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Sequences

Sequence CASC4.bAug05

ACATCCGCCCCTTTCCGGTTTTTTTCCCCGCCTCCCAACCGTGAGGTGTTGGGTTTGGGGGACGCTGGCAGCTGGGTTCTCCCGGTTCCCTTGGGCAGGT
GCAGGGTCGGGTTCAAAGCCTCCGGAACGCGTTTTGGCCTGATTTGAGGAGGGGGGCGGGGAGGGACCTGCGGCTTGCGGCCCCGCCCCCTTCTCCGGCT
CGCAGCCGACCGGTAAGCCCGCCTCCTCCCTCGGCCGGCCCTGGGGCCGTGTCCGCCGGGCAACTCCAGCCGAGGCCTGGGCTTCTGCCTGCAGGTGTCT
GCGGCGAGGCCCCTAGGGTACAGCCCGATTTGGCCCCATGGTGGGTTTCGGGGCCAACCGGCGGGCTGGCCGCCTGCCCTCTCTCGTGCTGGTGGTGCTG
CTGGTGGTGATCGTCGTCCTCGCCTTCAACTACTGGAGCATCTCCTCCCGCCACGTCCTGCTTCAGGAGGAGGTGGCCGAGCTGCAGGGCCAGGTCCAGC
GCACCGAAGTGGCCCGCGGGCGGCTGGAAAAGCGCAATTCGGACCTCTTGCTGTTGGTGGACACGCACAAGAAACAGATCGACCAGAAGGAGGCCGACTA
CGGCCGCCTCAGCAGCCGGCTGCAGGCCAGAGAGGGCCTCGGGAAGAGATGCGAGGATGACAAGGTTAAACTACAGAACAACATATCGTATCAGATGGCA
GACATACATCATTTAAAGGAGCAACTTGCTGAGCTTCGTCAGGAATTTCTTCGACAAGAAGACCAGCTTCAGGACTATAGGAAGAACAATACTTACCTTG
TGAAGAGGTTAGAATATGAAAGTTTTCAGTGTGGACAGCAGATGAAGGAATTGAGAGCACAGCATGAAGAAAATATTAAAAAGTTAGCAGACCAGTTTTT
AGAGGAACAAAAGCAAGAGACCCAAAAGATTCAATCAAATGATGGAAAGGAATTGGATATAAACAATCAAGTAGTACCTAAAAATATTCCAAAAGTAGCT
GAGAATGTTGCAGATAAGAATGAAGAACCCTCAAGCAATCATATTCCACATGGGAAAGAACAAATCAAAAGAGGTGGTGATGCAGGGATGCCTGGAATAG
AAGAGAATGACCTAGCAAAAGTTGATGATCTTCCCCCTGCTTTAAGGAAGCCTCCTATTTCAGTTTCTCAACATGAAAGTCATCAAGCAATCTCCCATCT
TCCAACTGGACAACCTCTCTCCCCAAATATGCCTCCAGATTCACACATAAACCACAATGGAAACCCCGGTACTTCAAAACAGAATCCTTCCAGTCCTCTT
CAGCGTTTAATTCCAGGCTCAAACTTGGACAGTGAACCCAGAATTCAAACAGATATACTAAAGCAGGCTACCAAGGACAGAGTCAGTGATTTCCATAAAT
TGAAGCAAAATGATGAAGAACGAGAGCTTCAAATGGATCCTGCAGACTATGGAAAGCAACATTTCAATGATGTCCTTTAAGTCCTAAAGGAATGCTTCAG
AAAACCTAAAGTGCTGTAAAATGAAATCATTCTACTTTGTCCTTTCTGACTTTTGTTGTAAAGACGAATTGTATCAGTTGTAAAGATACATTGAGATAGA
ATTAAGGAAAAACTTTAATGAAGGAATGTACCCATGTACATATGTGAACTTTTTCATATTGTATTATCAAGGTATAGACTTTTTTGGTTATGATACAGTT
AAGCCAAAAACAGCTAATCTTTGCATCTAAAGCAAACTAATGTATATTTCACATTTTATTGAGCCGACTTATTTCCACAAATAGATAAACAGGACAAAAT
AGTTGTACAGGTTATATGTGGCATAGCATAACCACAGTAAGAACAGAACAGATATTCAGCAGAAAACTTTTTATACTCTAATTCTTTTTTTTTTTTTTTT
TGAGACAGAGTTTTAGTCTTGTTTCCCAGGCTGGAGTGCAATGGCACAATCTTGGCTCACTGCAACCTCCGCCTCCTGGGTTCAGGCAATTTTCCTGCCT
CAGCCTCCCAAGTAGCTGGGATTACAGGCACCCACCACCATGCCCAGCTAATTTTTGTATTTTTAATAGAGAGCTAATAATTGTATATTTAATAAAGACG
GGTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCAGGTGATCCTCCTGCATTGGCCTCCCAAAGTGCTGGAATTCCAGGCATGAGCCACTG
CGCCCAGTCTACACACTAATTCTTGTTAGCCCAACAGCTGTTCTGTTCTATCTACCCCTCATTTCACGCTCAAGGAGTCATACCTAGAATAGTTACACAC
AAGAGGGAAACTGGAAGCCAAACACTGTACAGTATTGTGTAGAAAGTCACCTCCCTACTCCTTTTATTTTACATGAGTGCTGATGTGTTTTGGCAGATGA
GCTTTCAGCTGAGGCCTGATGGAAATTGAGATAACCTGCAAAGACATAACAGTATTTATGAGTTATATCTTAGTTCTTGAAATTGTGGAATGCATGATTG
ACAATATATTTTTAATTTTTATTTTTTCAAGTAATACCAGTACTGTTTAACTATAGCCAGAACTGGCTAAAATTTTTATATTTTCAGAGTTGAAGTTGGT
GAAGACATTCATGATTTAAACACCAGATCCTGAAAGGGGTTAAATCTACTTTGAAATGAATCTGCAATCAGTATTTCAAAGCTTTTCTGGTAATTTTAGT
GATCTTATTTGATTAGACTTTTTCAGAAGTACTAAATAAGGAATTTTAACAGGTTTTTATTAATGCACAGATAAATAGAAGTACAGTGAGGTCTATAGCC
ATTTTATTAAAATAGCTTAAAAGTTTGTAAAAAAATGAATCTTTGTAATTACTTAATATGTTAGTTAAGAACCCGTCAAGCTTATATTTGCTAGACTTAC
AAATTATTTTAAATGCATTTATCTTTTTTGACACTATTCAGTGGAATGTGTAAGCTAGCTAATTCTTGTTTTCTGATTTAAAGCACTTTTAAATCTTATC
CTGCCCCCTAAAAACAAAAGGTTTTGATCACAAGGGGAAATTTAAGATTGTTAACCCTGTTTTTCAGAAGGGCTACTGTTAATTGCACATAAACATGAAA
TGTGTTTTCCCCTGTGTACTAACACATTCTAGGCAAAATTCAAACTTATAGTGGTAAAGAAACAGGTTGTTCACTTGCTGAGGTGCAAAAATTCTTAAGA
CTTCTGTTTGAAATTGCTCAATGACTAGGAAAAGATGTAGTAGTTTACTAAAATTGTTTTTCTACCATATCAAATTAAACAATTCATGCCTTTATAGGGT
CAGGCCTACAATGAATAGGTATGGTGGTTTCACAGAATTTTAAAATAGAGTTAAAGGGAAGTGATGTACATTTCGGGGGCATTAGGGTAGGGAGATGAAT
CAAAAAATACCCCTAGTAATGCTTTATATTTTAATACTGCAAAAGCTTTACAAATGGAAACCATGCAATTACCTGCCTTAGTTCTTTTGTCATAAAAACA
ATCACTTGGTTGGTTGTATTGTAGCTATTACTTATACAGCAACATTTCTTCAATTAGCAGTCTAGACATTTTATAAACAGAAATCTTGGACCAATTGATA
ATATTTCTGACTGTATTAATATTTTAGTGCTATAAAATACTATGTGAATCTCTTAAAAATCTGACATTTTACAGTCTGTATTAGACATACTGTTTTTATA
ATGTTTTACTTCTGCCTTAAGATTTAGGTTTTTTAAATGTATTTTTGCCCTGAATTAAGTGTTAATTTGATGGAAACTCTGCTTTTAAAATCATCATTTA
CTGGGTTCTAATAAATTAAAAATTAAACTTGTTTC
  • CASC4.F1 (forward primer)
  • NM_177974-R1 (reverse primer)
  • Single exon skipping

Frame 0

TSAPFRFFSPPPNREVLGLGDAGSWVLPVPLGR
CRVGFKASGTRFGLI*GGGRGGTCGLRPRPLLRL
AADR*ARLLPRPALGPCPPGNSSRGLGFCLQVS
AARPLGYSPIWPHGGFRGQPAGWPPALSRAGGA
AGGDRRPRLQLLEHLLPPRPASGGGGRAAGPGPA
HRSGPRAAGKAQFGPLAVGGHAQETDRPEGGRL
RPPQQPAAGQRGPREEMRG*QG*TTEQHIVSDG
RHTSFKGATC*ASSGISSTRRPASGL*EEQYLPC
EEVRI*KFSVWTADEGIESTA*RKY*KVSRPVF
RGTKARDPKDSIK*WKGIGYKQSSST*KYSKSS
*ECCR*E*RTLKQSYSTWERTNQKRW*CRDAWNR
RE*PSKS**SSPCFKEASYFSFST*KSSSNLPS
SNWTTSLPKYASRFTHKPQWKPRYFKTESFQSS
SAFNSRLKLGQ*TQNSNRYTKAGYQGQSQ*FP*I
EAK**RTRASNGSCRLWKATFQ*CPLSPKGMLQ
KT*SAVK*NHSTLSFLTFVVKTNCISCKDTLR*
N*GKTLMKECTHVHM*TFSYCIIKV*TFLVMIQL
SQKQLIFASKAN*CIFHILLSRLISTNR*TGQN
SCTGYMWHSITTVRTEQIFSRKLFIL*FFFFFF
LRQSFSLVSQAGVQWHNLGSLQPPPPGFRQFSCL
SLPSSWDYRHPPPCPANFCIFNRELIIVYLIKT
GFTMLARLVLNS*PQVILLHWPPKVLEFQA*AT
APSLHTNSC*PNSCSVLSTPHFTLKESYLE*LHT
RGKLEAKHCTVLCRKSPPYSFYFT*VLMCFGR*
AFS*GLMEIEITCKDITVFMSYILVLEIVECMI
DNIFLIFIFSSNTSTV*L*PELAKIFIFSELKLV
KTFMI*TPDPERG*IYFEMNLQSVFQSFSGNFS
DLI*LDFFRSTK*GILTGFY*CTDK*KYSEVYS
HFIKIA*KFVKK*IFVIT*YVS*EPVKLIFARLT
NYFKCIYLF*HYSVECVS*LILVF*FKALLNLI
LPPKNKRF*SQGEI*DC*PCFSEGLLLIAHKHE
MCFPLCTNTF*AKFKLIVVKKQVVHLLRCKNS*D
FCLKLLND*EKM**FTKIVFLPYQIKQFMPL*G
QAYNE*VWWFHRILK*S*REVMYISGALG*GDE
SKNTPSNALYFNTAKALQMETMQLPALVLLS*KQ
SLGWLYCSYYLYSNISSISSLDIL*TEILDQLI
IFLTVLIF*CYKILCESLKNLTFYSLY*TYCFY
NVLLLP*DLGFLNVFLP*IKC*FDGNSAFKIIIY
WVLIN*KLNLF

Frame 1

HPPLSGFFPRLPTVRCWVWGTLAAGFSRFPWAG
AGSGSKPPERVLA*FEEGGGEGPAACGPAPFSG
SQPTGKPASSLGRPWGRVRRATPAEAWASACRCL
RRGP*GTARFGPMVGFGANRRAGRLPSLVLVVL
LVVIVVLAFNYWSISSRHVLLQEEVAELQGQVQ
RTEVARGRLEKRNSDLLLLVDTHKKQIDQKEADY
GRLSSRLQAREGLGKRCEDDKVKLQNNISYQMA
DIHHLKEQLAELRQEFLRQEDQLQDYRKNNTYL
VKRLEYESFQCGQQMKELRAQHEENIKKLADQFL
EEQKQETQKIQSNDGKELDINNQVVPKNIPKVA
ENVADKNEEPSSNHIPHGKEQIKRGGDAGMPGI
EENDLAKVDDLPPALRKPPISVSQHESHQAISHL
PTGQPLSPNMPPDSHINHNGNPGTSKQNPSSPL
QRLIPGSNLDSEPRIQTDILKQATKDRVSDFHK
LKQNDEERELQMDPADYGKQHFNDVL*VLKECFR
KPKVL*NEIILLCPF*LLL*RRIVSVVKIH*DR
IKEKL**RNVPMYICELFHIVLSRYRLFWL*YS
*AKNS*SLHLKQTNVYFTFY*ADLFPQIDKQDKI
VVQVICGIA*PQ*EQNRYSAENFLYSNSFFFFF
*DRVLVLFPRLECNGTILAHCNLRLLGSGNFPA
SASQVAGITGTHHHAQLIFVFLIES**LYI**RR
VSPCWPGWS*TPDLR*SSCIGLPKCWNSRHEPL
RPVYTLILVSPTAVLFYLPLISRSRSHT*NSYT
QEGNWKPNTVQYCVESHLPTPFILHEC*CVLADE
LSAEA*WKLR*PAKT*QYL*VIS*FLKLWNA*L
TIYF*FLFFQVIPVLFNYSQNWLKFLYFQS*SW
*RHS*FKHQILKGVKSTLK*ICNQYFKAFLVILV
ILFD*TFSEVLNKEF*QVFINAQINRSTVRSIA
ILLK*LKSL*KNESL*LLNMLVKNPSSLYLLDL
QIILNAFIFFDTIQWNV*AS*FLFSDLKHF*ILS
CPLKTKGFDHKGKFKIVNPVFQKGYC*LHINMK
CVFPCVLTHSRQNSNL*W*RNRLFTC*GAKILK
TSV*NCSMTRKRCSSLLKLFFYHIKLNNSCLYRV
RPTMNRYGGFTEF*NRVKGK*CTFRGH*GREMN
QKIPLVMLYILILQKLYKWKPCNYLP*FFCHKN
NHLVGCIVAITYTATFLQLAV*TFYKQKSWTN**
YF*LY*YFSAIKYYVNLLKI*HFTVCIRHTVFI
MFYFCLKI*VF*MYFCPELSVNLMETLLLKSSF
TGF**IKN*TCF

Frame 2

IRPFPVFFPASQP*GVGFGGRWQLGSPGSLGQV
QGRVQSLRNAFWPDLRRGAGRDLRLAAPPPSPA
RSRPVSPPPPSAGPGAVSAGQLQPRPGLLPAGV
CGEAPRVQPDLAPWWVSGPTGGLAACPLSCWWCC
WW*SSSSPSTTGASPPATSCFRRRWPSCRARSS
APKWPAGGWKSAIRTSCCWWTRTRNRSTRRRPT
TAASAAGCRPERASGRDARMTRLNYRTTYRIRWQ
TYII*RSNLLSFVRNFFDKKTSFRTIGRTILTL
*RG*NMKVFSVDSR*RN*EHSMKKILKS*QTSF
*RNKSKRPKRFNQMMERNWI*TIK*YLKIFQK*L
RMLQIRMKNPQAIIFHMGKNKSKEVVMQGCLE*
KRMT*QKLMIFPLL*GSLLFQFLNMKVIKQSPI
FQLDNLSPQICLQIHT*TTMETPVLQNRILPVLF
SV*FQAQTWTVNPEFKQIY*SRLPRTESVISIN
*SKMMKNESFKWILQTMESNISMMSFKS*RNAS
ENLKCCKMKSFYFVLSDFCCKDELYQL*RYIEIE
LRKNFNEGMYPCTYVNFFILYYQGIDFFGYDTV
KPKTANLCI*SKLMYISHFIEPTYFHK*INRTK
*LYRLYVA*HNHSKNRTDIQQKTFYTLILFFFFF
ETEF*SCFPGWSAMAQSWLTATSASWVQAIFLP
QPPK*LGLQAPTTMPS*FLYF**RANNCIFNKD
GFHHVGQAGLELLTSGDPPALASQSAGIPGMSHC
AQSTH*FLLAQQLFCSIYPSFHAQGVIPRIVTH
KRETGSQTLYSIV*KVTSLLLLFYMSADVFWQM
SFQLRPDGN*DNLQRHNSIYELYLSS*NCGMHD*
QYIFNFYFFK*YQYCLTIARTG*NFYIFRVEVG
EDIHDLNTRS*KGLNLL*NESAISISKLFW*F*
*SYLIRLFQKY*IRNFNRFLLMHR*IEVQ*GL*P
FY*NSLKVCKKMNLCNYLIC*LRTRQAYIC*TY
KLF*MHLSFLTLFSGMCKLANSCFLI*STFKSY
PAP*KQKVLITRGNLRLLTLFFRRATVNCT*T*N
VFSPVY*HILGKIQTYSGKETGCSLAEVQKFLR
LLFEIAQ*LGKDVVVY*NCFSTISN*TIHAFIG
SGLQ*IGMVVSQNFKIELKGSDVHFGGIRVGR*I
KKYP**CFIF*YCKSFTNGNHAITCLSSFVIKT
ITWLVVL*LLLIQQHFFN*QSRHFINRNLGPID
NISDCINILVL*NTM*IS*KSDILQSVLDILFL*
CFTSALRFRFFKCIFALN*VLI*WKLCF*NHHL
LGSNKLKIKLV

Sequence CASC4.dAug05

AAAAAAAAAAATGGCTAGGCATAGTGGCACACACCTGTAGTCCTAGCTACTTGGGAAGCTGAGGTGGGAGGATCACATGAGCCCAGGAGTTCGAGATTGC
AGTGAACTGTGATTGCACCACTGTACTCCAGTCTGGGTGACAGAGAGAGACCCTGTCTAAACAAAAAAGAGAGAGAGAGAGAGAGAGAGGAGGCAGAGTG
AGATAACTGAATAGAAGCCTCCACTGATTGTCCTCCCTGCAGTAGCACCAAATTTGACAACTGTCTACACAGAAAAGTACCTTCATGAGAGCCAAAAATC
AGATGTCATTATCTTATATGTAGAAGACCCAAGAGAACTGACAAAAACGCTCCTGGAACTACTTTAAGGAAGCCTCCTATTTCAGTTTCTCAACATGAAA
GTCATCAAGCAATCTCCCATCTTCCAACTGGACAACCTCTCTCCCCAAATATGCCTCCAGATTCACACATAAACCACAATGGAAACCCCGGTACTTCAAA
ACAGAATCCTTCCAGTCCTCTTCAGCGTTTAATTCCAGGCTCAAACTTGGACAGTGAACCCAGAATTCAAACAGATATACTAAAGCAGGCTACCAAGGAC
AGAGTCAGTGATTTCCATAAATTGAAGCAAAGCCGATTCTTTGATGAAAATGAATCCCCTGTTGATCCGCAGCATGGCTCTAAACTGGCGGATTATAATG
GGGATGATGGTAACGTAGGTGAGTATGAGGCAGACAAGCAGGCTGAGCTGGCTTACAATGAGGAAGAAGATGGTGATGGTGGAGAGGAAGACGTCCAAGA
TGATGAAGAACGAGAGCTTCAAATGGATCCTGCAGACTATGGAAAGCAACATTTCAATGATGTCCTTTAAGTCCTAAAGGAATGCTTCAGAAAACCTAAA
AGTTGAAGTTGGTGAAGACATTCATGATTTAAACACCAGATCCTGAAAGGGGTTAAATCTACTTTGAAATGAATCTGCAATCAGTATTTCAAAGCTTTTC
TGGTAATTTTAGTGATCTTATTTGATTAGACTTTTTCAGAAGTACTAAATAAGGAATTTTAACAGGTTTTTATTAATGCACAGATAAATAGAAGTACAGT
GAGGTCTATAGCCATTTTATTAAAATAGCTTAAAAGTTTGTAAAAAAATGAATCTTTGTAATTACTTAATATGTTAGTTAAGAACCCGTCAAGCTTATAT
TTGCTAGACTTACAAATTATTTTAAATGCATTTATCTTTTTTGACACTATTCAGTGGAATGTGTAAGCTAGCTAATTCTTGTTTTCTGATTTAAAGCACT
TTTAAATCTTATCCTGCCCCCTAAAAACAAAAGGTTTTGATCACAAGGGGAAATTTAAGATTGTTAACCCTGTTTTTCAGAAGGGCTACTGTTAATTGCA
CATAAACATGAAATGTGTTTTCCCCTGTGTACTAACACATTCTAGGCAAAATTCAAACTTATAGTGGTAAAGAAACAGGTTGTTCACTTGCTGAGGTGCA
AAAATTCTTAAGACTTCTGTTTGAAATTGCTCAATGACTAGGAAAAGATGTAGTAGTTTACTAAAATTGTTTTTCTACCATATCAAATTAAACAATTCAT
GCCTTTATAGGGTCAGGCCTACAATGAATAGGTATGGTGGTTTCACAGAATTTTAAAATAGAGTTAAAGGGAAGTGATGTACATTTCGGGGGCATTAGGG
TAGGGAGATGAATCAAAAAATACCCCTAGTAATGCTTTATATTTTAATACTGCAAAAGCTTTACAAATGGAAACCATGCAATTACCTGCCTTAGTTCTTT
TGTCATAAAAACAATCACTTGGTTGGTTGTATTGTAGCTATTACTTATACAGCAACATTTCTTCAATTAGCAGTCTAGACATTTTATAAACAGAAATCTT
GGACCAATTGATAATATTTCTGACTGTATTAATATTTTAGTGCTATAAAATACTATGTGAATCTCTTAAAAATCTGACATTTTACAGTCTGTATTAGACA
TACTGTTTTTATAATGTTTTACTTCTGCCTTAAGATTTAGGTTTTTTAAATGTATTTTTGCCCTGAATTAAGTGTTAATTTGATGGAAACTCTGCTTTTA
AAATCATCATTTACTGGGTTCTAATAAATTAAAAATTAAACTTGTTTC
  • CASC4.F1 (forward primer)
  • NM_177974-R1 (reverse primer)
  • Single exon skipping

Frame 0

KKKNG*A*WHTPVVLATWEAEVGGSHEPRSSRL
Q*TVIAPLYSSLGDRERPCLNKKEREREREEAE*
DN*IEASTDCPPCSSTKFDNCLHRKVPS*EPKI
RCHYLICRRPKRTDKNAPGTTLRKPPISVSQHE
SHQAISHLPTGQPLSPNMPPDSHINHNGNPGTSK
QNPSSPLQRLIPGSNLDSEPRIQTDILKQATKD
RVSDFHKLKQSRFFDENESPVDPQHGSKLADYN
GDDGNVGEYEADKQAELAYNEEEDGDGGEEDVQD
DEERELQMDPADYGKQHFNDVL*VLKECFRKPK
S*SW*RHS*FKHQILKGVKSTLK*ICNQYFKAF
LVILVILFD*TFSEVLNKEF*QVFINAQINRSTV
RSIAILLK*LKSL*KNESL*LLNMLVKNPSSLY
LLDLQIILNAFIFFDTIQWNV*AS*FLFSDLKH
F*ILSCPLKTKGFDHKGKFKIVNPVFQKGYC*LH
INMKCVFPCVLTHSRQNSNL*W*RNRLFTC*GA
KILKTSV*NCSMTRKRCSSLLKLFFYHIKLNNS
CLYRVRPTMNRYGGFTEF*NRVKGK*CTFRGH*G
REMNQKIPLVMLYILILQKLYKWKPCNYLP*FF
CHKNNHLVGCIVAITYTATFLQLAV*TFYKQKS
WTN**YF*LY*YFSAIKYYVNLLKI*HFTVCIRH
TVFIMFYFCLKI*VF*MYFCPELSVNLMETLLL
KSSFTGF**IKN*TCF

Frame 1

KKKMARHSGTHL*S*LLGKLRWEDHMSPGVRDC
SEL*LHHCTPVWVTERDPV*TKKRERERERRQS
EITE*KPPLIVLPAVAPNLTTVYTEKYLHESQKS
DVIILYVEDPRELTKTLLELL*GSLLFQFLNMK
VIKQSPIFQLDNLSPQICLQIHT*TTMETPVLQ
NRILPVLFSV*FQAQTWTVNPEFKQIY*SRLPRT
ESVISIN*SKADSLMKMNPLLIRSMALNWRIIM
GMMVT*VSMRQTSRLSWLTMRKKMVMVERKTSK
MMKNESFKWILQTMESNISMMSFKS*RNASENLK
VEVGEDIHDLNTRS*KGLNLL*NESAISISKLF
W*F**SYLIRLFQKY*IRNFNRFLLMHR*IEVQ
*GL*PFY*NSLKVCKKMNLCNYLIC*LRTRQAYI
C*TYKLF*MHLSFLTLFSGMCKLANSCFLI*ST
FKSYPAP*KQKVLITRGNLRLLTLFFRRATVNC
T*T*NVFSPVY*HILGKIQTYSGKETGCSLAEVQ
KFLRLLFEIAQ*LGKDVVVY*NCFSTISN*TIH
AFIGSGLQ*IGMVVSQNFKIELKGSDVHFGGIR
VGR*IKKYP**CFIF*YCKSFTNGNHAITCLSSF
VIKTITWLVVL*LLLIQQHFFN*QSRHFINRNL
GPIDNISDCINILVL*NTM*IS*KSDILQSVLD
ILFL*CFTSALRFRFFKCIFALN*VLI*WKLCF*
NHHLLGSNKLKIKLV

Frame 2

KKKWLGIVAHTCSPSYLGS*GGRIT*AQEFEIA
VNCDCTTVLQSG*QRETLSKQKRERERERGGRV
R*LNRSLH*LSSLQ*HQI*QLSTQKSTFMRAKN
QMSLSYM*KTQEN*QKRSWNYFKEASYFSFST*K
SSSNLPSSNWTTSLPKYASRFTHKPQWKPRYFK
TESFQSSSAFNSRLKLGQ*TQNSNRYTKAGYQG
QSQ*FP*IEAKPIL**K*IPC*SAAWL*TGGL*W
G*W*RR*V*GRQAG*AGLQ*GRRW*WWRGRRPR
**RTRASNGSCRLWKATFQ*CPLSPKGMLQKT*
KLKLVKTFMI*TPDPERG*IYFEMNLQSVFQSFS
GNFSDLI*LDFFRSTK*GILTGFY*CTDK*KYS
EVYSHFIKIA*KFVKK*IFVIT*YVS*EPVKLI
FARLTNYFKCIYLF*HYSVECVS*LILVF*FKAL
LNLILPPKNKRF*SQGEI*DC*PCFSEGLLLIA
HKHEMCFPLCTNTF*AKFKLIVVKKQVVHLLRC
KNS*DFCLKLLND*EKM**FTKIVFLPYQIKQFM
PL*GQAYNE*VWWFHRILK*S*REVMYISGALG
*GDESKNTPSNALYFNTAKALQMETMQLPALVL
LS*KQSLGWLYCSYYLYSNISSISSLDIL*TEIL
DQLIIFLTVLIF*CYKILCESLKNLTFYSLY*T
YCFYNVLLLP*DLGFLNVFLP*IKC*FDGNSAF
KIIIYWVLIN*KLNLF

Sequence CASC4.aAug05

ACATCCGCCCCTTTCCGGTTTTTTTCCCCGCCTCCCAACCGTGAGGTGTTGGGTTTGGGGGACGCTGGCAGCTGGGTTCTCCCGGTTCCCTTGGGCAGGT
GCAGGGTCGGGTTCAAAGCCTCCGGAACGCGTTTTGGCCTGATTTGAGGAGGGGGGCGGGGAGGGACCTGCGGCTTGCGGCCCCGCCCCCTTCTCCGGCT
CGCAGCCGACCGGTAAGCCCGCCTCCTCCCTCGGCCGGCCCTGGGGCCGTGTCCGCCGGGCAACTCCAGCCGAGGCCTGGGCTTCTGCCTGCAGGTGTCT
GCGGCGAGGCCCCTAGGGTACAGCCCGATTTGGCCCCATGGTGGGTTTCGGGGCCAACCGGCGGGCTGGCCGCCTGCCCTCTCTCGTGCTGGTGGTGCTG
CTGGTGGTGATCGTCGTCCTCGCCTTCAACTACTGGAGCATCTCCTCCCGCCACGTCCTGCTTCAGGAGGAGGTGGCCGAGCTGCAGGGCCAGGTCCAGC
GCACCGAAGTGGCCCGCGGGCGGCTGGAAAAGCGCAATTCGGACCTCTTGCTGTTGGTGGACACGCACAAGAAACAGATCGACCAGAAGGAGGCCGACTA
CGGCCGCCTCAGCAGCCGGCTGCAGGCCAGAGAGGGCCTCGGGAAGAGATGCGAGGATGACAAGGTTAAACTACAGAACAACATATCGTATCAGATGGCA
GACATACATCATTTAAAGGAGCAACTTGCTGAGCTTCGTCAGGAATTTCTTCGACAAGAAGACCAGCTTCAGGACTATAGGAAGAACAATACTTACCTTG
TGAAGAGGTTAGAATATGAAAGTTTTCAGTGTGGACAGCAGATGAAGGAATTGAGAGCACAGCATGAAGAAAATATTAAAAAGTTAGCAGACCAGTTTTT
AGAGGAACAAAAGCAAGAGACCCAAAAGATTCAATCAAATGATGGAAAGGAATTGGATATAAACAATCAAGTAGTACCTAAAAATATTCCAAAAGTAGCT
GAGAATGTTGCAGATAAGAATGAAGAACCCTCAAGCAATCATATTCCACATGGGAAAGAACAAATCAAAAGAGGTGGTGATGCAGGGATGCCTGGAATAG
AAGAGAATGACCTAGCAAAAGTTGATGATCTTCCCCCTGCTTTAAGGAAGCCTCCTATTTCAGTTTCTCAACATGAAAGTCATCAAGCAATCTCCCATCT
TCCAACTGGACAACCTCTCTCCCCAAATATGCCTCCAGATTCACACATAAACCACAATGGAAACCCCGGTACTTCAAAACAGAATCCTTCCAGTCCTCTT
CAGCGTTTAATTCCAGGCTCAAACTTGGACAGTGAACCCAGAATTCAAACAGATATACTAAAGCAGGCTACCAAGGACAGAGTCAGTGATTTCCATAAAT
TGAAGCAAAGCCGATTCTTTGATGAAAATGAATCCCCTGTTGATCCGCAGCATGGCTCTAAACTGGCGGATTATAATGGGGATGATGGTAACGTAGGTGA
GTATGAGGCAGACAAGCAGGCTGAGCTGGCTTACAATGAGGAAGAAGATGGTGATGGTGGAGAGGAAGACGTCCAAGATGATGAAGAACGAGAGCTTCAA
ATGGATCCTGCAGACTATGGAAAGCAACATTTCAATGATGTCCTTTAAGTCCTAAAGGAATGCTTCAGAAAACCTAAAGTGCTGTAAAATGAAATCATTC
TACTTTGTCCTTTCTGACTTTTGTTGTAAAGACGAATTGTATCAGTTGTAAAGATACATTGAGATAGAATTAAGGAAAAACTTTAATGAAGGAATGTACC
CATGTACATATGTGAACTTTTTCATATTGTATTATCAAGGTATAGACTTTTTTGGTTATGATACAGTTAAGCCAAAAACAGCTAATCTTTGCATCTAAAG
CAAACTAATGTATATTTCACATTTTATTGAGCCGACTTATTTCCACAAATAGATAAACAGGACAAAATAGTTGTACAGGTTATATGTGGCATAGCATAAC
CACAGTAAGAACAGAACAGATATTCAGCAGAAAACTTTTTATACTCTAATTCTTTTTTTTTTTTTTTTTGAGACAGAGTTTTAGTCTTGTTTCCCAGGCT
GGAGTGCAATGGCACAATCTTGGCTCACTGCAACCTCCGCCTCCTGGGTTCAGGCAATTTTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCACC
CACCACCATGCCCAGCTAATTTTTGTATTTTTAATAGAGAGCTAATAATTGTATATTTAATAAAGACGGGTTTCACCATGTTGGCCAGGCTGGTCTTGAA
CTCCTGACCTCAGGTGATCCTCCTGCATTGGCCTCCCAAAGTGCTGGAATTCCAGGCATGAGCCACTGCGCCCAGTCTACACACTAATTCTTGTTAGCCC
AACAGCTGTTCTGTTCTATCTACCCCTCATTTCACGCTCAAGGAGTCATACCTAGAATAGTTACACACAAGAGGGAAACTGGAAGCCAAACACTGTACAG
TATTGTGTAGAAAGTCACCTCCCTACTCCTTTTATTTTACATGAGTGCTGATGTGTTTTGGCAGATGAGCTTTCAGCTGAGGCCTGATGGAAATTGAGAT
AACCTGCAAAGACATAACAGTATTTATGAGTTATATCTTAGTTCTTGAAATTGTGGAATGCATGATTGACAATATATTTTTAATTTTTATTTTTTCAAGT
AATACCAGTACTGTTTAACTATAGCCAGAACTGGCTAAAATTTTTATATTTTCAGAGTTGAAGTTGGTGAAGACATTCATGATTTAAACACCAGATCCTG
AAAGGGGTTAAATCTACTTTGAAATGAATCTGCAATCAGTATTTCAAAGCTTTTCTGGTAATTTTAGTGATCTTATTTGATTAGACTTTTTCAGAAGTAC
TAAATAAGGAATTTTAACAGGTTTTTATTAATGCACAGATAAATAGAAGTACAGTGAGGTCTATAGCCATTTTATTAAAATAGCTTAAAAGTTTGTAAAA
AAATGAATCTTTGTAATTACTTAATATGTTAGTTAAGAACCCGTCAAGCTTATATTTGCTAGACTTACAAATTATTTTAAATGCATTTATCTTTTTTGAC
ACTATTCAGTGGAATGTGTAAGCTAGCTAATTCTTGTTTTCTGATTTAAAGCACTTTTAAATCTTATCCTGCCCCCTAAAAACAAAAGGTTTTGATCACA
AGGGGAAATTTAAGATTGTTAACCCTGTTTTTCAGAAGGGCTACTGTTAATTGCACATAAACATGAAATGTGTTTTCCCCTGTGTACTAACACATTCTAG
GCAAAATTCAAACTTATAGTGGTAAAGAAACAGGTTGTTCACTTGCTGAGGTGCAAAAATTCTTAAGACTTCTGTTTGAAATTGCTCAATGACTAGGAAA
AGATGTAGTAGTTTACTAAAATTGTTTTTCTACCATATCAAATTAAACAATTCATGCCTTTATAGGGTCAGGCCTACAATGAATAGGTATGGTGGTTTCA
CAGAATTTTAAAATAGAGTTAAAGGGAAGTGATGTACATTTCGGGGGCATTAGGGTAGGGAGATGAATCAAAAAATACCCCTAGTAATGCTTTATATTTT
AATACTGCAAAAGCTTTACAAATGGAAACCATGCAATTACCTGCCTTAGTTCTTTTGTCATAAAAACAATCACTTGGTTGGTTGTATTGTAGCTATTACT
TATACAGCAACATTTCTTCAATTAGCAGTCTAGACATTTTATAAACAGAAATCTTGGACCAATTGATAATATTTCTGACTGTATTAATATTTTAGTGCTA
TAAAATACTATGTGAATCTCTTAAAAATCTGACATTTTACAGTCTGTATTAGACATACTGTTTTTATAATGTTTTACTTCTGCCTTAAGATTTAGGTTTT
TTAAATGTATTTTTGCCCTGAATTAAGTGTTAATTTGATGGAAACTCTGCTTTTAAAATCATCATTTACTGGGTTCTAATAAATTAAAAATTAAACTTGT
TTC
  • CASC4.F1 (forward primer)
  • NM_177974-R1 (reverse primer)
  • Single exon skipping

Frame 0

TSAPFRFFSPPPNREVLGLGDAGSWVLPVPLGR
CRVGFKASGTRFGLI*GGGRGGTCGLRPRPLLRL
AADR*ARLLPRPALGPCPPGNSSRGLGFCLQVS
AARPLGYSPIWPHGGFRGQPAGWPPALSRAGGA
AGGDRRPRLQLLEHLLPPRPASGGGGRAAGPGPA
HRSGPRAAGKAQFGPLAVGGHAQETDRPEGGRL
RPPQQPAAGQRGPREEMRG*QG*TTEQHIVSDG
RHTSFKGATC*ASSGISSTRRPASGL*EEQYLPC
EEVRI*KFSVWTADEGIESTA*RKY*KVSRPVF
RGTKARDPKDSIK*WKGIGYKQSSST*KYSKSS
*ECCR*E*RTLKQSYSTWERTNQKRW*CRDAWNR
RE*PSKS**SSPCFKEASYFSFST*KSSSNLPS
SNWTTSLPKYASRFTHKPQWKPRYFKTESFQSS
SAFNSRLKLGQ*TQNSNRYTKAGYQGQSQ*FP*I
EAKPIL**K*IPC*SAAWL*TGGL*WG*W*RR*
V*GRQAG*AGLQ*GRRW*WWRGRRPR**RTRAS
NGSCRLWKATFQ*CPLSPKGMLQKT*SAVK*NHS
TLSFLTFVVKTNCISCKDTLR*N*GKTLMKECT
HVHM*TFSYCIIKV*TFLVMIQLSQKQLIFASK
AN*CIFHILLSRLISTNR*TGQNSCTGYMWHSIT
TVRTEQIFSRKLFIL*FFFFFFLRQSFSLVSQA
GVQWHNLGSLQPPPPGFRQFSCLSLPSSWDYRH
PPPCPANFCIFNRELIIVYLIKTGFTMLARLVLN
S*PQVILLHWPPKVLEFQA*ATAPSLHTNSC*P
NSCSVLSTPHFTLKESYLE*LHTRGKLEAKHCT
VLCRKSPPYSFYFT*VLMCFGR*AFS*GLMEIEI
TCKDITVFMSYILVLEIVECMIDNIFLIFIFSS
NTSTV*L*PELAKIFIFSELKLVKTFMI*TPDP
ERG*IYFEMNLQSVFQSFSGNFSDLI*LDFFRST
K*GILTGFY*CTDK*KYSEVYSHFIKIA*KFVK
K*IFVIT*YVS*EPVKLIFARLTNYFKCIYLF*
HYSVECVS*LILVF*FKALLNLILPPKNKRF*SQ
GEI*DC*PCFSEGLLLIAHKHEMCFPLCTNTF*
AKFKLIVVKKQVVHLLRCKNS*DFCLKLLND*E
KM**FTKIVFLPYQIKQFMPL*GQAYNE*VWWFH
RILK*S*REVMYISGALG*GDESKNTPSNALYF
NTAKALQMETMQLPALVLLS*KQSLGWLYCSYY
LYSNISSISSLDIL*TEILDQLIIFLTVLIF*CY
KILCESLKNLTFYSLY*TYCFYNVLLLP*DLGF
LNVFLP*IKC*FDGNSAFKIIIYWVLIN*KLNL
F

Frame 1

HPPLSGFFPRLPTVRCWVWGTLAAGFSRFPWAG
AGSGSKPPERVLA*FEEGGGEGPAACGPAPFSG
SQPTGKPASSLGRPWGRVRRATPAEAWASACRCL
RRGP*GTARFGPMVGFGANRRAGRLPSLVLVVL
LVVIVVLAFNYWSISSRHVLLQEEVAELQGQVQ
RTEVARGRLEKRNSDLLLLVDTHKKQIDQKEADY
GRLSSRLQAREGLGKRCEDDKVKLQNNISYQMA
DIHHLKEQLAELRQEFLRQEDQLQDYRKNNTYL
VKRLEYESFQCGQQMKELRAQHEENIKKLADQFL
EEQKQETQKIQSNDGKELDINNQVVPKNIPKVA
ENVADKNEEPSSNHIPHGKEQIKRGGDAGMPGI
EENDLAKVDDLPPALRKPPISVSQHESHQAISHL
PTGQPLSPNMPPDSHINHNGNPGTSKQNPSSPL
QRLIPGSNLDSEPRIQTDILKQATKDRVSDFHK
LKQSRFFDENESPVDPQHGSKLADYNGDDGNVGE
YEADKQAELAYNEEEDGDGGEEDVQDDEERELQ
MDPADYGKQHFNDVL*VLKECFRKPKVL*NEII
LLCPF*LLL*RRIVSVVKIH*DRIKEKL**RNVP
MYICELFHIVLSRYRLFWL*YS*AKNS*SLHLK
QTNVYFTFY*ADLFPQIDKQDKIVVQVICGIA*
PQ*EQNRYSAENFLYSNSFFFFF*DRVLVLFPRL
ECNGTILAHCNLRLLGSGNFPASASQVAGITGT
HHHAQLIFVFLIES**LYI**RRVSPCWPGWS*
TPDLR*SSCIGLPKCWNSRHEPLRPVYTLILVSP
TAVLFYLPLISRSRSHT*NSYTQEGNWKPNTVQ
YCVESHLPTPFILHEC*CVLADELSAEA*WKLR
*PAKT*QYL*VIS*FLKLWNA*LTIYF*FLFFQV
IPVLFNYSQNWLKFLYFQS*SW*RHS*FKHQIL
KGVKSTLK*ICNQYFKAFLVILVILFD*TFSEV
LNKEF*QVFINAQINRSTVRSIAILLK*LKSL*K
NESL*LLNMLVKNPSSLYLLDLQIILNAFIFFD
TIQWNV*AS*FLFSDLKHF*ILSCPLKTKGFDH
KGKFKIVNPVFQKGYC*LHINMKCVFPCVLTHSR
QNSNL*W*RNRLFTC*GAKILKTSV*NCSMTRK
RCSSLLKLFFYHIKLNNSCLYRVRPTMNRYGGF
TEF*NRVKGK*CTFRGH*GREMNQKIPLVMLYIL
ILQKLYKWKPCNYLP*FFCHKNNHLVGCIVAIT
YTATFLQLAV*TFYKQKSWTN**YF*LY*YFSA
IKYYVNLLKI*HFTVCIRHTVFIMFYFCLKI*VF
*MYFCPELSVNLMETLLLKSSFTGF**IKN*TC
F

Frame 2

IRPFPVFFPASQP*GVGFGGRWQLGSPGSLGQV
QGRVQSLRNAFWPDLRRGAGRDLRLAAPPPSPA
RSRPVSPPPPSAGPGAVSAGQLQPRPGLLPAGV
CGEAPRVQPDLAPWWVSGPTGGLAACPLSCWWCC
WW*SSSSPSTTGASPPATSCFRRRWPSCRARSS
APKWPAGGWKSAIRTSCCWWTRTRNRSTRRRPT
TAASAAGCRPERASGRDARMTRLNYRTTYRIRWQ
TYII*RSNLLSFVRNFFDKKTSFRTIGRTILTL
*RG*NMKVFSVDSR*RN*EHSMKKILKS*QTSF
*RNKSKRPKRFNQMMERNWI*TIK*YLKIFQK*L
RMLQIRMKNPQAIIFHMGKNKSKEVVMQGCLE*
KRMT*QKLMIFPLL*GSLLFQFLNMKVIKQSPI
FQLDNLSPQICLQIHT*TTMETPVLQNRILPVLF
SV*FQAQTWTVNPEFKQIY*SRLPRTESVISIN
*SKADSLMKMNPLLIRSMALNWRIIMGMMVT*V
SMRQTSRLSWLTMRKKMVMVERKTSKMMKNESFK
WILQTMESNISMMSFKS*RNASENLKCCKMKSF
YFVLSDFCCKDELYQL*RYIEIELRKNFNEGMY
PCTYVNFFILYYQGIDFFGYDTVKPKTANLCI*S
KLMYISHFIEPTYFHK*INRTK*LYRLYVA*HN
HSKNRTDIQQKTFYTLILFFFFFETEF*SCFPG
WSAMAQSWLTATSASWVQAIFLPQPPK*LGLQAP
TTMPS*FLYF**RANNCIFNKDGFHHVGQAGLE
LLTSGDPPALASQSAGIPGMSHCAQSTH*FLLA
QQLFCSIYPSFHAQGVIPRIVTHKRETGSQTLYS
IV*KVTSLLLLFYMSADVFWQMSFQLRPDGN*D
NLQRHNSIYELYLSS*NCGMHD*QYIFNFYFFK
*YQYCLTIARTG*NFYIFRVEVGEDIHDLNTRS*
KGLNLL*NESAISISKLFW*F**SYLIRLFQKY
*IRNFNRFLLMHR*IEVQ*GL*PFY*NSLKVCK
KMNLCNYLIC*LRTRQAYIC*TYKLF*MHLSFLT
LFSGMCKLANSCFLI*STFKSYPAP*KQKVLIT
RGNLRLLTLFFRRATVNCT*T*NVFSPVY*HIL
GKIQTYSGKETGCSLAEVQKFLRLLFEIAQ*LGK
DVVVY*NCFSTISN*TIHAFIGSGLQ*IGMVVS
QNFKIELKGSDVHFGGIRVGR*IKKYP**CFIF
*YCKSFTNGNHAITCLSSFVIKTITWLVVL*LLL
IQQHFFN*QSRHFINRNLGPIDNISDCINILVL
*NTM*IS*KSDILQSVLDILFL*CFTSALRFRF
FKCIFALN*VLI*WKLCF*NHHLLGSNKLKIKLV

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