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Sequences

Sequence CD40.aAug05

GGCGGGGCCAAGGCTGGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGGCGCCCAGTGGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCGTCTGCCTCT GCAGTGCGTCCTCTGGGGCTGCTTGCTGACCGCTGTCCATCCAGAACCACCCACTGCATGCAGAGAAAAACAGTACCTAATAAACAGTCAGTGCTGTTCT TTGTGCCAGCCAGGACAGAAACTGGTGAGTGACTGCACAGAGTTCACTGAAACGGAATGCCTTCCTTGCGGTGAAAGCGAATTCCTAGACACCTGGAACA GAGAGACACACTGCCACCAGCACAAATACTGCGACCCCAACCTAGGGCTTCGGGTCCAGCAGAAGGGCACCTCAGAAACAGACACCATCTGCACCTGTGA AGAAGGCTGGCACTGTACGAGTGAGGCCTGTGAGAGCTGTGTCCTGCACCGCTCATGCTCGCCCGGCTTTGGGGTCAAGCAGATTGCTACAGGGGTTTCT GATACCATCTGCGAGCCCTGCCCAGTCGGCTTCTTCTCCAATGTGTCATCTGCTTTCGAAAAATGTCACCCTTGGACAAGCTGTGAGACCAAAGACCTGG TTGTGCAACAGGCAGGCACAAACAAGACTGATGTTGTCTGTGGTCCCCAGGATCGGCTGAGAGCCCTGGTGGTGATCCCCATCATCTTCGGGATCCTGTT TGCCATCCTCTTGGTGCTGGTCTTTATCAAAAAGGTGGCCAAGAAGCCAACCAATAAGGCCCCCCACCCCAAGCAGGAACCCCAGGAGATCAATTTTCCC GACGATCTTCCTGGCTCCAACACTGCTGCTCCAGTGCAGGAGACTTTACATGGATGCCAACCGGTCACCCAGGAGGATGGCAAAGAGAGTCGCATCTCAG TGCAGGAGAGACAGTGAGGCTGCACCCACCCAGGAGTGTGGCCACGTGGGCAAACAGGCAGTTGGCCAGAGAGCCTGGTGCTGCTGCTGCTGTGGCGTGA GGGTGAGGGGCTGGCACTGACTGGGCATAGCTCCCCGCTTCTGCCTGCACCCCTGCAGTTTGAGACAGGAGACCTGGCACTGGATGCAGAAACAGTTCAC CTTGAAGAACCTCTCACTTCACCCTGGAGCCCATCCAGTCTCCCAACTTGTATTAAAGACAGAGGCAGAAGTTTGGTGGTGGTGGTGTTGGGGTATGGTT TAGTAATATCCACCAGACCTTCCGATCCAGCAGTTTGGTGCCCAGAGAGGCATCATGGTGGCTTCCCTGCGCCCAGGAAGCCATATACACAGATGCCCAT TGCAGCATTGTTTGTGATAGTGAACAACTGGAAGCTGCTTAACTGTCCATCAGCAGGAGACTGGCTAAATAAAATTAGAATATATTTATACAACAGAATC TCAAAAACACTGTTGAGTAAGGAAAAAAAGGCATGCTGCTGAATGATGGGTATGGAACTTTTTAAAAAAGTACATGCTTTTATGTATGTATATTGCCTAT GGATATATGTATAAATACAATATGCATCATATATTGATATAACAAGGGTTCTGGAAGGGTACACAGAAAACCCACAGCTCGAAGAGTGGTGACGTCTGGG GTGGGGAAGAAGGGTCTGGGGGAGGGTTGGTTAAAGGGAGATTTGGCTTTCCCATAATGCTTCATCATTTTTCCCAAAAGGAGAGTGAATTCACATAATG CTTATGTAATTAAAAAATCATCAAACATGTA

CD40.F10 (forward primer)
CD40.R9 (reverse primer)
Alternative acceptor

Frame 0

GGAKAGAGESAEASLGRPVVLPPGLTSLWFVCL CSASSGAAC*PLSIQNHPLHAEKNST**TVSAVL CASQDRNW*VTAQSSLKRNAFLAVKANS*TPGT ERHTATSTNTATPT*GFGSSRRAPQKQTPSAPV KKAGTVRVRPVRAVSCTAHARPALGSSRLLQGFL IPSASPAQSASSPMCHLLSKNVTLGQAVRPKTW LCNRQAQTRLMLSVVPRIG*EPWW*SPSSSGSC LPSSWCWSLSKRWPRSQPIRPPTPSRNPRRSIFP TIFLAPTLLLQCRRLYMDANRSPRRMAKRVASQ CRRDSEAAPTQECGHVGKQAVGQRAWCCCCCGV RVRGWH*LGIAPRFCLHPCSLRQETWHWMQKQFT LKNLSLHPGAHPVSQLVLKTEAEVWWWWCWGMV **YPPDLPIQQFGAQRGIMVASLRPGSHIHRCP LQHCL***TTGSCLTVHQQETG*IKLEYIYTTES QKHC*VRKKRHAAE*WVWNFLKKYMLLCMYIAY GYMYKYNMHHILI*QGFWKGTQKTHSSKSGDVW GGEEGSGGGLVKGRFGFPIMLHHFSQKESEFT*C LCN*KIIKHV

Frame 1

AGPRLGQGSQQRPRSGAQWSCRLVSPRYGSSAS AVRPLGLLADRCPSRTTHCMQRKTVPNKQSVLF FVPARTETGE*LHRVH*NGMPSLR*KRIPRHLEQ RDTLPPAQILRPQPRASGPAEGHLRNRHHLHL* RRLALYE*GL*ELCPAPLMLARLWGQADCYRGF *YHLRALPSRLLLQCVICFRKMSPLDKL*DQRPG CATGRHKQD*CCLWSPGSAESPGGDPHHLRDPV CHPLGAGLYQKGGQEANQ*GPPPQAGTPGDQFS RRSSWLQHCCSSAGDFTWMPTGHPGGWQRESHLS AGETVRLHPPRSVATWANRQLAREPGAAAAVA* G*GAGTDWA*LPASACTPAV*DRRPGTGCRNSS P*RTSHFTLEPIQSPNLY*RQRQKFGGGGVGVWF SNIHQTFRSSSLVPREASWWLPCAQEAIYTDAH CSIVCDSEQLEAA*LSISRRLAK*N*NIFIQQN LKNTVE*GKKGMLLNDGYGTF*KSTCFYVCILPM DICINTICIIY*YNKGSGRVHRKPTARRVVTSG VGKKGLGEGWLKGDLAFP*CFIIFPKRRVNSHN AYVIKKSSNM

Frame 2

RGQGWGRGVSRGLARAPSGPAAWSHLAMVRLPL QCVLWGCLLTAVHPEPPTACREKQYLINSQCCS LCQPGQKLVSDCTEFTETECLPCGESEFLDTWN RETHCHQHKYCDPNLGLRVQQKGTSETDTICTCE EGWHCTSEACESCVLHRSCSPGFGVKQIATGVS DTICEPCPVGFFSNVSSAFEKCHPWTSCETKDL VVQQAGTNKTDVVCGPQDRLRALVVIPIIFGILF AILLVLVFIKKVAKKPTNKAPHPKQEPQEINFP DDLPGSNTAAPVQETLHGCQPVTQEDGKESRIS VQERQ*GCTHPGVWPRGQTGSWPESLVLLLLWRE GEGLALTGHSSPLLPAPLQFETGDLALDAETVH LEEPLTSPWSPSSLPTCIKDRGRSLVVVVLGYG LVISTRPSDPAVWCPERHHGGFPAPRKPYTQMPI AALFVIVNNWKLLNCPSAGDWLNKIRIYLYNRI SKTLLSKEKKACC*MMGMELFKKVHAFMYVYCL WIYV*IQYASYIDITRVLEGYTENPQLEEW*RLG WGRRVWGRVG*REIWLSHNASSFFPKGE*IHIM LM*LKNHQTC

Sequence CD40.bAug05

GGCGGGGCCAAGGCTGGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGGCGCCCAGTGGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCGTCTGCCTCT GCAGTGCGTCCTCTGGGGCTGCTTGCTGACCGCTGTCCATCCAGAACCACCCACTGCATGCAGAGAAAAACAGTACCTAATAAACAGTCAGTGCTGTTCT TTGTGCCAGCCAGGACAGAAACTGGTGAGTGACTGCACAGAGTTCACTGAAACGGAATGCCTTCCTTGCGGTGAAAGCGAATTCCTAGACACCTGGAACA GAGAGACACACTGCCACCAGCACAAATACTGCGACCCCAACCTAGGGCTTCGGGTCCAGCAGAAGGGCACCTCAGAAACAGACACCATCTGCACCTGTGA AGAAGGCTGGCACTGTACGAGTGAGGCCTGTGAGAGCTGTGTCCTGCACCGCTCATGCTCGCCCGGCTTTGGGGTCAAGCAGATTGCTACAGGGGTTTCT GATACCATCTGCGAGCCCTGCCCAGTCGGCTTCTTCTCCAATGTGTCATCTGCTTTCGAAAAATGTCACCCTTGGACAAGCTGTGAGACCAAAGACCTGG TTGTGCAACAGGCAGGCACAAACAAGACTGATGTTGTCTGTGGTGAGTCCTGGACAATGGGCCCTGGAGAAAGCCTAGGAAGGTCCCCAGGATCGGCTGA GAGCCCTGGTGGTGATCCCCATCATCTTCGGGATCCTGTTTGCCATCCTCTTGGTGCTGGTCTTTATCAAAAAGGTGGCCAAGAAGCCAACCAATAAGGC CCCCCACCCCAAGCAGGAACCCCAGGAGATCAATTTTCCCGACGATCTTCCTGGCTCCAACACTGCTGCTCCAGTGCAGGAGACTTTACATGGATGCCAA CCGGTCACCCAGGAGGATGGCAAAGAGAGTCGCATCTCAGTGCAGGAGAGACAGTGAGGCTGCACCCACCCAGGAGTGTGGCCACGTGGGCAAACAGGCA GTTGGCCAGAGAGCCTGGTGCTGCTGCTGCTGTGGCGTGAGGGTGAGGGGCTGGCACTGACTGGGCATAGCTCCCCGCTTCTGCCTGCACCCCTGCAGTT TGAGACAGGAGACCTGGCACTGGATGCAGAAACAGTTCACCTTGAAGAACCTCTCACTTCACCCTGGAGCCCATCCAGTCTCCCAACTTGTATTAAAGAC AGAGGCAGAAGTTTGGTGGTGGTGGTGTTGGGGTATGGTTTAGTAATATCCACCAGACCTTCCGATCCAGCAGTTTGGTGCCCAGAGAGGCATCATGGTG GCTTCCCTGCGCCCAGGAAGCCATATACACAGATGCCCATTGCAGCATTGTTTGTGATAGTGAACAACTGGAAGCTGCTTAACTGTCCATCAGCAGGAGA CTGGCTAAATAAAATTAGAATATATTTATACAACAGAATCTCAAAAACACTGTTGAGTAAGGAAAAAAAGGCATGCTGCTGAATGATGGGTATGGAACTT TTTAAAAAAGTACATGCTTTTATGTATGTATATTGCCTATGGATATATGTATAAATACAATATGCATCATATATTGATATAACAAGGGTTCTGGAAGGGT ACACAGAAAACCCACAGCTCGAAGAGTGGTGACGTCTGGGGTGGGGAAGAAGGGTCTGGGGGAGGGTTGGTTAAAGGGAGATTTGGCTTTCCCATAATGC TTCATCATTTTTCCCAAAAGGAGAGTGAATTCACATAATGCTTATGTAATTAAAAAATCATCAAACATGTA

CD40.F10 (forward primer)
CD40.R9 (reverse primer)
Alternative acceptor

Frame 0

GGAKAGAGESAEASLGRPVVLPPGLTSLWFVCL CSASSGAAC*PLSIQNHPLHAEKNST**TVSAVL CASQDRNW*VTAQSSLKRNAFLAVKANS*TPGT ERHTATSTNTATPT*GFGSSRRAPQKQTPSAPV KKAGTVRVRPVRAVSCTAHARPALGSSRLLQGFL IPSASPAQSASSPMCHLLSKNVTLGQAVRPKTW LCNRQAQTRLMLSVVSPGQWALEKA*EGPQDRL RALVVIPIIFGILFAILLVLVFIKKVAKKPTNKA PHPKQEPQEINFPDDLPGSNTAAPVQETLHGCQ PVTQEDGKESRISVQERQ*GCTHPGVWPRGQTG SWPESLVLLLLWREGEGLALTGHSSPLLPAPLQF ETGDLALDAETVHLEEPLTSPWSPSSLPTCIKD RGRSLVVVVLGYGLVISTRPSDPAVWCPERHHG GFPAPRKPYTQMPIAALFVIVNNWKLLNCPSAGD WLNKIRIYLYNRISKTLLSKEKKACC*MMGMEL FKKVHAFMYVYCLWIYV*IQYASYIDITRVLEG YTENPQLEEW*RLGWGRRVWGRVG*REIWLSHNA SSFFPKGE*IHIMLM*LKNHQTC

Frame 1

AGPRLGQGSQQRPRSGAQWSCRLVSPRYGSSAS AVRPLGLLADRCPSRTTHCMQRKTVPNKQSVLF FVPARTETGE*LHRVH*NGMPSLR*KRIPRHLEQ RDTLPPAQILRPQPRASGPAEGHLRNRHHLHL* RRLALYE*GL*ELCPAPLMLARLWGQADCYRGF *YHLRALPSRLLLQCVICFRKMSPLDKL*DQRPG CATGRHKQD*CCLW*VLDNGPWRKPRKVPRIG* EPWW*SPSSSGSCLPSSWCWSLSKRWPRSQPIR PPTPSRNPRRSIFPTIFLAPTLLLQCRRLYMDAN RSPRRMAKRVASQCRRDSEAAPTQECGHVGKQA VGQRAWCCCCCGVRVRGWH*LGIAPRFCLHPCS LRQETWHWMQKQFTLKNLSLHPGAHPVSQLVLKT EAEVWWWWCWGMV**YPPDLPIQQFGAQRGIMV ASLRPGSHIHRCPLQHCL***TTGSCLTVHQQE TG*IKLEYIYTTESQKHC*VRKKRHAAE*WVWNF LKKYMLLCMYIAYGYMYKYNMHHILI*QGFWKG TQKTHSSKSGDVWGGEEGSGGGLVKGRFGFPIM LHHFSQKESEFT*CLCN*KIIKHV

Frame 2

RGQGWGRGVSRGLARAPSGPAAWSHLAMVRLPL QCVLWGCLLTAVHPEPPTACREKQYLINSQCCS LCQPGQKLVSDCTEFTETECLPCGESEFLDTWN RETHCHQHKYCDPNLGLRVQQKGTSETDTICTCE EGWHCTSEACESCVLHRSCSPGFGVKQIATGVS DTICEPCPVGFFSNVSSAFEKCHPWTSCETKDL VVQQAGTNKTDVVCGESWTMGPGESLGRSPGSAE SPGGDPHHLRDPVCHPLGAGLYQKGGQEANQ*G PPPQAGTPGDQFSRRSSWLQHCCSSAGDFTWMP TGHPGGWQRESHLSAGETVRLHPPRSVATWANRQ LAREPGAAAAVA*G*GAGTDWA*LPASACTPAV *DRRPGTGCRNSSP*RTSHFTLEPIQSPNLY*R QRQKFGGGGVGVWFSNIHQTFRSSSLVPREASWW LPCAQEAIYTDAHCSIVCDSEQLEAA*LSISRR LAK*N*NIFIQQNLKNTVE*GKKGMLLNDGYGT F*KSTCFYVCILPMDICINTICIIY*YNKGSGRV HRKPTARRVVTSGVGKKGLGEGWLKGDLAFP*C FIIFPKRRVNSHNAYVIKKSSNM

Sequence CD40.cAug05

GGCGGGGCCAAGGCTGGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGGCGCCCAGTGGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCGTCTGCCTCT GCAGTGCGTCCTCTGGGGCTGCTTGCTGACCGCTGTCCATCCAGAACCACCCACTGCATGCAGAGAAAAACAGTACCTAATAAACAGTCAGTGCTGTTCT TTGTGCCAGCCAGGACAGAAACTGGTGAGTGACTGCACAGAGTTCACTGAAACGGAATGCCTTCCTTGCGGTGAAAGCGAATTCCTAGACACCTGGAACA GAGAGACACACTGCCACCAGCACAAATACTGCGACCCCAACCTAGGGCTTCGGGTCCAGCAGAAGGGCACCTCAGAAACAGACACCATCTGCACCTGTGA AGAAGGCTGGCACTGTACGAGTGAGGCCTGTGAGAGCTGTGTCCTGCACCGCTCATGCTCGCCCGGCTTTGGGGTCAAGCAGATTGCTACAGGGGTTTCT GATACCATCTGCGAGCCCTGCCCAGTCGGCTTCTTCTCCAATGTGTCATCTGCTTTCGAAAAATGTCACCCTTGGACAAGGTCCCCAGGATCGGCTGAGA GCCCTGGTGGTGATCCCCATCATCTTCGGGATCCTGTTTGCCATCCTCTTGGTGCTGGTCTTTATCAAAAAGGTGGCCAAGAAGCCAACCAATAAGGCCC CCCACCCCAAGCAGGAACCCCAGGAGATCAATTTTCCCGACGATCTTCCTGGCTCCAACACTGCTGCTCCAGTGCAGGAGACTTTACATGGATGCCAACC GGTCACCCAGGAGGATGGCAAAGAGAGTCGCATCTCAGTGCAGGAGAGACAGTGAGGCTGCACCCACCCAGGAGTGTGGCCACGTGGGCAAACAGGCAGT TGGCCAGAGAGCCTGGTGCTGCTGCTGCTGTGGCGTGAGGGTGAGGGGCTGGCACTGACTGGGCATAGCTCCCCGCTTCTGCCTGCACCCCTGCAGTTTG AGACAGGAGACCTGGCACTGGATGCAGAAACAGTTCACCTTGAAGAACCTCTCACTTCACCCTGGAGCCCATCCAGTCTCCCAACTTGTATTAAAGACAG AGGCAGAAGTTTGGTGGTGGTGGTGTTGGGGTATGGTTTAGTAATATCCACCAGACCTTCCGATCCAGCAGTTTGGTGCCCAGAGAGGCATCATGGTGGC TTCCCTGCGCCCAGGAAGCCATATACACAGATGCCCATTGCAGCATTGTTTGTGATAGTGAACAACTGGAAGCTGCTTAACTGTCCATCAGCAGGAGACT GGCTAAATAAAATTAGAATATATTTATACAACAGAATCTCAAAAACACTGTTGAGTAAGGAAAAAAAGGCATGCTGCTGAATGATGGGTATGGAACTTTT TAAAAAAGTACATGCTTTTATGTATGTATATTGCCTATGGATATATGTATAAATACAATATGCATCATATATTGATATAACAAGGGTTCTGGAAGGGTAC ACAGAAAACCCACAGCTCGAAGAGTGGTGACGTCTGGGGTGGGGAAGAAGGGTCTGGGGGAGGGTTGGTTAAAGGGAGATTTGGCTTTCCCATAATGCTT CATCATTTTTCCCAAAAGGAGAGTGAATTCACATAATGCTTATGTAATTAAAAAATCATCAAACATGTA

CD40.F10 (forward primer)
CD40.R9 (reverse primer)
Alternative acceptor

Frame 0

GGAKAGAGESAEASLGRPVVLPPGLTSLWFVCL CSASSGAAC*PLSIQNHPLHAEKNST**TVSAVL CASQDRNW*VTAQSSLKRNAFLAVKANS*TPGT ERHTATSTNTATPT*GFGSSRRAPQKQTPSAPV KKAGTVRVRPVRAVSCTAHARPALGSSRLLQGFL IPSASPAQSASSPMCHLLSKNVTLGQGPQDRLR ALVVIPIIFGILFAILLVLVFIKKVAKKPTNKA PHPKQEPQEINFPDDLPGSNTAAPVQETLHGCQP VTQEDGKESRISVQERQ*GCTHPGVWPRGQTGS WPESLVLLLLWREGEGLALTGHSSPLLPAPLQF ETGDLALDAETVHLEEPLTSPWSPSSLPTCIKDR GRSLVVVVLGYGLVISTRPSDPAVWCPERHHGG FPAPRKPYTQMPIAALFVIVNNWKLLNCPSAGD WLNKIRIYLYNRISKTLLSKEKKACC*MMGMELF KKVHAFMYVYCLWIYV*IQYASYIDITRVLEGY TENPQLEEW*RLGWGRRVWGRVG*REIWLSHNA SSFFPKGE*IHIMLM*LKNHQTC

Frame 1

AGPRLGQGSQQRPRSGAQWSCRLVSPRYGSSAS AVRPLGLLADRCPSRTTHCMQRKTVPNKQSVLF FVPARTETGE*LHRVH*NGMPSLR*KRIPRHLEQ RDTLPPAQILRPQPRASGPAEGHLRNRHHLHL* RRLALYE*GL*ELCPAPLMLARLWGQADCYRGF *YHLRALPSRLLLQCVICFRKMSPLDKVPRIG*E PWW*SPSSSGSCLPSSWCWSLSKRWPRSQPIRP PTPSRNPRRSIFPTIFLAPTLLLQCRRLYMDAN RSPRRMAKRVASQCRRDSEAAPTQECGHVGKQAV GQRAWCCCCCGVRVRGWH*LGIAPRFCLHPCSL RQETWHWMQKQFTLKNLSLHPGAHPVSQLVLKT EAEVWWWWCWGMV**YPPDLPIQQFGAQRGIMVA SLRPGSHIHRCPLQHCL***TTGSCLTVHQQET G*IKLEYIYTTESQKHC*VRKKRHAAE*WVWNF LKKYMLLCMYIAYGYMYKYNMHHILI*QGFWKGT QKTHSSKSGDVWGGEEGSGGGLVKGRFGFPIML HHFSQKESEFT*CLCN*KIIKHV

Frame 2

RGQGWGRGVSRGLARAPSGPAAWSHLAMVRLPL QCVLWGCLLTAVHPEPPTACREKQYLINSQCCS LCQPGQKLVSDCTEFTETECLPCGESEFLDTWN RETHCHQHKYCDPNLGLRVQQKGTSETDTICTCE EGWHCTSEACESCVLHRSCSPGFGVKQIATGVS DTICEPCPVGFFSNVSSAFEKCHPWTRSPGSAE SPGGDPHHLRDPVCHPLGAGLYQKGGQEANQ*GP PPQAGTPGDQFSRRSSWLQHCCSSAGDFTWMPT GHPGGWQRESHLSAGETVRLHPPRSVATWANRQ LAREPGAAAAVA*G*GAGTDWA*LPASACTPAV* DRRPGTGCRNSSP*RTSHFTLEPIQSPNLY*RQ RQKFGGGGVGVWFSNIHQTFRSSSLVPREASWW LPCAQEAIYTDAHCSIVCDSEQLEAA*LSISRRL AK*N*NIFIQQNLKNTVE*GKKGMLLNDGYGTF *KSTCFYVCILPMDICINTICIIY*YNKGSGRV HRKPTARRVVTSGVGKKGLGEGWLKGDLAFP*CF IIFPKRRVNSHNAYVIKKSSNM

Sequence CD40.eAug05

GGCGGGGCCAAGGCTGGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGGCGCCCAGTGGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCGTCTGCCTCT GCAGTGCGTCCTCTGGGGCTGCTTGCTGACCGCTGTCCATCCAGAACCACCCACTGCATGCAGAGAAAAACAGTACCTAATAAACAGTCAGTGCTGTTCT TTGTGCCAGCCAGGACAGAAACTGGTGAGTGACTGCACAGAGTTCACTGAAACGGAATGCCTTCCTTGCGGTGAAAGCGAATTCCTAGACACCTGGAACA GAGAGACACACTGCCACCAGCACAAATACTGCGACCCCAACCTAGGGCTTCGGGTCCAGCAGAAGGGCACCTCAGAAACAGACACCATCTGCACCTGTGA AGAAGGCTGGCACTGTACGAGTGAGGCCTGTGAGAGCTGTGTCCTGCACCGCTCATGCTCGCCCGGCTTTGGGGTCAAGCAGATTGCTGTGAGACCAAAG ACCTGGTTGTGCAACAGGCAGGCACAAACAAGACTGATGTTGTCTGTGGTCCCCAGGATCGGCTGAGAGCCCTGGTGGTGATCCCCATCATCTTCGGGAT CCTGTTTGCCATCCTCTTGGTGCTGGTCTTTATCAAAAAGGTGGCCAAGAAGCCAACCAATAAGGCCCCCCACCCCAAGCAGGAACCCCAGGAGATCAAT TTTCCCGACGATCTTCCTGGCTCCAACACTGCTGCTCCAGTGCAGGAGACTTTACATGGATGCCAACCGGTCACCCAGGAGGATGGCAAAGAGAGTCGCA TCTCAGTGCAGGAGAGACAGTGAGGCTGCACCCACCCAGGAGTGTGGCCACGTGGGCAAACAGGCAGTTGGCCAGAGAGCCTGGTGCTGCTGCTGCTGTG GCGTGAGGGTGAGGGGCTGGCACTGACTGGGCATAGCTCCCCGCTTCTGCCTGCACCCCTGCAGTTTGAGACAGGAGACCTGGCACTGGATGCAGAAACA GTTCACCTTGAAGAACCTCTCACTTCACCCTGGAGCCCATCCAGTCTCCCAACTTGTATTAAAGACAGAGGCAGAAGTTTGGTGGTGGTGGTGTTGGGGT ATGGTTTAGTAATATCCACCAGACCTTCCGATCCAGCAGTTTGGTGCCCAGAGAGGCATCATGGTGGCTTCCCTGCGCCCAGGAAGCCATATACACAGAT GCCCATTGCAGCATTGTTTGTGATAGTGAACAACTGGAAGCTGCTTAACTGTCCATCAGCAGGAGACTGGCTAAATAAAATTAGAATATATTTATACAAC AGAATCTCAAAAACACTGTTGAGTAAGGAAAAAAAGGCATGCTGCTGAATGATGGGTATGGAACTTTTTAAAAAAGTACATGCTTTTATGTATGTATATT GCCTATGGATATATGTATAAATACAATATGCATCATATATTGATATAACAAGGGTTCTGGAAGGGTACACAGAAAACCCACAGCTCGAAGAGTGGTGACG TCTGGGGTGGGGAAGAAGGGTCTGGGGGAGGGTTGGTTAAAGGGAGATTTGGCTTTCCCATAATGCTTCATCATTTTTCCCAAAAGGAGAGTGAATTCAC ATAATGCTTATGTAATTAAAAAATCATCAAACATGTA

CD40.F10 (forward primer)
CD40.R9 (reverse primer)
Alternative acceptor

Frame 0

GGAKAGAGESAEASLGRPVVLPPGLTSLWFVCL CSASSGAAC*PLSIQNHPLHAEKNST**TVSAVL CASQDRNW*VTAQSSLKRNAFLAVKANS*TPGT ERHTATSTNTATPT*GFGSSRRAPQKQTPSAPV KKAGTVRVRPVRAVSCTAHARPALGSSRLL*DQR PGCATGRHKQD*CCLWSPGSAESPGGDPHHLRD PVCHPLGAGLYQKGGQEANQ*GPPPQAGTPGDQ FSRRSSWLQHCCSSAGDFTWMPTGHPGGWQRESH LSAGETVRLHPPRSVATWANRQLAREPGAAAAV A*G*GAGTDWA*LPASACTPAV*DRRPGTGCRN SSP*RTSHFTLEPIQSPNLY*RQRQKFGGGGVGV WFSNIHQTFRSSSLVPREASWWLPCAQEAIYTD AHCSIVCDSEQLEAA*LSISRRLAK*N*NIFIQ QNLKNTVE*GKKGMLLNDGYGTF*KSTCFYVCIL PMDICINTICIIY*YNKGSGRVHRKPTARRVVT SGVGKKGLGEGWLKGDLAFP*CFIIFPKRRVNS HNAYVIKKSSNM

Frame 1

AGPRLGQGSQQRPRSGAQWSCRLVSPRYGSSAS AVRPLGLLADRCPSRTTHCMQRKTVPNKQSVLF FVPARTETGE*LHRVH*NGMPSLR*KRIPRHLEQ RDTLPPAQILRPQPRASGPAEGHLRNRHHLHL* RRLALYE*GL*ELCPAPLMLARLWGQADCCETK DLVVQQAGTNKTDVVCGPQDRLRALVVIPIIFGI LFAILLVLVFIKKVAKKPTNKAPHPKQEPQEIN FPDDLPGSNTAAPVQETLHGCQPVTQEDGKESR ISVQERQ*GCTHPGVWPRGQTGSWPESLVLLLLW REGEGLALTGHSSPLLPAPLQFETGDLALDAET VHLEEPLTSPWSPSSLPTCIKDRGRSLVVVVLG YGLVISTRPSDPAVWCPERHHGGFPAPRKPYTQM PIAALFVIVNNWKLLNCPSAGDWLNKIRIYLYN RISKTLLSKEKKACC*MMGMELFKKVHAFMYVY CLWIYV*IQYASYIDITRVLEGYTENPQLEEW*R LGWGRRVWGRVG*REIWLSHNASSFFPKGE*IH IMLM*LKNHQTC

Frame 2

RGQGWGRGVSRGLARAPSGPAAWSHLAMVRLPL QCVLWGCLLTAVHPEPPTACREKQYLINSQCCS LCQPGQKLVSDCTEFTETECLPCGESEFLDTWN RETHCHQHKYCDPNLGLRVQQKGTSETDTICTCE EGWHCTSEACESCVLHRSCSPGFGVKQIAVRPK TWLCNRQAQTRLMLSVVPRIG*EPWW*SPSSSG SCLPSSWCWSLSKRWPRSQPIRPPTPSRNPRRSI FPTIFLAPTLLLQCRRLYMDANRSPRRMAKRVA SQCRRDSEAAPTQECGHVGKQAVGQRAWCCCCC GVRVRGWH*LGIAPRFCLHPCSLRQETWHWMQKQ FTLKNLSLHPGAHPVSQLVLKTEAEVWWWWCWG MV**YPPDLPIQQFGAQRGIMVASLRPGSHIHR CPLQHCL***TTGSCLTVHQQETG*IKLEYIYTT ESQKHC*VRKKRHAAE*WVWNFLKKYMLLCMYI AYGYMYKYNMHHILI*QGFWKGTQKTHSSKSGD VWGGEEGSGGGLVKGRFGFPIMLHHFSQKESEFT *CLCN*KIIKHV

Sequence CD40.fAug05

GGCGGGGCCAAGGCTGGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGGCGCCCAGTGGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCGTCTGCCTCT GCAGTGCGTCCTCTGGGGCTGCTTGCTGACCGCTGTCCATCCAGAACCACCCACTGCATGCAGAGAAAAACAGTACCTAATAAACAGTCAGTGCTGTTCT TTGTGCCAGCCAGGACAGAAACTGGTGAGTGACTGCACAGAGTTCACTGAAACGGAATGCCTTCCTTGCGGTGAAAGCGAATTCCTAGACACCTGGAACA GAGAGACACACTGCCACCAGCACAAATACTGCGACCCCAACCTAGGGCTTCGGGTCCAGCAGAAGGGCACCTCAGAAACAGACACCATCTGCACCTGTGA AGAAGGCTGGCACTGTACGAGTGAGGCCTGTGAGAGCTGTGTCCTGCACCGCTCATGCTCGCCCGGCTTTGGGGTCAAGCAGATTGACATCTGCCAGCCA CATTTCCCCAAGGACCGCGGTTTGAACCTTCTGATGTAGATGAGCTCTGACATTGGAAGATTCTGGAGTCTGACAAGTCACAGCAGGTTGAGGGTAGGGA GAAACTGCAGGTGAGGGGTGCATGCTGAAGTCCTGATTTCTCCAGGTCCCCAGGATCGGCTGAGAGCCCTGGTGGTGATCCCCATCATCTTCGGGATCCT GTTTGCCATCCTCTTGGTGCTGGTCTTTATCAAAAAGGTGGCCAAGAAGCCAACCAATAAGGCCCCCCACCCCAAGCAGGAACCCCAGGAGATCAATTTT CCCGACGATCTTCCTGGCTCCAACACTGCTGCTCCAGTGCAGGAGACTTTACATGGATGCCAACCGGTCACCCAGGAGGATGGCAAAGAGAGTCGCATCT CAGTGCAGGAGAGACAGTGAGGCTGCACCCACCCAGGAGTGTGGCCACGTGGGCAAACAGGCAGTTGGCCAGAGAGCCTGGTGCTGCTGCTGCTGTGGCG TGAGGGTGAGGGGCTGGCACTGACTGGGCATAGCTCCCCGCTTCTGCCTGCACCCCTGCAGTTTGAGACAGGAGACCTGGCACTGGATGCAGAAACAGTT CACCTTGAAGAACCTCTCACTTCACCCTGGAGCCCATCCAGTCTCCCAACTTGTATTAAAGACAGAGGCAGAAGTTTGGTGGTGGTGGTGTTGGGGTATG GTTTAGTAATATCCACCAGACCTTCCGATCCAGCAGTTTGGTGCCCAGAGAGGCATCATGGTGGCTTCCCTGCGCCCAGGAAGCCATATACACAGATGCC CATTGCAGCATTGTTTGTGATAGTGAACAACTGGAAGCTGCTTAACTGTCCATCAGCAGGAGACTGGCTAAATAAAATTAGAATATATTTATACAACAGA ATCTCAAAAACACTGTTGAGTAAGGAAAAAAAGGCATGCTGCTGAATGATGGGTATGGAACTTTTTAAAAAAGTACATGCTTTTATGTATGTATATTGCC TATGGATATATGTATAAATACAATATGCATCATATATTGATATAACAAGGGTTCTGGAAGGGTACACAGAAAACCCACAGCTCGAAGAGTGGTGACGTCT GGGGTGGGGAAGAAGGGTCTGGGGGAGGGTTGGTTAAAGGGAGATTTGGCTTTCCCATAATGCTTCATCATTTTTCCCAAAAGGAGAGTGAATTCACATA ATGCTTATGTAATTAAAAAATCATCAAACATGTA

CD40.F10 (forward primer)
CD40.R9 (reverse primer)
Alternative acceptor

Frame 0

GGAKAGAGESAEASLGRPVVLPPGLTSLWFVCL CSASSGAAC*PLSIQNHPLHAEKNST**TVSAVL CASQDRNW*VTAQSSLKRNAFLAVKANS*TPGT ERHTATSTNTATPT*GFGSSRRAPQKQTPSAPV KKAGTVRVRPVRAVSCTAHARPALGSSRLTSASH ISPRTAV*TF*CR*ALTLEDSGV*QVTAG*G*G ETAGEGCMLKS*FLQVPRIG*EPWW*SPSSSGS CLPSSWCWSLSKRWPRSQPIRPPTPSRNPRRSIF PTIFLAPTLLLQCRRLYMDANRSPRRMAKRVAS QCRRDSEAAPTQECGHVGKQAVGQRAWCCCCCG VRVRGWH*LGIAPRFCLHPCSLRQETWHWMQKQF TLKNLSLHPGAHPVSQLVLKTEAEVWWWWCWGM V**YPPDLPIQQFGAQRGIMVASLRPGSHIHRC PLQHCL***TTGSCLTVHQQETG*IKLEYIYTTE SQKHC*VRKKRHAAE*WVWNFLKKYMLLCMYIA YGYMYKYNMHHILI*QGFWKGTQKTHSSKSGDV WGGEEGSGGGLVKGRFGFPIMLHHFSQKESEFT* CLCN*KIIKHV

Frame 1

AGPRLGQGSQQRPRSGAQWSCRLVSPRYGSSAS AVRPLGLLADRCPSRTTHCMQRKTVPNKQSVLF FVPARTETGE*LHRVH*NGMPSLR*KRIPRHLEQ RDTLPPAQILRPQPRASGPAEGHLRNRHHLHL* RRLALYE*GL*ELCPAPLMLARLWGQAD*HLPA TFPQGPRFEPSDVDEL*HWKILESDKSQQVEGRE KLQVRGAC*SPDFSRSPGSAESPGGDPHHLRDP VCHPLGAGLYQKGGQEANQ*GPPPQAGTPGDQF SRRSSWLQHCCSSAGDFTWMPTGHPGGWQRESHL SAGETVRLHPPRSVATWANRQLAREPGAAAAVA *G*GAGTDWA*LPASACTPAV*DRRPGTGCRNS SP*RTSHFTLEPIQSPNLY*RQRQKFGGGGVGVW FSNIHQTFRSSSLVPREASWWLPCAQEAIYTDA HCSIVCDSEQLEAA*LSISRRLAK*N*NIFIQQ NLKNTVE*GKKGMLLNDGYGTF*KSTCFYVCILP MDICINTICIIY*YNKGSGRVHRKPTARRVVTS GVGKKGLGEGWLKGDLAFP*CFIIFPKRRVNSH NAYVIKKSSNM

Frame 2

RGQGWGRGVSRGLARAPSGPAAWSHLAMVRLPL QCVLWGCLLTAVHPEPPTACREKQYLINSQCCS LCQPGQKLVSDCTEFTETECLPCGESEFLDTWN RETHCHQHKYCDPNLGLRVQQKGTSETDTICTCE EGWHCTSEACESCVLHRSCSPGFGVKQIDICQP HFPKDRGLNLLM*MSSDIGRFWSLTSHSRLRVG RNCR*GVHAEVLISPGPQDRLRALVVIPIIFGIL FAILLVLVFIKKVAKKPTNKAPHPKQEPQEINF PDDLPGSNTAAPVQETLHGCQPVTQEDGKESRI SVQERQ*GCTHPGVWPRGQTGSWPESLVLLLLWR EGEGLALTGHSSPLLPAPLQFETGDLALDAETV HLEEPLTSPWSPSSLPTCIKDRGRSLVVVVLGY GLVISTRPSDPAVWCPERHHGGFPAPRKPYTQMP IAALFVIVNNWKLLNCPSAGDWLNKIRIYLYNR ISKTLLSKEKKACC*MMGMELFKKVHAFMYVYC LWIYV*IQYASYIDITRVLEGYTENPQLEEW*RL GWGRRVWGRVG*REIWLSHNASSFFPKGE*IHI MLM*LKNHQTC

Sequence CD40.iAug05

GGCGGGGCCAAGGCTGGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGGCGCCCAGTGGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCGTCTGCCTCT GCAGTGCGTCCTCTGGGGCTGCTTGCTGACCGCTTTTGCAGCCTCTGTCCTGGATGACTTTTACCTTTCTGCTGAAGAAGTTGCAACGGAGATTTCAAGA TCCCTTCAAATTGCACAATTCTGTTTTTAGGTCCATCCAGAACCACCCACTGCATGCAGAGAAAAACAGTACCTAATAAACAGTCAGTGCTGTTCTTTGT GCCAGCCAGGACAGAAACTGGTGAGTGACTGCACAGAGTTCACTGAAACGGAATGCCTTCCTTGCGGTGAAAGCGAATTCCTAGACACCTGGAACAGAGA GACACACTGCCACCAGCACAAATACTGCGACCCCAACCTAGGGCTTCGGGTCCAGCAGAAGGGCACCTCAGAAACAGACACCATCTGCACCTGTGAAGAA GGCTGGCACTGTACGAGTGAGGCCTGTGAGAGCTGTGTCCTGCACCGCTCATGCTCGCCCGGCTTTGGGGTCAAGCAGATTGCTGTGAGACCAAAGACCT GGTTGTGCAACAGGCAGGCACAAACAAGACTGATGTTGTCTGTGGTCCCCAGGATCGGCTGAGAGCCCTGGTGGTGATCCCCATCATCTTCGGGATCCTG TTTGCCATCCTCTTGGTGCTGGTCTTTATCAAAAAGGTGGCCAAGAAGCCAACCAATAAGGCCCCCCACCCCAAGCAGGAACCCCAGGAGATCAATTTTC CCGACGATCTTCCTGGCTCCAACACTGCTGCTCCAGTGCAGGAGACTTTACATGGATGCCAACCGGTCACCCAGGAGGATGGCAAAGAGAGTCGCATCTC AGTGCAGGAGAGACAGTGAGGCTGCACCCACCCAGGAGTGTGGCCACGTGGGCAAACAGGCAGTTGGCCAGAGAGCCTGGTGCTGCTGCTGCTGTGGCGT GAGGGTGAGGGGCTGGCACTGACTGGGCATAGCTCCCCGCTTCTGCCTGCACCCCTGCAGTTTGAGACAGGAGACCTGGCACTGGATGCAGAAACAGTTC ACCTTGAAGAACCTCTCACTTCACCCTGGAGCCCATCCAGTCTCCCAACTTGTATTAAAGACAGAGGCAGAAGTTTGGTGGTGGTGGTGTTGGGGTATGG TTTAGTAATATCCACCAGACCTTCCGATCCAGCAGTTTGGTGCCCAGAGAGGCATCATGGTGGCTTCCCTGCGCCCAGGAAGCCATATACACAGATGCCC ATTGCAGCATTGTTTGTGATAGTGAACAACTGGAAGCTGCTTAACTGTCCATCAGCAGGAGACTGGCTAAATAAAATTAGAATATATTTATACAACAGAA TCTCAAAAACACTGTTGAGTAAGGAAAAAAAGGCATGCTGCTGAATGATGGGTATGGAACTTTTTAAAAAAGTACATGCTTTTATGTATGTATATTGCCT ATGGATATATGTATAAATACAATATGCATCATATATTGATATAACAAGGGTTCTGGAAGGGTACACAGAAAACCCACAGCTCGAAGAGTGGTGACGTCTG GGGTGGGGAAGAAGGGTCTGGGGGAGGGTTGGTTAAAGGGAGATTTGGCTTTCCCATAATGCTTCATCATTTTTCCCAAAAGGAGAGTGAATTCACATAA TGCTTATGTAATTAAAAAATCATCAAACATGTA

CD40.F10 (forward primer)
CD40.R9 (reverse primer)
Alternative acceptor

Frame 0

GGAKAGAGESAEASLGRPVVLPPGLTSLWFVCL CSASSGAAC*PLLQPLSWMTFTFLLKKLQRRFQD PFKLHNSVFRSIQNHPLHAEKNST**TVSAVLC ASQDRNW*VTAQSSLKRNAFLAVKANS*TPGTE RHTATSTNTATPT*GFGSSRRAPQKQTPSAPVKK AGTVRVRPVRAVSCTAHARPALGSSRLL*DQRP GCATGRHKQD*CCLWSPGSAESPGGDPHHLRDP VCHPLGAGLYQKGGQEANQ*GPPPQAGTPGDQFS RRSSWLQHCCSSAGDFTWMPTGHPGGWQRESHL SAGETVRLHPPRSVATWANRQLAREPGAAAAVA *G*GAGTDWA*LPASACTPAV*DRRPGTGCRNSS P*RTSHFTLEPIQSPNLY*RQRQKFGGGGVGVW FSNIHQTFRSSSLVPREASWWLPCAQEAIYTDA HCSIVCDSEQLEAA*LSISRRLAK*N*NIFIQQN LKNTVE*GKKGMLLNDGYGTF*KSTCFYVCILP MDICINTICIIY*YNKGSGRVHRKPTARRVVTS GVGKKGLGEGWLKGDLAFP*CFIIFPKRRVNSHN AYVIKKSSNM

Frame 1

AGPRLGQGSQQRPRSGAQWSCRLVSPRYGSSAS AVRPLGLLADRFCSLCPG*LLPFC*RSCNGDFK IPSNCTILFLGPSRTTHCMQRKTVPNKQSVLFFV PARTETGE*LHRVH*NGMPSLR*KRIPRHLEQR DTLPPAQILRPQPRASGPAEGHLRNRHHLHL*R RLALYE*GL*ELCPAPLMLARLWGQADCCETKDL VVQQAGTNKTDVVCGPQDRLRALVVIPIIFGIL FAILLVLVFIKKVAKKPTNKAPHPKQEPQEINF PDDLPGSNTAAPVQETLHGCQPVTQEDGKESRIS VQERQ*GCTHPGVWPRGQTGSWPESLVLLLLWR EGEGLALTGHSSPLLPAPLQFETGDLALDAETV HLEEPLTSPWSPSSLPTCIKDRGRSLVVVVLGYG LVISTRPSDPAVWCPERHHGGFPAPRKPYTQMP IAALFVIVNNWKLLNCPSAGDWLNKIRIYLYNR ISKTLLSKEKKACC*MMGMELFKKVHAFMYVYCL WIYV*IQYASYIDITRVLEGYTENPQLEEW*RL GWGRRVWGRVG*REIWLSHNASSFFPKGE*IHI MLM*LKNHQTC

Frame 2

RGQGWGRGVSRGLARAPSGPAAWSHLAMVRLPL QCVLWGCLLTAFAASVLDDFYLSAEEVATEISR SLQIAQFCF*VHPEPPTACREKQYLINSQCCSL CQPGQKLVSDCTEFTETECLPCGESEFLDTWNRE THCHQHKYCDPNLGLRVQQKGTSETDTICTCEE GWHCTSEACESCVLHRSCSPGFGVKQIAVRPKT WLCNRQAQTRLMLSVVPRIG*EPWW*SPSSSGSC LPSSWCWSLSKRWPRSQPIRPPTPSRNPRRSIF PTIFLAPTLLLQCRRLYMDANRSPRRMAKRVAS QCRRDSEAAPTQECGHVGKQAVGQRAWCCCCCGV RVRGWH*LGIAPRFCLHPCSLRQETWHWMQKQF TLKNLSLHPGAHPVSQLVLKTEAEVWWWWCWGM V**YPPDLPIQQFGAQRGIMVASLRPGSHIHRCP LQHCL***TTGSCLTVHQQETG*IKLEYIYTTE SQKHC*VRKKRHAAE*WVWNFLKKYMLLCMYIA YGYMYKYNMHHILI*QGFWKGTQKTHSSKSGDVW GGEEGSGGGLVKGRFGFPIMLHHFSQKESEFT* CLCN*KIIKHV