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Sequences

Sequence NM_138615

GCCGCGGCTCATGCGCGGTGCACAGAGGCTTGTTTCACATCTGTAACAACAGGAGGAGGCCCAGCCTCGTGATGAGGAATAGCAAGGAGAGAATTCAGCT CCAGTTCAAAAGCCTACAAAATCTGAGACTGTCATTGCTTTTATAAGGATTCCAGCTTTCCCTCCTGGCCAGAAATGTTCAGCCTGGACTCATTCAGAAA AGATCGGGCCCAGCACAGGCAGCGTCAGTGCAAACTTCCCCCACCCCGCCTTCCACCCATGTGTGTCAACCCTACCCCAGGAGGGACCATCTCTCGAGCT TCTAGGGACCTATTAAAAGAGTTCCCACAGCCCAAAAATCTTCTCAACAGTGTGATTGGAAGAGCCCTCGGCATCTCACATGCAAAAGACAAACTAGTCT ACGTGCACACAAATGGACCGAAGAAAAAGAAAGTCACACTGCACATAAAATGGCCCAAGAGCGTGGAGGTAGAAGGCTATGGCAGCAAGAAGATCGATGC TGAGCGGCAGGCTGCAGCTGCAGCCTGCCAGCTGTTCAAGGGTTGGGGTCTGCTAGGTCCCCGGAATGAGTTGTTTGACGCAGCCAAATACCGAGTGCTA GCTGATCGCTTTGGCTCCCCTGCCGACAGCTGGTGGCGTCCGGAACCCACCATGCCCCCTACTTCCTGGCGGCAGCTGAATCCAGAGAGTATTCGACCAG GGGGACCTGGGGGCCTATCCCGCTCTTTAGGCCGGGAAGAAGAGGAGGACGAGGAGGAAGAGCTAGAAGAAGGGACCATAGATGTTACCGACTTCTTGTC CATGACCCAGCAGGATTCCCACGCTCCACTCAGGGACTCAAGGGGGAGTTCCTTTGAGATGACAGATGACGACAGTGCCATTAGGGCTCTGACCCAGTTT CCACTTCCCAAGAACCTTCTGGCCAAGGTGATTCAGATTGCAACGTCATCCTCCACAGCTAAGAACCTCATGCAGTTCCATACTGTGGGCACCAAGACCA AGCTGTCTACACTCACCCTGCTCTGGCCCTGCCCCATGACCTTTGTTGCCAAAGGGCGCCGCAAAGCAGAGGCTGAGAATAAGGCGGCAGCCTTGGCCTG CAAGAAACTGAAGAGCCTGGGCCTGGTGGACAGGAACAACGAACCGCTTACACACGCCATGTATAACCTGGCCTCTTTGCGTGAGCTGGGTGAGACCCAG CGCCGACCATGCACCATCCAGGTGCCCGAGCCCATCCTCCGCAAGATAGAGACCTTCCTGAACCATTACCCTGTGGAGAGTTCATGGATCGCCCCAGAAC TCCGGCTGCAGAGTGATGACATCTTGCCCTTGGGCAAGGACTCAGGGCCTCTGAGTGACCCTATCACAGGCAAGCCCTATGTGCCCCTGTTGGAAGCAGA GGAGGTACGTCTCAGCCAGAGTCTGCTAGAACTGTGGCGGCGGCGAGGGCCGGTCTGGCAGGAGGCCCCCCAGCTACCTGTGGACCCACATCGGGACACC ATCCTCAACGCCATTGAGCAGCACCCGGTGGTGGTCATCTCTGGGGACACGGGCTGTGGGAAGACCACGCGCATCCCCCAGCTGTTGCTGGAGCGCTATG TGACCGAGGGCCGAGGTGCCCGCTGCAATGTTATCATCACCCAACCTCGCCGCATCTCTGCTGTGTCTGTGGCACAGCGGGTCAGCCACGAACTGGGCCC CTCCCTGCGCCGGAATGTGGGCTTCCAGGTGCGGTTGGAAAGTAAGCCCCCATCCCGAGGCGGGGCCCTGCTCTTCTGCACTGTGGGTATCCTGCTGCGT AAGCTGCAGAGCAACCCCAGCCTGGAGGGCGTGAGCCACGTCATCGTGGATGAGGTGCATGAGCGGGACGTGAACACAGACTTTCTGCTGATCCTGCTCA AGGGCCTGCAGCGGCTCAACCCGGCCCTGCGGCTGGTGCTCATGAGTGCCACAGGGGACAATGAGCGCTTCTCCCGATACTTTGGTGGCTGCCCCGTCAT CAAGGTGCCTGGCTTCATGTACCCAGTCAAGGAGCACTACCTAGAGGACATCCTGGCCAAGTTGGGCAAGCACCAGTACCTGCACCGGCACCGGCACCAT GAGTCTGAGGATGAATGCGCACTCGATTTGGACCTTGTGACTGATCTGGTTCTGCACATCGATGCTCGCGGGGAACCAGGTGGGATCCTGTGCTTCCTGC CTGGGTGGCAGGAGATCAAAGGAGTGCAGCAGCGCCTCCAGGAGGCCCTGGGCATGCACGAGAGCAAGTACCTCATCCTGCCAGTGCACTCCAACATCCC CATGATGGATCAGAAGGCCATATTCCAGCAGCCTCCAGTTGGGGTGCGCAAGATTGTCTTGGCCACCAACATTGCTGAGACTTCCATCACAATCAATGAC ATCGTGCATGTGGTGGACAGTGGGCTGCACAAGGAAGAACGCTATGACCTGAAGACCAAGGTGTCCTGCCTGGAGACAGTGTGGGTATCAAGAGCCAATG TGATCCAGCGCCGGGGCCGGGCGGGCCGCTGCCAGTCCGGCTTTGCCTACCACTTGTTCCCTCGAAGCCGGCTGGAGAAAATGGTCCCTTTCCAAGTGCC AGAGATCCTGCGCACACCTCTTGAGAACCTGGTGCTGCAAGCGAAAATCCACATGCCTGAGAAGACGGCGGTGGAGTTCCTGTCCAAGGCTGTGGACAGT CCAAACATCAAGGCAGTGGACGAGGCTGTGATCTTGCTCCAGGAGATCGGGGTGCTGGACCAGCGGGAGTACCTGACTACCCTGGGGCAGCGCCTGGCTC ACATCTCCACCGACCCCCGGTTGGCCAAGGCCATTGTGTTGGCTGCCATCTTCCGTTGCCTGCACCCACTACTGGTGGTCGTTTCCTGCCTCACCCGGGA CCCCTTCAGCAGCAGCCTACAGAACCGGGCAGAGGTGGACAAGGTGAAAGCACTGTTGAGCCATGACAGCGGCAGTGACCACCTGGCCTTTGTGCGGGCT GTCGCCGGCTGGGAGGAGGTGCTGCGTTGGCAGGACCGCAGCTCCCGGGAGAATTACCTGGAGGAAAACCTGCTGTACGCACCCAGCCTGCGCTTCATCC ACGGACTCATCAAGCAGTTCTCAGAGAACATTTATGAGGCCTTCCTGGTGGGGAAGCCCTCGGACTGCACCCTGGCCTCCGCCCAGTGCAACGAGTACAG TGAGGAGGAGGAGCTGGTGAAGGGCGTGCTGATGGCCGGCCTCTACCCCAACCTCATCCAGGTGAGGCAGGGCAAGGTCACCCGGCAGGGGAAGTTCAAG CCCAACAGCGTCACATATAGGACCAAATCAGGCAACATCCTGCTGCACAAGTCGACCATTAACAGGGAGGCCACACGGTTACGGAGCCGATGGCTGACGT ATTTCATGGCAGTCAAGTCCAATGGCAGCGTCTTCGTCCGGGACTCCTCTCAGGTGCACCCGCTAGCTGTGCTGCTGCTGACCGACGGGGACGTGCACAT CCGTGATGACGGGCGCCGGGCCACCATCTCACTGAGCGACAGTGACCTGCTGCGGCTGGAGGGTGACTCGCGTACCGTGCGGCTGCTGAAGGAGCTGCGG CGGGCCCTGGGCCGCATGGTGGAGCGGAGCCTGCGCAGCGAGCTGGCTGCACTTCCCCCCAGCGTACAGGAGGAGCACGGGCAGCTGCTTGCGCTACTGG CAGAGCTGCTGCGAGGACCCTGTGGCAGCTTTGATGTGCGCAAGACAGCTGACGACTGAGCCCTGCTTCTGCTGGGGCTGTGTACAGAGTGCAAATGTTT ATTTAAAATAAAGTTCTATTTATCCCTTGTGA

refseq_DHX30.F1 (forward primer)
refseq_DHX30.R1 (reverse primer)
Single exon skipping

Frame 0

AAAHARCTEACFTSVTTGGGPAS**GIARREFS SSSKAYKI*DCHCFYKDSSFPSWPEMFSLDSFRK DRAQHRQRQCKLPPPRLPPMCVNPTPGGTISRA SRDLLKEFPQPKNLLNSVIGRALGISHAKDKLV YVHTNGPKKKKVTLHIKWPKSVEVEGYGSKKIDA ERQAAAAACQLFKGWGLLGPRNELFDAAKYRVL ADRFGSPADSWWRPEPTMPPTSWRQLNPESIRP GGPGGLSRSLGREEEEDEEEELEEGTIDVTDFLS MTQQDSHAPLRDSRGSSFEMTDDDSAIRALTQF PLPKNLLAKVIQIATSSSTAKNLMQFHTVGTKT KLSTLTLLWPCPMTFVAKGRRKAEAENKAAALAC KKLKSLGLVDRNNEPLTHAMYNLASLRELGETQ RRPCTIQVPEPILRKIETFLNHYPVESSWIAPE LRLQSDDILPLGKDSGPLSDPITGKPYVPLLEAE EVRLSQSLLELWRRRGPVWQEAPQLPVDPHRDT ILNAIEQHPVVVISGDTGCGKTTRIPQLLLERY VTEGRGARCNVIITQPRRISAVSVAQRVSHELGP SLRRNVGFQVRLESKPPSRGGALLFCTVGILLR KLQSNPSLEGVSHVIVDEVHERDVNTDFLLILL KGLQRLNPALRLVLMSATGDNERFSRYFGGCPVI KVPGFMYPVKEHYLEDILAKLGKHQYLHRHRHH ESEDECALDLDLVTDLVLHIDARGEPGGILCFL PGWQEIKGVQQRLQEALGMHESKYLILPVHSNIP MMDQKAIFQQPPVGVRKIVLATNIAETSITIND IVHVVDSGLHKEERYDLKTKVSCLETVWVSRAN VIQRRGRAGRCQSGFAYHLFPRSRLEKMVPFQVP EILRTPLENLVLQAKIHMPEKTAVEFLSKAVDS PNIKAVDEAVILLQEIGVLDQREYLTTLGQRLA HISTDPRLAKAIVLAAIFRCLHPLLVVVSCLTRD PFSSSLQNRAEVDKVKALLSHDSGSDHLAFVRA VAGWEEVLRWQDRSSRENYLEENLLYAPSLRFI HGLIKQFSENIYEAFLVGKPSDCTLASAQCNEYS EEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFK PNSVTYRTKSGNILLHKSTINREATRLRSRWLT YFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHI RDDGRRATISLSDSDLLRLEGDSRTVRLLKELR RALGRMVERSLRSELAALPPSVQEEHGQLLALL AELLRGPCGSFDVRKTADD*ALLLLGLCTECKCL FKIKFYLSLV

Frame 1

PRLMRGAQRLVSHL*QQEEAQPRDEE*QGENSA PVQKPTKSETVIAFIRIPAFPPGQKCSAWTHSE KIGPSTGSVSANFPHPAFHPCVSTLPQEGPSLEL LGTY*KSSHSPKIFSTV*LEEPSASHMQKTN*S TCTQMDRRKRKSHCT*NGPRAWR*KAMAARRSM LSGRLQLQPASCSRVGVC*VPGMSCLTQPNTEC* LIALAPLPTAGGVRNPPCPLLPGGS*IQRVFDQ GDLGAYPAL*AGKKRRTRRKS*KKGP*MLPTSC P*PSRIPTLHSGTQGGVPLR*QMTTVPLGL*PSF HFPRTFWPR*FRLQRHPPQLRTSCSSILWAPRP SCLHSPCSGPAP*PLLPKGAAKQRLRIRRQPWP ARN*RAWAWWTGTTNRLHTPCITWPLCVSWVRPS ADHAPSRCPSPSSAR*RPS*TITLWRVHGSPQN SGCRVMTSCPWARTQGL*VTLSQASPMCPCWKQ RRYVSARVC*NCGGGEGRSGRRPPSYLWTHIGTP SSTPLSSTRWWSSLGTRAVGRPRASPSCCWSAM *PRAEVPAAMLSSPNLAASLLCLWHSGSATNWA PPCAGMWASRCGWKVSPHPEAGPCSSALWVSCCV SCRATPAWRA*ATSSWMRCMSGT*TQTFC*SCS RACSGSTRPCGWCS*VPQGTMSASPDTLVAAPS SRCLASCTQSRSTT*RTSWPSWASTSTCTGTGTM SLRMNAHSIWTL*LIWFCTSMLAGNQVGSCASC LGGRRSKECSSASRRPWACTRASTSSCQCTPTS P*WIRRPYSSSLQLGCARLSWPPTLLRLPSQSMT SCMWWTVGCTRKNAMT*RPRCPAWRQCGYQEPM *SSAGAGRAAASPALPTTCSLEAGWRKWSLSKC QRSCAHLLRTWCCKRKSTCLRRRRWSSCPRLWTV QTSRQWTRL*SCSRRSGCWTSGST*LPWGSAWL TSPPTPGWPRPLCWLPSSVACTHYWWSFPASPG TPSAAAYRTGQRWTR*KHC*AMTAAVTTWPLCGL SPAGRRCCVGRTAAPGRITWRKTCCTHPACASS TDSSSSSQRTFMRPSWWGSPRTAPWPPPSATST VRRRSW*RAC*WPASTPTSSR*GRARSPGRGSSS PTASHIGPNQATSCCTSRPLTGRPHGYGADG*R ISWQSSPMAASSSGTPLRCTR*LCCC*PTGTCT SVMTGAGPPSH*ATVTCCGWRVTRVPCGC*RSCG GPWAAWWSGACAASWLHFPPAYRRSTGSCLRYW QSCCEDPVAALMCARQLTTEPCFCWGCVQSANV YLK*SSIYPL*

Frame 2

RGSCAVHRGLFHICNNRRRPSLVMRNSKERIQL QFKSLQNLRLSLLL*GFQLSLLARNVQPGLIQK RSGPAQAASVQTSPTPPSTHVCQPYPRRDHLSS F*GPIKRVPTAQKSSQQCDWKSPRHLTCKRQTSL RAHKWTEEKESHTAHKMAQERGGRRLWQQEDRC *AAGCSCSLPAVQGLGSARSPE*VV*RSQIPSA S*SLWLPCRQLVASGTHHAPYFLAAAESREYSTR GTWGPIPLFRPGRRGGRGGRARRRDHRCYRLLV HDPAGFPRSTQGLKGEFL*DDR*RQCH*GSDPV STSQEPSGQGDSDCNVILHS*EPHAVPYCGHQDQ AVYTHPALALPHDLCCQRAPQSRG*E*GGSLGL QETEEPGPGGQEQRTAYTRHV*PGLFA*AG*DP APTMHHPGARAHPPQDRDLPEPLPCGEFMDRPRT PAAE**HLALGQGLRASE*PYHRQALCAPVGSR GGTSQPESARTVAAARAGLAGGPPATCGPTSGH HPQRH*AAPGGGHLWGHGLWEDHAHPPAVAGALC DRGPRCPLQCYHHPTSPHLCCVCGTAGQPRTGP LPAPECGLPGAVGK*APIPRRGPALLHCGYPAA *AAEQPQPGGREPRHRG*GA*AGREHRLSADPAQ GPAAAQPGPAAGAHECHRGQ*ALLPILWWLPRH QGAWLHVPSQGALPRGHPGQVGQAPVPAPAPAP *V*G*MRTRFGPCD*SGSAHRCSRGTRWDPVLPA WVAGDQRSAAAPPGGPGHAREQVPHPASALQHP HDGSEGHIPAASSWGAQDCLGHQHC*DFHHNQ* HRACGGQWAAQGRTL*PEDQGVLPGDSVGIKSQC DPAPGPGGPLPVRLCLPLVPSKPAGENGPFPSA RDPAHTS*EPGAASENPHA*EDGGGVPVQGCGQ SKHQGSGRGCDLAPGDRGAGPAGVPDYPGAAPGS HLHRPPVGQGHCVGCHLPLPAPTTGGRFLPHPG PLQQQPTEPGRGGQGESTVEP*QRQ*PPGLCAG CRRLGGGAALAGPQLPGELPGGKPAVRTQPALHP RTHQAVLREHL*GLPGGEALGLHPGLRPVQRVQ *GGGAGEGRADGRPLPQPHPGEAGQGHPAGEVQ AQQRHI*DQIRQHPAAQVDH*QGGHTVTEPMADV FHGSQVQWQRLRPGLLSGAPASCAAADRRGRAH P**RAPGHHLTERQ*PAAAGG*LAYRAAAEGAA AGPGPHGGAEPAQRAGCTSPQRTGGARAAACATG RAAARTLWQL*CAQDS*RLSPASAGAVYRVQMF I*NKVLFIPC

Sequence NM_014966

GCCGCGGCTCATGCGCGGTGCACAGAGGCTTGTTTCACATCTGTAACAACAGGAGGAGGCCCAGCCTCGTGATGAGGAATAGCAAGGAGAGAATTCAGCT CCAGTTCAAAAGCCTACAAAATCTGAGACTGTCATTGCTTTTATAAGGATTCCAGCTTTCCCTCCTGGCCAGAAATGTTCAGCCTGGACTCATTCAGAAA AGATCGGGCCCAGCACAGGCAGCGTCAGTGCAAACTTCCCCCACCCCGCCTTCCACCCATGTGTGTCAACCCTACCCCAGGAGGGACCATCTCTCGAGGC TGAACGTTAACATTTCCAACATGGCAGCTTCTAGGGACCTATTAAAAGAGTTCCCACAGCCCAAAAATCTTCTCAACAGTGTGATTGGAAGAGCCCTCGG CATCTCACATGCAAAAGACAAACTAGTCTACGTGCACACAAATGGACCGAAGAAAAAGAAAGTCACACTGCACATAAAATGGCCCAAGAGCGTGGAGGTA GAAGGCTATGGCAGCAAGAAGATCGATGCTGAGCGGCAGGCTGCAGCTGCAGCCTGCCAGCTGTTCAAGGGTTGGGGTCTGCTAGGTCCCCGGAATGAGT TGTTTGACGCAGCCAAATACCGAGTGCTAGCTGATCGCTTTGGCTCCCCTGCCGACAGCTGGTGGCGTCCGGAACCCACCATGCCCCCTACTTCCTGGCG GCAGCTGAATCCAGAGAGTATTCGACCAGGGGGACCTGGGGGCCTATCCCGCTCTTTAGGCCGGGAAGAAGAGGAGGACGAGGAGGAAGAGCTAGAAGAA GGGACCATAGATGTTACCGACTTCTTGTCCATGACCCAGCAGGATTCCCACGCTCCACTCAGGGACTCAAGGGGGAGTTCCTTTGAGATGACAGATGACG ACAGTGCCATTAGGGCTCTGACCCAGTTTCCACTTCCCAAGAACCTTCTGGCCAAGGTGATTCAGATTGCAACGTCATCCTCCACAGCTAAGAACCTCAT GCAGTTCCATACTGTGGGCACCAAGACCAAGCTGTCTACACTCACCCTGCTCTGGCCCTGCCCCATGACCTTTGTTGCCAAAGGGCGCCGCAAAGCAGAG GCTGAGAATAAGGCGGCAGCCTTGGCCTGCAAGAAACTGAAGAGCCTGGGCCTGGTGGACAGGAACAACGAACCGCTTACACACGCCATGTATAACCTGG CCTCTTTGCGTGAGCTGGGTGAGACCCAGCGCCGACCATGCACCATCCAGGTGCCCGAGCCCATCCTCCGCAAGATAGAGACCTTCCTGAACCATTACCC TGTGGAGAGTTCATGGATCGCCCCAGAACTCCGGCTGCAGAGTGATGACATCTTGCCCTTGGGCAAGGACTCAGGGCCTCTGAGTGACCCTATCACAGGC AAGCCCTATGTGCCCCTGTTGGAAGCAGAGGAGGTACGTCTCAGCCAGAGTCTGCTAGAACTGTGGCGGCGGCGAGGGCCGGTCTGGCAGGAGGCCCCCC AGCTACCTGTGGACCCACATCGGGACACCATCCTCAACGCCATTGAGCAGCACCCGGTGGTGGTCATCTCTGGGGACACGGGCTGTGGGAAGACCACGCG CATCCCCCAGCTGTTGCTGGAGCGCTATGTGACCGAGGGCCGAGGTGCCCGCTGCAATGTTATCATCACCCAACCTCGCCGCATCTCTGCTGTGTCTGTG GCACAGCGGGTCAGCCACGAACTGGGCCCCTCCCTGCGCCGGAATGTGGGCTTCCAGGTGCGGTTGGAAAGTAAGCCCCCATCCCGAGGCGGGGCCCTGC TCTTCTGCACTGTGGGTATCCTGCTGCGTAAGCTGCAGAGCAACCCCAGCCTGGAGGGCGTGAGCCACGTCATCGTGGATGAGGTGCATGAGCGGGACGT GAACACAGACTTTCTGCTGATCCTGCTCAAGGGCCTGCAGCGGCTCAACCCGGCCCTGCGGCTGGTGCTCATGAGTGCCACAGGGGACAATGAGCGCTTC TCCCGATACTTTGGTGGCTGCCCCGTCATCAAGGTGCCTGGCTTCATGTACCCAGTCAAGGAGCACTACCTAGAGGACATCCTGGCCAAGTTGGGCAAGC ACCAGTACCTGCACCGGCACCGGCACCATGAGTCTGAGGATGAATGCGCACTCGATTTGGACCTTGTGACTGATCTGGTTCTGCACATCGATGCTCGCGG GGAACCAGGTGGGATCCTGTGCTTCCTGCCTGGGTGGCAGGAGATCAAAGGAGTGCAGCAGCGCCTCCAGGAGGCCCTGGGCATGCACGAGAGCAAGTAC CTCATCCTGCCAGTGCACTCCAACATCCCCATGATGGATCAGAAGGCCATATTCCAGCAGCCTCCAGTTGGGGTGCGCAAGATTGTCTTGGCCACCAACA TTGCTGAGACTTCCATCACAATCAATGACATCGTGCATGTGGTGGACAGTGGGCTGCACAAGGAAGAACGCTATGACCTGAAGACCAAGGTGTCCTGCCT GGAGACAGTGTGGGTATCAAGAGCCAATGTGATCCAGCGCCGGGGCCGGGCGGGCCGCTGCCAGTCCGGCTTTGCCTACCACTTGTTCCCTCGAAGCCGG CTGGAGAAAATGGTCCCTTTCCAAGTGCCAGAGATCCTGCGCACACCTCTTGAGAACCTGGTGCTGCAAGCGAAAATCCACATGCCTGAGAAGACGGCGG TGGAGTTCCTGTCCAAGGCTGTGGACAGTCCAAACATCAAGGCAGTGGACGAGGCTGTGATCTTGCTCCAGGAGATCGGGGTGCTGGACCAGCGGGAGTA CCTGACTACCCTGGGGCAGCGCCTGGCTCACATCTCCACCGACCCCCGGTTGGCCAAGGCCATTGTGTTGGCTGCCATCTTCCGTTGCCTGCACCCACTA CTGGTGGTCGTTTCCTGCCTCACCCGGGACCCCTTCAGCAGCAGCCTACAGAACCGGGCAGAGGTGGACAAGGTGAAAGCACTGTTGAGCCATGACAGCG GCAGTGACCACCTGGCCTTTGTGCGGGCTGTCGCCGGCTGGGAGGAGGTGCTGCGTTGGCAGGACCGCAGCTCCCGGGAGAATTACCTGGAGGAAAACCT GCTGTACGCACCCAGCCTGCGCTTCATCCACGGACTCATCAAGCAGTTCTCAGAGAACATTTATGAGGCCTTCCTGGTGGGGAAGCCCTCGGACTGCACC CTGGCCTCCGCCCAGTGCAACGAGTACAGTGAGGAGGAGGAGCTGGTGAAGGGCGTGCTGATGGCCGGCCTCTACCCCAACCTCATCCAGGTGAGGCAGG GCAAGGTCACCCGGCAGGGGAAGTTCAAGCCCAACAGCGTCACATATAGGACCAAATCAGGCAACATCCTGCTGCACAAGTCGACCATTAACAGGGAGGC CACACGGTTACGGAGCCGATGGCTGACGTATTTCATGGCAGTCAAGTCCAATGGCAGCGTCTTCGTCCGGGACTCCTCTCAGGTGCACCCGCTAGCTGTG CTGCTGCTGACCGACGGGGACGTGCACATCCGTGATGACGGGCGCCGGGCCACCATCTCACTGAGCGACAGTGACCTGCTGCGGCTGGAGGGTGACTCGC GTACCGTGCGGCTGCTGAAGGAGCTGCGGCGGGCCCTGGGCCGCATGGTGGAGCGGAGCCTGCGCAGCGAGCTGGCTGCACTTCCCCCCAGCGTACAGGA GGAGCACGGGCAGCTGCTTGCGCTACTGGCAGAGCTGCTGCGAGGACCCTGTGGCAGCTTTGATGTGCGCAAGACAGCTGACGACTGAGCCCTGCTTCTG CTGGGGCTGTGTACAGAGTGCAAATGTTTATTTAAAATAAAGTTCTATTTATCCCTTGTGA

refseq_DHX30.F1 (forward primer)
refseq_DHX30.R1 (reverse primer)
Single exon skipping

Frame 0

AAAHARCTEACFTSVTTGGGPAS**GIARREFS SSSKAYKI*DCHCFYKDSSFPSWPEMFSLDSFRK DRAQHRQRQCKLPPPRLPPMCVNPTPGGTISRG *TLTFPTWQLLGTY*KSSHSPKIFSTV*LEEPS ASHMQKTN*STCTQMDRRKRKSHCT*NGPRAWR* KAMAARRSMLSGRLQLQPASCSRVGVC*VPGMS CLTQPNTEC*LIALAPLPTAGGVRNPPCPLLPG GS*IQRVFDQGDLGAYPAL*AGKKRRTRRKS*KK GP*MLPTSCP*PSRIPTLHSGTQGGVPLR*QMT TVPLGL*PSFHFPRTFWPR*FRLQRHPPQLRTS CSSILWAPRPSCLHSPCSGPAP*PLLPKGAAKQR LRIRRQPWPARN*RAWAWWTGTTNRLHTPCITW PLCVSWVRPSADHAPSRCPSPSSAR*RPS*TIT LWRVHGSPQNSGCRVMTSCPWARTQGL*VTLSQA SPMCPCWKQRRYVSARVC*NCGGGEGRSGRRPP SYLWTHIGTPSSTPLSSTRWWSSLGTRAVGRPR ASPSCCWSAM*PRAEVPAAMLSSPNLAASLLCLW HSGSATNWAPPCAGMWASRCGWKVSPHPEAGPC SSALWVSCCVSCRATPAWRA*ATSSWMRCMSGT *TQTFC*SCSRACSGSTRPCGWCS*VPQGTMSAS PDTLVAAPSSRCLASCTQSRSTT*RTSWPSWAS TSTCTGTGTMSLRMNAHSIWTL*LIWFCTSMLA GNQVGSCASCLGGRRSKECSSASRRPWACTRAST SSCQCTPTSP*WIRRPYSSSLQLGCARLSWPPT LLRLPSQSMTSCMWWTVGCTRKNAMT*RPRCPA WRQCGYQEPM*SSAGAGRAAASPALPTTCSLEAG WRKWSLSKCQRSCAHLLRTWCCKRKSTCLRRRR WSSCPRLWTVQTSRQWTRL*SCSRRSGCWTSGS T*LPWGSAWLTSPPTPGWPRPLCWLPSSVACTHY WWSFPASPGTPSAAAYRTGQRWTR*KHC*AMTA AVTTWPLCGLSPAGRRCCVGRTAAPGRITWRKT CCTHPACASSTDSSSSSQRTFMRPSWWGSPRTAP WPPPSATSTVRRRSW*RAC*WPASTPTSSR*GR ARSPGRGSSSPTASHIGPNQATSCCTSRPLTGR PHGYGADG*RISWQSSPMAASSSGTPLRCTR*LC CC*PTGTCTSVMTGAGPPSH*ATVTCCGWRVTR VPCGC*RSCGGPWAAWWSGACAASWLHFPPAYR RSTGSCLRYWQSCCEDPVAALMCARQLTTEPCFC WGCVQSANVYLK*SSIYPL*

Frame 1

PRLMRGAQRLVSHL*QQEEAQPRDEE*QGENSA PVQKPTKSETVIAFIRIPAFPPGQKCSAWTHSE KIGPSTGSVSANFPHPAFHPCVSTLPQEGPSLEA ER*HFQHGSF*GPIKRVPTAQKSSQQCDWKSPR HLTCKRQTSLRAHKWTEEKESHTAHKMAQERGG RRLWQQEDRC*AAGCSCSLPAVQGLGSARSPE*V V*RSQIPSAS*SLWLPCRQLVASGTHHAPYFLA AAESREYSTRGTWGPIPLFRPGRRGGRGGRARR RDHRCYRLLVHDPAGFPRSTQGLKGEFL*DDR*R QCH*GSDPVSTSQEPSGQGDSDCNVILHS*EPH AVPYCGHQDQAVYTHPALALPHDLCCQRAPQSR G*E*GGSLGLQETEEPGPGGQEQRTAYTRHV*PG LFA*AG*DPAPTMHHPGARAHPPQDRDLPEPLP CGEFMDRPRTPAAE**HLALGQGLRASE*PYHR QALCAPVGSRGGTSQPESARTVAAARAGLAGGPP ATCGPTSGHHPQRH*AAPGGGHLWGHGLWEDHA HPPAVAGALCDRGPRCPLQCYHHPTSPHLCCVC GTAGQPRTGPLPAPECGLPGAVGK*APIPRRGPA LLHCGYPAA*AAEQPQPGGREPRHRG*GA*AGR EHRLSADPAQGPAAAQPGPAAGAHECHRGQ*AL LPILWWLPRHQGAWLHVPSQGALPRGHPGQVGQA PVPAPAPAP*V*G*MRTRFGPCD*SGSAHRCSR GTRWDPVLPAWVAGDQRSAAAPPGGPGHAREQV PHPASALQHPHDGSEGHIPAASSWGAQDCLGHQH C*DFHHNQ*HRACGGQWAAQGRTL*PEDQGVLP GDSVGIKSQCDPAPGPGGPLPVRLCLPLVPSKP AGENGPFPSARDPAHTS*EPGAASENPHA*EDGG GVPVQGCGQSKHQGSGRGCDLAPGDRGAGPAGV PDYPGAAPGSHLHRPPVGQGHCVGCHLPLPAPT TGGRFLPHPGPLQQQPTEPGRGGQGESTVEP*QR Q*PPGLCAGCRRLGGGAALAGPQLPGELPGGKP AVRTQPALHPRTHQAVLREHL*GLPGGEALGLH PGLRPVQRVQ*GGGAGEGRADGRPLPQPHPGEAG QGHPAGEVQAQQRHI*DQIRQHPAAQVDH*QGG HTVTEPMADVFHGSQVQWQRLRPGLLSGAPASC AAADRRGRAHP**RAPGHHLTERQ*PAAAGG*LA YRAAAEGAAAGPGPHGGAEPAQRAGCTSPQRTG GARAAACATGRAAARTLWQL*CAQDS*RLSPAS AGAVYRVQMFI*NKVLFIPC

Frame 2

RGSCAVHRGLFHICNNRRRPSLVMRNSKERIQL QFKSLQNLRLSLLL*GFQLSLLARNVQPGLIQK RSGPAQAASVQTSPTPPSTHVCQPYPRRDHLSR LNVNISNMAASRDLLKEFPQPKNLLNSVIGRALG ISHAKDKLVYVHTNGPKKKKVTLHIKWPKSVEV EGYGSKKIDAERQAAAAACQLFKGWGLLGPRNE LFDAAKYRVLADRFGSPADSWWRPEPTMPPTSWR QLNPESIRPGGPGGLSRSLGREEEEDEEEELEE GTIDVTDFLSMTQQDSHAPLRDSRGSSFEMTDD DSAIRALTQFPLPKNLLAKVIQIATSSSTAKNLM QFHTVGTKTKLSTLTLLWPCPMTFVAKGRRKAE AENKAAALACKKLKSLGLVDRNNEPLTHAMYNL ASLRELGETQRRPCTIQVPEPILRKIETFLNHYP VESSWIAPELRLQSDDILPLGKDSGPLSDPITG KPYVPLLEAEEVRLSQSLLELWRRRGPVWQEAP QLPVDPHRDTILNAIEQHPVVVISGDTGCGKTTR IPQLLLERYVTEGRGARCNVIITQPRRISAVSV AQRVSHELGPSLRRNVGFQVRLESKPPSRGGAL LFCTVGILLRKLQSNPSLEGVSHVIVDEVHERDV NTDFLLILLKGLQRLNPALRLVLMSATGDNERF SRYFGGCPVIKVPGFMYPVKEHYLEDILAKLGK HQYLHRHRHHESEDECALDLDLVTDLVLHIDARG EPGGILCFLPGWQEIKGVQQRLQEALGMHESKY LILPVHSNIPMMDQKAIFQQPPVGVRKIVLATN IAETSITINDIVHVVDSGLHKEERYDLKTKVSCL ETVWVSRANVIQRRGRAGRCQSGFAYHLFPRSR LEKMVPFQVPEILRTPLENLVLQAKIHMPEKTA VEFLSKAVDSPNIKAVDEAVILLQEIGVLDQREY LTTLGQRLAHISTDPRLAKAIVLAAIFRCLHPL LVVVSCLTRDPFSSSLQNRAEVDKVKALLSHDS GSDHLAFVRAVAGWEEVLRWQDRSSRENYLEENL LYAPSLRFIHGLIKQFSENIYEAFLVGKPSDCT LASAQCNEYSEEEELVKGVLMAGLYPNLIQVRQ GKVTRQGKFKPNSVTYRTKSGNILLHKSTINREA TRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAV LLLTDGDVHIRDDGRRATISLSDSDLLRLEGDS RTVRLLKELRRALGRMVERSLRSELAALPPSVQE EHGQLLALLAELLRGPCGSFDVRKTADD*ALLL LGLCTECKCLFKIKFYLSLV