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Sequences

Sequence ECT2.iAug05

ATTTTCCTCTTCCAGTCCTTAAAGCAGCTGATTTAGAAGAATACAAATCATGGCTGAAAATAGTGTATTAACATCCACTACTGGGAGGACTAGCTTGGCA GACTCTTCCATTTTTGATTCTAAAGTTACTGAGATTTCCAAGGAAAACTTACTTATTGGATCTACTTCATATGTAGAAGAAGAGATGCCTCAGATTGAAA CAAGAGTGATATTGGTTCAAGAAGCTGGAAAACAAGAAGAACTTATAAAAGCCTTAAAGGACATTAAAGTGGGCTTTGTAAAGATGGAGTCAGTGGAAGA ATTTGAAGGTTTGGATTCTCCGGAATTTGAAAATGTATTTGTAGTCACGGACTTTCAGGATTCTGTCTTTAATGACCTCTACAAGGCTGATTGTAGAGTT ATTGGACCACCAGTTGTATTAAATTGT

ECT2.F12 (forward primer)
ECT2.R11 (reverse primer)
Single exon skipping

Frame 0

IFLFQSLKQLI*KNTNHG*K*CINIHYWED*LG RLFHF*F*SY*DFQGKLTYWIYFICRRRDASD*N KSDIGSRSWKTRRTYKSLKGH*SGLCKDGVSGR I*RFGFSGI*KCICSHGLSGFCL**PLQG*L*S YWTTSCIKL

Frame 1

FSSSSP*SS*FRRIQIMAENSVLTSTTGRTSLA DSSIFDSKVTEISKENLLIGSTSYVEEEMPQIE TRVILVQEAGKQEELIKALKDIKVGFVKMESVEE FEGLDSPEFENVFVVTDFQDSVFNDLYKADCRV IGPPVVLNC

Frame 2

FPLPVLKAADLEEYKSWLKIVY*HPLLGGLAWQ TLPFLILKLLRFPRKTYLLDLLHM*KKRCLRLK QE*YWFKKLENKKNL*KP*RTLKWAL*RWSQWK NLKVWILRNLKMYL*SRTFRILSLMTSTRLIVEL LDHQLY*I

Sequence ECT2.bAug05

AGGTTGACATCACGCGCCAATCGGCATGGCTCTTAGAGAGAGCAGCTTAGTTTTTGAATCGGTTGTGGCGGCCGCCGGCGAGGAATGGCGGTATTTGTGA GAGGAGTCGGCGTTTGAAGAGGTGGAACTCCTAGGGCTTTTTTGAGAGTGACGGAGTCTACCTCTTGTTACCTAGACTGGAGTGCAGTGGCACGATCTCG GCTCACTGCAACCTCTGCCTCCCGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCTGCCACCAAGCCCAGCTAATTT TTGTATTTTTAGTAGAGACGGGGTTTCATTGTGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCGTGATCCGCCCGCCTTGGCCTCCCAAAGTGCTAGGA TTACAAGTGTGAGCCACCGCGTCCGGCCTTTCAAATGGTATTTTTGATTTTCCTCTTCCAGTCCTTAAAGCAGCTGATTTAGAAGAATACAAATCATGGC TGAAAATAGTGTATTAACATCCACTACTGGGAGGACTAGCTTGGCAGACTCTTCCATTTTTGATTCTAAAGTTACTGAGATTTCCAAGGAAAACTTACTT ATTGGATCTACTTCATATGTAGAAGAGATGCCTCAGATTGAAACAAGAGTGATATTGGTTCAAGAAGCTGGAAAACAAGAAGAACTTATAAAAGCCTTAA AGGACATTAAAGTGGGCTTTGTAAAGATGGAGTCAGTGGAAGAATTTGAAGGTTTGGATTCTCCGGAATTTGAAAATGTATTTGTAGTCACGGACTTTCA GGATTCTGTCTTTAATGACCTCTACAAGGCTGATTGTAGAGTTATTGGACCACCAGTTGTATTAAATTGTTCACAAAAAGGAGAGCCTTTGCCATTTTCA TGTCGCCCGTTGTATTGTACAAGTATGATGAATCTAGTACTATGCTTTACTGGATTTAGGAAAAAAGAAGAACTAGTCAGGTTGGTGACATTGGTCCATC ACATGGGTGGAGTTATTCGAAAAGACTTTAATTCAAAAGTTACACATTTGGTGGCAAATTGTACACAAGGAGAAAAATTCAGGGTTGCTGTGAGTCTAGG TACTCCAATTATGAAGCCAGAATGGATTTATAAAGCTTGGGAAAGGCGGAATGAACAGGATTTCTATGCAGCAGTTGATGACTTTAGAAATGAATTTAAA GTTCCTCCATTTCAAGATTGTATTTTAAGTTTCCTGGGATTTTCAGATGAAGAGAAAACCAATATGGAAGAAATGACTGAAATGCAAGGAGGTAAATATT TACCGCTTGGAGATGAAAGATGCACTCACCTTGTAGTTGAAGAGAATATAGTAAAAGATCTTCCCTTTGAACCTTCAAAGAAACTTTATGTTGTCAAGCA AGAGTGGTTCTGGGGAAGCATTCAAATGGATGCCCGAGCTGGAGAAACTATGTATTTATATGAAAAGGCAAATACTCCTGAGCTCAAGAAATCAGTGTCA ATGCTTTCTCTAAATACCCCTAACAGCAATCGCAAACGACGTCGTTTAAAAGAAACACTTGCTCAGCTTTCAAGAGAGACAGACGTGTCACCATTTCCAC CCCGTAAGCGCCCATCAGCTGAGCATTCCCTTTCCATAGGGTCACTCCTAGATATCTCCAACACACCAGAGTCTAGCATTAACTATGGAGACACCCCAAA GTCTTGTACTAAGTCTTCTAAAAGCTCCACTCCAGTTCCTTCAAAGCAGTCAGCAAGGTGGCAAGTTGCAAAAGAGCTTTATCAAACTGAAAGTAATTAT GTTAATATATTGGCAACAATTATTCAGTTATTTCAAGTACCATTGGAAGAGGAAGGACAACGTGGTGGACCTATCCTTGCACCAGAGGAGATTAAGACTA TTTTTGGTAGCATCCCAGATATCTTTGATGTACACACTAAGATAAAGGATGATCTTGAAGACCTTATAGTTAATTGGGATGAGAGCAAAAGCATTGGTGA CATTTTTCTGAAATATTCAAAAGATTTGGTAAAAACCTACCCTCCCTTTGTAAACTTCTTTGAAATGAGCAAGGAAACAATTATTAAATGTGAAAAACAG AAACCAAGATTTCATGCTTTTCTCAAGATAAACCAAGCAAAACCAGAATGTGGACGGCAGAGCCTTGTTGAACTTCTTATCCGACCAGTACAGAGGTTAC CCAGTGTTGCATTACTTTTAAATGATCTTAAGAAGCATACAGCTGATGAAAATCCAGACAAAAGCACTTTAGAAAAAGCTATTGGATCACTGAAGGAAGT AATGACGCATATTAATGAGGATAAGAGAAAAACAGAAGCTCAAAAGCAAATTTTTGATGTTGTTTATGAAGTAGATGGATGCCCAGCTAATCTTTTATCT TCTCACCGAAGCTTAGTACAGCGGGTTGAAACAATTTCTCTAGGTGAGCACCCCTGTGACAGAGGAGAACAAGTAACTCTCTTCCTCTTCAATGATTGCC TAGAGATAGCAAGAAAACGGCACAAGGTTATTGGCACTTTTAGGAGTCCTCATGGCCAAACCCGACCCCCAGCTTCTCTTAAGCATATTCACCTAATGCC TCTTTCTCAGATTAAGAAGGTATTGGACATAAGAGAGACAGAAGATTGCCATAATGCTTTTGCCTTGCTTGTGAGGCCACCAACAGAGCAGGCAAATGTG CTACTCAGTTTCCAGATGACATCAGATGAACTTCCAAAAGAAAACTGGCTAAAGATGCTGTGTCGACATGTAGCTAACACCATTTGTAAAGCAGATGCTG AGAATCTTATTTATACTGCTGATCCAGAATCCTTTGAAGTAAATACAAAAGATATGGACAGTACATTGAGTAGAGCATCAAGAGCAATAAAAAAGACTTC AAAAAAGGTTACAAGAGCATTCTCTTTCTCCAAAACTCCAAAAAGAGCTCTTCGAAGGGCTCTTATGACATCCCACGGCTCAGTGGAGGGAAGAAGTCCT TCCAGCAATGATAAGCATGTAATGAGTCGTCTTTCTAGCACATCATCATTAGCAGGTATCCCTTCTCCCTCCCTTGTCAGCCTTCCTTCCTTCTTTGAAA GGAGAAGTCATACGTTAAGTAGATCTACAACTCATTTGATATGAAGCGTTACCAAAATCTTAAATTATAGAAATGTATAGACACCTCATACTCAAATAAG AAACTGACTTAAATGGTACTTGTAATTAGCACTTGGTGAAAGCTGGAAGGAAGATAAATAACACTAAACTATGCTATTTGATTTTTCTTCTTGAAAGAGT AAGGTTTACCTGTTACATTTTCAAGTTAATTCATGTAAAAAATGATAGTGATTTTGATGTAATTTATCTCTTGTTTGAATCTGTCATTCAAAGGCCAATA ATTTAAGTTGCTATCAGCTGATATTAGTAGCTTTGCAACCCTGATAGAGTAAATAAATTTTATGGGCGGGTGCCAAATACTGCTGTGAATCTATTTGTAT AGTATCCATGAATGAATTTATGGAAATAGATATTTGTGCAGCTCAATTTATGCAGAGATTAAATGACATCATAATACTGGATGAAAACTTGCATAGAATT CTGATTAAATAGTGGGTCTGTTTCACATGTGCAGTTTGAAGTATTTAAATAACCACTCCTTTCACAGTTTATTTTCTTCTCAAGCGTTTTCAAGATCTAG CATGTGGATTTTAAAAGATTTGCCCTCATTAACAAGAATAACATTTAAAGGAGATTGTTTCAAAATATTTTTGCAAATTGAGATAAGGACAGAAAGATTG AGAAACATTGTATATTTTGCAAAAACAAGATGTTTGTAGCTGTTTCAGAGAGAGTACGGTATATTTATGGTAATTTTATCCACTAGCAAATCTTGATTTA GTTTGATAGTGTGTGGAATTTTATTTTGAAGGATAAGACCATGGGAAAATTGTGGTAAAGACTGTTTGTACCCTTCATGAAATAATTCTGAAGTTGCCAT CAGTTTTACTAATCTTCTGTGAAATGCATAGATATGCGCATGTTCAACTTTTTATTGTGGTCTTATAATTAAATGTAAAATTGAAAATTCATTTGCTGTT TCAAAGTGTGATATCTTTCACAATAGCCTTTTTATAGTCAGTAATTCAGAATAATCAAGTTCATATGGATAAATGCATTTTTATTTCCTATTTCTTTAGG GAGTGCTACAAATGTTTGTCACTTAAATTTCAAGTTTCTGTTTTAATAGTTAACTGACTATAGATTGTTTTCTATGCCATGTATGTGCCACTTCTGAGAG TAGTAAATGACTCTTTGCTACATTTTAAAAGCAATTGTATTAGTAAGAACTTTGTAAATAAATACCTAAAACCCAAGTGTA

ECT2.F12 (forward primer)
ECT2.R11 (reverse primer)
Single exon skipping

Frame 0

RLTSRANRHGS*REQLSF*IGCGGRRRGMAVFV RGVGV*RGGTPRAFLRVTESTSCYLDWSAVARSR LTATSASRVQAILLPQPPE*LGLQVPATKPS*F LYF**RRGFIVLARLVSNS*PRDPPALASQSAR ITSVSHRVRPFKWYF*FSSSSP*SS*FRRIQIMA ENSVLTSTTGRTSLADSSIFDSKVTEISKENLL IGSTSYVEEMPQIETRVILVQEAGKQEELIKAL KDIKVGFVKMESVEEFEGLDSPEFENVFVVTDFQ DSVFNDLYKADCRVIGPPVVLNCSQKGEPLPFS CRPLYCTSMMNLVLCFTGFRKKEELVRLVTLVH HMGGVIRKDFNSKVTHLVANCTQGEKFRVAVSLG TPIMKPEWIYKAWERRNEQDFYAAVDDFRNEFK VPPFQDCILSFLGFSDEEKTNMEEMTEMQGGKY LPLGDERCTHLVVEENIVKDLPFEPSKKLYVVKQ EWFWGSIQMDARAGETMYLYEKANTPELKKSVS MLSLNTPNSNRKRRRLKETLAQLSRETDVSPFP PRKRPSAEHSLSIGSLLDISNTPESSINYGDTPK SCTKSSKSSTPVPSKQSARWQVAKELYQTESNY VNILATIIQLFQVPLEEEGQRGGPILAPEEIKT IFGSIPDIFDVHTKIKDDLEDLIVNWDESKSIGD IFLKYSKDLVKTYPPFVNFFEMSKETIIKCEKQ KPRFHAFLKINQAKPECGRQSLVELLIRPVQRL PSVALLLNDLKKHTADENPDKSTLEKAIGSLKEV MTHINEDKRKTEAQKQIFDVVYEVDGCPANLLS SHRSLVQRVETISLGEHPCDRGEQVTLFLFNDC LEIARKRHKVIGTFRSPHGQTRPPASLKHIHLMP LSQIKKVLDIRETEDCHNAFALLVRPPTEQANV LLSFQMTSDELPKENWLKMLCRHVANTICKADA ENLIYTADPESFEVNTKDMDSTLSRASRAIKKTS KKVTRAFSFSKTPKRALRRALMTSHGSVEGRSP SSNDKHVMSRLSSTSSLAGIPSPSLVSLPSFFE RRSHTLSRSTTHLI*SVTKILNYRNV*TPHTQIR N*LKWYL*LALGESWKEDK*H*TMLFDFSS*KS KVYLLHFQVNSCKK***F*CNLSLV*ICHSKAN NLSCYQLILVALQP**SK*ILWAGAKYCCESICI VSMNEFMEIDICAAQFMQRLNDIIILDENLHRI LIK*WVCFTCAV*SI*ITTPFTVYFLLKRFQDL ACGF*KICPH*QE*HLKEIVSKYFCKLR*GQKD* ETLYILQKQDVCSCFRESTVYLW*FYPLANLDL V**CVEFYFEG*DHGKIVVKTVCTLHEIILKLP SVLLIFCEMHRYAHVQLFIVVL*LNVKLKIHLLF QSVISFTIAFL*SVIQNNQVHMDKCIFISYFFR ECYKCLSLKFQVSVLIVN*L*IVFYAMYVPLLR VVNDSLLHFKSNCISKNFVNKYLKPKC

Frame 1

G*HHAPIGMALRESSLVFESVVAAAGEEWRYL* EESAFEEVELLGLF*E*RSLPLVT*TGVQWHDL GSLQPLPPGFKRFSCLSLLSSWDYRCLPPSPANF CIFSRDGVSLCWPGWSRTPDLVIRPPWPPKVLG LQV*ATASGLSNGIFDFPLPVLKAADLEEYKSW LKIVY*HPLLGGLAWQTLPFLILKLLRFPRKTYL LDLLHM*KRCLRLKQE*YWFKKLENKKNL*KP* RTLKWAL*RWSQWKNLKVWILRNLKMYL*SRTF RILSLMTSTRLIVELLDHQLY*IVHKKESLCHFH VARCIVQV**I*YYALLDLGKKKN*SGW*HWSI TWVELFEKTLIQKLHIWWQIVHKEKNSGLL*V* VLQL*SQNGFIKLGKGGMNRISMQQLMTLEMNLK FLHFKIVF*VSWDFQMKRKPIWKK*LKCKEVNI YRLEMKDALTL*LKRI**KIFPLNLQRNFMLSS KSGSGEAFKWMPELEKLCIYMKRQILLSSRNQCQ CFL*IPLTAIANDVV*KKHLLSFQERQTCHHFH PVSAHQLSIPFP*GHS*ISPTHQSLALTMETPQ SLVLSLLKAPLQFLQSSQQGGKLQKSFIKLKVIM LIYWQQLFSYFKYHWKRKDNVVDLSLHQRRLRL FLVASQISLMYTLR*RMILKTL*LIGMRAKALV TFF*NIQKIW*KPTLPL*TSLK*ARKQLLNVKNR NQDFMLFSR*TKQNQNVDGRALLNFLSDQYRGY PVLHYF*MILRSIQLMKIQTKAL*KKLLDH*RK **RILMRIREKQKLKSKFLMLFMK*MDAQLIFYL LTEA*YSGLKQFL*VSTPVTEENK*LSSSSMIA *R*QENGTRLLALLGVLMAKPDPQLLLSIFT*C LFLRLRRYWT*ERQKIAIMLLPCL*GHQQSRQMC YSVSR*HQMNFQKKTG*RCCVDM*LTPFVKQML RILFILLIQNPLK*IQKIWTVH*VEHQEQ*KRL QKRLQEHSLSPKLQKELFEGLL*HPTAQWREEVL PAMISM**VVFLAHHH*QVSLLPPLSAFLPSLK GEVIR*VDLQLI*YEALPKS*IIEMYRHLILK* ETDLNGTCN*HLVKAGRKINNTKLCYLIFLLERV RFTCYIFKLIHVKNDSDFDVIYLLFESVIQRPI I*VAIS*Y**LCNPDRVNKFYGRVPNTAVNLFV *YP*MNLWK*IFVQLNLCRD*MTS*YWMKTCIEF *LNSGSVSHVQFEVFK*PLLSQFIFFSSVFKI* HVDFKRFALINKNNI*RRLFQNIFAN*DKDRKI EKHCIFCKNKMFVAVSERVRYIYGNFIH*QILI* FDSVWNFILKDKTMGKLW*RLFVPFMK*F*SCH QFY*SSVKCIDMRMFNFLLWSYN*M*N*KFICC FKV*YLSQ*PFYSQ*FRIIKFIWINAFLFPISLG SATNVCHLNFKFLF**LTDYRLFSMPCMCHF*E **MTLCYILKAIVLVRTL*INT*NPSV

Frame 2

VDITRQSAWLLERAA*FLNRLWRPPARNGGICE RSRRLKRWNS*GFFESDGVYLLLPRLECSGTIS AHCNLCLPGSSDSPASAS*VAGITGACHQAQLI FVFLVETGFHCVGQAGLELLTS*SARLGLPKC*D YKCEPPRPAFQMVFLIFLFQSLKQLI*KNTNHG *K*CINIHYWED*LGRLFHF*F*SY*DFQGKLT YWIYFICRRDASD*NKSDIGSRSWKTRRTYKSLK GH*SGLCKDGVSGRI*RFGFSGI*KCICSHGLS GFCL**PLQG*L*SYWTTSCIKLFTKRRAFAIF MSPVVLYKYDESSTMLYWI*EKRRTSQVGDIGPS HGWSYSKRL*FKSYTFGGKLYTRRKIQGCCESR YSNYEARMDL*SLGKAE*TGFLCSS**L*K*I* SSSISRLYFKFPGIFR*RENQYGRND*NARR*IF TAWR*KMHSPCS*REYSKRSSL*TFKETLCCQA RVVLGKHSNGCPSWRNYVFI*KGKYS*AQEISV NAFSKYP*QQSQTTSFKRNTCSAFKRDRRVTIST P*APIS*AFPFHRVTPRYLQHTRV*H*LWRHPK VLY*VF*KLHSSSFKAVSKVASCKRALSN*K*L C*YIGNNYSVISSTIGRGRTTWWTYPCTRGD*DY FW*HPRYL*CTH*DKG*S*RPYS*LG*EQKHW* HFSEIFKRFGKNLPSLCKLL*NEQGNNY*M*KT ETKISCFSQDKPSKTRMWTAEPC*TSYPTSTEVT QCCITFK*S*EAYS**KSRQKHFRKSYWITEGS NDAY**G*EKNRSSKANF*CCL*SRWMPS*SFI FSPKLSTAG*NNFSR*APL*QRRTSNSLPLQ*LP RDSKKTAQGYWHF*ESSWPNPTPSFS*AYSPNA SFSD*EGIGHKRDRRLP*CFCLACEATNRAGKC ATQFPDDIR*TSKRKLAKDAVSTCS*HHL*SRC* ESYLYC*SRIL*SKYKRYGQYIE*SIKSNKKDF KKGYKSILFLQNSKKSSSKGSYDIPRLSGGKKS FQQ**ACNESSF*HIIISRYPFSLPCQPSFLL*K EKSYVK*IYNSFDMKRYQNLKL*KCIDTSYSNK KLT*MVLVISTW*KLEGR*ITLNYAI*FFFLKE *GLPVTFSS*FM*KMIVILM*FISCLNLSFKGQ* FKLLSADISSFATLIE*INFMGGCQILL*IYLY SIHE*IYGNRYLCSSIYAEIK*HHNTG*KLA*N SD*IVGLFHMCSLKYLNNHSFHSLFSSQAFSRSS MWILKDLPSLTRITFKGDCFKIFLQIEIRTERL RNIVYFAKTRCL*LFQREYGIFMVILSTSKS*F SLIVCGILF*RIRPWENCGKDCLYPS*NNSEVAI SFTNLL*NA*ICACSTFYCGLIIKCKIENSFAV SKCDIFHNSLFIVSNSE*SSSYG*MHFYFLFL* GVLQMFVT*ISSFCFNS*LTIDCFLCHVCATSES SK*LFATF*KQLY**ELCK*IPKTQV

Sequence ECT2.aAug05

AGGTTGACATCACGCGCCAATCGGCATGGCTCTTAGAGAGAGCAGCTTAGTTTTTGAATCGGTTGTGGCGGCCGCCGGCGAGGAATGGCGGTATTTGTGA GAGGAGTCGGCGTTTGAAGAGGTGGAACTCCTAGGGCTTTTTTGAGAGTGCTGATTTAGAAGAATACAAATCATGGCTGAAAATAGTGTATTAACATCCA CTACTGGGAGGACTAGCTTGGCAGACTCTTCCATTTTTGATTCTAAAGTTACTGAGATTTCCAAGGAAAACTTACTTATTGGATCTACTTCATATGTAGA AGAAGAGATGCCTCAGATTGAAACAAGAGTGATATTGGTTCAAGAAGCTGGAAAACAAGAAGAACTTATAAAAGCCTTAAAGGACATTAAAGTGGGCTTT GTAAAGATGGAGTCAGTGGAAGAATTTGAAGGTTTGGATTCTCCGGAATTTGAAAATGTATTTGTAGTCACGGACTTTCAGGATTCTGTCTTTAATGACC TCTACAAGGCTGATTGTAGAGTTATTGGACCACCAGTTGTATTAAATTGTTCACAAAAAGGAGAGCCTTTGCCATTTTCATGTCGCCCGTTGTATTGTAC AAGTATGATGAATCTAGTACTATGCTTTACTGGATTTAGGAAAAAAGAAGAACTAGTCAGGTTGGTGACATTGGTCCATCACATGGGTGGAGTTATTCGA AAAGACTTTAATTCAAAAGTTACACATTTGGTGGCAAATTGTACACAAGGAGAAAAATTCAGGGTTGCTGTGAGTCTAGGTACTCCAATTATGAAGCCAG AATGGATTTATAAAGCTTGGGAAAGGCGGAATGAACAGGATTTCTATGCAGCAGTTGATGACTTTAGAAATGAATTTAAAGTTCCTCCATTTCAAGATTG TATTTTAAGTTTCCTGGGATTTTCAGATGAAGAGAAAACCAATATGGAAGAAATGACTGAAATGCAAGGAGGTAAATATTTACCGCTTGGAGATGAAAGA TGCACTCACCTTGTAGTTGAAGAGAATATAGTAAAAGATCTTCCCTTTGAACCTTCAAAGAAACTTTATGTTGTCAAGCAAGAGTGGTTCTGGGGAAGCA TTCAAATGGATGCCCGAGCTGGAGAAACTATGTATTTATATGAAAAGGCAAATACTCCTGAGCTCAAGAAATCAGTGTCAATGCTTTCTCTAAATACCCC TAACAGCAATCGCAAACGACGTCGTTTAAAAGAAACACTTGCTCAGCTTTCAAGAGAGACAGACGTGTCACCATTTCCACCCCGTAAGCGCCCATCAGCT GAGCATTCCCTTTCCATAGGGTCACTCCTAGATATCTCCAACACACCAGAGTCTAGCATTAACTATGGAGACACCCCAAAGTCTTGTACTAAGTCTTCTA AAAGCTCCACTCCAGTTCCTTCAAAGCAGTCAGCAAGGTGGCAAGTTGCAAAAGAGCTTTATCAAACTGAAAGTAATTATGTTAATATATTGGCAACAAT TATTCAGTTATTTCAAGTACCATTGGAAGAGGAAGGACAACGTGGTGGACCTATCCTTGCACCAGAGGAGATTAAGACTATTTTTGGTAGCATCCCAGAT ATCTTTGATGTACACACTAAGATAAAGGATGATCTTGAAGACCTTATAGTTAATTGGGATGAGAGCAAAAGCATTGGTGACATTTTTCTGAAATATTCAA AAGATTTGGTAAAAACCTACCCTCCCTTTGTAAACTTCTTTGAAATGAGCAAGGAAACAATTATTAAATGTGAAAAACAGAAACCAAGATTTCATGCTTT TCTCAAGATAAACCAAGCAAAACCAGAATGTGGACGGCAGAGCCTTGTTGAACTTCTTATCCGACCAGTACAGAGGTTACCCAGTGTTGCATTACTTTTA AATGATCTTAAGAAGCATACAGCTGATGAAAATCCAGACAAAAGCACTTTAGAAAAAGCTATTGGATCACTGAAGGAAGTAATGACGCATATTAATGAGG ATAAGAGAAAAACAGAAGCTCAAAAGCAAATTTTTGATGTTGTTTATGAAGTAGATGGATGCCCAGCTAATCTTTTATCTTCTCACCGAAGCTTAGTACA GCGGGTTGAAACAATTTCTCTAGGTGAGCACCCCTGTGACAGAGGAGAACAAGTAACTCTCTTCCTCTTCAATGATTGCCTAGAGATAGCAAGAAAACGG CACAAGGTTATTGGCACTTTTAGGAGTCCTCATGGCCAAACCCGACCCCCAGCTTCTCTTAAGCATATTCACCTAATGCCTCTTTCTCAGATTAAGAAGG TATTGGACATAAGAGAGACAGAAGATTGCCATAATGCTTTTGCCTTGCTTGTGAGGCCACCAACAGAGCAGGCAAATGTGCTACTCAGTTTCCAGATGAC ATCAGATGAACTTCCAAAAGAAAACTGGCTAAAGATGCTGTGTCGACATGTAGCTAACACCATTTGTAAAGCAGATGCTGAGAATCTTATTTATACTGCT GATCCAGAATCCTTTGAAGTAAATACAAAAGATATGGACAGTACATTGAGTAGAGCATCAAGAGCAATAAAAAAGACTTCAAAAAAGGTTACAAGAGCAT TCTCTTTCTCCAAAACTCCAAAAAGAGCTCTTCGAAGGGCTCTTATGACATCCCACGGCTCAGTGGAGGGAAGAAGTCCTTCCAGCAATGATAAGCATGT AATGAGTCGTCTTTCTAGCACATCATCATTAGCAGGTATCCCTTCTCCCTCCCTTGTCAGCCTTCCTTCCTTCTTTGAAAGGAGAAGTCATACGTTAAGT AGATCTACAACTCATTTGATATGAAGCGTTACCAAAATCTTAAATTATAGAAATGTATAGACACCTCATACTCAAATAAGAAACTGACTTAAATGGTACT TGTAATTAGCACTTGGTGAAAGCTGGAAGGAAGATAAATAACACTAAACTATGCTATTTGATTTTTCTTCTTGAAAGAGTAAGGTTTACCTGTTACATTT TCAAGTTAATTCATGTAAAAAATGATAGTGATTTTGATGTAATTTATCTCTTGTTTGAATCTGTCATTCAAAGGCCAATAATTTAAGTTGCTATCAGCTG ATATTAGTAGCTTTGCAACCCTGATAGAGTAAATAAATTTTATGGGCGGGTGCCAAATACTGCTGTGAATCTATTTGTATAGTATCCATGAATGAATTTA TGGAAATAGATATTTGTGCAGCTCAATTTATGCAGAGATTAAATGACATCATAATACTGGATGAAAACTTGCATAGAATTCTGATTAAATAGTGGGTCTG TTTCACATGTGCAGTTTGAAGTATTTAAATAACCACTCCTTTCACAGTTTATTTTCTTCTCAAGCGTTTTCAAGATCTAGCATGTGGATTTTAAAAGATT TGCCCTCATTAACAAGAATAACATTTAAAGGAGATTGTTTCAAAATATTTTTGCAAATTGAGATAAGGACAGAAAGATTGAGAAACATTGTATATTTTGC AAAAACAAGATGTTTGTAGCTGTTTCAGAGAGAGTACGGTATATTTATGGTAATTTTATCCACTAGCAAATCTTGATTTAGTTTGATAGTGTGTGGAATT TTATTTTGAAGGATAAGACCATGGGAAAATTGTGGTAAAGACTGTTTGTACCCTTCATGAAATAATTCTGAAGTTGCCATCAGTTTTACTAATCTTCTGT GAAATGCATAGATATGCGCATGTTCAACTTTTTATTGTGGTCTTATAATTAAATGTAAAATTGAAAATTCATTTGCTGTTTCAAAGTGTGATATCTTTCA CAATAGCCTTTTTATAGTCAGTAATTCAGAATAATCAAGTTCATATGGATAAATGCATTTTTATTTCCTATTTCTTTAGGGAGTGCTACAAATGTTTGTC ACTTAAATTTCAAGTTTCTGTTTTAATAGTTAACTGACTATAGATTGTTTTCTATGCCATGTATGTGCCACTTCTGAGAGTAGTAAATGACTCTTTGCTA CATTTTAAAAGCAATTGTATTAGTAAGAACTTTGTAAATAAATACCTAAAACCCAAGTGTA

ECT2.F12 (forward primer)
ECT2.R11 (reverse primer)
Single exon skipping

Frame 0

RLTSRANRHGS*REQLSF*IGCGGRRRGMAVFV RGVGV*RGGTPRAFLRVLI*KNTNHG*K*CINIH YWED*LGRLFHF*F*SY*DFQGKLTYWIYFICR RRDASD*NKSDIGSRSWKTRRTYKSLKGH*SGL CKDGVSGRI*RFGFSGI*KCICSHGLSGFCL**P LQG*L*SYWTTSCIKLFTKRRAFAIFMSPVVLY KYDESSTMLYWI*EKRRTSQVGDIGPSHGWSYS KRL*FKSYTFGGKLYTRRKIQGCCESRYSNYEAR MDL*SLGKAE*TGFLCSS**L*K*I*SSSISRL YFKFPGIFR*RENQYGRND*NARR*IFTAWR*K MHSPCS*REYSKRSSL*TFKETLCCQARVVLGKH SNGCPSWRNYVFI*KGKYS*AQEISVNAFSKYP *QQSQTTSFKRNTCSAFKRDRRVTISTP*APIS *AFPFHRVTPRYLQHTRV*H*LWRHPKVLY*VF* KLHSSSFKAVSKVASCKRALSN*K*LC*YIGNN YSVISSTIGRGRTTWWTYPCTRGD*DYFW*HPR YL*CTH*DKG*S*RPYS*LG*EQKHW*HFSEIFK RFGKNLPSLCKLL*NEQGNNY*M*KTETKISCF SQDKPSKTRMWTAEPC*TSYPTSTEVTQCCITF K*S*EAYS**KSRQKHFRKSYWITEGSNDAY**G *EKNRSSKANF*CCL*SRWMPS*SFIFSPKLST AG*NNFSR*APL*QRRTSNSLPLQ*LPRDSKKT AQGYWHF*ESSWPNPTPSFS*AYSPNASFSD*EG IGHKRDRRLP*CFCLACEATNRAGKCATQFPDD IR*TSKRKLAKDAVSTCS*HHL*SRC*ESYLYC *SRIL*SKYKRYGQYIE*SIKSNKKDFKKGYKSI LFLQNSKKSSSKGSYDIPRLSGGKKSFQQ**AC NESSF*HIIISRYPFSLPCQPSFLL*KEKSYVK *IYNSFDMKRYQNLKL*KCIDTSYSNKKLT*MVL VISTW*KLEGR*ITLNYAI*FFFLKE*GLPVTF SS*FM*KMIVILM*FISCLNLSFKGQ*FKLLSA DISSFATLIE*INFMGGCQILL*IYLYSIHE*IY GNRYLCSSIYAEIK*HHNTG*KLA*NSD*IVGL FHMCSLKYLNNHSFHSLFSSQAFSRSSMWILKD LPSLTRITFKGDCFKIFLQIEIRTERLRNIVYFA KTRCL*LFQREYGIFMVILSTSKS*FSLIVCGI LF*RIRPWENCGKDCLYPS*NNSEVAISFTNLL *NA*ICACSTFYCGLIIKCKIENSFAVSKCDIFH NSLFIVSNSE*SSSYG*MHFYFLFL*GVLQMFV T*ISSFCFNS*LTIDCFLCHVCATSESSK*LFA TF*KQLY**ELCK*IPKTQV

Frame 1

G*HHAPIGMALRESSLVFESVVAAAGEEWRYL* EESAFEEVELLGLF*EC*FRRIQIMAENSVLTS TTGRTSLADSSIFDSKVTEISKENLLIGSTSYVE EEMPQIETRVILVQEAGKQEELIKALKDIKVGF VKMESVEEFEGLDSPEFENVFVVTDFQDSVFND LYKADCRVIGPPVVLNCSQKGEPLPFSCRPLYCT SMMNLVLCFTGFRKKEELVRLVTLVHHMGGVIR KDFNSKVTHLVANCTQGEKFRVAVSLGTPIMKP EWIYKAWERRNEQDFYAAVDDFRNEFKVPPFQDC ILSFLGFSDEEKTNMEEMTEMQGGKYLPLGDER CTHLVVEENIVKDLPFEPSKKLYVVKQEWFWGS IQMDARAGETMYLYEKANTPELKKSVSMLSLNTP NSNRKRRRLKETLAQLSRETDVSPFPPRKRPSA EHSLSIGSLLDISNTPESSINYGDTPKSCTKSS KSSTPVPSKQSARWQVAKELYQTESNYVNILATI IQLFQVPLEEEGQRGGPILAPEEIKTIFGSIPD IFDVHTKIKDDLEDLIVNWDESKSIGDIFLKYS KDLVKTYPPFVNFFEMSKETIIKCEKQKPRFHAF LKINQAKPECGRQSLVELLIRPVQRLPSVALLL NDLKKHTADENPDKSTLEKAIGSLKEVMTHINE DKRKTEAQKQIFDVVYEVDGCPANLLSSHRSLVQ RVETISLGEHPCDRGEQVTLFLFNDCLEIARKR HKVIGTFRSPHGQTRPPASLKHIHLMPLSQIKK VLDIRETEDCHNAFALLVRPPTEQANVLLSFQMT SDELPKENWLKMLCRHVANTICKADAENLIYTA DPESFEVNTKDMDSTLSRASRAIKKTSKKVTRA FSFSKTPKRALRRALMTSHGSVEGRSPSSNDKHV MSRLSSTSSLAGIPSPSLVSLPSFFERRSHTLS RSTTHLI*SVTKILNYRNV*TPHTQIRN*LKWY L*LALGESWKEDK*H*TMLFDFSS*KSKVYLLHF QVNSCKK***F*CNLSLV*ICHSKANNLSCYQL ILVALQP**SK*ILWAGAKYCCESICIVSMNEF MEIDICAAQFMQRLNDIIILDENLHRILIK*WVC FTCAV*SI*ITTPFTVYFLLKRFQDLACGF*KI CPH*QE*HLKEIVSKYFCKLR*GQKD*ETLYIL QKQDVCSCFRESTVYLW*FYPLANLDLV**CVEF YFEG*DHGKIVVKTVCTLHEIILKLPSVLLIFC EMHRYAHVQLFIVVL*LNVKLKIHLLFQSVISF TIAFL*SVIQNNQVHMDKCIFISYFFRECYKCLS LKFQVSVLIVN*L*IVFYAMYVPLLRVVNDSLL HFKSNCISKNFVNKYLKPKC

Frame 2

VDITRQSAWLLERAA*FLNRLWRPPARNGGICE RSRRLKRWNS*GFFESADLEEYKSWLKIVY*HP LLGGLAWQTLPFLILKLLRFPRKTYLLDLLHM* KKRCLRLKQE*YWFKKLENKKNL*KP*RTLKWAL *RWSQWKNLKVWILRNLKMYL*SRTFRILSLMT STRLIVELLDHQLY*IVHKKESLCHFHVARCIV QV**I*YYALLDLGKKKN*SGW*HWSITWVELFE KTLIQKLHIWWQIVHKEKNSGLL*V*VLQL*SQ NGFIKLGKGGMNRISMQQLMTLEMNLKFLHFKI VF*VSWDFQMKRKPIWKK*LKCKEVNIYRLEMKD ALTL*LKRI**KIFPLNLQRNFMLSSKSGSGEA FKWMPELEKLCIYMKRQILLSSRNQCQCFL*IP LTAIANDVV*KKHLLSFQERQTCHHFHPVSAHQL SIPFP*GHS*ISPTHQSLALTMETPQSLVLSLL KAPLQFLQSSQQGGKLQKSFIKLKVIMLIYWQQ LFSYFKYHWKRKDNVVDLSLHQRRLRLFLVASQI SLMYTLR*RMILKTL*LIGMRAKALVTFF*NIQ KIW*KPTLPL*TSLK*ARKQLLNVKNRNQDFML FSR*TKQNQNVDGRALLNFLSDQYRGYPVLHYF* MILRSIQLMKIQTKAL*KKLLDH*RK**RILMR IREKQKLKSKFLMLFMK*MDAQLIFYLLTEA*Y SGLKQFL*VSTPVTEENK*LSSSSMIA*R*QENG TRLLALLGVLMAKPDPQLLLSIFT*CLFLRLRR YWT*ERQKIAIMLLPCL*GHQQSRQMCYSVSR* HQMNFQKKTG*RCCVDM*LTPFVKQMLRILFILL IQNPLK*IQKIWTVH*VEHQEQ*KRLQKRLQEH SLSPKLQKELFEGLL*HPTAQWREEVLPAMISM **VVFLAHHH*QVSLLPPLSAFLPSLKGEVIR*V DLQLI*YEALPKS*IIEMYRHLILK*ETDLNGT CN*HLVKAGRKINNTKLCYLIFLLERVRFTCYI FKLIHVKNDSDFDVIYLLFESVIQRPII*VAIS* Y**LCNPDRVNKFYGRVPNTAVNLFV*YP*MNL WK*IFVQLNLCRD*MTS*YWMKTCIEF*LNSGS VSHVQFEVFK*PLLSQFIFFSSVFKI*HVDFKRF ALINKNNI*RRLFQNIFAN*DKDRKIEKHCIFC KNKMFVAVSERVRYIYGNFIH*QILI*FDSVWN FILKDKTMGKLW*RLFVPFMK*F*SCHQFY*SSV KCIDMRMFNFLLWSYN*M*N*KFICCFKV*YLS Q*PFYSQ*FRIIKFIWINAFLFPISLGSATNVC HLNFKFLF**LTDYRLFSMPCMCHF*E**MTLCY ILKAIVLVRTL*INT*NPSV

Sequence ECT2.hAug05

ECT2.F12 (forward primer)
ECT2.R11 (reverse primer)
Single exon skipping

Frame 0

RLTSRANRHGS*REQLSF*IGCGGRRRGMAVFV RGVGV*RGGTPRAFLRVTESTSCYLDWSAVARSR LTATSASRVQAILLPQPPE*LGLQVPATKPS*F LYF**RRGFIVLARLVSNS*PRDPPALASQSAR ITSVSHRVRPFKC*FRRIQIMAENSVLTSTTGRT SLADSSIFDSKVTEISKENLLIGSTSYVEEEMP QIETRVILVQEAGKQEELIKALKTIKIMEVPVI KIKESCPGKSDEKLIKSVINMDIKVGFVKMESVE EFEGLDSPEFENVFVVTDFQDSVFNDLYKADCR VIGPPVVLNC

Frame 1

G*HHAPIGMALRESSLVFESVVAAAGEEWRYL* EESAFEEVELLGLF*E*RSLPLVT*TGVQWHDL GSLQPLPPGFKRFSCLSLLSSWDYRCLPPSPANF CIFSRDGVSLCWPGWSRTPDLVIRPPWPPKVLG LQV*ATASGLSNADLEEYKSWLKIVY*HPLLGG LAWQTLPFLILKLLRFPRKTYLLDLLHM*KKRCL RLKQE*YWFKKLENKKNL*KP*RLLK*WKSLL* R*KKVVLENRMKN**KVLLIWTLKWAL*RWSQW KNLKVWILRNLKMYL*SRTFRILSLMTSTRLIVE LLDHQLY*I

Frame 2

VDITRQSAWLLERAA*FLNRLWRPPARNGGICE RSRRLKRWNS*GFFESDGVYLLLPRLECSGTIS AHCNLCLPGSSDSPASAS*VAGITGACHQAQLI FVFLVETGFHCVGQAGLELLTS*SARLGLPKC*D YKCEPPRPAFQMLI*KNTNHG*K*CINIHYWED *LGRLFHF*F*SY*DFQGKLTYWIYFICRRRDA SD*NKSDIGSRSWKTRRTYKSLKDY*NNGSPCYK DKRKLSWKIG*KINKKCY*YGH*SGLCKDGVSG RI*RFGFSGI*KCICSHGLSGFCL**PLQG*L* SYWTTSCIKL

Sequence ECT2.fAug05

AGGTTGACATCACGCGCCAATCGGCATGGCTCTTAGAGAGAGCAGCTTAGTTTTTGAATCGGTTGTGGCGGCCGCCGGCGAGGAATGGCGGTATTTGTGA GAGGAGTCGGCGTTTGAAGAGGTGGAACTCCTAGGGCTTTTTTGAGAGTGCTGATTTAGAAGAATACAAATCATGGCTGAAAATAGTGTATTAACATCCA CTACTGGGAGGACTAGCTTGGCAGACTCTTCCATTTTTGATTCTAAAGTTACTGAGATTTCCAAGGAAAACTTACTTATTGGATCTACTTCATATGTAGA AGAAGAGATGCCTCAGATTGAAACAAGAGTGATATTGGTTCAAGAAGCTGGAAAACAAGAAGAACTTATAAAAGCCTTAAAGACTATTAAAATAATGGAA GTCCCTGTTATAAAGATAAAAGAAAGTTGTCCTGGAAAATCGGATGAAAAATTAATAAAAAGTGTTATTAATATGGACATTAAAGTGGGCTTTGTAAAGA TGGAGTCAGTGGAAGAATTTGAAGGTTTGGATTCTCCGGAATTTGAAAATGTATTTGTAGTCACGGACTTTCAGGATTCTGTCTTTAATGACCTCTACAA GGCTGATTGTAGAGTTATTGGACCACCAGTTGTATTAAATTGT

ECT2.F12 (forward primer)
ECT2.R11 (reverse primer)
Single exon skipping

Frame 0

RLTSRANRHGS*REQLSF*IGCGGRRRGMAVFV RGVGV*RGGTPRAFLRVLI*KNTNHG*K*CINIH YWED*LGRLFHF*F*SY*DFQGKLTYWIYFICR RRDASD*NKSDIGSRSWKTRRTYKSLKDY*NNG SPCYKDKRKLSWKIG*KINKKCY*YGH*SGLCKD GVSGRI*RFGFSGI*KCICSHGLSGFCL**PLQ G*L*SYWTTSCIKL

Frame 1

G*HHAPIGMALRESSLVFESVVAAAGEEWRYL* EESAFEEVELLGLF*EC*FRRIQIMAENSVLTS TTGRTSLADSSIFDSKVTEISKENLLIGSTSYVE EEMPQIETRVILVQEAGKQEELIKALKTIKIME VPVIKIKESCPGKSDEKLIKSVINMDIKVGFVK MESVEEFEGLDSPEFENVFVVTDFQDSVFNDLYK ADCRVIGPPVVLNC

Frame 2

VDITRQSAWLLERAA*FLNRLWRPPARNGGICE RSRRLKRWNS*GFFESADLEEYKSWLKIVY*HP LLGGLAWQTLPFLILKLLRFPRKTYLLDLLHM* KKRCLRLKQE*YWFKKLENKKNL*KP*RLLK*WK SLL*R*KKVVLENRMKN**KVLLIWTLKWAL*R WSQWKNLKVWILRNLKMYL*SRTFRILSLMTST RLIVELLDHQLY*I