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Sequences

Sequence DNMT3B.iAug05

ATTGAAGAATGGGGCCCATTTGACTTGGTGATTGGCGGAAGCCCATGCAACGATCTCTCAAATGTGAATCCAGCCAGGAAAGGCCTGTATGAGGGTACAG
GCCGGCTCTTCTTCGAATTTTACCACCTGCTGAATTACTCACGCCCCAAGGAGGGTGATGACCGGCCGTTCTTCTGGATGTTTGAGAATGTTGTAGCCAT
GAAGGTTGGCGACAAGAGGGACATCTCACGGTTCCTGGAGTGTAATCCAGTGATGATTGATGCCATCAAAGTTTCTGCTGCTCACAGGGCCCGATACTTC
TGGGGCAACCTACCCGGGATGAACAGGCCCGTGATAGCATCAAAGAATGATAAACTCGAGCTGCAGGACTGCTTGGAATACAATAGGATAGCCAAGGATC
TTTGGCTTTCCTGTGCACTACACAGACGTGTCCAACATGGGCCGTGGTGCCCGCCAGAAGCTGCTGGGAAGGTCCTGGAGCGTGCCTGTCATCCGACACC
TCTTCGCCCCTCTGAAGGACTACTTTGCATGTGAATAGTTCCAGCCAGGCCCCAAGCCCACTGGGGTGTGTGGCAGAGCCAGGACCCAGGAGGTGTGATT
CCTGAAGGCATCCCCAGGCCCTGCTCTTCCTCAGCTGTGTGGGTCATACCGTGTACCTCAGTTCCCTCTTGCTCAGTGGGGGCAGAGCCACCTGACTCTT
GCAGGGGTAGCCTGAGGTGCCGCCTCCTTGTGCACAAATCAGACCTGGCTGCTTGGAGCAGCCTAACACGGTGCTCATTTTTTCTTCTCCTAAAACTTTA
AAACTTGAAGTAGGTAGCAACGTGGCTTTTTTTTTTTCCCTTCCTGGGTCTACCACTCAGAGAAACAATGGCTAAGATACCAAAACCACAGTGCCGACAG
CTCTCCAATACTCAGGTTAATGCTGAAAAATCATCCAAGACAGTTATTGCAAGAGTTTAATTTTTGAAAACTGGCTACTGCTCTGTGTTTACAGACGTGT
GCAGTTGTAGGCATGTAGCTACAGGACATTTTTAAGGGCCCAGGATCGTTTTTTCCCAGGGCAAGCAGAAGAGAAAATGTTGTATATGTCTTTTACCCGG
CACATTCCCCTTGCCTAAATACAAGGGCTGGAGTCTGCACGGGACCTATTAGAGTATTTTCCACAATGATGATGATTTCAGCAGGGATGACGTCATCATC
ACATTCAGGGCTATTTTTTCCCCCACAAACCCAAGGGCAGGGGCCACTCTTAGCTAAATCCCTCCCCGTGACTGCAATAGAACCCTCTGGGGAGCTCAGG
AAGGGGTGTGCTGAGTTCTATAATATAAGCTGCCATATATTTTGTAGACAAGTATGGCTCCTCCATATCTCCCTCTTCCCTAGGAGAGGAGTGTGAAGCA
AGGAGCTTAGATAAGACACCCCCTCAAACCCATTCCCTCTCCAGGAGACCTACCCTCCACAGGCACAGGTCCCCAGATGAGAAGTCTGCTACCCTCATTT
CTCATCTTTTTACTAAACTCAGAGGCAGTGACAGCAGTCAGGGACAGACATACATTTCTCATACCTTCCCCACATCTGAGAGATGACAGGGAAAACTGCA
AAGCTCGGTGCTCCCTTTGGAGATTTTTTAATCCTTTTTTATTCCATAAGAAGTCGTTTTTAGGGAGAACGGGAATTCAGACAAGCTGCATTTCAGAAAT
GCTGTCATAATGGTTTTTAACACCTTTTACTCTTCTTACTGGTGCTATTTTGTAGAATAAGGAACAACGTTGACAAGTTTTGTGGGGCTTTTTATACACT
TTTTAAAATCTCAAACTTCTATTTTTATGTTTAACGTTTTCATTAAAATTTTTTTTGTAACTGGAGCCACGACGTAACAAATATGGGGAAAAAACTGTGC
CTTGTTTCAACAGTTTTTGCTAATTTTTAGGCTGAAAGATGACGGATGCCTAGAGTTTACCTTATGTTTAATTAAAATCAGTATTTGTCTATAA
  • DNMT3B.u.f.26 (forward primer)
  • DNMT3B.u.r.28 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

IEEWGPFDLVIGGSPCNDLSNVNPARKGLYEGT
GRLFFEFYHLLNYSRPKEGDDRPFFWMFENVVAM
KVGDKRDISRFLECNPVMIDAIKVSAAHRARYF
WGNLPGMNRPVIASKNDKLELQDCLEYNRIAKD
LWLSCALHRRVQHGPWCPPEAAGKVLERACHPTP
LRPSEGLLCM*IVPARPQAHWGVWQSQDPGGVI
PEGIPRPCSSSAVWVIPCTSVPSCSVGAEPPDS
CRGSLRCRLLVHKSDLAAWSSLTRCSFFLLLKL*
NLK*VATWLFFFPFLGLPLRETMAKIPKPQCRQ
LSNTQVNAEKSSKTVIARV*FLKTGYCSVFTDV
CSCRHVATGHF*GPRIVFSQGKQKRKCCICLLPG
TFPLPKYKGWSLHGTY*SIFHNDDDFSRDDVII
TFRAIFSPTNPRAGATLS*IPPRDCNRTLWGAQ
EGVC*VL*YKLPYIL*TSMAPPYLPLP*ERSVKQ
GA*IRHPLKPIPSPGDLPSTGTGPQMRSLLPSF
LIFLLNSEAVTAVRDRHTFLIPSPHLRDDRENC
KARCSLWRFFNPFLFHKKSFLGRTGIQTSCISEM
LS*WFLTPFTLLTGAIL*NKEQR*QVLWGFLYT
F*NLKLLFLCLTFSLKFFL*LEPRRNKYGEKTV
PCFNSFC*FLG*KMTDA*SLPYV*LKSVFVY

Frame 1

LKNGAHLTW*LAEAHATISQM*IQPGKACMRVQ
AGSSSNFTTC*ITHAPRRVMTGRSSGCLRML*P
*RLATRGTSHGSWSVIQ**LMPSKFLLLTGPDTS
GATYPG*TGP**HQRMINSSCRTAWNTIG*PRI
FGFPVHYTDVSNMGRGARQKLLGRSWSVPVIRH
LFAPLKDYFACE*FQPGPKPTGVCGRARTQEV*F
LKASPGPALPQLCGSYRVPQFPLAQWGQSHLTL
AGVA*GAASLCTNQTWLLGAA*HGAHFFFS*NF
KT*SR*QRGFFFFPSWVYHSEKQWLRYQNHSADS
SPILRLMLKNHPRQLLQEFNF*KLATALCLQTC
AVVGM*LQDIFKGPGSFFPRASRRENVVYVFYP
AHSPCLNTRAGVCTGPIRVFSTMMMISAGMTSSS
HSGLFFPPQTQGQGPLLAKSLPVTAIEPSGELR
KGCAEFYNISCHIFCRQVWLLHISLFPRRGV*S
KELR*DTPSNPFPLQETYPPQAQVPR*EVCYPHF
SSFY*TQRQ*QQSGTDIHFSYLPHI*EMTGKTA
KLGAPFGDFLILFYSIRSRF*GEREFRQAAFQK
CCHNGF*HLLLFLLVLFCRIRNNVDKFCGAFYTL
FKISNFYFYV*RFH*NFFCNWSHDVTNMGKKLC
LVSTVFANF*AER*RMPRVYLMFN*NQYLSI

Frame 2

*RMGPI*LGDWRKPMQRSLKCESSQERPV*GYR
PALLRILPPAELLTPQGG**PAVLLDV*ECCSH
EGWRQEGHLTVPGV*SSDD*CHQSFCCSQGPIL
LGQPTRDEQARDSIKE**TRAAGLLGIQ*DSQGS
LAFLCTTQTCPTWAVVPARSCWEGPGACLSSDT
SSPL*RTTLHVNSSSQAPSPLGCVAEPGPRRCD
S*RHPQALLFLSCVGHTVYLSSLLLSGGRAT*LL
QG*PEVPPPCAQIRPGCLEQPNTVLIFSSPKTL
KLEVGSNVAFFFSLPGSTTQRNNG*DTKTTVPT
ALQYSG*C*KIIQDSYCKSLIFENWLLLCVYRRV
QL*ACSYRTFLRAQDRFFPGQAEEKMLYMSFTR
HIPLA*IQGLESARDLLEYFPQ***FQQG*RHH
HIQGYFFPHKPKGRGHS*LNPSP*LQ*NPLGSSG
RGVLSSII*AAIYFVDKYGSSISPSSLGEECEA
RSLDKTPPQTHSLSRRPTLHRHRSPDEKSATLI
SHLFTKLRGSDSSQGQTYISHTFPTSER*QGKLQ
SSVLPLEIF*SFFIP*EVVFRENGNSDKLHFRN
AVIMVFNTFYSSYWCYFVE*GTTLTSFVGLFIH
FLKSQTSIFMFNVFIKIFFVTGATT*QIWGKNCA
LFQQFLLIFRLKDDGCLEFTLCLIKISICL*

Sequence DNMT3B.kAug05

ATTGAAGAATGGGGCCCATTTGACTTGGTGATTGGCGGAAGCCCATGCAACGATCTCTCAAATGTGAATCCAGCCAGGAAAGGCCTGTATGAGGGTACAG
GCCGGCTCTTCTTCGAATTTTACCACCTGCTGAATTACTCACGCCCCAAGGAGGGTGATGACCGGCCGTTCTTCTGGATGTTTGAGAATGTTGTAGCCAT
GAAGGTTGGCGACAAGAGGGACATCTCACGGTTCCTGGAGTGTAATCCAGTGATGATTGATGCCATCAAAGTTTCTGCTGCTCACAGGGCCCGATACTTC
TGGGGCAACCTACCCGGGATGAACAGTTAAAGAAAGTACAGACAATAACCACCAAGTCGAACTCGATCAAACAGGGGAAAAACCAACTTTTCCCTGTTGT
CATGAATGGCAAAGAAGATGTTTTGTGGTGCACTGAGCTCGAAAGGATCTTTGGCTTTCCTGTGCACTACACAGACGTGTCCAACATGGGCCGTGGTGCC
CGCCAGAAGCTGCTGGGAAGGTCCTGGAGCGTGCCTGTCATCCGACACCTCTTCGCCCCTCTGAAGGACTACTTTGCATGTGAATAGTTCCAGCCAGGCC
CCAAGCCCACTGGGGTGTGTGGCAGAGCCAGGACCCAGGAGGTGTGATTCCTGAAGGCATCCCCAGGCCCTGCTCTTCCTCAGCTGTGTGGGTCATACCG
TGTACCTCAGTTCCCTCTTGCTCAGTGGGGGCAGAGCCACCTGACTCTTGCAGGGGTAGCCTGAGGTGCCGCCTCCTTGTGCACAAATCAGACCTGGCTG
CTTGGAGCAGCCTAACACGGTGCTCATTTTTTCTTCTCCTAAAACTTTAAAACTTGAAGTAGGTAGCAACGTGGCTTTTTTTTTTTCCCTTCCTGGGTCT
ACCACTCAGAGAAACAATGGCTAAGATACCAAAACCACAGTGCCGACAGCTCTCCAATACTCAGGTTAATGCTGAAAAATCATCCAAGACAGTTATTGCA
AGAGTTTAATTTTTGAAAACTGGCTACTGCTCTGTGTTTACAGACGTGTGCAGTTGTAGGCATGTAGCTACAGGACATTTTTAAGGGCCCAGGATCGTTT
TTTCCCAGGGCAAGCAGAAGAGAAAATGTTGTATATGTCTTTTACCCGGCACATTCCCCTTGCCTAAATACAAGGGCTGGAGTCTGCACGGGACCTATTA
GAGTATTTTCCACAATGATGATGATTTCAGCAGGGATGACGTCATCATCACATTCAGGGCTATTTTTTCCCCCACAAACCCAAGGGCAGGGGCCACTCTT
AGCTAAATCCCTCCCCGTGACTGCAATAGAACCCTCTGGGGAGCTCAGGAAGGGGTGTGCTGAGTTCTATAATATAAGCTGCCATATATTTTGTAGACAA
GTATGGCTCCTCCATATCTCCCTCTTCCCTAGGAGAGGAGTGTGAAGCAAGGAGCTTAGATAAGACACCCCCTCAAACCCATTCCCTCTCCAGGAGACCT
ACCCTCCACAGGCACAGGTCCCCAGATGAGAAGTCTGCTACCCTCATTTCTCATCTTTTTACTAAACTCAGAGGCAGTGACAGCAGTCAGGGACAGACAT
ACATTTCTCATACCTTCCCCACATCTGAGAGATGACAGGGAAAACTGCAAAGCTCGGTGCTCCCTTTGGAGATTTTTTAATCCTTTTTTATTCCATAAGA
AGTCGTTTTTAGGGAGAACGGGAATTCAGACAAGCTGCATTTCAGAAATGCTGTCATAATGGTTTTTAACACCTTTTACTCTTCTTACTGGTGCTATTTT
GTAGAATAAGGAACAACGTTGACAAGTTTTGTGGGGCTTTTTATACACTTTTTAAAATCTCAAACTTCTATTTTTATGTTTAACGTTTTCATTAAAATTT
TTTTTGTAACTGGAGCCACGACGTAACAAATATGGGGAAAAAACTGTGCCTTGTTTCAACAGTTTTTGCTAATTTTTAGGCTGAAAGATGACGGATGCCT
AGAGTTTACCTTATGTTTAATTAAAATCAGTATTTGTCTATAA
  • DNMT3B.u.f.26 (forward primer)
  • DNMT3B.u.r.28 (reverse primer)
  • Mutually exclusive exon skipping

Frame 0

IEEWGPFDLVIGGSPCNDLSNVNPARKGLYEGT
GRLFFEFYHLLNYSRPKEGDDRPFFWMFENVVAM
KVGDKRDISRFLECNPVMIDAIKVSAAHRARYF
WGNLPGMNS*RKYRQ*PPSRTRSNRGKTNFSLL
S*MAKKMFCGALSSKGSLAFLCTTQTCPTWAVVP
ARSCWEGPGACLSSDTSSPL*RTTLHVNSSSQA
PSPLGCVAEPGPRRCDS*RHPQALLFLSCVGHT
VYLSSLLLSGGRAT*LLQG*PEVPPPCAQIRPGC
LEQPNTVLIFSSPKTLKLEVGSNVAFFFSLPGS
TTQRNNG*DTKTTVPTALQYSG*C*KIIQDSYC
KSLIFENWLLLCVYRRVQL*ACSYRTFLRAQDRF
FPGQAEEKMLYMSFTRHIPLA*IQGLESARDLL
EYFPQ***FQQG*RHHHIQGYFFPHKPKGRGHS
*LNPSP*LQ*NPLGSSGRGVLSSII*AAIYFVDK
YGSSISPSSLGEECEARSLDKTPPQTHSLSRRP
TLHRHRSPDEKSATLISHLFTKLRGSDSSQGQT
YISHTFPTSER*QGKLQSSVLPLEIF*SFFIP*E
VVFRENGNSDKLHFRNAVIMVFNTFYSSYWCYF
VE*GTTLTSFVGLFIHFLKSQTSIFMFNVFIKI
FFVTGATT*QIWGKNCALFQQFLLIFRLKDDGCL
EFTLCLIKISICL*

Frame 1

LKNGAHLTW*LAEAHATISQM*IQPGKACMRVQ
AGSSSNFTTC*ITHAPRRVMTGRSSGCLRML*P
*RLATRGTSHGSWSVIQ**LMPSKFLLLTGPDTS
GATYPG*TVKESTDNNHQVELDQTGEKPTFPCC
HEWQRRCFVVH*ARKDLWLSCALHRRVQHGPWC
PPEAAGKVLERACHPTPLRPSEGLLCM*IVPARP
QAHWGVWQSQDPGGVIPEGIPRPCSSSAVWVIP
CTSVPSCSVGAEPPDSCRGSLRCRLLVHKSDLA
AWSSLTRCSFFLLLKL*NLK*VATWLFFFPFLGL
PLRETMAKIPKPQCRQLSNTQVNAEKSSKTVIA
RV*FLKTGYCSVFTDVCSCRHVATGHF*GPRIV
FSQGKQKRKCCICLLPGTFPLPKYKGWSLHGTY*
SIFHNDDDFSRDDVIITFRAIFSPTNPRAGATL
S*IPPRDCNRTLWGAQEGVC*VL*YKLPYIL*T
SMAPPYLPLP*ERSVKQGA*IRHPLKPIPSPGDL
PSTGTGPQMRSLLPSFLIFLLNSEAVTAVRDRH
TFLIPSPHLRDDRENCKARCSLWRFFNPFLFHK
KSFLGRTGIQTSCISEMLS*WFLTPFTLLTGAIL
*NKEQR*QVLWGFLYTF*NLKLLFLCLTFSLKF
FL*LEPRRNKYGEKTVPCFNSFC*FLG*KMTDA
*SLPYV*LKSVFVY

Frame 2

*RMGPI*LGDWRKPMQRSLKCESSQERPV*GYR
PALLRILPPAELLTPQGG**PAVLLDV*ECCSH
EGWRQEGHLTVPGV*SSDD*CHQSFCCSQGPIL
LGQPTRDEQLKKVQTITTKSNSIKQGKNQLFPVV
MNGKEDVLWCTELERIFGFPVHYTDVSNMGRGA
RQKLLGRSWSVPVIRHLFAPLKDYFACE*FQPG
PKPTGVCGRARTQEV*FLKASPGPALPQLCGSYR
VPQFPLAQWGQSHLTLAGVA*GAASLCTNQTWL
LGAA*HGAHFFFS*NFKT*SR*QRGFFFFPSWV
YHSEKQWLRYQNHSADSSPILRLMLKNHPRQLLQ
EFNF*KLATALCLQTCAVVGM*LQDIFKGPGSF
FPRASRRENVVYVFYPAHSPCLNTRAGVCTGPI
RVFSTMMMISAGMTSSSHSGLFFPPQTQGQGPLL
AKSLPVTAIEPSGELRKGCAEFYNISCHIFCRQ
VWLLHISLFPRRGV*SKELR*DTPSNPFPLQET
YPPQAQVPR*EVCYPHFSSFY*TQRQ*QQSGTDI
HFSYLPHI*EMTGKTAKLGAPFGDFLILFYSIR
SRF*GEREFRQAAFQKCCHNGF*HLLLFLLVLF
CRIRNNVDKFCGAFYTLFKISNFYFYV*RFH*NF
FCNWSHDVTNMGKKLCLVSTVFANF*AER*RMP
RVYLMFN*NQYLSI

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