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Sequences

Sequence TP73L.gAug05

AGAGAGAAAGAGAGAGAGGGACTTGAGTTCTGTTATCTTCTTAAGTAGATTCATATTGTAAGGGTCTCGGGGTGGGGGGGTTGGCAAAATCCTGGAGCCA GAAGAAAGGACAGCAGCATTGATCAATCTTACAGCTAACATGTTGTACCTGGAAAACAATGCCCAGACTCAATTTAGTGAGCCACAGTACACGAACCTGG GGCTCCTGAACAGCATGGACCAGCAGATTCAGAACGGCTCCTCGTCCACCAGTCCCTATAACACAGACCACGCGCAGAACAGCGTCACGGCGCCCTCGCC CTACGCACAGCCCAGCTCCACCTTCGATGCTCTCTCTCCATCACCCGCCATCCCCTCCAACACCGACTACCCAGGCCCGCACAGTTTCGACGTGTCCTTC CAGCAGTCGAGCACCGCCAAGTCGGCCACCTGGACGTATTCCACTGAACTGAAGAAACTCTACTGCCAAATTGCAAAGACATGCCCCATCCAGATCAAGG TGATGACCCCACCTCCTCAGGGAGCTGTTATCCGCGCCATGCCTGTCTACAAAAAAGCTGAGCACGTCACGGAGGTGGTGAAGCGGTGCCCCAACCATGA GCTGAGCCGTGAATTCAACGAGGGACAGATTGCCCCTCCTAGTCATTTGATTCGAGTAGAGGGGAACAGCCATGCCCAGTATGTAGAAGATCCCATCACA GGAAGACAGAGTGTGCTGGTACCTTATGAGCCACCCCAGGTTGGCACTGAATTCACGACAGTCTTGTACAATTTCATGTGTAACAGCAGTTGTGTTGGAG GGATGAACCGCCGTCCAATTTTAATCATTGTTACTCTGGAAACCAGAGATGGGCAAGTCCTGGGCCGACGCTGCTTTGAGGCCCGGATCTGTGCTTGCCC AGGAAGAGACAGGAAGGCGGATGAAGATAGCATCAGAAAGCAGCAAGTTTCGGACAGTACAAAGAACGGTGATGGTACGAAGCGCCCGTTTCGTCAGAAC ACACATGGTATCCAGATGACATCCATCAAGAAACGAAGATCCCCAGATGATGAACTGTTATACTTACCAGTGAGGGGCCGTGAGACTTATGAAATGCTGT TGAAGATCAAAGAGTCCCTGGAACTCATGCAGTACCTTCCTCAGCACACAATTGAAACGTACAGGCAACAGCAACAGCAGCAGCACCAGCACTTACTTCA GAAACAGACCTCAATACAGTCTCCATCTTCATATGGTAACAGCTCCCCACCTCTGAACAAAATGAACAGCATGAACAAGCTGCCTTCTGTGAGCCAGCTT ATCAACCCTCAGCAGCGCAACGCCCTCACTCCTACAACCATTCCTGATGGCATGGGAGCCAACATTCCCATGATGGGCACCCACATGCCAATGGCTGGAG ACATGAATGGACTCAGCCCCACCCAGGCACTCCCTCCCCCACTCTCCATGCCATCCACCTCCCACTGCACACCCCCACCTCCGTATCCCACAGATTGCAG CATTGTCAGGATCTGGCAAGTCTGAAAATCCCTGAGCAATTTCGACATGCGATCTGGAAGGGCATCCTGGACCACCGGCAGCTCCACGAATTCTCCTCCC CTTCTCATCTCCTGCGGACCCCAAGCAGTGCCTCTACAGTCAGTGTGGGCTCCAGTGAGACCCGGGGTGAGCGTGTTATTGATGCTGTGCGATTCACCCT CCGCCAGACCATCTCTTTCCCACCCCGAGATGAGTGGAATGACTTCAACTTTGACATGGATGCTCGCCGCAATAAGCAACAGCGCATCAAAGAGGAGGGG GAGTGAGCCTCACCATGTGAGCTCTTCCTATCCCTCTCCTAACTGCCAGCCCCCTAAAAGCACTCCTGCTTAATCTTCAAAGCCTTCTCCCTAGCTCCTC CCCTTCCTCTTGTCTGATTTCTTAGGGGAAGGAGAAGTAAGAGGCTACCTCTTACCTAACATCTGACCTGGCATCTAATTCTGATTCTGGCTTTAAGCCT TCAAAACTATAGCTTGCAGAACTGTAGCTGCCATGGCTAGGTAGAAGTGAGCAAAAAAGAGTTGGGTGTCTCCTTAAGCTGCAGAGATTTCTCATTGACT TTTATAAAGCATGTTCACCCTTATAGTCTAAGACTATATATATAAATGTATAAATATACAGTATAGATTTTTGGGTGGGGGGCATTGAGTATTGTTTAAA ATGTAATTTAAATGAAAGAAAATTGAGTTGCACTTATTGACCATTTTTTAATTTACTTGTTTTGGATGGCTTGTCTATACTCCTTCCCTTAAGGGGTATC ATGTATGGTGATAGGTATCTAGAGCTTAATGCTACATGTGAGTGACGATGATGTACAGATTCTTTCAGTTCTTTGGATTCTAAATACATGCCACATCAAA CCTTTGAGTAGATCCATTTCCATTGCTTATTATGTAGGTAAGACTGTAGATATGTATTCTTTTCTCAGTGTTGGTATATTTTATATTACTGACATTTCTT CTAGTGATGATGGTTCACGTTGGGGTGATTTAATCCAGTTATAAGAAGAAGTTCATGTCCAAACGTCCTCTTTAGTTTTTGGTTGGGAATGAGGAAAATT CTTAAAAGGCCCATAGCAGCCAGTTCAAAAACACCCGACGTCATGTATTTGAGCATATCAGTAACCCCCTTAAATTTAATACCAGATACCTTATCTTACA ATATTGATTGGGAAAACATTTGCTGCCATTACAGAGGTATTAAAACTAAATTTCACTACTAGATTGACTAACTCAAATACACATTTGCTACTGTTGTAAG AATTCTGATTGATTTGATTGGGATGAATGCCATCTATCTAGTTCTAACAGTGAAGTTTTACTGTCTATTAATATTCAGGGTAAATAGGAATCATTCAGAA ATGTTGAGTCTGTACTAAACAGTAAGATATCTCAATGAACCATAAATTCAACTTTGTAAAAATCTTTTGAAGCATAGATAATATTGTTTGGTAAATGTTT CTTTTGTTTGGTAAATGTTTCTTTTAAAGACCCTCCTATTCTATAAAACTCTGCATGTAGAGGCTTGTTTACCTTTCTCTCTCTAAGGTTTACAATAGGA GTGGTGATTTGAAAAATATAAAATTATGAGATTGGTTTTCCTGTGGCATAAATTGCATCACTGTATCATTTTCTTTTTTAACCGGTAAGAGTTTCAGTTT GTTGGAAAGTAACTGTGAGAACCCAGTTTCCCGTCCATCTCCCTTAGGGACTACCCATAGACATGAAAGGTCCCCACAGAGCAAGAGATAAGTCTTTCAT GGCTGCTGTTGCTTAAACCACTTAAACGAAGAGTTCCCTTGAAACTTTGGGAAAACATGTTAATGACAATATTCCAGATCTTTCAGAAATATAACACATT TTTTTGCATGCATGCAAATGAGCTCTGAAATCTTCCCATGCATTCTGGTCAAGGGCTGTCATTGCACATAAGCTTCCATTTTAATTTTAAAGTGCAAAAG GGCCAGCGTGGCTCTAAAAGGTAATGTGTGGATTGCCTCTGAAAAGTGTGTATATATTTTGTGTGAAATTGCATACTTTGTATTTTGATTATTTTTTTTT TCTTCTTGGGATAGTGGGATTTCCAGAACCACACTTGAAACCTTTTTTTATCGTTTTTGTATTTTCATGAAAATACCATTTAGTAAGAATACCACATCAA ATAAGAAATAATGCTACAATTTTAAGAGGGGAGGGAAGGGAAAGTTTTTTTTTATTATTTTTTTAAAATTTTGTATGTTAAAGAGAATGAGTCCTTGATT TCAAAGTTTTGTTGTACTTAAATGGTAATAAGCACTGTAAACTTCTGCAACAAGCATGCAGCTTTGCAAACCCATTAAGGGGAAGAATGAAAGCTGTTCC TTGGTCCTAGTAAGAAGACAAACTGCTTCCCTTACTTTGCTGAGGGTTTGAATAAACCTAGGACTTCCGAGCTATGTCAGTACTATTCAGGTAACACTAG GGCCTTGGAAATTCCTGTACTGTGTCTCATGGATTTGGCACTAGCCAAAGCGAGGCACCCTTACTGGCTTACCTCCTCATGGCAGCCTACTCTCCTTGAG TGTATGAGTAGCCAGGGTAAGGGGTAAAAGGATAGTAAGCATAGAAACCACTAGAAAGTGGGCTTAATGGAGTTCTTGTGGCCTCAGCTCAATGCAGTTA GCTGAAGAATTGAAAAGTTTTTGTTTGGAGACGTTTATAAACAGAAATGGAAAGCAGAGTTTTCATTAAATCCTTTTACCTTTTTTTTTTCTTGGTAATC CCCTAAAATAACAGTATGTGGGATATTGAATGTTAAAGGGATATTTTTTTCTATTATTTTTATAATTGTACAAAATTAAGCAAATGTTAAAAGTTTTATA TGCTTTATTAATGTTTTCAAAAGGTATTATACATGTGATACATTTTTTAAGCTTCAGTTGCTTGTCTTCTGGTACTTTCTGTTATGGGCTTTTGGGGAGC CAGAAGCCAATCTACAATCTCTTTTTGTTTGCCAGGACATGCAATAAAATTTAAAAAATAAATAAAAACTAATTAAGAAATTGTGTGTGTGTCTATCTGT GTCTGTTTGCCTATTTGTCCAT

TP73L.F10 (forward primer)
TP73L.R8 (reverse primer)
Single exon skipping

Frame 0

REKEREGLEFCYLLK*IHIVRVSGWGGWQNPGA RRKDSSIDQSYS*HVVPGKQCPDSI**ATVHEPG APEQHGPADSERLLVHQSL*HRPRAEQRHGALA LRTAQLHLRCSLSITRHPLQHRLPRPAQFRRVL PAVEHRQVGHLDVFH*TEETLLPNCKDMPHPDQG DDPTSSGSCYPRHACLQKS*ARHGGGEAVPQP* AEP*IQRGTDCPS*SFDSSRGEQPCPVCRRSHH RKTECAGTL*ATPGWH*IHDSLVQFHV*QQLCWR DEPPSNFNHCYSGNQRWASPGPTLL*GPDLCLP RKRQEGG*R*HQKAASFGQYKER*WYEAPVSSE HTWYPDDIHQETKIPR**TVILTSEGP*DL*NAV EDQRVPGTHAVPSSAHN*NVQATATAAAPALTS ETDLNTVSIFIW*QLPTSEQNEQHEQAAFCEPA YQPSAAQRPHSYNHS*WHGSQHSHDGHPHANGWR HEWTQPHPGTPSPTLHAIHLPLHTPTSVSHRLQ HCQDLASLKIPEQFRHAIWKGILDHRQLHEFSS PSHLLRTPSSASTVSVGSSETRGERVIDAVRFTL RQTISFPPRDEWNDFNFDMDARRNKQQRIKEEG E*ASPCELFLSLS*LPAP*KHSCLIFKAFSLAP PLPLV*FLRGRRSKRLPLT*HLTWHLILILALSL QNYSLQNCSCHG*VEVSKKELGVSLSCRDFSLT FIKHVHPYSLRLYI*MYKYTV*IFGWGALSIV* NVI*MKEN*VALIDHFLIYLFWMACLYSFP*GVS CMVIGI*SLMLHVSDDDVQILSVLWILNTCHIK PLSRSISIAYYVGKTVDMYSFLSVGIFYITDIS SSDDGSRWGDLIQL*EEVHVQTSSLVFGWE*GKF LKGP*QPVQKHPTSCI*AYQ*PP*I*YQIPYLT ILIGKTFAAITEVLKLNFTTRLTNSNTHLLLL* EF*LI*LG*MPSI*F*Q*SFTVY*YSG*IGIIQK C*VCTKQ*DISMNHKFNFVKIF*SIDNIVW*MF LLFGKCFF*RPSYSIKLCM*RLVYLSLSKVYNR SGDLKNIKL*DWFSCGINCITVSFSFLTGKSFSL LESNCENPVSRPSPLGTTHRHERSPQSKR*VFH GCCCLNHLNEEFP*NFGKTC**QYSRSFRNITH FFACMQMSSEIFPCILVKGCHCT*ASILILKCKR ASVALKGNVWIASEKCVYILCEIAYFVF*LFFF SSWDSGISRTTLETFFYRFCIFMKIPFSKNTTS NKK*CYNFKRGGKGKFFFIIFLKFCMLKRMSP*F QSFVVLKW**AL*TSATSMQLCKPIKGKNESCS LVLVRRQTASLTLLRV*INLGLPSYVSTIQVTL GPWKFLYCVSWIWH*PKRGTLTGLPPHGSLLSLS V*VARVRGKRIVSIETTRKWA*WSSCGLSSMQL AEELKSFCLETFINRNGKQSFH*ILLPFFFLGN PLK*QYVGY*MLKGYFFLLFL*LYKIKQMLKVLY ALLMFSKGIIHVIHFLSFSCLSSGTFCYGLLGS QKPIYNLFLFARTCNKI*KINKN*LRNCVCVYL CLFAYLS

Frame 1

ERKRERDLSSVIFLSRFIL*GSRGGGVGKILEP EERTAALINLTANMLYLENNAQTQFSEPQYTNL GLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSP YAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSF QQSSTAKSATWTYSTELKKLYCQIAKTCPIQIK VMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHE LSREFNEGQIAPPSHLIRVEGNSHAQYVEDPIT GRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVG GMNRRPILIIVTLETRDGQVLGRRCFEARICACP GRDRKADEDSIRKQQVSDSTKNGDGTKRPFRQN THGIQMTSIKKRRSPDDELLYLPVRGRETYEML LKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQ KQTSIQSPSSYGNSSPPLNKMNSMNKLPSVSQL INPQQRNALTPTTIPDGMGANIPMMGTHMPMAG DMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCS IVRIWQV*KSLSNFDMRSGRASWTTGSSTNSPP LLISCGPQAVPLQSVWAPVRPGVSVLLMLCDSP SARPSLSHPEMSGMTSTLTWMLAAISNSASKRRG SEPHHVSSSYPSPNCQPPKSTPA*SSKPSP*LL PFLLSDFLGEGEVRGYLLPNI*PGI*F*FWL*A FKTIACRTVAAMAR*K*AKKSWVSP*AAEISH*L L*SMFTLIV*DYIYKCINIQYRFLGGGH*VLFK M*FK*KKIELHLLTIF*FTCFGWLVYTPSLKGY HVW**VSRA*CYM*VTMMYRFFQFFGF*IHATSN L*VDPFPLLIM*VRL*ICILFSVLVYFILLTFL LVMMVHVGVI*SSYKKKFMSKRPL*FLVGNEEN S*KAHSSQFKNTRRHVFEHISNPLKFNTRYLILQ Y*LGKHLLPLQRY*N*ISLLD*LTQIHICYCCK NSD*FDWDECHLSSSNSEVLLSINIQGK*ESFR NVESVLNSKISQ*TINSTL*KSFEA*IILFGKCF FCLVNVSFKDPPIL*NSACRGLFTFLSLRFTIG VVI*KI*NYEIGFPVA*IASLYHFLF*PVRVSV CWKVTVRTQFPVHLP*GLPIDMKGPHRARDKSFM AAVA*TT*TKSSLETLGKHVNDNIPDLSEI*HI FLHACK*ALKSSHAFWSRAVIAHKLPF*F*SAK GPAWL*KVMCGLPLKSVYIFCVKLHTLYFDYFFF LLGIVGFPEPHLKPFFIVFVFS*KYHLVRIPHQ IRNNATILRGEGRESFFLLFF*NFVC*RE*VLD FKVLLYLNGNKHCKLLQQACSFANPLRGRMKAVP WS**EDKLLPLLC*GFE*T*DFRAMSVLFR*H* GLGNSCTVSHGFGTSQSEAPLLAYLLMAAYSP* VYE*PG*GVKG**A*KPLESGLNGVLVASAQCS* LKN*KVFVWRRL*TEMESRVFIKSFYLFFFLVI P*NNSMWDIEC*RDIFFYYFYNCTKLSKC*KFY MLY*CFQKVLYM*YIF*ASVACLLVLSVMGFWGA RSQSTISFCLPGHAIKFKK*IKTN*EIVCVSIC VCLPICP

Frame 2

RERERGT*VLLSS*VDSYCKGLGVGGLAKSWSQ KKGQQH*SILQLTCCTWKTMPRLNLVSHSTRTW GS*TAWTSRFRTAPRPPVPITQTTRRTASRRPR PTHSPAPPSMLSLHHPPSPPTPTTQARTVSTCPS SSRAPPSRPPGRIPLN*RNSTAKLQRHAPSRSR **PHLLRELLSAPCLSTKKLSTSRRW*SGAPTM S*AVNSTRDRLPLLVI*FE*RGTAMPSM*KIPSQ EDRVCWYLMSHPRLALNSRQSCTISCVTAVVLE G*TAVQF*SLLLWKPEMGKSWADAALRPGSVLA QEETGRRMKIASESSKFRTVQRTVMVRSARFVRT HMVSR*HPSRNEDPQMMNCYTYQ*GAVRLMKCC *RSKSPWNSCSTFLSTQLKRTGNSNSSSTSTYF RNRPQYSLHLHMVTAPHL*TK*TA*TSCLL*ASL STLSSATPSLLQPFLMAWEPTFP*WAPTCQWLE T*MDSAPPRHSLPHSPCHPPPTAHPHLRIPQIA ALSGSGKSENP*AISTCDLEGHPGPPAAPRILLP FSSPADPKQCLYSQCGLQ*DPG*ACY*CCAIHP PPDHLFPTPR*VE*LQL*HGCSPQ*ATAHQRGG GVSLTM*ALPIPLLTASPLKALLLNLQSLLPSSS PSSCLIS*GKEK*EATSYLTSDLASNSDSGFKP SKL*LAEL*LPWLGRSEQKRVGCLLKLQRFLID FYKACSPL*SKTIYINV*IYSIDFWVGGIEYCLK CNLNERKLSCTY*PFFNLLVLDGLSILLPLRGI MYGDRYLELNATCE*R*CTDSFSSLDSKYMPHQ TFE*IHFHCLLCR*DCRYVFFSQCWYILYY*HFF ***WFTLG*FNPVIRRSSCPNVLFSFWLGMRKI LKRPIAASSKTPDVMYLSISVTPLNLIPDTLSY NIDWENICCHYRGIKTKFHY*ID*LKYTFATVVR ILIDLIGMNAIYLVLTVKFYCLLIFRVNRNHSE MLSLY*TVRYLNEP*IQLCKNLLKHR*YCLVNV SFVW*MFLLKTLLFYKTLHVEACLPFSL*GLQ*E W*FEKYKIMRLVFLWHKLHHCIIFFFNR*EFQF VGK*L*EPSFPSISLRDYP*T*KVPTEQEISLS WLLLLKPLKRRVPLKLWENMLMTIFQIFQKYNTF FCMHANEL*NLPMHSGQGLSLHISFHFNFKVQK GQRGSKR*CVDCL*KVCIYFV*NCILCILIIFF FFLG*WDFQNHT*NLFLSFLYFHENTI**EYHIK *EIMLQF*EGREGKVFFYYFFKILYVKENESLI SKFCCT*MVISTVNFCNKHAALQTH*GEE*KLF LGPSKKTNCFPYFAEGLNKPRTSELCQYYSGNTR ALEIPVLCLMDLALAKARHPYWLTSSWQPTLLE CMSSQGKG*KDSKHRNH*KVGLMEFLWPQLNAV S*RIEKFLFGDVYKQKWKAEFSLNPFTFFFSW*S PKITVCGILNVKGIFFSIIFIIVQN*ANVKSFI CFINVFKRYYTCDTFFKLQLLVFWYFLLWAFGE PEANLQSLFVCQDMQ*NLKNK*KLIKKLCVCLSV SVCLFVH

Sequence TP73L.cAug05

TTTCGTAGAAACCCCAGCTCATTTCTCTTGGAAAGAAAGTTATTACCGATCCACCATGTCCCAGAGCACACAGACAAATGAATTCCTCAGTCCAGAGGTT TTCCAGCATATCTGGGATTTTCTGGAACAGCCTATATGTTCAGTTCAGCCCATTGACTTGAACTTTGTGGATGAACCATCAGAAGATGGTGCGACAAACA AGATTGAGATTAGCATGGACTGTATCCGCATGCAGGACTCGGACCTGAGTGACCCCATGTGGCCACAGTACACGAACCTGGGGCTCCTGAACAGCATGGA CCAGCAGATTCAGAACGGCTCCTCGTCCACCAGTCCCTATAACACAGACCACGCGCAGAACAGCGTCACGGCGCCCTCGCCCTACGCACAGCCCAGCTCC ACCTTCGATGCTCTCTCTCCATCACCCGCCATCCCCTCCAACACCGACTACCCAGGCCCGCACAGTTTCGACGTGTCCTTCCAGCAGTCGAGCACCGCCA AGTCGGCCACCTGGACGTATTCCACTGAACTGAAGAAACTCTACTGCCAAATTGCAAAGACATGCCCCATCCAGATCAAGGTGATGACCCCACCTCCTCA GGGAGCTGTTATCCGCGCCATGCCTGTCTACAAAAAAGCTGAGCACGTCACGGAGGTGGTGAAGCGGTGCCCCAACCATGAGCTGAGCCGTGAATTCAAC GAGGGACAGATTGCCCCTCCTAGTCATTTGATTCGAGTAGAGGGGAACAGCCATGCCCAGTATGTAGAAGATCCCATCACAGGAAGACAGAGTGTGCTGG TACCTTATGAGCCACCCCAGGTTGGCACTGAATTCACGACAGTCTTGTACAATTTCATGTGTAACAGCAGTTGTGTTGGAGGGATGAACCGCCGTCCAAT TTTAATCATTGTTACTCTGGAAACCAGAGATGGGCAAGTCCTGGGCCGACGCTGCTTTGAGGCCCGGATCTGTGCTTGCCCAGGAAGAGACAGGAAGGCG GATGAAGATAGCATCAGAAAGCAGCAAGTTTCGGACAGTACAAAGAACGGTGATGGTACGAAGCGCCCGTTTCGTCAGAACACACATGGTATCCAGATGA CATCCATCAAGAAACGAAGATCCCCAGATGATGAACTGTTATACTTACCAGTGAGGGGCCGTGAGACTTATGAAATGCTGTTGAAGATCAAAGAGTCCCT GGAACTCATGCAGTACCTTCCTCAGCACACAATTGAAACGTACAGGCAACAGCAACAGCAGCAGCACCAGCACTTACTTCAGAAACAGACCTCAATACAG TCTCCATCTTCATATGGTAACAGCTCCCCACCTCTGAACAAAATGAACAGCATGAACAAGCTGCCTTCTGTGAGCCAGCTTATCAACCCTCAGCAGCGCA ACGCCCTCACTCCTACAACCATTCCTGATGGCATGGGAGCCAACATTCCCATGATGGGCACCCACATGCCAATGGCTGGAGACATGAATGGACTCAGCCC CACCCAGGCACTCCCTCCCCCACTCTCCATGCCATCCACCTCCCACTGCACACCCCCACCTCCGTATCCCACAGATTGCAGCATTGTCAGGATCTGGCAA GTCTGAAAATCCCTGAGCAATTTCGACATGCGATCTGGAAGGGCATCCTGGACCACCGGCAGCTCCACGAATTCTCCTCCCCTTCTCATCTCCTGCGGAC CCCAAGCAGTGCCTCTACAGTCAGTGTGGGCTCCAGTGAGACCCGGGGTGAGCGTGTTATTGATGCTGTGCGATTCACCCTCCGCCAGACCATCTCTTTC CCACCCCGAGATGAGTGGAATGACTTCAACTTTGACATGGATGCTCGCCGCAATAAGCAACAGCGCATCAAAGAGGAGGGGGAGTGAGCCTCACCATGTG AGCTCTTCCTATCCCTCTCCTAACTGCCAGCCCCCTAAAAGCACTCCTGCTTAATCTTCAAAGCCTTCTCCCTAGCTCCTCCCCTTCCTCTTGTCTGATT TCTTAGGGGAAGGAGAAGTAAGAGGCTACCTCTTACCTAACATCTGACCTGGCATCTAATTCTGATTCTGGCTTTAAGCCTTCAAAACTATAGCTTGCAG AACTGTAGCTGCCATGGCTAGGTAGAAGTGAGCAAAAAAGAGTTGGGTGTCTCCTTAAGCTGCAGAGATTTCTCATTGACTTTTATAAAGCATGTTCACC CTTATAGTCTAAGACTATATATATAAATGTATAAATATACAGTATAGATTTTTGGGTGGGGGGCATTGAGTATTGTTTAAAATGTAATTTAAATGAAAGA AAATTGAGTTGCACTTATTGACCATTTTTTAATTTACTTGTTTTGGATGGCTTGTCTATACTCCTTCCCTTAAGGGGTATCATGTATGGTGATAGGTATC TAGAGCTTAATGCTACATGTGAGTGACGATGATGTACAGATTCTTTCAGTTCTTTGGATTCTAAATACATGCCACATCAAACCTTTGAGTAGATCCATTT CCATTGCTTATTATGTAGGTAAGACTGTAGATATGTATTCTTTTCTCAGTGTTGGTATATTTTATATTACTGACATTTCTTCTAGTGATGATGGTTCACG TTGGGGTGATTTAATCCAGTTATAAGAAGAAGTTCATGTCCAAACGTCCTCTTTAGTTTTTGGTTGGGAATGAGGAAAATTCTTAAAAGGCCCATAGCAG CCAGTTCAAAAACACCCGACGTCATGTATTTGAGCATATCAGTAACCCCCTTAAATTTAATACCAGATACCTTATCTTACAATATTGATTGGGAAAACAT TTGCTGCCATTACAGAGGTATTAAAACTAAATTTCACTACTAGATTGACTAACTCAAATACACATTTGCTACTGTTGTAAGAATTCTGATTGATTTGATT GGGATGAATGCCATCTATCTAGTTCTAACAGTGAAGTTTTACTGTCTATTAATATTCAGGGTAAATAGGAATCATTCAGAAATGTTGAGTCTGTACTAAA CAGTAAGATATCTCAATGAACCATAAATTCAACTTTGTAAAAATCTTTTGAAGCATAGATAATATTGTTTGGTAAATGTTTCTTTTGTTTGGTAAATGTT TCTTTTAAAGACCCTCCTATTCTATAAAACTCTGCATGTAGAGGCTTGTTTACCTTTCTCTCTCTAAGGTTTACAATAGGAGTGGTGATTTGAAAAATAT AAAATTATGAGATTGGTTTTCCTGTGGCATAAATTGCATCACTGTATCATTTTCTTTTTTAACCGGTAAGAGTTTCAGTTTGTTGGAAAGTAACTGTGAG AACCCAGTTTCCCGTCCATCTCCCTTAGGGACTACCCATAGACATGAAAGGTCCCCACAGAGCAAGAGATAAGTCTTTCATGGCTGCTGTTGCTTAAACC ACTTAAACGAAGAGTTCCCTTGAAACTTTGGGAAAACATGTTAATGACAATATTCCAGATCTTTCAGAAATATAACACATTTTTTTGCATGCATGCAAAT GAGCTCTGAAATCTTCCCATGCATTCTGGTCAAGGGCTGTCATTGCACATAAGCTTCCATTTTAATTTTAAAGTGCAAAAGGGCCAGCGTGGCTCTAAAA GGTAATGTGTGGATTGCCTCTGAAAAGTGTGTATATATTTTGTGTGAAATTGCATACTTTGTATTTTGATTATTTTTTTTTTCTTCTTGGGATAGTGGGA TTTCCAGAACCACACTTGAAACCTTTTTTTATCGTTTTTGTATTTTCATGAAAATACCATTTAGTAAGAATACCACATCAAATAAGAAATAATGCTACAA TTTTAAGAGGGGAGGGAAGGGAAAGTTTTTTTTTATTATTTTTTTAAAATTTTGTATGTTAAAGAGAATGAGTCCTTGATTTCAAAGTTTTGTTGTACTT AAATGGTAATAAGCACTGTAAACTTCTGCAACAAGCATGCAGCTTTGCAAACCCATTAAGGGGAAGAATGAAAGCTGTTCCTTGGTCCTAGTAAGAAGAC AAACTGCTTCCCTTACTTTGCTGAGGGTTTGAATAAACCTAGGACTTCCGAGCTATGTCAGTACTATTCAGGTAACACTAGGGCCTTGGAAATTCCTGTA CTGTGTCTCATGGATTTGGCACTAGCCAAAGCGAGGCACCCTTACTGGCTTACCTCCTCATGGCAGCCTACTCTCCTTGAGTGTATGAGTAGCCAGGGTA AGGGGTAAAAGGATAGTAAGCATAGAAACCACTAGAAAGTGGGCTTAATGGAGTTCTTGTGGCCTCAGCTCAATGCAGTTAGCTGAAGAATTGAAAAGTT TTTGTTTGGAGACGTTTATAAACAGAAATGGAAAGCAGAGTTTTCATTAAATCCTTTTACCTTTTTTTTTTCTTGGTAATCCCCTAAAATAACAGTATGT GGGATATTGAATGTTAAAGGGATATTTTTTTCTATTATTTTTATAATTGTACAAAATTAAGCAAATGTTAAAAGTTTTATATGCTTTATTAATGTTTTCA AAAGGTATTATACATGTGATACATTTTTTAAGCTTCAGTTGCTTGTCTTCTGGTACTTTCTGTTATGGGCTTTTGGGGAGCCAGAAGCCAATCTACAATC TCTTTTTGTTTGCCAGGACATGCAATAAAATTTAAAAAATAAATAAAAACTAATTAAGAAATTGTGTGTGTGTCTATCTGTGTCTGTTTGCCTATTTGTC CAT

TP73L.F10 (forward primer)
TP73L.R8 (reverse primer)
Single exon skipping

Frame 0

FRRNPSSFLLERKLLPIHHVPEHTDK*IPQSRG FPAYLGFSGTAYMFSSAH*LELCG*TIRRWCDKQ D*D*HGLYPHAGLGPE*PHVATVHEPGAPEQHG PADSERLLVHQSL*HRPRAEQRHGALALRTAQL HLRCSLSITRHPLQHRLPRPAQFRRVLPAVEHRQ VGHLDVFH*TEETLLPNCKDMPHPDQGDDPTSS GSCYPRHACLQKS*ARHGGGEAVPQP*AEP*IQ RGTDCPS*SFDSSRGEQPCPVCRRSHHRKTECAG TL*ATPGWH*IHDSLVQFHV*QQLCWRDEPPSN FNHCYSGNQRWASPGPTLL*GPDLCLPRKRQEG G*R*HQKAASFGQYKER*WYEAPVSSEHTWYPDD IHQETKIPR**TVILTSEGP*DL*NAVEDQRVP GTHAVPSSAHN*NVQATATAAAPALTSETDLNT VSIFIW*QLPTSEQNEQHEQAAFCEPAYQPSAAQ RPHSYNHS*WHGSQHSHDGHPHANGWRHEWTQP HPGTPSPTLHAIHLPLHTPTSVSHRLQHCQDLA SLKIPEQFRHAIWKGILDHRQLHEFSSPSHLLRT PSSASTVSVGSSETRGERVIDAVRFTLRQTISF PPRDEWNDFNFDMDARRNKQQRIKEEGE*ASPC ELFLSLS*LPAP*KHSCLIFKAFSLAPPLPLV*F LRGRRSKRLPLT*HLTWHLILILALSLQNYSLQ NCSCHG*VEVSKKELGVSLSCRDFSLTFIKHVH PYSLRLYI*MYKYTV*IFGWGALSIV*NVI*MKE N*VALIDHFLIYLFWMACLYSFP*GVSCMVIGI *SLMLHVSDDDVQILSVLWILNTCHIKPLSRSI SIAYYVGKTVDMYSFLSVGIFYITDISSSDDGSR WGDLIQL*EEVHVQTSSLVFGWE*GKFLKGP*Q PVQKHPTSCI*AYQ*PP*I*YQIPYLTILIGKT FAAITEVLKLNFTTRLTNSNTHLLLL*EF*LI*L G*MPSI*F*Q*SFTVY*YSG*IGIIQKC*VCTK Q*DISMNHKFNFVKIF*SIDNIVW*MFLLFGKC FF*RPSYSIKLCM*RLVYLSLSKVYNRSGDLKNI KL*DWFSCGINCITVSFSFLTGKSFSLLESNCE NPVSRPSPLGTTHRHERSPQSKR*VFHGCCCLN HLNEEFP*NFGKTC**QYSRSFRNITHFFACMQM SSEIFPCILVKGCHCT*ASILILKCKRASVALK GNVWIASEKCVYILCEIAYFVF*LFFFSSWDSG ISRTTLETFFYRFCIFMKIPFSKNTTSNKK*CYN FKRGGKGKFFFIIFLKFCMLKRMSP*FQSFVVL KW**AL*TSATSMQLCKPIKGKNESCSLVLVRR QTASLTLLRV*INLGLPSYVSTIQVTLGPWKFLY CVSWIWH*PKRGTLTGLPPHGSLLSLSV*VARV RGKRIVSIETTRKWA*WSSCGLSSMQLAEELKS FCLETFINRNGKQSFH*ILLPFFFLGNPLK*QYV GY*MLKGYFFLLFL*LYKIKQMLKVLYALLMFS KGIIHVIHFLSFSCLSSGTFCYGLLGSQKPIYN LFLFARTCNKI*KINKN*LRNCVCVYLCLFAYLS

Frame 1

FVETPAHFSWKESYYRSTMSQSTQTNEFLSPEV FQHIWDFLEQPICSVQPIDLNFVDEPSEDGATN KIEISMDCIRMQDSDLSDPMWPQYTNLGLLNSMD QQIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSS TFDALSPSPAIPSNTDYPGPHSFDVSFQQSSTA KSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQ GAVIRAMPVYKKAEHVTEVVKRCPNHELSREFN EGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVL VPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPI LIIVTLETRDGQVLGRRCFEARICACPGRDRKA DEDSIRKQQVSDSTKNGDGTKRPFRQNTHGIQM TSIKKRRSPDDELLYLPVRGRETYEMLLKIKESL ELMQYLPQHTIETYRQQQQQQHQHLLQKQTSIQ SPSSYGNSSPPLNKMNSMNKLPSVSQLINPQQR NALTPTTIPDGMGANIPMMGTHMPMAGDMNGLSP TQALPPPLSMPSTSHCTPPPPYPTDCSIVRIWQ V*KSLSNFDMRSGRASWTTGSSTNSPPLLISCG PQAVPLQSVWAPVRPGVSVLLMLCDSPSARPSLS HPEMSGMTSTLTWMLAAISNSASKRRGSEPHHV SSSYPSPNCQPPKSTPA*SSKPSP*LLPFLLSD FLGEGEVRGYLLPNI*PGI*F*FWL*AFKTIACR TVAAMAR*K*AKKSWVSP*AAEISH*LL*SMFT LIV*DYIYKCINIQYRFLGGGH*VLFKM*FK*K KIELHLLTIF*FTCFGWLVYTPSLKGYHVW**VS RA*CYM*VTMMYRFFQFFGF*IHATSNL*VDPF PLLIM*VRL*ICILFSVLVYFILLTFLLVMMVH VGVI*SSYKKKFMSKRPL*FLVGNEENS*KAHSS QFKNTRRHVFEHISNPLKFNTRYLILQY*LGKH LLPLQRY*N*ISLLD*LTQIHICYCCKNSD*FD WDECHLSSSNSEVLLSINIQGK*ESFRNVESVLN SKISQ*TINSTL*KSFEA*IILFGKCFFCLVNV SFKDPPIL*NSACRGLFTFLSLRFTIGVVI*KI *NYEIGFPVA*IASLYHFLF*PVRVSVCWKVTVR TQFPVHLP*GLPIDMKGPHRARDKSFMAAVA*T T*TKSSLETLGKHVNDNIPDLSEI*HIFLHACK *ALKSSHAFWSRAVIAHKLPF*F*SAKGPAWL*K VMCGLPLKSVYIFCVKLHTLYFDYFFFLLGIVG FPEPHLKPFFIVFVFS*KYHLVRIPHQIRNNAT ILRGEGRESFFLLFF*NFVC*RE*VLDFKVLLYL NGNKHCKLLQQACSFANPLRGRMKAVPWS**ED KLLPLLC*GFE*T*DFRAMSVLFR*H*GLGNSC TVSHGFGTSQSEAPLLAYLLMAAYSP*VYE*PG* GVKG**A*KPLESGLNGVLVASAQCS*LKN*KV FVWRRL*TEMESRVFIKSFYLFFFLVIP*NNSM WDIEC*RDIFFYYFYNCTKLSKC*KFYMLY*CFQ KVLYM*YIF*ASVACLLVLSVMGFWGARSQSTI SFCLPGHAIKFKK*IKTN*EIVCVSICVCLPIC P

Frame 2

S*KPQLISLGKKVITDPPCPRAHRQMNSSVQRF SSISGIFWNSLYVQFSPLT*TLWMNHQKMVRQT RLRLAWTVSACRTRT*VTPCGHSTRTWGS*TAW TSRFRTAPRPPVPITQTTRRTASRRPRPTHSPAP PSMLSLHHPPSPPTPTTQARTVSTCPSSSRAPP SRPPGRIPLN*RNSTAKLQRHAPSRSR**PHLL RELLSAPCLSTKKLSTSRRW*SGAPTMS*AVNST RDRLPLLVI*FE*RGTAMPSM*KIPSQEDRVCW YLMSHPRLALNSRQSCTISCVTAVVLEG*TAVQ F*SLLLWKPEMGKSWADAALRPGSVLAQEETGRR MKIASESSKFRTVQRTVMVRSARFVRTHMVSR* HPSRNEDPQMMNCYTYQ*GAVRLMKCC*RSKSP WNSCSTFLSTQLKRTGNSNSSSTSTYFRNRPQYS LHLHMVTAPHL*TK*TA*TSCLL*ASLSTLSSA TPSLLQPFLMAWEPTFP*WAPTCQWLET*MDSA PPRHSLPHSPCHPPPTAHPHLRIPQIAALSGSGK SENP*AISTCDLEGHPGPPAAPRILLPFSSPAD PKQCLYSQCGLQ*DPG*ACY*CCAIHPPPDHLF PTPR*VE*LQL*HGCSPQ*ATAHQRGGGVSLTM* ALPIPLLTASPLKALLLNLQSLLPSSSPSSCLI S*GKEK*EATSYLTSDLASNSDSGFKPSKL*LA EL*LPWLGRSEQKRVGCLLKLQRFLIDFYKACSP L*SKTIYINV*IYSIDFWVGGIEYCLKCNLNER KLSCTY*PFFNLLVLDGLSILLPLRGIMYGDRY LELNATCE*R*CTDSFSSLDSKYMPHQTFE*IHF HCLLCR*DCRYVFFSQCWYILYY*HFF***WFT LG*FNPVIRRSSCPNVLFSFWLGMRKILKRPIA ASSKTPDVMYLSISVTPLNLIPDTLSYNIDWENI CCHYRGIKTKFHY*ID*LKYTFATVVRILIDLI GMNAIYLVLTVKFYCLLIFRVNRNHSEMLSLY* TVRYLNEP*IQLCKNLLKHR*YCLVNVSFVW*MF LLKTLLFYKTLHVEACLPFSL*GLQ*EW*FEKY KIMRLVFLWHKLHHCIIFFFNR*EFQFVGK*L* EPSFPSISLRDYP*T*KVPTEQEISLSWLLLLKP LKRRVPLKLWENMLMTIFQIFQKYNTFFCMHAN EL*NLPMHSGQGLSLHISFHFNFKVQKGQRGSK R*CVDCL*KVCIYFV*NCILCILIIFFFFLG*WD FQNHT*NLFLSFLYFHENTI**EYHIK*EIMLQ F*EGREGKVFFYYFFKILYVKENESLISKFCCT *MVISTVNFCNKHAALQTH*GEE*KLFLGPSKKT NCFPYFAEGLNKPRTSELCQYYSGNTRALEIPV LCLMDLALAKARHPYWLTSSWQPTLLECMSSQG KG*KDSKHRNH*KVGLMEFLWPQLNAVS*RIEKF LFGDVYKQKWKAEFSLNPFTFFFSW*SPKITVC GILNVKGIFFSIIFIIVQN*ANVKSFICFINVF KRYYTCDTFFKLQLLVFWYFLLWAFGEPEANLQS LFVCQDMQ*NLKNK*KLIKKLCVCLSVSVCLFV H

Sequence TP73L.dAug05

AGAGAGAAAGAGAGAGAGGGACTTGAGTTCTGTTATCTTCTTAAGTAGATTCATATTGTAAGGGTCTCGGGGTGGGGGGGTTGGCAAAATCCTGGAGCCA GAAGAAAGGACAGCAGCATTGATCAATCTTACAGCTAACATGTTGTACCTGGAAAACAATGCCCAGACTCAATTTAGTGAGTATTCCACTGAACTGAAGA AACTCTACTGCCAAATTGCAAAGACATGCCCCATCCAGATCAAGGTGATGACCCCACCTCCTCAGGGAGCTGTTATCCGCGCCATGCCTGTCTACAAAAA AGCTGAGCACGTCACGGAGGTGGTGAAGCGGTGCCCCAACCATGAGCTGAGCCGTGAATTCAACGAGGGACAGATTGCCCCTCCTAGTCATTTGATTCGA GTAGAGGGGAACAGCCATGCCCAGTATGTAGAAGATCCCATCACAGGAAGACAGAGTGTGCTGGTACCTTATGAGCCACCCCAGGTTGGCACTGAATTCA CGACAGTCTTGTACAATTTCATGTGTAACAGCAGTTGTGTTGGAGGGATGAACCGCCGTCCAATTTTAATCATTGTTACTCTGGAAACCAGAGATGGGCA AGTCCTGGGCCGACGCTGCTTTGAGGCCCGGATCTGTGCTTGCCCAGGAAGAGACAGGAAGGCGGATGAAGATAGCATCAGAAAGCAGCAAGTTTCGGAC AGTACAAAGAACGGTGATGGTACGAAGCGCCCGTTTCGTCAGAACACACATGGTATCCAGATGACATCCATCAAGAAACGAAGATCCCCAGATGATGAAC TGTTATACTTACCAGTGAGGGGCCGTGAGACTTATGAAATGCTGTTGAAGATCAAAGAGTCCCTGGAACTCATGCAGTACCTTCCTCAGCACACAATTGA AACGTACAGGCAACAGCAACAGCAGCAGCACCAGCACTTACTTCAGAAACAGACCTCAATACAGTCTCCATCTTCATATGGTAACAGCTCCCCACCTCTG AACAAAATGAACAGCATGAACAAGCTGCCTTCTGTGAGCCAGCTTATCAACCCTCAGCAGCGCAACGCCCTCACTCCTACAACCATTCCTGATGGCATGG GAGCCAACATTCCCATGATGGGCACCCACATGCCAATGGCTGGAGACATGAATGGACTCAGCCCCACCCAGGCACTCCCTCCCCCACTCTCCATGCCATC CACCTCCCACTGCACACCCCCACCTCCGTATCCCACAGATTGCAGCATTGTCAGTTTCTTAGCGAGGTTGGGCTGTTCATCATGTCTGGACTATTTCACG ACCCAGGGGCTGACCACCATCTATCAGATTGAGCATTACTCCATGGATGATCTGGCAAGTCTGAAAATCCCTGAGCAATTTCGACATGCGATCTGGAAGG GCATCCTGGACCACCGGCAGCTCCACGAATTCTCCTCCCCTTCTCATCTCCTGCGGACCCCAAGCAGTGCCTCTACAGTCAGTGTGGGCTCCAGTGAGAC CCGGGGTGAGCGTGTTATTGATGCTGTGCGATTCACCCTCCGCCAGACCATCTCTTTCCCACCCCGAGATGAGTGGAATGACTTCAACTTTGACATGGAT GCTCGCCGCAATAAGCAACAGCGCATCAAAGAGGAGGGGGAGTGAGCCTCACCATGTGAGCTCTTCCTATCCCTCTCCTAACTGCCAGCCCCCTAAAAGC ACTCCTGCTTAATCTTCAAAGCCTTCTCCCTAGCTCCTCCCCTTCCTCTTGTCTGATTTCTTAGGGGAAGGAGAAGTAAGAGGCTACCTCTTACCTAACA TCTGACCTGGCATCTAATTCTGATTCTGGCTTTAAGCCTTCAAAACTATAGCTTGCAGAACTGTAGCTGCCATGGCTAGGTAGAAGTGAGCAAAAAAGAG TTGGGTGTCTCCTTAAGCTGCAGAGATTTCTCATTGACTTTTATAAAGCATGTTCACCCTTATAGTCTAAGACTATATATATAAATGTATAAATATACAG TATAGATTTTTGGGTGGGGGGCATTGAGTATTGTTTAAAATGTAATTTAAATGAAAGAAAATTGAGTTGCACTTATTGACCATTTTTTAATTTACTTGTT TTGGATGGCTTGTCTATACTCCTTCCCTTAAGGGGTATCATGTATGGTGATAGGTATCTAGAGCTTAATGCTACATGTGAGTGACGATGATGTACAGATT CTTTCAGTTCTTTGGATTCTAAATACATGCCACATCAAACCTTTGAGTAGATCCATTTCCATTGCTTATTATGTAGGTAAGACTGTAGATATGTATTCTT TTCTCAGTGTTGGTATATTTTATATTACTGACATTTCTTCTAGTGATGATGGTTCACGTTGGGGTGATTTAATCCAGTTATAAGAAGAAGTTCATGTCCA AACGTCCTCTTTAGTTTTTGGTTGGGAATGAGGAAAATTCTTAAAAGGCCCATAGCAGCCAGTTCAAAAACACCCGACGTCATGTATTTGAGCATATCAG TAACCCCCTTAAATTTAATACCAGATACCTTATCTTACAATATTGATTGGGAAAACATTTGCTGCCATTACAGAGGTATTAAAACTAAATTTCACTACTA GATTGACTAACTCAAATACACATTTGCTACTGTTGTAAGAATTCTGATTGATTTGATTGGGATGAATGCCATCTATCTAGTTCTAACAGTGAAGTTTTAC TGTCTATTAATATTCAGGGTAAATAGGAATCATTCAGAAATGTTGAGTCTGTACTAAACAGTAAGATATCTCAATGAACCATAAATTCAACTTTGTAAAA ATCTTTTGAAGCATAGATAATATTGTTTGGTAAATGTTTCTTTTGTTTGGTAAATGTTTCTTTTAAAGACCCTCCTATTCTATAAAACTCTGCATGTAGA GGCTTGTTTACCTTTCTCTCTCTAAGGTTTACAATAGGAGTGGTGATTTGAAAAATATAAAATTATGAGATTGGTTTTCCTGTGGCATAAATTGCATCAC TGTATCATTTTCTTTTTTAACCGGTAAGAGTTTCAGTTTGTTGGAAAGTAACTGTGAGAACCCAGTTTCCCGTCCATCTCCCTTAGGGACTACCCATAGA CATGAAAGGTCCCCACAGAGCAAGAGATAAGTCTTTCATGGCTGCTGTTGCTTAAACCACTTAAACGAAGAGTTCCCTTGAAACTTTGGGAAAACATGTT AATGACAATATTCCAGATCTTTCAGAAATATAACACATTTTTTTGCATGCATGCAAATGAGCTCTGAAATCTTCCCATGCATTCTGGTCAAGGGCTGTCA TTGCACATAAGCTTCCATTTTAATTTTAAAGTGCAAAAGGGCCAGCGTGGCTCTAAAAGGTAATGTGTGGATTGCCTCTGAAAAGTGTGTATATATTTTG TGTGAAATTGCATACTTTGTATTTTGATTATTTTTTTTTTCTTCTTGGGATAGTGGGATTTCCAGAACCACACTTGAAACCTTTTTTTATCGTTTTTGTA TTTTCATGAAAATACCATTTAGTAAGAATACCACATCAAATAAGAAATAATGCTACAATTTTAAGAGGGGAGGGAAGGGAAAGTTTTTTTTTATTATTTT TTTAAAATTTTGTATGTTAAAGAGAATGAGTCCTTGATTTCAAAGTTTTGTTGTACTTAAATGGTAATAAGCACTGTAAACTTCTGCAACAAGCATGCAG CTTTGCAAACCCATTAAGGGGAAGAATGAAAGCTGTTCCTTGGTCCTAGTAAGAAGACAAACTGCTTCCCTTACTTTGCTGAGGGTTTGAATAAACCTAG GACTTCCGAGCTATGTCAGTACTATTCAGGTAACACTAGGGCCTTGGAAATTCCTGTACTGTGTCTCATGGATTTGGCACTAGCCAAAGCGAGGCACCCT TACTGGCTTACCTCCTCATGGCAGCCTACTCTCCTTGAGTGTATGAGTAGCCAGGGTAAGGGGTAAAAGGATAGTAAGCATAGAAACCACTAGAAAGTGG GCTTAATGGAGTTCTTGTGGCCTCAGCTCAATGCAGTTAGCTGAAGAATTGAAAAGTTTTTGTTTGGAGACGTTTATAAACAGAAATGGAAAGCAGAGTT TTCATTAAATCCTTTTACCTTTTTTTTTTCTTGGTAATCCCCTAAAATAACAGTATGTGGGATATTGAATGTTAAAGGGATATTTTTTTCTATTATTTTT ATAATTGTACAAAATTAAGCAAATGTTAAAAGTTTTATATGCTTTATTAATGTTTTCAAAAGGTATTATACATGTGATACATTTTTTAAGCTTCAGTTGC TTGTCTTCTGGTACTTTCTGTTATGGGCTTTTGGGGAGCCAGAAGCCAATCTACAATCTCTTTTTGTTTGCCAGGACATGCAATAAAATTTAAAAAATAA ATAAAAACTAATTAAGAAATTGTGTGTGTGTCTATCTGTGTCTGTTTGCCTATTTGTCCAT

TP73L.F10 (forward primer)
TP73L.R8 (reverse primer)
Single exon skipping

Frame 0

REKEREGLEFCYLLK*IHIVRVSGWGGWQNPGA RRKDSSIDQSYS*HVVPGKQCPDSI**VFH*TEE TLLPNCKDMPHPDQGDDPTSSGSCYPRHACLQK S*ARHGGGEAVPQP*AEP*IQRGTDCPS*SFDS SRGEQPCPVCRRSHHRKTECAGTL*ATPGWH*IH DSLVQFHV*QQLCWRDEPPSNFNHCYSGNQRWA SPGPTLL*GPDLCLPRKRQEGG*R*HQKAASFG QYKER*WYEAPVSSEHTWYPDDIHQETKIPR**T VILTSEGP*DL*NAVEDQRVPGTHAVPSSAHN* NVQATATAAAPALTSETDLNTVSIFIW*QLPTS EQNEQHEQAAFCEPAYQPSAAQRPHSYNHS*WHG SQHSHDGHPHANGWRHEWTQPHPGTPSPTLHAI HLPLHTPTSVSHRLQHCQFLSEVGLFIMSGLFH DPGADHHLSD*ALLHG*SGKSENP*AISTCDLEG HPGPPAAPRILLPFSSPADPKQCLYSQCGLQ*D PG*ACY*CCAIHPPPDHLFPTPR*VE*LQL*HG CSPQ*ATAHQRGGGVSLTM*ALPIPLLTASPLKA LLLNLQSLLPSSSPSSCLIS*GKEK*EATSYLT SDLASNSDSGFKPSKL*LAEL*LPWLGRSEQKR VGCLLKLQRFLIDFYKACSPL*SKTIYINV*IYS IDFWVGGIEYCLKCNLNERKLSCTY*PFFNLLV LDGLSILLPLRGIMYGDRYLELNATCE*R*CTD SFSSLDSKYMPHQTFE*IHFHCLLCR*DCRYVFF SQCWYILYY*HFF***WFTLG*FNPVIRRSSCP NVLFSFWLGMRKILKRPIAASSKTPDVMYLSIS VTPLNLIPDTLSYNIDWENICCHYRGIKTKFHY* ID*LKYTFATVVRILIDLIGMNAIYLVLTVKFY CLLIFRVNRNHSEMLSLY*TVRYLNEP*IQLCK NLLKHR*YCLVNVSFVW*MFLLKTLLFYKTLHVE ACLPFSL*GLQ*EW*FEKYKIMRLVFLWHKLHH CIIFFFNR*EFQFVGK*L*EPSFPSISLRDYP* T*KVPTEQEISLSWLLLLKPLKRRVPLKLWENML MTIFQIFQKYNTFFCMHANEL*NLPMHSGQGLS LHISFHFNFKVQKGQRGSKR*CVDCL*KVCIYF V*NCILCILIIFFFFLG*WDFQNHT*NLFLSFLY FHENTI**EYHIK*EIMLQF*EGREGKVFFYYF FKILYVKENESLISKFCCT*MVISTVNFCNKHA ALQTH*GEE*KLFLGPSKKTNCFPYFAEGLNKPR TSELCQYYSGNTRALEIPVLCLMDLALAKARHP YWLTSSWQPTLLECMSSQGKG*KDSKHRNH*KV GLMEFLWPQLNAVS*RIEKFLFGDVYKQKWKAEF SLNPFTFFFSW*SPKITVCGILNVKGIFFSIIF IIVQN*ANVKSFICFINVFKRYYTCDTFFKLQL LVFWYFLLWAFGEPEANLQSLFVCQDMQ*NLKNK *KLIKKLCVCLSVSVCLFVH

Frame 1

ERKRERDLSSVIFLSRFIL*GSRGGGVGKILEP EERTAALINLTANMLYLENNAQTQFSEYSTELK KLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKK AEHVTEVVKRCPNHELSREFNEGQIAPPSHLIR VEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEF TTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQ VLGRRCFEARICACPGRDRKADEDSIRKQQVSD STKNGDGTKRPFRQNTHGIQMTSIKKRRSPDDE LLYLPVRGRETYEMLLKIKESLELMQYLPQHTIE TYRQQQQQQHQHLLQKQTSIQSPSSYGNSSPPL NKMNSMNKLPSVSQLINPQQRNALTPTTIPDGM GANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPS TSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFT TQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWK GILDHRQLHEFSSPSHLLRTPSSASTVSVGSSET RGERVIDAVRFTLRQTISFPPRDEWNDFNFDMD ARRNKQQRIKEEGE*ASPCELFLSLS*LPAP*K HSCLIFKAFSLAPPLPLV*FLRGRRSKRLPLT*H LTWHLILILALSLQNYSLQNCSCHG*VEVSKKE LGVSLSCRDFSLTFIKHVHPYSLRLYI*MYKYT V*IFGWGALSIV*NVI*MKEN*VALIDHFLIYLF WMACLYSFP*GVSCMVIGI*SLMLHVSDDDVQI LSVLWILNTCHIKPLSRSISIAYYVGKTVDMYS FLSVGIFYITDISSSDDGSRWGDLIQL*EEVHVQ TSSLVFGWE*GKFLKGP*QPVQKHPTSCI*AYQ *PP*I*YQIPYLTILIGKTFAAITEVLKLNFTT RLTNSNTHLLLL*EF*LI*LG*MPSI*F*Q*SFT VY*YSG*IGIIQKC*VCTKQ*DISMNHKFNFVK IF*SIDNIVW*MFLLFGKCFF*RPSYSIKLCM* RLVYLSLSKVYNRSGDLKNIKL*DWFSCGINCIT VSFSFLTGKSFSLLESNCENPVSRPSPLGTTHR HERSPQSKR*VFHGCCCLNHLNEEFP*NFGKTC **QYSRSFRNITHFFACMQMSSEIFPCILVKGCH CT*ASILILKCKRASVALKGNVWIASEKCVYIL CEIAYFVF*LFFFSSWDSGISRTTLETFFYRFC IFMKIPFSKNTTSNKK*CYNFKRGGKGKFFFIIF LKFCMLKRMSP*FQSFVVLKW**AL*TSATSMQ LCKPIKGKNESCSLVLVRRQTASLTLLRV*INL GLPSYVSTIQVTLGPWKFLYCVSWIWH*PKRGTL TGLPPHGSLLSLSV*VARVRGKRIVSIETTRKW A*WSSCGLSSMQLAEELKSFCLETFINRNGKQS FH*ILLPFFFLGNPLK*QYVGY*MLKGYFFLLFL *LYKIKQMLKVLYALLMFSKGIIHVIHFLSFSC LSSGTFCYGLLGSQKPIYNLFLFARTCNKI*KI NKN*LRNCVCVYLCLFAYLS

Frame 2

RERERGT*VLLSS*VDSYCKGLGVGGLAKSWSQ KKGQQH*SILQLTCCTWKTMPRLNLVSIPLN*R NSTAKLQRHAPSRSR**PHLLRELLSAPCLSTK KLSTSRRW*SGAPTMS*AVNSTRDRLPLLVI*FE *RGTAMPSM*KIPSQEDRVCWYLMSHPRLALNS RQSCTISCVTAVVLEG*TAVQF*SLLLWKPEMG KSWADAALRPGSVLAQEETGRRMKIASESSKFRT VQRTVMVRSARFVRTHMVSR*HPSRNEDPQMMN CYTYQ*GAVRLMKCC*RSKSPWNSCSTFLSTQL KRTGNSNSSSTSTYFRNRPQYSLHLHMVTAPHL* TK*TA*TSCLL*ASLSTLSSATPSLLQPFLMAW EPTFP*WAPTCQWLET*MDSAPPRHSLPHSPCH PPPTAHPHLRIPQIAALSVS*RGWAVHHVWTISR PRG*PPSIRLSITPWMIWQV*KSLSNFDMRSGR ASWTTGSSTNSPPLLISCGPQAVPLQSVWAPVR PGVSVLLMLCDSPSARPSLSHPEMSGMTSTLTWM LAAISNSASKRRGSEPHHVSSSYPSPNCQPPKS TPA*SSKPSP*LLPFLLSDFLGEGEVRGYLLPN I*PGI*F*FWL*AFKTIACRTVAAMAR*K*AKKS WVSP*AAEISH*LL*SMFTLIV*DYIYKCINIQ YRFLGGGH*VLFKM*FK*KKIELHLLTIF*FTC FGWLVYTPSLKGYHVW**VSRA*CYM*VTMMYRF FQFFGF*IHATSNL*VDPFPLLIM*VRL*ICIL FSVLVYFILLTFLLVMMVHVGVI*SSYKKKFMS KRPL*FLVGNEENS*KAHSSQFKNTRRHVFEHIS NPLKFNTRYLILQY*LGKHLLPLQRY*N*ISLL D*LTQIHICYCCKNSD*FDWDECHLSSSNSEVL LSINIQGK*ESFRNVESVLNSKISQ*TINSTL*K SFEA*IILFGKCFFCLVNVSFKDPPIL*NSACR GLFTFLSLRFTIGVVI*KI*NYEIGFPVA*IAS LYHFLF*PVRVSVCWKVTVRTQFPVHLP*GLPID MKGPHRARDKSFMAAVA*TT*TKSSLETLGKHV NDNIPDLSEI*HIFLHACK*ALKSSHAFWSRAV IAHKLPF*F*SAKGPAWL*KVMCGLPLKSVYIFC VKLHTLYFDYFFFLLGIVGFPEPHLKPFFIVFV FS*KYHLVRIPHQIRNNATILRGEGRESFFLLF F*NFVC*RE*VLDFKVLLYLNGNKHCKLLQQACS FANPLRGRMKAVPWS**EDKLLPLLC*GFE*T* DFRAMSVLFR*H*GLGNSCTVSHGFGTSQSEAP LLAYLLMAAYSP*VYE*PG*GVKG**A*KPLESG LNGVLVASAQCS*LKN*KVFVWRRL*TEMESRV FIKSFYLFFFLVIP*NNSMWDIEC*RDIFFYYF YNCTKLSKC*KFYMLY*CFQKVLYM*YIF*ASVA CLLVLSVMGFWGARSQSTISFCLPGHAIKFKK* IKTN*EIVCVSICVCLPICP

Sequence TP73L.bAug05

AGAGAGAAAGAGAGAGAGGGACTTGAGTTCTGTTATCTTCTTAAGTAGATTCATATTGTAAGGGTCTCGGGGTGGGGGGGTTGGCAAAATCCTGGAGCCA GAAGAAAGGACAGCAGCATTGATCAATCTTACAGCTAACATGTTGTACCTGGAAAACAATGCCCAGACTCAATTTAGTGAGCCACAGTACACGAACCTGG GGCTCCTGAACAGCATGGACCAGCAGATTCAGAACGGCTCCTCGTCCACCAGTCCCTATAACACAGACCACGCGCAGAACAGCGTCACGGCGCCCTCGCC CTACGCACAGCCCAGCTCCACCTTCGATGCTCTCTCTCCATCACCCGCCATCCCCTCCAACACCGACTACCCAGGCCCGCACAGTTTCGACGTGTCCTTC CAGCAGTCGAGCACCGCCAAGTCGGCCACCTGGACGTATTCCACTGAACTGAAGAAACTCTACTGCCAAATTGCAAAGACATGCCCCATCCAGATCAAGG TGATGACCCCACCTCCTCAGGGAGCTGTTATCCGCGCCATGCCTGTCTACAAAAAAGCTGAGCACGTCACGGAGGTGGTGAAGCGGTGCCCCAACCATGA GCTGAGCCGTGAATTCAACGAGGGACAGATTGCCCCTCCTAGTCATTTGATTCGAGTAGAGGGGAACAGCCATGCCCAGTATGTAGAAGATCCCATCACA GGAAGACAGAGTGTGCTGGTACCTTATGAGCCACCCCAGGTTGGCACTGAATTCACGACAGTCTTGTACAATTTCATGTGTAACAGCAGTTGTGTTGGAG GGATGAACCGCCGTCCAATTTTAATCATTGTTACTCTGGAAACCAGAGATGGGCAAGTCCTGGGCCGACGCTGCTTTGAGGCCCGGATCTGTGCTTGCCC AGGAAGAGACAGGAAGGCGGATGAAGATAGCATCAGAAAGCAGCAAGTTTCGGACAGTACAAAGAACGGTGATGGTACGAAGCGCCCGTTTCGTCAGAAC ACACATGGTATCCAGATGACATCCATCAAGAAACGAAGATCCCCAGATGATGAACTGTTATACTTACCAGTGAGGGGCCGTGAGACTTATGAAATGCTGT TGAAGATCAAAGAGTCCCTGGAACTCATGCAGTACCTTCCTCAGCACACAATTGAAACGTACAGGCAACAGCAACAGCAGCAGCACCAGCACTTACTTCA GAAACAGACCTCAATACAGTCTCCATCTTCATATGGTAACAGCTCCCCACCTCTGAACAAAATGAACAGCATGAACAAGCTGCCTTCTGTGAGCCAGCTT ATCAACCCTCAGCAGCGCAACGCCCTCACTCCTACAACCATTCCTGATGGCATGGGAGCCAACATTCCCATGATGGGCACCCACATGCCAATGGCTGGAG ACATGAATGGACTCAGCCCCACCCAGGCACTCCCTCCCCCACTCTCCATGCCATCCACCTCCCACTGCACACCCCCACCTCCGTATCCCACAGATTGCAG CATTGTCAGTTTCTTAGCGAGGTTGGGCTGTTCATCATGTCTGGACTATTTCACGACCCAGGGGCTGACCACCATCTATCAGATTGAGCATTACTCCATG GATGATCTGGCAAGTCTGAAAATCCCTGAGCAATTTCGACATGCGATCTGGAAGGGCATCCTGGACCACCGGCAGCTCCACGAATTCTCCTCCCCTTCTC ATCTCCTGCGGACCCCAAGCAGTGCCTCTACAGTCAGTGTGGGCTCCAGTGAGACCCGGGGTGAGCGTGTTATTGATGCTGTGCGATTCACCCTCCGCCA GACCATCTCTTTCCCACCCCGAGATGAGTGGAATGACTTCAACTTTGACATGGATGCTCGCCGCAATAAGCAACAGCGCATCAAAGAGGAGGGGGAGTGA GCCTCACCATGTGAGCTCTTCCTATCCCTCTCCTAACTGCCAGCCCCCTAAAAGCACTCCTGCTTAATCTTCAAAGCCTTCTCCCTAGCTCCTCCCCTTC CTCTTGTCTGATTTCTTAGGGGAAGGAGAAGTAAGAGGCTACCTCTTACCTAACATCTGACCTGGCATCTAATTCTGATTCTGGCTTTAAGCCTTCAAAA CTATAGCTTGCAGAACTGTAGCTGCCATGGCTAGGTAGAAGTGAGCAAAAAAGAGTTGGGTGTCTCCTTAAGCTGCAGAGATTTCTCATTGACTTTTATA AAGCATGTTCACCCTTATAGTCTAAGACTATATATATAAATGTATAAATATACAGTATAGATTTTTGGGTGGGGGGCATTGAGTATTGTTTAAAATGTAA TTTAAATGAAAGAAAATTGAGTTGCACTTATTGACCATTTTTTAATTTACTTGTTTTGGATGGCTTGTCTATACTCCTTCCCTTAAGGGGTATCATGTAT GGTGATAGGTATCTAGAGCTTAATGCTACATGTGAGTGACGATGATGTACAGATTCTTTCAGTTCTTTGGATTCTAAATACATGCCACATCAAACCTTTG AGTAGATCCATTTCCATTGCTTATTATGTAGGTAAGACTGTAGATATGTATTCTTTTCTCAGTGTTGGTATATTTTATATTACTGACATTTCTTCTAGTG ATGATGGTTCACGTTGGGGTGATTTAATCCAGTTATAAGAAGAAGTTCATGTCCAAACGTCCTCTTTAGTTTTTGGTTGGGAATGAGGAAAATTCTTAAA AGGCCCATAGCAGCCAGTTCAAAAACACCCGACGTCATGTATTTGAGCATATCAGTAACCCCCTTAAATTTAATACCAGATACCTTATCTTACAATATTG ATTGGGAAAACATTTGCTGCCATTACAGAGGTATTAAAACTAAATTTCACTACTAGATTGACTAACTCAAATACACATTTGCTACTGTTGTAAGAATTCT GATTGATTTGATTGGGATGAATGCCATCTATCTAGTTCTAACAGTGAAGTTTTACTGTCTATTAATATTCAGGGTAAATAGGAATCATTCAGAAATGTTG AGTCTGTACTAAACAGTAAGATATCTCAATGAACCATAAATTCAACTTTGTAAAAATCTTTTGAAGCATAGATAATATTGTTTGGTAAATGTTTCTTTTG TTTGGTAAATGTTTCTTTTAAAGACCCTCCTATTCTATAAAACTCTGCATGTAGAGGCTTGTTTACCTTTCTCTCTCTAAGGTTTACAATAGGAGTGGTG ATTTGAAAAATATAAAATTATGAGATTGGTTTTCCTGTGGCATAAATTGCATCACTGTATCATTTTCTTTTTTAACCGGTAAGAGTTTCAGTTTGTTGGA AAGTAACTGTGAGAACCCAGTTTCCCGTCCATCTCCCTTAGGGACTACCCATAGACATGAAAGGTCCCCACAGAGCAAGAGATAAGTCTTTCATGGCTGC TGTTGCTTAAACCACTTAAACGAAGAGTTCCCTTGAAACTTTGGGAAAACATGTTAATGACAATATTCCAGATCTTTCAGAAATATAACACATTTTTTTG CATGCATGCAAATGAGCTCTGAAATCTTCCCATGCATTCTGGTCAAGGGCTGTCATTGCACATAAGCTTCCATTTTAATTTTAAAGTGCAAAAGGGCCAG CGTGGCTCTAAAAGGTAATGTGTGGATTGCCTCTGAAAAGTGTGTATATATTTTGTGTGAAATTGCATACTTTGTATTTTGATTATTTTTTTTTTCTTCT TGGGATAGTGGGATTTCCAGAACCACACTTGAAACCTTTTTTTATCGTTTTTGTATTTTCATGAAAATACCATTTAGTAAGAATACCACATCAAATAAGA AATAATGCTACAATTTTAAGAGGGGAGGGAAGGGAAAGTTTTTTTTTATTATTTTTTTAAAATTTTGTATGTTAAAGAGAATGAGTCCTTGATTTCAAAG TTTTGTTGTACTTAAATGGTAATAAGCACTGTAAACTTCTGCAACAAGCATGCAGCTTTGCAAACCCATTAAGGGGAAGAATGAAAGCTGTTCCTTGGTC CTAGTAAGAAGACAAACTGCTTCCCTTACTTTGCTGAGGGTTTGAATAAACCTAGGACTTCCGAGCTATGTCAGTACTATTCAGGTAACACTAGGGCCTT GGAAATTCCTGTACTGTGTCTCATGGATTTGGCACTAGCCAAAGCGAGGCACCCTTACTGGCTTACCTCCTCATGGCAGCCTACTCTCCTTGAGTGTATG AGTAGCCAGGGTAAGGGGTAAAAGGATAGTAAGCATAGAAACCACTAGAAAGTGGGCTTAATGGAGTTCTTGTGGCCTCAGCTCAATGCAGTTAGCTGAA GAATTGAAAAGTTTTTGTTTGGAGACGTTTATAAACAGAAATGGAAAGCAGAGTTTTCATTAAATCCTTTTACCTTTTTTTTTTCTTGGTAATCCCCTAA AATAACAGTATGTGGGATATTGAATGTTAAAGGGATATTTTTTTCTATTATTTTTATAATTGTACAAAATTAAGCAAATGTTAAAAGTTTTATATGCTTT ATTAATGTTTTCAAAAGGTATTATACATGTGATACATTTTTTAAGCTTCAGTTGCTTGTCTTCTGGTACTTTCTGTTATGGGCTTTTGGGGAGCCAGAAG CCAATCTACAATCTCTTTTTGTTTGCCAGGACATGCAATAAAATTTAAAAAATAAATAAAAACTAATTAAGAAATTGTGTGTGTGTCTATCTGTGTCTGT TTGCCTATTTGTCCAT

TP73L.F10 (forward primer)
TP73L.R8 (reverse primer)
Single exon skipping

Frame 0

REKEREGLEFCYLLK*IHIVRVSGWGGWQNPGA RRKDSSIDQSYS*HVVPGKQCPDSI**ATVHEPG APEQHGPADSERLLVHQSL*HRPRAEQRHGALA LRTAQLHLRCSLSITRHPLQHRLPRPAQFRRVL PAVEHRQVGHLDVFH*TEETLLPNCKDMPHPDQG DDPTSSGSCYPRHACLQKS*ARHGGGEAVPQP* AEP*IQRGTDCPS*SFDSSRGEQPCPVCRRSHH RKTECAGTL*ATPGWH*IHDSLVQFHV*QQLCWR DEPPSNFNHCYSGNQRWASPGPTLL*GPDLCLP RKRQEGG*R*HQKAASFGQYKER*WYEAPVSSE HTWYPDDIHQETKIPR**TVILTSEGP*DL*NAV EDQRVPGTHAVPSSAHN*NVQATATAAAPALTS ETDLNTVSIFIW*QLPTSEQNEQHEQAAFCEPA YQPSAAQRPHSYNHS*WHGSQHSHDGHPHANGWR HEWTQPHPGTPSPTLHAIHLPLHTPTSVSHRLQ HCQFLSEVGLFIMSGLFHDPGADHHLSD*ALLH G*SGKSENP*AISTCDLEGHPGPPAAPRILLPFS SPADPKQCLYSQCGLQ*DPG*ACY*CCAIHPPP DHLFPTPR*VE*LQL*HGCSPQ*ATAHQRGGGV SLTM*ALPIPLLTASPLKALLLNLQSLLPSSSPS SCLIS*GKEK*EATSYLTSDLASNSDSGFKPSK L*LAEL*LPWLGRSEQKRVGCLLKLQRFLIDFY KACSPL*SKTIYINV*IYSIDFWVGGIEYCLKCN LNERKLSCTY*PFFNLLVLDGLSILLPLRGIMY GDRYLELNATCE*R*CTDSFSSLDSKYMPHQTF E*IHFHCLLCR*DCRYVFFSQCWYILYY*HFF** *WFTLG*FNPVIRRSSCPNVLFSFWLGMRKILK RPIAASSKTPDVMYLSISVTPLNLIPDTLSYNI DWENICCHYRGIKTKFHY*ID*LKYTFATVVRIL IDLIGMNAIYLVLTVKFYCLLIFRVNRNHSEML SLY*TVRYLNEP*IQLCKNLLKHR*YCLVNVSF VW*MFLLKTLLFYKTLHVEACLPFSL*GLQ*EW* FEKYKIMRLVFLWHKLHHCIIFFFNR*EFQFVG K*L*EPSFPSISLRDYP*T*KVPTEQEISLSWL LLLKPLKRRVPLKLWENMLMTIFQIFQKYNTFFC MHANEL*NLPMHSGQGLSLHISFHFNFKVQKGQ RGSKR*CVDCL*KVCIYFV*NCILCILIIFFFF LG*WDFQNHT*NLFLSFLYFHENTI**EYHIK*E IMLQF*EGREGKVFFYYFFKILYVKENESLISK FCCT*MVISTVNFCNKHAALQTH*GEE*KLFLG PSKKTNCFPYFAEGLNKPRTSELCQYYSGNTRAL EIPVLCLMDLALAKARHPYWLTSSWQPTLLECM SSQGKG*KDSKHRNH*KVGLMEFLWPQLNAVS* RIEKFLFGDVYKQKWKAEFSLNPFTFFFSW*SPK ITVCGILNVKGIFFSIIFIIVQN*ANVKSFICF INVFKRYYTCDTFFKLQLLVFWYFLLWAFGEPE ANLQSLFVCQDMQ*NLKNK*KLIKKLCVCLSVSV CLFVH

Frame 1

ERKRERDLSSVIFLSRFIL*GSRGGGVGKILEP EERTAALINLTANMLYLENNAQTQFSEPQYTNL GLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSP YAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSF QQSSTAKSATWTYSTELKKLYCQIAKTCPIQIK VMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHE LSREFNEGQIAPPSHLIRVEGNSHAQYVEDPIT GRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVG GMNRRPILIIVTLETRDGQVLGRRCFEARICACP GRDRKADEDSIRKQQVSDSTKNGDGTKRPFRQN THGIQMTSIKKRRSPDDELLYLPVRGRETYEML LKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQ KQTSIQSPSSYGNSSPPLNKMNSMNKLPSVSQL INPQQRNALTPTTIPDGMGANIPMMGTHMPMAG DMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCS IVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSM DDLASLKIPEQFRHAIWKGILDHRQLHEFSSPS HLLRTPSSASTVSVGSSETRGERVIDAVRFTLRQ TISFPPRDEWNDFNFDMDARRNKQQRIKEEGE* ASPCELFLSLS*LPAP*KHSCLIFKAFSLAPPL PLV*FLRGRRSKRLPLT*HLTWHLILILALSLQN YSLQNCSCHG*VEVSKKELGVSLSCRDFSLTFI KHVHPYSLRLYI*MYKYTV*IFGWGALSIV*NV I*MKEN*VALIDHFLIYLFWMACLYSFP*GVSCM VIGI*SLMLHVSDDDVQILSVLWILNTCHIKPL SRSISIAYYVGKTVDMYSFLSVGIFYITDISSS DDGSRWGDLIQL*EEVHVQTSSLVFGWE*GKFLK GP*QPVQKHPTSCI*AYQ*PP*I*YQIPYLTIL IGKTFAAITEVLKLNFTTRLTNSNTHLLLL*EF *LI*LG*MPSI*F*Q*SFTVY*YSG*IGIIQKC* VCTKQ*DISMNHKFNFVKIF*SIDNIVW*MFLL FGKCFF*RPSYSIKLCM*RLVYLSLSKVYNRSG DLKNIKL*DWFSCGINCITVSFSFLTGKSFSLLE SNCENPVSRPSPLGTTHRHERSPQSKR*VFHGC CCLNHLNEEFP*NFGKTC**QYSRSFRNITHFF ACMQMSSEIFPCILVKGCHCT*ASILILKCKRAS VALKGNVWIASEKCVYILCEIAYFVF*LFFFSS WDSGISRTTLETFFYRFCIFMKIPFSKNTTSNK K*CYNFKRGGKGKFFFIIFLKFCMLKRMSP*FQS FVVLKW**AL*TSATSMQLCKPIKGKNESCSLV LVRRQTASLTLLRV*INLGLPSYVSTIQVTLGP WKFLYCVSWIWH*PKRGTLTGLPPHGSLLSLSV* VARVRGKRIVSIETTRKWA*WSSCGLSSMQLAE ELKSFCLETFINRNGKQSFH*ILLPFFFLGNPL K*QYVGY*MLKGYFFLLFL*LYKIKQMLKVLYAL LMFSKGIIHVIHFLSFSCLSSGTFCYGLLGSQK PIYNLFLFARTCNKI*KINKN*LRNCVCVYLCL FAYLS

Frame 2

RERERGT*VLLSS*VDSYCKGLGVGGLAKSWSQ KKGQQH*SILQLTCCTWKTMPRLNLVSHSTRTW GS*TAWTSRFRTAPRPPVPITQTTRRTASRRPR PTHSPAPPSMLSLHHPPSPPTPTTQARTVSTCPS SSRAPPSRPPGRIPLN*RNSTAKLQRHAPSRSR **PHLLRELLSAPCLSTKKLSTSRRW*SGAPTM S*AVNSTRDRLPLLVI*FE*RGTAMPSM*KIPSQ EDRVCWYLMSHPRLALNSRQSCTISCVTAVVLE G*TAVQF*SLLLWKPEMGKSWADAALRPGSVLA QEETGRRMKIASESSKFRTVQRTVMVRSARFVRT HMVSR*HPSRNEDPQMMNCYTYQ*GAVRLMKCC *RSKSPWNSCSTFLSTQLKRTGNSNSSSTSTYF RNRPQYSLHLHMVTAPHL*TK*TA*TSCLL*ASL STLSSATPSLLQPFLMAWEPTFP*WAPTCQWLE T*MDSAPPRHSLPHSPCHPPPTAHPHLRIPQIA ALSVS*RGWAVHHVWTISRPRG*PPSIRLSITPW MIWQV*KSLSNFDMRSGRASWTTGSSTNSPPLL ISCGPQAVPLQSVWAPVRPGVSVLLMLCDSPSA RPSLSHPEMSGMTSTLTWMLAAISNSASKRRGSE PHHVSSSYPSPNCQPPKSTPA*SSKPSP*LLPF LLSDFLGEGEVRGYLLPNI*PGI*F*FWL*AFK TIACRTVAAMAR*K*AKKSWVSP*AAEISH*LL* SMFTLIV*DYIYKCINIQYRFLGGGH*VLFKM* FK*KKIELHLLTIF*FTCFGWLVYTPSLKGYHV W**VSRA*CYM*VTMMYRFFQFFGF*IHATSNL* VDPFPLLIM*VRL*ICILFSVLVYFILLTFLLV MMVHVGVI*SSYKKKFMSKRPL*FLVGNEENS* KAHSSQFKNTRRHVFEHISNPLKFNTRYLILQY* LGKHLLPLQRY*N*ISLLD*LTQIHICYCCKNS D*FDWDECHLSSSNSEVLLSINIQGK*ESFRNV ESVLNSKISQ*TINSTL*KSFEA*IILFGKCFFC LVNVSFKDPPIL*NSACRGLFTFLSLRFTIGVV I*KI*NYEIGFPVA*IASLYHFLF*PVRVSVCW KVTVRTQFPVHLP*GLPIDMKGPHRARDKSFMAA VA*TT*TKSSLETLGKHVNDNIPDLSEI*HIFL HACK*ALKSSHAFWSRAVIAHKLPF*F*SAKGP AWL*KVMCGLPLKSVYIFCVKLHTLYFDYFFFLL GIVGFPEPHLKPFFIVFVFS*KYHLVRIPHQIR NNATILRGEGRESFFLLFF*NFVC*RE*VLDFK VLLYLNGNKHCKLLQQACSFANPLRGRMKAVPWS **EDKLLPLLC*GFE*T*DFRAMSVLFR*H*GL GNSCTVSHGFGTSQSEAPLLAYLLMAAYSP*VY E*PG*GVKG**A*KPLESGLNGVLVASAQCS*LK N*KVFVWRRL*TEMESRVFIKSFYLFFFLVIP* NNSMWDIEC*RDIFFYYFYNCTKLSKC*KFYML Y*CFQKVLYM*YIF*ASVACLLVLSVMGFWGARS QSTISFCLPGHAIKFKK*IKTN*EIVCVSICVC LPICP

Sequence TP73L.aAug05

GCTTTTAGCCTCCCGGCTTTATATCTATATATACACAGGTATATGTGTATATTTTATATAATTGTTCTCCGTTCGTTGATATCAAAGACAGTTGAAGGAA ATGAATTTTGAAACTTCACGGTGTGCCACCCTACAGTACTGCCCTGACCCTTACATCCAGCGTTTCGTAGAAACCCCAGCTCATTTCTCTTGGAAAGAAA GTTATTACCGATCCACCATGTCCCAGAGCACACAGACAAATGAATTCCTCAGTCCAGAGGTTTTCCAGCATATCTGGGATTTTCTGGAACAGCCTATATG TTCAGTTCAGCCCATTGACTTGAACTTTGTGGATGAACCATCAGAAGATGGTGCGACAAACAAGATTGAGATTAGCATGGACTGTATCCGCATGCAGGAC TCGGACCTGAGTGACCCCATGTGGCCACAGTACACGAACCTGGGGCTCCTGAACAGCATGGACCAGCAGATTCAGAACGGCTCCTCGTCCACCAGTCCCT ATAACACAGACCACGCGCAGAACAGCGTCACGGCGCCCTCGCCCTACGCACAGCCCAGCTCCACCTTCGATGCTCTCTCTCCATCACCCGCCATCCCCTC CAACACCGACTACCCAGGCCCGCACAGTTTCGACGTGTCCTTCCAGCAGTCGAGCACCGCCAAGTCGGCCACCTGGACGTATTCCACTGAACTGAAGAAA CTCTACTGCCAAATTGCAAAGACATGCCCCATCCAGATCAAGGTGATGACCCCACCTCCTCAGGGAGCTGTTATCCGCGCCATGCCTGTCTACAAAAAAG CTGAGCACGTCACGGAGGTGGTGAAGCGGTGCCCCAACCATGAGCTGAGCCGTGAATTCAACGAGGGACAGATTGCCCCTCCTAGTCATTTGATTCGAGT AGAGGGGAACAGCCATGCCCAGTATGTAGAAGATCCCATCACAGGAAGACAGAGTGTGCTGGTACCTTATGAGCCACCCCAGGTTGGCACTGAATTCACG ACAGTCTTGTACAATTTCATGTGTAACAGCAGTTGTGTTGGAGGGATGAACCGCCGTCCAATTTTAATCATTGTTACTCTGGAAACCAGAGATGGGCAAG TCCTGGGCCGACGCTGCTTTGAGGCCCGGATCTGTGCTTGCCCAGGAAGAGACAGGAAGGCGGATGAAGATAGCATCAGAAAGCAGCAAGTTTCGGACAG TACAAAGAACGGTGATGGTACGAAGCGCCCGTTTCGTCAGAACACACATGGTATCCAGATGACATCCATCAAGAAACGAAGATCCCCAGATGATGAACTG TTATACTTACCAGTGAGGGGCCGTGAGACTTATGAAATGCTGTTGAAGATCAAAGAGTCCCTGGAACTCATGCAGTACCTTCCTCAGCACACAATTGAAA CGTACAGGCAACAGCAACAGCAGCAGCACCAGCACTTACTTCAGAAACAGACCTCAATACAGTCTCCATCTTCATATGGTAACAGCTCCCCACCTCTGAA CAAAATGAACAGCATGAACAAGCTGCCTTCTGTGAGCCAGCTTATCAACCCTCAGCAGCGCAACGCCCTCACTCCTACAACCATTCCTGATGGCATGGGA GCCAACATTCCCATGATGGGCACCCACATGCCAATGGCTGGAGACATGAATGGACTCAGCCCCACCCAGGCACTCCCTCCCCCACTCTCCATGCCATCCA CCTCCCACTGCACACCCCCACCTCCGTATCCCACAGATTGCAGCATTGTCAGTTTCTTAGCGAGGTTGGGCTGTTCATCATGTCTGGACTATTTCACGAC CCAGGGGCTGACCACCATCTATCAGATTGAGCATTACTCCATGGATGATCTGGCAAGTCTGAAAATCCCTGAGCAATTTCGACATGCGATCTGGAAGGGC ATCCTGGACCACCGGCAGCTCCACGAATTCTCCTCCCCTTCTCATCTCCTGCGGACCCCAAGCAGTGCCTCTACAGTCAGTGTGGGCTCCAGTGAGACCC GGGGTGAGCGTGTTATTGATGCTGTGCGATTCACCCTCCGCCAGACCATCTCTTTCCCACCCCGAGATGAGTGGAATGACTTCAACTTTGACATGGATGC TCGCCGCAATAAGCAACAGCGCATCAAAGAGGAGGGGGAGTGAGCCTCACCATGTGAGCTCTTCCTATCCCTCTCCTAACTGCCAGCCCCCTAAAAGCAC TCCTGCTTAATCTTCAAAGCCTTCTCCCTAGCTCCTCCCCTTCCTCTTGTCTGATTTCTTAGGGGAAGGAGAAGTAAGAGGCTACCTCTTACCTAACATC TGACCTGGCATCTAATTCTGATTCTGGCTTTAAGCCTTCAAAACTATAGCTTGCAGAACTGTAGCTGCCATGGCTAGGTAGAAGTGAGCAAAAAAGAGTT GGGTGTCTCCTTAAGCTGCAGAGATTTCTCATTGACTTTTATAAAGCATGTTCACCCTTATAGTCTAAGACTATATATATAAATGTATAAATATACAGTA TAGATTTTTGGGTGGGGGGCATTGAGTATTGTTTAAAATGTAATTTAAATGAAAGAAAATTGAGTTGCACTTATTGACCATTTTTTAATTTACTTGTTTT GGATGGCTTGTCTATACTCCTTCCCTTAAGGGGTATCATGTATGGTGATAGGTATCTAGAGCTTAATGCTACATGTGAGTGACGATGATGTACAGATTCT TTCAGTTCTTTGGATTCTAAATACATGCCACATCAAACCTTTGAGTAGATCCATTTCCATTGCTTATTATGTAGGTAAGACTGTAGATATGTATTCTTTT CTCAGTGTTGGTATATTTTATATTACTGACATTTCTTCTAGTGATGATGGTTCACGTTGGGGTGATTTAATCCAGTTATAAGAAGAAGTTCATGTCCAAA CGTCCTCTTTAGTTTTTGGTTGGGAATGAGGAAAATTCTTAAAAGGCCCATAGCAGCCAGTTCAAAAACACCCGACGTCATGTATTTGAGCATATCAGTA ACCCCCTTAAATTTAATACCAGATACCTTATCTTACAATATTGATTGGGAAAACATTTGCTGCCATTACAGAGGTATTAAAACTAAATTTCACTACTAGA TTGACTAACTCAAATACACATTTGCTACTGTTGTAAGAATTCTGATTGATTTGATTGGGATGAATGCCATCTATCTAGTTCTAACAGTGAAGTTTTACTG TCTATTAATATTCAGGGTAAATAGGAATCATTCAGAAATGTTGAGTCTGTACTAAACAGTAAGATATCTCAATGAACCATAAATTCAACTTTGTAAAAAT CTTTTGAAGCATAGATAATATTGTTTGGTAAATGTTTCTTTTGTTTGGTAAATGTTTCTTTTAAAGACCCTCCTATTCTATAAAACTCTGCATGTAGAGG CTTGTTTACCTTTCTCTCTCTAAGGTTTACAATAGGAGTGGTGATTTGAAAAATATAAAATTATGAGATTGGTTTTCCTGTGGCATAAATTGCATCACTG TATCATTTTCTTTTTTAACCGGTAAGAGTTTCAGTTTGTTGGAAAGTAACTGTGAGAACCCAGTTTCCCGTCCATCTCCCTTAGGGACTACCCATAGACA TGAAAGGTCCCCACAGAGCAAGAGATAAGTCTTTCATGGCTGCTGTTGCTTAAACCACTTAAACGAAGAGTTCCCTTGAAACTTTGGGAAAACATGTTAA TGACAATATTCCAGATCTTTCAGAAATATAACACATTTTTTTGCATGCATGCAAATGAGCTCTGAAATCTTCCCATGCATTCTGGTCAAGGGCTGTCATT GCACATAAGCTTCCATTTTAATTTTAAAGTGCAAAAGGGCCAGCGTGGCTCTAAAAGGTAATGTGTGGATTGCCTCTGAAAAGTGTGTATATATTTTGTG TGAAATTGCATACTTTGTATTTTGATTATTTTTTTTTTCTTCTTGGGATAGTGGGATTTCCAGAACCACACTTGAAACCTTTTTTTATCGTTTTTGTATT TTCATGAAAATACCATTTAGTAAGAATACCACATCAAATAAGAAATAATGCTACAATTTTAAGAGGGGAGGGAAGGGAAAGTTTTTTTTTATTATTTTTT TAAAATTTTGTATGTTAAAGAGAATGAGTCCTTGATTTCAAAGTTTTGTTGTACTTAAATGGTAATAAGCACTGTAAACTTCTGCAACAAGCATGCAGCT TTGCAAACCCATTAAGGGGAAGAATGAAAGCTGTTCCTTGGTCCTAGTAAGAAGACAAACTGCTTCCCTTACTTTGCTGAGGGTTTGAATAAACCTAGGA CTTCCGAGCTATGTCAGTACTATTCAGGTAACACTAGGGCCTTGGAAATTCCTGTACTGTGTCTCATGGATTTGGCACTAGCCAAAGCGAGGCACCCTTA CTGGCTTACCTCCTCATGGCAGCCTACTCTCCTTGAGTGTATGAGTAGCCAGGGTAAGGGGTAAAAGGATAGTAAGCATAGAAACCACTAGAAAGTGGGC TTAATGGAGTTCTTGTGGCCTCAGCTCAATGCAGTTAGCTGAAGAATTGAAAAGTTTTTGTTTGGAGACGTTTATAAACAGAAATGGAAAGCAGAGTTTT CATTAAATCCTTTTACCTTTTTTTTTTCTTGGTAATCCCCTAAAATAACAGTATGTGGGATATTGAATGTTAAAGGGATATTTTTTTCTATTATTTTTAT AATTGTACAAAATTAAGCAAATGTTAAAAGTTTTATATGCTTTATTAATGTTTTCAAAAGGTATTATACATGTGATACATTTTTTAAGCTTCAGTTGCTT GTCTTCTGGTACTTTCTGTTATGGGCTTTTGGGGAGCCAGAAGCCAATCTACAATCTCTTTTTGTTTGCCAGGACATGCAATAAAATTTAAAAAATAAAT AAAAACTAATTAAGAAATTGTGTGTGTGTCTATCTGTGTCTGTTTGCCTATTTGTCCAT

TP73L.F10 (forward primer)
TP73L.R8 (reverse primer)
Single exon skipping

Frame 0

AFSLPALYLYIHRYMCIFYIIVLRSLISKTVEG NEF*NFTVCHPTVLP*PLHPAFRRNPSSFLLERK LLPIHHVPEHTDK*IPQSRGFPAYLGFSGTAYM FSSAH*LELCG*TIRRWCDKQD*D*HGLYPHAG LGPE*PHVATVHEPGAPEQHGPADSERLLVHQSL *HRPRAEQRHGALALRTAQLHLRCSLSITRHPL QHRLPRPAQFRRVLPAVEHRQVGHLDVFH*TEE TLLPNCKDMPHPDQGDDPTSSGSCYPRHACLQKS *ARHGGGEAVPQP*AEP*IQRGTDCPS*SFDSS RGEQPCPVCRRSHHRKTECAGTL*ATPGWH*IH DSLVQFHV*QQLCWRDEPPSNFNHCYSGNQRWAS PGPTLL*GPDLCLPRKRQEGG*R*HQKAASFGQ YKER*WYEAPVSSEHTWYPDDIHQETKIPR**T VILTSEGP*DL*NAVEDQRVPGTHAVPSSAHN*N VQATATAAAPALTSETDLNTVSIFIW*QLPTSE QNEQHEQAAFCEPAYQPSAAQRPHSYNHS*WHG SQHSHDGHPHANGWRHEWTQPHPGTPSPTLHAIH LPLHTPTSVSHRLQHCQFLSEVGLFIMSGLFHD PGADHHLSD*ALLHG*SGKSENP*AISTCDLEG HPGPPAAPRILLPFSSPADPKQCLYSQCGLQ*DP G*ACY*CCAIHPPPDHLFPTPR*VE*LQL*HGC SPQ*ATAHQRGGGVSLTM*ALPIPLLTASPLKA LLLNLQSLLPSSSPSSCLIS*GKEK*EATSYLTS DLASNSDSGFKPSKL*LAEL*LPWLGRSEQKRV GCLLKLQRFLIDFYKACSPL*SKTIYINV*IYS IDFWVGGIEYCLKCNLNERKLSCTY*PFFNLLVL DGLSILLPLRGIMYGDRYLELNATCE*R*CTDS FSSLDSKYMPHQTFE*IHFHCLLCR*DCRYVFF SQCWYILYY*HFF***WFTLG*FNPVIRRSSCPN VLFSFWLGMRKILKRPIAASSKTPDVMYLSISV TPLNLIPDTLSYNIDWENICCHYRGIKTKFHY* ID*LKYTFATVVRILIDLIGMNAIYLVLTVKFYC LLIFRVNRNHSEMLSLY*TVRYLNEP*IQLCKN LLKHR*YCLVNVSFVW*MFLLKTLLFYKTLHVE ACLPFSL*GLQ*EW*FEKYKIMRLVFLWHKLHHC IIFFFNR*EFQFVGK*L*EPSFPSISLRDYP*T *KVPTEQEISLSWLLLLKPLKRRVPLKLWENML MTIFQIFQKYNTFFCMHANEL*NLPMHSGQGLSL HISFHFNFKVQKGQRGSKR*CVDCL*KVCIYFV *NCILCILIIFFFFLG*WDFQNHT*NLFLSFLY FHENTI**EYHIK*EIMLQF*EGREGKVFFYYFF KILYVKENESLISKFCCT*MVISTVNFCNKHAA LQTH*GEE*KLFLGPSKKTNCFPYFAEGLNKPR TSELCQYYSGNTRALEIPVLCLMDLALAKARHPY WLTSSWQPTLLECMSSQGKG*KDSKHRNH*KVG LMEFLWPQLNAVS*RIEKFLFGDVYKQKWKAEF SLNPFTFFFSW*SPKITVCGILNVKGIFFSIIFI IVQN*ANVKSFICFINVFKRYYTCDTFFKLQLL VFWYFLLWAFGEPEANLQSLFVCQDMQ*NLKNK *KLIKKLCVCLSVSVCLFVH

Frame 1

LLASRLYIYIYTGICVYFI*LFSVR*YQRQLKE MNFETSRCATLQYCPDPYIQRFVETPAHFSWKE SYYRSTMSQSTQTNEFLSPEVFQHIWDFLEQPIC SVQPIDLNFVDEPSEDGATNKIEISMDCIRMQD SDLSDPMWPQYTNLGLLNSMDQQIQNGSSSTSP YNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPS NTDYPGPHSFDVSFQQSSTAKSATWTYSTELKK LYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKK AEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRV EGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFT TVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQ VLGRRCFEARICACPGRDRKADEDSIRKQQVSDS TKNGDGTKRPFRQNTHGIQMTSIKKRRSPDDEL LYLPVRGRETYEMLLKIKESLELMQYLPQHTIE TYRQQQQQQHQHLLQKQTSIQSPSSYGNSSPPLN KMNSMNKLPSVSQLINPQQRNALTPTTIPDGMG ANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPS TSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTT QGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKG ILDHRQLHEFSSPSHLLRTPSSASTVSVGSSET RGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDA RRNKQQRIKEEGE*ASPCELFLSLS*LPAP*KH SCLIFKAFSLAPPLPLV*FLRGRRSKRLPLT*H LTWHLILILALSLQNYSLQNCSCHG*VEVSKKEL GVSLSCRDFSLTFIKHVHPYSLRLYI*MYKYTV *IFGWGALSIV*NVI*MKEN*VALIDHFLIYLF WMACLYSFP*GVSCMVIGI*SLMLHVSDDDVQIL SVLWILNTCHIKPLSRSISIAYYVGKTVDMYSF LSVGIFYITDISSSDDGSRWGDLIQL*EEVHVQ TSSLVFGWE*GKFLKGP*QPVQKHPTSCI*AYQ* PP*I*YQIPYLTILIGKTFAAITEVLKLNFTTR LTNSNTHLLLL*EF*LI*LG*MPSI*F*Q*SFT VY*YSG*IGIIQKC*VCTKQ*DISMNHKFNFVKI F*SIDNIVW*MFLLFGKCFF*RPSYSIKLCM*R LVYLSLSKVYNRSGDLKNIKL*DWFSCGINCIT VSFSFLTGKSFSLLESNCENPVSRPSPLGTTHRH ERSPQSKR*VFHGCCCLNHLNEEFP*NFGKTC* *QYSRSFRNITHFFACMQMSSEIFPCILVKGCH CT*ASILILKCKRASVALKGNVWIASEKCVYILC EIAYFVF*LFFFSSWDSGISRTTLETFFYRFCI FMKIPFSKNTTSNKK*CYNFKRGGKGKFFFIIF LKFCMLKRMSP*FQSFVVLKW**AL*TSATSMQL CKPIKGKNESCSLVLVRRQTASLTLLRV*INLG LPSYVSTIQVTLGPWKFLYCVSWIWH*PKRGTL TGLPPHGSLLSLSV*VARVRGKRIVSIETTRKWA *WSSCGLSSMQLAEELKSFCLETFINRNGKQSF H*ILLPFFFLGNPLK*QYVGY*MLKGYFFLLFL *LYKIKQMLKVLYALLMFSKGIIHVIHFLSFSCL SSGTFCYGLLGSQKPIYNLFLFARTCNKI*KIN KN*LRNCVCVYLCLFAYLS

Frame 2

F*PPGFISIYTQVYVYILYNCSPFVDIKDS*RK *ILKLHGVPPYSTALTLTSSVS*KPQLISLGKK VITDPPCPRAHRQMNSSVQRFSSISGIFWNSLY VQFSPLT*TLWMNHQKMVRQTRLRLAWTVSACRT RT*VTPCGHSTRTWGS*TAWTSRFRTAPRPPVP ITQTTRRTASRRPRPTHSPAPPSMLSLHHPPSP PTPTTQARTVSTCPSSSRAPPSRPPGRIPLN*RN STAKLQRHAPSRSR**PHLLRELLSAPCLSTKK LSTSRRW*SGAPTMS*AVNSTRDRLPLLVI*FE *RGTAMPSM*KIPSQEDRVCWYLMSHPRLALNSR QSCTISCVTAVVLEG*TAVQF*SLLLWKPEMGK SWADAALRPGSVLAQEETGRRMKIASESSKFRT VQRTVMVRSARFVRTHMVSR*HPSRNEDPQMMNC YTYQ*GAVRLMKCC*RSKSPWNSCSTFLSTQLK RTGNSNSSSTSTYFRNRPQYSLHLHMVTAPHL* TK*TA*TSCLL*ASLSTLSSATPSLLQPFLMAWE PTFP*WAPTCQWLET*MDSAPPRHSLPHSPCHP PPTAHPHLRIPQIAALSVS*RGWAVHHVWTISR PRG*PPSIRLSITPWMIWQV*KSLSNFDMRSGRA SWTTGSSTNSPPLLISCGPQAVPLQSVWAPVRP GVSVLLMLCDSPSARPSLSHPEMSGMTSTLTWM LAAISNSASKRRGSEPHHVSSSYPSPNCQPPKST PA*SSKPSP*LLPFLLSDFLGEGEVRGYLLPNI *PGI*F*FWL*AFKTIACRTVAAMAR*K*AKKS WVSP*AAEISH*LL*SMFTLIV*DYIYKCINIQY RFLGGGH*VLFKM*FK*KKIELHLLTIF*FTCF GWLVYTPSLKGYHVW**VSRA*CYM*VTMMYRF FQFFGF*IHATSNL*VDPFPLLIM*VRL*ICILF SVLVYFILLTFLLVMMVHVGVI*SSYKKKFMSK RPL*FLVGNEENS*KAHSSQFKNTRRHVFEHIS NPLKFNTRYLILQY*LGKHLLPLQRY*N*ISLLD *LTQIHICYCCKNSD*FDWDECHLSSSNSEVLL SINIQGK*ESFRNVESVLNSKISQ*TINSTL*K SFEA*IILFGKCFFCLVNVSFKDPPIL*NSACRG LFTFLSLRFTIGVVI*KI*NYEIGFPVA*IASL YHFLF*PVRVSVCWKVTVRTQFPVHLP*GLPID MKGPHRARDKSFMAAVA*TT*TKSSLETLGKHVN DNIPDLSEI*HIFLHACK*ALKSSHAFWSRAVI AHKLPF*F*SAKGPAWL*KVMCGLPLKSVYIFC VKLHTLYFDYFFFLLGIVGFPEPHLKPFFIVFVF S*KYHLVRIPHQIRNNATILRGEGRESFFLLFF *NFVC*RE*VLDFKVLLYLNGNKHCKLLQQACS FANPLRGRMKAVPWS**EDKLLPLLC*GFE*T*D FRAMSVLFR*H*GLGNSCTVSHGFGTSQSEAPL LAYLLMAAYSP*VYE*PG*GVKG**A*KPLESG LNGVLVASAQCS*LKN*KVFVWRRL*TEMESRVF IKSFYLFFFLVIP*NNSMWDIEC*RDIFFYYFY NCTKLSKC*KFYMLY*CFQKVLYM*YIF*ASVA CLLVLSVMGFWGARSQSTISFCLPGHAIKFKK*I KTN*EIVCVSICVCLPICP