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Sequences

Sequence DNMT3B.fAug05

ACCCACTCCCGCTGCCCCGTCCGGCCCGCGCCGCTTCCTCGCAGCAGCTGCTCCCGGCTCCGCGGCCGCAGCCCGCGTGGACGCTCCGAGCGCCCCCCGA CGGACGGGACCGGCTCCCTGGCGGTCGGGCGAGCGGGCGGCAACGCTGCCCGGCCGGCAGCGCTGGGGTTAAGTGGCCCAAGTAAACCTAGCTCGGCGAT CGGCGCCGGAGATTCGCGAGCCCAGCGCCCTGCACGGCCGCCAGCCGGCCTCCCGCCAGCCAGCCCCGACCCGCGGCTCCGCCGCCCAGCCGCGCCCCAG CCAGCCCTGCGGCAGGAAAGCATGAAGGGAGACACCAGGCATCTCAATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCGATCCTCGTCAACGGGGCCT GCAGCGACCAGTCCTCCGACTCGCCCCCAATCCTGGAGGCTATCCGCACCCCGGAGATCAGAGGCCGAAGATCAAGCTCGCGACTCTCCAAGAGGGAGGT GTCCAGTCTGCTAAGCTACACACAGGACTTGACAGGCGATGGCGACGGGGAAGATGGGGATGGCTCTGACACCCCAGTCATGCCAAAGCTCTTCCGGGAA ACCAGGACTCGTTCAGAAAGCCCAGCTGTCCGAACTCGAAATAACAACAGTGTCTCCAGCCGGGAGAGGCACAGGCCTTCCCCACGTTCCACCCGAGGCC GGCAGGGCCGCAACCATGTGGACGAGTCCCCCGTGGAGTTCCCGGCTACCAGGTCCCTGAGACGGCGGGCAACAGCATCGGCAGGAACGCCATGGCCGTC CCCTCCCAGCTCTTACCTTACCATCGACCTCACAGACGACACAGAGGACACACATGGGACGCCCCAGAGCAGCAGTACCCCCTACGCCCGCCTAGCCCAG GACAGCCAGCAGGGGGGCATGGAGTCCCCGCAGGTGGAGGCAGACAGTGGAGATGGAGACAGTTCAGAGTATCAGGATGGGAAGGAGTTTGGAATAGGGG ACCTCGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCCGCCATGGTGGTGTCTTGGAAGGCCACCTCCAAGCGACAGGCTATGTCTGGCATGCGGTG GGTCCAGTGGTTTGGCGATGGCAAGTTCTCCGAGGTCTCTGCAGACAAACTGGTGGCACTGGGGCTGTTCAGCCAGCACTTTAATTTGGCCACCTTCAAT AAGCTCGTCTCCTATCGAAAAGCCATGTACCATGCTCTGGAGAAAGCTAGGGTGCGAGCTGGCAAGACCTTCCCCAGCAGCCCTGGAGACTCATTGGAGG ACCAGCTGAAGCCCATGTTGGAGTGGGCCCACGGGGGCTTCAAGCCCACTGGGATCGAGGGCCTCAAACCCAACAACACGCAACCAGAGAACAAGACTCG AAGACGCACAGCTGACGACTCAGCCACCTCTGACTACTGCCCCGCACCCAAGCGCCTCAAGACAAATTGCTATAACAACGGCAAAGACCGAGGGGATGAA GATCAGAGCCGAGAACAAATGGCTTCAGATGTTGCCAACAACAAGAGCAGCCTGGAAGATGGCTGTTTGTCTTGTGGCAGGAAAAACCCCGTGTCCTTCC ACCCTCTCTTTGAGGGGGGGCTCTGTCAGACATGCCGGCACCTGTGTGGAGACTCAGTGAAGCCCACTCATCCCAGCTGCCTTGCACTCTGCCTCTGGGG CAGGATGGGGGACAGCTGTCACAGAGGCCAATGGCACGCAGGTCACAGCCAGTTGTCCTTTTAAAGGACACCAAGCCACCAGCAGTGTGTTCTGCAGCTG TATCCTAGGGCCTCCACCAGCAAGCCGGCAGGGCCTGCCCTTCTCTGGTCTCCGATTTCACTGGTGTTTCTCTCTGGCTGCCAGGATCGCTTCCTTGAGC TGTTTTACATGTATGATGACGATGGCTATCAGTCTTACTGCACTGTGTGCTGCGAGGGCCGAGAGCTGCTGCTTTGCAGCAACACGAGCTGCTGCCGGTG TTTCTGTGTGGAGTGCCTGGAGGTGCTGGTGGGCACAGGCACAGCGGCCGAGGCCAAGCTTCAGGAGCCCTGGAGCTGTTACATGTGTCTCCCGCAGCGC TGTCATGGCGTCCTGCGGCGCCGGAAGGACTGGAACGTGCGCCTGCAGGCCTTCTTCACCAGTGACACGGGGCTTGAATATGAAGCCCCCAAGCTGTACC CTGCCATTCCCGCAGCCCGAAGGCGGCCCATTCGAGTCCTGTCATTGTTTGATGGCATCGCGACAGGCTACCTAGTCCTCAAAGAGTTGGGCATAAAGGT AGGAAAGTACGTCGCTTCTGAAGTGTGTGAGGAGTCCATTGCTGTTGGAACCGTGAAGCACGAGGGGAATATCAAATACGTGAACGACGTGAGGAACATC ACAAAGAAAAATATTGAAGAATGGGGCCCATTTGACTTGGTGATTGGCGGAAGCCCATGCAACGATCTCTCAAATGTGAATCCAGCCAGGAAAGGCCTGT ATGAGGGTACAGGCCGGCTCTTCTTCGAATTTTACCACCTGCTGAATTACTCACGCCCCAAGGAGGGTGATGACCGGCCGTTCTTCTGGATGTTTGAGAA TGTTGTAGCCATGAAGGTTGGCGACAAGAGGGACATCTCACGGTTCCTGGAGTGTAATCCAGTGATGATTGATGCCATCAAAGTTTCTGCTGCTCACAGG GCCCGATACTTCTGGGGCAACCTACCCGGGATGAACAGGATCTTTGGCTTTCCTGTGCACTACACAGACGTGTCCAACATGGGCCGTGGTGCCCGCCAGA AGCTGCTGGGAAGGTCCTGGAGCGTGCCTGTCATCCGACACCTCTTCGCCCCTCTGAAGGACTACTTTGCATGTGAATAGTTCCAGCCAGGCCCCAAGCC CACTGGGGTGTGTGGCAGAGCCAGGACCCAGGAGGTGTGATTCCTGAAGGCATCCCCAGGCCCTGCTCTTCCTCAGCTGTGTGGGTCATACCGTGTACCT CAGTTCCCTCTTGCTCAGTGGGGGCAGAGCCACCTGACTCTTGCAGGGGTAGCCTGAGGTGCCGCCTCCTTGTGCACAAATCAGACCTGGCTGCTTGGAG CAGCCTAACACGGTGCTCATTTTTTCTTCTCCTAAAACTTTAAAACTTGAAGTAGGTAGCAACGTGGCTTTTTTTTTTTCCCTTCCTGGGTCTACCACTC AGAGAAACAATGGCTAAGATACCAAAACCACAGTGCCGACAGCTCTCCAATACTCAGGTTAATGCTGAAAAATCATCCAAGACAGTTATTGCAAGAGTTT AATTTTTGAAAACTGGCTACTGCTCTGTGTTTACAGACGTGTGCAGTTGTAGGCATGTAGCTACAGGACATTTTTAAGGGCCCAGGATCGTTTTTTCCCA GGGCAAGCAGAAGAGAAAATGTTGTATATGTCTTTTACCCGGCACATTCCCCTTGCCTAAATACAAGGGCTGGAGTCTGCACGGGACCTATTAGAGTATT TTCCACAATGATGATGATTTCAGCAGGGATGACGTCATCATCACATTCAGGGCTATTTTTTCCCCCACAAACCCAAGGGCAGGGGCCACTCTTAGCTAAA TCCCTCCCCGTGACTGCAATAGAACCCTCTGGGGAGCTCAGGAAGGGGTGTGCTGAGTTCTATAATATAAGCTGCCATATATTTTGTAGACAAGTATGGC TCCTCCATATCTCCCTCTTCCCTAGGAGAGGAGTGTGAAGCAAGGAGCTTAGATAAGACACCCCCTCAAACCCATTCCCTCTCCAGGAGACCTACCCTCC ACAGGCACAGGTCCCCAGATGAGAAGTCTGCTACCCTCATTTCTCATCTTTTTACTAAACTCAGAGGCAGTGACAGCAGTCAGGGACAGACATACATTTC TCATACCTTCCCCACATCTGAGAGATGACAGGGAAAACTGCAAAGCTCGGTGCTCCCTTTGGAGATTTTTTAATCCTTTTTTATTCCATAAGAAGTCGTT TTTAGGGAGAACGGGAATTCAGACAAGCTGCATTTCAGAAATGCTGTCATAATGGTTTTTAACACCTTTTACTCTTCTTACTGGTGCTATTTTGTAGAAT AAGGAACAACGTTGACAAGTTTTGTGGGGCTTTTTATACACTTTTTAAAATCTCAAACTTCTATTTTTATGTTTAACGTTTTCATTAAAATTTTTTTTGT AACTGGAGCCACGACGTAACAAATATGGGGAAAAAACTGTGCCTTGTTTCAACAGTTTTTGCTAATTTTTAGGCTGAAAGATGACGGATGCCTAGAGTTT ACCTTATGTTTAATTAAAATCAGTATTTGTCTATAA

DNMT3B.u.f.26 (forward primer)
DNMT3B.u.r.28 (reverse primer)
Multiple exon skipping

Frame 0

THSRCPVRPAPLPRSSCSRLRGRSPRGRSERPP TDGTGSLAVGRAGGNAARPAALGLSGPSKPSSAI GAGDSRAQRPARPPAGLPPASPDPRLRRPAAPQ PALRQESMKGDTRHLNGEEDAGGREDSILVNGA CSDQSSDSPPILEAIRTPEIRGRRSSSRLSKREV SSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRE TRTRSESPAVRTRNNNSVSSRERHRPSPRSTRG RQGRNHVDESPVEFPATRSLRRRATASAGTPWPS PPSSYLTIDLTDDTEDTHGTPQSSSTPYARLAQ DSQQGGMESPQVEADSGDGDSSEYQDGKEFGIG DLVWGKIKGFSWWPAMVVSWKATSKRQAMSGMRW VQWFGDGKFSEVSADKLVALGLFSQHFNLATFN KLVSYRKAMYHALEKARVRAGKTFPSSPGDSLE DQLKPMLEWAHGGFKPTGIEGLKPNNTQPENKTR RRTADDSATSDYCPAPKRLKTNCYNNGKDRGDE DQSREQMASDVANNKSSLEDGCLSCGRKNPVSF HPLFEGGLCQTCRHLCGDSVKPTHPSCLALCLWG RMGDSCHRGQWHAGHSQLSF*RTPSHQQCVLQL YPRASTSKPAGPALLWSPISLVFLSGCQDRFLE LFYMYDDDGYQSYCTVCCEGRELLLCSNTSCCRC FCVECLEVLVGTGTAAEAKLQEPWSCYMCLPQR CHGVLRRRKDWNVRLQAFFTSDTGLEYEAPKLY PAIPAARRRPIRVLSLFDGIATGYLVLKELGIKV GKYVASEVCEESIAVGTVKHEGNIKYVNDVRNI TKKNIEEWGPFDLVIGGSPCNDLSNVNPARKGL YEGTGRLFFEFYHLLNYSRPKEGDDRPFFWMFEN VVAMKVGDKRDISRFLECNPVMIDAIKVSAAHR ARYFWGNLPGMNRIFGFPVHYTDVSNMGRGARQ KLLGRSWSVPVIRHLFAPLKDYFACE*FQPGPKP TGVCGRARTQEV*FLKASPGPALPQLCGSYRVP QFPLAQWGQSHLTLAGVA*GAASLCTNQTWLLG AA*HGAHFFFS*NFKT*SR*QRGFFFFPSWVYHS EKQWLRYQNHSADSSPILRLMLKNHPRQLLQEF NF*KLATALCLQTCAVVGM*LQDIFKGPGSFFP RASRRENVVYVFYPAHSPCLNTRAGVCTGPIRVF STMMMISAGMTSSSHSGLFFPPQTQGQGPLLAK SLPVTAIEPSGELRKGCAEFYNISCHIFCRQVW LLHISLFPRRGV*SKELR*DTPSNPFPLQETYPP QAQVPR*EVCYPHFSSFY*TQRQ*QQSGTDIHF SYLPHI*EMTGKTAKLGAPFGDFLILFYSIRSR F*GEREFRQAAFQKCCHNGF*HLLLFLLVLFCRI RNNVDKFCGAFYTLFKISNFYFYV*RFH*NFFC NWSHDVTNMGKKLCLVSTVFANF*AER*RMPRV YLMFN*NQYLSI

Frame 1

PTPAAPSGPRRFLAAAAPGSAAAARVDAPSAPR RTGPAPWRSGERAATLPGRQRWG*VAQVNLARR SAPEIREPSALHGRQPASRQPAPTRGSAAQPRPS QPCGRKA*RETPGISMERRTPAGGKTRSSSTGP AATSPPTRPQSWRLSAPRRSEAEDQARDSPRGR CPVC*ATHRT*QAMATGKMGMALTPQSCQSSSGK PGLVQKAQLSELEITTVSPAGRGTGLPHVPPEA GRAATMWTSPPWSSRLPGP*DGGQQHRQERHGR PLPALTLPSTSQTTQRTHMGRPRAAVPPTPA*PR TASRGAWSPRRWRQTVEMETVQSIRMGRSLE*G TSCGERSRASPGGPPWWCLGRPPPSDRLCLACG GSSGLAMASSPRSLQTNWWHWGCSASTLIWPPSI SSSPIEKPCTMLWRKLGCELARPSPAALETHWR TS*SPCWSGPTGASSPLGSRASNPTTRNQRTRL EDAQLTTQPPLTTAPHPSASRQIAITTAKTEGMK IRAENKWLQMLPTTRAAWKMAVCLVAGKTPCPS TLSLRGGSVRHAGTCVETQ*SPLIPAALHSASG AGWGTAVTEANGTQVTASCPFKGHQATSSVFCSC ILGPPPASRQGLPFSGLRFHWCFSLAARIASLS CFTCMMTMAISLTALCAARAESCCFAATRAAAG VSVWSAWRCWWAQAQRPRPSFRSPGAVTCVSRSA VMASCGAGRTGTCACRPSSPVTRGLNMKPPSCT LPFPQPEGGPFESCHCLMASRQAT*SSKSWA*R *ESTSLLKCVRSPLLLEP*STRGISNT*TT*GTS QRKILKNGAHLTW*LAEAHATISQM*IQPGKAC MRVQAGSSSNFTTC*ITHAPRRVMTGRSSGCLR ML*P*RLATRGTSHGSWSVIQ**LMPSKFLLLTG PDTSGATYPG*TGSLAFLCTTQTCPTWAVVPAR SCWEGPGACLSSDTSSPL*RTTLHVNSSSQAPS PLGCVAEPGPRRCDS*RHPQALLFLSCVGHTVYL SSLLLSGGRAT*LLQG*PEVPPPCAQIRPGCLE QPNTVLIFSSPKTLKLEVGSNVAFFFSLPGSTT QRNNG*DTKTTVPTALQYSG*C*KIIQDSYCKSL IFENWLLLCVYRRVQL*ACSYRTFLRAQDRFFP GQAEEKMLYMSFTRHIPLA*IQGLESARDLLEY FPQ***FQQG*RHHHIQGYFFPHKPKGRGHS*LN PSP*LQ*NPLGSSGRGVLSSII*AAIYFVDKYG SSISPSSLGEECEARSLDKTPPQTHSLSRRPTL HRHRSPDEKSATLISHLFTKLRGSDSSQGQTYIS HTFPTSER*QGKLQSSVLPLEIF*SFFIP*EVV FRENGNSDKLHFRNAVIMVFNTFYSSYWCYFVE *GTTLTSFVGLFIHFLKSQTSIFMFNVFIKIFFV TGATT*QIWGKNCALFQQFLLIFRLKDDGCLEF TLCLIKISICL*

Frame 2

PLPLPRPARAASSQQLLPAPRPQPAWTLRAPPD GRDRLPGGRASGRQRCPAGSAGVKWPK*T*LGD RRRRFASPAPCTAASRPPASQPRPAAPPPSRAP ASPAAGKHEGRHQASQWRGGRRREGRLDPRQRGL QRPVLRLAPNPGGYPHPGDQRPKIKLATLQEGG VQSAKLHTGLDRRWRRGRWGWL*HPSHAKALPG NQDSFRKPSCPNSK*QQCLQPGEAQAFPTFHPRP AGPQPCGRVPRGVPGYQVPETAGNSIGRNAMAV PSQLLPYHRPHRRHRGHTWDAPEQQYPLRPPSP GQPAGGHGVPAGGGRQWRWRQFRVSGWEGVWNRG PRVGKDQGLLLVARHGGVLEGHLQATGYVWHAV GPVVWRWQVLRGLCRQTGGTGAVQPAL*FGHLQ *ARLLSKSHVPCSGES*GASWQDLPQQPWRLIGG PAEAHVGVGPRGLQAHWDRGPQTQQHATREQDS KTHS*RLSHL*LLPRTQAPQDKLL*QRQRPRG* RSEPRTNGFRCCQQQEQPGRWLFVLWQEKPRVLP PSL*GGALSDMPAPVWRLSEAHSSQLPCTLPLG QDGGQLSQRPMARRSQPVVLLKDTKPPAVCSAA VS*GLHQQAGRACPSLVSDFTGVSLWLPGSLP*A VLHV**RWLSVLLHCVLRGPRAAALQQHELLPV FLCGVPGGAGGHRHSGRGQASGALELLHVSPAA LSWRPAAPEGLERAPAGLLHQ*HGA*I*SPQAVP CHSRSPKAAHSSPVIV*WHRDRLPSPQRVGHKG RKVRRF*SV*GVHCCWNREARGEYQIRERREEH HKEKY*RMGPI*LGDWRKPMQRSLKCESSQERPV *GYRPALLRILPPAELLTPQGG**PAVLLDV*E CCSHEGWRQEGHLTVPGV*SSDD*CHQSFCCSQ GPILLGQPTRDEQDLWLSCALHRRVQHGPWCPPE AAGKVLERACHPTPLRPSEGLLCM*IVPARPQA HWGVWQSQDPGGVIPEGIPRPCSSSAVWVIPCT SVPSCSVGAEPPDSCRGSLRCRLLVHKSDLAAWS SLTRCSFFLLLKL*NLK*VATWLFFFPFLGLPL RETMAKIPKPQCRQLSNTQVNAEKSSKTVIARV *FLKTGYCSVFTDVCSCRHVATGHF*GPRIVFSQ GKQKRKCCICLLPGTFPLPKYKGWSLHGTY*SI FHNDDDFSRDDVIITFRAIFSPTNPRAGATLS* IPPRDCNRTLWGAQEGVC*VL*YKLPYIL*TSMA PPYLPLP*ERSVKQGA*IRHPLKPIPSPGDLPS TGTGPQMRSLLPSFLIFLLNSEAVTAVRDRHTF LIPSPHLRDDRENCKARCSLWRFFNPFLFHKKSF LGRTGIQTSCISEMLS*WFLTPFTLLTGAIL*N KEQR*QVLWGFLYTF*NLKLLFLCLTFSLKFFL *LEPRRNKYGEKTVPCFNSFC*FLG*KMTDA*SL PYV*LKSVFVY

Sequence DNMT3B.eAug05

GATGGGAAGGAGTTTGGAATAGGGGACCTCGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCCGCCATGGTGGTGTCTTGGAAGGCCACCTCCAAGC GACAGGCTATGTCTGGCATGCGGTGGGTCCAGTGGTTTGGCGATGGCAAGTTCTCCGAGGTCTCTGCAGACAAACTGGTGGCACTGGGGCTGTTCAGCCA GCACTTTAATTTGGCCACCTTCAATAAGCTCGTCTCCTATCGAAAAGCCATGTACCATGCTCTGGAGAAAGCTAGGGTGCGAGCTGGCAAGACCTTCCCC AGCAGCCCTGGAGACTCATTGGAGGACCAGCTGAAGCCCATGTTGGAGTGGGCCCACGGGGGCTTCAAGCCCACTGGGATCGAGGGCCTCAAACCCAACA ACACGCAACCAGTGGTTAATAAGTCGAAGGTGCGTCGTGCAGGCAGTAGGAAATTAGAATCAAGGAAATACGAGAACAAGACTCGAAGACGCACAGCTGA CGACTCAGCCACCTCTGACTACTGCCCCGCACCCAAGCGCCTCAAGACAAATTGCTATAACAACGGCAAAGACCGAGGGGATGAAGATCAGAGCCGAGAA CAAATGGCTTCAGATGTTGCCAACAACAAGAGCAGCCTGGAAGATGGCTGTTTGTCTTGTGGCAGGAAAAACCCCGTGTCCTTCCACCCTCTCTTTGAGG GGGGGCTCTGTCAGACATGCCGGGATCGCTTCCTTGAGCTGTTTTACATGTATGATGACGATGGCTATCAGTCTTACTGCACTGTGTGCTGCGAGGGCCG AGAGCTGCTGCTTTGCAGCAACACGAGCTGCTGCCGGTGTTTCTGTGTGGAGTGCCTGGAGGTGCTGGTGGGCACAGGCACAGCGGCCGAGGCCAAGCTT CAGGAGCCCTGGAGCTGTTACATGTGTCTCCCGCAGCGCTGTCATGGCGTCCTGCGGCGCCGGAAGGACTGGAACGTGCGCCTGCAGGCCTTCTTCACCA GTGACACGGGGCTTGAATATGAAGCCCCCAAGCTGTACCCTGCCATTCCCGCAGCCCGAAGGCGGCCCATTCGAGTCCTGTCATTGTTTGATGGCATCGC GACAGGCTACCTAGTCCTCAAAGAGTTGGGCATAAAGGTAGGAAAGTACGTCGCTTCTGAAGTGTGTGAGGAGTCCATTGCTGTTGGAACCGTGAAGCAC GAGGGGAATATCAAATACGTGAACGACGTGAGGAACATCACAAAGAAAAATATTGAAGAATGGGGCCCATTTGACTTGGTGATTGGCGGAAGCCCATGCA ACGATCTCTCAAATGTGAATCCAGCCAGGAAAGGCCTGTATGAGGGTACAGGCCGGCTCTTCTTCGAATTTTACCACCTGCTGAATTACTCACGCCCCAA GGAGGGTGATGACCGGCCGTTCTTCTGGATGTTTGAGAATGTTGTAGCCATGAAGGTTGGCGACAAGAGGGACATCTCACGGTTCCTGGAGTGTAATCCA GTGATGATTGATGCCATCAAAGTTTCTGCTGCTCACAGGGCCCGATACTTCTGGGGCAACCTACCCGGGATGAACAGGATCTTTGGCTTTCCTGTGCACT ACACAGACGTGTCCAACATGGGCCGTGGTGCCCGCCAGAAGCTGCTGGGAAGGTCCTGGAGCGTGCCTGTCATCCGACACCTCTTCGCCCCTCTGAAGGA CTACTTTGCATGTGAATAGTTCCAGCCAGGCCCCAAGCCCACTGGGGTGTGTGGCAGAGCCAGGACCCAGGAGGTGTGATTCCTGAAGGCATCCCCAGGC CCTGCTCTTCCTCAGCTGTGTGGGTCATACCGTGTACCTCAGTTCCCTCTTGCTCAGTGGGGGCAGAGCCACCTGACTCTTGCAGGGGTAGCCTGAGGTG CCGCCTCCTTGTGCACAAATCAGACCTGGCTGCTTGGAGCAGCCTAACACGGTGCTCATTTTTTCTTCTCCTAAAACTTTAAAACTTGAAGTAGGTAGCA ACGTGGCTTTTTTTTTTTCCCTTCCTGGGTCTACCACTCAGAGAAACAATGGCTAAGATACCAAAACCACAGTGCCGACAGCTCTCCAATACTCAGGTTA ATGCTGAAAAATCATCCAAGACAGTTATTGCAAGAGTTTAATTTTTGAAAACTGGCTACTGCTCTGTGTTTACAGACGTGTGCAGTTGTAGGCATGTAGC TACAGGACATTTTTAAGGGCCCAGGATCGTTTTTTCCCAGGGCAAGCAGAAGAGAAAATGTTGTATATGTCTTTTACCCGGCACATTCCCCTTGCCTAAA TACAAGGGCTGGAGTCTGCACGGGACCTATTAGAGTATTTTCCACAATGATGATGATTTCAGCAGGGATGACGTCATCATCACATTCAGGGCTATTTTTT CCCCCACAAACCCAAGGGCAGGGGCCACTCTTAGCTAAATCCCTCCCCGTGACTGCAATAGAACCCTCTGGGGAGCTCAGGAAGGGGTGTGCTGAGTTCT ATAATATAAGCTGCCATATATTTTGTAGACAAGTATGGCTCCTCCATATCTCCCTCTTCCCTAGGAGAGGAGTGTGAAGCAAGGAGCTTAGATAAGACAC CCCCTCAAACCCATTCCCTCTCCAGGAGACCTACCCTCCACAGGCACAGGTCCCCAGATGAGAAGTCTGCTACCCTCATTTCTCATCTTTTTACTAAACT CAGAGGCAGTGACAGCAGTCAGGGACAGACATACATTTCTCATACCTTCCCCACATCTGAGAGATGACAGGGAAAACTGCAAAGCTCGGTGCTCCCTTTG GAGATTTTTTAATCCTTTTTTATTCCATAAGAAGTCGTTTTTAGGGAGAACGGGAATTCAGACAAGCTGCATTTCAGAAATGCTGTCATAATGGTTTTTA ACACCTTTTACTCTTCTTACTGGTGCTATTTTGTAGAATAAGGAACAACGTTGACAAGTTTTGTGGGGCTTTTTATACACTTTTTAAAATCTCAAACTTC TATTTTTATGTTTAACGTTTTCATTAAAATTTTTTTTGTAACTGGAGCCACGACGTAACAAATATGGGGAAAAAACTGTGCCTTGTTTCAACAGTTTTTG CTAATTTTTAGGCTGAAAGATGACGGATGCCTAGAGTTTACCTTATGTTTAATTAAAATCAGTATTTGTCTATAA

DNMT3B.u.f.26 (forward primer)
DNMT3B.u.r.28 (reverse primer)
Multiple exon skipping

Frame 0

DGKEFGIGDLVWGKIKGFSWWPAMVVSWKATSK RQAMSGMRWVQWFGDGKFSEVSADKLVALGLFSQ HFNLATFNKLVSYRKAMYHALEKARVRAGKTFP SSPGDSLEDQLKPMLEWAHGGFKPTGIEGLKPN NTQPVVNKSKVRRAGSRKLESRKYENKTRRRTAD DSATSDYCPAPKRLKTNCYNNGKDRGDEDQSRE QMASDVANNKSSLEDGCLSCGRKNPVSFHPLFE GGLCQTCRDRFLELFYMYDDDGYQSYCTVCCEGR ELLLCSNTSCCRCFCVECLEVLVGTGTAAEAKL QEPWSCYMCLPQRCHGVLRRRKDWNVRLQAFFT SDTGLEYEAPKLYPAIPAARRRPIRVLSLFDGIA TGYLVLKELGIKVGKYVASEVCEESIAVGTVKH EGNIKYVNDVRNITKKNIEEWGPFDLVIGGSPC NDLSNVNPARKGLYEGTGRLFFEFYHLLNYSRPK EGDDRPFFWMFENVVAMKVGDKRDISRFLECNP VMIDAIKVSAAHRARYFWGNLPGMNRIFGFPVH YTDVSNMGRGARQKLLGRSWSVPVIRHLFAPLKD YFACE*FQPGPKPTGVCGRARTQEV*FLKASPG PALPQLCGSYRVPQFPLAQWGQSHLTLAGVA*G AASLCTNQTWLLGAA*HGAHFFFS*NFKT*SR*Q RGFFFFPSWVYHSEKQWLRYQNHSADSSPILRL MLKNHPRQLLQEFNF*KLATALCLQTCAVVGM* LQDIFKGPGSFFPRASRRENVVYVFYPAHSPCLN TRAGVCTGPIRVFSTMMMISAGMTSSSHSGLFF PPQTQGQGPLLAKSLPVTAIEPSGELRKGCAEF YNISCHIFCRQVWLLHISLFPRRGV*SKELR*DT PSNPFPLQETYPPQAQVPR*EVCYPHFSSFY*T QRQ*QQSGTDIHFSYLPHI*EMTGKTAKLGAPF GDFLILFYSIRSRF*GEREFRQAAFQKCCHNGF* HLLLFLLVLFCRIRNNVDKFCGAFYTLFKISNF YFYV*RFH*NFFCNWSHDVTNMGKKLCLVSTVF ANF*AER*RMPRVYLMFN*NQYLSI

Frame 1

MGRSLE*GTSCGERSRASPGGPPWWCLGRPPPS DRLCLACGGSSGLAMASSPRSLQTNWWHWGCSA STLIWPPSISSSPIEKPCTMLWRKLGCELARPSP AALETHWRTS*SPCWSGPTGASSPLGSRASNPT TRNQWLISRRCVVQAVGN*NQGNTRTRLEDAQL TTQPPLTTAPHPSASRQIAITTAKTEGMKIRAEN KWLQMLPTTRAAWKMAVCLVAGKTPCPSTLSLR GGSVRHAGIASLSCFTCMMTMAISLTALCAARA ESCCFAATRAAAGVSVWSAWRCWWAQAQRPRPSF RSPGAVTCVSRSAVMASCGAGRTGTCACRPSSP VTRGLNMKPPSCTLPFPQPEGGPFESCHCLMAS RQAT*SSKSWA*R*ESTSLLKCVRSPLLLEP*ST RGISNT*TT*GTSQRKILKNGAHLTW*LAEAHA TISQM*IQPGKACMRVQAGSSSNFTTC*ITHAP RRVMTGRSSGCLRML*P*RLATRGTSHGSWSVIQ **LMPSKFLLLTGPDTSGATYPG*TGSLAFLCT TQTCPTWAVVPARSCWEGPGACLSSDTSSPL*R TTLHVNSSSQAPSPLGCVAEPGPRRCDS*RHPQA LLFLSCVGHTVYLSSLLLSGGRAT*LLQG*PEV PPPCAQIRPGCLEQPNTVLIFSSPKTLKLEVGS NVAFFFSLPGSTTQRNNG*DTKTTVPTALQYSG* C*KIIQDSYCKSLIFENWLLLCVYRRVQL*ACS YRTFLRAQDRFFPGQAEEKMLYMSFTRHIPLA* IQGLESARDLLEYFPQ***FQQG*RHHHIQGYFF PHKPKGRGHS*LNPSP*LQ*NPLGSSGRGVLSS II*AAIYFVDKYGSSISPSSLGEECEARSLDKT PPQTHSLSRRPTLHRHRSPDEKSATLISHLFTKL RGSDSSQGQTYISHTFPTSER*QGKLQSSVLPL EIF*SFFIP*EVVFRENGNSDKLHFRNAVIMVF NTFYSSYWCYFVE*GTTLTSFVGLFIHFLKSQTS IFMFNVFIKIFFVTGATT*QIWGKNCALFQQFL LIFRLKDDGCLEFTLCLIKISICL*

Frame 2

WEGVWNRGPRVGKDQGLLLVARHGGVLEGHLQA TGYVWHAVGPVVWRWQVLRGLCRQTGGTGAVQP AL*FGHLQ*ARLLSKSHVPCSGES*GASWQDLP QQPWRLIGGPAEAHVGVGPRGLQAHWDRGPQTQQ HATSG**VEGASCRQ*EIRIKEIREQDSKTHS* RLSHL*LLPRTQAPQDKLL*QRQRPRG*RSEPR TNGFRCCQQQEQPGRWLFVLWQEKPRVLPPSL*G GALSDMPGSLP*AVLHV**RWLSVLLHCVLRGP RAAALQQHELLPVFLCGVPGGAGGHRHSGRGQA SGALELLHVSPAALSWRPAAPEGLERAPAGLLHQ *HGA*I*SPQAVPCHSRSPKAAHSSPVIV*WHR DRLPSPQRVGHKGRKVRRF*SV*GVHCCWNREA RGEYQIRERREEHHKEKY*RMGPI*LGDWRKPMQ RSLKCESSQERPV*GYRPALLRILPPAELLTPQ GG**PAVLLDV*ECCSHEGWRQEGHLTVPGV*S SDD*CHQSFCCSQGPILLGQPTRDEQDLWLSCAL HRRVQHGPWCPPEAAGKVLERACHPTPLRPSEG LLCM*IVPARPQAHWGVWQSQDPGGVIPEGIPR PCSSSAVWVIPCTSVPSCSVGAEPPDSCRGSLRC RLLVHKSDLAAWSSLTRCSFFLLLKL*NLK*VA TWLFFFPFLGLPLRETMAKIPKPQCRQLSNTQV NAEKSSKTVIARV*FLKTGYCSVFTDVCSCRHVA TGHF*GPRIVFSQGKQKRKCCICLLPGTFPLPK YKGWSLHGTY*SIFHNDDDFSRDDVIITFRAIF SPTNPRAGATLS*IPPRDCNRTLWGAQEGVC*VL *YKLPYIL*TSMAPPYLPLP*ERSVKQGA*IRH PLKPIPSPGDLPSTGTGPQMRSLLPSFLIFLLN SEAVTAVRDRHTFLIPSPHLRDDRENCKARCSLW RFFNPFLFHKKSFLGRTGIQTSCISEMLS*WFL TPFTLLTGAIL*NKEQR*QVLWGFLYTF*NLKL LFLCLTFSLKFFL*LEPRRNKYGEKTVPCFNSFC *FLG*KMTDA*SLPYV*LKSVFVY

Sequence DNMT3B.cAug05

ACCCACTCCCGCTGCCCCGTCCGGCCCGCGCCGCTTCCTCGCAGCAGCTGCTCCCGGCTCCGCGGCCGCAGCCCGCGTGGACGCTCCGAGCGCCCCCCGA CGGACGGGACCGGCTCCCTGGCGGTCGGGCGAGCGGGCGGCAACGCTGCCCGGCCGGCAGCGCTGGGGTTAAGTGGCCCAAGTAAACCTAGCTCGGCGAT CGGCGCCGGAGATTCGCGAGCCCAGCGCCCTGCACGGCCGCCAGCCGGCCTCCCGCCAGCCAGCCCCGACCCGCGGCTCCGCCGCCCAGCCGCGCCCCAG CCAGCCCTGCGGCAGGAAAGCATGAAGGGAGACACCAGGCATCTCAATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCGATCCTCGTCAACGGGGCCT GCAGCGACCAGTCCTCCGACTCGCCCCCAATCCTGGAGGCTATCCGCACCCCGGAGATCAGAGGCCGAAGATCAAGCTCGCGACTCTCCAAGAGGGAGGT GTCCAGTCTGCTAAGCTACACACAGGACTTGACAGGCGATGGCGACGGGGAAGATGGGGATGGCTCTGACACCCCAGTCATGCCAAAGCTCTTCCGGGAA ACCAGGACTCGTTCAGAAAGCCCAGCTGTCCGAACTCGAAATAACAACAGTGTCTCCAGCCGGGAGAGGCACAGGCCTTCCCCACGTTCCACCCGAGGCC GGCAGGGCCGCAACCATGTGGACGAGTCCCCCGTGGAGTTCCCGGCTACCAGGTCCCTGAGACGGCGGGCAACAGCATCGGCAGGAACGCCATGGCCGTC CCCTCCCAGCTCTTACCTTACCATCGACCTCACAGACGACACAGAGGACACACATGGGACGCCCCAGAGCAGCAGTACCCCCTACGCCCGCCTAGCCCAG GACAGCCAGCAGGGGGGCATGGAGTCCCCGCAGGTGGAGGCAGACAGTGGAGATGGAGACAGTTCAGAGTATCAGGATGGGAAGGAGTTTGGAATAGGGG ACCTCGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCCGCCATGGTGGTGTCTTGGAAGGCCACCTCCAAGCGACAGGCTATGTCTGGCATGCGGTG GGTCCAGTGGTTTGGCGATGGCAAGTTCTCCGAGGTCTCTGCAGACAAACTGGTGGCACTGGGGCTGTTCAGCCAGCACTTTAATTTGGCCACCTTCAAT AAGCTCGTCTCCTATCGAAAAGCCATGTACCATGCTCTGGAGAAAGCTAGGGTGCGAGCTGGCAAGACCTTCCCCAGCAGCCCTGGAGACTCATTGGAGG ACCAGCTGAAGCCCATGTTGGAGTGGGCCCACGGGGGCTTCAAGCCCACTGGGATCGAGGGCCTCAAACCCAACAACACGCAACCAGAGAACAAGACTCG AAGACGCACAGCTGACGACTCAGCCACCTCTGACTACTGCCCCGCACCCAAGCGCCTCAAGACAAATTGCTATAACAACGGCAAAGACCGAGGGGATGAA GATCAGAGCCGAGAACAAATGGCTTCAGATGTTGCCAACAACAAGAGCAGCCTGGAAGATGGCTGTTTGTCTTGTGGCAGGAAAAACCCCGTGTCCTTCC ACCCTCTCTTTGAGGGGGGGCTCTGTCAGACATGCCGGGATCGCTTCCTTGAGCTGTTTTACATGTATGATGACGATGGCTATCAGTCTTACTGCACTGT GTGCTGCGAGGGCCGAGAGCTGCTGCTTTGCAGCAACACGAGCTGCTGCCGGTGTTTCTGTGTGGAGTGCCTGGAGGTGCTGGTGGGCACAGGCACAGCG GCCGAGGCCAAGCTTCAGGAGCCCTGGAGCTGTTACATGTGTCTCCCGCAGCGCTGTCATGGCGTCCTGCGGCGCCGGAAGGACTGGAACGTGCGCCTGC AGGCCTTCTTCACCAGTGACACGGGGCTTGAATATGAAGCCCCCAAGCTGTACCCTGCCATTCCCGCAGCCCGAAGGCGGCCCATTCGAGTCCTGTCATT GTTTGATGGCATCGCGACAGGCTACCTAGTCCTCAAAGAGTTGGGCATAAAGGTAGGAAAGTACGTCGCTTCTGAAGTGTGTGAGGAGTCCATTGCTGTT GGAACCGTGAAGCACGAGGGGAATATCAAATACGTGAACGACGTGAGGAACATCACAAAGAAAAATATTGAAGAATGGGGCCCATTTGACTTGGTGATTG GCGGAAGCCCATGCAACGATCTCTCAAATGTGAATCCAGCCAGGAAAGGCCTGTATGAGGGTACAGGCCGGCTCTTCTTCGAATTTTACCACCTGCTGAA TTACTCACGCCCCAAGGAGGGTGATGACCGGCCGTTCTTCTGGATGTTTGAGAATGTTGTAGCCATGAAGGTTGGCGACAAGAGGGACATCTCACGGTTC CTGGAGTGTAATCCAGTGATGATTGATGCCATCAAAGTTTCTGCTGCTCACAGGGCCCGATACTTCTGGGGCAACCTACCCGGGATGAACAGGATCTTTG GCTTTCCTGTGCACTACACAGACGTGTCCAACATGGGCCGTGGTGCCCGCCAGAAGCTGCTGGGAAGGTCCTGGAGCGTGCCTGTCATCCGACACCTCTT CGCCCCTCTGAAGGACTACTTTGCATGTGAATAGTTCCAGCCAGGCCCCAAGCCCACTGGGGTGTGTGGCAGAGCCAGGACCCAGGAGGTGTGATTCCTG AAGGCATCCCCAGGCCCTGCTCTTCCTCAGCTGTGTGGGTCATACCGTGTACCTCAGTTCCCTCTTGCTCAGTGGGGGCAGAGCCACCTGACTCTTGCAG GGGTAGCCTGAGGTGCCGCCTCCTTGTGCACAAATCAGACCTGGCTGCTTGGAGCAGCCTAACACGGTGCTCATTTTTTCTTCTCCTAAAACTTTAAAAC TTGAAGTAGGTAGCAACGTGGCTTTTTTTTTTTCCCTTCCTGGGTCTACCACTCAGAGAAACAATGGCTAAGATACCAAAACCACAGTGCCGACAGCTCT CCAATACTCAGGTTAATGCTGAAAAATCATCCAAGACAGTTATTGCAAGAGTTTAATTTTTGAAAACTGGCTACTGCTCTGTGTTTACAGACGTGTGCAG TTGTAGGCATGTAGCTACAGGACATTTTTAAGGGCCCAGGATCGTTTTTTCCCAGGGCAAGCAGAAGAGAAAATGTTGTATATGTCTTTTACCCGGCACA TTCCCCTTGCCTAAATACAAGGGCTGGAGTCTGCACGGGACCTATTAGAGTATTTTCCACAATGATGATGATTTCAGCAGGGATGACGTCATCATCACAT TCAGGGCTATTTTTTCCCCCACAAACCCAAGGGCAGGGGCCACTCTTAGCTAAATCCCTCCCCGTGACTGCAATAGAACCCTCTGGGGAGCTCAGGAAGG GGTGTGCTGAGTTCTATAATATAAGCTGCCATATATTTTGTAGACAAGTATGGCTCCTCCATATCTCCCTCTTCCCTAGGAGAGGAGTGTGAAGCAAGGA GCTTAGATAAGACACCCCCTCAAACCCATTCCCTCTCCAGGAGACCTACCCTCCACAGGCACAGGTCCCCAGATGAGAAGTCTGCTACCCTCATTTCTCA TCTTTTTACTAAACTCAGAGGCAGTGACAGCAGTCAGGGACAGACATACATTTCTCATACCTTCCCCACATCTGAGAGATGACAGGGAAAACTGCAAAGC TCGGTGCTCCCTTTGGAGATTTTTTAATCCTTTTTTATTCCATAAGAAGTCGTTTTTAGGGAGAACGGGAATTCAGACAAGCTGCATTTCAGAAATGCTG TCATAATGGTTTTTAACACCTTTTACTCTTCTTACTGGTGCTATTTTGTAGAATAAGGAACAACGTTGACAAGTTTTGTGGGGCTTTTTATACACTTTTT AAAATCTCAAACTTCTATTTTTATGTTTAACGTTTTCATTAAAATTTTTTTTGTAACTGGAGCCACGACGTAACAAATATGGGGAAAAAACTGTGCCTTG TTTCAACAGTTTTTGCTAATTTTTAGGCTGAAAGATGACGGATGCCTAGAGTTTACCTTATGTTTAATTAAAATCAGTATTTGTCTATAA

DNMT3B.u.f.26 (forward primer)
DNMT3B.u.r.28 (reverse primer)
Multiple exon skipping

Frame 0

THSRCPVRPAPLPRSSCSRLRGRSPRGRSERPP TDGTGSLAVGRAGGNAARPAALGLSGPSKPSSAI GAGDSRAQRPARPPAGLPPASPDPRLRRPAAPQ PALRQESMKGDTRHLNGEEDAGGREDSILVNGA CSDQSSDSPPILEAIRTPEIRGRRSSSRLSKREV SSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRE TRTRSESPAVRTRNNNSVSSRERHRPSPRSTRG RQGRNHVDESPVEFPATRSLRRRATASAGTPWPS PPSSYLTIDLTDDTEDTHGTPQSSSTPYARLAQ DSQQGGMESPQVEADSGDGDSSEYQDGKEFGIG DLVWGKIKGFSWWPAMVVSWKATSKRQAMSGMRW VQWFGDGKFSEVSADKLVALGLFSQHFNLATFN KLVSYRKAMYHALEKARVRAGKTFPSSPGDSLE DQLKPMLEWAHGGFKPTGIEGLKPNNTQPENKTR RRTADDSATSDYCPAPKRLKTNCYNNGKDRGDE DQSREQMASDVANNKSSLEDGCLSCGRKNPVSF HPLFEGGLCQTCRDRFLELFYMYDDDGYQSYCTV CCEGRELLLCSNTSCCRCFCVECLEVLVGTGTA AEAKLQEPWSCYMCLPQRCHGVLRRRKDWNVRL QAFFTSDTGLEYEAPKLYPAIPAARRRPIRVLSL FDGIATGYLVLKELGIKVGKYVASEVCEESIAV GTVKHEGNIKYVNDVRNITKKNIEEWGPFDLVI GGSPCNDLSNVNPARKGLYEGTGRLFFEFYHLLN YSRPKEGDDRPFFWMFENVVAMKVGDKRDISRF LECNPVMIDAIKVSAAHRARYFWGNLPGMNRIF GFPVHYTDVSNMGRGARQKLLGRSWSVPVIRHLF APLKDYFACE*FQPGPKPTGVCGRARTQEV*FL KASPGPALPQLCGSYRVPQFPLAQWGQSHLTLA GVA*GAASLCTNQTWLLGAA*HGAHFFFS*NFKT *SR*QRGFFFFPSWVYHSEKQWLRYQNHSADSS PILRLMLKNHPRQLLQEFNF*KLATALCLQTCA VVGM*LQDIFKGPGSFFPRASRRENVVYVFYPAH SPCLNTRAGVCTGPIRVFSTMMMISAGMTSSSH SGLFFPPQTQGQGPLLAKSLPVTAIEPSGELRK GCAEFYNISCHIFCRQVWLLHISLFPRRGV*SKE LR*DTPSNPFPLQETYPPQAQVPR*EVCYPHFS SFY*TQRQ*QQSGTDIHFSYLPHI*EMTGKTAK LGAPFGDFLILFYSIRSRF*GEREFRQAAFQKCC HNGF*HLLLFLLVLFCRIRNNVDKFCGAFYTLF KISNFYFYV*RFH*NFFCNWSHDVTNMGKKLCL VSTVFANF*AER*RMPRVYLMFN*NQYLSI

Frame 1

PTPAAPSGPRRFLAAAAPGSAAAARVDAPSAPR RTGPAPWRSGERAATLPGRQRWG*VAQVNLARR SAPEIREPSALHGRQPASRQPAPTRGSAAQPRPS QPCGRKA*RETPGISMERRTPAGGKTRSSSTGP AATSPPTRPQSWRLSAPRRSEAEDQARDSPRGR CPVC*ATHRT*QAMATGKMGMALTPQSCQSSSGK PGLVQKAQLSELEITTVSPAGRGTGLPHVPPEA GRAATMWTSPPWSSRLPGP*DGGQQHRQERHGR PLPALTLPSTSQTTQRTHMGRPRAAVPPTPA*PR TASRGAWSPRRWRQTVEMETVQSIRMGRSLE*G TSCGERSRASPGGPPWWCLGRPPPSDRLCLACG GSSGLAMASSPRSLQTNWWHWGCSASTLIWPPSI SSSPIEKPCTMLWRKLGCELARPSPAALETHWR TS*SPCWSGPTGASSPLGSRASNPTTRNQRTRL EDAQLTTQPPLTTAPHPSASRQIAITTAKTEGMK IRAENKWLQMLPTTRAAWKMAVCLVAGKTPCPS TLSLRGGSVRHAGIASLSCFTCMMTMAISLTAL CAARAESCCFAATRAAAGVSVWSAWRCWWAQAQR PRPSFRSPGAVTCVSRSAVMASCGAGRTGTCAC RPSSPVTRGLNMKPPSCTLPFPQPEGGPFESCH CLMASRQAT*SSKSWA*R*ESTSLLKCVRSPLLL EP*STRGISNT*TT*GTSQRKILKNGAHLTW*L AEAHATISQM*IQPGKACMRVQAGSSSNFTTC* ITHAPRRVMTGRSSGCLRML*P*RLATRGTSHGS WSVIQ**LMPSKFLLLTGPDTSGATYPG*TGSL AFLCTTQTCPTWAVVPARSCWEGPGACLSSDTS SPL*RTTLHVNSSSQAPSPLGCVAEPGPRRCDS* RHPQALLFLSCVGHTVYLSSLLLSGGRAT*LLQ G*PEVPPPCAQIRPGCLEQPNTVLIFSSPKTLK LEVGSNVAFFFSLPGSTTQRNNG*DTKTTVPTAL QYSG*C*KIIQDSYCKSLIFENWLLLCVYRRVQ L*ACSYRTFLRAQDRFFPGQAEEKMLYMSFTRH IPLA*IQGLESARDLLEYFPQ***FQQG*RHHHI QGYFFPHKPKGRGHS*LNPSP*LQ*NPLGSSGR GVLSSII*AAIYFVDKYGSSISPSSLGEECEAR SLDKTPPQTHSLSRRPTLHRHRSPDEKSATLISH LFTKLRGSDSSQGQTYISHTFPTSER*QGKLQS SVLPLEIF*SFFIP*EVVFRENGNSDKLHFRNA VIMVFNTFYSSYWCYFVE*GTTLTSFVGLFIHFL KSQTSIFMFNVFIKIFFVTGATT*QIWGKNCAL FQQFLLIFRLKDDGCLEFTLCLIKISICL*

Frame 2

PLPLPRPARAASSQQLLPAPRPQPAWTLRAPPD GRDRLPGGRASGRQRCPAGSAGVKWPK*T*LGD RRRRFASPAPCTAASRPPASQPRPAAPPPSRAP ASPAAGKHEGRHQASQWRGGRRREGRLDPRQRGL QRPVLRLAPNPGGYPHPGDQRPKIKLATLQEGG VQSAKLHTGLDRRWRRGRWGWL*HPSHAKALPG NQDSFRKPSCPNSK*QQCLQPGEAQAFPTFHPRP AGPQPCGRVPRGVPGYQVPETAGNSIGRNAMAV PSQLLPYHRPHRRHRGHTWDAPEQQYPLRPPSP GQPAGGHGVPAGGGRQWRWRQFRVSGWEGVWNRG PRVGKDQGLLLVARHGGVLEGHLQATGYVWHAV GPVVWRWQVLRGLCRQTGGTGAVQPAL*FGHLQ *ARLLSKSHVPCSGES*GASWQDLPQQPWRLIGG PAEAHVGVGPRGLQAHWDRGPQTQQHATREQDS KTHS*RLSHL*LLPRTQAPQDKLL*QRQRPRG* RSEPRTNGFRCCQQQEQPGRWLFVLWQEKPRVLP PSL*GGALSDMPGSLP*AVLHV**RWLSVLLHC VLRGPRAAALQQHELLPVFLCGVPGGAGGHRHS GRGQASGALELLHVSPAALSWRPAAPEGLERAPA GLLHQ*HGA*I*SPQAVPCHSRSPKAAHSSPVI V*WHRDRLPSPQRVGHKGRKVRRF*SV*GVHCC WNREARGEYQIRERREEHHKEKY*RMGPI*LGDW RKPMQRSLKCESSQERPV*GYRPALLRILPPAE LLTPQGG**PAVLLDV*ECCSHEGWRQEGHLTV PGV*SSDD*CHQSFCCSQGPILLGQPTRDEQDLW LSCALHRRVQHGPWCPPEAAGKVLERACHPTPL RPSEGLLCM*IVPARPQAHWGVWQSQDPGGVIP EGIPRPCSSSAVWVIPCTSVPSCSVGAEPPDSCR GSLRCRLLVHKSDLAAWSSLTRCSFFLLLKL*N LK*VATWLFFFPFLGLPLRETMAKIPKPQCRQL SNTQVNAEKSSKTVIARV*FLKTGYCSVFTDVCS CRHVATGHF*GPRIVFSQGKQKRKCCICLLPGT FPLPKYKGWSLHGTY*SIFHNDDDFSRDDVIIT FRAIFSPTNPRAGATLS*IPPRDCNRTLWGAQEG VC*VL*YKLPYIL*TSMAPPYLPLP*ERSVKQG A*IRHPLKPIPSPGDLPSTGTGPQMRSLLPSFL IFLLNSEAVTAVRDRHTFLIPSPHLRDDRENCKA RCSLWRFFNPFLFHKKSFLGRTGIQTSCISEML S*WFLTPFTLLTGAIL*NKEQR*QVLWGFLYTF *NLKLLFLCLTFSLKFFL*LEPRRNKYGEKTVPC FNSFC*FLG*KMTDA*SLPYV*LKSVFVY

Sequence DNMT3B.dAug05

GAAGGGAGAGAGCAAACAAAGGGGTCAGGAGGGAAAATAATGCACTGGCTTCCTGAGCCCCTGCAGAGGCTGAGCAGGGAGAGGGGGCCAGGGCCAGAGG GGACAGAGGCTGGCGGCAGACGGGCCGGGACAGGCAGGTCCTAAATGGCATTGTTTGAAGGGGCCGGCTAATTGCACAGAGCAGTCTGAGCCTGAGACCC CAGCCCTGGCCTCCCCACTCTGTCCTGGGTGCTGGCGTCTGAGCCTTCGGGACAGCCTGTCCACATGGAACCAAGTCCTGAGCCTCCAAGCTTGGAAAGC ATGAAGGGAGACACCAGGCATCTCAATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCGATCCTCGTCAACGGGGCCTGCAGCGACCAGTCCTCCGACT CGCCCCCAATCCTGGAGGCTATCCGCACCCCGGAGATCAGAGGCCGAAGATCAAGCTCGCGACTCTCCAAGAGGGAGGTGTCCAGTCTGCTAAGCTACAC ACAGGACTTGACAGGCGATGGCGACGGGGAAGATGGGGATGGCTCTGACACCCCAGTCATGCCAAAGCTCTTCCGGGAAACCAGGACTCGTTCAGAAAGC CCAGCTGTCCGAACTCGAAATAACAACAGTGTCTCCAGCCGGGAGAGGCACAGGCCTTCCCCACGTTCCACCCGAGGCCGGCAGGGCCGCAACCATGTGG ACGAGTCCCCCGTGGAGTTCCCGGCTACCAGGTCCCTGAGACGGCGGGCAACAGCATCGGCAGGAACGCCATGGCCGTCCCCTCCCAGCTCTTACCTTAC CATCGACCTCACAGACGACACAGAGGACACACATGGGACGCCCCAGAGCAGCAGTACCCCCTACGCCCGCCTAGCCCAGGACAGCCAGCAGGGGGGCATG GAGTCCCCGCAGGTGGAGGCAGACAGTGGAGATGGAGACAGTTCAGAGTATCAGGATGGGAAGGAGTTTGGAATAGGGGACCTCGTGTGGGGAAAGATCA AGGGCTTCTCCTGGTGGCCCGCCATGGTGGTGTCTTGGAAGGCCACCTCCAAGCGACAGGCTATGTCTGGCATGCGGTGGGTCCAGTGGTTTGGCGATGG CAAGTTCTCCGAGGTCTCTGCAGACAAACTGGTGGCACTGGGGCTGTTCAGCCAGCACTTTAATTTGGCCACCTTCAATAAGCTCGTCTCCTATCGAAAA GCCATGTACCATGCTCTGGAGAAAGCTAGGGTGCGAGCTGGCAAGACCTTCCCCAGCAGCCCTGGAGACTCATTGGAGGACCAGCTGAAGCCCATGTTGG AGTGGGCCCACGGGGGCTTCAAGCCCACTGGGATCGAGGGCCTCAAACCCAACAACACGCAACCAGAGAACAAGACTCGAAGACGCACAGCTGACGACTC AGCCACCTCTGACTACTGCCCCGCACCCAAGCGCCTCAAGACAAATTGCTATAACAACGGCAAAGACCGAGGGGATGAAGATCAGAGCCGAGAACAAATG GCTTCAGATGTTGCCAACAACAAGAGCAGCCTGGAAGATGGCTGTTTGTCTTGTGGCAGGAAAAACCCCGTGTCCTTCCACCCTCTCTTTGAGGGGGGGC TCTGTCAGACATGCCGGGATCGCTTCCTTGAGCTGTTTTACATGTATGATGACGATGGCTATCAGTCTTACTGCACTGTGTGCTGCGAGGGCCGAGAGCT GCTGCTTTGCAGCAACACGAGCTGCTGCCGGTGTTTCTGTGTGGAGTGCCTGGAGGTGCTGGTGGGCACAGGCACAGCGGCCGAGGCCAAGCTTCAGGAG CCCTGGAGCTGTTACATGTGTCTCCCGCAGCGCTGTCATGGCGTCCTGCGGCGCCGGAAGGACTGGAACGTGCGCCTGCAGGCCTTCTTCACCAGTGACA CGGGGCTTGAATATGAAGCCCCCAAGCTGTACCCTGCCATTCCCGCAGCCCGAAGGCGGCCCATTCGAGTCCTGTCATTGTTTGATGGCATCGCGACAGG CTACCTAGTCCTCAAAGAGTTGGGCATAAAGGTAGGAAAGTACGTCGCTTCTGAAGTGTGTGAGGAGTCCATTGCTGTTGGAACCGTGAAGCACGAGGGG AATATCAAATACGTGAACGACGTGAGGAACATCACAAAGAAAAATATTGAAGAATGGGGCCCATTTGACTTGGTGATTGGCGGAAGCCCATGCAACGATC TCTCAAATGTGAATCCAGCCAGGAAAGGCCTGTATGAGGGTACAGGCCGGCTCTTCTTCGAATTTTACCACCTGCTGAATTACTCACGCCCCAAGGAGGG TGATGACCGGCCGTTCTTCTGGATGTTTGAGAATGTTGTAGCCATGAAGGTTGGCGACAAGAGGGACATCTCACGGTTCCTGGAGTGTAATCCAGTGATG ATTGATGCCATCAAAGTTTCTGCTGCTCACAGGGCCCGATACTTCTGGGGCAACCTACCCGGGATGAACAGGCCCGTGATAGCATCAAAGAATGATAAAC TCGAGCTGCAGGACTGCTTGGAATACAATAGGATAGCCAAGTTAAAGAAAGTACAGACAATAACCACCAAGTCGAACTCGATCAAACAGGGGAAAAACCA ACTTTTCCCTGTTGTCATGAATGGCAAAGAAGATGTTTTGTGGTGCACTGAGCTCGAAAGGATCTTTGGCTTTCCTGTGCACTACACAGACGTGTCCAAC ATGGGCCGTGGTGCCCGCCAGAAGCTGCTGGGAAGGTCCTGGAGCGTGCCTGTCATCCGACACCTCTTCGCCCCTCTGAAGGACTACTTTGCATGTGAAT AGTTCCAGCCAGGCCCCAAGCCCACTGGGGTGTGTGGCAGAGCCAGGACCCAGGAGGTGTGATTCCTGAAGGCATCCCCAGGCCCTGCTCTTCCTCAGCT GTGTGGGTCATACCGTGTACCTCAGTTCCCTCTTGCTCAGTGGGGGCAGAGCCACCTGACTCTTGCAGGGGTAGCCTGAGGTGCCGCCTCCTTGTGCACA AATCAGACCTGGCTGCTTGGAGCAGCCTAACACGGTGCTCATTTTTTCTTCTCCTAAAACTTTAAAACTTGAAGTAGGTAGCAACGTGGCTTTTTTTTTT TCCCTTCCTGGGTCTACCACTCAGAGAAACAATGGCTAAGATACCAAAACCACAGTGCCGACAGCTCTCCAATACTCAGGTTAATGCTGAAAAATCATCC AAGACAGTTATTGCAAGAGTTTAATTTTTGAAAACTGGCTACTGCTCTGTGTTTACAGACGTGTGCAGTTGTAGGCATGTAGCTACAGGACATTTTTAAG GGCCCAGGATCGTTTTTTCCCAGGGCAAGCAGAAGAGAAAATGTTGTATATGTCTTTTACCCGGCACATTCCCCTTGCCTAAATACAAGGGCTGGAGTCT GCACGGGACCTATTAGAGTATTTTCCACAATGATGATGATTTCAGCAGGGATGACGTCATCATCACATTCAGGGCTATTTTTTCCCCCACAAACCCAAGG GCAGGGGCCACTCTTAGCTAAATCCCTCCCCGTGACTGCAATAGAACCCTCTGGGGAGCTCAGGAAGGGGTGTGCTGAGTTCTATAATATAAGCTGCCAT ATATTTTGTAGACAAGTATGGCTCCTCCATATCTCCCTCTTCCCTAGGAGAGGAGTGTGAAGCAAGGAGCTTAGATAAGACACCCCCTCAAACCCATTCC CTCTCCAGGAGACCTACCCTCCACAGGCACAGGTCCCCAGATGAGAAGTCTGCTACCCTCATTTCTCATCTTTTTACTAAACTCAGAGGCAGTGACAGCA GTCAGGGACAGACATACATTTCTCATACCTTCCCCACATCTGAGAGATGACAGGGAAAACTGCAAAGCTCGGTGCTCCCTTTGGAGATTTTTTAATCCTT TTTTATTCCATAAGAAGTCGTTTTTAGGGAGAACGGGAATTCAGACAAGCTGCATTTCAGAAATGCTGTCATAATGGTTTTTAACACCTTTTACTCTTCT TACTGGTGCTATTTTGTAGAATAAGGAACAACGTTGACAAGTTTTGTGGGGCTTTTTATACACTTTTTAAAATCTCAAACTTCTATTTTTATGTTTAACG TTTTCATTAAAATTTTTTTTGTAACTGGAGCCACGACGTAACAAATATGGGGAAAAAACTGTGCCTTGTTTCAACAGTTTTTGCTAATTTTTAGGCTGAA AGATGACGGATGCCTAGAGTTTACCTTATGTTTAATTAAAATCAGTATTTGTCTATAA

DNMT3B.u.f.26 (forward primer)
DNMT3B.u.r.28 (reverse primer)
Multiple exon skipping

Frame 0

EGREQTKGSGGKIMHWLPEPLQRLSRERGPGPE GTEAGGRRAGTGRS*MALFEGAG*LHRAV*A*DP SPGLPTLSWVLASEPSGQPVHMEPSPEPPSLES MKGDTRHLNGEEDAGGREDSILVNGACSDQSSD SPPILEAIRTPEIRGRRSSSRLSKREVSSLLSYT QDLTGDGDGEDGDGSDTPVMPKLFRETRTRSES PAVRTRNNNSVSSRERHRPSPRSTRGRQGRNHV DESPVEFPATRSLRRRATASAGTPWPSPPSSYLT IDLTDDTEDTHGTPQSSSTPYARLAQDSQQGGM ESPQVEADSGDGDSSEYQDGKEFGIGDLVWGKI KGFSWWPAMVVSWKATSKRQAMSGMRWVQWFGDG KFSEVSADKLVALGLFSQHFNLATFNKLVSYRK AMYHALEKARVRAGKTFPSSPGDSLEDQLKPML EWAHGGFKPTGIEGLKPNNTQPENKTRRRTADDS ATSDYCPAPKRLKTNCYNNGKDRGDEDQSREQM ASDVANNKSSLEDGCLSCGRKNPVSFHPLFEGG LCQTCRDRFLELFYMYDDDGYQSYCTVCCEGREL LLCSNTSCCRCFCVECLEVLVGTGTAAEAKLQE PWSCYMCLPQRCHGVLRRRKDWNVRLQAFFTSD TGLEYEAPKLYPAIPAARRRPIRVLSLFDGIATG YLVLKELGIKVGKYVASEVCEESIAVGTVKHEG NIKYVNDVRNITKKNIEEWGPFDLVIGGSPCND LSNVNPARKGLYEGTGRLFFEFYHLLNYSRPKEG DDRPFFWMFENVVAMKVGDKRDISRFLECNPVM IDAIKVSAAHRARYFWGNLPGMNRPVIASKNDK LELQDCLEYNRIAKLKKVQTITTKSNSIKQGKNQ LFPVVMNGKEDVLWCTELERIFGFPVHYTDVSN MGRGARQKLLGRSWSVPVIRHLFAPLKDYFACE *FQPGPKPTGVCGRARTQEV*FLKASPGPALPQL CGSYRVPQFPLAQWGQSHLTLAGVA*GAASLCT NQTWLLGAA*HGAHFFFS*NFKT*SR*QRGFFF FPSWVYHSEKQWLRYQNHSADSSPILRLMLKNHP RQLLQEFNF*KLATALCLQTCAVVGM*LQDIFK GPGSFFPRASRRENVVYVFYPAHSPCLNTRAGV CTGPIRVFSTMMMISAGMTSSSHSGLFFPPQTQG QGPLLAKSLPVTAIEPSGELRKGCAEFYNISCH IFCRQVWLLHISLFPRRGV*SKELR*DTPSNPF PLQETYPPQAQVPR*EVCYPHFSSFY*TQRQ*QQ SGTDIHFSYLPHI*EMTGKTAKLGAPFGDFLIL FYSIRSRF*GEREFRQAAFQKCCHNGF*HLLLF LLVLFCRIRNNVDKFCGAFYTLFKISNFYFYV*R FH*NFFCNWSHDVTNMGKKLCLVSTVFANF*AE R*RMPRVYLMFN*NQYLSI

Frame 1

KGESKQRGQEGK*CTGFLSPCRG*AGRGGQGQR GQRLAADGPGQAGPKWHCLKGPANCTEQSEPET PALASPLCPGCWRLSLRDSLSTWNQVLSLQAWKA *RETPGISMERRTPAGGKTRSSSTGPAATSPPT RPQSWRLSAPRRSEAEDQARDSPRGRCPVC*AT HRT*QAMATGKMGMALTPQSCQSSSGKPGLVQKA QLSELEITTVSPAGRGTGLPHVPPEAGRAATMW TSPPWSSRLPGP*DGGQQHRQERHGRPLPALTL PSTSQTTQRTHMGRPRAAVPPTPA*PRTASRGAW SPRRWRQTVEMETVQSIRMGRSLE*GTSCGERS RASPGGPPWWCLGRPPPSDRLCLACGGSSGLAM ASSPRSLQTNWWHWGCSASTLIWPPSISSSPIEK PCTMLWRKLGCELARPSPAALETHWRTS*SPCW SGPTGASSPLGSRASNPTTRNQRTRLEDAQLTT QPPLTTAPHPSASRQIAITTAKTEGMKIRAENKW LQMLPTTRAAWKMAVCLVAGKTPCPSTLSLRGG SVRHAGIASLSCFTCMMTMAISLTALCAARAES CCFAATRAAAGVSVWSAWRCWWAQAQRPRPSFRS PGAVTCVSRSAVMASCGAGRTGTCACRPSSPVT RGLNMKPPSCTLPFPQPEGGPFESCHCLMASRQ AT*SSKSWA*R*ESTSLLKCVRSPLLLEP*STRG ISNT*TT*GTSQRKILKNGAHLTW*LAEAHATI SQM*IQPGKACMRVQAGSSSNFTTC*ITHAPRR VMTGRSSGCLRML*P*RLATRGTSHGSWSVIQ** LMPSKFLLLTGPDTSGATYPG*TGP**HQRMIN SSCRTAWNTIG*PS*RKYRQ*PPSRTRSNRGKT NFSLLS*MAKKMFCGALSSKGSLAFLCTTQTCPT WAVVPARSCWEGPGACLSSDTSSPL*RTTLHVN SSSQAPSPLGCVAEPGPRRCDS*RHPQALLFLS CVGHTVYLSSLLLSGGRAT*LLQG*PEVPPPCAQ IRPGCLEQPNTVLIFSSPKTLKLEVGSNVAFFF SLPGSTTQRNNG*DTKTTVPTALQYSG*C*KII QDSYCKSLIFENWLLLCVYRRVQL*ACSYRTFLR AQDRFFPGQAEEKMLYMSFTRHIPLA*IQGLES ARDLLEYFPQ***FQQG*RHHHIQGYFFPHKPK GRGHS*LNPSP*LQ*NPLGSSGRGVLSSII*AAI YFVDKYGSSISPSSLGEECEARSLDKTPPQTHS LSRRPTLHRHRSPDEKSATLISHLFTKLRGSDS SQGQTYISHTFPTSER*QGKLQSSVLPLEIF*SF FIP*EVVFRENGNSDKLHFRNAVIMVFNTFYSS YWCYFVE*GTTLTSFVGLFIHFLKSQTSIFMFN VFIKIFFVTGATT*QIWGKNCALFQQFLLIFRLK DDGCLEFTLCLIKISICL*

Frame 2

RERANKGVRRENNALAS*APAEAEQGEGARARG DRGWRQTGRDRQVLNGIV*RGRLIAQSSLSLRP QPWPPHSVLGAGV*AFGTACPHGTKS*ASKLGK HEGRHQASQWRGGRRREGRLDPRQRGLQRPVLRL APNPGGYPHPGDQRPKIKLATLQEGGVQSAKLH TGLDRRWRRGRWGWL*HPSHAKALPGNQDSFRK PSCPNSK*QQCLQPGEAQAFPTFHPRPAGPQPCG RVPRGVPGYQVPETAGNSIGRNAMAVPSQLLPY HRPHRRHRGHTWDAPEQQYPLRPPSPGQPAGGH GVPAGGGRQWRWRQFRVSGWEGVWNRGPRVGKDQ GLLLVARHGGVLEGHLQATGYVWHAVGPVVWRW QVLRGLCRQTGGTGAVQPAL*FGHLQ*ARLLSK SHVPCSGES*GASWQDLPQQPWRLIGGPAEAHVG VGPRGLQAHWDRGPQTQQHATREQDSKTHS*RL SHL*LLPRTQAPQDKLL*QRQRPRG*RSEPRTN GFRCCQQQEQPGRWLFVLWQEKPRVLPPSL*GGA LSDMPGSLP*AVLHV**RWLSVLLHCVLRGPRA AALQQHELLPVFLCGVPGGAGGHRHSGRGQASG ALELLHVSPAALSWRPAAPEGLERAPAGLLHQ*H GA*I*SPQAVPCHSRSPKAAHSSPVIV*WHRDR LPSPQRVGHKGRKVRRF*SV*GVHCCWNREARG EYQIRERREEHHKEKY*RMGPI*LGDWRKPMQRS LKCESSQERPV*GYRPALLRILPPAELLTPQGG **PAVLLDV*ECCSHEGWRQEGHLTVPGV*SSD D*CHQSFCCSQGPILLGQPTRDEQARDSIKE**T RAAGLLGIQ*DSQVKESTDNNHQVELDQTGEKP TFPCCHEWQRRCFVVH*ARKDLWLSCALHRRVQ HGPWCPPEAAGKVLERACHPTPLRPSEGLLCM*I VPARPQAHWGVWQSQDPGGVIPEGIPRPCSSSA VWVIPCTSVPSCSVGAEPPDSCRGSLRCRLLVH KSDLAAWSSLTRCSFFLLLKL*NLK*VATWLFFF PFLGLPLRETMAKIPKPQCRQLSNTQVNAEKSS KTVIARV*FLKTGYCSVFTDVCSCRHVATGHF* GPRIVFSQGKQKRKCCICLLPGTFPLPKYKGWSL HGTY*SIFHNDDDFSRDDVIITFRAIFSPTNPR AGATLS*IPPRDCNRTLWGAQEGVC*VL*YKLP YIL*TSMAPPYLPLP*ERSVKQGA*IRHPLKPIP SPGDLPSTGTGPQMRSLLPSFLIFLLNSEAVTA VRDRHTFLIPSPHLRDDRENCKARCSLWRFFNP FLFHKKSFLGRTGIQTSCISEMLS*WFLTPFTLL TGAIL*NKEQR*QVLWGFLYTF*NLKLLFLCLT FSLKFFL*LEPRRNKYGEKTVPCFNSFC*FLG* KMTDA*SLPYV*LKSVFVY

Sequence DNMT3B.bAug05

ACCCACTCCCGCTGCCCCGTCCGGCCCGCGCCGCTTCCTCGCAGCAGCTGCTCCCGGCTCCGCGGCCGCAGCCCGCGTGGACGCTCCGAGCGCCCCCCGA CGGACGGGACCGGCTCCCTGGCGGTCGGGCGAGCGGGCGGCAACGCTGCCCGGCCGGCAGCGCTGGGGTTAAGTGGCCCAAGTAAACCTAGCTCGGCGAT CGGCGCCGGAGATTCGCGAGCCCAGCGCCCTGCACGGCCGCCAGCCGGCCTCCCGCCAGCCAGCCCCGACCCGCGGCTCCGCCGCCCAGCCGCGCCCCAG CCAGCCCTGCGGCAGGAAAGCATGAAGGGAGACACCAGGCATCTCAATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCGATCCTCGTCAACGGGGCCT GCAGCGACCAGTCCTCCGACTCGCCCCCAATCCTGGAGGCTATCCGCACCCCGGAGATCAGAGGCCGAAGATCAAGCTCGCGACTCTCCAAGAGGGAGGT GTCCAGTCTGCTAAGCTACACACAGGACTTGACAGGCGATGGCGACGGGGAAGATGGGGATGGCTCTGACACCCCAGTCATGCCAAAGCTCTTCCGGGAA ACCAGGACTCGTTCAGAAAGCCCAGCTGTCCGAACTCGAAATAACAACAGTGTCTCCAGCCGGGAGAGGCACAGGCCTTCCCCACGTTCCACCCGAGGCC GGCAGGGCCGCAACCATGTGGACGAGTCCCCCGTGGAGTTCCCGGCTACCAGGTCCCTGAGACGGCGGGCAACAGCATCGGCAGGAACGCCATGGCCGTC CCCTCCCAGCTCTTACCTTACCATCGACCTCACAGACGACACAGAGGACACACATGGGACGCCCCAGAGCAGCAGTACCCCCTACGCCCGCCTAGCCCAG GACAGCCAGCAGGGGGGCATGGAGTCCCCGCAGGTGGAGGCAGACAGTGGAGATGGAGACAGTTCAGAGTATCAGGATGGGAAGGAGTTTGGAATAGGGG ACCTCGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCCGCCATGGTGGTGTCTTGGAAGGCCACCTCCAAGCGACAGGCTATGTCTGGCATGCGGTG GGTCCAGTGGTTTGGCGATGGCAAGTTCTCCGAGGTCTCTGCAGACAAACTGGTGGCACTGGGGCTGTTCAGCCAGCACTTTAATTTGGCCACCTTCAAT AAGCTCGTCTCCTATCGAAAAGCCATGTACCATGCTCTGGAGAAAGCTAGGGTGCGAGCTGGCAAGACCTTCCCCAGCAGCCCTGGAGACTCATTGGAGG ACCAGCTGAAGCCCATGTTGGAGTGGGCCCACGGGGGCTTCAAGCCCACTGGGATCGAGGGCCTCAAACCCAACAACACGCAACCAGAGAACAAGACTCG AAGACGCACAGCTGACGACTCAGCCACCTCTGACTACTGCCCCGCACCCAAGCGCCTCAAGACAAATTGCTATAACAACGGCAAAGACCGAGGGGATGAA GATCAGAGCCGAGAACAAATGGCTTCAGATGTTGCCAACAACAAGAGCAGCCTGGAAGATGGCTGTTTGTCTTGTGGCAGGAAAAACCCCGTGTCCTTCC ACCCTCTCTTTGAGGGGGGGCTCTGTCAGACATGCCGGGATCGCTTCCTTGAGCTGTTTTACATGTATGATGACGATGGCTATCAGTCTTACTGCACTGT GTGCTGCGAGGGCCGAGAGCTGCTGCTTTGCAGCAACACGAGCTGCTGCCGGTGTTTCTGTGTGGAGTGCCTGGAGGTGCTGGTGGGCACAGGCACAGCG GCCGAGGCCAAGCTTCAGGAGCCCTGGAGCTGTTACATGTGTCTCCCGCAGCGCTGTCATGGCGTCCTGCGGCGCCGGAAGGACTGGAACGTGCGCCTGC AGGCCTTCTTCACCAGTGACACGGGGCTTGAATATGAAGCCCCCAAGCTGTACCCTGCCATTCCCGCAGCCCGAAGGCGGCCCATTCGAGTCCTGTCATT GTTTGATGGCATCGCGACAGGCTACCTAGTCCTCAAAGAGTTGGGCATAAAGGTAGGAAAGTACGTCGCTTCTGAAGTGTGTGAGGAGTCCATTGCTGTT GGAACCGTGAAGCACGAGGGGAATATCAAATACGTGAACGACGTGAGGAACATCACAAAGAAAAATATTGAAGAATGGGGCCCATTTGACTTGGTGATTG GCGGAAGCCCATGCAACGATCTCTCAAATGTGAATCCAGCCAGGAAAGGCCTGTATGAGGGTACAGGCCGGCTCTTCTTCGAATTTTACCACCTGCTGAA TTACTCACGCCCCAAGGAGGGTGATGACCGGCCGTTCTTCTGGATGTTTGAGAATGTTGTAGCCATGAAGGTTGGCGACAAGAGGGACATCTCACGGTTC CTGGAGTGTAATCCAGTGATGATTGATGCCATCAAAGTTTCTGCTGCTCACAGGGCCCGATACTTCTGGGGCAACCTACCCGGGATGAACAGGCCCGTGA TAGCATCAAAGAATGATAAACTCGAGCTGCAGGACTGCTTGGAATACAATAGGATAGCCAAGTTAAAGAAAGTACAGACAATAACCACCAAGTCGAACTC GATCAAACAGGGGAAAAACCAACTTTTCCCTGTTGTCATGAATGGCAAAGAAGATGTTTTGTGGTGCACTGAGCTCGAAAGGATCTTTGGCTTTCCTGTG CACTACACAGACGTGTCCAACATGGGCCGTGGTGCCCGCCAGAAGCTGCTGGGAAGGTCCTGGAGCGTGCCTGTCATCCGACACCTCTTCGCCCCTCTGA AGGACTACTTTGCATGTGAATAGTTCCAGCCAGGCCCCAAGCCCACTGGGGTGTGTGGCAGAGCCAGGACCCAGGAGGTGTGATTCCTGAAGGCATCCCC AGGCCCTGCTCTTCCTCAGCTGTGTGGGTCATACCGTGTACCTCAGTTCCCTCTTGCTCAGTGGGGGCAGAGCCACCTGACTCTTGCAGGGGTAGCCTGA GGTGCCGCCTCCTTGTGCACAAATCAGACCTGGCTGCTTGGAGCAGCCTAACACGGTGCTCATTTTTTCTTCTCCTAAAACTTTAAAACTTGAAGTAGGT AGCAACGTGGCTTTTTTTTTTTCCCTTCCTGGGTCTACCACTCAGAGAAACAATGGCTAAGATACCAAAACCACAGTGCCGACAGCTCTCCAATACTCAG GTTAATGCTGAAAAATCATCCAAGACAGTTATTGCAAGAGTTTAATTTTTGAAAACTGGCTACTGCTCTGTGTTTACAGACGTGTGCAGTTGTAGGCATG TAGCTACAGGACATTTTTAAGGGCCCAGGATCGTTTTTTCCCAGGGCAAGCAGAAGAGAAAATGTTGTATATGTCTTTTACCCGGCACATTCCCCTTGCC TAAATACAAGGGCTGGAGTCTGCACGGGACCTATTAGAGTATTTTCCACAATGATGATGATTTCAGCAGGGATGACGTCATCATCACATTCAGGGCTATT TTTTCCCCCACAAACCCAAGGGCAGGGGCCACTCTTAGCTAAATCCCTCCCCGTGACTGCAATAGAACCCTCTGGGGAGCTCAGGAAGGGGTGTGCTGAG TTCTATAATATAAGCTGCCATATATTTTGTAGACAAGTATGGCTCCTCCATATCTCCCTCTTCCCTAGGAGAGGAGTGTGAAGCAAGGAGCTTAGATAAG ACACCCCCTCAAACCCATTCCCTCTCCAGGAGACCTACCCTCCACAGGCACAGGTCCCCAGATGAGAAGTCTGCTACCCTCATTTCTCATCTTTTTACTA AACTCAGAGGCAGTGACAGCAGTCAGGGACAGACATACATTTCTCATACCTTCCCCACATCTGAGAGATGACAGGGAAAACTGCAAAGCTCGGTGCTCCC TTTGGAGATTTTTTAATCCTTTTTTATTCCATAAGAAGTCGTTTTTAGGGAGAACGGGAATTCAGACAAGCTGCATTTCAGAAATGCTGTCATAATGGTT TTTAACACCTTTTACTCTTCTTACTGGTGCTATTTTGTAGAATAAGGAACAACGTTGACAAGTTTTGTGGGGCTTTTTATACACTTTTTAAAATCTCAAA CTTCTATTTTTATGTTTAACGTTTTCATTAAAATTTTTTTTGTAACTGGAGCCACGACGTAACAAATATGGGGAAAAAACTGTGCCTTGTTTCAACAGTT TTTGCTAATTTTTAGGCTGAAAGATGACGGATGCCTAGAGTTTACCTTATGTTTAATTAAAATCAGTATTTGTCTATAA

DNMT3B.u.f.26 (forward primer)
DNMT3B.u.r.28 (reverse primer)
Multiple exon skipping

Frame 0

THSRCPVRPAPLPRSSCSRLRGRSPRGRSERPP TDGTGSLAVGRAGGNAARPAALGLSGPSKPSSAI GAGDSRAQRPARPPAGLPPASPDPRLRRPAAPQ PALRQESMKGDTRHLNGEEDAGGREDSILVNGA CSDQSSDSPPILEAIRTPEIRGRRSSSRLSKREV SSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRE TRTRSESPAVRTRNNNSVSSRERHRPSPRSTRG RQGRNHVDESPVEFPATRSLRRRATASAGTPWPS PPSSYLTIDLTDDTEDTHGTPQSSSTPYARLAQ DSQQGGMESPQVEADSGDGDSSEYQDGKEFGIG DLVWGKIKGFSWWPAMVVSWKATSKRQAMSGMRW VQWFGDGKFSEVSADKLVALGLFSQHFNLATFN KLVSYRKAMYHALEKARVRAGKTFPSSPGDSLE DQLKPMLEWAHGGFKPTGIEGLKPNNTQPENKTR RRTADDSATSDYCPAPKRLKTNCYNNGKDRGDE DQSREQMASDVANNKSSLEDGCLSCGRKNPVSF HPLFEGGLCQTCRDRFLELFYMYDDDGYQSYCTV CCEGRELLLCSNTSCCRCFCVECLEVLVGTGTA AEAKLQEPWSCYMCLPQRCHGVLRRRKDWNVRL QAFFTSDTGLEYEAPKLYPAIPAARRRPIRVLSL FDGIATGYLVLKELGIKVGKYVASEVCEESIAV GTVKHEGNIKYVNDVRNITKKNIEEWGPFDLVI GGSPCNDLSNVNPARKGLYEGTGRLFFEFYHLLN YSRPKEGDDRPFFWMFENVVAMKVGDKRDISRF LECNPVMIDAIKVSAAHRARYFWGNLPGMNRPV IASKNDKLELQDCLEYNRIAKLKKVQTITTKSNS IKQGKNQLFPVVMNGKEDVLWCTELERIFGFPV HYTDVSNMGRGARQKLLGRSWSVPVIRHLFAPL KDYFACE*FQPGPKPTGVCGRARTQEV*FLKASP GPALPQLCGSYRVPQFPLAQWGQSHLTLAGVA* GAASLCTNQTWLLGAA*HGAHFFFS*NFKT*SR *QRGFFFFPSWVYHSEKQWLRYQNHSADSSPILR LMLKNHPRQLLQEFNF*KLATALCLQTCAVVGM *LQDIFKGPGSFFPRASRRENVVYVFYPAHSPC LNTRAGVCTGPIRVFSTMMMISAGMTSSSHSGLF FPPQTQGQGPLLAKSLPVTAIEPSGELRKGCAE FYNISCHIFCRQVWLLHISLFPRRGV*SKELR* DTPSNPFPLQETYPPQAQVPR*EVCYPHFSSFY* TQRQ*QQSGTDIHFSYLPHI*EMTGKTAKLGAP FGDFLILFYSIRSRF*GEREFRQAAFQKCCHNG F*HLLLFLLVLFCRIRNNVDKFCGAFYTLFKISN FYFYV*RFH*NFFCNWSHDVTNMGKKLCLVSTV FANF*AER*RMPRVYLMFN*NQYLSI

Frame 1

PTPAAPSGPRRFLAAAAPGSAAAARVDAPSAPR RTGPAPWRSGERAATLPGRQRWG*VAQVNLARR SAPEIREPSALHGRQPASRQPAPTRGSAAQPRPS QPCGRKA*RETPGISMERRTPAGGKTRSSSTGP AATSPPTRPQSWRLSAPRRSEAEDQARDSPRGR CPVC*ATHRT*QAMATGKMGMALTPQSCQSSSGK PGLVQKAQLSELEITTVSPAGRGTGLPHVPPEA GRAATMWTSPPWSSRLPGP*DGGQQHRQERHGR PLPALTLPSTSQTTQRTHMGRPRAAVPPTPA*PR TASRGAWSPRRWRQTVEMETVQSIRMGRSLE*G TSCGERSRASPGGPPWWCLGRPPPSDRLCLACG GSSGLAMASSPRSLQTNWWHWGCSASTLIWPPSI SSSPIEKPCTMLWRKLGCELARPSPAALETHWR TS*SPCWSGPTGASSPLGSRASNPTTRNQRTRL EDAQLTTQPPLTTAPHPSASRQIAITTAKTEGMK IRAENKWLQMLPTTRAAWKMAVCLVAGKTPCPS TLSLRGGSVRHAGIASLSCFTCMMTMAISLTAL CAARAESCCFAATRAAAGVSVWSAWRCWWAQAQR PRPSFRSPGAVTCVSRSAVMASCGAGRTGTCAC RPSSPVTRGLNMKPPSCTLPFPQPEGGPFESCH CLMASRQAT*SSKSWA*R*ESTSLLKCVRSPLLL EP*STRGISNT*TT*GTSQRKILKNGAHLTW*L AEAHATISQM*IQPGKACMRVQAGSSSNFTTC* ITHAPRRVMTGRSSGCLRML*P*RLATRGTSHGS WSVIQ**LMPSKFLLLTGPDTSGATYPG*TGP* *HQRMINSSCRTAWNTIG*PS*RKYRQ*PPSRT RSNRGKTNFSLLS*MAKKMFCGALSSKGSLAFLC TTQTCPTWAVVPARSCWEGPGACLSSDTSSPL* RTTLHVNSSSQAPSPLGCVAEPGPRRCDS*RHP QALLFLSCVGHTVYLSSLLLSGGRAT*LLQG*PE VPPPCAQIRPGCLEQPNTVLIFSSPKTLKLEVG SNVAFFFSLPGSTTQRNNG*DTKTTVPTALQYS G*C*KIIQDSYCKSLIFENWLLLCVYRRVQL*AC SYRTFLRAQDRFFPGQAEEKMLYMSFTRHIPLA *IQGLESARDLLEYFPQ***FQQG*RHHHIQGY FFPHKPKGRGHS*LNPSP*LQ*NPLGSSGRGVLS SII*AAIYFVDKYGSSISPSSLGEECEARSLDK TPPQTHSLSRRPTLHRHRSPDEKSATLISHLFT KLRGSDSSQGQTYISHTFPTSER*QGKLQSSVLP LEIF*SFFIP*EVVFRENGNSDKLHFRNAVIMV FNTFYSSYWCYFVE*GTTLTSFVGLFIHFLKSQ TSIFMFNVFIKIFFVTGATT*QIWGKNCALFQQF LLIFRLKDDGCLEFTLCLIKISICL*

Frame 2

PLPLPRPARAASSQQLLPAPRPQPAWTLRAPPD GRDRLPGGRASGRQRCPAGSAGVKWPK*T*LGD RRRRFASPAPCTAASRPPASQPRPAAPPPSRAP ASPAAGKHEGRHQASQWRGGRRREGRLDPRQRGL QRPVLRLAPNPGGYPHPGDQRPKIKLATLQEGG VQSAKLHTGLDRRWRRGRWGWL*HPSHAKALPG NQDSFRKPSCPNSK*QQCLQPGEAQAFPTFHPRP AGPQPCGRVPRGVPGYQVPETAGNSIGRNAMAV PSQLLPYHRPHRRHRGHTWDAPEQQYPLRPPSP GQPAGGHGVPAGGGRQWRWRQFRVSGWEGVWNRG PRVGKDQGLLLVARHGGVLEGHLQATGYVWHAV GPVVWRWQVLRGLCRQTGGTGAVQPAL*FGHLQ *ARLLSKSHVPCSGES*GASWQDLPQQPWRLIGG PAEAHVGVGPRGLQAHWDRGPQTQQHATREQDS KTHS*RLSHL*LLPRTQAPQDKLL*QRQRPRG* RSEPRTNGFRCCQQQEQPGRWLFVLWQEKPRVLP PSL*GGALSDMPGSLP*AVLHV**RWLSVLLHC VLRGPRAAALQQHELLPVFLCGVPGGAGGHRHS GRGQASGALELLHVSPAALSWRPAAPEGLERAPA GLLHQ*HGA*I*SPQAVPCHSRSPKAAHSSPVI V*WHRDRLPSPQRVGHKGRKVRRF*SV*GVHCC WNREARGEYQIRERREEHHKEKY*RMGPI*LGDW RKPMQRSLKCESSQERPV*GYRPALLRILPPAE LLTPQGG**PAVLLDV*ECCSHEGWRQEGHLTV PGV*SSDD*CHQSFCCSQGPILLGQPTRDEQARD SIKE**TRAAGLLGIQ*DSQVKESTDNNHQVEL DQTGEKPTFPCCHEWQRRCFVVH*ARKDLWLSC ALHRRVQHGPWCPPEAAGKVLERACHPTPLRPSE GLLCM*IVPARPQAHWGVWQSQDPGGVIPEGIP RPCSSSAVWVIPCTSVPSCSVGAEPPDSCRGSL RCRLLVHKSDLAAWSSLTRCSFFLLLKL*NLK*V ATWLFFFPFLGLPLRETMAKIPKPQCRQLSNTQ VNAEKSSKTVIARV*FLKTGYCSVFTDVCSCRH VATGHF*GPRIVFSQGKQKRKCCICLLPGTFPLP KYKGWSLHGTY*SIFHNDDDFSRDDVIITFRAI FSPTNPRAGATLS*IPPRDCNRTLWGAQEGVC* VL*YKLPYIL*TSMAPPYLPLP*ERSVKQGA*IR HPLKPIPSPGDLPSTGTGPQMRSLLPSFLIFLL NSEAVTAVRDRHTFLIPSPHLRDDRENCKARCS LWRFFNPFLFHKKSFLGRTGIQTSCISEMLS*WF LTPFTLLTGAIL*NKEQR*QVLWGFLYTF*NLK LLFLCLTFSLKFFL*LEPRRNKYGEKTVPCFNS FC*FLG*KMTDA*SLPYV*LKSVFVY

Sequence DNMT3B.aAug05

ACCCACTCCCGCTGCCCCGTCCGGCCCGCGCCGCTTCCTCGCAGCAGCTGCTCCCGGCTCCGCGGCCGCAGCCCGCGTGGACGCTCCGAGCGCCCCCCGA CGGACGGGACCGGCTCCCTGGCGGTCGGGCGAGCGGGCGGCAACGCTGCCCGGCCGGCAGCGCTGGGGTTAAGTGGCCCAAGTAAACCTAGCTCGGCGAT CGGCGCCGGAGATTCGCGAGCCCAGCGCCCTGCACGGCCGCCAGCCGGCCTCCCGCCAGCCAGCCCCGACCCGCGGCTCCGCCGCCCAGCCGCGCCCCAG CCAGCCCTGCGGCAGGAAAGCATGAAGGGAGACACCAGGCATCTCAATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCGATCCTCGTCAACGGGGCCT GCAGCGACCAGTCCTCCGACTCGCCCCCAATCCTGGAGGCTATCCGCACCCCGGAGATCAGAGGCCGAAGATCAAGCTCGCGACTCTCCAAGAGGGAGGT GTCCAGTCTGCTAAGCTACACACAGGACTTGACAGGCGATGGCGACGGGGAAGATGGGGATGGCTCTGACACCCCAGTCATGCCAAAGCTCTTCCGGGAA ACCAGGACTCGTTCAGAAAGCCCAGCTGTCCGAACTCGAAATAACAACAGTGTCTCCAGCCGGGAGAGGCACAGGCCTTCCCCACGTTCCACCCGAGGCC GGCAGGGCCGCAACCATGTGGACGAGTCCCCCGTGGAGTTCCCGGCTACCAGGTCCCTGAGACGGCGGGCAACAGCATCGGCAGGAACGCCATGGCCGTC CCCTCCCAGCTCTTACCTTACCATCGACCTCACAGACGACACAGAGGACACACATGGGACGCCCCAGAGCAGCAGTACCCCCTACGCCCGCCTAGCCCAG GACAGCCAGCAGGGGGGCATGGAGTCCCCGCAGGTGGAGGCAGACAGTGGAGATGGAGACAGTTCAGAGTATCAGGATGGGAAGGAGTTTGGAATAGGGG ACCTCGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCCGCCATGGTGGTGTCTTGGAAGGCCACCTCCAAGCGACAGGCTATGTCTGGCATGCGGTG GGTCCAGTGGTTTGGCGATGGCAAGTTCTCCGAGGTCTCTGCAGACAAACTGGTGGCACTGGGGCTGTTCAGCCAGCACTTTAATTTGGCCACCTTCAAT AAGCTCGTCTCCTATCGAAAAGCCATGTACCATGCTCTGGAGAAAGCTAGGGTGCGAGCTGGCAAGACCTTCCCCAGCAGCCCTGGAGACTCATTGGAGG ACCAGCTGAAGCCCATGTTGGAGTGGGCCCACGGGGGCTTCAAGCCCACTGGGATCGAGGGCCTCAAACCCAACAACACGCAACCAGTGGTTAATAAGTC GAAGGTGCGTCGTGCAGGCAGTAGGAAATTAGAATCAAGGAAATACGAGAACAAGACTCGAAGACGCACAGCTGACGACTCAGCCACCTCTGACTACTGC CCCGCACCCAAGCGCCTCAAGACAAATTGCTATAACAACGGCAAAGACCGAGGGGATGAAGATCAGAGCCGAGAACAAATGGCTTCAGATGTTGCCAACA ACAAGAGCAGCCTGGAAGATGGCTGTTTGTCTTGTGGCAGGAAAAACCCCGTGTCCTTCCACCCTCTCTTTGAGGGGGGGCTCTGTCAGACATGCCGGGA TCGCTTCCTTGAGCTGTTTTACATGTATGATGACGATGGCTATCAGTCTTACTGCACTGTGTGCTGCGAGGGCCGAGAGCTGCTGCTTTGCAGCAACACG AGCTGCTGCCGGTGTTTCTGTGTGGAGTGCCTGGAGGTGCTGGTGGGCACAGGCACAGCGGCCGAGGCCAAGCTTCAGGAGCCCTGGAGCTGTTACATGT GTCTCCCGCAGCGCTGTCATGGCGTCCTGCGGCGCCGGAAGGACTGGAACGTGCGCCTGCAGGCCTTCTTCACCAGTGACACGGGGCTTGAATATGAAGC CCCCAAGCTGTACCCTGCCATTCCCGCAGCCCGAAGGCGGCCCATTCGAGTCCTGTCATTGTTTGATGGCATCGCGACAGGCTACCTAGTCCTCAAAGAG TTGGGCATAAAGGTAGGAAAGTACGTCGCTTCTGAAGTGTGTGAGGAGTCCATTGCTGTTGGAACCGTGAAGCACGAGGGGAATATCAAATACGTGAACG ACGTGAGGAACATCACAAAGAAAAATATTGAAGAATGGGGCCCATTTGACTTGGTGATTGGCGGAAGCCCATGCAACGATCTCTCAAATGTGAATCCAGC CAGGAAAGGCCTGTATGAGGGTACAGGCCGGCTCTTCTTCGAATTTTACCACCTGCTGAATTACTCACGCCCCAAGGAGGGTGATGACCGGCCGTTCTTC TGGATGTTTGAGAATGTTGTAGCCATGAAGGTTGGCGACAAGAGGGACATCTCACGGTTCCTGGAGTGTAATCCAGTGATGATTGATGCCATCAAAGTTT CTGCTGCTCACAGGGCCCGATACTTCTGGGGCAACCTACCCGGGATGAACAGGCCCGTGATAGCATCAAAGAATGATAAACTCGAGCTGCAGGACTGCTT GGAATACAATAGGATAGCCAAGTTAAAGAAAGTACAGACAATAACCACCAAGTCGAACTCGATCAAACAGGGGAAAAACCAACTTTTCCCTGTTGTCATG AATGGCAAAGAAGATGTTTTGTGGTGCACTGAGCTCGAAAGGATCTTTGGCTTTCCTGTGCACTACACAGACGTGTCCAACATGGGCCGTGGTGCCCGCC AGAAGCTGCTGGGAAGGTCCTGGAGCGTGCCTGTCATCCGACACCTCTTCGCCCCTCTGAAGGACTACTTTGCATGTGAATAGTTCCAGCCAGGCCCCAA GCCCACTGGGGTGTGTGGCAGAGCCAGGACCCAGGAGGTGTGATTCCTGAAGGCATCCCCAGGCCCTGCTCTTCCTCAGCTGTGTGGGTCATACCGTGTA CCTCAGTTCCCTCTTGCTCAGTGGGGGCAGAGCCACCTGACTCTTGCAGGGGTAGCCTGAGGTGCCGCCTCCTTGTGCACAAATCAGACCTGGCTGCTTG GAGCAGCCTAACACGGTGCTCATTTTTTCTTCTCCTAAAACTTTAAAACTTGAAGTAGGTAGCAACGTGGCTTTTTTTTTTTCCCTTCCTGGGTCTACCA CTCAGAGAAACAATGGCTAAGATACCAAAACCACAGTGCCGACAGCTCTCCAATACTCAGGTTAATGCTGAAAAATCATCCAAGACAGTTATTGCAAGAG TTTAATTTTTGAAAACTGGCTACTGCTCTGTGTTTACAGACGTGTGCAGTTGTAGGCATGTAGCTACAGGACATTTTTAAGGGCCCAGGATCGTTTTTTC CCAGGGCAAGCAGAAGAGAAAATGTTGTATATGTCTTTTACCCGGCACATTCCCCTTGCCTAAATACAAGGGCTGGAGTCTGCACGGGACCTATTAGAGT ATTTTCCACAATGATGATGATTTCAGCAGGGATGACGTCATCATCACATTCAGGGCTATTTTTTCCCCCACAAACCCAAGGGCAGGGGCCACTCTTAGCT AAATCCCTCCCCGTGACTGCAATAGAACCCTCTGGGGAGCTCAGGAAGGGGTGTGCTGAGTTCTATAATATAAGCTGCCATATATTTTGTAGACAAGTAT GGCTCCTCCATATCTCCCTCTTCCCTAGGAGAGGAGTGTGAAGCAAGGAGCTTAGATAAGACACCCCCTCAAACCCATTCCCTCTCCAGGAGACCTACCC TCCACAGGCACAGGTCCCCAGATGAGAAGTCTGCTACCCTCATTTCTCATCTTTTTACTAAACTCAGAGGCAGTGACAGCAGTCAGGGACAGACATACAT TTCTCATACCTTCCCCACATCTGAGAGATGACAGGGAAAACTGCAAAGCTCGGTGCTCCCTTTGGAGATTTTTTAATCCTTTTTTATTCCATAAGAAGTC GTTTTTAGGGAGAACGGGAATTCAGACAAGCTGCATTTCAGAAATGCTGTCATAATGGTTTTTAACACCTTTTACTCTTCTTACTGGTGCTATTTTGTAG AATAAGGAACAACGTTGACAAGTTTTGTGGGGCTTTTTATACACTTTTTAAAATCTCAAACTTCTATTTTTATGTTTAACGTTTTCATTAAAATTTTTTT TGTAACTGGAGCCACGACGTAACAAATATGGGGAAAAAACTGTGCCTTGTTTCAACAGTTTTTGCTAATTTTTAGGCTGAAAGATGACGGATGCCTAGAG TTTACCTTATGTTTAATTAAAATCAGTATTTGTCTATAA

DNMT3B.u.f.26 (forward primer)
DNMT3B.u.r.28 (reverse primer)
Multiple exon skipping

Frame 0

THSRCPVRPAPLPRSSCSRLRGRSPRGRSERPP TDGTGSLAVGRAGGNAARPAALGLSGPSKPSSAI GAGDSRAQRPARPPAGLPPASPDPRLRRPAAPQ PALRQESMKGDTRHLNGEEDAGGREDSILVNGA CSDQSSDSPPILEAIRTPEIRGRRSSSRLSKREV SSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRE TRTRSESPAVRTRNNNSVSSRERHRPSPRSTRG RQGRNHVDESPVEFPATRSLRRRATASAGTPWPS PPSSYLTIDLTDDTEDTHGTPQSSSTPYARLAQ DSQQGGMESPQVEADSGDGDSSEYQDGKEFGIG DLVWGKIKGFSWWPAMVVSWKATSKRQAMSGMRW VQWFGDGKFSEVSADKLVALGLFSQHFNLATFN KLVSYRKAMYHALEKARVRAGKTFPSSPGDSLE DQLKPMLEWAHGGFKPTGIEGLKPNNTQPVVNKS KVRRAGSRKLESRKYENKTRRRTADDSATSDYC PAPKRLKTNCYNNGKDRGDEDQSREQMASDVAN NKSSLEDGCLSCGRKNPVSFHPLFEGGLCQTCRD RFLELFYMYDDDGYQSYCTVCCEGRELLLCSNT SCCRCFCVECLEVLVGTGTAAEAKLQEPWSCYM CLPQRCHGVLRRRKDWNVRLQAFFTSDTGLEYEA PKLYPAIPAARRRPIRVLSLFDGIATGYLVLKE LGIKVGKYVASEVCEESIAVGTVKHEGNIKYVN DVRNITKKNIEEWGPFDLVIGGSPCNDLSNVNPA RKGLYEGTGRLFFEFYHLLNYSRPKEGDDRPFF WMFENVVAMKVGDKRDISRFLECNPVMIDAIKV SAAHRARYFWGNLPGMNRPVIASKNDKLELQDCL EYNRIAKLKKVQTITTKSNSIKQGKNQLFPVVM NGKEDVLWCTELERIFGFPVHYTDVSNMGRGAR QKLLGRSWSVPVIRHLFAPLKDYFACE*FQPGPK PTGVCGRARTQEV*FLKASPGPALPQLCGSYRV PQFPLAQWGQSHLTLAGVA*GAASLCTNQTWLL GAA*HGAHFFFS*NFKT*SR*QRGFFFFPSWVYH SEKQWLRYQNHSADSSPILRLMLKNHPRQLLQE FNF*KLATALCLQTCAVVGM*LQDIFKGPGSFF PRASRRENVVYVFYPAHSPCLNTRAGVCTGPIRV FSTMMMISAGMTSSSHSGLFFPPQTQGQGPLLA KSLPVTAIEPSGELRKGCAEFYNISCHIFCRQV WLLHISLFPRRGV*SKELR*DTPSNPFPLQETYP PQAQVPR*EVCYPHFSSFY*TQRQ*QQSGTDIH FSYLPHI*EMTGKTAKLGAPFGDFLILFYSIRS RF*GEREFRQAAFQKCCHNGF*HLLLFLLVLFCR IRNNVDKFCGAFYTLFKISNFYFYV*RFH*NFF CNWSHDVTNMGKKLCLVSTVFANF*AER*RMPR VYLMFN*NQYLSI

Frame 1

PTPAAPSGPRRFLAAAAPGSAAAARVDAPSAPR RTGPAPWRSGERAATLPGRQRWG*VAQVNLARR SAPEIREPSALHGRQPASRQPAPTRGSAAQPRPS QPCGRKA*RETPGISMERRTPAGGKTRSSSTGP AATSPPTRPQSWRLSAPRRSEAEDQARDSPRGR CPVC*ATHRT*QAMATGKMGMALTPQSCQSSSGK PGLVQKAQLSELEITTVSPAGRGTGLPHVPPEA GRAATMWTSPPWSSRLPGP*DGGQQHRQERHGR PLPALTLPSTSQTTQRTHMGRPRAAVPPTPA*PR TASRGAWSPRRWRQTVEMETVQSIRMGRSLE*G TSCGERSRASPGGPPWWCLGRPPPSDRLCLACG GSSGLAMASSPRSLQTNWWHWGCSASTLIWPPSI SSSPIEKPCTMLWRKLGCELARPSPAALETHWR TS*SPCWSGPTGASSPLGSRASNPTTRNQWLIS RRCVVQAVGN*NQGNTRTRLEDAQLTTQPPLTTA PHPSASRQIAITTAKTEGMKIRAENKWLQMLPT TRAAWKMAVCLVAGKTPCPSTLSLRGGSVRHAG IASLSCFTCMMTMAISLTALCAARAESCCFAATR AAAGVSVWSAWRCWWAQAQRPRPSFRSPGAVTC VSRSAVMASCGAGRTGTCACRPSSPVTRGLNMK PPSCTLPFPQPEGGPFESCHCLMASRQAT*SSKS WA*R*ESTSLLKCVRSPLLLEP*STRGISNT*T T*GTSQRKILKNGAHLTW*LAEAHATISQM*IQ PGKACMRVQAGSSSNFTTC*ITHAPRRVMTGRSS GCLRML*P*RLATRGTSHGSWSVIQ**LMPSKF LLLTGPDTSGATYPG*TGP**HQRMINSSCRTA WNTIG*PS*RKYRQ*PPSRTRSNRGKTNFSLLS* MAKKMFCGALSSKGSLAFLCTTQTCPTWAVVPA RSCWEGPGACLSSDTSSPL*RTTLHVNSSSQAP SPLGCVAEPGPRRCDS*RHPQALLFLSCVGHTVY LSSLLLSGGRAT*LLQG*PEVPPPCAQIRPGCL EQPNTVLIFSSPKTLKLEVGSNVAFFFSLPGST TQRNNG*DTKTTVPTALQYSG*C*KIIQDSYCKS LIFENWLLLCVYRRVQL*ACSYRTFLRAQDRFF PGQAEEKMLYMSFTRHIPLA*IQGLESARDLLE YFPQ***FQQG*RHHHIQGYFFPHKPKGRGHS*L NPSP*LQ*NPLGSSGRGVLSSII*AAIYFVDKY GSSISPSSLGEECEARSLDKTPPQTHSLSRRPT LHRHRSPDEKSATLISHLFTKLRGSDSSQGQTYI SHTFPTSER*QGKLQSSVLPLEIF*SFFIP*EV VFRENGNSDKLHFRNAVIMVFNTFYSSYWCYFV E*GTTLTSFVGLFIHFLKSQTSIFMFNVFIKIFF VTGATT*QIWGKNCALFQQFLLIFRLKDDGCLE FTLCLIKISICL*

Frame 2

PLPLPRPARAASSQQLLPAPRPQPAWTLRAPPD GRDRLPGGRASGRQRCPAGSAGVKWPK*T*LGD RRRRFASPAPCTAASRPPASQPRPAAPPPSRAP ASPAAGKHEGRHQASQWRGGRRREGRLDPRQRGL QRPVLRLAPNPGGYPHPGDQRPKIKLATLQEGG VQSAKLHTGLDRRWRRGRWGWL*HPSHAKALPG NQDSFRKPSCPNSK*QQCLQPGEAQAFPTFHPRP AGPQPCGRVPRGVPGYQVPETAGNSIGRNAMAV PSQLLPYHRPHRRHRGHTWDAPEQQYPLRPPSP GQPAGGHGVPAGGGRQWRWRQFRVSGWEGVWNRG PRVGKDQGLLLVARHGGVLEGHLQATGYVWHAV GPVVWRWQVLRGLCRQTGGTGAVQPAL*FGHLQ *ARLLSKSHVPCSGES*GASWQDLPQQPWRLIGG PAEAHVGVGPRGLQAHWDRGPQTQQHATSG**V EGASCRQ*EIRIKEIREQDSKTHS*RLSHL*LL PRTQAPQDKLL*QRQRPRG*RSEPRTNGFRCCQQ QEQPGRWLFVLWQEKPRVLPPSL*GGALSDMPG SLP*AVLHV**RWLSVLLHCVLRGPRAAALQQH ELLPVFLCGVPGGAGGHRHSGRGQASGALELLHV SPAALSWRPAAPEGLERAPAGLLHQ*HGA*I*S PQAVPCHSRSPKAAHSSPVIV*WHRDRLPSPQR VGHKGRKVRRF*SV*GVHCCWNREARGEYQIRER REEHHKEKY*RMGPI*LGDWRKPMQRSLKCESS QERPV*GYRPALLRILPPAELLTPQGG**PAVL LDV*ECCSHEGWRQEGHLTVPGV*SSDD*CHQSF CCSQGPILLGQPTRDEQARDSIKE**TRAAGLL GIQ*DSQVKESTDNNHQVELDQTGEKPTFPCCH EWQRRCFVVH*ARKDLWLSCALHRRVQHGPWCPP EAAGKVLERACHPTPLRPSEGLLCM*IVPARPQ AHWGVWQSQDPGGVIPEGIPRPCSSSAVWVIPC TSVPSCSVGAEPPDSCRGSLRCRLLVHKSDLAAW SSLTRCSFFLLLKL*NLK*VATWLFFFPFLGLP LRETMAKIPKPQCRQLSNTQVNAEKSSKTVIAR V*FLKTGYCSVFTDVCSCRHVATGHF*GPRIVFS QGKQKRKCCICLLPGTFPLPKYKGWSLHGTY*S IFHNDDDFSRDDVIITFRAIFSPTNPRAGATLS *IPPRDCNRTLWGAQEGVC*VL*YKLPYIL*TSM APPYLPLP*ERSVKQGA*IRHPLKPIPSPGDLP STGTGPQMRSLLPSFLIFLLNSEAVTAVRDRHT FLIPSPHLRDDRENCKARCSLWRFFNPFLFHKKS FLGRTGIQTSCISEMLS*WFLTPFTLLTGAIL* NKEQR*QVLWGFLYTF*NLKLLFLCLTFSLKFF L*LEPRRNKYGEKTVPCFNSFC*FLG*KMTDA*S LPYV*LKSVFVY