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Sequences

Sequence VDR.cAug05

CTGCTTGTCAAAAGGCGGCAGCGGAGCCGTGTGCGCCGGGAGCGCGGAACAGCTTGTCCACCCGCCGGCCGGACCAGAAGCCTTTGGGTCTGAAGTGTCT
GTGAGACCTCACAGAAGAGCACCCCTGGGCTCCACTTACCTGCCCCCTGCTCCTTCAGGGATGGAGGCAATGGCGGCCAGCACTTCCCTGCCTGACCCTG
GAGACTTTGACCGGAACGTGCCCCGGATCTGTGGGGTGTGTGGAGACCGAGCCACTGGCTTTCACTTCAATGCTATGACCTGTGAAGGCTGCAAAGGCTT
CTTCAGGCGAAGCATGAAGCGGAAGGCACTATTCACCTGCCCCTTCAACGGGGACTGCCGCATCACCAAGGACAACCGACGCCACTGCCAGGCCTGCCGG
CTCAAACGCTGTGTGGACATCGGCATGATGAAGGAGTTCATTCTGACAGATGAGGAAGTGCAGAGGAAGCGGGAGATGATCCTGAAGCGGAAGGAGGAGG
AGGCCTTGAAGGACAGTCTGCGGCCCAAGCTGTCTGAGGAGCAGCAGCGCATCATTGCCATACTGCTGGACGCCCACCATAAGACCTACGACCCCACCTA
CTCCGACTTCTGCCAGTTCCGGCCTCCAGTTCGTGTGAATGATGGTGGAGGGAGCCATCCTTCCAGGCCCAACTCCAGACACACTCCCAGCTTCTCTGGG
GACTCCTCCTCCTCCTGCTCAGATCACTGTATCACCTCTTCAGACATGATGGACTCGTCCAGCTTCTCCAATCTGGATCTGAGTGAAGAAGATTCAGATG
ACCCTTCTGTGACCCTAGAGCTGTCCCAGCTCTCCATGCTGCCCCACCTGGCTGACCTGGTCAGTTACAGCATCCAAAAGGTCATTGGCTTTGCTAAGAT
GATACCAGGATTCAGAGACCTCACCTCTGAGGACCAGATCGTACTGCTGAAGTCAAGTGCCATTGAGGTCATCATGTTGCGCTCCAATGAGTCCTTCACC
ATGGACGACATGTCCTGGACCTGTGGCAACCAAGACTACAAGTACCGCGTCAGTGACGTGACCAAAGCCGGACACAGCCTGGAGCTGATTGAGCCCCTCA
TCAAGTTCCAGGTGGGACTGAAGAAGCTGAACTTGCATGAGGAGGAGCATGTCCTGCTCATGGCCATCTGCATCGTCTCCCCAGATCGTCCTGGGGTGCA
GGACGCCGCGCTGATTGAGGCCATCCAGGACCGCCTGTCCAACACACTGCAGACGTACATCCGCTGCCGCCACCCGCCCCCGGGCAGCCACCTGCTCTAT
GCCAAGATGATCCAGAAGCTAGCCGACCTGCGCAGCCTCAATGAGGAGCACTCCAAGCAGTACCGCTGCCTCTCCTTCCAGCCTGAGTGCAGCATGAAGC
TAACGCCCCTTGTGCTCGAAGTGTTTGGCAATGAGATCTCCTGACTAGGACAGCCTGTGGCGGTGCCTGGGTGGGGCTGCTCCTCCAGGGCCACGTGCCA
GGCCCGGGGCTGGCGGCTACTCAGCAGCCCTCCTCACCCCGTCTGGGGTTCAGCCCCTCCTCTGCCACCTCCCCTATCCACCCAGCCCATTCTCTCTCCT
GTCCAACCTAACCCCTTTCCTGCGGGCTTTTCCCCGGTCCCTTGAGACCTCAGCCATGAGGAGTTGCTGTTTGTTTGACAAAGAAACCCAAGTGGGGGCA
GAGGGCAGAGGCTGGAGGCAGGGCCTTGCCCAGAGATGCCTCCACCGCTGCCTAAGTGGCTGCTGACTGATGTTGAGGGAACAGACAGGAGAAATGCATC
CATTCCTCAGGGACAGAGACACCTGCACCTCCCCCCACTGCAGGCCCCGCTTGTCCAGCGCCTAGTGGGGTCTCCCTCTCCTGCCTACTCACGATAAATA
ATCGGCCCACAGCTCCCACCCCACCCCCTTCAGTGCCCACCAACATCCCATTGCCCTGGTTATATTCTCACGGGCAGTAGCTGTGGTGAGGTGGGTTTTC
TTCCCATCACTGGAGCACCAGGCACGAACCCACCTGCTGAGAGACCCAAGGAGGAAAAACAGACAAAAACAGCCTCACAGAAGAATATGACAGCTGTCCC
TGTCACCAAGCTCACAGTTCCTCGCCCTGGGTCTAAGGGGTTGGTTGAGGTGGAAGCCCTCCTTCCACGGATCCATGTAGCAGGACTGAATTGTCCCCAG
TTTGCAGAAAAGCACCTGCCGACCTCGTCCTCCCCCTGCCAGTGCCTTACCTCCTGCCCAGGAGAGCCAGCCCTCCCTGTCCTCCTCGGATCACCGAGAG
TAGCCGAGAGCCTGCTCCCCCACCCCCTCCCCAGGGGAGAGGGTCTGGAGAAGCAGTGAGCCGCATCTTCTCCATCTGGCAGGGTGGGATGGAGGAGAAG
AATTTTCAGACCCCAGCGGCTGAGTCATGATCTCCCTGCCGCCTCAATGTGGTTGCAAGGCCGCTGTTCACCCACAGGGCTAAGAGCTAGCGCTGCCGCA
CCCCAGAGTGTGGGAAGGGAGAGCGGGGCAGTCTCGGGTGGCTAGTCAGAGAGAGTGTTTGGGGGTTCCGTGATGTAGGGTAAGGTGCCTTCTTATTCTC
ACTCCACCACCCAAAAGTCAAAAGGTGCCTGTGAGGCAGGGGCGGAGTGATACAACTTCAAGTGCATGCTCTCTGCAGCCAGCCCAGCCCAGCTGGTGGG
AAGCGTCTGTCCGTTTACTCCAAGGTGGGGTCTTTGTGAGAGTGAGCTGTAGGTGTGCGGGACCGGTACAGAAAGGCGTTCTTCGAGGTGGATCACAGAG
GCTTCTTCAGATCAGTGCTTGAGTTTGGGGAATGCGGCCGCATTCCCTGAGTCACCAGGAATGTTAAAGTCAGTGGGAACGTGACTGCCCCAACTCCTGG
AAGCTGTGTCCTTGCACCTGCATCCGTAGTTCCCTGAAAACCCAGAGAGGAATCAGACTTCACACTGCAAGAGCCTTGGTGTCCACCTGGCCCCATGTCT
CTCAGAATTCTTCAGGTGGAAAAACATCTGAAAGCCACGTTCCTTACTGCAGAATAGCATATATATCGCTTAATCTTAAATTTATTAGATATGAGTTGTT
TTCAGACTCAGACTCCATTTGTATTATAGTCTAATATACAGGGTAGCAGGTACCACTGATTTGGAGATATTTATGGGGGGAGAACTTACATTGTGAAACT
TCTGTACATTAATTATTATTGCTGTTGTTATTTTACAAGGGTCTAGGGAGAGACCCTTGTTTGATTTTAGCTGCAGAACGTATTGGTCCAGCTTGCTCTT
CAGTGGGAGAAAACACTTGTAAGTTGCTAAACGAGTCAATCCCCTCATTCAGGAAAACTGACAGAGGAGGGCGTGACTCACCCAAGCATATATAACTAGC
TAGAAGTGGGCCAGGACAGGCCCGGCGCGGTGGCTCACGCCTGTAATCCCAGCAGTTTGGGAGGTCGAGGTAGGTGGATCACCTGAGGTCGGGAGTTCGA
GACCAACCTGACCAACATGGAGAAACCCTGTCTCTATTAAAAATACAAAAAAAAAAAAAAAAAAAATAGCCGGGCATGGTGGCGCAAGCCTGTAATCCCA
GCTACTCAGGAGGCTGAGGCAGAAGAATTGAACCCAGGAGGTGGAGGTTGCAGTGAGCTGAGATCGTGCCGTTACTCTCCAACCTGGACAACAAGAGCGA
AACTCCGTCTTAGAAGTGGACCAGGACAGGACCAGATTTTGGAGTCATGGTCCGGTGTCCTTTTCACTACACCATGTTTGAGCTCAGACCCCCACTCTCA
TTCCCCAGGTGGCTGACCCAGTCCCTGGGGGAAGCCCTGGATTTCAGAAAGAGCAAGTCTGGATCTGGGACCCTTTCCTTCCTTCCCTGGCTTGTAACTC
CACCAACCCATCAGAAGGAGAAGGAAGGAGACTCACCTCTGCCTCAATGTGAATCAGACCCTACCCCACCACGATGTGGCCCTGGCCTGCTGGGCTCTCC
ACCTCAGCCTTGGATAATGCTGTTGCCTCATCTATAACATGCATTTGTCTTTGTAATGTCACCACCTTCCCAGCTCTCCCTCTGGCCCTGCCTTCTTCGG
GGAACTCCTGGAAATATCAGTTACTCAGCCCTGGGCCCCACCACCTAGGCCACTCCTCCAAAGGAAGTCTAGGAGCTGGGAGGAAAAGAAAAGAGGGGAA
AATGAGTTTTTATGGGGCTGAACGGGGAGAAAAGGTCATCATCGATTCTACTTTAGAATGAGAGTGTGAAATAGACATTTGTAAATGTAAAACTTTTAAG
GTATATCATTATAACTGAAGGAGAAGGTGCCCCAAAATGCAAGATTTTCCACAAGATTCCCAGAGACAGGAAAATCCTCTGGCTGGCTAACTGGAAGCAT
GTAGGAGAATCCAAGCGAGGTCAACAGAGAAGGCAGGAATGTGTGGCAGATTTAGTGAAAGCTAGAGATATGGCAGCGAAAGGATGTAAACAGTGCCTGC
TGAATGATTTCCAAAGAGAAAAAAAGTTTGCCAGAAGTTTGTCAAGTCAACCAATGTAGAAAGCTTTGCTTATGGTAATAAAAATGGCTCATACTTATAT
AGCACTTACTTTGTTGCAAGTACTGCTGTAAATAAATGCTTTATGCAAACCAA
  • VDR.F2 (forward primer)
  • VDR.R2 (reverse primer)
  • Alternative donor

Frame 0

LLVKRRQRSRVRRERGTACPPAGRTRSLWV*SV
CETSQKSTPGLHLPAPCSFRDGGNGGQHFPA*PW
RL*PERAPDLWGVWRPSHWLSLQCYDL*RLQRL
LQAKHEAEGTIHLPLQRGLPHHQGQPTPLPGLP
AQTLCGHRHDEGVHSDR*GSAEEAGDDPEAEGGG
GLEGQSAAQAV*GAAAHHCHTAGRPP*DLRPHL
LRLLPVPASSSCE*WWREPSFQAQLQTHSQLLW
GLLLLLLRSLYHLFRHDGLVQLLQSGSE*RRFR*
PFCDPRAVPALHAAPPG*PGQLQHPKGHWLC*D
DTRIQRPHL*GPDRTAEVKCH*GHHVALQ*VLH
HGRHVLDLWQPRLQVPRQ*RDQSRTQPGAD*APH
QVPGGTEEAELA*GGACPAHGHLHRLPRSSWGA
GRRAD*GHPGPPVQHTADVHPLPPPAPGQPPAL
CQDDPEASRPAQPQ*GALQAVPLPLLPA*VQHEA
NAPCARSVWQ*DLLTRTACGGAWVGLLLQGHVP
GPGLAATQQPSSPRLGFSPSSATSPIHPAHSLS
CPT*PLSCGLFPGPLRPQP*GVAVCLTKKPKWGQ
RAEAGGRALPRDASTAA*VAAD*C*GNRQEKCI
HSSGTETPAPPPTAGPACPAPSGVSLSCLLTIN
NRPTAPTPPPSVPTNIPLPWLYSHGQ*LW*GGFS
SHHWSTRHEPTC*ETQGGKTDKNSLTEEYDSCP
CHQAHSSSPWV*GVG*GGSPPSTDPCSRTELSP
VCRKAPADLVLPLPVPYLLPRRASPPCPPRITES
SREPAPPPPPQGRGSGEAVSRIFSIWQGGMEEK
NFQTPAAES*SPCRLNVVARPLFTHRAKS*RCR
TPECGKGERGSLGWLVRESVWGFRDVG*GAFLFS
LHHPKVKRCL*GRGGVIQLQVHALCSQPSPAGG
KRLSVYSKVGSL*E*AVGVRDRYRKAFFEVDHR
GFFRSVLEFGECGRIP*VTRNVKVSGNVTAPTPG
SCVLAPASVVP*KPREESDFTLQEPWCPPGPMS
LRILQVEKHLKATFLTAE*HIYRLILNLLDMSC
FQTQTPFVL*SNIQGSRYH*FGDIYGGRTYIVKL
LYINYYCCCYFTRV*GETLV*F*LQNVLVQLAL
QWEKTLVSC*TSQSPHSGKLTEEGVTHPSIYN*
LEVGQDRPGAVAHACNPSSLGGRGRWIT*GREFE
TNLTNMEKPCLY*KYKKKKKKNSRAWWRKPVIP
ATQEAEAEELNPGGGGCSELRSCRYSPTWTTRA
KLRLRSGPGQDQILESWSGVLFTTPCLSSDPHSH
SPGG*PSPWGKPWISERASLDLGPFPSFPGL*L
HQPIRRRRKETHLCLNVNQTLPHHDVALACWAL
HLSLG*CCCLIYNMHLSL*CHHLPSSPSGPAFFG
ELLEISVTQPWAPPPRPLLQRKSRSWEEKKRGE
NEFLWG*TGRKGHHRFYFRMRV*NRHL*M*NF*
GISL*LKEKVPQNARFSTRFPETGKSSGWLTGSM
*ENPSEVNREGRNVWQI**KLEIWQRKDVNSAC
*MISKEKKSLPEVCQVNQCRKLCLW**KWLILI
*HLLCCKYCCK*MLYANQ

Frame 1

CLSKGGSGAVCAGSAEQLVHPPAGPEAFGSEVS
VRPHRRAPLGSTYLPPAPSGMEAMAASTSLPDP
GDFDRNVPRICGVCGDRATGFHFNAMTCEGCKGF
FRRSMKRKALFTCPFNGDCRITKDNRRHCQACR
LKRCVDIGMMKEFILTDEEVQRKREMILKRKEE
EALKDSLRPKLSEEQQRIIAILLDAHHKTYDPTY
SDFCQFRPPVRVNDGGGSHPSRPNSRHTPSFSG
DSSSSCSDHCITSSDMMDSSSFSNLDLSEEDSD
DPSVTLELSQLSMLPHLADLVSYSIQKVIGFAKM
IPGFRDLTSEDQIVLLKSSAIEVIMLRSNESFT
MDDMSWTCGNQDYKYRVSDVTKAGHSLELIEPL
IKFQVGLKKLNLHEEEHVLLMAICIVSPDRPGVQ
DAALIEAIQDRLSNTLQTYIRCRHPPPGSHLLY
AKMIQKLADLRSLNEEHSKQYRCLSFQPECSMK
LTPLVLEVFGNEIS*LGQPVAVPGWGCSSRATCQ
ARGWRLLSSPPHPVWGSAPPLPPPLSTQPILSP
VQPNPFPAGFSPVP*DLSHEELLFV*QRNPSGG
RGQRLEAGPCPEMPPPLPKWLLTDVEGTDRRNAS
IPQGQRHLHLPPLQAPLVQRLVGSPSPAYSR*I
IGPQLPPHPLQCPPTSHCPGYILTGSSCGEVGF
LPITGAPGTNPPAERPKEEKQTKTASQKNMTAVP
VTKLTVPRPGSKGLVEVEALLPRIHVAGLNCPQ
FAEKHLPTSSSPCQCLTSCPGEPALPVLLGSPR
VAESLLPHPLPRGEGLEKQ*AASSPSGRVGWRRR
IFRPQRLSHDLPAASMWLQGRCSPTGLRASAAA
PQSVGRESGAVSGG*SERVFGGSVM*GKVPSYS
HSTTQKSKGACEAGAE*YNFKCMLSAASPAQLVG
SVCPFTPRWGLCESEL*VCGTGTERRSSRWITE
ASSDQCLSLGNAAAFPESPGMLKSVGT*LPQLL
EAVSLHLHP*FPENPERNQTSHCKSLGVHLAPCL
SEFFRWKNI*KPRSLLQNSIYIA*S*IY*I*VV
FRLRLHLYYSLIYRVAGTTDLEIFMGGELTL*N
FCTLIIIAVVILQGSRERPLFDFSCRTYWSSLLF
SGRKHL*VAKRVNPLIQEN*QRRA*LTQAYITS
*KWARTGPARWLTPVIPAVWEVEVGGSPEVGSS
RPT*PTWRNPVSIKNTKKKKKKIAGHGGASL*SQ
LLRRLRQKN*TQEVEVAVS*DRAVTLQPGQQER
NSVLEVDQDRTRFWSHGPVSFSLHHV*AQTPTL
IPQVADPVPGGSPGFQKEQVWIWDPFLPSLACNS
TNPSEGEGRRLTSASM*IRPYPTTMWPWPAGLS
TSALDNAVASSITCICLCNVTTFPALPLALPSS
GNSWKYQLLSPGPHHLGHSSKGSLGAGRKRKEGK
MSFYGAERGEKVIIDSTLE*ECEIDICKCKTFK
VYHYN*RRRCPKMQDFPQDSQRQENPLAG*LEA
CRRIQARSTEKAGMCGRFSES*RYGSERM*TVPA
E*FPKRKKVCQKFVKSTNVESFAYGNKNGSYLY
STYFVASTAVNKCFMQT

Frame 2

ACQKAAAEPCAPGARNSLSTRRPDQKPLGLKCL
*DLTEEHPWAPLTCPLLLQGWRQWRPALPCLTL
ETLTGTCPGSVGCVETEPLAFTSML*PVKAAKA
SSGEA*SGRHYSPAPSTGTAASPRTTDATARPAG
SNAVWTSA**RSSF*QMRKCRGSGR*S*SGRRR
RP*RTVCGPSCLRSSSASLPYCWTPTIRPTTPP
TPTSASSGLQFV*MMVEGAILPGPTPDTLPASLG
TPPPPAQITVSPLQT*WTRPASPIWI*VKKIQM
TLL*P*SCPSSPCCPTWLTWSVTASKRSLALLR
*YQDSETSPLRTRSYC*SQVPLRSSCCAPMSPSP
WTTCPGPVATKTTSTASVT*PKPDTAWS*LSPS
SSSRWD*RS*TCMRRSMSCSWPSASSPQIVLGC
RTPR*LRPSRTACPTHCRRTSAAATRPRAATCSM
PR*SRS*PTCAASMRSTPSSTAASPSSLSAA*S
*RPLCSKCLAMRSPD*DSLWRCLGGAAPPGPRA
RPGAGGYSAALLTPSGVQPLLCHLPYPPSPFSLL
SNLTPFLRAFPRSLETSAMRSCCLFDKETQVGA
EGRGWRQGLAQRCLHRCLSGC*LMLREQTGEMH
PFLRDRDTCTSPHCRPRLSSA*WGLPLLPTHDK*
SAHSSHPTPFSAHQHPIALVIFSRAVAVVRWVF
FPSLEHQARTHLLRDPRRKNRQKQPHRRI*QLS
LSPSSQFLALGLRGWLRWKPSFHGSM*QD*IVPS
LQKSTCRPRPPPASALPPAQESQPSLSSSDHRE
*PRACSPTPSPGERVWRSSEPHLLHLAGWDGGE
EFSDPSG*VMISLPPQCGCKAAVHPQG*ELALPH
PRVWEGRAGQSRVASQRECLGVP*CRVRCLLIL
TPPPKSQKVPVRQGRSDTTSSACSLQPAQPSWW
EASVRLLQGGVFVRVSCRCAGPVQKGVLRGGSQR
LLQISA*VWGMRPHSLSHQEC*SQWERDCPNSW
KLCPCTCIRSSLKTQRGIRLHTARALVSTWPHV
SQNSSGGKTSESHVPYCRIAYISLNLKFIRYELF
SDSDSICIIV*YTG*QVPLIWRYLWGENLHCET
SVH*LLLLLLFYKGLGRDPCLILAAERIGPACS
SVGENTCKLLNESIPSFRKTDRGGRDSPKHI*LA
RSGPGQARRGGSRL*SQQFGRSR*VDHLRSGVR
DQPDQHGETLSLLKIQKKKKKK*PGMVAQACNP
SYSGG*GRRIEPRRWRLQ*AEIVPLLSNLDNKSE
TPS*KWTRTGPDFGVMVRCPFHYTMFELRPPLS
FPRWLTQSLGEALDFRKSKSGSGTLSFLPWLVT
PPTHQKEKEGDSPLPQCESDPTPPRCGPGLLGSP
PQPWIMLLPHL*HAFVFVMSPPSQLSLWPCLLR
GTPGNISYSALGPTT*ATPPKEV*ELGGKEKRG
K*VFMGLNGEKRSSSILL*NESVK*TFVNVKLLR
YIIITEGEGAPKCKIFHKIPRDRKILWLANWKH
VGESKRGQQRRQECVADLVKARDMAAKGCKQCL
LNDFQREKKFARSLSSQPM*KALLMVIKMAHTYI
ALTLLQVLL*INALCKP

Sequence VDR.aAug05

GTGTGCGCCGGGAGCGCGGAACAGCTTGTCCACCCGCCGGCCGGACCAGGTGCGAACCCGGGAGCAGCGGGAAAGGGGGTCTCAGGATAGGGACTCGGGG
TCGGGGCGTCTGGGATACCGGGGCCTGAGCGCCCGGCTGCGAGCATTAGAGTCTAAGTCTCAGCGGTAAACTTGGCTACTGAGGTCCGGGCTGTCGTGCC
ATGAGGCTGGGACACTAAGGGGCACTGAGGTTTGAGAAAGCTGAAGTTCGTGCCAGGCTGGCGAGGGGAGCAGCGACATCCTCGGCGCTTAGGAGAAATG
CTCCGCTAACACAGTGCTTAGCACTTGGGCAACAAGAAGTATTTGTTTCCTTCTTCTGTCGGGGCGCCTTGGCATGGAGTGGAGGAATAAGAAAAGGAGC
GATTGGCTGTCGATGGTGCTCAGAACTGCTGGAGTGGAGG
AAGCCTTTGGGTCTGAAGTGTCTGTGAGACCTCACAGAAGAGCACCCCTGGGCTCCACTT
ACCTG
CCCCCTGCTCCTTCAGGGATGGAGGCAATGGCGGCCAGCACTTCCCTGCCTGACCCTGGAGACTTTGACCGGAACGTGCCCCGGATCTGTGGGGT
GTGTGGAGACCGAGCCACTGGCTTTCACTTCAATGCTATGACCTGTGAAGGCTGCAAAGGCTTCTTCAGGCGAAGCATGAAGCGGAAGGCACTATTCACC
TGCCCCTTCAACGGGGACTGCCGCATCACCAAGGACAACCGACGCCACTGCCAGGCCTGCCGGCTCAAACGCTGTGTGGACATCGGCATGATGAAGGAGT
TCATTCTGACAGATGAGGAAGTGCAGAGGAAGCGGGAGATGATCCTGAAGCGGAAGGAGGAGGAGGCCTTGAAGGACAGTCTGCGGCCCAAGCTGTCTGA
GGAGCAGCAGCGCATCATTGCCATACTGCTGGACGCCCACCATAAGACCTACGACCCCACCTACTCCGACTTCTGCCAGTTCCGGCCTCCAGTTCGTGTG
AATGATGGTGGAGGGAGCCATCCTTCCAGGCCCAACTCCAGACACACTCCCAGCTTCTCTGGGGACTCCTCCTCCTCCTGCTCAGATCACTGTATCACCT
CTTCAGACATGATGGACTCGTCCAGCTTCTCCAATCTGGATCTGAGTGAAGAAGATTCAGATGACCCTTCTGTGACCCTAGAGCTGTCCCAGCTCTCCAT
GCTGCCCCACCTGGCTGACCTGGTCAGTTACAGCATCCAAAAGGTCATTGGCTTTGCTAAGATGATACCAGGATTCAGAGACCTCACCTCTGAGGACCAG
ATCGTACTGCTGAAGTCAAGTGCCATTGAGGTCATCATGTTGCGCTCCAATGAGTCCTTCACCATGGACGACATGTCCTGGACCTGTGGCAACCAAGACT
ACAAGTACCGCGTCAGTGACGTGACCAAAGCCGGACACAGCCTGGAGCTGATTGAGCCCCTCATCAAGTTCCAGGTGGGACTGAAGAAGCTGAACTTGCA
TGAGGAGGAGCATGTCCTGCTCATGGCCATCTGCATCGTCTCCCCAGATCGTCCTGGGGTGCAGGACGCCGCGCTGATTGAGGCCATCCAGGACCGCCTG
TCCAACACACTGCAGACGTACATCCGCTGCCGCCACCCGCCCCCGGGCAGCCACCTGCTCTATGCCAAGATGATCCAGAAGCTAGCCGACCTGCGCAGCC
TCAATGAGGAGCACTCCAAGCAGTACCGCTGCCTCTCCTTCCAGCCTGAGTGCAGCATGAAGCTAACGCCCCTTGTGCTCGAAGTGTTTGGCAATGAGAT
CTCCTGACTAGGACAGCCTGTGGCGGTGCCTGGGTGGGGCTGCTCCTCCAGGGCCACGTGCCAGGCCCGGGGCTGGCGGCTACTCAGCAGCCCTCCTCAC
CCCGTCTGGGGTTCAGCCCCTCCTCTGCCACCTCCCCTATCCACCCAGCCCATTCTCTCTCCTGTCCAACCTAACCCCTTTCCTGCGGGCTTTTCCCCGG
TCCCTTGAGACCTCAGCCATGAGGAGTTGCTGTTTGTTTGACAAAGAAACCCAAGTGGGGGCAGAGGGCAGAGGCTGGAGGCAGGGCCTTGCCCAGAGAT
GCCTCCACCGCTGCCTAAGTGGCTGCTGACTGATGTTGAGGGAACAGACAGGAGAAATGCATCCATTCCTCAGGGACAGAGACACCTGCACCTCCCCCCA
CTGCAGGCCCCGCTTGTCCAGCGCCTAGTGGGGTCTCCCTCTCCTGCCTACTCACGATAAATAATCGGCCCACAGCTCCCACCCCACCCCCTTCAGTGCC
CACCAACATCCCATTGCCCTGGTTATATTCTCACGGGCAGTAGCTGTGGTGAGGTGGGTTTTCTTCCCATCACTGGAGCACCAGGCACGAACCCACCTGC
TGAGAGACCCAAGGAGGAAAAACAGACAAAAACAGCCTCACAGAAGAATATGACAGCTGTCCCTGTCACCAAGCTCACAGTTCCTCGCCCTGGGTCTAAG
GGGTTGGTTGAGGTGGAAGCCCTCCTTCCACGGATCCATGTAGCAGGACTGAATTGTCCCCAGTTTGCAGAAAAGCACCTGCCGACCTCGTCCTCCCCCT
GCCAGTGCCTTACCTCCTGCCCAGGAGAGCCAGCCCTCCCTGTCCTCCTCGGATCACCGAGAGTAGCCGAGAGCCTGCTCCCCCACCCCCTCCCCAGGGG
AGAGGGTCTGGAGAAGCAGTGAGCCGCATCTTCTCCATCTGGCAGGGTGGGATGGAGGAGAAGAATTTTCAGACCCCAGCGGCTGAGTCATGATCTCCCT
GCCGCCTCAATGTGGTTGCAAGGCCGCTGTTCACCCACAGGGCTAAGAGCTAGCGCTGCCGCACCCCAGAGTGTGGGAAGGGAGAGCGGGGCAGTCTCGG
GTGGCTAGTCAGAGAGAGTGTTTGGGGGTTCCGTGATGTAGGGTAAGGTGCCTTCTTATTCTCACTCCACCACCCAAAAGTCAAAAGGTGCCTGTGAGGC
AGGGGCGGAGTGATACAACTTCAAGTGCATGCTCTCTGCAGCCAGCCCAGCCCAGCTGGTGGGAAGCGTCTGTCCGTTTACTCCAAGGTGGGGTCTTTGT
GAGAGTGAGCTGTAGGTGTGCGGGACCGGTACAGAAAGGCGTTCTTCGAGGTGGATCACAGAGGCTTCTTCAGATCAGTGCTTGAGTTTGGGGAATGCGG
CCGCATTCCCTGAGTCACCAGGAATGTTAAAGTCAGTGGGAACGTGACTGCCCCAACTCCTGGAAGCTGTGTCCTTGCACCTGCATCCGTAGTTCCCTGA
AAACCCAGAGAGGAATCAGACTTCACACTGCAAGAGCCTTGGTGTCCACCTGGCCCCATGTCTCTCAGAATTCTTCAGGTGGAAAAACATCTGAAAGCCA
CGTTCCTTACTGCAGAATAGCATATATATCGCTTAATCTTAAATTTATTAGATATGAGTTGTTTTCAGACTCAGACTCCATTTGTATTATAGTCTAATAT
ACAGGGTAGCAGGTACCACTGATTTGGAGATATTTATGGGGGGAGAACTTACATTGTGAAACTTCTGTACATTAATTATTATTGCTGTTGTTATTTTACA
AGGGTCTAGGGAGAGACCCTTGTTTGATTTTAGCTGCAGAACGTATTGGTCCAGCTTGCTCTTCAGTGGGAGAAAACACTTGTAAGTTGCTAAACGAGTC
AATCCCCTCATTCAGGAAAACTGACAGAGGAGGGCGTGACTCACCCAAGCATATATAACTAGCTAGAAGTGGGCCAGGACAGGCCCGGCGCGGTGGCTCA
CGCCTGTAATCCCAGCAGTTTGGGAGGTCGAGGTAGGTGGATCACCTGAGGTCGGGAGTTCGAGACCAACCTGACCAACATGGAGAAACCCTGTCTCTAT
TAAAAATACAAAAAAAAAAAAAAAAAAAATAGCCGGGCATGGTGGCGCAAGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGAAGAATTGAACCCAG
GAGGTGGAGGTTGCAGTGAGCTGAGATCGTGCCGTTACTCTCCAACCTGGACAACAAGAGCGAAACTCCGTCTTAGAAGTGGACCAGGACAGGACCAGAT
TTTGGAGTCATGGTCCGGTGTCCTTTTCACTACACCATGTTTGAGCTCAGACCCCCACTCTCATTCCCCAGGTGGCTGACCCAGTCCCTGGGGGAAGCCC
TGGATTTCAGAAAGAGCAAGTCTGGATCTGGGACCCTTTCCTTCCTTCCCTGGCTTGTAACTCCACCAACCCATCAGAAGGAGAAGGAAGGAGACTCACC
TCTGCCTCAATGTGAATCAGACCCTACCCCACCACGATGTGGCCCTGGCCTGCTGGGCTCTCCACCTCAGCCTTGGATAATGCTGTTGCCTCATCTATAA
CATGCATTTGTCTTTGTAATGTCACCACCTTCCCAGCTCTCCCTCTGGCCCTGCCTTCTTCGGGGAACTCCTGGAAATATCAGTTACTCAGCCCTGGGCC
CCACCACCTAGGCCACTCCTCCAAAGGAAGTCTAGGAGCTGGGAGGAAAAGAAAAGAGGGGAAAATGAGTTTTTATGGGGCTGAACGGGGAGAAAAGGTC
ATCATCGATTCTACTTTAGAATGAGAGTGTGAAATAGACATTTGTAAATGTAAAACTTTTAAGGTATATCATTATAACTGAAGGAGAAGGTGCCCCAAAA
TGCAAGATTTTCCACAAGATTCCCAGAGACAGGAAAATCCTCTGGCTGGCTAACTGGAAGCATGTAGGAGAATCCAAGCGAGGTCAACAGAGAAGGCAGG
AATGTGTGGCAGATTTAGTGAAAGCTAGAGATATGGCAGCGAAAGGATGTAAACAGTGCCTGCTGAATGATTTCCAAAGAGAAAAAAAGTTTGCCAGAAG
TTTGTCAAGTCAACCAATGTAGAAAGCTTTGCTTATGGTAATAAAAATGGCTCATACTTATATAGCACTTACTTTGTTGCAAGTACTGCTGTAAATAAAT
GCTTTATGCAAACCAA
  • VDR.F2 (forward primer)
  • VDR.R2 (reverse primer)
  • Alternative donor

Frame 0

VCAGSAEQLVHPPAGPGANPGAAGKGVSG*GLG
VGASGIPGPERPAASIRV*VSAVNLATEVRAVVP
*GWDTKGH*GLRKLKFVPGWRGEQRHPRRLGEM
LR*HSA*HLGNKKYLFPSSVGAPWHGVEE*EKE
RLAVDGAQNCWSGG
SLWV*SVCETSQKSTPGLHL
P
APCSFRDGGNGGQHFPA*PWRL*PERAPDLWG
VWRPSHWLSLQCYDL*RLQRLLQAKHEAEGTIH
LPLQRGLPHHQGQPTPLPGLPAQTLCGHRHDEGV
HSDR*GSAEEAGDDPEAEGGGGLEGQSAAQAV*
GAAAHHCHTAGRPP*DLRPHLLRLLPVPASSSC
E*WWREPSFQAQLQTHSQLLWGLLLLLLRSLYHL
FRHDGLVQLLQSGSE*RRFR*PFCDPRAVPALH
AAPPG*PGQLQHPKGHWLC*DDTRIQRPHL*GP
DRTAEVKCH*GHHVALQ*VLHHGRHVLDLWQPRL
QVPRQ*RDQSRTQPGAD*APHQVPGGTEEAELA
*GGACPAHGHLHRLPRSSWGAGRRAD*GHPGPP
VQHTADVHPLPPPAPGQPPALCQDDPEASRPAQP
Q*GALQAVPLPLLPA*VQHEANAPCARSVWQ*D
LLTRTACGGAWVGLLLQGHVPGPGLAATQQPSS
PRLGFSPSSATSPIHPAHSLSCPT*PLSCGLFPG
PLRPQP*GVAVCLTKKPKWGQRAEAGGRALPRD
ASTAA*VAAD*C*GNRQEKCIHSSGTETPAPPP
TAGPACPAPSGVSLSCLLTINNRPTAPTPPPSVP
TNIPLPWLYSHGQ*LW*GGFSSHHWSTRHEPTC
*ETQGGKTDKNSLTEEYDSCPCHQAHSSSPWV*
GVG*GGSPPSTDPCSRTELSPVCRKAPADLVLPL
PVPYLLPRRASPPCPPRITESSREPAPPPPPQG
RGSGEAVSRIFSIWQGGMEEKNFQTPAAES*SP
CRLNVVARPLFTHRAKS*RCRTPECGKGERGSLG
WLVRESVWGFRDVG*GAFLFSLHHPKVKRCL*G
RGGVIQLQVHALCSQPSPAGGKRLSVYSKVGSL
*E*AVGVRDRYRKAFFEVDHRGFFRSVLEFGECG
RIP*VTRNVKVSGNVTAPTPGSCVLAPASVVP*
KPREESDFTLQEPWCPPGPMSLRILQVEKHLKA
TFLTAE*HIYRLILNLLDMSCFQTQTPFVL*SNI
QGSRYH*FGDIYGGRTYIVKLLYINYYCCCYFT
RV*GETLV*F*LQNVLVQLALQWEKTLVSC*TS
QSPHSGKLTEEGVTHPSIYN*LEVGQDRPGAVAH
ACNPSSLGGRGRWIT*GREFETNLTNMEKPCLY
*KYKKKKKKNSRAWWRKPVIPATQEAEAEELNP
GGGGCSELRSCRYSPTWTTRAKLRLRSGPGQDQI
LESWSGVLFTTPCLSSDPHSHSPGG*PSPWGKP
WISERASLDLGPFPSFPGL*LHQPIRRRRKETH
LCLNVNQTLPHHDVALACWALHLSLG*CCCLIYN
MHLSL*CHHLPSSPSGPAFFGELLEISVTQPWA
PPPRPLLQRKSRSWEEKKRGENEFLWG*TGRKG
HHRFYFRMRV*NRHL*M*NF*GISL*LKEKVPQN
ARFSTRFPETGKSSGWLTGSM*ENPSEVNREGR
NVWQI**KLEIWQRKDVNSAC*MISKEKKSLPE
VCQVNQCRKLCLW**KWLILI*HLLCCKYCCK*M
LYANQ

Frame 1

CAPGARNSLSTRRPDQVRTREQRERGSQDRDSG
SGRLGYRGLSARLRALESKSQR*TWLLRSGLSC
HEAGTLRGTEV*ES*SSCQAGEGSSDILGA*EKC
SANTVLSTWATRSICFLLLSGRLGMEWRNKKRS
DWLSMVLRTAGVE
EAFGSEVSVRPHRRAPLGST
YL
PPAPSGMEAMAASTSLPDPGDFDRNVPRICGV
CGDRATGFHFNAMTCEGCKGFFRRSMKRKALFT
CPFNGDCRITKDNRRHCQACRLKRCVDIGMMKE
FILTDEEVQRKREMILKRKEEEALKDSLRPKLSE
EQQRIIAILLDAHHKTYDPTYSDFCQFRPPVRV
NDGGGSHPSRPNSRHTPSFSGDSSSSCSDHCIT
SSDMMDSSSFSNLDLSEEDSDDPSVTLELSQLSM
LPHLADLVSYSIQKVIGFAKMIPGFRDLTSEDQ
IVLLKSSAIEVIMLRSNESFTMDDMSWTCGNQD
YKYRVSDVTKAGHSLELIEPLIKFQVGLKKLNLH
EEEHVLLMAICIVSPDRPGVQDAALIEAIQDRL
SNTLQTYIRCRHPPPGSHLLYAKMIQKLADLRS
LNEEHSKQYRCLSFQPECSMKLTPLVLEVFGNEI
S*LGQPVAVPGWGCSSRATCQARGWRLLSSPPH
PVWGSAPPLPPPLSTQPILSPVQPNPFPAGFSP
VP*DLSHEELLFV*QRNPSGGRGQRLEAGPCPEM
PPPLPKWLLTDVEGTDRRNASIPQGQRHLHLPP
LQAPLVQRLVGSPSPAYSR*IIGPQLPPHPLQC
PPTSHCPGYILTGSSCGEVGFLPITGAPGTNPPA
ERPKEEKQTKTASQKNMTAVPVTKLTVPRPGSK
GLVEVEALLPRIHVAGLNCPQFAEKHLPTSSSP
CQCLTSCPGEPALPVLLGSPRVAESLLPHPLPRG
EGLEKQ*AASSPSGRVGWRRRIFRPQRLSHDLP
AASMWLQGRCSPTGLRASAAAPQSVGRESGAVS
GG*SERVFGGSVM*GKVPSYSHSTTQKSKGACEA
GAE*YNFKCMLSAASPAQLVGSVCPFTPRWGLC
ESEL*VCGTGTERRSSRWITEASSDQCLSLGNA
AAFPESPGMLKSVGT*LPQLLEAVSLHLHP*FPE
NPERNQTSHCKSLGVHLAPCLSEFFRWKNI*KP
RSLLQNSIYIA*S*IY*I*VVFRLRLHLYYSLI
YRVAGTTDLEIFMGGELTL*NFCTLIIIAVVILQ
GSRERPLFDFSCRTYWSSLLFSGRKHL*VAKRV
NPLIQEN*QRRA*LTQAYITS*KWARTGPARWL
TPVIPAVWEVEVGGSPEVGSSRPT*PTWRNPVSI
KNTKKKKKKIAGHGGASL*SQLLRRLRQKN*TQ
EVEVAVS*DRAVTLQPGQQERNSVLEVDQDRTR
FWSHGPVSFSLHHV*AQTPTLIPQVADPVPGGSP
GFQKEQVWIWDPFLPSLACNSTNPSEGEGRRLT
SASM*IRPYPTTMWPWPAGLSTSALDNAVASSI
TCICLCNVTTFPALPLALPSSGNSWKYQLLSPGP
HHLGHSSKGSLGAGRKRKEGKMSFYGAERGEKV
IIDSTLE*ECEIDICKCKTFKVYHYN*RRRCPK
MQDFPQDSQRQENPLAG*LEACRRIQARSTEKAG
MCGRFSES*RYGSERM*TVPAE*FPKRKKVCQK
FVKSTNVESFAYGNKNGSYLYSTYFVASTAVNK
CFMQT

Frame 2

VRRERGTACPPAGRTRCEPGSSGKGGLRIGTRG
RGVWDTGA*APGCEH*SLSLSGKLGY*GPGCRA
MRLGH*GALRFEKAEVRARLARGAATSSALRRN
APLTQCLALGQQEVFVSFFCRGALAWSGGIRKGA
IGCRWCSELLEWR
KPLGLKCL*DLTEEHPWAPL
TC
PLLLQGWRQWRPALPCLTLETLTGTCPGSVG
CVETEPLAFTSML*PVKAAKASSGEA*SGRHYSP
APSTGTAASPRTTDATARPAGSNAVWTSA**RS
SF*QMRKCRGSGR*S*SGRRRRP*RTVCGPSCL
RSSSASLPYCWTPTIRPTTPPTPTSASSGLQFV*
MMVEGAILPGPTPDTLPASLGTPPPPAQITVSP
LQT*WTRPASPIWI*VKKIQMTLL*P*SCPSSP
CCPTWLTWSVTASKRSLALLR*YQDSETSPLRTR
SYC*SQVPLRSSCCAPMSPSPWTTCPGPVATKT
TSTASVT*PKPDTAWS*LSPSSSSRWD*RS*TC
MRRSMSCSWPSASSPQIVLGCRTPR*LRPSRTAC
PTHCRRTSAAATRPRAATCSMPR*SRS*PTCAA
SMRSTPSSTAASPSSLSAA*S*RPLCSKCLAMR
SPD*DSLWRCLGGAAPPGPRARPGAGGYSAALLT
PSGVQPLLCHLPYPPSPFSLLSNLTPFLRAFPR
SLETSAMRSCCLFDKETQVGAEGRGWRQGLAQR
CLHRCLSGC*LMLREQTGEMHPFLRDRDTCTSPH
CRPRLSSA*WGLPLLPTHDK*SAHSSHPTPFSA
HQHPIALVIFSRAVAVVRWVFFPSLEHQARTHL
LRDPRRKNRQKQPHRRI*QLSLSPSSQFLALGLR
GWLRWKPSFHGSM*QD*IVPSLQKSTCRPRPPP
ASALPPAQESQPSLSSSDHRE*PRACSPTPSPG
ERVWRSSEPHLLHLAGWDGGEEFSDPSG*VMISL
PPQCGCKAAVHPQG*ELALPHPRVWEGRAGQSR
VASQRECLGVP*CRVRCLLILTPPPKSQKVPVR
QGRSDTTSSACSLQPAQPSWWEASVRLLQGGVFV
RVSCRCAGPVQKGVLRGGSQRLLQISA*VWGMR
PHSLSHQEC*SQWERDCPNSWKLCPCTCIRSSL
KTQRGIRLHTARALVSTWPHVSQNSSGGKTSESH
VPYCRIAYISLNLKFIRYELFSDSDSICIIV*Y
TG*QVPLIWRYLWGENLHCETSVH*LLLLLLFY
KGLGRDPCLILAAERIGPACSSVGENTCKLLNES
IPSFRKTDRGGRDSPKHI*LARSGPGQARRGGS
RL*SQQFGRSR*VDHLRSGVRDQPDQHGETLSL
LKIQKKKKKK*PGMVAQACNPSYSGG*GRRIEPR
RWRLQ*AEIVPLLSNLDNKSETPS*KWTRTGPD
FGVMVRCPFHYTMFELRPPLSFPRWLTQSLGEA
LDFRKSKSGSGTLSFLPWLVTPPTHQKEKEGDSP
LPQCESDPTPPRCGPGLLGSPPQPWIMLLPHL*
HAFVFVMSPPSQLSLWPCLLRGTPGNISYSALG
PTT*ATPPKEV*ELGGKEKRGK*VFMGLNGEKRS
SSILL*NESVK*TFVNVKLLRYIIITEGEGAPK
CKIFHKIPRDRKILWLANWKHVGESKRGQQRRQ
ECVADLVKARDMAAKGCKQCLLNDFQREKKFARS
LSSQPM*KALLMVIKMAHTYIALTLLQVLL*IN
ALCKP

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