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Sequences

Sequence DRF1.eAug05

GGATGCCGGGAAGGGGAGAAAGAAAGGCGGAAGGGGTCGTTAATAGCTGAGGCGAGGTGCGGAGTCGTGGAGGGGGCTTAGGAAGTGACCAGCGGGAGAC GATTGCCTCGAGCTGGAGAGTTCCATGGCTGAGAGTAGGCTCCGGGCCCCGGACCTAGGAGTTTCCAGGTGTCTAGGAAAATGCCAGAAGAACTCACCAG GTGCCAGGAAGCATCCCTTTTCCGGAAAGTCCTTTTACTTGGATCTGCCTGCTGGCAAGAATCTCCAGTTTTTGACGGGGGCCATTCAGCAACTGGGTGG GGTAATTGAGGGTTTTCTGAGCAAAGAAGTAAGTTACATCGTGTCCAGCCGCAGAGAAGTAAAGGCAGAGAGCAGTGGGAAAAGCCATAGAGGCTGCCCT AGCCCTAGCCCCAGTGAGGTCAGAGTGGAAACATCGGCCATGGTTGATCCAAAAGGCAGCCACCCCAGGCCTTCACGGAAACCCGTTGACTCGGTGCCTC TAAGCAGAGGGAAGGAGCTGCTGCAGAAGGCTATCAGAAACCAGGGGAGCATCAGTGGAGGAGGCAGTGGGGGCAGCAGCAGCCTCCTGACCAATGCCCG CTCTTGGGGAGTGAGGATTCTGCACGTGGATGAAATGATGATGCACGTGCAACAGCTGTCTCTTGCGTCTTTATGTGTGAAAAAACAACAGCCAAAGAAG CCAGAGGGAACATGTCCAGCAGCAGAGTCAAGAACACGGAAAGTGGCCAGACTGAAGGCCCCGTTCCTCAAAATCGAAGATGAAAGCAGATGCAAGTCCC TTTGAGGCCCCGACGACCCTGGGCAGCATGCACCATACCAGAGAATCCAAGGATGGAGAGCCAAGCCCACGATCAGCTGCCCACACCATGCCCAGGAGGA AGAAAGGCTACTGCGAGTGCTGTCAGGAGGCCTTCGAGGAGCTCCATGTGCATCTTCAGAGTGCCCAGCACCGGAGCTTTGCCCTGGAAGCCCATCTATA TGCAGAAGTGGACAGGATCATTGCTCAGCTCAGCCACAGCTTTGCAGACATCCCTTTCCAGGCTGGCCTCCCCAGGTGGTCAGGTTCCCCAGCTTCTGAT TGTGACCCTCTCTGTCCTGAGACTCTGCACCCCCATCAGCCCTCCCATCCCAGGGCAGCATCTCCCAGGATAAGGAAAGAAGACAGCTGCCAGGCATCAG GCCATGAGTCCAGGCTCCTCGCCTCCCTCCAGCCCTTGTGCCACTCACAGACCTGCCTCTCTCTCCCAGACCCCTTCCCCTGGCAGCCCACAGACAGACC AGCAGAGTTCTGGGCCACACAGCCCTCCTGGCTGGGGAAAGGGTGGCCCCCAGGGCCTGAGGATTCTGAGTGTACAGCCACTGGCCCTGTATCCCAGGAG GCTGGCCAGCTCCTCAGCTGCCCCACAGCTCCAGGCTGGCCATCAGCTCCCCTGTACTCAGCTACCAGTGTGCAGCCCTCGGGAGCACCTGTAGAGAGCA GGTCTACCTCCCTCCTGCAGCCCCTGCCAGCCAGTGCGGGAGCCAGCTGTTCCCGATGCCTCTGGGCCCCCCAGCCTCTCCAGGTGCCCTGTCTCCCTGT CTCCCAGCCCTGGTCCCAACCCCAGCCCCAGCCCCAGCCCCATGCTGGCAGAGAGCTGCTCCTACGAGTCCCCAAGGTGCTTGGCTCTTCACAGGGCCAG GCAGCTCCAGATTGAAGGGCCACAGATACAACTTACTCCTCTGCTGGGTGCTGCACACCCGGCCAGGGCTGACCAGGACGCAGGAGGGGCAGAACCCCAG GACTTCCCCAAAAGAGCTAGAAGGAGGCCCGGGCCTGGCCTTTGAAGTCCAGCAAGCAGCTGTCACGCTCAGCTCCATGGAGCTGACAGAGCCACCCCTC CCACTGGCCAGGTCTTTGGGAGAGAATCTGGGCTGGGGGCTGCCCTCTCCTACCCCTGCCTCCTGCCATGTTCCTGACCAGGCAGAGTCTACAGTGGGCT TGCCCAGCTGAGGCTGGCCGCCCACGCCAGGAGGCAGAGGCGAAGTTGGCATGGGGAGGGACCACAAAGGAGCTGGGGCAGCAGGAGTCCTGGCCCGGCT TCCTGGCCCTCCACAGGCCCTTGCCTCTCTGCAGAGCCGCGGGTTAGCTGTTGGTGTGCTCCACCCCCAACCCCGGCCTCCTTTCTCACGAGCATGTGGC CGCTCCTGCTTTCCAAAATGACTGTGTTTGTCCCCTCCCCCAGCCCCCCACGCTCCCGCACACAGATCCTCAGGAACATATGAAGCAGAGGAGGGGCTTG GGCTGCACCACTCACAGAGCTCCCTCCCCCAGGCACTTAGTTGGGGCCCAGCACTGACCTTTCCCCTGAGCCCAGGATGTGGCCAGAGCCCCCTCTGGGA CCCCTCTCGCCCCTTCTCTGCCTCCTCAGCTTGAGCTGCCTGCCCGAAGTTCGGCTGTTCCGGGGCCAGTGTGTCACCTGCCAACTTCCACATCACCCTC CTCCCTCGCTCCCTCCTCTCCTTCCCCAAGGACCTCCCCCCATTTCTGGCAGCCAAGCCATTAATCTGGAGACAGAAATGGGTTTGCTATCGATTCTCTG GCCACTTTTTCTTTCATTACAATTTGTACCGTGATTCTTCTCACCCTTCTCTGCGTCCGTGCATTTAAAGAGTTGTCTCTTTAAATGTTGAAGCTTCCGG AAGCCTGATGCTATTCTGTGTCTCCTTTCAAAGGAAGAAGGGGGGGCCCAGCTATGGGTGAGGACTCAAGTTATTAGTTTGGAAATAGAGCAACTATGTG TACAGCCCACCTTAGAGGTCATGTTACCCCCTTCCTGTTAAATTTTACAATTAATTTTGGTTCAGGAAATGTAAATAAATTTGTTAATTACAAATAGCAA AGAGATGGGGCTGGAATGCCATATGTCGGTCCTTGATCTGCCCTCCAGTGACCCAAGGCAGGGCTGCGGGCCAGCAGCGATGGACAGAATCACTAGATGG TGTGATGGGACCTCCTGCAAGTCACACATGTGTGAGTGCCACAACCCTCCTGCCGGCCTTGCCCAAGGGCTCCAGGGAGCAGGGCTGCCTCTGTCCCTGC CCAGCCTCCTTTACCCAGTCTCATCTGGTCACTTCCTTGGCTCTGCTGCCTGGGGAGTGGTCGCCTGCAGAGGACATGCCCCTCCATCCCTCCCAAGAAA ACTCCTTTGCCCCGGCGGACATTCCTGTTAAGGGCCCACTCCTCTTCCCTGAAGCCAGACCGTGGCTTATGTCTGCACGCTGCTGGGTTCGTCCCTTTCC TTTTGTGACATGGGGTTGCCTCATTCCCCATGACACCACCCCTCTGCATGAGGAAGTTTCCCCTTGCCCCTGTCTCAGACTTGGATACCTTTACCTGCTG CTCACACAAAGCCTGTGGTGCCGGGTTCGGGTGCCCTCATTGTCAACTGCAGG

DRF1.F1 (forward primer)
DRF1.R1 (reverse primer)
Alternative acceptor

Frame 0

GCREGEKERRKGSLIAEARCGVVEGA*EVTSGR RLPRAGEFHG*E*APGPGPRSFQVSRKMPEELTR CQEASLFRKVLLLGSACWQESPVFDGGHSATGW GN*GFSEQRSKLHRVQPQRSKGREQWEKP*RLP *P*PQ*GQSGNIGHG*SKRQPPQAFTETR*LGAS KQREGAAAEGYQKPGEHQWRRQWGQQQPPDQCP LLGSEDSARG*NDDARATAVSCVFMCEKTTAKE ARGNMSSSRVKNTESGQTEGPVPQNRR*KQMQVP LRPRRPWAACTIPENPRMESQAHDQLPTPCPGG RKATASAVRRPSRSSMCIFRVPSTGALPWKPIY MQKWTGSLLSSATALQTSLSRLASPGGQVPQLLI VTLSVLRLCTPISPPIPGQHLPG*GKKTAARHQ AMSPGSSPPSSPCATHRPASLSQTPSPGSPQTD QQSSGPHSPPGWGKGGPQGLRILSVQPLALYPRR LASSSAAPQLQAGHQLPCTQLPVCSPREHL*RA GLPPSCSPCQPVREPAVPDASGPPSLSRCPVSL SPSPGPNPSPSPSPMLAESCSYESPRCLALHRAR QLQIEGPQIQLTPLLGAAHPARADQDAGGAEPQ DFPKRARRRPGPGL*SPASSCHAQLHGADRATP PTGQVFGRESGLGAALSYPCLLPCS*PGRVYSGL AQLRLAAHARRQRRSWHGEGPQRSWGSRSPGPA SWPSTGPCLSAEPRVSCWCAPPPTPASFLTSMW PLLLSKMTVFVPSPSPPRSRTQILRNI*SRGGAW AAPLTELPPPGT*LGPSTDLSPEPRMWPEPPLG PLSPLLCLLSLSCLPEVRLFRGQCVTCQLPHHP PPSLPPLLPQGPPPISGSQAINLETEMGLLSILW PLFLSLQFVP*FFSPFSASVHLKSCLFKC*SFR KPDAILCLLSKEEGGAQLWVRTQVISLEIEQLC VQPTLEVMLPPSC*ILQLILVQEM*INLLITNSK EMGLECHMSVLDLPSSDPRQGCGPAAMDRITRW CDGTSCKSHMCECHNPPAGLAQGLQGAGLPLSL PSLLYPVSSGHFLGSAAWGVVACRGHAPPSLPRK LLCPGGHSC*GPTPLP*SQTVAYVCTLLGSSLS FCDMGLPHSP*HHPSA*GSFPLPLSQTWIPLPA AHTKPVVPGSGALIVNCR

Frame 1

DAGKGRKKGGRGR**LRRGAESWRGLRK*PAGD DCLELESSMAESRLRAPDLGVSRCLGKCQKNSP GARKHPFSGKSFYLDLPAGKNLQFLTGAIQQLGG VIEGFLSKEVSYIVSSRREVKAESSGKSHRGCP SPSPSEVRVETSAMVDPKGSHPRPSRKPVDSVP LSRGKELLQKAIRNQGSISGGGSGGSSSLLTNAR SWGVRILHVDEMMMHVQQLSLASLCVKKQQPKK PEGTCPAAESRTRKVARLKAPFLKIEDESRCKS L*GPDDPGQHAPYQRIQGWRAKPTISCPHHAQEE ERLLRVLSGGLRGAPCASSECPAPELCPGSPSI CRSGQDHCSAQPQLCRHPFPGWPPQVVRFPSF* L*PSLS*DSAPPSALPSQGSISQDKERRQLPGIR P*VQAPRLPPALVPLTDLPLSPRPLPLAAHRQT SRVLGHTALLAGERVAPRA*GF*VYSHWPCIPG GWPAPQLPHSSRLAISSPVLSYQCAALGSTCREQ VYLPPAAPASQCGSQLFPMPLGPPASPGALSPC LPALVPTPAPAPAPCWQRAAPTSPQGAWLFTGP GSSRLKGHRYNLLLCWVLHTRPGLTRTQEGQNPR TSPKELEGGPGLAFEVQQAAVTLSSMELTEPPL PLARSLGENLGWGLPSPTPASCHVPDQAESTVG LPS*GWPPTPGGRGEVGMGRDHKGAGAAGVLARL PGPPQALASLQSRGLAVGVLHPQPRPPFSRACG RSCFPK*LCLSPPPAPHAPAHRSSGTYEAEEGL GLHHSQSSLPQALSWGPALTFPLSPGCGQSPLWD PSRPFSASSA*AACPKFGCSGASVSPANFHITL LPRSLLSFPKDLPPFLAAKPLIWRQKWVCYRFS GHFFFHYNLYRDSSHPSLRPCI*RVVSLNVEASG SLMLFCVSFQRKKGGPSYG*GLKLLVWK*SNYV YSPP*RSCYPLPVKFYN*FWFRKCK*IC*LQIA KRWGWNAICRSLICPPVTQGRAAGQQRWTESLDG VMGPPASHTCVSATTLLPALPKGSREQGCLCPC PASFTQSHLVTSLALLPGEWSPAEDMPLHPSQE NSFAPADIPVKGPLLFPEARPWLMSARCWVRPFP FVTWGCLIPHDTTPLHEEVSPCPCLRLGYLYLL LTQSLWCRVRVPSLSTA

Frame 2

MPGRGERKAEGVVNS*GEVRSRGGGLGSDQRET IASSWRVPWLRVGSGPRT*EFPGV*ENARRTHQ VPGSIPFPESPFTWICLLARISSF*RGPFSNWV G*LRVF*AKK*VTSCPAAEK*RQRAVGKAIEAAL ALAPVRSEWKHRPWLIQKAATPGLHGNPLTRCL *AEGRSCCRRLSETRGASVEEAVGAAAAS*PMP ALGE*GFCTWMK**CTCNSCLLRLYV*KNNSQRS QREHVQQQSQEHGKWPD*RPRSSKSKMKADASP FEAPTTLGSMHHTRESKDGEPSPRSAAHTMPRR KKGYCECCQEAFEELHVHLQSAQHRSFALEAHLY AEVDRIIAQLSHSFADIPFQAGLPRWSGSPASD CDPLCPETLHPHQPSHPRAASPRIRKEDSCQAS GHESRLLASLQPLCHSQTCLSLPDPFPWQPTDRP AEFWATQPSWLGKGWPPGPEDSECTATGPVSQE AGQLLSCPTAPGWPSAPLYSATSVQPSGAPVES RSTSLLQPLPASAGASCSRCLWAPQPLQVPCLPV SQPWSQPQPQPQPHAGRELLLRVPKVLGSSQGQ AAPD*RATDTTYSSAGCCTPGQG*PGRRRGRTP GLPQKS*KEARAWPLKSSKQLSRSAPWS*QSHPS HWPGLWERIWAGGCPLLPLPPAMFLTRQSLQWA CPAEAGRPRQEAEAKLAWGGTTKELGQQESWPG FLALHRPLPLCRAAG*LLVCSTPNPGLLSHEHVA APAFQNDCVCPLPQPPTLPHTDPQEHMKQRRGL GCTTHRAPSPRHLVGAQH*PFP*AQDVARAPSG TPLAPSLPPQLELPARSSAVPGPVCHLPTSTSPS SLAPSSPSPRTSPHFWQPSH*SGDRNGFAIDSL ATFSFITICTVILLTLLCVRAFKELSL*MLKLP EA*CYSVSPFKGRRGGPAMGEDSSY*FGNRATMC TAHLRGHVTPFLLNFTINFGSGNVNKFVNYK*Q RDGAGMPYVGP*SALQ*PKAGLRASSDGQNH*M V*WDLLQVTHV*VPQPSCRPCPRAPGSRAASVPA QPPLPSLIWSLPWLCCLGSGRLQRTCPSIPPKK TPLPRRTFLLRAHSSSLKPDRGLCLHAAGFVPF LL*HGVASFPMTPPLCMRKFPLAPVSDLDTFTCC SHKACGAGFGCPHCQLQ

Sequence DRF1.aAug05

GTAGGGCGGATCTCGCAGCTACGGCGTTCTTTTAGGGGTGGAGCCGGCAGGAAATTTAAACTGAAGCCGCGGCCGAAAACGCCAAGAGATTGATGCTGTA GCTGCCCTGAGATAACCAGGACTGTGGAATCGGGAAGAGCTCATGGAGCTCGCGAATGTAATACGGAGGCCTCTGAGGAAGGAGTACGGAGGCCGAGAAG GAGCCGGCATTTGATGAGCGAACCGGGAAAGGGAGACGATTGCCTCGAGCTGGAGAGTTCCATGGCTGAGAGTAGGCTCCGGGCCCCGGACCTAGGAGTT TCCAGGTGTCTAGGAAAATGCCAGAAGAACTCACCAGGTGCCAGGAAGCATCCCTTTTCCGGAAAGTCCTTTTACTTGGATCTGCCTGCTGGCAAGAATC TCCAGTTTTTGACGGGGGCCATTCAGCAACTGGGTGGGGTAATTGAGGGTTTTCTGAGCAAAGAAGTAAGTTACATCGTGTCCAGCCGCAGAGAAGTAAA GGCAGAGAGCAGTGGGAAAAGCCATAGAGGCTGCCCTAGCCCTAGCCCCAGTGAGGTCAGAGTGGAAACATCGGCCATGGTTGATCCAAAAGGCAGCCAC CCCAGGCCTTCACGGAAACCCGTTGACTCGGTGCCTCTAAGCAGAGGGAAGGAGCTGCTGCAGAAGGCTATCAGAAACCAGGGGAGCATCAGTGGAGGAG GCAGTGGGGGCAGCAGCAGCCTCCTGACCAATGCCCGCTCTTGGGGAGTGAGGATTCTGCACGTGGATGAAATGATGATGCACGTGCAACAGCTGTCTCT TGCGTCTTTATGTGTGAAAAAACAACAGCCAAAGAAGCCAGAGGGAACATGTCCAGCAGCAGAGTCAAGAACACGGAAAGTGGCCAGACTGAAGGCCCCG TTCCTCAAAATCGAAGATGAAAGCAGGAAGTTTCGTCCTTTCCATCATCAGTTTAAATCCTTTCCTGAAATTTCTTTTCTTGGACCCAAAGATGCAAGTC CCTTTGAGGCCCCGACGACCCTGGGCAGCATGCACCATACCAGAGAATCCAAGGATGGAGAGCCAAGCCCACGATCAGCTGCCCACACCATGCCCAGGAG GAAGAAAGGCTACTGCGAGTGCTGTCAGGAGGCCTTCGAGGAGCTCCATGTGCATCTTCAGAGTGCCCAGCACCGGAGCTTTGCCCTGGAAGCCCATCTA TATGCAGAAGTGGACAGGATCATTGCTCAGCTCAGCCACAGCTTTGCAGACATCCCTTTCCAGGCTGGCCTCCCCAGGTGGTCAGGTTCCCCAGCTTCTG ATTGTGACCCTCTCTGTCCTGAGACTCTGCACCCCCATCAGCCCTCCCATCCCAGGGCAGCATCTCCCAGGATAAGGAAAGAAGACAGCTGCCAGGCATC AGTGACCCAAGGCAGGGCTGCGGGCCAGCAGCGATGGACAGAATCACTAGATGGTGTGATGGGACCTCCTGCAAGTCACACATGTGTGAGTGCCACAACC CTCCTGCCGGCCTTGCCCAAGGGCTCCAGGGAGCAGGGCTGCCTCTGTCCCTGCCCAGCCTCCTTTACCCAGTCTCATCTGGTCACTTCCTTGGCTCTGC TGCCTGGGGAGTGGTCGCCTGCAGAGGACATGCCCCTCCATCCCTCCCAAGAAAACTCCTTTGCCCCGGCGGACATTCCTGTTAAGGGCCCACTCCTCTT CCCTGAAGCCAGACCGTGGCTTATGTCTGCACGCTGCTGGGTTCGTCCCTTTCCTTTTGTGACATGGGGTTGCCTCATTCCCCATGACACCACCCCTCTG CATGAGGAAGTTTCCCCTTGCCCCTGTCTCAGACTTGGATACCTTTACCTGCTGCTCACACAAAGCCTGTGGTGCCGGGTTCGGGTGCCCTCATTGTCAA CTGCAGGACCCATTCCCCGAACCTCACATCCGTGTACCCTTGCCTTCCCCTCCTATCTCAATGATCATGACCTTGGACATCTCTGCCAGGCCAAACCCCA AGGCTGGAACACTCCTCAGCCATTTCTCCATTGCGGCTTCCTGGCTGTAGACTCAGGTTAGAGGTGAACCCAGAACACCTGAGACTTGACCCAGGATGGA TGGGTGCTGCTTGATGTGAATGAGGTCCCGCAGTGGCTCCTTGGCGTGAGCACTGCTCAGACTCCTTTCCACTCCAGCCCCCTTTCCACATCGCACCAGA TGACTTTTACCCAGACCCAGTGGGCATTGCCTTATCTTGCAGTCAGTCCCTTTTCAACATGTTGCCGTTTCTTTCTGAAGAGGTGTCCTCCCTCCACAAG TCACACTGTCTGTCCCTGGCCCTCCAGCCCACCTCGCCAACCACTCTTGTTGGTTTCCTTCTCAGACTTGCCACCTTTCCCCTCTGCCCCAAAATGCCAT GCTCCTCTCCTGGAAAACACTTGAGTTGATTCAGTAAATCGACTTCAAATACTTGAAGGCTCCCACCTTCTGTTCTCTGGCTCCTTCCTGCGGTCTATAC CTACCGCCTCCTCTTCACCTCCTTCCCTTCCACACTTCCTTCCTGGGTAGCTCTGCCTGAAGCATTCCACTAAGATCATCTATTCCAAGGTCATGGACAG GCTACTGGTGACCAAAGTTGGTTCCTTTTCTCCTTTCTTTCCTCCTTGAAGCCTGGCTCCCTTGGTCGCAGCAGCCCCTCAGTGGCCTGGTTCTCCTGTC CCCCTGCCCTTCCTCACCATTGCCCATTCCCTCGTTCGTTCATTCAGCACAGGCCTTGCCGTCTGCCCTGAGTCAGCTCCGAGACACCTGAAGAGCCCTC CAGCCCTAACTACTTTACTCAGACTAGGTCCCCAGGCCTTTGTTCTTGCCTCTTCTCGCTGAGCCTTTCACTTCTCGGCAGATGTGACCGATTGGTAGCT CCACCCCAACTCCCTTCTGCTGGGTGGAATGCAGGAGCTAGCTGCCTCCAACTCACTGTGACCTCAGAAAAATGCCTTTATTACTCGGGCCTCAGTTTCC TCGTCTTTAAGTAAGGGGCTTGGATGAGATGATTTCAGGACCCTTTCCAATAATAAAATACTGTGACTGCCAGCAA

DRF1.F1 (forward primer)
DRF1.R1 (reverse primer)
Alternative acceptor

Frame 0

VGRISQLRRSFRGGAGRKFKLKPRPKTPRD*CC SCPEITRTVESGRAHGARECNTEASEEGVRRPRR SRHLMSEPGKGDDCLELESSMAESRLRAPDLGV SRCLGKCQKNSPGARKHPFSGKSFYLDLPAGKN LQFLTGAIQQLGGVIEGFLSKEVSYIVSSRREVK AESSGKSHRGCPSPSPSEVRVETSAMVDPKGSH PRPSRKPVDSVPLSRGKELLQKAIRNQGSISGG GSGGSSSLLTNARSWGVRILHVDEMMMHVQQLSL ASLCVKKQQPKKPEGTCPAAESRTRKVARLKAP FLKIEDESRKFRPFHHQFKSFPEISFLGPKDAS PFEAPTTLGSMHHTRESKDGEPSPRSAAHTMPRR KKGYCECCQEAFEELHVHLQSAQHRSFALEAHL YAEVDRIIAQLSHSFADIPFQAGLPRWSGSPAS DCDPLCPETLHPHQPSHPRAASPRIRKEDSCQAS VTQGRAAGQQRWTESLDGVMGPPASHTCVSATT LLPALPKGSREQGCLCPCPASFTQSHLVTSLAL LPGEWSPAEDMPLHPSQENSFAPADIPVKGPLLF PEARPWLMSARCWVRPFPFVTWGCLIPHDTTPL HEEVSPCPCLRLGYLYLLLTQSLWCRVRVPSLS TAGPIPRTSHPCTLAFPSYLNDHDLGHLCQAKPQ GWNTPQPFLHCGFLAVDSG*R*TQNT*DLTQDG WVLLDVNEVPQWLLGVSTAQTPFHSSPLSTSHQ MTFTQTQWALPYLAVSPFSTCCRFFLKRCPPSTS HTVCPWPSSPPRQPLLLVSFSDLPPFPSAPKCH APLLENT*VDSVNRLQILEGSHLLFSGSFLRSI PTASSSPPSLPHFLPG*LCLKHSTKIIYSKVMDR LLVTKVGSFSPFFPP*SLAPLVAAAPQWPGSPV PLPFLTIAHSLVRSFSTGLAVCPESAPRHLKSP PALTTLLRLGPQAFVLASSR*AFHFSADVTDW*L HPNSLLLGGMQELAASNSL*PQKNAFITRASVS SSLSKGLG*DDFRTLSNNKIL*LPA

Frame 1

*GGSRSYGVLLGVEPAGNLN*SRGRKRQEIDAV AALR*PGLWNREELMELANVIRRPLRKEYGGRE GAGI**ANRERETIASSWRVPWLRVGSGPRT*EF PGV*ENARRTHQVPGSIPFPESPFTWICLLARI SSF*RGPFSNWVG*LRVF*AKK*VTSCPAAEK* RQRAVGKAIEAALALAPVRSEWKHRPWLIQKAAT PGLHGNPLTRCL*AEGRSCCRRLSETRGASVEE AVGAAAAS*PMPALGE*GFCTWMK**CTCNSCL LRLYV*KNNSQRSQREHVQQQSQEHGKWPD*RPR SSKSKMKAGSFVLSIISLNPFLKFLFLDPKMQV PLRPRRPWAACTIPENPRMESQAHDQLPTPCPG GRKATASAVRRPSRSSMCIFRVPSTGALPWKPIY MQKWTGSLLSSATALQTSLSRLASPGGQVPQLL IVTLSVLRLCTPISPPIPGQHLPG*GKKTAARH Q*PKAGLRASSDGQNH*MV*WDLLQVTHV*VPQP SCRPCPRAPGSRAASVPAQPPLPSLIWSLPWLC CLGSGRLQRTCPSIPPKKTPLPRRTFLLRAHSS SLKPDRGLCLHAAGFVPFLL*HGVASFPMTPPLC MRKFPLAPVSDLDTFTCCSHKACGAGFGCPHCQ LQDPFPEPHIRVPLPSPPISMIMTLDISARPNP KAGTLLSHFSIAASWL*TQVRGEPRTPET*PRMD GCCLM*MRSRSGSLA*ALLRLLSTPAPFPHRTR *LLPRPSGHCLILQSVPFQHVAVSF*RGVLPPQ VTLSVPGPPAHLANHSCWFPSQTCHLSPLPQNAM LLSWKTLELIQ*IDFKYLKAPTFCSLAPSCGLY LPPPLHLLPFHTSFLGSSA*SIPLRSSIPRSWT GYW*PKLVPFLLSFLLEAWLPWSQQPLSGLVLLS PCPSSPLPIPSFVHSAQALPSALSQLRDT*RAL QP*LLYSD*VPRPLFLPLLAEPFTSRQM*PIGS STPTPFCWVECRS*LPPTHCDLRKMPLLLGPQFP RL*VRGLDEMISGPFPIIKYCDCQQ

Frame 2

RADLAATAFF*GWSRQEI*TEAAAENAKRLML* LP*DNQDCGIGKSSWSSRM*YGGL*GRSTEAEK EPAFDERTGKGRRLPRAGEFHG*E*APGPGPRS FQVSRKMPEELTRCQEASLFRKVLLLGSACWQES PVFDGGHSATGWGN*GFSEQRSKLHRVQPQRSK GREQWEKP*RLP*P*PQ*GQSGNIGHG*SKRQP PQAFTETR*LGASKQREGAAAEGYQKPGEHQWRR QWGQQQPPDQCPLLGSEDSARG*NDDARATAVS CVFMCEKTTAKEARGNMSSSRVKNTESGQTEGP VPQNRR*KQEVSSFPSSV*ILS*NFFSWTQRCKS L*GPDDPGQHAPYQRIQGWRAKPTISCPHHAQE EERLLRVLSGGLRGAPCASSECPAPELCPGSPS ICRSGQDHCSAQPQLCRHPFPGWPPQVVRFPSF* L*PSLS*DSAPPSALPSQGSISQDKERRQLPGI SDPRQGCGPAAMDRITRWCDGTSCKSHMCECHN PPAGLAQGLQGAGLPLSLPSLLYPVSSGHFLGSA AWGVVACRGHAPPSLPRKLLCPGGHSC*GPTPL P*SQTVAYVCTLLGSSLSFCDMGLPHSP*HHPS A*GSFPLPLSQTWIPLPAAHTKPVVPGSGALIVN CRTHSPNLTSVYPCLPLLSQ*S*PWTSLPGQTP RLEHSSAISPLRLPGCRLRLEVNPEHLRLDPGW MGAA*CE*GPAVAPWREHCSDSFPLQPPFHIAPD DFYPDPVGIALSCSQSLFNMLPFLSEEVSSLHK SHCLSLALQPTSPTTLVGFLLRLATFPLCPKMP CSSPGKHLS*FSKSTSNT*RLPPSVLWLLPAVYT YRLLFTSFPSTLPSWVALPEAFH*DHLFQGHGQ ATGDQSWFLFSFLSSLKPGSLGRSSPSVAWFSC PPALPHHCPFPRSFIQHRPCRLP*VSSETPEEPS SPNYFTQTRSPGLCSCLFSLSLSLLGRCDRLVA PPQLPSAGWNAGASCLQLTVTSEKCLYYSGLSF LVFK*GAWMR*FQDPFQ**NTVTAS

Sequence DRF1.bAug05

GTAGGGCGGATCTCGCAGCTACGGCGTTCTTTTAGGGGTGGAGCCGGCAGGAAATTTAAACTGAAGCCGCGGCCGAAAACGCCAAGAGATTGATGCTGTA GCTGCCCTGAGATAACCAGGACTGTGGAATCGGGAAGAGCTCATGGAGCTCGCGAATGTAATACGGAGGCCTCTGAGGAAGGAGTACGGAGGCCGAGAAG GAGCCGGCATTTGATGAGCGAACCGGGAAAGGGAGACGATTGCCTCGAGCTGGAGAGTTCCATGGCTGAGAGTAGGCTCCGGGCCCCGGACCTAGGAGTT TCCAGGTGTCTAGGAAAATGCCAGAAGAACTCACCAGGTGCCAGGAAGCATCCCTTTTCCGGAAAGTCCTTTTACTTGGATCTGCCTGCTGGCAAGAATC TCCAGTTTTTGACGGGGGCCATTCAGCAACTGGGTGGGGTAATTGAGGGTTTTCTGAGCAAAGAAGTAAGTTACATCGTGTCCAGCCGCAGAGAAGTAAA GGCAGAGAGCAGTGGGAAAAGCCATAGAGGCTGCCCTAGCCCTAGCCCCAGTGAGGTCAGAGTGGAAACATCGGCCATGGTTGATCCAAAAGGCAGCCAC CCCAGGCCTTCACGGAAACCCGTTGACTCGGTGCCTCTAAGCAGAGGGAAGGAGCTGCTGCAGAAGGCTATCAGAAACCAGAAATGATGATGCACGTGCA ACAGCTGTCTCTTGCGTCTTTATGTGTGAAAAAACAACAGCCAAAGAAGCCAGAGGGAACATGTCCAGCAGCAGAGTCAAGAACACGGAAAGTGGCCAGA CTGAAGGCCCCGTTCCTCAAAATCGAAGATGAAAGCAGGAAGTTTCGTCCTTTCCATCATCAGTTTAAATCCTTTCCTGAAATTTCTTTTCTTGGACCCA AAGATGCAAGTCCCTTTGAGGCCCCGACGACCCTGGGCAGCATGCACCATACCAGAGAATCCAAGGATGGAGAGCCAAGCCCACGATCAGCTGCCCACAC CATGCCCAGGAGGAAGAAAGGCTACTGCGAGTGCTGTCAGGAGGCCTTCGAGGAGCTCCATGTGCATCTTCAGAGTGCCCAGCACCGGAGCTTTGCCCTG GAAGCCCATCTATATGCAGAAGTGGACAGGATCATTGCTCAGCTCAGCCACAGCTTTGCAGACATCCCTTTCCAGGCTGGCCTCCCCAGGTGGTCAGGTT CCCCAGCTTCTGATTGTGACCCTCTCTGTCCTGAGACTCTGCACCCCCATCAGCCCTCCCATCCCAGGGCAGCATCTCCCAGGATAAGGAAAGAAGACAG CTGCCAGGCATCAGGTATCCCAGAGCAGGATGGGACAGTGGACAGCACGCAGGCACCAGCTGAACGTGCAGGGACTGGTGAGGTACCTGGACCTATAGCA AGCTGCCAGGACCTGGGGGTGTCAGTTGATGTGTTTGTGGACCCCCCAGGGATACCAGTGTCCAGGAGCCCTGCTTGCCAATGCCTCCTGCCTAGCTCTG GTTTTATGGAACTCTCCTCTGGCCCAGACCTGGCACTTTTTGGCCACAAGCGGAAGGTTCAGTTCCCCAGTGGCAGTGCTAAGAAGAGAGTGGGGGCCTC CTGGCCACAGGCTTCATTTTTTGTCCCAATAGCCCCCAACCCCTGCGGCACCAGGACAACTAGTGGCAAGCGTCTTCCCTCTCTTCCCCTTACAGGCCAT GAGTCCAGGCTCCTCGCCTCCCTCCAGCCCTTGTGCCACTCACAGACCTGCCTCTCTCTCCCAGACCCCTTCCCCTGGCAGCCCACAGACAGACCAGCAG AGTTCTGGGCCACACAGCCCTCCTGGCTGGGGAAAGGGTGGCCCCCAGGGCCTGAGGATTCTGAGTGTACAGCCACTGGCCCTGTATCCCAGGAGGCTGG CCAGCTCCTCAGCTGCCCCACAGCTCCAGGCTGGCCATCAGCTCCCCTGTACTCAGCTACCAGTGTGCAGCCCTCGGGAGCACCTGTAGAGAGCAGGTCT ACCTCCCTCCTGCAGCCCCTGCCAGCCAGTGCGGGAGCCAGCTGTTCCCGATGCCTCTGGGCCCCCCAGCCTCTCCAGGTGCCCTGTCTCCCTGTCTCCC AGCCCTGGTCCCAACCCCAGCCCCAGCCCCAGCCCCATGCTGGCAGAGAGCTGCTCCTACGAGTCCCCAAGGTGCTTGGCTCTTCACAGGGCCAGGCAGC TCCAGATTGAAGGGCCACAGATACAACTTACTCCTCTGCTGGGTGCTGCACACCCGGCCAGGGCTGACCAGGACGCAGGAGGGGCAGAACCCCAGGACTT CCCCAAAAGAGCTAGAAGGAGGCCCGGGCCTGGCCTTTGAAGTCCAGCAAGCAGCTGTCACGCTCAGCTCCATGGAGCTGACAGAGCCACCCCTCCCACT GGCCAGGTCTTTGGGAGAGAATCTGGGCTGGGGGCTGCCCTCTCCTACCCCTGCCTCCTGCCATGTTCCTGACCAGGCAGAGTCTACAGTGGGCTTGCCC AGCTGAGGCTGGCCGCCCACGCCAGGAGGCAGAGGCGAAGTTGGCATGGGGAGGGACCACAAAGGAGCTGGGGCAGCAGGAGTCCTGGCCCGGCTTCCTG GCCCTCCACAGGCCCTTGCCTCTCTGCAGAGCCGCGGGTTAGCTGTTGGTGTGCTCCACCCCCAACCCCGGCCTCCTTTCTCACGAGCATGTGGCCGCTC CTGCTTTCCAAAATGACTGTGTTTGTCCCCTCCCCCAGCCCCCCACGCTCCCGCACACAGATCCTCAGGAACATATGAAGCAGAGGAGGGGCTTGGGCTG CACCACTCACAGAGCTCCCTCCCCCAGGCACTTAGTTGGGGCCCAGCACTGACCTTTCCCCTGAGCCCAGGATGTGGCCAGAGCCCCCTCTGGGACCCCT CTCGCCCCTTCTCTGCCTCCTCAGCTTGAGCTGCCTGCCCGAAGTTCGGCTGTTCCGGGGCCAGTGTGTCACCTGCCAACTTCCACATCACCCTCCTCCC TCGCTCCCTCCTCTCCTTCCCCAAGGACCTCCCCCCATTTCTGGCAGCCAAGCCATTAATCTGGAGACAGAAATGGGTTTGCTATCGATTCTCTGGCCAC TTTTTCTTTCATTACAATTTGTACCGTGATTCTTCTCACCCTTCTCTGCGTCCGTGCATTTAAAGAGTTGTCTCTTTAAATGTTGAAGCTTCCGGAAGCC TGATGCTATTCTGTGTCTCCTTTCAAAGGAAGAAGGGGGGGCCCAGCTATGGGTGAGGACTCAAGTTATTAGTTTGGAAATAGAGCAACTATGTGTACAG CCCACCTTAGAGGTCATGTTACCCCCTTCCTGTTAAATTTTACAATTAATTTTGGTTCAGGAAATGTAAATAAATTTGTTAATTACAAATAGCAAAGAGA TGGGGCTGGAATGCCATATGTCGGTCCTTGATCTGCCCTCCAGTGACCCAAGGCAGGGCTGCGGGCCAGCAGCGATGGACAGAATCACTAGATGGTGTGA TGGGACCTCCTGCAAGTCACACATGTGTGAGTGCCACAACCCTCCTGCCGGCCTTGCCCAAGGGCTCCAGGGAGCAGGGCTGCCTCTGTCCCTGCCCAGC CTCCTTTACCCAGTCTCATCTGGTCACTTCCTTGGCTCTGCTGCCTGGGGAGTGGTCGCCTGCAGAGGACATGCCCCTCCATCCCTCCCAAGAAAACTCC TTTGCCCCGGCGGACATTCCTGTTAAGGGCCCACTCCTCTTCCCTGAAGCCAGACCGTGGCTTATGTCTGCACGCTGCTGGGTTCGTCCCTTTCCTTTTG TGACATGGGGTTGCCTCATTCCCCATGACACCACCCCTCTGCATGAGGAAGTTTCCCCTTGCCCCTGTCTCAGACTTGGATACCTTTACCTGCTGCTCAC ACAAAGCCTGTGGTGCCGGGTTCGGGTGCCCTCATTGTCAACTGCAGG

DRF1.F1 (forward primer)
DRF1.R1 (reverse primer)
Alternative acceptor

Frame 0

VGRISQLRRSFRGGAGRKFKLKPRPKTPRD*CC SCPEITRTVESGRAHGARECNTEASEEGVRRPRR SRHLMSEPGKGDDCLELESSMAESRLRAPDLGV SRCLGKCQKNSPGARKHPFSGKSFYLDLPAGKN LQFLTGAIQQLGGVIEGFLSKEVSYIVSSRREVK AESSGKSHRGCPSPSPSEVRVETSAMVDPKGSH PRPSRKPVDSVPLSRGKELLQKAIRNQK**CTC NSCLLRLYV*KNNSQRSQREHVQQQSQEHGKWPD *RPRSSKSKMKAGSFVLSIISLNPFLKFLFLDP KMQVPLRPRRPWAACTIPENPRMESQAHDQLPT PCPGGRKATASAVRRPSRSSMCIFRVPSTGALPW KPIYMQKWTGSLLSSATALQTSLSRLASPGGQV PQLLIVTLSVLRLCTPISPPIPGQHLPG*GKKT AARHQVSQSRMGQWTARRHQLNVQGLVRYLDL*Q AARTWGCQLMCLWTPQGYQCPGALLANASCLAL VLWNSPLAQTWHFLATSGRFSSPVAVLRREWGP PGHRLHFLSQ*PPTPAAPGQLVASVFPLFPLQAM SPGSSPPSSPCATHRPASLSQTPSPGSPQTDQQ SSGPHSPPGWGKGGPQGLRILSVQPLALYPRRL ASSSAAPQLQAGHQLPCTQLPVCSPREHL*RAGL PPSCSPCQPVREPAVPDASGPPSLSRCPVSLSP SPGPNPSPSPSPMLAESCSYESPRCLALHRARQ LQIEGPQIQLTPLLGAAHPARADQDAGGAEPQDF PKRARRRPGPGL*SPASSCHAQLHGADRATPPT GQVFGRESGLGAALSYPCLLPCS*PGRVYSGLA QLRLAAHARRQRRSWHGEGPQRSWGSRSPGPASW PSTGPCLSAEPRVSCWCAPPPTPASFLTSMWPL LLSKMTVFVPSPSPPRSRTQILRNI*SRGGAWA APLTELPPPGT*LGPSTDLSPEPRMWPEPPLGPL SPLLCLLSLSCLPEVRLFRGQCVTCQLPHHPPP SLPPLLPQGPPPISGSQAINLETEMGLLSILWP LFLSLQFVP*FFSPFSASVHLKSCLFKC*SFRKP DAILCLLSKEEGGAQLWVRTQVISLEIEQLCVQ PTLEVMLPPSC*ILQLILVQEM*INLLITNSKE MGLECHMSVLDLPSSDPRQGCGPAAMDRITRWCD GTSCKSHMCECHNPPAGLAQGLQGAGLPLSLPS LLYPVSSGHFLGSAAWGVVACRGHAPPSLPRKL LCPGGHSC*GPTPLP*SQTVAYVCTLLGSSLSFC DMGLPHSP*HHPSA*GSFPLPLSQTWIPLPAAH TKPVVPGSGALIVNCR

Frame 1

*GGSRSYGVLLGVEPAGNLN*SRGRKRQEIDAV AALR*PGLWNREELMELANVIRRPLRKEYGGRE GAGI**ANRERETIASSWRVPWLRVGSGPRT*EF PGV*ENARRTHQVPGSIPFPESPFTWICLLARI SSF*RGPFSNWVG*LRVF*AKK*VTSCPAAEK* RQRAVGKAIEAALALAPVRSEWKHRPWLIQKAAT PGLHGNPLTRCL*AEGRSCCRRLSETRNDDARA TAVSCVFMCEKTTAKEARGNMSSSRVKNTESGQ TEGPVPQNRR*KQEVSSFPSSV*ILS*NFFSWTQ RCKSL*GPDDPGQHAPYQRIQGWRAKPTISCPH HAQEEERLLRVLSGGLRGAPCASSECPAPELCP GSPSICRSGQDHCSAQPQLCRHPFPGWPPQVVRF PSF*L*PSLS*DSAPPSALPSQGSISQDKERRQ LPGIRYPRAGWDSGQHAGTS*TCRDW*GTWTYS KLPGPGGVS*CVCGPPRDTSVQEPCLPMPPA*LW FYGTLLWPRPGTFWPQAEGSVPQWQC*EESGGL LATGFIFCPNSPQPLRHQDN*WQASSLSSPYRP *VQAPRLPPALVPLTDLPLSPRPLPLAAHRQTSR VLGHTALLAGERVAPRA*GF*VYSHWPCIPGGW PAPQLPHSSRLAISSPVLSYQCAALGSTCREQV YLPPAAPASQCGSQLFPMPLGPPASPGALSPCLP ALVPTPAPAPAPCWQRAAPTSPQGAWLFTGPGS SRLKGHRYNLLLCWVLHTRPGLTRTQEGQNPRT SPKELEGGPGLAFEVQQAAVTLSSMELTEPPLPL ARSLGENLGWGLPSPTPASCHVPDQAESTVGLP S*GWPPTPGGRGEVGMGRDHKGAGAAGVLARLP GPPQALASLQSRGLAVGVLHPQPRPPFSRACGRS CFPK*LCLSPPPAPHAPAHRSSGTYEAEEGLGL HHSQSSLPQALSWGPALTFPLSPGCGQSPLWDP SRPFSASSA*AACPKFGCSGASVSPANFHITLLP RSLLSFPKDLPPFLAAKPLIWRQKWVCYRFSGH FFFHYNLYRDSSHPSLRPCI*RVVSLNVEASGS LMLFCVSFQRKKGGPSYG*GLKLLVWK*SNYVYS PP*RSCYPLPVKFYN*FWFRKCK*IC*LQIAKR WGWNAICRSLICPPVTQGRAAGQQRWTESLDGV MGPPASHTCVSATTLLPALPKGSREQGCLCPCPA SFTQSHLVTSLALLPGEWSPAEDMPLHPSQENS FAPADIPVKGPLLFPEARPWLMSARCWVRPFPF VTWGCLIPHDTTPLHEEVSPCPCLRLGYLYLLLT QSLWCRVRVPSLSTA

Frame 2

RADLAATAFF*GWSRQEI*TEAAAENAKRLML* LP*DNQDCGIGKSSWSSRM*YGGL*GRSTEAEK EPAFDERTGKGRRLPRAGEFHG*E*APGPGPRS FQVSRKMPEELTRCQEASLFRKVLLLGSACWQES PVFDGGHSATGWGN*GFSEQRSKLHRVQPQRSK GREQWEKP*RLP*P*PQ*GQSGNIGHG*SKRQP PQAFTETR*LGASKQREGAAAEGYQKPEMMMHVQ QLSLASLCVKKQQPKKPEGTCPAAESRTRKVAR LKAPFLKIEDESRKFRPFHHQFKSFPEISFLGP KDASPFEAPTTLGSMHHTRESKDGEPSPRSAAHT MPRRKKGYCECCQEAFEELHVHLQSAQHRSFAL EAHLYAEVDRIIAQLSHSFADIPFQAGLPRWSG SPASDCDPLCPETLHPHQPSHPRAASPRIRKEDS CQASGIPEQDGTVDSTQAPAERAGTGEVPGPIA SCQDLGVSVDVFVDPPGIPVSRSPACQCLLPSS GFMELSSGPDLALFGHKRKVQFPSGSAKKRVGAS WPQASFFVPIAPNPCGTRTTSGKRLPSLPLTGH ESRLLASLQPLCHSQTCLSLPDPFPWQPTDRPA EFWATQPSWLGKGWPPGPEDSECTATGPVSQEAG QLLSCPTAPGWPSAPLYSATSVQPSGAPVESRS TSLLQPLPASAGASCSRCLWAPQPLQVPCLPVS QPWSQPQPQPQPHAGRELLLRVPKVLGSSQGQAA PD*RATDTTYSSAGCCTPGQG*PGRRRGRTPGL PQKS*KEARAWPLKSSKQLSRSAPWS*QSHPSH WPGLWERIWAGGCPLLPLPPAMFLTRQSLQWACP AEAGRPRQEAEAKLAWGGTTKELGQQESWPGFL ALHRPLPLCRAAG*LLVCSTPNPGLLSHEHVAA PAFQNDCVCPLPQPPTLPHTDPQEHMKQRRGLGC TTHRAPSPRHLVGAQH*PFP*AQDVARAPSGTP LAPSLPPQLELPARSSAVPGPVCHLPTSTSPSS LAPSSPSPRTSPHFWQPSH*SGDRNGFAIDSLAT FSFITICTVILLTLLCVRAFKELSL*MLKLPEA *CYSVSPFKGRRGGPAMGEDSSY*FGNRATMCT AHLRGHVTPFLLNFTINFGSGNVNKFVNYK*QRD GAGMPYVGP*SALQ*PKAGLRASSDGQNH*MV* WDLLQVTHV*VPQPSCRPCPRAPGSRAASVPAQ PPLPSLIWSLPWLCCLGSGRLQRTCPSIPPKKTP LPRRTFLLRAHSSSLKPDRGLCLHAAGFVPFLL *HGVASFPMTPPLCMRKFPLAPVSDLDTFTCCS HKACGAGFGCPHCQLQ

Sequence DRF1.cAug05

CTTGATTTCCTTCCCTGCCTTTCTCTGTTAGAGGAGTGCTAGCATGTTTAGCCCTTGCTGGTCTTTCACCTGAGGGTACAGCTGAGTAGTTCACACAGCA TGGCCTCCCTCACAGCCACAGCTCTTCAGTGGTGCCTTAAAGTCCTTAGCAAGCCATGAGGCACCTTCTGTTCAAAGATGAGTTGTATTCAATGCTGCAA AAGAGCATGACACCCGCTCAGGTGCTGTTCCAGGGATAGAGGACCCAGTCCTTAACCTCCGCTGTCAGGAGATGCAGAGGGGATGTGTAGCCAGAAGGTG GCAGGCAAGGCTGGAGTGATTCAGTGGGCTGGCCCAGCGAGTTTAGTCCTGCAGCCCAGGAAGGGGCTGTGCTAGGTAAGTGAAGACTGGAAGCCTTTGG CACAAACCCTCTGTCTTCTCCACAGAAATGATGATGCACGTGCAACAGCTGTCTCTTGCGTCTTTATGTGTGAAAAAACAACAGCCAAAGAAGCCAGAGG GAACATGTCCAGCAGCAGAGTCAAGAACACGGAAAGTGGCCAGACTGAAGGCCCCGTTCCTCAAAATCGAAGATGAAAGCAGGAAGTTTCGTCCTTTCCA TCATCAGTTTAAATCCTTTCCTGAAATTTCTTTTCTTGGACCCAAAGATGCAAGTCCCTTTGAGGCCCCGACGACCCTGGGCAGCATGCACCATACCAGA GAATCCAAGGATGGAGAGCCAAGCCCACGATCAGCTGCCCACACCATGCCCAGGAGGAAGAAAGGCTACTGCGAGTGCTGTCAGGAGGCCTTCGAGGAGC TCCATGTGCATCTTCAGAGTGCCCAGCACCGGAGCTTTGCCCTGGAAGCCCATCTATATGCAGAAGTGGACAGGATCATTGCTCAGCTCAGCCACAGCTT TGCAGACATCCCTTTCCAGGCTGGCCTCCCCAGGTGGTCAGGTTCCCCAGCTTCTGATTGTGACCCTCTCTGTCCTGAGACTCTGCACCCCCATCAGCCC TCCCATCCCAGGGCAGCATCTCCCAGGATAAGGAAAGAAGACAGCTGCCAGGCATCAGGTATCCCAGAGCAGGATGGGACAGTGGACAGCACGCAGGCAC CAGCTGAACGTGCAGGGACTGGTGAGGTACCTGGACCTATAGCAAGCTGCCAGGACCTGGGGGTGTCAGTTGATGTGTTTGTGGACCCCCCAGGGATACC AGTGTCCAGGAGCCCTGCTTGCCAATGCCTCCTGCCTAGCTCTGGTTTTATGGAACTCTCCTCTGGCCCAGACCTGGCACTTTTTGGCCACAAGCGGAAG GTTCAGTTCCCCAGTGGCAGTGCTAAGAAGAGAGTGGGGGCCTCCTGGCCACAGGCTTCATTTTTTGTCCCAATAGCCCCCAACCCCTGCGGCACCAGGA CAACTAGTGGCAAGCGTCTTCCCTCTCTTCCCCTTACAGGCCATGAGTCCAGGCTCCTCGCCTCCCTCCAGCCCTTGTGCCACTCACAGACCTGCCTCTC TCTCCCAGACCCCTTCCCCTGGCAGCCCACAGACAGACCAGCAGAGTTCTGGGCCACACAGCCCTCCTGGCTGGGGAAAGGGTGGCCCCCAGGGCCTGAG GATTCTGAGTGTACAGCCACTGGCCCTGTATCCCAGGAGGCTGGCCAGCTCCTCAGCTGCCCCACAGCTCCAGGCTGGCCATCAGCTCCCCTGTACTCAG CTACCAGTGTGCAGCCCTCGGGAGCACCTGTAGAGAGCAGGTCTACCTCCCTCCTGCAGCCCCTGCCAGCCAGTGCGGGAGCCAGCTGTTCCCGATGCCT CTGGGCCCCCCAGCCTCTCCAGGTGCCCTGTCTCCCTGTCTCCCAGCCCTGGTCCCAACCCCAGCCCCAGCCCCAGCCCCATGCTGGCAGAGAGCTGCTC CTACGAGTCCCCAAGGTGCTTGGCTCTTCACAGGGCCAGGCAGCTCCAGATTGAAGGGCCACAGATACAACTTACTCCTCTGCTGGGTGCTGCACACCCG GCCAGGGCTGACCAGGACGCAGGAGGGGCAGAACCCCAGGACTTCCCCAAAAGAGCTAGAAGGAGGCCCGGGCCTGGCCTTTGAAGTCCAGCAAGCAGCT GTCACGCTCAGCTCCATGGAGCTGACAGAGCCACCCCTCCCACTGGCCAGGTCTTTGGGAGAGAATCTGGGCTGGGGGCTGCCCTCTCCTACCCCTGCCT CCTGCCATGTTCCTGACCAGGCAGAGTCTACAGTGGGCTTGCCCAGCTGAGGCTGGCCGCCCACGCCAGGAGGCAGAGGCGAAGTTGGCATGGGGAGGGA CCACAAAGGAGCTGGGGCAGCAGGAGTCCTGGCCCGGCTTCCTGGCCCTCCACAGGCCCTTGCCTCTCTGCAGAGCCGCGGGTTAGCTGTTGGTGTGCTC CACCCCCAACCCCGGCCTCCTTTCTCACGAGCATGTGGCCGCTCCTGCTTTCCAAAATGACTGTGTTTGTCCCCTCCCCCAGCCCCCCACGCTCCCGCAC ACAGATCCTCAGGAACATATGAAGCAGAGGAGGGGCTTGGGCTGCACCACTCACAGAGCTCCCTCCCCCAGGCACTTAGTTGGGGCCCAGCACTGACCTT TCCCCTGAGCCCAGGATGTGGCCAGAGCCCCCTCTGGGACCCCTCTCGCCCCTTCTCTGCCTCCTCAGCTTGAGCTGCCTGCCCGAAGTTCGGCTGTTCC GGGGCCAGTGTGTCACCTGCCAACTTCCACATCACCCTCCTCCCTCGCTCCCTCCTCTCCTTCCCCAAGGACCTCCCCCCATTTCTGGCAGCCAAGCCAT TAATCTGGAGACAGAAATGGGTTTGCTATCGATTCTCTGGCCACTTTTTCTTTCATTACAATTTGTACCGTGATTCTTCTCACCCTTCTCTGCGTCCGTG CATTTAAAGAGTTGTCTCTTTAAATGTTGAAGCTTCCGGAAGCCTGATGCTATTCTGTGTCTCCTTTCAAAGGAAGAAGGGGGGGCCCAGCTATGGGTGA GGACTCAAGTTATTAGTTTGGAAATAGAGCAACTATGTGTACAGCCCACCTTAGAGGTCATGTTACCCCCTTCCTGTTAAATTTTACAATTAATTTTGGT TCAGGAAATGTAAATAAATTTGTTAATTACAAATAGCAAAGAGATGGGGCTGGAATGCCATATGTCGGTCCTTGATCTGCCCTCCAGTGACCCAAGGCAG GGCTGCGGGCCAGCAGCGATGGACAGAATCACTAGATGGTGTGATGGGACCTCCTGCAAGTCACACATGTGTGAGTGCCACAACCCTCCTGCCGGCCTTG CCCAAGGGCTCCAGGGAGCAGGGCTGCCTCTGTCCCTGCCCAGCCTCCTTTACCCAGTCTCATCTGGTCACTTCCTTGGCTCTGCTGCCTGGGGAGTGGT CGCCTGCAGAGGACATGCCCCTCCATCCCTCCCAAGAAAACTCCTTTGCCCCGGCGGACATTCCTGTTAAGGGCCCACTCCTCTTCCCTGAAGCCAGACC GTGGCTTATGTCTGCACGCTGCTGGGTTCGTCCCTTTCCTTTTGTGACATGGGGTTGCCTCATTCCCCATGACACCACCCCTCTGCATGAGGAAGTTTCC CCTTGCCCCTGTCTCAGACTTGGATACCTTTACCTGCTGCTCACACAAAGCCTGTGGTGCCGGGTTCGGGTGCCCTCATTGTCAACTGCAGG

DRF1.F1 (forward primer)
DRF1.R1 (reverse primer)
Alternative acceptor

Frame 0

LDFLPCLSLLEEC*HV*PLLVFHLRVQLSSSHS MASLTATALQWCLKVLSKP*GTFCSKMSCIQCCK RA*HPLRCCSRDRGPSP*PPLSGDAEGMCSQKV AGKAGVIQWAGPASLVLQPRKGLC*VSEDWKPL AQTLCLLHRNDDARATAVSCVFMCEKTTAKEARG NMSSSRVKNTESGQTEGPVPQNRR*KQEVSSFP SSV*ILS*NFFSWTQRCKSL*GPDDPGQHAPYQ RIQGWRAKPTISCPHHAQEEERLLRVLSGGLRGA PCASSECPAPELCPGSPSICRSGQDHCSAQPQL CRHPFPGWPPQVVRFPSF*L*PSLS*DSAPPSA LPSQGSISQDKERRQLPGIRYPRAGWDSGQHAGT S*TCRDW*GTWTYSKLPGPGGVS*CVCGPPRDT SVQEPCLPMPPA*LWFYGTLLWPRPGTFWPQAE GSVPQWQC*EESGGLLATGFIFCPNSPQPLRHQD N*WQASSLSSPYRP*VQAPRLPPALVPLTDLPL SPRPLPLAAHRQTSRVLGHTALLAGERVAPRA* GF*VYSHWPCIPGGWPAPQLPHSSRLAISSPVLS YQCAALGSTCREQVYLPPAAPASQCGSQLFPMP LGPPASPGALSPCLPALVPTPAPAPAPCWQRAA PTSPQGAWLFTGPGSSRLKGHRYNLLLCWVLHTR PGLTRTQEGQNPRTSPKELEGGPGLAFEVQQAA VTLSSMELTEPPLPLARSLGENLGWGLPSPTPA SCHVPDQAESTVGLPS*GWPPTPGGRGEVGMGRD HKGAGAAGVLARLPGPPQALASLQSRGLAVGVL HPQPRPPFSRACGRSCFPK*LCLSPPPAPHAPA HRSSGTYEAEEGLGLHHSQSSLPQALSWGPALTF PLSPGCGQSPLWDPSRPFSASSA*AACPKFGCS GASVSPANFHITLLPRSLLSFPKDLPPFLAAKP LIWRQKWVCYRFSGHFFFHYNLYRDSSHPSLRPC I*RVVSLNVEASGSLMLFCVSFQRKKGGPSYG* GLKLLVWK*SNYVYSPP*RSCYPLPVKFYN*FW FRKCK*IC*LQIAKRWGWNAICRSLICPPVTQGR AAGQQRWTESLDGVMGPPASHTCVSATTLLPAL PKGSREQGCLCPCPASFTQSHLVTSLALLPGEW SPAEDMPLHPSQENSFAPADIPVKGPLLFPEARP WLMSARCWVRPFPFVTWGCLIPHDTTPLHEEVS PCPCLRLGYLYLLLTQSLWCRVRVPSLSTA

Frame 1

LISFPAFLC*RSASMFSPCWSFT*GYS*VVHTA WPPSQPQLFSGALKSLASHEAPSVQR*VVFNAA KEHDTRSGAVPGIEDPVLNLRCQEMQRGCVARRW QARLE*FSGLAQRV*SCSPGRGCAR*VKTGSLW HKPSVFSTEMMMHVQQLSLASLCVKKQQPKKPE GTCPAAESRTRKVARLKAPFLKIEDESRKFRPFH HQFKSFPEISFLGPKDASPFEAPTTLGSMHHTR ESKDGEPSPRSAAHTMPRRKKGYCECCQEAFEE LHVHLQSAQHRSFALEAHLYAEVDRIIAQLSHSF ADIPFQAGLPRWSGSPASDCDPLCPETLHPHQP SHPRAASPRIRKEDSCQASGIPEQDGTVDSTQA PAERAGTGEVPGPIASCQDLGVSVDVFVDPPGIP VSRSPACQCLLPSSGFMELSSGPDLALFGHKRK VQFPSGSAKKRVGASWPQASFFVPIAPNPCGTR TTSGKRLPSLPLTGHESRLLASLQPLCHSQTCLS LPDPFPWQPTDRPAEFWATQPSWLGKGWPPGPE DSECTATGPVSQEAGQLLSCPTAPGWPSAPLYS ATSVQPSGAPVESRSTSLLQPLPASAGASCSRCL WAPQPLQVPCLPVSQPWSQPQPQPQPHAGRELL LRVPKVLGSSQGQAAPD*RATDTTYSSAGCCTP GQG*PGRRRGRTPGLPQKS*KEARAWPLKSSKQL SRSAPWS*QSHPSHWPGLWERIWAGGCPLLPLP PAMFLTRQSLQWACPAEAGRPRQEAEAKLAWGG TTKELGQQESWPGFLALHRPLPLCRAAG*LLVCS TPNPGLLSHEHVAAPAFQNDCVCPLPQPPTLPH TDPQEHMKQRRGLGCTTHRAPSPRHLVGAQH*P FP*AQDVARAPSGTPLAPSLPPQLELPARSSAVP GPVCHLPTSTSPSSLAPSSPSPRTSPHFWQPSH *SGDRNGFAIDSLATFSFITICTVILLTLLCVR AFKELSL*MLKLPEA*CYSVSPFKGRRGGPAMGE DSSY*FGNRATMCTAHLRGHVTPFLLNFTINFG SGNVNKFVNYK*QRDGAGMPYVGP*SALQ*PKA GLRASSDGQNH*MV*WDLLQVTHV*VPQPSCRPC PRAPGSRAASVPAQPPLPSLIWSLPWLCCLGSG RLQRTCPSIPPKKTPLPRRTFLLRAHSSSLKPD RGLCLHAAGFVPFLL*HGVASFPMTPPLCMRKFP LAPVSDLDTFTCCSHKACGAGFGCPHCQLQ

Frame 2

*FPSLPFSVRGVLACLALAGLSPEGTAE*FTQH GLPHSHSSSVVP*SP*QAMRHLLFKDELYSMLQ KSMTPAQVLFQG*RTQSLTSAVRRCRGDV*PEG GRQGWSDSVGWPSEFSPAAQEGAVLGK*RLEAFG TNPLSSPQK**CTCNSCLLRLYV*KNNSQRSQR EHVQQQSQEHGKWPD*RPRSSKSKMKAGSFVLS IISLNPFLKFLFLDPKMQVPLRPRRPWAACTIPE NPRMESQAHDQLPTPCPGGRKATASAVRRPSRS SMCIFRVPSTGALPWKPIYMQKWTGSLLSSATA LQTSLSRLASPGGQVPQLLIVTLSVLRLCTPISP PIPGQHLPG*GKKTAARHQVSQSRMGQWTARRH QLNVQGLVRYLDL*QAARTWGCQLMCLWTPQGY QCPGALLANASCLALVLWNSPLAQTWHFLATSGR FSSPVAVLRREWGPPGHRLHFLSQ*PPTPAAPG QLVASVFPLFPLQAMSPGSSPPSSPCATHRPAS LSQTPSPGSPQTDQQSSGPHSPPGWGKGGPQGLR ILSVQPLALYPRRLASSSAAPQLQAGHQLPCTQ LPVCSPREHL*RAGLPPSCSPCQPVREPAVPDA SGPPSLSRCPVSLSPSPGPNPSPSPSPMLAESCS YESPRCLALHRARQLQIEGPQIQLTPLLGAAHP ARADQDAGGAEPQDFPKRARRRPGPGL*SPASS CHAQLHGADRATPPTGQVFGRESGLGAALSYPCL LPCS*PGRVYSGLAQLRLAAHARRQRRSWHGEG PQRSWGSRSPGPASWPSTGPCLSAEPRVSCWCA PPPTPASFLTSMWPLLLSKMTVFVPSPSPPRSRT QILRNI*SRGGAWAAPLTELPPPGT*LGPSTDL SPEPRMWPEPPLGPLSPLLCLLSLSCLPEVRLF RGQCVTCQLPHHPPPSLPPLLPQGPPPISGSQAI NLETEMGLLSILWPLFLSLQFVP*FFSPFSASV HLKSCLFKC*SFRKPDAILCLLSKEEGGAQLWV RTQVISLEIEQLCVQPTLEVMLPPSC*ILQLILV QEM*INLLITNSKEMGLECHMSVLDLPSSDPRQ GCGPAAMDRITRWCDGTSCKSHMCECHNPPAGL AQGLQGAGLPLSLPSLLYPVSSGHFLGSAAWGVV ACRGHAPPSLPRKLLCPGGHSC*GPTPLP*SQT VAYVCTLLGSSLSFCDMGLPHSP*HHPSA*GSF PLPLSQTWIPLPAAHTKPVVPGSGALIVNCR

Sequence DRF1.eAug05

Frame 0

Frame 1

Frame 2

Sequence DRF1.aAug05

Frame 0

Frame 1

Frame 2

Sequence DRF1.bAug05

Frame 0

Frame 1

Frame 2

Sequence DRF1.cAug05

Frame 0

Frame 1

Frame 2

Sequence DRF1.dAug05

Frame 0

Frame 1

Frame 2