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Sequences

Sequence CBFA2T3.dAug05

CBFA2T3.F2 (forward primer)
CBFA2T3.R2 (reverse primer)
Single exon skipping

Frame 0

EWPEGRPRRAGPQMVPVREVAGAAAGLRPRHYH GDTAGCLTRRLQGDLPQPAAPGPPRVT*APGLPP PPQGRGHPVGGREGLGFPGTEAQLQDAPAGPAP PLSLKAAGGRERQSW*RPVLGSRLAPEGGPPHA GFKTEGQGSQFSLGIHLWLHVPDAPCAGERPPGI CRLLRTPGSQEGRPSPSGQES*GLSDAGLPSGG EDAAPVHTPQHAAPTACRIPGGHTPPLLHATHT DERQQPLTNSHQWCTVHTQRLQQWPGHL

Frame 1

NGPREGRAGQGPRWFLSGKWRAQLQASGPGIIT GTQLAASPAGCRETFPSLQPQARPASHEPQGSH PLPRAEDTQLVAGRASAFQGQRPNSRTPQLAQPL LFPSRLQEVGKGSPGRGLSWAPGWPLRVALLMP ASRLRDRAASSASGSTCGSMSQTHPVLESGLLA SAGCSAPRGPRKGGPAPVDRKAKASAMPDSPAEV KTQPRSTPPSMPPPPPAASQGATRPPSFTPHTL MNGSSHSPTAINGAPCTPNGFSNGPATS

Frame 2

MARGKAAQGRAPDGSCQGSGGRSCRPPAPALSR GHSWLPHPQAAGRPSPACSPRPAPRHMSPRAPT PSPGQRTPSWWPGGPRLSRDRGPTPGRPSWPSP SSFPQGCRRSGKAVLVEACPGLQVGP*GWPSSCR LQD*GTGQPVQPRDPPVAPCPRRTLCWRAASWH LPAAPHPGVPGRAAQPQWTGKLRPQRCRTPQRR *RRSPGPHPPACRPHRLPHPRGPHAPPPSRHTH* *TAAATHQQPSMVHRAHPTASAMARPPR

Sequence CBFA2T3.bAug05

GCGGCCGCCCCGCGCGCCCCAGCCCCGCCGCGCGCCTGAGCCCGGTGCGGCGCCAGAAGACAGCGCGCAGCCGCCCCTGAGTCGTGGAGGCGGGGACCAA GCTGGAAGGAGCAGCGACTCCCGGACCGAGTCGCGAGTGTGCGCCGTCCGCCGCCCGCCGGATCCCCCGGACCCCCCGACCCCCCAGTGGACAGGAAAGC TAAGGCCTCAGCGATGCCGGACTCCCCAGCGGAGGTGAAGACGCAGCCCCGGTCCACACCCCCCAGCATGCCGCCCCCACCGCCTGCCGCATCCCAGGGG GCCACACGCCCCCCCTCCTTCACGCCACACACACTGATGAACGGCAGCAGCCACTCACCAACAGCCATCAATGGTGCACCGTGCACACCCAACGGCTTCA GCAATGGCCCGGCCACCTCGTCCACAGCCTCCTTGTCCACACAGCACCTGCCCCCAGCCTGCGGGGCCCGGCAGCTCAGCAAGCTCAAGCGCTTCCTCAC CACACTGCAGCAGTTTGGCAGCGACATCTCCCCAGAGATTGGGGAGCGCGTGCGCACACTGGTGCTGGGCCTGGTGAACTCGACATTGACGATCGAGGAG TTTCATTCCAAGCTTCAGGAGGCCACCAACTTCCCTCTGCGGCCGTTTGTCATTCCCTTCCTGAAGGCAAACCTGCCCTTGCTGCAGCGGGAGCTCCTGC ACTGTGCACGCCTGGCCAAGCAGACGCCCGCCCAGTACTTGGCCCAGCATGAGCAGCTCCTGCTGGACGCCAGCGCCTCCTCCCCCATCGACTCCTCAGA GCTGCTACTGGAAGTCAACGAGAACGGCAAGAGGAGGACGCCCGACAGGACCAAAGAGAACGGGTCAGACCGCGACCCGCTGCACCCCGAGCACCTCAGC AAACGGCCATGCACCCTGAACCCTGCCCAGCGCTACAGCCCCAGCAACGGGCCACCGCAGCCCACACCGCCGCCGCACTACCGCCTGGAGGACATAGCCA TGGCCCACCACTTCCGAGATGCCTACCGCCACCCAGACCCCCGGGAGCTACGAGAGCGCCATCGGCCGCTTGTGGTGCCTGGGTCCCGGCAGGAAGAAGT GATCGACCACAAGCTCACAGAGCGTGAGTGGGCAGAAGAGTGGAAGCACCTCAACAACCTCCTGAACTGCATCATGGACATGGTGGAGAAGACGCGGCGC TCGCTCACGGTGCTGCGCAGGTGCCAGGAGGCCGACCGCGAGGAGCTCAACCACTGGGCGCGGCGCTACAGCGACGCCGAGGACACAAAGAAGGGCCCCG CTCCCGCCGCGGCCCGGCCCCGCAGCAGCTCCGCCGGTCCCGAAGGGCCTCAGCTAGACGTGCCTCGCGAGTTCCTGCCGAGGACCCTCACCGGCTACGT GCCTGAGGACATCTGGAGGAAGGCTGAAGAGGCCGTGAATGAGGTGAAGCGGCAGGCCATGTCGGAGCTGCAGAAAGCCGTGTCGGACGCGGAGCGCAAA GCGCACGAGCTCATCACCACGGAGCGTGCCAAGATGGAGCGGGCCCTGGCCGAGGCGAAGCGGCAGGCCTCCGAGGACGCCCTGACGGTCATCAACCAGC AGGAGGACTCCAGCGAGAGCTGCTGGAACTGCGGGCGGAAAGCCAGTGAGACGTGCAGCGGCTGCAACGCGGCACGCTACTGCGGGTCCTTCTGCCAGCA TCGGGACTGGGAGAAGCATCACCACGTGTGTGGCCAGAGCCTGCAGGGCCCCACAGCCGTGGTGGCCGACCCGGTGCCTGGACCGCCCGAAGCCGCCCAC AGCCTGGGCCCCTCCCTGCCTGTGGGTGCTGCCAGCCCCAGCGAAGCCGGCTCTGCGGGGCCTTCTCGCCCCGGCTCCCCCAGCCCACCTGGCCCACTGG ACACCGTGCCCCGCTGACCCCACTGGCCCCTGGCCTGCCGGACACAGCACCGTGCCAACCCCACCCAGCTCCAGGCCCACCGGATGCTGTGCCTGGCCTC CGATGCCTGGCCTGCCAGACACTGCGCCCCGCCTGACCTGGGGGAGCCGACCAATTAGTCACTGCTGCTACTGCCCCTCTCCGAAAGAAGACACAGAACC AACAAAACCGCATTCAGTGCACCTGCCTCAGCTACCTAATGATTCCGCGCGGAGACCTCCTGACAACGTCTCTTCAAGCATCCTCAGAAGCCTCGACTGA GCTTTAGACAGCAGAGCAGATGCCGCAGGCGCGGCGGCTCTGCCCACCTCTCTTTTCCTCTCTGTCTGTCTCTCCCCCTCTGTCTTCTCTATCCTCTCTC TCTCTATGACTATCACACACTTTCTCTTCAATGAAAAAATCGAATTGGTGGCTTATATTTTCAGCAAAGAATTTTGGGGGGTTTTGTGTGTTGGCAAAAG AGCTACTCAGAAATGGACAAAGAAAACGGGGGGGTTCTCCCCCTCCTGATTAAAAAGGGAGAAAGAAAACTGCGATTTTATAGCTGGAGATCTGAACCCA GCTGTGCCCCTCCCCCAGGGGCGTGAGGCTGATCAGCGAAGACGGGAGGAAAGATTTCGATTTCTGACTCAAGATGCATTTTTGGTTTCAGATTTTTTTT TCCTGTAATGTTAAACTCTTTGGCTTTAAGTAAAAATCCAAAAAGTTTTTTTAAAAAAGCAAAGGAAGCATACTTGTGAACTACCTTGCTAGCTAGCCAG CCAAGGATACCGGACACACCTCTGCTCCAAAGGAAATCCAAAAAAGCAAACACAAGAAATCAAAATCCAAAATTTGTTTGTCACTGCCAAAGTATTTTTT TCACTGTTTCACTTGCTCTTGGGTTTGTTTGGATGTGGGTCTTTTTCTCTTCTGTTCTGATTTTGTTTGTGGGTGTCGGGATATTTGGGTGCAGAGGGTT TGTGCCCAGTTAGAAGCGACTTTTGTTCTCTTCTGCGTAGGCGTTGGTGCGTCCGCCGCGTGTGCGTGGTCCGTGTGCCGTTGCTCCGGCCTGCGTCTCC ATATGTGTAGGAAAGGACACGCCGTCTGTCCTCACGCCCCCTGTGACTTTTCATATTTCCGTTTTCCACTTGTGGAAAAAAAGTGCTAAAGTTTTCTTCC CAGAGAGAGCATAATTCCGAAACAAAACTGTGACAATCTTTTGGGTTGATTCTCGACTGCTTTTCGAGCATGCGGAGCCAGCAGGCCTCCCTGAAACACT GCTTCTCGGCCAGCCCGTCCTCCTCTACCTCTCTCCTCTCCGCGCCCTCCGACCTCTCTCGGCCCCCTCACCCCAGCTCCGACCTCTCTCAGCCCCATCG CCCCAACTCCAACCTCTCGGCCCCATCGCCCCACCGCAGCTACTCCCCTTTCTTCCAAACTTTTGCAGAAAAAACAAAAAAACTACAAACAAAAGCAGCC CTCTGCCTCCTCCCCAGGGAAGACCCTGACCGTGTACATAGCCCTGGTGCTCCTGCCCAGCCACCCCTCAGATGCGTTCGCCTCTGGCCCTGGGGTGTGT CTCGGTGACGTTTTCTATCAGACGTGCTCCCTCCCATCCTCCAGCCCTGCCCACCCTCCCTCCACTCCTCTCAACTGCCTCAGCGATTTCAAGAAGGAAA TAAAGGGATAAAGAAATTCATGCTTGCACCGAGTACAAGGACAGACAGCAGGCACGGCCCGCAGCCTGGCATCTGTGCGTGTGGCGTGGCCCGTGGCTTG GCATCTGTGTGCGTGGTGTGGCCCGTGGCCTGGCATCTGTGTGCGTGGCGTGGCCCGTGGCCTGGCATCTGTGTGTGTGGCGTGGCCCGTGGCCTGGCAT CTGTGCGCGTGGCGTGGCCCGTGGCCTGGCATCTGTGTGCGTGGCTATCAGGAGTTCTAGGAACTCAGTGCAATACGGGAGTGACCCAGCTACTGAACCA GCCACGAACAGCCCGCCAGAGGCCTGAAGCTGAGCGTGTACGTTAATGTGAATGTATATAGTCTTTGCAGAGGTCCAAATGATATTCATGATGGTAATAA ACGAGATGTTTGCCAAATAAAAAACAGAAACCGCAGGA

CBFA2T3.F2 (forward primer)
CBFA2T3.R2 (reverse primer)
Single exon skipping

Frame 0

AAAPRAPAPPRA*ARCGARRQRAAAPESWRRGP SWKEQRLPDRVASVRRPPPAGSPGPPDPPVDRKA KASAMPDSPAEVKTQPRSTPPSMPPPPPAASQG ATRPPSFTPHTLMNGSSHSPTAINGAPCTPNGF SNGPATSSTASLSTQHLPPACGARQLSKLKRFLT TLQQFGSDISPEIGERVRTLVLGLVNSTLTIEE FHSKLQEATNFPLRPFVIPFLKANLPLLQRELL HCARLAKQTPAQYLAQHEQLLLDASASSPIDSSE LLLEVNENGKRRTPDRTKENGSDRDPLHPEHLS KRPCTLNPAQRYSPSNGPPQPTPPPHYRLEDIA MAHHFRDAYRHPDPRELRERHRPLVVPGSRQEEV IDHKLTEREWAEEWKHLNNLLNCIMDMVEKTRR SLTVLRRCQEADREELNHWARRYSDAEDTKKGP APAAARPRSSSAGPEGPQLDVPREFLPRTLTGYV PEDIWRKAEEAVNEVKRQAMSELQKAVSDAERK AHELITTERAKMERALAEAKRQASEDALTVINQ QEDSSESCWNCGRKASETCSGCNAARYCGSFCQH RDWEKHHHVCGQSLQGPTAVVADPVPGPPEAAH SLGPSLPVGAASPSEAGSAGPSRPGSPSPPGPL DTVPR*PHWPLACRTQHRANPTQLQAHRMLCLAS DAWPARHCAPPDLGEPTN*SLLLLPLSERRHRT NKTAFSAPASAT**FRAETS*QRLFKHPQKPRL SFRQQSRCRRRGGSAHLSFPLCLSLPLCLLYPLS LYDYHTLSLQ*KNRIGGLYFQQRILGGFVCWQK SYSEMDKENGGVLPLLIKKGERKLRFYSWRSEP SCAPPPGA*G*SAKTGGKISISDSRCIFGFRFFF PVMLNSLALSKNPKSFFKKAKEAYL*TTLLASQ PRIPDTPLLQRKSKKANTRNQNPKFVCHCQSIF FTVSLALGFVWMWVFFSSVLILFVGVGIFGCRGF VPS*KRLLFSSA*ALVRPPRVRGPCAVAPACVS ICVGKDTPSVLTPPVTFHISVFHLWKKSAKVFF PERA*FRNKTVTIFWVDSRLLFEHAEPAGLPETL LLGQPVLLYLSPLRALRPLSAPSPQLRPLSAPS PQLQPLGPIAPPQLLPFLPNFCRKNKKTTNKSS PLPPPQGRP*PCT*PWCSCPATPQMRSPLALGCV SVTFSIRRAPSHPPALPTLPPLLSTASAISRRK *RDKEIHACTEYKDRQQARPAAWHLCVWRGPWL GICVRGVARGLASVCVAWPVAWHLCVWRGPWPGI CARGVARGLASVCVAIRSSRNSVQYGSDPATEP ATNSPPEA*S*ACTLM*MYIVFAEVQMIFMMVI NEMFAK*KTETAG

Frame 1

RPPRAPQPRRAPEPGAAPEDSAQPPLSRGGGDQ AGRSSDSRTESRVCAVRRPPDPPDPPTPQWTGK LRPQRCRTPQRR*RRSPGPHPPACRPHRLPHPRG PHAPPPSRHTH**TAAATHQQPSMVHRAHPTAS AMARPPRPQPPCPHSTCPQPAGPGSSASSSASS PHCSSLAATSPQRLGSACAHWCWAW*TRH*RSRS FIPSFRRPPTSLCGRLSFPS*RQTCPCCSGSSC TVHAWPSRRPPSTWPSMSSSCWTPAPPPPSTPQ SCYWKSTRTARGGRPTGPKRTGQTATRCTPSTSA NGHAP*TLPSATAPATGHRSPHRRRTTAWRT*P WPTTSEMPTATQTPGSYESAIGRLWCLGPGRKK *STTSSQSVSGQKSGSTSTTS*TASWTWWRRRGA RSRCCAGARRPTARSSTTGRGATATPRTQRRAP LPPRPGPAAAPPVPKGLS*TCLASSCRGPSPAT CLRTSGGRLKRP*MR*SGRPCRSCRKPCRTRSAK RTSSSPRSVPRWSGPWPRRSGRPPRTP*RSSTS RRTPARAAGTAGGKPVRRAAAATRHATAGPSAS IGTGRSITTCVARACRAPQPWWPTRCLDRPKPPT AWAPPCLWVLPAPAKPALRGLLAPAPPAHLAHW TPCPADPTGPWPAGHSTVPTPPSSRPTGCCAWP PMPGLPDTAPRLTWGSRPISHCCYCPSPKEDTEP TKPHSVHLPQLPNDSARRPPDNVSSSILRSLD* ALDSRADAAGAAALPTSLFLSVCLSPSVFSILS LSMTITHFLFNEKIELVAYIFSKEFWGVLCVGKR ATQKWTKKTGGFSPS*LKREKENCDFIAGDLNP AVPLPQGREADQRRREERFRFLTQDAFLVSDFF FL*C*TLWL*VKIQKVFLKKQRKHTCELPC*LAS QGYRTHLCSKGNPKKQTQEIKIQNLFVTAKVFF SLFHLLLGLFGCGSFSLLF*FCLWVSGYLGAEG LCPVRSDFCSLLRRRWCVRRVCVVRVPLLRPASP YV*ERTRRLSSRPL*LFIFPFSTCGKKVLKFSS QREHNSETKL*QSFGLILDCFSSMRSQQASLKH CFSASPSSSTSLLSAPSDLSRPPHPSSDLSQPHR PNSNLSAPSPHRSYSPFFQTFAEKTKKLQTKAA LCLLPREDPDRVHSPGAPAQPPLRCVRLWPWGV SR*RFLSDVLPPILQPCPPSLHSSQLPQRFQEGN KGIKKFMLAPSTRTDSRHGPQPGICACGVARGL ASVCVVWPVAWHLCAWRGPWPGICVCGVARGLA SVRVAWPVAWHLCAWLSGVLGTQCNTGVTQLLNQ PRTARQRPEAERVR*CECI*SLQRSK*YS*W** TRCLPNKKQKPQ

Frame 2

GRPARPSPAARLSPVRRQKTARSRP*VVEAGTK LEGAATPGPSRECAPSAARRIPRTPRPPSGQES *GLSDAGLPSGGEDAAPVHTPQHAAPTACRIPG GHTPPLLHATHTDERQQPLTNSHQWCTVHTQRLQ QWPGHLVHSLLVHTAPAPSLRGPAAQQAQALPH HTAAVWQRHLPRDWGARAHTGAGPGELDIDDRG VSFQASGGHQLPSAAVCHSLPEGKPALAAAGAPA LCTPGQADARPVLGPA*AAPAGRQRLLPHRLLR AATGSQRERQEEDARQDQRERVRPRPAAPRAPQ QTAMHPEPCPALQPQQRATAAHTAAALPPGGHSH GPPLPRCLPPPRPPGATRAPSAACGAWVPAGRS DRPQAHRA*VGRRVEAPQQPPELHHGHGGEDAA LAHGAAQVPGGRPRGAQPLGAALQRRRGHKEGPR SRRGPAPQQLRRSRRASARRASRVPAEDPHRLR A*GHLEEG*RGRE*GEAAGHVGAAESRVGRGAQ SARAHHHGACQDGAGPGRGEAAGLRGRPDGHQPA GGLQRELLELRAESQ*DVQRLQRGTLLRVLLPA SGLGEASPRVWPEPAGPHSRGGRPGAWTARSRP QPGPLPACGCCQPQRSRLCGAFSPRLPQPTWPTG HRAPLTPLAPGLPDTAPCQPHPAPGPPDAVPGL RCLACQTLRPA*PGGADQLVTAATAPLRKKTQN QQNRIQCTCLSYLMIPRGDLLTTSLQASSEASTE L*TAEQMPQARRLCPPLFSSLSVSPPLSSLSSL SL*LSHTFSSMKKSNWWLIFSAKNFGGFCVLAK ELLRNGQRKRGGSPPPD*KGRKKTAIL*LEI*TQ LCPSPRGVRLISEDGRKDFDF*LKMHFWFQIFF SCNVKLFGFK*KSKKFF*KSKGSILVNYLAS*P AKDTGHTSAPKEIQKSKHKKSKSKICLSLPKYFF HCFTCSWVCLDVGLFLFCSDFVCGCRDIWVQRV CAQLEATFVLFCVGVGASAACAWSVCRCSGLRL HMCRKGHAVCPHAPCDFSYFRFPLVEKKC*SFLP RESIIPKQNCDNLLG*FSTAFRACGASRPP*NT ASRPARPPLPLSSPRPPTSLGPLTPAPTSLSPI APTPTSRPHRPTAATPLSSKLLQKKQKNYKQKQP SASSPGKTLTVYIALVLLPSHPSDAFASGPGVC LGDVFYQTCSLPSSSPAHPPSTPLNCLSDFKKE IKG*RNSCLHRVQGQTAGTARSLASVRVAWPVAW HLCAWCGPWPGICVRGVARGLASVCVAWPVAWH LCAWRGPWPGICVRGYQEF*ELSAIRE*PSY*T SHEQPARGLKLSVYVNVNVYSLCRGPNDIHDGNK RDVCQIKNRNRR

Sequence CBFA2T3.aAug05

GAATGGCCCGAGGGAAGGCCGCGCAGGGCAGGGCCCCAGATGGTTCCTGTCAGGGAAGTGGCGGGCGCAGCTGCAGGCCTCCGGCCCCGGCATTATCACG GGGACACAGCTGGCTGCCTCACCCGCAGGCTGCAGGGAGACCTTCCCCAGCCTGCAGCCCCAGGCCCGCCCCGCGTCACATGAGCCCCAGGGCTCCCACC CCCTCCCCAGGGCAGAGGACACCCAGTTGGTGGCCGGGAGGGCCTCGGCTTTCCAGGGACAGAGGCCCAACTCCAGGACGCCCCAGCTGGCCCAGCCCCT CCTCTTTCCCTCAAGGCTGCAGGAGGTCGGGAAAGGCAGTCCTGGTAGAGGCCTGTCCTGGGCTCCAGGTTGGCCCCTGAGGGTGGCCCTCCTCATGCCG GCTTCAAGACTGAGGGACAGGGCAGCCAGTTCAGCCTCGGGATCCACCTGTGGCTCCATGTCCCAGACGCACCCTGTGCTGGAGAGCGGCCTCCTGGCAT CTGCCGGCTGCTCCGCACCCCGGGGTCCCAGGAAGGGCGGCCCAGCCCCAGTGGACAGGAAAGCTAAGGCCTCAGCGATGCCGGACTCCCCAGCGGAGGT GAAGACGCAGCCCCGGTCCACACCCCCCAGCATGCCGCCCCCACCGCCTGCCGCATCCCAGGGGGCCACACGCCCCCCCTCCTTCACGCCACACACACAT CGAGAGGACGGGCCTGCGACGCTGCCCCACGGCCGTTTTCATGGCTGCTTAAAATGGTCTATGGTCTGTCTCTTGATGAACGGCAGCAGCCACTCACCAA CAGCCATCAATGGTGCACCGTGCACACCCAACGGCTTCAGCAATGGCCCGGCCACCTCGTCCACAGCCTCCTTGTCCACACAGCACCTGCCCCCAGCCTG CGGGGCCCGGCAGCTCAGCAAGCTCAAGCGCTTCCTCACCACACTGCAGCAGTTTGGCAGCGACATCTCCCCAGAGATTGGGGAGCGCGTGCGCACACTG GTGCTGGGCCTGGTGAACTCGACATTGACGATCGAGGAGTTTCATTCCAAGCTTCAGGAGGCCACCAACTTCCCTCTGCGGCCGTTTGTCATTCCCTTCC TGAAGGCAAACCTGCCCTTGCTGCAGCGGGAGCTCCTGCACTGTGCACGCCTGGCCAAGCAGACGCCCGCCCAGTACTTGGCCCAGCATGAGCAGCTCCT GCTGGACGCCAGCGCCTCCTCCCCCATCGACTCCTCAGAGCTGCTACTGGAAGTCAACGAGAACGGCAAGAGGAGGACGCCCGACAGGACCAAAGAGAAC GGGTCAGACCGCGACCCGCTGCACCCCGAGCACCTCAGCAAACGGCCATGCACCCTGAACCCTGCCCAGCGCTACAGCCCCAGCAACGGGCCACCGCAGC CCACACCGCCGCCGCACTACCGCCTGGAGGACATAGCCATGGCCCACCACTTCCGAGATGCCTACCGCCACCCAGACCCCCGGGAGCTACGAGAGCGCCA TCGGCCGCTTGTGGTGCCTGGGTCCCGGCAGGAAGAAGTGATCGACCACAAGCTCACAGAGCGTGAGTGGGCAGAAGAGTGGAAGCACCTCAACAACCTC CTGAACTGCATCATGGACATGGTGGAGAAGACGCGGCGCTCGCTCACGGTGCTGCGCAGGTGCCAGGAGGCCGACCGCGAGGAGCTCAACCACTGGGCGC GGCGCTACAGCGACGCCGAGGACACAAAGAAGGGCCCCGCTCCCGCCGCGGCCCGGCCCCGCAGCAGCTCCGCCGGTCCCGAAGGGCCTCAGCTAGACGT GCCTCGCGAGTTCCTGCCGAGGACCCTCACCGGCTACGTGCCTGAGGACATCTGGAGGAAGGCTGAAGAGGCCGTGAATGAGGTGAAGCGGCAGGCCATG TCGGAGCTGCAGAAAGCCGTGTCGGACGCGGAGCGCAAAGCGCACGAGCTCATCACCACGGAGCGTGCCAAGATGGAGCGGGCCCTGGCCGAGGCGAAGC GGCAGGCCTCCGAGGACGCCCTGACGGTCATCAACCAGCAGGAGGACTCCAGCGAGAGCTGCTGGAACTGCGGGCGGAAAGCCAGTGAGACGTGCAGCGG CTGCAACGCGGCACGCTACTGCGGGTCCTTCTGCCAGCATCGGGACTGGGAGAAGCATCACCACGTGTGTGGCCAGAGCCTGCAGGGCCCCACAGCCGTG GTGGCCGACCCGGTGCCTGGACCGCCCGAAGCCGCCCACAGCCTGGGCCCCTCCCTGCCTGTGGGTGCTGCCAGCCCCAGCGAAGCCGGCTCTGCGGGGC CTTCTCGCCCCGGCTCCCCCAGCCCACCTGGCCCACTGGACACCGTGCCCCGCTGACCCCACTGGCCCCTGGCCTGCCGGACACAGCACCGTGCCAACCC CACCCAGCTCCAGGCCCACCGGATGCTGTGCCTGGCCTCCGATGCCTGGCCTGCCAGACACTGCGCCCCGCCTGACCTGGGGGAGCCGACCAATTAGTCA CTGCTGCTACTGCCCCTCTCCGAAAGAAGACACAGAACCAACAAAACCGCATTCAGTGCACCTGCCTCAGCTACCTAATGATTCCGCGCGGAGACCTCCT GACAACGTCTCTTCAAGCATCCTCAGAAGCCTCGACTGAGCTTTAGACAGCAGAGCAGATGCCGCAGGCGCGGCGGCTCTGCCCACCTCTCTTTTCCTCT CTGTCTGTCTCTCCCCCTCTGTCTTCTCTATCCTCTCTCTCTCTATGACTATCACACACTTTCTCTTCAATGAAAAAATCGAATTGGTGGCTTATATTTT CAGCAAAGAATTTTGGGGGGTTTTGTGTGTTGGCAAAAGAGCTACTCAGAAATGGACAAAGAAAACGGGGGGGTTCTCCCCCTCCTGATTAAAAAGGGAG AAAGAAAACTGCGATTTTATAGCTGGAGATCTGAACCCAGCTGTGCCCCTCCCCCAGGGGCGTGAGGCTGATCAGCGAAGACGGGAGGAAAGATTTCGAT TTCTGACTCAAGATGCATTTTTGGTTTCAGATTTTTTTTTCCTGTAATGTTAAACTCTTTGGCTTTAAGTAAAAATCCAAAAAGTTTTTTTAAAAAAGCA AAGGAAGCATACTTGTGAACTACCTTGCTAGCTAGCCAGCCAAGGATACCGGACACACCTCTGCTCCAAAGGAAATCCAAAAAAGCAAACACAAGAAATC AAAATCCAAAATTTGTTTGTCACTGCCAAAGTATTTTTTTCACTGTTTCACTTGCTCTTGGGTTTGTTTGGATGTGGGTCTTTTTCTCTTCTGTTCTGAT TTTGTTTGTGGGTGTCGGGATATTTGGGTGCAGAGGGTTTGTGCCCAGTTAGAAGCGACTTTTGTTCTCTTCTGCGTAGGCGTTGGTGCGTCCGCCGCGT GTGCGTGGTCCGTGTGCCGTTGCTCCGGCCTGCGTCTCCATATGTGTAGGAAAGGACACGCCGTCTGTCCTCACGCCCCCTGTGACTTTTCATATTTCCG TTTTCCACTTGTGGAAAAAAAGTGCTAAAGTTTTCTTCCCAGAGAGAGCATAATTCCGAAACAAAACTGTGACAATCTTTTGGGTTGATTCTCGACTGCT TTTCGAGCATGCGGAGCCAGCAGGCCTCCCTGAAACACTGCTTCTCGGCCAGCCCGTCCTCCTCTACCTCTCTCCTCTCCGCGCCCTCCGACCTCTCTCG GCCCCCTCACCCCAGCTCCGACCTCTCTCAGCCCCATCGCCCCAACTCCAACCTCTCGGCCCCATCGCCCCACCGCAGCTACTCCCCTTTCTTCCAAACT TTTGCAGAAAAAACAAAAAAACTACAAACAAAAGCAGCCCTCTGCCTCCTCCCCAGGGAAGACCCTGACCGTGTACATAGCCCTGGTGCTCCTGCCCAGC CACCCCTCAGATGCGTTCGCCTCTGGCCCTGGGGTGTGTCTCGGTGACGTTTTCTATCAGACGTGCTCCCTCCCATCCTCCAGCCCTGCCCACCCTCCCT CCACTCCTCTCAACTGCCTCAGCGATTTCAAGAAGGAAATAAAGGGATAAAGAAATTCATGCTTGCACCGAGTACAAGGACAGACAGCAGGCACGGCCCG CAGCCTGGCATCTGTGCGTGTGGCGTGGCCCGTGGCTTGGCATCTGTGTGCGTGGTGTGGCCCGTGGCCTGGCATCTGTGTGCGTGGCGTGGCCCGTGGC CTGGCATCTGTGTGTGTGGCGTGGCCCGTGGCCTGGCATCTGTGCGCGTGGCGTGGCCCGTGGCCTGGCATCTGTGTGCGTGGCTATCAGGAGTTCTAGG AACTCAGTGCAATACGGGAGTGACCCAGCTACTGAACCAGCCACGAACAGCCCGCCAGAGGCCTGAAGCTGAGCGTGTACGTTAATGTGAATGTATATAG TCTTTGCAGAGGTCCAAATGATATTCATGATGGTAATAAACGAGATGTTTGCCAAATAAAAAACAGAAACCGCAGGA

CBFA2T3.F2 (forward primer)
CBFA2T3.R2 (reverse primer)
Single exon skipping

Frame 0

EWPEGRPRRAGPQMVPVREVAGAAAGLRPRHYH GDTAGCLTRRLQGDLPQPAAPGPPRVT*APGLPP PPQGRGHPVGGREGLGFPGTEAQLQDAPAGPAP PLSLKAAGGRERQSW*RPVLGSRLAPEGGPPHA GFKTEGQGSQFSLGIHLWLHVPDAPCAGERPPGI CRLLRTPGSQEGRPSPSGQES*GLSDAGLPSGG EDAAPVHTPQHAAPTACRIPGGHTPPLLHATHT SRGRACDAAPRPFSWLLKMVYGLSLDERQQPLTN SHQWCTVHTQRLQQWPGHLVHSLLVHTAPAPSL RGPAAQQAQALPHHTAAVWQRHLPRDWGARAHT GAGPGELDIDDRGVSFQASGGHQLPSAAVCHSLP EGKPALAAAGAPALCTPGQADARPVLGPA*AAP AGRQRLLPHRLLRAATGSQRERQEEDARQDQRE RVRPRPAAPRAPQQTAMHPEPCPALQPQQRATAA HTAAALPPGGHSHGPPLPRCLPPPRPPGATRAP SAACGAWVPAGRSDRPQAHRA*VGRRVEAPQQP PELHHGHGGEDAALAHGAAQVPGGRPRGAQPLGA ALQRRRGHKEGPRSRRGPAPQQLRRSRRASARR ASRVPAEDPHRLRA*GHLEEG*RGRE*GEAAGH VGAAESRVGRGAQSARAHHHGACQDGAGPGRGEA AGLRGRPDGHQPAGGLQRELLELRAESQ*DVQR LQRGTLLRVLLPASGLGEASPRVWPEPAGPHSR GGRPGAWTARSRPQPGPLPACGCCQPQRSRLCGA FSPRLPQPTWPTGHRAPLTPLAPGLPDTAPCQP HPAPGPPDAVPGLRCLACQTLRPA*PGGADQLV TAATAPLRKKTQNQQNRIQCTCLSYLMIPRGDLL TTSLQASSEASTEL*TAEQMPQARRLCPPLFSS LSVSPPLSSLSSLSL*LSHTFSSMKKSNWWLIF SAKNFGGFCVLAKELLRNGQRKRGGSPPPD*KGR KKTAIL*LEI*TQLCPSPRGVRLISEDGRKDFD F*LKMHFWFQIFFSCNVKLFGFK*KSKKFF*KS KGSILVNYLAS*PAKDTGHTSAPKEIQKSKHKKS KSKICLSLPKYFFHCFTCSWVCLDVGLFLFCSD FVCGCRDIWVQRVCAQLEATFVLFCVGVGASAA CAWSVCRCSGLRLHMCRKGHAVCPHAPCDFSYFR FPLVEKKC*SFLPRESIIPKQNCDNLLG*FSTA FRACGASRPP*NTASRPARPPLPLSSPRPPTSL GPLTPAPTSLSPIAPTPTSRPHRPTAATPLSSKL LQKKQKNYKQKQPSASSPGKTLTVYIALVLLPS HPSDAFASGPGVCLGDVFYQTCSLPSSSPAHPP STPLNCLSDFKKEIKG*RNSCLHRVQGQTAGTAR SLASVRVAWPVAWHLCAWCGPWPGICVRGVARG LASVCVAWPVAWHLCAWRGPWPGICVRGYQEF* ELSAIRE*PSY*TSHEQPARGLKLSVYVNVNVYS LCRGPNDIHDGNKRDVCQIKNRNRR

Frame 1

NGPREGRAGQGPRWFLSGKWRAQLQASGPGIIT GTQLAASPAGCRETFPSLQPQARPASHEPQGSH PLPRAEDTQLVAGRASAFQGQRPNSRTPQLAQPL LFPSRLQEVGKGSPGRGLSWAPGWPLRVALLMP ASRLRDRAASSASGSTCGSMSQTHPVLESGLLA SAGCSAPRGPRKGGPAPVDRKAKASAMPDSPAEV KTQPRSTPPSMPPPPPAASQGATRPPSFTPHTH REDGPATLPHGRFHGCLKWSMVCLLMNGSSHSP TAINGAPCTPNGFSNGPATSSTASLSTQHLPPAC GARQLSKLKRFLTTLQQFGSDISPEIGERVRTL VLGLVNSTLTIEEFHSKLQEATNFPLRPFVIPF LKANLPLLQRELLHCARLAKQTPAQYLAQHEQLL LDASASSPIDSSELLLEVNENGKRRTPDRTKEN GSDRDPLHPEHLSKRPCTLNPAQRYSPSNGPPQ PTPPPHYRLEDIAMAHHFRDAYRHPDPRELRERH RPLVVPGSRQEEVIDHKLTEREWAEEWKHLNNL LNCIMDMVEKTRRSLTVLRRCQEADREELNHWA RRYSDAEDTKKGPAPAAARPRSSSAGPEGPQLDV PREFLPRTLTGYVPEDIWRKAEEAVNEVKRQAM SELQKAVSDAERKAHELITTERAKMERALAEAK RQASEDALTVINQQEDSSESCWNCGRKASETCSG CNAARYCGSFCQHRDWEKHHHVCGQSLQGPTAV VADPVPGPPEAAHSLGPSLPVGAASPSEAGSAG PSRPGSPSPPGPLDTVPR*PHWPLACRTQHRANP TQLQAHRMLCLASDAWPARHCAPPDLGEPTN*S LLLLPLSERRHRTNKTAFSAPASAT**FRAETS *QRLFKHPQKPRLSFRQQSRCRRRGGSAHLSFPL CLSLPLCLLYPLSLYDYHTLSLQ*KNRIGGLYF QQRILGGFVCWQKSYSEMDKENGGVLPLLIKKG ERKLRFYSWRSEPSCAPPPGA*G*SAKTGGKISI SDSRCIFGFRFFFPVMLNSLALSKNPKSFFKKA KEAYL*TTLLASQPRIPDTPLLQRKSKKANTRN QNPKFVCHCQSIFFTVSLALGFVWMWVFFSSVLI LFVGVGIFGCRGFVPS*KRLLFSSA*ALVRPPR VRGPCAVAPACVSICVGKDTPSVLTPPVTFHIS VFHLWKKSAKVFFPERA*FRNKTVTIFWVDSRLL FEHAEPAGLPETLLLGQPVLLYLSPLRALRPLS APSPQLRPLSAPSPQLQPLGPIAPPQLLPFLPN FCRKNKKTTNKSSPLPPPQGRP*PCT*PWCSCPA TPQMRSPLALGCVSVTFSIRRAPSHPPALPTLP PLLSTASAISRRK*RDKEIHACTEYKDRQQARP AAWHLCVWRGPWLGICVRGVARGLASVCVAWPVA WHLCVWRGPWPGICARGVARGLASVCVAIRSSR NSVQYGSDPATEPATNSPPEA*S*ACTLM*MYI VFAEVQMIFMMVINEMFAK*KTETAG

Frame 2

MARGKAAQGRAPDGSCQGSGGRSCRPPAPALSR GHSWLPHPQAAGRPSPACSPRPAPRHMSPRAPT PSPGQRTPSWWPGGPRLSRDRGPTPGRPSWPSP SSFPQGCRRSGKAVLVEACPGLQVGP*GWPSSCR LQD*GTGQPVQPRDPPVAPCPRRTLCWRAASWH LPAAPHPGVPGRAAQPQWTGKLRPQRCRTPQRR *RRSPGPHPPACRPHRLPHPRGPHAPPPSRHTHI ERTGLRRCPTAVFMAA*NGLWSVS**TAAATHQ QPSMVHRAHPTASAMARPPRPQPPCPHSTCPQP AGPGSSASSSASSPHCSSLAATSPQRLGSACAHW CWAW*TRH*RSRSFIPSFRRPPTSLCGRLSFPS *RQTCPCCSGSSCTVHAWPSRRPPSTWPSMSSS CWTPAPPPPSTPQSCYWKSTRTARGGRPTGPKRT GQTATRCTPSTSANGHAP*TLPSATAPATGHRS PHRRRTTAWRT*PWPTTSEMPTATQTPGSYESA IGRLWCLGPGRKK*STTSSQSVSGQKSGSTSTTS *TASWTWWRRRGARSRCCAGARRPTARSSTTGR GATATPRTQRRAPLPPRPGPAAAPPVPKGLS*T CLASSCRGPSPATCLRTSGGRLKRP*MR*SGRPC RSCRKPCRTRSAKRTSSSPRSVPRWSGPWPRRS GRPPRTP*RSSTSRRTPARAAGTAGGKPVRRAA AATRHATAGPSASIGTGRSITTCVARACRAPQPW WPTRCLDRPKPPTAWAPPCLWVLPAPAKPALRG LLAPAPPAHLAHWTPCPADPTGPWPAGHSTVPT PPSSRPTGCCAWPPMPGLPDTAPRLTWGSRPISH CCYCPSPKEDTEPTKPHSVHLPQLPNDSARRPP DNVSSSILRSLD*ALDSRADAAGAAALPTSLFL SVCLSPSVFSILSLSMTITHFLFNEKIELVAYIF SKEFWGVLCVGKRATQKWTKKTGGFSPS*LKRE KENCDFIAGDLNPAVPLPQGREADQRRREERFR FLTQDAFLVSDFFFL*C*TLWL*VKIQKVFLKKQ RKHTCELPC*LASQGYRTHLCSKGNPKKQTQEI KIQNLFVTAKVFFSLFHLLLGLFGCGSFSLLF* FCLWVSGYLGAEGLCPVRSDFCSLLRRRWCVRRV CVVRVPLLRPASPYV*ERTRRLSSRPL*LFIFP FSTCGKKVLKFSSQREHNSETKL*QSFGLILDC FSSMRSQQASLKHCFSASPSSSTSLLSAPSDLSR PPHPSSDLSQPHRPNSNLSAPSPHRSYSPFFQT FAEKTKKLQTKAALCLLPREDPDRVHSPGAPAQ PPLRCVRLWPWGVSR*RFLSDVLPPILQPCPPSL HSSQLPQRFQEGNKGIKKFMLAPSTRTDSRHGP QPGICACGVARGLASVCVVWPVAWHLCAWRGPW PGICVCGVARGLASVRVAWPVAWHLCAWLSGVLG TQCNTGVTQLLNQPRTARQRPEAERVR*CECI* SLQRSK*YS*W**TRCLPNKKQKPQ