTTCCCCAACTGTTTTGAATTCTAGTGGCAGAATGGATGAATCTGTTTGGCG ACCATATTGAAATTCCTCAGCAGTGGCCCAGTGGTATCTGGGGGCCACATCCCACACGTATCAATATATACATATATAGAGAGAGTGCATATATATGTAT ATCGATTAGCTATCTACAAAGTGCCTATTTTTTAGAAGATTTTTCATTCACTCACTCAGTCATGATCTTGCAGCCATAAGAGGGTAGATATTGAGAAGCA GAAGGCTCAAGAGAGACAATTGCAATCGAGCTTCAGATTGTTTACTATTTAAGATGTACTTTTACAAAGGAACAAAGAAGGGAAAAGGTATTTTTGTTTT TGTTGTTTGGTCTGTTATCATCAATAACCTGTTCATATGCCAATTCAGAGAGGTGGACTCCAGGTTCAGGAGGGAGAAGAGCAAAGCCGCTTCCTCTCTG TGCTTTGAAACTTCACACCCTCACGGTGGCAGCTGTGTATGGACCAGTGCCCTCCGCAGACAGCTCACAAAACCAGTTGAGGTGCACTAAAGGGACATGA GGTAGAATGGATGCTTCCATCACAGTACCATCATTCAGAATAACTCTTCCAATTTCTGCTTTCAGACATGCTGCAGGTCCTCATCTGAACTGTTGGGTTC GTTTTTTTTTTTTTTTTTCCTGCTCCAAGAAAGTGACTTCAAAAATAACTGATCAGGATAGATTATTTTATTTTACTTTTTAACACTCCTTCTCCCCTTT TCCCACTGAACCAAAAAGAAATCCCATCCCTAAAACCTGCCTTCTCCTTTTATGCAAAACTGAAAATGGCAATACATTATTATAGCCATAATGGTATAGA TAGTGATTGCGTTTGGCTATGTGTTGTTTTCTTTTTTTTTAAATTATGAATATGTGTAAAATCTGAGGTAACTTGCTAACGTGAATGGTCATATAACTTT AAAGATATATTTATAATTATTTAATGACATTTGGACCCTTGAAACATTTCTTAGTGTATTGATATGTTGACTTCGGTCTCTAAAAGTGCTCTTTATTAAA TAACAAATTTCTTCAGTGGTCTAGAGCCATATCTGAAATATTGCTAAGCAATTTCAGTTCATCCAGGCACAATGTGATTTTAAAAAATACTTCCATCTCC AAATATTTTAGATATAGATTGTTTTTGTGATGTATGAAGGAAATGTTATGTTTAGTTCTTTCAGATCTTTGAATGCCTCTAACACAGCTTTGCCTTCTAA AGCGGTAATTAGGGATTTAAAAAACAACCTTTAGCCCTTTATCAGCATGAAATGCTGGAGTGATGTGGTTTTCTAATTTCTTTGGGGTAATTATGACTCT TGTCATATTAAAAAGACAAGCACAAGTAAATCATTGAACTACAGAAAAATGTTCTGTGGTTTCATAGTTAAGCAAAACTCTAAATCGCCAGGCTTCATAG CAAAGACATAGTCAGCTAAAAGCCGCACATGTGGATAGAGGGTTCAATTATGAGACACCTAGTACAGGAGAGCAAAATTGCACCAGAGATTCTTAACCAA CCAGCCTTACCAAACAACACAACAGGGGAACCCCAATCTGCCTTACCCAAGGCCCCACTGGCAGCTTTCCACAGAATTTGCATTTAGAGGAGCAGAATGA CATCACTGTCCTTTGGGAGTAGGTCCTCTGAAAAGGCAGCAGGTTCCAGCAGGTAGCTGAGCTGAGAGGACATATGGCCCACGGGGACCTACAGACAGCC TTTGACATTTGTATTTCTTACAATGGAGGGCCAAGGAGGGCAAGGGGCTGTGGAGTTTGGTGTCTACTAGTGTGTATGAATTTGAGCTAGAGTCCTTCTG TGGCATGCACTTTGACCACTCCTGGCAGTCACATGGCAGATTTCCAAGTGCAAATCCTTAATCCAAACAAGGATCATCTAATGACACCACCAGGCCAATC CCTGCTCTCCTCCCCGAAAAGTCAGGGTCCCTTCATTGGAATCCTCCACCCACCCAAGCAGAATTTAGCAGAGATTTGCCTTCAAACCCTAACGGCCCCC TTGTTCTCTGGTCCTTCTCAAACCCACCTTTGTAGGCCACCCAGCATTGCAGGACAGCGTGTGGGGCAGCTGGACCTGTGCTTCCTGCCTGGGAGTCTCC CTTGGAATTCATCCTGACTCCTTCTAATAAAAATGGATGGGAAAGCAAAACACTTTGCCTTCTAAAGGCCGTATACCAAGTATGCTTAGATAAATAAGCC ACTTTTCTATTACTTAAGTAAGAAGGAAGTAGTAATTGATACTATTTATTGTTTGTGTGTGGTAGCTTGAAGCACACCACTGTCCATTTATTTGTAAGTG TAAAATATGTGTGTTTGTTTCAGCAGCACTTAAAAAAGCCAGTGTCTGGTTACACATTTCAATTTTAATTAATTGACATAAAAATGCTACCGCCAGTGCC AGCTGCATCCTATTTAATTAAAAAGGTACTATATTTGTACATTATTTTTTAATGTTAAAAGGGCTTTTTTAAGTTTACAGTACACATACCGAGTGACTTT AGGGATGCTTTTGTGTTGAAATGTTACTATAGTGGCTGCAGGCAGCAACCCAGAAACACTTTAGAAGCTTTTTTTCCTTGGGAAAAATTCAAGCACTTCT TCCCTCCACCCTCACTCCAACCACCCCAATGGGGGTAATTCACATTTCTTAGAACAAATTCTGCCCTTTTTTGGTCTAGGGATTAAAATTTTGTTTTTCT TTCTTTCTTTTTTTTTTTTTTTCACTGAACCCTTAATTTGCACTGGGTCATGTGTTTGATTTGTGATTTCAAGACCAAAGCAAAGTCTTACTACTACTGT GGAACCATGTACTAGTTCCTGGGAATTAAAATAGCGTGGTTCTCTTTGTAGCACAAACATTGCTGGAATTTGCAGTCTTTTCAATGCAGCCACATTTTTA TCCATTTCAGTTGTCTCACAAATTTTAACCCATATCAGAGTTCCAGAACAGGTACCACAGCTTTGGTTTTAGATTAGTGGAATAACATTCAGCCCAGAAC TGAGAAACTCAACAGATTAACTATCGTTTGCTCTTTAGACGGTCTCACTGCCTCTCACTTGCCAGAGCCCTTTCAAAATGAGCAGAGAAGTCCACACCAT TAGGGACCATCTGTGATAAATTCAGAAGGGAGGAGATGTGTGTACAGCTTTAAGGATTCCCTCAATTCCGAGGAAAGGGACTGGCCCAGAATCCAGGTTA ATACATGGAAACACGAAGCATTAGCAAAAGTAATAATTATACCTATGGTATTTGAAAGAACAATAATAAAAGACACTTCTTCCAAACCTTGAATTTGTTG TTTTTAGAAAACGAATGCATTTAAAAATATTTTCTATGTGAGAATTTTTTAGATGTGTGTTTACTTCATGTTTACAAATAACTGTTTGCTTTTTAATGCA GTACTTTGAAATATATCAGCCAAAACCATAACTTACAATAATTTCTTAGGTATTCTGAATAAAATTCCATTTCTTTTGGATATGCTTTACCATTCTTAGG TTTCTGTGGAACAAAAATATTTGTAGCATTTTGTGTAAATACAAGCTTTCATTTTTATTTTTTCCAATTGCTATTGCCCAAGAATTGCTTTCCATGCACA TATTGTAAAAATTCCGCTTTGTGCCACAGGTCATGATTGTGGATGAGTTTACTCTTAACTTCAAAGGGACTATTTGTATTGTATGTTGCAACTGTAAATT GAATTATTTGGCATTTTTCTCATGATTGTAATATTAATTTGAAGTTTGAATTTAATTTTCAATAAAATGGCTTTTTTGGTTTTGTT

RUNX2.F1 (forward primer)
RUNX2.R1 (reverse primer)
Single exon skipping

Frame 0

V*MLHSPHKQPQNHKCGANFLQEDSKKSLVFKW LISAGHYQPPRPTESFKAASSIYNRGYKFYLKKK GGTMASNSLFSTVTPCQQNFFWDPSTSRRFSPP SSSLQPGKMSDVSPVVAAQQQQQQQQQQQQQQQ QQQQQQQQEAAAAAAAAAAAAAAAAAVPRLRPPH DNRTMVEIIADHPAELVRTDSPNFLCSVLPSHW RCNKTLPVAFKVVALGEVPDGTVVTVMAGNDEN YSAELRNASAVMKNQVARFNDLRFVGRSGRGKSF TLTITVFTNPPQVATYHRAIKVTVDGPREPRRH RQKLDDSKPSLFSDRLSDLGRIPHPSMRVGVPP QNPRPSLNSAPSPFNPQGQSQITDPRQAQSSPPW SYDQSYPSYLSQMTSPSIHSTTPLSSTRGTGLP AITDVPRRISGASELGPFSDPRQFPSISSLTES RFSNPRMHYPATFTYTPPVTSGMSLGMSATTHYH TYLPPPYPGSSQSQSGPFQTSSTPYLYYGTSSG SYQFPMVPGGDRSPSRMLPPCTTTSNGSTLLNP NLPNQNDGVDADGSHSSSPTVLNSSGRMDESVWR PY*NSSAVAQWYLGATSHTYQYIHI*RECIYMY ID*LSTKCLFFRRFFIHSLSHDLAAIRG*ILRS RRLKRDNCNRASDCLLFKMYFYKGTKKGKGIFVF VVWSVIINNLFICQFREVDSRFRREKSKAASSL CFETSHPHGGSCVWTSALRRQLTKPVEVH*RDM R*NGCFHHSTIIQNNSSNFCFQTCCRSSSELLGS FFFFFFLLQESDFKNN*SG*IILFYFLTLLLPF SH*TKKKSHP*NLPSPFMQN*KWQYIIIAIMV* IVIAFGYVLFSFFLNYEYV*NLR*LANVNGHITL KIYL*LFNDIWTLETFLSVLIC*LRSLKVLFIK *QISSVV*SHI*NIAKQFQFIQAQCDFKKYFHL QIF*I*IVFVMYEGNVMFSSFRSLNASNTALPSK AVIRDLKNNL*PFISMKCWSDVVF*FLWGNYDS CHIKKTSTSKSLNYRKMFCGFIVKQNSKSPGFI AKT*SAKSRTCG*RVQL*DT*YRRAKLHQRFLTN QPYQTTQQGNPNLPYPRPHWQLSTEFAFRGAE* HHCPLGVGPLKRQQVPAGS*AERTYGPRGPTDS L*HLYFLQWRAKEGKGLWSLVSTSVYEFELESFC GMHFDHSWQSHGRFPSANP*SKQGSSNDTTRPI PALLPEKSGSLHWNPPPTQAEFSRDLPSNPNGP LVLWSFSNPPL*ATQHCRTACGAAGPVLPAWESP LEFILTPSNKNGWESKTLCLLKAVYQVCLDK*A TFLLLK*EGSSN*YYLLFVCGSLKHTTVHLFVS VKYVCLFQQHLKKPVSGYTFQF*LIDIKMLPPVP AASYLIKKVLYLYIIF*C*KGFFKFTVHIPSDF RDAFVLKCYYSGCRQQPRNTLEAFFPWEKFKHF FPPPSLQPPQWG*FTFLRTNSALFWSRD*NFVFL SFFFFFFH*TLNLHWVMCLICDFKTKAKSYYYC GTMY*FLGIKIAWFSL*HKHCWNLQSFQCSHIF IHFSCLTNFNPYQSSRTGTTALVLD*WNNIQPRT EKLNRLTIVCSLDGLTASHLPEPFQNEQRSPHH *GPSVINSEGRRCVYSFKDSLNSEERDWPRIQV NTWKHEALAKVIIIPMVFERTIIKDTSSKP*ICC F*KTNAFKNIFYVRIF*MCVYFMFTNNCLLFNA VL*NISAKTITYNNFLGILNKIPFLLDMLYHS* VSVEQKYL*HFV*IQAFIFIFSNCYCPRIAFHAH IVKIPLCATGHDCG*VYS*LQRDYLYCMLQL*I ELFGIFLMIVILI*SLNLIFNKMAFLVL

Frame 1

CECFIRLTNNHRTTSAVQTFSRRTARSLWFLNG *SPQVTTSHRDQQSHLRLQAVFTTEGTSSI*KK KEGLWHQTASSAQ*HHVSKTSFGIRAPAGASAPP PAACSPAK*AT*ARWWLRNSSSNSSSSNSSSSS SNSSSSSRRRRRRLRRRRRLRRRQLQCPGCGRP TTTAPWWRSSPTTRPNSSAPTAPTSCARCCPRTG AATRPCPWPSRW*PSERYQMGLWLLSWRVTMKI ILLSSGMPLLL*KTK*QGSTI*DLWAGVDEARV SP*P*PSSQILPK*LPITEQLKLQ*MDLGNPEGT DRSLMTLNLVCSLTASVI*GAFLIPV*E*VSRL RTHGPP*TLHQVLLIHKDRVRLQTPGRHSLPRR GPMTSLTPPT*AR*RPRPSTLPPRCLPHGALGFL PSPMCLGAFQVLQNWALFQTPGSSQAFHPSLRA ASPTHECTIQPPLLTPRQSPQACPSVCPPPLTT TPTCHHPTPALPKARVDPSRPAALHISTMALRQD PISFPWCRGETGLLPECFRHAPPPRMAARY*IQ ICLTRMMVLTLMEATAVPQLF*ILVAEWMNLFG DHIEIPQQWPSGIWGPHPTRINIYIYRESAYICI SISYLQSAYFLEDFSFTHSVMILQP*EGRY*EA EGSRETIAIELQIVYYLRCTFTKEQRREKVFLF LLFGLLSSITCSYANSERWTPGSGGRRAKPLPLC ALKLHTLTVAAVYGPVPSADSSQNQLRCTKGT* GRMDASITVPSFRITLPISAFRHAAGPHLNCWV RFFFFFSCSKKVTSKITDQDRLFYFTF*HSFSPF PTEPKRNPIPKTCLLLLCKTENGNTLL*P*WYR **LRLAMCCFLFF*IMNMCKI*GNLLT*MVI*L *RYIYNYLMTFGPLKHFLVY*YVDFGL*KCSLLN NKFLQWSRAISEILLSNFSSSRHNVILKNTSIS KYFRYRLFL*CMKEMLCLVLSDL*MPLTQLCLL KR*LGI*KTTFSPLSA*NAGVMWFSNFFGVIMTL VILKRQAQVNH*TTEKCSVVS*LSKTLNRQAS* QRHSQLKAAHVDRGFNYETPSTGEQNCTRDS*P TSLTKQHNRGTPICLTQGPTGSFPQNLHLEEQND ITVLWE*VL*KGSRFQQVAELRGHMAHGDLQTA FDICISYNGGPRRARGCGVWCLLVCMNLS*SPS VACTLTTPGSHMADFQVQILNPNKDHLMTPPGQS LLSSPKSQGPFIGILHPPKQNLAEICLQTLTAP LFSGPSQTHLCRPPSIAGQRVGQLDLCFLPGSL PWNSS*LLLIKMDGKAKHFAF*RPYTKYA*INKP LFYYLSKKEVVIDTIYCLCVVA*STPLSIYL*V *NMCVCFSST*KSQCLVTHFNFN*LT*KCYRQC QLHPI*LKRYYICTLFFNVKRAFLSLQYTYRVTL GMLLC*NVTIVAAGSNPETL*KLFFLGKNSSTS SLHPHSNHPNGGNSHFLEQILPFFGLGIKILFF FLSFFFFFTEPLICTGSCV*FVISRPKQSLTTTV EPCTSSWELK*RGSLCSTNIAGICSLFNAATFL SISVVSQILTHIRVPEQVPQLWF*ISGITFSPE LRNSTD*LSFAL*TVSLPLTCQSPFKMSREVHTI RDHL**IQKGGDVCTALRIPSIPRKGTGPESRL IHGNTKH*QK**LYLWYLKEQ**KTLLPNLEFV VFRKRMHLKIFSM*EFFRCVFTSCLQITVCFLMQ YFEIYQPKP*LTIIS*VF*IKFHFFWICFTILR FLWNKNICSILCKYKLSFLFFPIAIAQELLSMH IL*KFRFVPQVMIVDEFTLNFKGTICIVCCNCKL NYLAFFS*L*Y*FEV*I*FSIKWLFWFC

Frame 2

VNASFASQTTTEPQVRCKLSPGGQQEVSGF*MV NLRRSLPATETNRVI*GCKQYLQQRVQVLSEKK RRDYGIKQPLQHSDTMSAKLLLGSEHQPALQPP LQQPAARQNERREPGGGCATAAATAAAATAAAAA ATAAAAAGGGGGGCGGGGGCGGGSCSAPVAAAP RQPHHGGDHRRPPGRTRPHRQPQLPVLGAALAL ALQQDPARGLQGGSPRRGTRWDCGYCHGG*R*KL FC*APECLCCYEKPSSKVQRSEICGPEWTRQEF HLDHNRLHKSSPSSYLSQSN*SYSRWTSGTQKA QTEA**L*T*FVL*PPQ*FRAHSSSQYESRCPAS EPTALPELCTKSF*STRTESDYRPQAGTVFPAV VL*PVLPLLPEPDDVPVHPLYHPAVFHTGHWAS CHHRCA*AHFRCFRTGPFFRPQAVPKHFIPH*EP LLQPTNALSSHLYLHPASHLRHVPRYVRHHSLP HLPATTLPRLFPKPEWTLPDQQHSISLLWHFVR ILSVSHGAGGRPVSFQNASAMHHHLEWQHAIKSK FA*PE*WC*R*WKPQQFPNCFEF*WQNG*ICLA TILKFLSSGPVVSGGHIPHVSIYTYIERVHIYV YRLAIYKVPIF*KIFHSLTQS*SCSHKRVDIEKQ KAQERQLQSSFRLFTI*DVLLQRNKEGKRYFCF CCLVCYHQ*PVHMPIQRGGLQVQEGEEQSRFLS VL*NFTPSRWQLCMDQCPPQTAHKTS*GALKGHE VEWMLPSQYHHSE*LFQFLLSDMLQVLI*TVGF VFFFFFPAPRK*LQK*LIRIDYFILLFNTPSPL FPLNQKEIPSLKPAFSFYAKLKMAIHYYSHNGID SDCVWLCVVFFFFKL*ICVKSEVTC*REWSYNF KDIFIII**HLDP*NIS*CIDMLTSVSKSALY* ITNFFSGLEPYLKYC*AISVHPGTM*F*KILPSP NILDIDCFCDV*RKCYV*FFQIFECL*HSFAF* SGN*GFKKQPLALYQHEMLE*CGFLISLG*L*L LSY*KDKHK*IIELQKNVLWFHS*AKL*IARLHS KDIVS*KPHMWIEGSIMRHLVQESKIAPEILNQ PALPNNTTGEPQSALPKAPLAAFHRICI*RSRM TSLSFGSRSSEKAAGSSR*LS*EDIWPTGTYRQP LTFVFLTMEGQGGQGAVEFGVY*CV*I*ARVLL WHAL*PLLAVTWQISKCKSLIQTRII**HHQAN PCSPPRKVRVPSLESSTHPSRI*QRFAFKP*RPP CSLVLLKPTFVGHPALQDSVWGSWTCASCLGVS LGIHPDSF**KWMGKQNTLPSKGRIPSMLR*IS HFSIT*VRRK**LILFIVCVW*LEAHHCPFICKC KICVFVSAALKKASVWLHISILIN*HKNATASA SCILFN*KGTIFVHYFLMLKGLF*VYSTHTE*L *GCFCVEMLL*WLQAATQKHFRSFFSLGKIQALL PSTLTPTTPMGVIHIS*NKFCPFLV*GLKFCFS FFLFFFFSLNP*FALGHVFDL*FQDQSKVLLLL WNHVLVPGN*NSVVLFVAQTLLEFAVFSMQPHFY PFQLSHKF*PISEFQNRYHSFGFRLVE*HSAQN *ETQQINYRLLFRRSHCLSLARALSK*AEKSTP LGTICDKFRREEMCVQL*GFPQFRGKGLAQNPG* YMETRSISKSNNYTYGI*KNNNKRHFFQTLNLL FLENECI*KYFLCENFLDVCLLHVYK*LFAF*C STLKYISQNHNLQ*FLRYSE*NSISFGYALPFLG FCGTKIFVAFCVNTSFHFYFFQLLLPKNCFPCT YCKNSALCHRS*LWMSLLLTSKGLFVLYVATVN *IIWHFSHDCNINLKFEFNFQ*NGFFGFV

Sequence RUNX2.cAug05

GGACTCCAACTGGGAGGGGGCGCCAGCGCACCCAGGACGCGGTGCCCCAAGGGACCCCATTCCAAGCTGCAAACTCAAGTCCCCCGCCTCCCCAGGCCAA ACACACCCCCGCGCCCGTTCGCAGCTGCCACCCCGGGTGTTCCAAAGACTCCGGCAAAGATCTGCGCCTCCCGGGTCTCCCTACCCGCCAGCCCGACTGC GCCGCATCGCCAGACTCTGTTGGCCCATCAGACTCCGCCCGGCAGTCGGCCTCATCAAACTTGATTTCTCACCTCCTCAGCCCCATCACCTCCATCCTCT TTCCCCCCGTCTCGCCTTCACCCCCCCAATTTCCTCCTTGCCCCTCATTTCCACCCTCCTCCCCCTCCCCCGGCCACTTCGCTAACTTGTGGCTGTTGTG ATGCGTATTCCCGTAGATCCGAGCACCAGCCGGCGCTTCAGCCCCCCCTCCAGCAGCCTGCAGCCCGGCAAAATGAGCGACGTGAGCCCGGTGGTGGCTG CGCAACAGCAGCAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGGAGGCGGCGGCGGCGGCTGCGGCGGCGGC GGCGGCTGCGGCGGCGGCAGCTGCAGTGCCCCGGTTGCGGCCGCCCCACGACAACCGCACCATGGTGGAGATCATCGCCGACCACCCGGCCGAACTCGTC CGCACCGACAGCCCCAACTTCCTGTGCTCGGTGCTGCCCTCGCACTGGCGCTGCAACAAGACCCTGCCCGTGGCCTTCAAGGTGGTAGCCCTCGGAGAGG TACCAGATGGGACTGTGGTTACTGTCATGGCGGGTAACGATGAAAATTATTCTGCTGAGCTCCGGAATGCCTCTGCTGTTATGAAAAACCAAGTAGCAAG GTTCAACGATCTGAGATTTGTGGGCCGGAGTGGACGAGGCAAGAGTTTCACCTTGACCATAACCGTCTTCACAAATCCTCCCCAAGTAGCTACCTATCAC AGAGCAATTAAAGTTACAGTAGATGGACCTCGGGAACCCAGAAGGCACAGACAGAAGCTTGATGACTCTAAACCTAGTTTGTTCTCTGACCGCCTCAGTG ATTTAGGGCGCATTCCTCATCCCAGTATGAGAGTAGGTGTCCCGCCTCAGAACCCACGGCCCTCCCTGAACTCTGCACCAAGTCCTTTTAATCCACAAGG ACAGAGTCAGATTACAGACCCCAGGCAGGCACAGTCTTCCCCGCCGTGGTCCTATGACCAGTCTTACCCCTCCTACCTGAGCCAGATGACGTCCCCGTCC ATCCACTCTACCACCCCGCTGTCTTCCACACGGGGCACTGGGCTTCCTGCCATCACCGATGTGCCTAGGCGCATTTCAGATGATGACACTGCCACCTCTG ACTTCTGCCTCTGGCCTTCCACTCTCAGTAAGAAGAGCCAGGCAGGTGCTTCAGAACTGGGCCCTTTTTCAGACCCCAGGCAGTTCCCAAGCATTTCATC CCTCACTGAGAGCCGCTTCTCCAACCCACGAATGCACTATCCAGCCACCTTTACTTACACCCCGCCAGTCACCTCAGGCATGTCCCTCGGTATGTCCGCC ACCACTCACTACCACACCTACCTGCCACCACCCTACCCCGGCTCTTCCCAAAGCCAGAGTGGACCCTTCCAGACCAGCAGCACTCCATATCTCTACTATG GCACTTCGTCAGGATCCTATCAGTTTCCCATGGTGCCGGGGGGAGACCGGTCTCCTTCCAGAATGCTTCCGCCATGCACCACCACCTCGAATGGCAGCAC GCTATTAAATCCAAATTTGCCTAACCAGAATGATGGTGTTGACGCTGATGGAAGCCACAGCAGTTCCCCAACTGTTTTGAATTCTAGTGGCAGAATGGAT GAATCTGTTTGGCGACCATATTGAAATTCCTCAGCAGTGGCCCAGTGGTATCTGGGGGCCACATCCCACACGTATCAATATATACATATATAGAGAGAGT GCATATATATGTATATCGATTAGCTATCTACAAAGTGCCTATTTTTTAGAAGATTTTTCATTCACTCACTCAGTCATGATCTTGCAGCCATAAGAGGGTA GATATTGAGAAGCAGAAGGCTCAAGAGAGACAATTGCAATCGAGCTTCAGATTGTTTACTATTTAAGATGTACTTTTACAAAGGAACAAAGAAGGGAAAA GGTATTTTTGTTTTTGTTGTTTGGTCTGTTATCATCAATAACCTGTTCATATGCCAATTCAGAGAGGTGGACTCCAGGTTCAGGAGGGAGAAGAGCAAAG CCGCTTCCTCTCTGTGCTTTGAAACTTCACACCCTCACGGTGGCAGCTGTGTATGGACCAGTGCCCTCCGCAGACAGCTCACAAAACCAGTTGAGGTGCA CTAAAGGGACATGAGGTAGAATGGATGCTTCCATCACAGTACCATCATTCAGAATAACTCTTCCAATTTCTGCTTTCAGACATGCTGCAGGTCCTCATCT GAACTGTTGGGTTCGTTTTTTTTTTTTTTTTTCCTGCTCCAAGAAAGTGACTTCAAAAATAACTGATCAGGATAGATTATTTTATTTTACTTTTTAACAC TCCTTCTCCCCTTTTCCCACTGAACCAAAAAGAAATCCCATCCCTAAAACCTGCCTTCTCCTTTTATGCAAAACTGAAAATGGCAATACATTATTATAGC CATAATGGTATAGATAGTGATTGCGTTTGGCTATGTGTTGTTTTCTTTTTTTTTAAATTATGAATATGTGTAAAATCTGAGGTAACTTGCTAACGTGAAT GGTCATATAACTTTAAAGATATATTTATAATTATTTAATGACATTTGGACCCTTGAAACATTTCTTAGTGTATTGATATGTTGACTTCGGTCTCTAAAAG TGCTCTTTATTAAATAACAAATTTCTTCAGTGGTCTAGAGCCATATCTGAAATATTGCTAAGCAATTTCAGTTCATCCAGGCACAATGTGATTTTAAAAA ATACTTCCATCTCCAAATATTTTAGATATAGATTGTTTTTGTGATGTATGAAGGAAATGTTATGTTTAGTTCTTTCAGATCTTTGAATGCCTCTAACACA GCTTTGCCTTCTAAAGCGGTAATTAGGGATTTAAAAAACAACCTTTAGCCCTTTATCAGCATGAAATGCTGGAGTGATGTGGTTTTCTAATTTCTTTGGG GTAATTATGACTCTTGTCATATTAAAAAGACAAGCACAAGTAAATCATTGAACTACAGAAAAATGTTCTGTGGTTTCATAGTTAAGCAAAACTCTAAATC GCCAGGCTTCATAGCAAAGACATAGTCAGCTAAAAGCCGCACATGTGGATAGAGGGTTCAATTATGAGACACCTAGTACAGGAGAGCAAAATTGCACCAG AGATTCTTAACCAACCAGCCTTACCAAACAACACAACAGGGGAACCCCAATCTGCCTTACCCAAGGCCCCACTGGCAGCTTTCCACAGAATTTGCATTTA GAGGAGCAGAATGACATCACTGTCCTTTGGGAGTAGGTCCTCTGAAAAGGCAGCAGGTTCCAGCAGGTAGCTGAGCTGAGAGGACATATGGCCCACGGGG ACCTACAGACAGCCTTTGACATTTGTATTTCTTACAATGGAGGGCCAAGGAGGGCAAGGGGCTGTGGAGTTTGGTGTCTACTAGTGTGTATGAATTTGAG CTAGAGTCCTTCTGTGGCATGCACTTTGACCACTCCTGGCAGTCACATGGCAGATTTCCAAGTGCAAATCCTTAATCCAAACAAGGATCATCTAATGACA CCACCAGGCCAATCCCTGCTCTCCTCCCCGAAAAGTCAGGGTCCCTTCATTGGAATCCTCCACCCACCCAAGCAGAATTTAGCAGAGATTTGCCTTCAAA CCCTAACGGCCCCCTTGTTCTCTGGTCCTTCTCAAACCCACCTTTGTAGGCCACCCAGCATTGCAGGACAGCGTGTGGGGCAGCTGGACCTGTGCTTCCT GCCTGGGAGTCTCCCTTGGAATTCATCCTGACTCCTTCTAATAAAAATGGATGGGAAAGCAAAACACTTTGCCTTCTAAAGGCCGTATACCAAGTATGCT TAGATAAATAAGCCACTTTTCTATTACTTAAGTAAGAAGGAAGTAGTAATTGATACTATTTATTGTTTGTGTGTGGTAGCTTGAAGCACACCACTGTCCA TTTATTTGTAAGTGTAAAATATGTGTGTTTGTTTCAGCAGCACTTAAAAAAGCCAGTGTCTGGTTACACATTTCAATTTTAATTAATTGACATAAAAATG CTACCGCCAGTGCCAGCTGCATCCTATTTAATTAAAAAGGTACTATATTTGTACATTATTTTTTAATGTTAAAAGGGCTTTTTTAAGTTTACAGTACACA TACCGAGTGACTTTAGGGATGCTTTTGTGTTGAAATGTTACTATAGTGGCTGCAGGCAGCAACCCAGAAACACTTTAGAAGCTTTTTTTCCTTGGGAAAA ATTCAAGCACTTCTTCCCTCCACCCTCACTCCAACCACCCCAATGGGGGTAATTCACATTTCTTAGAACAAATTCTGCCCTTTTTTGGTCTAGGGATTAA AATTTTGTTTTTCTTTCTTTCTTTTTTTTTTTTTTTCACTGAACCCTTAATTTGCACTGGGTCATGTGTTTGATTTGTGATTTCAAGACCAAAGCAAAGT CTTACTACTACTGTGGAACCATGTACTAGTTCCTGGGAATTAAAATAGCGTGGTTCTCTTTGTAGCACAAACATTGCTGGAATTTGCAGTCTTTTCAATG CAGCCACATTTTTATCCATTTCAGTTGTCTCACAAATTTTAACCCATATCAGAGTTCCAGAACAGGTACCACAGCTTTGGTTTTAGATTAGTGGAATAAC ATTCAGCCCAGAACTGAGAAACTCAACAGATTAACTATCGTTTGCTCTTTAGACGGTCTCACTGCCTCTCACTTGCCAGAGCCCTTTCAAAATGAGCAGA GAAGTCCACACCATTAGGGACCATCTGTGATAAATTCAGAAGGGAGGAGATGTGTGTACAGCTTTAAGGATTCCCTCAATTCCGAGGAAAGGGACTGGCC CAGAATCCAGGTTAATACATGGAAACACGAAGCATTAGCAAAAGTAATAATTATACCTATGGTATTTGAAAGAACAATAATAAAAGACACTTCTTCCAAA CCTTGAATTTGTTGTTTTTAGAAAACGAATGCATTTAAAAATATTTTCTATGTGAGAATTTTTTAGATGTGTGTTTACTTCATGTTTACAAATAACTGTT TGCTTTTTAATGCAGTACTTTGAAATATATCAGCCAAAACCATAACTTACAATAATTTCTTAGGTATTCTGAATAAAATTCCATTTCTTTTGGATATGCT TTACCATTCTTAGGTTTCTGTGGAACAAAAATATTTGTAGCATTTTGTGTAAATACAAGCTTTCATTTTTATTTTTTCCAATTGCTATTGCCCAAGAATT GCTTTCCATGCACATATTGTAAAAATTCCGCTTTGTGCCACAGGTCATGATTGTGGATGAGTTTACTCTTAACTTCAAAGGGACTATTTGTATTGTATGT TGCAACTGTAAATTGAATTATTTGGCATTTTTCTCATGATTGTAATATTAATTTGAAGTTTGAATTTAATTTTCAATAAAATGGCTTTTTTGGTTTTGTT

RUNX2.F1 (forward primer)
RUNX2.R1 (reverse primer)
Single exon skipping

Frame 0

GLQLGGGASAPRTRCPKGPHSKLQTQVPRLPRP NTPPRPFAAATPGVPKTPAKICASRVSLPASPTA PHRQTLLAHQTPPGSRPHQT*FLTSSAPSPPSS FPPSRLHPPNFLLAPHFHPPPPPPATSLTCGCC DAYSRRSEHQPALQPPLQQPAARQNERREPGGGC ATAAATAAAATAAAAAATAAAAAGGGGGGCGGG GGCGGGSCSAPVAAAPRQPHHGGDHRRPPGRTR PHRQPQLPVLGAALALALQQDPARGLQGGSPRRG TRWDCGYCHGG*R*KLFC*APECLCCYEKPSSK VQRSEICGPEWTRQEFHLDHNRLHKSSPSSYLS QSN*SYSRWTSGTQKAQTEA**L*T*FVL*PPQ* FRAHSSSQYESRCPASEPTALPELCTKSF*STR TESDYRPQAGTVFPAVVL*PVLPLLPEPDDVPV HPLYHPAVFHTGHWASCHHRCA*AHFR**HCHL* LLPLAFHSQ*EEPGRCFRTGPFFRPQAVPKHFI PH*EPLLQPTNALSSHLYLHPASHLRHVPRYVR HHSLPHLPATTLPRLFPKPEWTLPDQQHSISLLW HFVRILSVSHGAGGRPVSFQNASAMHHHLEWQH AIKSKFA*PE*WC*R*WKPQQFPNCFEF*WQNG *ICLATILKFLSSGPVVSGGHIPHVSIYTYIERV HIYVYRLAIYKVPIF*KIFHSLTQS*SCSHKRV DIEKQKAQERQLQSSFRLFTI*DVLLQRNKEGK RYFCFCCLVCYHQ*PVHMPIQRGGLQVQEGEEQS RFLSVL*NFTPSRWQLCMDQCPPQTAHKTS*GA LKGHEVEWMLPSQYHHSE*LFQFLLSDMLQVLI *TVGFVFFFFFPAPRK*LQK*LIRIDYFILLFNT PSPLFPLNQKEIPSLKPAFSFYAKLKMAIHYYS HNGIDSDCVWLCVVFFFFKL*ICVKSEVTC*RE WSYNFKDIFIII**HLDP*NIS*CIDMLTSVSKS ALY*ITNFFSGLEPYLKYC*AISVHPGTM*F*K ILPSPNILDIDCFCDV*RKCYV*FFQIFECL*H SFAF*SGN*GFKKQPLALYQHEMLE*CGFLISLG *L*LLSY*KDKHK*IIELQKNVLWFHS*AKL*I ARLHSKDIVS*KPHMWIEGSIMRHLVQESKIAP EILNQPALPNNTTGEPQSALPKAPLAAFHRICI* RSRMTSLSFGSRSSEKAAGSSR*LS*EDIWPTG TYRQPLTFVFLTMEGQGGQGAVEFGVY*CV*I* ARVLLWHAL*PLLAVTWQISKCKSLIQTRII**H HQANPCSPPRKVRVPSLESSTHPSRI*QRFAFK P*RPPCSLVLLKPTFVGHPALQDSVWGSWTCAS CLGVSLGIHPDSF**KWMGKQNTLPSKGRIPSML R*ISHFSIT*VRRK**LILFIVCVW*LEAHHCP FICKCKICVFVSAALKKASVWLHISILIN*HKN ATASASCILFN*KGTIFVHYFLMLKGLF*VYSTH TE*L*GCFCVEMLL*WLQAATQKHFRSFFSLGK IQALLPSTLTPTTPMGVIHIS*NKFCPFLV*GL KFCFSFFLFFFFSLNP*FALGHVFDL*FQDQSKV LLLLWNHVLVPGN*NSVVLFVAQTLLEFAVFSM QPHFYPFQLSHKF*PISEFQNRYHSFGFRLVE* HSAQN*ETQQINYRLLFRRSHCLSLARALSK*AE KSTPLGTICDKFRREEMCVQL*GFPQFRGKGLA QNPG*YMETRSISKSNNYTYGI*KNNNKRHFFQ TLNLLFLENECI*KYFLCENFLDVCLLHVYK*LF AF*CSTLKYISQNHNLQ*FLRYSE*NSISFGYA LPFLGFCGTKIFVAFCVNTSFHFYFFQLLLPKN CFPCTYCKNSALCHRS*LWMSLLLTSKGLFVLYV ATVN*IIWHFSHDCNINLKFEFNFQ*NGFFGFV

Frame 1

DSNWEGAPAHPGRGAPRDPIPSCKLKSPASPGQ THPRARSQLPPRVFQRLRQRSAPPGSPYPPARL RRIARLCWPIRLRPAVGLIKLDFSPPQPHHLHPL SPRLAFTPPISSLPLISTLLPLPRPLR*LVAVV MRIPVDPSTSRRFSPPSSSLQPGKMSDVSPVVA AQQQQQQQQQQQQQQQQQQQQQQQEAAAAAAAAA AAAAAAAAVPRLRPPHDNRTMVEIIADHPAELV RTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGE VPDGTVVTVMAGNDENYSAELRNASAVMKNQVAR FNDLRFVGRSGRGKSFTLTITVFTNPPQVATYH RAIKVTVDGPREPRRHRQKLDDSKPSLFSDRLS DLGRIPHPSMRVGVPPQNPRPSLNSAPSPFNPQG QSQITDPRQAQSSPPWSYDQSYPSYLSQMTSPS IHSTTPLSSTRGTGLPAITDVPRRISDDDTATS DFCLWPSTLSKKSQAGASELGPFSDPRQFPSISS LTESRFSNPRMHYPATFTYTPPVTSGMSLGMSA TTHYHTYLPPPYPGSSQSQSGPFQTSSTPYLYY GTSSGSYQFPMVPGGDRSPSRMLPPCTTTSNGST LLNPNLPNQNDGVDADGSHSSSPTVLNSSGRMD ESVWRPY*NSSAVAQWYLGATSHTYQYIHI*RE CIYMYID*LSTKCLFFRRFFIHSLSHDLAAIRG* ILRSRRLKRDNCNRASDCLLFKMYFYKGTKKGK GIFVFVVWSVIINNLFICQFREVDSRFRREKSK AASSLCFETSHPHGGSCVWTSALRRQLTKPVEVH *RDMR*NGCFHHSTIIQNNSSNFCFQTCCRSSS ELLGSFFFFFFLLQESDFKNN*SG*IILFYFLT LLLPFSH*TKKKSHP*NLPSPFMQN*KWQYIIIA IMV*IVIAFGYVLFSFFLNYEYV*NLR*LANVN GHITLKIYL*LFNDIWTLETFLSVLIC*LRSLK VLFIK*QISSVV*SHI*NIAKQFQFIQAQCDFKK YFHLQIF*I*IVFVMYEGNVMFSSFRSLNASNT ALPSKAVIRDLKNNL*PFISMKCWSDVVF*FLW GNYDSCHIKKTSTSKSLNYRKMFCGFIVKQNSKS PGFIAKT*SAKSRTCG*RVQL*DT*YRRAKLHQ RFLTNQPYQTTQQGNPNLPYPRPHWQLSTEFAF RGAE*HHCPLGVGPLKRQQVPAGS*AERTYGPRG PTDSL*HLYFLQWRAKEGKGLWSLVSTSVYEFE LESFCGMHFDHSWQSHGRFPSANP*SKQGSSND TTRPIPALLPEKSGSLHWNPPPTQAEFSRDLPSN PNGPLVLWSFSNPPL*ATQHCRTACGAAGPVLP AWESPLEFILTPSNKNGWESKTLCLLKAVYQVC LDK*ATFLLLK*EGSSN*YYLLFVCGSLKHTTVH LFVSVKYVCLFQQHLKKPVSGYTFQF*LIDIKM LPPVPAASYLIKKVLYLYIIF*C*KGFFKFTVH IPSDFRDAFVLKCYYSGCRQQPRNTLEAFFPWEK FKHFFPPPSLQPPQWG*FTFLRTNSALFWSRD* NFVFLSFFFFFFH*TLNLHWVMCLICDFKTKAK SYYYCGTMY*FLGIKIAWFSL*HKHCWNLQSFQC SHIFIHFSCLTNFNPYQSSRTGTTALVLD*WNN IQPRTEKLNRLTIVCSLDGLTASHLPEPFQNEQ RSPHH*GPSVINSEGRRCVYSFKDSLNSEERDWP RIQVNTWKHEALAKVIIIPMVFERTIIKDTSSK P*ICCF*KTNAFKNIFYVRIF*MCVYFMFTNNC LLFNAVL*NISAKTITYNNFLGILNKIPFLLDML YHS*VSVEQKYL*HFV*IQAFIFIFSNCYCPRI AFHAHIVKIPLCATGHDCG*VYS*LQRDYLYCM LQL*IELFGIFLMIVILI*SLNLIFNKMAFLVL

Frame 2

TPTGRGRQRTQDAVPQGTPFQAANSSPPPPQAK HTPAPVRSCHPGCSKDSGKDLRLPGLPTRQPDC AASPDSVGPSDSARQSASSNLISHLLSPITSIL FPPVSPSPPQFPPCPSFPPSSPSPGHFANLWLL* CVFP*IRAPAGASAPPPAACSPAK*AT*ARWWL RNSSSNSSSSNSSSSSSNSSSSSRRRRRRLRRR RRLRRRQLQCPGCGRPTTTAPWWRSSPTTRPNSS APTAPTSCARCCPRTGAATRPCPWPSRW*PSER YQMGLWLLSWRVTMKIILLSSGMPLLL*KTK*Q GSTI*DLWAGVDEARVSP*P*PSSQILPK*LPIT EQLKLQ*MDLGNPEGTDRSLMTLNLVCSLTASV I*GAFLIPV*E*VSRLRTHGPP*TLHQVLLIHK DRVRLQTPGRHSLPRRGPMTSLTPPT*AR*RPRP STLPPRCLPHGALGFLPSPMCLGAFQMMTLPPL TSASGLPLSVRRARQVLQNWALFQTPGSSQAFH PSLRAASPTHECTIQPPLLTPRQSPQACPSVCPP PLTTTPTCHHPTPALPKARVDPSRPAALHISTM ALRQDPISFPWCRGETGLLPECFRHAPPPRMAA RY*IQICLTRMMVLTLMEATAVPQLF*ILVAEWM NLFGDHIEIPQQWPSGIWGPHPTRINIYIYRES AYICISISYLQSAYFLEDFSFTHSVMILQP*EG RY*EAEGSRETIAIELQIVYYLRCTFTKEQRREK VFLFLLFGLLSSITCSYANSERWTPGSGGRRAK PLPLCALKLHTLTVAAVYGPVPSADSSQNQLRC TKGT*GRMDASITVPSFRITLPISAFRHAAGPHL NCWVRFFFFFSCSKKVTSKITDQDRLFYFTF*H SFSPFPTEPKRNPIPKTCLLLLCKTENGNTLL* P*WYR**LRLAMCCFLFF*IMNMCKI*GNLLT*M VI*L*RYIYNYLMTFGPLKHFLVY*YVDFGL*K CSLLNNKFLQWSRAISEILLSNFSSSRHNVILK NTSISKYFRYRLFL*CMKEMLCLVLSDL*MPLTQ LCLLKR*LGI*KTTFSPLSA*NAGVMWFSNFFG VIMTLVILKRQAQVNH*TTEKCSVVS*LSKTLN RQAS*QRHSQLKAAHVDRGFNYETPSTGEQNCTR DS*PTSLTKQHNRGTPICLTQGPTGSFPQNLHL EEQNDITVLWE*VL*KGSRFQQVAELRGHMAHG DLQTAFDICISYNGGPRRARGCGVWCLLVCMNLS *SPSVACTLTTPGSHMADFQVQILNPNKDHLMT PPGQSLLSSPKSQGPFIGILHPPKQNLAEICLQ TLTAPLFSGPSQTHLCRPPSIAGQRVGQLDLCFL PGSLPWNSS*LLLIKMDGKAKHFAF*RPYTKYA *INKPLFYYLSKKEVVIDTIYCLCVVA*STPLS IYL*V*NMCVCFSST*KSQCLVTHFNFN*LT*KC YRQCQLHPI*LKRYYICTLFFNVKRAFLSLQYT YRVTLGMLLC*NVTIVAAGSNPETL*KLFFLGK NSSTSSLHPHSNHPNGGNSHFLEQILPFFGLGIK ILFFFLSFFFFFTEPLICTGSCV*FVISRPKQS LTTTVEPCTSSWELK*RGSLCSTNIAGICSLFN AATFLSISVVSQILTHIRVPEQVPQLWF*ISGIT FSPELRNSTD*LSFAL*TVSLPLTCQSPFKMSR EVHTIRDHL**IQKGGDVCTALRIPSIPRKGTG PESRLIHGNTKH*QK**LYLWYLKEQ**KTLLPN LEFVVFRKRMHLKIFSM*EFFRCVFTSCLQITV CFLMQYFEIYQPKP*LTIIS*VF*IKFHFFWIC FTILRFLWNKNICSILCKYKLSFLFFPIAIAQEL LSMHIL*KFRFVPQVMIVDEFTLNFKGTICIVC CNCKLNYLAFFS*L*Y*FEV*I*FSIKWLFWFC

Sequence RUNX2.aAug05

GTGTGAATGCTTCATTCGCCTCACAAACAACCACAGAACCACAAGTGCGGTGCAAACTTTCTCCAGGAGGACAGCAAGAAGTCTCTGGTTTTTAAATGGT TAATCTCCGCAGGTCACTACCAGCCACCGAGACCAACAGAGTCATTTAAGGCTGCAAGCAGTATTTACAACAGAGGGTACAAGTTCTATCTGAAAAAAAA AGGAGGGACTATGGCATCAAACAGCCTCTTCAGCACAGTGACACCATGTCAGCAAAACTTCTTTTGGGATCCGAGCACCAGCCGGCGCTTCAGCCCCCCC TCCAGCAGCCTGCAGCCCGGCAAAATGAGCGACGTGAGCCCGGTGGTGGCTGCGCAACAGCAGCAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGCAGC AGCAACAGCAGCAGCAGCAGCAGGAGGCGGCGGCGGCGGCTGCGGCGGCGGCGGCGGCTGCGGCGGCGGCAGCTGCAGTGCCCCGGTTGCGGCCGCCCCA CGACAACCGCACCATGGTGGAGATCATCGCCGACCACCCGGCCGAACTCGTCCGCACCGACAGCCCCAACTTCCTGTGCTCGGTGCTGCCCTCGCACTGG CGCTGCAACAAGACCCTGCCCGTGGCCTTCAAGGTGGTAGCCCTCGGAGAGGTACCAGATGGGACTGTGGTTACTGTCATGGCGGGTAACGATGAAAATT ATTCTGCTGAGCTCCGGAATGCCTCTGCTGTTATGAAAAACCAAGTAGCAAGGTTCAACGATCTGAGATTTGTGGGCCGGAGTGGACGAGGCAAGAGTTT CACCTTGACCATAACCGTCTTCACAAATCCTCCCCAAGTAGCTACCTATCACAGAGCAATTAAAGTTACAGTAGATGGACCTCGGGAACCCAGAAGGCAC AGACAGAAGCTTGATGACTCTAAACCTAGTTTGTTCTCTGACCGCCTCAGTGATTTAGGGCGCATTCCTCATCCCAGTATGAGAGTAGGTGTCCCGCCTC AGAACCCACGGCCCTCCCTGAACTCTGCACCAAGTCCTTTTAATCCACAAGGACAGAGTCAGATTACAGACCCCAGGCAGGCACAGTCTTCCCCGCCGTG GTCCTATGACCAGTCTTACCCCTCCTACCTGAGCCAGATGACGTCCCCGTCCATCCACTCTACCACCCCGCTGTCTTCCACACGGGGCACTGGGCTTCCT GCCATCACCGATGTGCCTAGGCGCATTTCAGATGATGACACTGCCACCTCTGACTTCTGCCTCTGGCCTTCCACTCTCAGTAAGAAGAGCCAGGCAGGTG CTTCAGAACTGGGCCCTTTTTCAGACCCCAGGCAGTTCCCAAGCATTTCATCCCTCACTGAGAGCCGCTTCTCCAACCCACGAATGCACTATCCAGCCAC CTTTACTTACACCCCGCCAGTCACCTCAGGCATGTCCCTCGGTATGTCCGCCACCACTCACTACCACACCTACCTGCCACCACCCTACCCCGGCTCTTCC CAAAGCCAGAGTGGACCCTTCCAGACCAGCAGCACTCCATATCTCTACTATGGCACTTCGTCAGGATCCTATCAGTTTCCCATGGTGCCGGGGGGAGACC GGTCTCCTTCCAGAATGCTTCCGCCATGCACCACCACCTCGAATGGCAGCACGCTATTAAATCCAAATTTGCCTAACCAGAATGATGGTGTTGACGCTGA TGGAAGCCACAGCAGTTCCCCAACTGTTTTGAATTCTAGTGGCAGAATGGATGAATCTGTTTGGCGACCATATTGAAATTCCTCAGCAGTGGCCCAGTGG TATCTGGGGGCCACATCCCACACGTATCAATATATACATATATAGAGAGAGTGCATATATATGTATATCGATTAGCTATCTACAAAGTGCCTATTTTTTA GAAGATTTTTCATTCACTCACTCAGTCATGATCTTGCAGCCATAAGAGGGTAGATATTGAGAAGCAGAAGGCTCAAGAGAGACAATTGCAATCGAGCTTC AGATTGTTTACTATTTAAGATGTACTTTTACAAAGGAACAAAGAAGGGAAAAGGTATTTTTGTTTTTGTTGTTTGGTCTGTTATCATCAATAACCTGTTC ATATGCCAATTCAGAGAGGTGGACTCCAGGTTCAGGAGGGAGAAGAGCAAAGCCGCTTCCTCTCTGTGCTTTGAAACTTCACACCCTCACGGTGGCAGCT GTGTATGGACCAGTGCCCTCCGCAGACAGCTCACAAAACCAGTTGAGGTGCACTAAAGGGACATGAGGTAGAATGGATGCTTCCATCACAGTACCATCAT TCAGAATAACTCTTCCAATTTCTGCTTTCAGACATGCTGCAGGTCCTCATCTGAACTGTTGGGTTCGTTTTTTTTTTTTTTTTTCCTGCTCCAAGAAAGT GACTTCAAAAATAACTGATCAGGATAGATTATTTTATTTTACTTTTTAACACTCCTTCTCCCCTTTTCCCACTGAACCAAAAAGAAATCCCATCCCTAAA ACCTGCCTTCTCCTTTTATGCAAAACTGAAAATGGCAATACATTATTATAGCCATAATGGTATAGATAGTGATTGCGTTTGGCTATGTGTTGTTTTCTTT TTTTTTAAATTATGAATATGTGTAAAATCTGAGGTAACTTGCTAACGTGAATGGTCATATAACTTTAAAGATATATTTATAATTATTTAATGACATTTGG ACCCTTGAAACATTTCTTAGTGTATTGATATGTTGACTTCGGTCTCTAAAAGTGCTCTTTATTAAATAACAAATTTCTTCAGTGGTCTAGAGCCATATCT GAAATATTGCTAAGCAATTTCAGTTCATCCAGGCACAATGTGATTTTAAAAAATACTTCCATCTCCAAATATTTTAGATATAGATTGTTTTTGTGATGTA TGAAGGAAATGTTATGTTTAGTTCTTTCAGATCTTTGAATGCCTCTAACACAGCTTTGCCTTCTAAAGCGGTAATTAGGGATTTAAAAAACAACCTTTAG CCCTTTATCAGCATGAAATGCTGGAGTGATGTGGTTTTCTAATTTCTTTGGGGTAATTATGACTCTTGTCATATTAAAAAGACAAGCACAAGTAAATCAT TGAACTACAGAAAAATGTTCTGTGGTTTCATAGTTAAGCAAAACTCTAAATCGCCAGGCTTCATAGCAAAGACATAGTCAGCTAAAAGCCGCACATGTGG ATAGAGGGTTCAATTATGAGACACCTAGTACAGGAGAGCAAAATTGCACCAGAGATTCTTAACCAACCAGCCTTACCAAACAACACAACAGGGGAACCCC AATCTGCCTTACCCAAGGCCCCACTGGCAGCTTTCCACAGAATTTGCATTTAGAGGAGCAGAATGACATCACTGTCCTTTGGGAGTAGGTCCTCTGAAAA GGCAGCAGGTTCCAGCAGGTAGCTGAGCTGAGAGGACATATGGCCCACGGGGACCTACAGACAGCCTTTGACATTTGTATTTCTTACAATGGAGGGCCAA GGAGGGCAAGGGGCTGTGGAGTTTGGTGTCTACTAGTGTGTATGAATTTGAGCTAGAGTCCTTCTGTGGCATGCACTTTGACCACTCCTGGCAGTCACAT GGCAGATTTCCAAGTGCAAATCCTTAATCCAAACAAGGATCATCTAATGACACCACCAGGCCAATCCCTGCTCTCCTCCCCGAAAAGTCAGGGTCCCTTC ATTGGAATCCTCCACCCACCCAAGCAGAATTTAGCAGAGATTTGCCTTCAAACCCTAACGGCCCCCTTGTTCTCTGGTCCTTCTCAAACCCACCTTTGTA GGCCACCCAGCATTGCAGGACAGCGTGTGGGGCAGCTGGACCTGTGCTTCCTGCCTGGGAGTCTCCCTTGGAATTCATCCTGACTCCTTCTAATAAAAAT GGATGGGAAAGCAAAACACTTTGCCTTCTAAAGGCCGTATACCAAGTATGCTTAGATAAATAAGCCACTTTTCTATTACTTAAGTAAGAAGGAAGTAGTA ATTGATACTATTTATTGTTTGTGTGTGGTAGCTTGAAGCACACCACTGTCCATTTATTTGTAAGTGTAAAATATGTGTGTTTGTTTCAGCAGCACTTAAA AAAGCCAGTGTCTGGTTACACATTTCAATTTTAATTAATTGACATAAAAATGCTACCGCCAGTGCCAGCTGCATCCTATTTAATTAAAAAGGTACTATAT TTGTACATTATTTTTTAATGTTAAAAGGGCTTTTTTAAGTTTACAGTACACATACCGAGTGACTTTAGGGATGCTTTTGTGTTGAAATGTTACTATAGTG GCTGCAGGCAGCAACCCAGAAACACTTTAGAAGCTTTTTTTCCTTGGGAAAAATTCAAGCACTTCTTCCCTCCACCCTCACTCCAACCACCCCAATGGGG GTAATTCACATTTCTTAGAACAAATTCTGCCCTTTTTTGGTCTAGGGATTAAAATTTTGTTTTTCTTTCTTTCTTTTTTTTTTTTTTTCACTGAACCCTT AATTTGCACTGGGTCATGTGTTTGATTTGTGATTTCAAGACCAAAGCAAAGTCTTACTACTACTGTGGAACCATGTACTAGTTCCTGGGAATTAAAATAG CGTGGTTCTCTTTGTAGCACAAACATTGCTGGAATTTGCAGTCTTTTCAATGCAGCCACATTTTTATCCATTTCAGTTGTCTCACAAATTTTAACCCATA TCAGAGTTCCAGAACAGGTACCACAGCTTTGGTTTTAGATTAGTGGAATAACATTCAGCCCAGAACTGAGAAACTCAACAGATTAACTATCGTTTGCTCT TTAGACGGTCTCACTGCCTCTCACTTGCCAGAGCCCTTTCAAAATGAGCAGAGAAGTCCACACCATTAGGGACCATCTGTGATAAATTCAGAAGGGAGGA GATGTGTGTACAGCTTTAAGGATTCCCTCAATTCCGAGGAAAGGGACTGGCCCAGAATCCAGGTTAATACATGGAAACACGAAGCATTAGCAAAAGTAAT AATTATACCTATGGTATTTGAAAGAACAATAATAAAAGACACTTCTTCCAAACCTTGAATTTGTTGTTTTTAGAAAACGAATGCATTTAAAAATATTTTC TATGTGAGAATTTTTTAGATGTGTGTTTACTTCATGTTTACAAATAACTGTTTGCTTTTTAATGCAGTACTTTGAAATATATCAGCCAAAACCATAACTT ACAATAATTTCTTAGGTATTCTGAATAAAATTCCATTTCTTTTGGATATGCTTTACCATTCTTAGGTTTCTGTGGAACAAAAATATTTGTAGCATTTTGT GTAAATACAAGCTTTCATTTTTATTTTTTCCAATTGCTATTGCCCAAGAATTGCTTTCCATGCACATATTGTAAAAATTCCGCTTTGTGCCACAGGTCAT GATTGTGGATGAGTTTACTCTTAACTTCAAAGGGACTATTTGTATTGTATGTTGCAACTGTAAATTGAATTATTTGGCATTTTTCTCATGATTGTAATAT TAATTTGAAGTTTGAATTTAATTTTCAATAAAATGGCTTTTTTGGTTTTGTT

RUNX2.F1 (forward primer)
RUNX2.R1 (reverse primer)
Single exon skipping

Frame 0

V*MLHSPHKQPQNHKCGANFLQEDSKKSLVFKW LISAGHYQPPRPTESFKAASSIYNRGYKFYLKKK GGTMASNSLFSTVTPCQQNFFWDPSTSRRFSPP SSSLQPGKMSDVSPVVAAQQQQQQQQQQQQQQQ QQQQQQQQEAAAAAAAAAAAAAAAAAVPRLRPPH DNRTMVEIIADHPAELVRTDSPNFLCSVLPSHW RCNKTLPVAFKVVALGEVPDGTVVTVMAGNDEN YSAELRNASAVMKNQVARFNDLRFVGRSGRGKSF TLTITVFTNPPQVATYHRAIKVTVDGPREPRRH RQKLDDSKPSLFSDRLSDLGRIPHPSMRVGVPP QNPRPSLNSAPSPFNPQGQSQITDPRQAQSSPPW SYDQSYPSYLSQMTSPSIHSTTPLSSTRGTGLP AITDVPRRISDDDTATSDFCLWPSTLSKKSQAG ASELGPFSDPRQFPSISSLTESRFSNPRMHYPAT FTYTPPVTSGMSLGMSATTHYHTYLPPPYPGSS QSQSGPFQTSSTPYLYYGTSSGSYQFPMVPGGD RSPSRMLPPCTTTSNGSTLLNPNLPNQNDGVDAD GSHSSSPTVLNSSGRMDESVWRPY*NSSAVAQW YLGATSHTYQYIHI*RECIYMYID*LSTKCLFF RRFFIHSLSHDLAAIRG*ILRSRRLKRDNCNRAS DCLLFKMYFYKGTKKGKGIFVFVVWSVIINNLF ICQFREVDSRFRREKSKAASSLCFETSHPHGGS CVWTSALRRQLTKPVEVH*RDMR*NGCFHHSTII QNNSSNFCFQTCCRSSSELLGSFFFFFFLLQES DFKNN*SG*IILFYFLTLLLPFSH*TKKKSHP* NLPSPFMQN*KWQYIIIAIMV*IVIAFGYVLFSF FLNYEYV*NLR*LANVNGHITLKIYL*LFNDIW TLETFLSVLIC*LRSLKVLFIK*QISSVV*SHI *NIAKQFQFIQAQCDFKKYFHLQIF*I*IVFVMY EGNVMFSSFRSLNASNTALPSKAVIRDLKNNL* PFISMKCWSDVVF*FLWGNYDSCHIKKTSTSKS LNYRKMFCGFIVKQNSKSPGFIAKT*SAKSRTCG *RVQL*DT*YRRAKLHQRFLTNQPYQTTQQGNP NLPYPRPHWQLSTEFAFRGAE*HHCPLGVGPLK RQQVPAGS*AERTYGPRGPTDSL*HLYFLQWRAK EGKGLWSLVSTSVYEFELESFCGMHFDHSWQSH GRFPSANP*SKQGSSNDTTRPIPALLPEKSGSL HWNPPPTQAEFSRDLPSNPNGPLVLWSFSNPPL* ATQHCRTACGAAGPVLPAWESPLEFILTPSNKN GWESKTLCLLKAVYQVCLDK*ATFLLLK*EGSS N*YYLLFVCGSLKHTTVHLFVSVKYVCLFQQHLK KPVSGYTFQF*LIDIKMLPPVPAASYLIKKVLY LYIIF*C*KGFFKFTVHIPSDFRDAFVLKCYYS GCRQQPRNTLEAFFPWEKFKHFFPPPSLQPPQWG *FTFLRTNSALFWSRD*NFVFLSFFFFFFH*TL NLHWVMCLICDFKTKAKSYYYCGTMY*FLGIKI AWFSL*HKHCWNLQSFQCSHIFIHFSCLTNFNPY QSSRTGTTALVLD*WNNIQPRTEKLNRLTIVCS LDGLTASHLPEPFQNEQRSPHH*GPSVINSEGR RCVYSFKDSLNSEERDWPRIQVNTWKHEALAKVI IIPMVFERTIIKDTSSKP*ICCF*KTNAFKNIF YVRIF*MCVYFMFTNNCLLFNAVL*NISAKTIT YNNFLGILNKIPFLLDMLYHS*VSVEQKYL*HFV *IQAFIFIFSNCYCPRIAFHAHIVKIPLCATGH DCG*VYS*LQRDYLYCMLQL*IELFGIFLMIVI LI*SLNLIFNKMAFLVL

Frame 1

CECFIRLTNNHRTTSAVQTFSRRTARSLWFLNG *SPQVTTSHRDQQSHLRLQAVFTTEGTSSI*KK KEGLWHQTASSAQ*HHVSKTSFGIRAPAGASAPP PAACSPAK*AT*ARWWLRNSSSNSSSSNSSSSS SNSSSSSRRRRRRLRRRRRLRRRQLQCPGCGRP TTTAPWWRSSPTTRPNSSAPTAPTSCARCCPRTG AATRPCPWPSRW*PSERYQMGLWLLSWRVTMKI ILLSSGMPLLL*KTK*QGSTI*DLWAGVDEARV SP*P*PSSQILPK*LPITEQLKLQ*MDLGNPEGT DRSLMTLNLVCSLTASVI*GAFLIPV*E*VSRL RTHGPP*TLHQVLLIHKDRVRLQTPGRHSLPRR GPMTSLTPPT*AR*RPRPSTLPPRCLPHGALGFL PSPMCLGAFQMMTLPPLTSASGLPLSVRRARQV LQNWALFQTPGSSQAFHPSLRAASPTHECTIQP PLLTPRQSPQACPSVCPPPLTTTPTCHHPTPALP KARVDPSRPAALHISTMALRQDPISFPWCRGET GLLPECFRHAPPPRMAARY*IQICLTRMMVLTL MEATAVPQLF*ILVAEWMNLFGDHIEIPQQWPSG IWGPHPTRINIYIYRESAYICISISYLQSAYFL EDFSFTHSVMILQP*EGRY*EAEGSRETIAIEL QIVYYLRCTFTKEQRREKVFLFLLFGLLSSITCS YANSERWTPGSGGRRAKPLPLCALKLHTLTVAA VYGPVPSADSSQNQLRCTKGT*GRMDASITVPS FRITLPISAFRHAAGPHLNCWVRFFFFFSCSKKV TSKITDQDRLFYFTF*HSFSPFPTEPKRNPIPK TCLLLLCKTENGNTLL*P*WYR**LRLAMCCFL FF*IMNMCKI*GNLLT*MVI*L*RYIYNYLMTFG PLKHFLVY*YVDFGL*KCSLLNNKFLQWSRAIS EILLSNFSSSRHNVILKNTSISKYFRYRLFL*C MKEMLCLVLSDL*MPLTQLCLLKR*LGI*KTTFS PLSA*NAGVMWFSNFFGVIMTLVILKRQAQVNH *TTEKCSVVS*LSKTLNRQAS*QRHSQLKAAHV DRGFNYETPSTGEQNCTRDS*PTSLTKQHNRGTP ICLTQGPTGSFPQNLHLEEQNDITVLWE*VL*K GSRFQQVAELRGHMAHGDLQTAFDICISYNGGP RRARGCGVWCLLVCMNLS*SPSVACTLTTPGSHM ADFQVQILNPNKDHLMTPPGQSLLSSPKSQGPF IGILHPPKQNLAEICLQTLTAPLFSGPSQTHLC RPPSIAGQRVGQLDLCFLPGSLPWNSS*LLLIKM DGKAKHFAF*RPYTKYA*INKPLFYYLSKKEVV IDTIYCLCVVA*STPLSIYL*V*NMCVCFSST* KSQCLVTHFNFN*LT*KCYRQCQLHPI*LKRYYI CTLFFNVKRAFLSLQYTYRVTLGMLLC*NVTIV AAGSNPETL*KLFFLGKNSSTSSLHPHSNHPNG GNSHFLEQILPFFGLGIKILFFFLSFFFFFTEPL ICTGSCV*FVISRPKQSLTTTVEPCTSSWELK* RGSLCSTNIAGICSLFNAATFLSISVVSQILTH IRVPEQVPQLWF*ISGITFSPELRNSTD*LSFAL *TVSLPLTCQSPFKMSREVHTIRDHL**IQKGG DVCTALRIPSIPRKGTGPESRLIHGNTKH*QK* *LYLWYLKEQ**KTLLPNLEFVVFRKRMHLKIFS M*EFFRCVFTSCLQITVCFLMQYFEIYQPKP*L TIIS*VF*IKFHFFWICFTILRFLWNKNICSIL CKYKLSFLFFPIAIAQELLSMHIL*KFRFVPQVM IVDEFTLNFKGTICIVCCNCKLNYLAFFS*L*Y *FEV*I*FSIKWLFWFC

Frame 2

VNASFASQTTTEPQVRCKLSPGGQQEVSGF*MV NLRRSLPATETNRVI*GCKQYLQQRVQVLSEKK RRDYGIKQPLQHSDTMSAKLLLGSEHQPALQPP LQQPAARQNERREPGGGCATAAATAAAATAAAAA ATAAAAAGGGGGGCGGGGGCGGGSCSAPVAAAP RQPHHGGDHRRPPGRTRPHRQPQLPVLGAALAL ALQQDPARGLQGGSPRRGTRWDCGYCHGG*R*KL FC*APECLCCYEKPSSKVQRSEICGPEWTRQEF HLDHNRLHKSSPSSYLSQSN*SYSRWTSGTQKA QTEA**L*T*FVL*PPQ*FRAHSSSQYESRCPAS EPTALPELCTKSF*STRTESDYRPQAGTVFPAV VL*PVLPLLPEPDDVPVHPLYHPAVFHTGHWAS CHHRCA*AHFR**HCHL*LLPLAFHSQ*EEPGRC FRTGPFFRPQAVPKHFIPH*EPLLQPTNALSSH LYLHPASHLRHVPRYVRHHSLPHLPATTLPRLF PKPEWTLPDQQHSISLLWHFVRILSVSHGAGGRP VSFQNASAMHHHLEWQHAIKSKFA*PE*WC*R* WKPQQFPNCFEF*WQNG*ICLATILKFLSSGPV VSGGHIPHVSIYTYIERVHIYVYRLAIYKVPIF* KIFHSLTQS*SCSHKRVDIEKQKAQERQLQSSF RLFTI*DVLLQRNKEGKRYFCFCCLVCYHQ*PV HMPIQRGGLQVQEGEEQSRFLSVL*NFTPSRWQL CMDQCPPQTAHKTS*GALKGHEVEWMLPSQYHH SE*LFQFLLSDMLQVLI*TVGFVFFFFFPAPRK *LQK*LIRIDYFILLFNTPSPLFPLNQKEIPSLK PAFSFYAKLKMAIHYYSHNGIDSDCVWLCVVFF FFKL*ICVKSEVTC*REWSYNFKDIFIII**HL DP*NIS*CIDMLTSVSKSALY*ITNFFSGLEPYL KYC*AISVHPGTM*F*KILPSPNILDIDCFCDV *RKCYV*FFQIFECL*HSFAF*SGN*GFKKQPL ALYQHEMLE*CGFLISLG*L*LLSY*KDKHK*II ELQKNVLWFHS*AKL*IARLHSKDIVS*KPHMW IEGSIMRHLVQESKIAPEILNQPALPNNTTGEP QSALPKAPLAAFHRICI*RSRMTSLSFGSRSSEK AAGSSR*LS*EDIWPTGTYRQPLTFVFLTMEGQ GGQGAVEFGVY*CV*I*ARVLLWHAL*PLLAVT WQISKCKSLIQTRII**HHQANPCSPPRKVRVPS LESSTHPSRI*QRFAFKP*RPPCSLVLLKPTFV GHPALQDSVWGSWTCASCLGVSLGIHPDSF**K WMGKQNTLPSKGRIPSMLR*ISHFSIT*VRRK** LILFIVCVW*LEAHHCPFICKCKICVFVSAALK KASVWLHISILIN*HKNATASASCILFN*KGTI FVHYFLMLKGLF*VYSTHTE*L*GCFCVEMLL*W LQAATQKHFRSFFSLGKIQALLPSTLTPTTPMG VIHIS*NKFCPFLV*GLKFCFSFFLFFFFSLNP *FALGHVFDL*FQDQSKVLLLLWNHVLVPGN*NS VVLFVAQTLLEFAVFSMQPHFYPFQLSHKF*PI SEFQNRYHSFGFRLVE*HSAQN*ETQQINYRLL FRRSHCLSLARALSK*AEKSTPLGTICDKFRREE MCVQL*GFPQFRGKGLAQNPG*YMETRSISKSN NYTYGI*KNNNKRHFFQTLNLLFLENECI*KYF LCENFLDVCLLHVYK*LFAF*CSTLKYISQNHNL Q*FLRYSE*NSISFGYALPFLGFCGTKIFVAFC VNTSFHFYFFQLLLPKNCFPCTYCKNSALCHRS *LWMSLLLTSKGLFVLYVATVN*IIWHFSHDCNI NLKFEFNFQ*NGFFGFV