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Sequence NM_001025193
GGGCTTAATGACGGAAGGAGCATGGCGTGGAGACACCTGAAAAAGCGGGCCCAGGATGCTGTGATCATCCTGGGGGGAGGAGGACTTCTCTTCGCCTCCTACCTGATGGCCACGGGAGATGAGCGTTTCTATGCTGAACACCTGATGCCGACTCTGCAGGGGCTGCTGGACCCGGAGTCAGCCCACAGACTGGCTGTTCG
CTTCACCTCCCTGGGGCTCCTTCCACGGGCCAGATTTCAAGACTCTGACATGCTGGAAGTGAGAGTTCTGGGCCATAAATTCCGAAATCCAGTAGGAATT
GCTGCAGGATTTGACAAGCATGGGGAAGCCGTGGACGGACTTTATAAGATGGGCTTTGGTTTTGTTGAGATAGGAAGTGTGACTCCAAAACCTCAGGAAG
GAAACCCTAGACCCAGAGTCTTCCGCCTCCCTGAGGACCAAGCTGTCATTAACAGGTATGGATTTAACAGTCACGGGCTTTCAGTGGTGGAACACAGGTT
ACGGGCCAGACAGCAGAAGCAGGCCAAGCTCACAGAAGTTGGGCATCGATGGGCTGATTGTTACGAACACCACCGTGAGTCGCCCTGCGGGCCTCCAGGG
TGCCCTGCGCTCTGAAACAGGAGGGCTGAGTGGGAAGCCCCTCCGGGATTTATCAACTCAAACCATTCGGGAGATGTATGCACTCACCCAAGGCCGAGTT
CCCATAATTGGGGTTGGTGGTGTGAGCAGCGGGCAGGACGCGCTGGAGAAGATCCGGGCAGGGGCCTCCCTGGTGCAGCTGTACACGGCCCTCACCTTCT
GGGGGCCACCCGTTGTGGGCAAAGTCAAGCGGGAACTGGAGGCCCTTCTGAAAGAGCAGGGCTTTGGCGGAGTCACAGATGCCATTGGAGCAGATCATCG
GAGGTGAGGACAGCGTCTGACGGGAAGCCTGATCTGGAACCTTCCCAAGGACTCAGGCAAGCCTTTGTGGCTGGATCATGAGAGGAGGGACTCCATCTTG
AGCCATGTCCCCCAGCCATGGCATGGCTGCACTGTAAACGCCAATCGGGGGGTCACCAGGATCAACCGCAGGCTTTCTTCAGTCCCTTGGTCAGACCATA
AACTGCATTTTTGATTCTTTGTGGATTCAAACCCTAGGATCCATCAGTCTTGCAAGGACATTGAATATTAGGAGGAAAAAGTCATGGAAAAAATAAAGCC
ATTTAGAACCTGGGTTTCAACGCTAGCCCTTTCTGGTTTGCCATAGGCCCTGCCAAGATACTGCAGGTCCATCCAGGCCTCTGCTATCTGCATCTGCAGT
GGGCTTCCCAGGAACTTGACTGTCTTTCATTTGATCTTTATTTTTGTTTATTTAATATTTTAAACTTTATTTTAAAAATATTTCAAACATAAGGGCGGGG
TGTGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGCCAGGAGTTGGAGACCAGCCAGGCCACCATGGTGAAA
CCCTGTCTCTACCAAAAATACAAAAAATTAGCCAGATATTGTGGCAGGCACTTTTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAACC
TGGGAGGCGGAGGTTGCAGTGAGCCAAGATTGCACCAGTGCACTCCAGCTTGGGCGACAGATCAAGACTCTGTCACACACAAAACAAACAAACAAATTTT
CCCGTTTCTCTCTGTCCCTTTCTCTATAACATGGATAAAATATTCACGTCTTATGTATTTATTA
- refseq_DHODH.F1 (forward primer)
- refseq_DHODH.R1 (reverse primer)
- Multiple exon skipping
Frame 0
GLNDGRSMAWRHLKKRAQDAVIILGGGGLLFASYLMATGDERFYAEHLMPTLQGLLDPESAHRLAVR
FTSLGLLPRARFQDSDMLEVRVLGHKFRNPVGI
AAGFDKHGEAVDGLYKMGFGFVEIGSVTPKPQE
GNPRPRVFRLPEDQAVINRYGFNSHGLSVVEHRL
RARQQKQAKLTEVGHRWADCYEHHRESPCGPPG
CPAL*NRRAEWEAPPGFINSNHSGDVCTHPRPS
SHNWGWWCEQRAGRAGEDPGRGLPGAAVHGPHLL
GATRCGQSQAGTGGPSERAGLWRSHRCHWSRSS
EVRTASDGKPDLEPSQGLRQAFVAGS*EEGLHL
EPCPPAMAWLHCKRQSGGHQDQPQAFFSPLVRP*
TAFLILCGFKP*DPSVLQGH*ILGGKSHGKNKA
I*NLGFNASPFWFAIGPAKILQVHPGLCYLHLQ
WASQELDCLSFDLYFCLFNILNFILKIFQT*GRG
VVAHACNPSTLGGRGGRIT*GQELETSQATMVK
PCLYQKYKKLARYCGRHF*SQLLGRLRQENCLN
LGGGGCSEPRLHQCTPAWATDQDSVTHKTNKQIF
PFLSVPFSITWIKYSRLMYLL
Frame 1
GLMTEGAWRGDT*KSGPRML*SSWGEEDFSSPPT*WPREMSVSMLNT*CRLCRGCWTRSQPTDWLF
ASPPWGSFHGPDFKTLTCWK*EFWAINSEIQ*EL
LQDLTSMGKPWTDFIRWALVLLR*EV*LQNLRK
ETLDPESSASLRTKLSLTGMDLTVTGFQWWNTG
YGPDSRSRPSSQKLGIDGLIVTNTTVSRPAGLQG
ALRSETGGLSGKPLRDLSTQTIREMYALTQGRV
PIIGVGGVSSGQDALEKIRAGASLVQLYTALTF
WGPPVVGKVKRELEALLKEQGFGGVTDAIGADHR
R*GQRLTGSLIWNLPKDSGKPLWLDHERRDSIL
SHVPQPWHGCTVNANRGVTRINRRLSSVPWSDH
KLHF*FFVDSNPRIHQSCKDIEY*EEKVMEKIKP
FRTWVSTLALSGLP*ALPRYCRSIQASAICICS
GLPRNLTVFHLIFIFVYLIF*TLF*KYFKHKGG
VWWLMPVIPALWEAEAGGSPEARSWRPARPPW*N
PVSTKNTKN*PDIVAGTFNPSYSGG*GRRIA*T
WEAEVAVSQDCTSALQLGRQIKTLSHTKQTNKF
SRFSLSLSL*HG*NIHVLCIY
Frame 2
A**RKEHGVETPEKAGPGCCDHPGGRRTSLRLLPDGHGR*AFLC*TPDADSAGAAGPGVSPQTGCS
LHLPGAPSTGQISRL*HAGSESSGP*IPKSSRN
CCRI*QAWGSRGRTL*DGLWFC*DRKCDSKTSGR
KP*TQSLPPP*GPSCH*QVWI*QSRAFSGGTQV
TGQTAEAGQAHRSWASMG*LLRTPP*VALRASR
VPCALKQEG*VGSPSGIYQLKPFGRCMHSPKAEF
P*LGLVV*AAGRTRWRRSGQGPPWCSCTRPSPS
GGHPLWAKSSGNWRPF*KSRALAESQMPLEQII
GGEDSV*REA*SGTFPRTQASLCGWIMRGGTPS*
AMSPSHGMAAL*TPIGGSPGSTAGFLQSLGQTI
NCIFDSLWIQTLGSISLARTLNIRRKKSWKK*S
HLEPGFQR*PFLVCHRPCQDTAGPSRPLLSASAV
GFPGT*LSFI*SLFLFI*YFKLYFKNISNIRAG
CGGSCL*SQHFGRPRRADHLRPGVGDQPGHHGE
TLSLPKIQKISQILWQALLIPATREAEAGELLEP
GRRRLQ*AKIAPVHSSLGDRSRLCHTQNKQTNF
PVSLCPFLYNMDKIFTSYVFI
Sequence NM_001361
GGGCTTAATGACGGAAGGAGCATGGCGTGGAGACACCTGAAAAAGCGGGCCCAGGATGCTGTGATCATCCTGGGGGGAGGAGGACTTCTCTTCGCCTCCTACCTGATGGCCACGGGAGATGAGCGTTTCTATGCTGAACACCTGATGCCGACTCTGCAGGGGCTGCTGGACCCGGAGTCAGCCCACAGACTGGCTGTTCG
CTTCACCTCCCTGGGGCTCCTTCCACGGGCCAGATTTCAAGACTCTGACATGCTGGAAGTGAGAGTTCTGGGCCATAAATTCCGAAATCCAGTAGGAATT
GCTGCAGGATTTGACAAGCATGGGGAAGCCGTGGACGGACTTTATAAGATGGGCTTTGGTTTTGTTGAGATAGGAAGTGTGACTCCAAAACCTCAGGAAG
GAAACCCTAGACCCAGAGTCTTCCGCCTCCCTGAGGACCAAGCTGTCATTAACAGGTATGGATTTAACAGTCACGGGCTTTCAGTGGTGGAACACAGGTT
ACGGGCCAGACAGCAGAAGCAGGCCAAGCTCACAGAAGATGGACTGCCTCTGGGGGTCAACTTGGGGAAGAACAAGACCTCAGTGGACGCCGCGGAGGAC
TACGCAGAAGGGGTGCGCGTACTGGGCCCCCTGGCCGACTACCTGGTGGTGAATGTGTCCAGCCCCAACACTGCCGGGCTGCGGAGCCTTCAGGGAAAGG
CCGAGCTGCGCCGCCTGCTGACCAAGGTGCTGCAGGAGAGGGATGGCTTGCGGAGAGTGCACAGGCCGGCAGTCCTGGTGAAGATCGCTCCTGACCTCAC
CAGCCAGGATAAGGAGGACATTGCCAGTGTGGTCAAAGAGTTGGGCATCGATGGGCTGATTGTTACGAACACCACCGTGAGTCGCCCTGCGGGCCTCCAG
GGTGCCCTGCGCTCTGAAACAGGAGGGCTGAGTGGGAAGCCCCTCCGGGATTTATCAACTCAAACCATTCGGGAGATGTATGCACTCACCCAAGGCCGAG
TTCCCATAATTGGGGTTGGTGGTGTGAGCAGCGGGCAGGACGCGCTGGAGAAGATCCGGGCAGGGGCCTCCCTGGTGCAGCTGTACACGGCCCTCACCTT
CTGGGGGCCACCCGTTGTGGGCAAAGTCAAGCGGGAACTGGAGGCCCTTCTGAAAGAGCAGGGCTTTGGCGGAGTCACAGATGCCATTGGAGCAGATCAT
CGGAGGTGAGGACAGCGTCTGACGGGAAGCCTGATCTGGAACCTTCCCAAGGACTCAGGCAAGCCTTTGTGGCTGGATCATGAGAGGAGGGACTCCATCT
TGAGCCATGTCCCCCAGCCATGGCATGGCTGCACTGTAAACGCCAATCGGGGGGTCACCAGGATCAACCGCAGGCTTTCTTCAGTCCCTTGGTCAGACCA
TAAACTGCATTTTTGATTCTTTGTGGATTCAAACCCTAGGATCCATCAGTCTTGCAAGGACATTGAATATTAGGAGGAAAAAGTCATGGAAAAAATAAAG
CCATTTAGAACCTGGGTTTCAACGCTAGCCCTTTCTGGTTTGCCATAGGCCCTGCCAAGATACTGCAGGTCCATCCAGGCCTCTGCTATCTGCATCTGCA
GTGGGCTTCCCAGGAACTTGACTGTCTTTCATTTGATCTTTATTTTTGTTTATTTAATATTTTAAACTTTATTTTAAAAATATTTCAAACATAAGGGCGG
GGTGTGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGCCAGGAGTTGGAGACCAGCCAGGCCACCATGGTGA
AACCCTGTCTCTACCAAAAATACAAAAAATTAGCCAGATATTGTGGCAGGCACTTTTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAA
CCTGGGAGGCGGAGGTTGCAGTGAGCCAAGATTGCACCAGTGCACTCCAGCTTGGGCGACAGATCAAGACTCTGTCACACACAAAACAAACAAACAAATT
TTCCCGTTTCTCTCTGTCCCTTTCTCTATAACATGGATAAAATATTCACGTCTTATGTATTTATTA
- refseq_DHODH.F1 (forward primer)
- refseq_DHODH.R1 (reverse primer)
- Multiple exon skipping
Frame 0
GLNDGRSMAWRHLKKRAQDAVIILGGGGLLFASYLMATGDERFYAEHLMPTLQGLLDPESAHRLAVR
FTSLGLLPRARFQDSDMLEVRVLGHKFRNPVGI
AAGFDKHGEAVDGLYKMGFGFVEIGSVTPKPQE
GNPRPRVFRLPEDQAVINRYGFNSHGLSVVEHRL
RARQQKQAKLTEDGLPLGVNLGKNKTSVDAAED
YAEGVRVLGPLADYLVVNVSSPNTAGLRSLQGK
AELRRLLTKVLQERDGLRRVHRPAVLVKIAPDLT
SQDKEDIASVVKELGIDGLIVTNTTVSRPAGLQ
GALRSETGGLSGKPLRDLSTQTIREMYALTQGR
VPIIGVGGVSSGQDALEKIRAGASLVQLYTALTF
WGPPVVGKVKRELEALLKEQGFGGVTDAIGADH
RR*GQRLTGSLIWNLPKDSGKPLWLDHERRDSI
LSHVPQPWHGCTVNANRGVTRINRRLSSVPWSDH
KLHF*FFVDSNPRIHQSCKDIEY*EEKVMEKIK
PFRTWVSTLALSGLP*ALPRYCRSIQASAICIC
SGLPRNLTVFHLIFIFVYLIF*TLF*KYFKHKGG
VWWLMPVIPALWEAEAGGSPEARSWRPARPPW*
NPVSTKNTKN*PDIVAGTFNPSYSGG*GRRIA*
TWEAEVAVSQDCTSALQLGRQIKTLSHTKQTNKF
SRFSLSLSL*HG*NIHVLCIY
Frame 1
GLMTEGAWRGDT*KSGPRML*SSWGEEDFSSPPT*WPREMSVSMLNT*CRLCRGCWTRSQPTDWLF
ASPPWGSFHGPDFKTLTCWK*EFWAINSEIQ*EL
LQDLTSMGKPWTDFIRWALVLLR*EV*LQNLRK
ETLDPESSASLRTKLSLTGMDLTVTGFQWWNTG
YGPDSRSRPSSQKMDCLWGSTWGRTRPQWTPRRT
TQKGCAYWAPWPTTWW*MCPAPTLPGCGAFRER
PSCAAC*PRCCRRGMACGECTGRQSW*RSLLTS
PARIRRTLPVWSKSWASMG*LLRTPP*VALRASR
VPCALKQEG*VGSPSGIYQLKPFGRCMHSPKAE
FP*LGLVV*AAGRTRWRRSGQGPPWCSCTRPSP
SGGHPLWAKSSGNWRPF*KSRALAESQMPLEQII
GGEDSV*REA*SGTFPRTQASLCGWIMRGGTPS
*AMSPSHGMAAL*TPIGGSPGSTAGFLQSLGQT
INCIFDSLWIQTLGSISLARTLNIRRKKSWKK*S
HLEPGFQR*PFLVCHRPCQDTAGPSRPLLSASA
VGFPGT*LSFI*SLFLFI*YFKLYFKNISNIRA
GCGGSCL*SQHFGRPRRADHLRPGVGDQPGHHGE
TLSLPKIQKISQILWQALLIPATREAEAGELLE
PGRRRLQ*AKIAPVHSSLGDRSRLCHTQNKQTN
FPVSLCPFLYNMDKIFTSYVFI
Frame 2
A**RKEHGVETPEKAGPGCCDHPGGRRTSLRLLPDGHGR*AFLC*TPDADSAGAAGPGVSPQTGCS
LHLPGAPSTGQISRL*HAGSESSGP*IPKSSRN
CCRI*QAWGSRGRTL*DGLWFC*DRKCDSKTSGR
KP*TQSLPPP*GPSCH*QVWI*QSRAFSGGTQV
TGQTAEAGQAHRRWTASGGQLGEEQDLSGRRGG
LRRRGARTGPPGRLPGGECVQPQHCRAAEPSGKG
RAAPPADQGAAGEGWLAESAQAGSPGEDRS*PH
QPG*GGHCQCGQRVGHRWADCYEHHRESPCGPP
GCPAL*NRRAEWEAPPGFINSNHSGDVCTHPRPS
SHNWGWWCEQRAGRAGEDPGRGLPGAAVHGPHL
LGATRCGQSQAGTGGPSERAGLWRSHRCHWSRS
SEVRTASDGKPDLEPSQGLRQAFVAGS*EEGLHL
EPCPPAMAWLHCKRQSGGHQDQPQAFFSPLVRP
*TAFLILCGFKP*DPSVLQGH*ILGGKSHGKNK
AI*NLGFNASPFWFAIGPAKILQVHPGLCYLHLQ
WASQELDCLSFDLYFCLFNILNFILKIFQT*GR
GVVAHACNPSTLGGRGGRIT*GQELETSQATMV
KPCLYQKYKKLARYCGRHF*SQLLGRLRQENCLN
LGGGGCSEPRLHQCTPAWATDQDSVTHKTNKQI
FPFLSVPFSITWIKYSRLMYLL
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