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Sequences

Sequence NM_001033086

CGGCGAGCAGCGCAGGACGCAGAGCCTCTTTCACTTTTTCCCTGCTGAGTGCCCCCTCCCACCCCTCCCACTCCACACACACCCTGTTTGCCCGTGAGCC TGGGGAACTTGCAGCTTAAAGCCAGCCACCCCCACGGCAACATGTACCCCAGCAACAAGAAGAAAAAGGTGTGGAGAGAGGAGAAAGAACGTTTATTGAA GATGACCTTAGAAGAGAGACGCAAAGAATACCTAAGAGACTATATTCCCCTGAACAGCATTCTATCATGGAAGGAGGAGATGAAGGGCAAGGGCCAAAAT GATGAAGAAAATACTCAGGAAACATCCCAGGTGAAGAAAAGTTTGACTGAAAAAGTTTCTCTCTATAGAGGTGACATCACATTGCTAGAGGTAGATGCTA TAGTCAATGCCGCAAATGCCAGTCTTCTTGGAGGAGGAGGTGTGGATGGCTGTATTCATAGAGCAGCCGGCCCCTGTTTGCTAGCTGAATGTCGTAACCT GAATGGCTGTGATACTGGACATGCAAAAATCACATGTGGCTATGACCTTCCTGCAAAATATGTCATCCATACTGTAGGGCCAATAGCCAGGGGCCATATT AATGGTTCCCACAAGGAAGACCTTGCAAATTGCTATAAATCATCTCTGAAGCTCGTGAAAGAAAATAACATCCGATCAGTTGCATTTCCCTGCATCTCAA CAGGCATTTATGGCTTTCCCAACGAGCCTGCTGCAGTCATTGCCCTCAACACCATTAAGGAATGGCTTGCCAAGAATCACCATGAGGTGGATCGGATCAT TTTCTGTGTCTTCTTAGAAGTTGACTTCAAAATCTACAAAAAGAAAATGAATGAGTTTTTCTCCGTAGACGATAATAATGAAGAAGAAGAGGATGTTGAA ATGAAAGAAGATTCAGATGAGAACGGTCCAGAGGAGAAGCAAAGTGTGGAAGAAATGGAAGAGCAGAGCCAAGATGCAGATGGTGTCAACACTGTCACTG TGCCCGGCCCTGCTTCAGAAGAGGCAGTTGAAGACTGTAAAGATGAAGATTTTGCAAAGGATGAAAATATTACAAAAGGCGGTGAAGTGACAGATCATTC TGTGCGTGACCAAGATCATCCCGATGGACAAGAGAATGATTCAACGAAGAATGAAATAAAAATTGAAACAGAATCGCAGAGCTCATATATGGAAACAGAA GAACTTTCATCAAACCAAGAAGATGCCGTGATTGTGGAGCAACCAGAAGTGATTCCATTAACAGAGGACCAAGAAGAAAAAGAAGGTGAAAAAGCTCCAG GCGAGGACACACCTAGGATGCCTGGGAAAAGTGAAGGCTCCAGTGACCTAGAAAATACTCCAGGTCCTGATGCAGGGGCACAAGATGAAGCGAAGGAACA AAGAAATGGAACTAAATGACAATCCTCAGCATCGCAAGGCCTCTCCTGGCTCTGGGGGAGCTCGGGAAGATAGCAGCACACGCTGTGGAGGAGGGTGGGG GTGGGGGGAAGGCAAGTCCCATGGAAGGACGGGGAATCCTTTACTCTAATTTCTCCAGCTGCATTTTGTTCCGTTTATCTGCAGAAAAAGAAAGAAAAAA AAGAAAAAAAAAGTTTCCTTTAATTTGGTGGAGGGACCCATGTTGACGCATCTTTCAGGCATTATCCTTGTAATTTCTGTCTTTTCTCTTACAACTTTGC CCCAGGGTCACAGTGGCTTGATTGAACACTCACATGTGTATCCTGGCCCCTGTCTGCTTTCTTGGTTATTTCACAAAGCTGGTCACACAGTGGTTTATTC AAAGGAAGGGGAGGAAGACAGTGGTTTGATAAGCTGCAGGATAAATTTTAGGAATCAATGAGCCCAGCAGCAGTATAATCCCCAGACAGAGGAGGCAGGA TAGAAAATGGGCAAAAGCCTCGGAAACCACTTGGAAAAGGTCTGGACAATGAGGTGAAAATATTTTCTTCAGGGTTCCCAAGGCACAATTTGTTCCAAGT GGCTAATGAGAAATATGGAAGCTGAATTTTTTCCAGAGCAGAGTGCAGAGGCATAACAGAAGGGTGGGCCCTGGCAGCCATCTGGGTCTCTTCCTTCCTA ACCATGGTGGCAGGTGCATCCTTCTTTGACACTGACTTTCAGCAGAGCTTACTTGGTTCATGAGGTCTTCACATGGAGACTACCAGCAAGAGGTGACTCT CTGCTGCATAACTGTAAAGGATGGCCCTTTGCTAGGTGTTACAGTTAAAAGCTAAGAAAAGGGGCAGTGCATTTAGGACCCAAACATATGCCTATGAATA TCAAAAGCTCCTCCTGAAATTGCTGTGAGTTTTCCATAAAAGAATATCCTGTCTTCACCCAAGGCTTGACAGCCCACAGAGTGGTCTCATTTGAAATTAC AGGAAATTAGAGCTTTTGCTTGCAGTTCTGCCTTCCTGGCCTGTGTTTAAATGCTGTCACTTGTTTATGCCAAGTTCAAGGCTGATTCAATGGTTGGTCC CCTCACCCAGAAAACCCTGAAGGGGAGGATACAGCTCTGAAGGGGGGCAGCAGTACTAAAAACCCAAGATGCCAGTGGTATAGTGGGCACAAGGGATGGC GACCATGAGGATGCCAGGCATCATCACCAATATCTATCCTAGAGCCAGTATAAGGCCAGATGCCTACTTCCCACAGCCTCCCCGGGTTCCAAAGTCATGT CATTGTTTTCAGTGGAAACATATCGTTTGTTGCATATCTTCTTAAATCCATCTTCCTTGTAAGGGCTTTAGAACTAAAACTTACTTATATTGTTTTTCCT TAACAGAGGGAGAAAAATAGTGGATTATTATTTCTAAAATAAAAGGATGTTCTGCTTTCTAAATATCCCATCAAAATCTTCAGTTTTGCACTTTTTTGAT GGAAAATTCATCTTATCTTCCTATGACTTTGGTTTTAGCCTTTCTGAATTTGTTACCCCTTCTGGATGGCTTATTTGATATACTGGAATAGTTAACAAGC TATACTTCAGCATATGCACTATATTCTAACAAATTTTTTTTAATAAAATCAAGACATCAGCAAGAATGACATTTACGTGACCTCATAATGTGGGATTATG GCCTTCTGTTGCTATTCCAGTTTGATATGGAAGCATCTATATCCTCTATTGCCGTTAGATGTTGTTGCTTTTCAGAAAAGTAACGAAAAGGCTCATTTTA AAGAATCCAAGAAACGATGTCATCCAAATATTGACAGTTTCTACATTTCATGCCATCTTTATAACTCAATTGAAAGTTGCCGTCATTCTTGTGAAGTATT TGACAAGTGCAATCTGCTAGAAGCTCGTTTTTCTTGTGACTCCCAAATGTTAGTGCTACTTAGCCTCAGTAATGAGTTACAGTTGAAAAAAACATGAGGG AAACAGAGGGACAGAGATTTTCTAATGAACAATGATGGAAGAGACCTAATGTCCTTGCTAGAAACAGCCAGGATGGAAATTATCCAGCCCTGGCATTCTC CTTATCATCAATGACAGTCATTTTATTCATTTATTTCAAATGTGGGTGGGCTAGAAGTGGAAGGAGGGAATTCTCTCTGCCTAAAAATTCTAGAAGAATG AAAGTAATCTTTGTATCCAGGAAACTAAGAGAATGAGGAATAAATATCTTCAGCCCGACTCCTGAATTTGTTTATTCTTCCATCTATAATTAGATTGTGT TTTCATTTTTGCTTTGTCATGCTTTTTGGTTGTTATTTGGCTATACAGTTTTATGCTTTAAAACAAATGATAAAGTTAATTTCCAATTCAATAGTGAAAT ATTAACAATCTAACTATAGCCAGATCAAAGACACCTGAACACAGAAAACCTTTATTTGCTGGTGCTGCCATTGCACAGGCTGTACAATGAAATAGATTTG AAAAGCTGATTGATTTTCCCGCACATAAATTCTGGATGTCAATTTCCAACCAAACTCCAATACAGCTATGTGGCATGAAGAGTTACAGGAGGGAGGGAGG AAAATAGCCCTATGTTAGTCATGTTTGCATACAGAGGATCAAAGTAGGCCTTCACCATAATAGTTCTAATTAAAATGGTCCTCGCTGTAGGAGAGACAAA GGGGCTTTTCCTCTAGCTGGTAACTATTCAGATGATGGACAAGTCTTCTTTCATAAAAGATTACAAAGAAGGCATCCGAATCACTGTCTGTGATACTGGG TCACATATTAATCACTGCAGCTAATTGTAAATCTTTCTATGAAACACTGAAAAGCCTCTTTGTGAATTAATACAGTTCTGCTTGATGCACTTGATTTGAA AAGACATTTCTCTGTATGTGGCGCATGTCGGCTTTGCTTTGAAAAATAACAAAGTTAGCAGAATATGTTCAATATATTTTCTTGGGGAATAGGGTTTTTA TTACATGATTCATTAAGGATTTGCCTTACCCTGACATTTGTGATATAAAGGAAAATCAGAAAAAAAGTAATTTTCTTGATCAAGATATGTTTTTACTTAA TGCAAATAAATGTAGTCTGTTGCTTGCAAGGAAAAAAAAATGGCTTCTGATATCTGGTATAAACTGCTAAATAGGATAATACGTGCCTCTTTTGTTAAAC CGGCATTTAAATGCTGGACTGCTTCTAAATCTGTTTGTTTCTTTTCATCTGTGCCATACACTAAAAAACAACTGTTGCCTTCATACTATATTTGTTAGAG CAGAATACAAATAAAATTTGTTTGAGAGGATAATGTGAAA

refseq_C20orf133.F2 (forward primer)
refseq_C20orf133.R2 (reverse primer)
Single exon skipping

Frame 0

RRAAQDAEPLSLFPC*VPPPTPPTPHTPCLPVS LGNLQLKASHPHGNMYPSNKKKKVWREEKERLLK MTLEERRKEYLRDYIPLNSILSWKEEMKGKGQN DEENTQETSQVKKSLTEKVSLYRGDITLLEVDA IVNAANASLLGGGGVDGCIHRAAGPCLLAECRNL NGCDTGHAKITCGYDLPAKYVIHTVGPIARGHI NGSHKEDLANCYKSSLKLVKENNIRSVAFPCIS TGIYGFPNEPAAVIALNTIKEWLAKNHHEVDRII FCVFLEVDFKIYKKKMNEFFSVDDNNEEEEDVE MKEDSDENGPEEKQSVEEMEEQSQDADGVNTVT VPGPASEEAVEDCKDEDFAKDENITKGGEVTDHS VRDQDHPDGQENDSTKNEIKIETESQSSYMETE ELSSNQEDAVIVEQPEVIPLTEDQEEKEGEKAP GEDTPRMPGKSEGSSDLENTPGPDAGAQDEAKEQ RNGTK*QSSASQGLSWLWGSSGR*QHTLWRRVG VGGRQVPWKDGESFTLISPAAFCSVYLQKKKEK KEKKSFL*FGGGTHVDASFRHYPCNFCLFSYNFA PGSQWLD*TLTCVSWPLSAFLVISQSWSHSGLF KGRGGRQWFDKLQDKF*ESMSPAAV*SPDRGGR IENGQKPRKPLGKGLDNEVKIFSSGFPRHNLFQV ANEKYGS*IFSRAECRGITEGWALAAIWVSSFL TMVAGASFFDTDFQQSLLGS*GLHMETTSKR*L SAA*L*RMALC*VLQLKAKKRGSAFRTQTYAYEY QKLLLKLL*VFHKRISCLHPRLDSPQSGLI*NY RKLELLLAVLPSWPVFKCCHLFMPSSRLIQWLV PSPRKP*RGGYSSEGGQQY*KPKMPVV*WAQGMA TMRMPGIITNIYPRASIRPDAYFPQPPRVPKSC HCFQWKHIVCCISS*IHLPCKGFRTKTYLYCFS LTEGEK*WIIISKIKGCSAF*ISHQNLQFCTFLM ENSSYLPMTLVLAFLNLLPLLDGLFDILE*LTS YTSAYALYSNKFFLIKSRHQQE*HLRDLIMWDY GLLLLFQFDMEASISSIAVRCCCFSEK*RKGSF* RIQETMSSKY*QFLHFMPSL*LN*KLPSFL*SI *QVQSARSSFFL*LPNVSAT*PQ**VTVEKNMR ETEGQRFSNEQ*WKRPNVLARNSQDGNYPALAFS LSSMTVILFIYFKCGWARSGRREFSLPKNSRRM KVIFVSRKLRE*GINIFSPTPEFVYSSIYN*IV FSFLLCHAFWLLFGYTVLCFKTNDKVNFQFNSEI LTI*L*PDQRHLNTENLYLLVLPLHRLYNEIDL KS*LIFPHINSGCQFPTKLQYSYVA*RVTGGRE ENSPMLVMFAYRGSK*AFTIIVLIKMVLAVGETK GLFL*LVTIQMMDKSSFIKDYKEGIRITVCDTG SHINHCS*L*IFL*NTEKPLCELIQFCLMHLI* KDISLYVAHVGFALKNNKVSRICSIYFLGE*GFY YMIH*GFALP*HL*YKGKSEKK*FS*SRYVFT* CK*M*SVACKEKKMASDIWYKLLNRIIRASFVK PAFKCWTASKSVCFFSSVPYTKKQLLPSYYIC*S RIQIKFV*EDNVK

Frame 1

GEQRRTQSLFHFFPAECPLPPLPLHTHPVCP*A WGTCSLKPATPTATCTPATRRKRCGERRKNVY* R*P*KRDAKNT*ETIFP*TAFYHGRRR*RARAKM MKKILRKHPR*RKV*LKKFLSIEVTSHC*R*ML *SMPQMPVFLEEEVWMAVFIEQPAPVC*LNVVT *MAVILDMQKSHVAMTFLQNMSSIL*GQ*PGAIL MVPTRKTLQIAINHL*SS*KKITSDQLHFPASQ QAFMAFPTSLLQSLPSTPLRNGLPRITMRWIGS FSVSS*KLTSKSTKRK*MSFSP*TIIMKKKRMLK *KKIQMRTVQRRSKVWKKWKSRAKMQMVSTLSL CPALLQKRQLKTVKMKILQRMKILQKAVK*QII LCVTKIIPMDKRMIQRRMK*KLKQNRRAHIWKQK NFHQTKKMP*LWSNQK*FH*QRTKKKKKVKKLQ ARTHLGCLGKVKAPVT*KILQVLMQGHKMKRRN KEMELNDNPQHRKASPGSGGAREDSSTRCGGGWG WGEGKSHGRTGNPLL*FLQLHFVPFICRKRKKK KKKKVSFNLVEGPMLTHLSGIILVISVFSLTTL PQGHSGLIEHSHVYPGPCLLSWLFHKAGHTVVYS KEGEEDSGLISCRINFRNQ*AQQQYNPQTEEAG *KMGKSLGNHLEKVWTMR*KYFLQGSQGTICSK WLMRNMEAEFFPEQSAEA*QKGGPWQPSGSLPS* PWWQVHPSLTLTFSRAYLVHEVFTWRLPARGDS LLHNCKGWPFARCYS*KLRKGAVHLGPKHMPMN IKSSS*NCCEFSIKEYPVFTQGLTAHRVVSFEIT GN*SFCLQFCLPGLCLNAVTCLCQVQG*FNGWS PHPENPEGEDTALKGGSSTKNPRCQWYSGHKGW RP*GCQASSPISILEPV*GQMPTSHSLPGFQSHV IVFSGNISFVAYLLKSIFLVRALELKLTYIVFP *QREKNSGLLFLK*KDVLLSKYPIKIFSFALF* WKIHLIFL*LWF*PF*ICYPFWMAYLIYWNS*QA ILQHMHYILTNFF**NQDISKNDIYVTS*CGIM AFCCYSSLIWKHLYPLLPLDVVAFQKSNEKAHF KESKKRCHPNIDSFYISCHLYNSIESCRHSCEVF DKCNLLEARFSCDSQMLVLLSLSNELQLKKT*G KQRDRDFLMNNDGRDLMSLLETARMEIIQPWHS PYHQ*QSFYSFISNVGGLEVEGGNSLCLKILEE* K*SLYPGN*ENEE*ISSARLLNLFILPSIIRLC FHFCFVMLFGCYLAIQFYALKQMIKLISNSIVK Y*QSNYSQIKDT*TQKTFICWCCHCTGCTMK*I* KAD*FSRT*ILDVNFQPNSNTAMWHEELQEGGR KIALC*SCLHTEDQSRPSP**F*LKWSSL*ERQ RGFSSSW*LFR*WTSLLS*KITKKASESLSVILG HILITAANCKSFYETLKSLFVN*YSSA*CT*FE KTFLCMWRMSALL*KITKLAEYVQYIFLGNRVF IT*FIKDLPYPDICDIKENQKKSNFLDQDMFLLN ANKCSLLLARKKKWLLISGINC*IG*YVPLLLN RHLNAGLLLNLFVSFHLCHTLKNNCCLHTIFVR AEYK*NLFERIM*

Frame 2

ASSAGRRASFTFSLLSAPSHPSHSTHTLFAREP GELAA*SQPPPRQHVPQQQEEKGVERGERTFIE DDLRRETQRIPKRLYSPEQHSIMEGGDEGQGPK **RKYSGNIPGEEKFD*KSFSL*R*HHIARGRCY SQCRKCQSSWRRRCGWLYS*SSRPLFAS*MS*P EWL*YWTCKNHMWL*PSCKICHPYCRANSQGPY *WFPQGRPCKLL*IISEARERK*HPISCISLHLN RHLWLSQRACCSHCPQHH*GMACQESP*GGSDH FLCLLRS*LQNLQKENE*VFLRRR***RRRGC* NERRFR*ERSRGEAKCGRNGRAEPRCRWCQHCHC ARPCFRRGS*RL*R*RFCKG*KYYKRR*SDRSF CA*PRSSRWTRE*FNEE*NKN*NRIAELIYGNR RTFIKPRRCRDCGATRSDSINRGPRRKRR*KSSR RGHT*DAWEK*RLQ*PRKYSRS*CRGTR*SEGT KKWN*MTILSIARPLLALGELGKIAAHAVEEGG GGGKASPMEGRGILYSNFSSCILFRLSAEKERKK RKKKFPLIWWRDPC*RIFQALSL*FLSFLLQLC PRVTVA*LNTHMCILAPVCFLGYFTKLVTQWFI QRKGRKTVV**AAG*ILGINEPSSSIIPRQRRQD RKWAKASETTWKRSGQ*GENIFFRVPKAQFVPS G**EIWKLNFFQSRVQRHNRRVGPGSHLGLFLP NHGGRCILL*H*LSAELTWFMRSSHGDYQQEVTL CCITVKDGPLLGVTVKS*EKGQCI*DPNICL*I SKAPPEIAVSFP*KNILSSPKA*QPTEWSHLKL QEIRAFACSSAFLACV*MLSLVYAKFKADSMVGP LTQKTLKGRIQL*RGAAVLKTQDASGIVGTRDG DHEDARHHHQYLS*SQYKARCLLPTASPGSKVM SLFSVETYRLLHIFLNPSSL*GL*N*NLLILFFL NRGRKIVDYYF*NKRMFCFLNIPSKSSVLHFFD GKFILSSYDFGFSLSEFVTPSGWLI*YTGIVNK LYFSICTIF*QIFFNKIKTSARMTFT*PHNVGLW PSVAIPV*YGSIYILYCR*MLLLFRKVTKRLIL KNPRNDVIQILTVSTFHAIFITQLKVAVILVKY LTSAIC*KLVFLVTPKC*CYLASVMSYS*KKHEG NRGTEIF**TMMEET*CPC*KQPGWKLSSPGIL LIINDSHFIHLFQMWVG*KWKEGILSA*KF*KN ESNLCIQETKRMRNKYLQPDS*ICLFFHL*LDCV FIFALSCFLVVIWLYSFML*NK**S*FPIQ**N INNLTIARSKTPEHRKPLFAGAAIAQAVQ*NRF EKLIDFPAHKFWMSISNQTPIQLCGMKSYRREGG K*PYVSHVCIQRIKVGLHHNSSN*NGPRCRRDK GAFPLAGNYSDDGQVFFHKRLQRRHPNHCL*YW VTY*SLQLIVNLSMKH*KASL*INTVLLDALDLK RHFSVCGACRLCFEK*QS*QNMFNIFSWGIGFL LHDSLRICLTLTFVI*RKIRKKVIFLIKICFYL MQINVVCCLQGKKNGF*YLV*TAK*DNTCLFC*T GI*MLDCF*ICLFLFICAIH*KTTVAFILYLLE QNTNKICLRG*CE

Sequence NM_001033087

ACTATCGAGAAGCAGATGACAGGCTCAGGCCTTACCAGGGATGCACTTGCCACATTTTGGAAAGACTACATAAAAAACCTTTAAAAATTGGAGCCCTTTG GGCCTCAGGTATCCAAAGAGCACATTTTTTGGAAACTCAACACTCAGGAAGGTCGCATAGAGCCAGTTTATGCAGATGTCATCCATACTGTAGGGCCAAT AGCCAGGGGCCATATTAATGGTTCCCACAAGGAAGACCTTGCAAATTGCTATAAATCATCTCTGAAGCTCGTGAAAGAAAATAACATCCGATCAGTTGCA TTTCCCTGCATCTCAACAGGCATTTATGGCTTTCCCAACGAGCCTGCTGCAGTCATTGCCCTCAACACCATTAAGGAATGGCTTGCCAAGAATCACCATG AGGTGGATCGGATCATTTTCTGTGTCTTCTTAGAAGTTGACTTCAAAATCTACAAAAAGAAAATGAATGAGTTTTTCTCCGTAGACGATAATAATGAAGA AGAAGAGGATGTTGAAATGAAAGAAGATTCAGATGAGAACGGTCCAGAGGAGAAGCAAAGTGTGGAAGAAATGGAAGAGCAGAGCCAAGATGCAGATGGT GTCAACACTGTCACTGTGCCCGGCCCTGCTTCAGAAGAGGCAGTTGAAGACTGTAAAGATGAAGATTTTGCAAAGGATGAAAATATTACAAAAGGCGGTG AAGTGACAGATCATTCTGTGCGTGACCAAGATCATCCCGATGGACAAGAGAATGATTCAACGAAGAATGAAATAAAAATTGAAACAGAATCGCAGAGCTC ATATATGGAAACAGAAGAACTTTCATCAAACCAAGAAGATGCCGTGATTGTGGAGCAACCAGAAGTGATTCCATTAACAGAGGACCAAGAAGAAAAAGAA GGTGAAAAAGCTCCAGGCGAGGACACACCTAGGATGCCTGGGAAAAGTGAAGGCTCCAGTGACCTAGAAAATACTCCAGGTCCTGATGTTGAAATGAATA GTCAGGTTGACAAGGTAAATGACCCAACAGAGAGTCAACAAGAAGATCAACTAATAGCAGGGGCACAAGATGAAGCGAAGGAACAAAGAAATGGAACTAA ATGACAATCCTCAGCATCGCAAGGCCTCTCCTGGCTCTGGGGGAGCTCGGGAAGATAGCAGCACACGCTGTGGAGGAGGGTGGGGGTGGGGGGAAGGCAA GTCCCATGGAAGGACGGGGAATCCTTTACTCTAATTTCTCCAGCTGCATTTTGTTCCGTTTATCTGCAGAAAAAGAAAGAAAAAAAAGAAAAAAAAAGTT TCCTTTAATTTGGTGGAGGGACCCATGTTGACGCATCTTTCAGGCATTATCCTTGTAATTTCTGTCTTTTCTCTTACAACTTTGCCCCAGGGTCACAGTG GCTTGATTGAACACTCACATGTGTATCCTGGCCCCTGTCTGCTTTCTTGGTTATTTCACAAAGCTGGTCACACAGTGGTTTATTCAAAGGAAGGGGAGGA AGACAGTGGTTTGATAAGCTGCAGGATAAATTTTAGGAATCAATGAGCCCAGCAGCAGTATAATCCCCAGACAGAGGAGGCAGGATAGAAAATGGGCAAA AGCCTCGGAAACCACTTGGAAAAGGTCTGGACAATGAGGTGAAAATATTTTCTTCAGGGTTCCCAAGGCACAATTTGTTCCAAGTGGCTAATGAGAAATA TGGAAGCTGAATTTTTTCCAGAGCAGAGTGCAGAGGCATAACAGAAGGGTGGGCCCTGGCAGCCATCTGGGTCTCTTCCTTCCTAACCATGGTGGCAGGT GCATCCTTCTTTGACACTGACTTTCAGCAGAGCTTACTTGGTTCATGAGGTCTTCACATGGAGACTACCAGCAAGAGGTGACTCTCTGCTGCATAACTGT AAAGGATGGCCCTTTGCTAGGTGTTACAGTTAAAAGCTAAGAAAAGGGGCAGTGCATTTAGGACCCAAACATATGCCTATGAATATCAAAAGCTCCTCCT GAAATTGCTGTGAGTTTTCCATAAAAGAATATCCTGTCTTCACCCAAGGCTTGACAGCCCACAGAGTGGTCTCATTTGAAATTACAGGAAATTAGAGCTT TTGCTTGCAGTTCTGCCTTCCTGGCCTGTGTTTAAATGCTGTCACTTGTTTATGCCAAGTTCAAGGCTGATTCAATGGTTGGTCCCCTCACCCAGAAAAC CCTGAAGGGGAGGATACAGCTCTGAAGGGGGGCAGCAGTACTAAAAACCCAAGATGCCAGTGGTATAGTGGGCACAAGGGATGGCGACCATGAGGATGCC AGGCATCATCACCAATATCTATCCTAGAGCCAGTATAAGGCCAGATGCCTACTTCCCACAGCCTCCCCGGGTTCCAAAGTCATGTCATTGTTTTCAGTGG AAACATATCGTTTGTTGCATATCTTCTTAAATCCATCTTCCTTGTAAGGGCTTTAGAACTAAAACTTACTTATATTGTTTTTCCTTAACAGAGGGAGAAA AATAGTGGATTATTATTTCTAAAATAAAAGGATGTTCTGCTTTCTAAATATCCCATCAAAATCTTCAGTTTTGCACTTTTTTGATGGAAAATTCATCTTA TCTTCCTATGACTTTGGTTTTAGCCTTTCTGAATTTGTTACCCCTTCTGGATGGCTTATTTGATATACTGGAATAGTTAACAAGCTATACTTCAGCATAT GCACTATATTCTAACAAATTTTTTTTAATAAAATCAAGACATCAGCAAGAATGACATTTACGTGACCTCATAATGTGGGATTATGGCCTTCTGTTGCTAT TCCAGTTTGATATGGAAGCATCTATATCCTCTATTGCCGTTAGATGTTGTTGCTTTTCAGAAAAGTAACGAAAAGGCTCATTTTAAAGAATCCAAGAAAC GATGTCATCCAAATATTGACAGTTTCTACATTTCATGCCATCTTTATAACTCAATTGAAAGTTGCCGTCATTCTTGTGAAGTATTTGACAAGTGCAATCT GCTAGAAGCTCGTTTTTCTTGTGACTCCCAAATGTTAGTGCTACTTAGCCTCAGTAATGAGTTACAGTTGAAAAAAACATGAGGGAAACAGAGGGACAGA GATTTTCTAATGAACAATGATGGAAGAGACCTAATGTCCTTGCTAGAAACAGCCAGGATGGAAATTATCCAGCCCTGGCATTCTCCTTATCATCAATGAC AGTCATTTTATTCATTTATTTCAAATGTGGGTGGGCTAGAAGTGGAAGGAGGGAATTCTCTCTGCCTAAAAATTCTAGAAGAATGAAAGTAATCTTTGTA TCCAGGAAACTAAGAGAATGAGGAATAAATATCTTCAGCCCGACTCCTGAATTTGTTTATTCTTCCATCTATAATTAGATTGTGTTTTCATTTTTGCTTT GTCATGCTTTTTGGTTGTTATTTGGCTATACAGTTTTATGCTTTAAAACAAATGATAAAGTTAATTTCCAATTCAATAGTGAAATATTAACAATCTAACT ATAGCCAGATCAAAGACACCTGAACACAGAAAACCTTTATTTGCTGGTGCTGCCATTGCACAGGCTGTACAATGAAATAGATTTGAAAAGCTGATTGATT TTCCCGCACATAAATTCTGGATGTCAATTTCCAACCAAACTCCAATACAGCTATGTGGCATGAAGAGTTACAGGAGGGAGGGAGGAAAATAGCCCTATGT TAGTCATGTTTGCATACAGAGGATCAAAGTAGGCCTTCACCATAATAGTTCTAATTAAAATGGTCCTCGCTGTAGGAGAGACAAAGGGGCTTTTCCTCTA GCTGGTAACTATTCAGATGATGGACAAGTCTTCTTTCATAAAAGATTACAAAGAAGGCATCCGAATCACTGTCTGTGATACTGGGTCACATATTAATCAC TGCAGCTAATTGTAAATCTTTCTATGAAACACTGAAAAGCCTCTTTGTGAATTAATACAGTTCTGCTTGATGCACTTGATTTGAAAAGACATTTCTCTGT ATGTGGCGCATGTCGGCTTTGCTTTGAAAAATAACAAAGTTAGCAGAATATGTTCAATATATTTTCTTGGGGAATAGGGTTTTTATTACATGATTCATTA AGGATTTGCCTTACCCTGACATTTGTGATATAAAGGAAAATCAGAAAAAAAGTAATTTTCTTGATCAAGATATGTTTTTACTTAATGCAAATAAATGTAG TCTGTTGCTTGCAAGGAAAAAAAAATGGCTTCTGATATCTGGTATAAACTGCTAAATAGGATAATACGTGCCTCTTTTGTTAAACCGGCATTTAAATGCT GGACTGCTTCTAAATCTGTTTGTTTCTTTTCATCTGTGCCATACACTAAAAAACAACTGTTGCCTTCATACTATATTTGTTAGAGCAGAATACAAATAAA ATTTGTTTGAGAGGATAATGTGAAA

refseq_C20orf133.F2 (forward primer)
refseq_C20orf133.R2 (reverse primer)
Single exon skipping

Frame 0

TIEKQMTGSGLTRDALATFWKDYIKNL*KLEPF GPQVSKEHIFWKLNTQEGRIEPVYADVIHTVGPI ARGHINGSHKEDLANCYKSSLKLVKENNIRSVA FPCISTGIYGFPNEPAAVIALNTIKEWLAKNHH EVDRIIFCVFLEVDFKIYKKKMNEFFSVDDNNEE EEDVEMKEDSDENGPEEKQSVEEMEEQSQDADG VNTVTVPGPASEEAVEDCKDEDFAKDENITKGG EVTDHSVRDQDHPDGQENDSTKNEIKIETESQSS YMETEELSSNQEDAVIVEQPEVIPLTEDQEEKE GEKAPGEDTPRMPGKSEGSSDLENTPGPDVEMN SQVDKVNDPTESQQEDQLIAGAQDEAKEQRNGTK *QSSASQGLSWLWGSSGR*QHTLWRRVGVGGRQ VPWKDGESFTLISPAAFCSVYLQKKKEKKEKKS FL*FGGGTHVDASFRHYPCNFCLFSYNFAPGSQW LD*TLTCVSWPLSAFLVISQSWSHSGLFKGRGG RQWFDKLQDKF*ESMSPAAV*SPDRGGRIENGQ KPRKPLGKGLDNEVKIFSSGFPRHNLFQVANEKY GS*IFSRAECRGITEGWALAAIWVSSFLTMVAG ASFFDTDFQQSLLGS*GLHMETTSKR*LSAA*L *RMALC*VLQLKAKKRGSAFRTQTYAYEYQKLLL KLL*VFHKRISCLHPRLDSPQSGLI*NYRKLEL LLAVLPSWPVFKCCHLFMPSSRLIQWLVPSPRK P*RGGYSSEGGQQY*KPKMPVV*WAQGMATMRMP GIITNIYPRASIRPDAYFPQPPRVPKSCHCFQW KHIVCCISS*IHLPCKGFRTKTYLYCFSLTEGE K*WIIISKIKGCSAF*ISHQNLQFCTFLMENSSY LPMTLVLAFLNLLPLLDGLFDILE*LTSYTSAY ALYSNKFFLIKSRHQQE*HLRDLIMWDYGLLLL FQFDMEASISSIAVRCCCFSEK*RKGSF*RIQET MSSKY*QFLHFMPSL*LN*KLPSFL*SI*QVQS ARSSFFL*LPNVSAT*PQ**VTVEKNMRETEGQ RFSNEQ*WKRPNVLARNSQDGNYPALAFSLSSMT VILFIYFKCGWARSGRREFSLPKNSRRMKVIFV SRKLRE*GINIFSPTPEFVYSSIYN*IVFSFLL CHAFWLLFGYTVLCFKTNDKVNFQFNSEILTI*L *PDQRHLNTENLYLLVLPLHRLYNEIDLKS*LI FPHINSGCQFPTKLQYSYVA*RVTGGREENSPM LVMFAYRGSK*AFTIIVLIKMVLAVGETKGLFL* LVTIQMMDKSSFIKDYKEGIRITVCDTGSHINH CS*L*IFL*NTEKPLCELIQFCLMHLI*KDISL YVAHVGFALKNNKVSRICSIYFLGE*GFYYMIH* GFALP*HL*YKGKSEKK*FS*SRYVFT*CK*M* SVACKEKKMASDIWYKLLNRIIRASFVKPAFKC WTASKSVCFFSSVPYTKKQLLPSYYIC*SRIQIK FV*EDNVK

Frame 1

LSRSR*QAQALPGMHLPHFGKTT*KTFKNWSPL GLRYPKSTFFGNSTLRKVA*SQFMQMSSIL*GQ *PGAILMVPTRKTLQIAINHL*SS*KKITSDQLH FPASQQAFMAFPTSLLQSLPSTPLRNGLPRITM RWIGSFSVSS*KLTSKSTKRK*MSFSP*TIIMK KKRMLK*KKIQMRTVQRRSKVWKKWKSRAKMQMV STLSLCPALLQKRQLKTVKMKILQRMKILQKAV K*QIILCVTKIIPMDKRMIQRRMK*KLKQNRRA HIWKQKNFHQTKKMP*LWSNQK*FH*QRTKKKKK VKKLQARTHLGCLGKVKAPVT*KILQVLMLK*I VRLTR*MTQQRVNKKIN**QGHKMKRRNKEMEL NDNPQHRKASPGSGGAREDSSTRCGGGWGWGEGK SHGRTGNPLL*FLQLHFVPFICRKRKKKKKKKV SFNLVEGPMLTHLSGIILVISVFSLTTLPQGHS GLIEHSHVYPGPCLLSWLFHKAGHTVVYSKEGEE DSGLISCRINFRNQ*AQQQYNPQTEEAG*KMGK SLGNHLEKVWTMR*KYFLQGSQGTICSKWLMRN MEAEFFPEQSAEA*QKGGPWQPSGSLPS*PWWQV HPSLTLTFSRAYLVHEVFTWRLPARGDSLLHNC KGWPFARCYS*KLRKGAVHLGPKHMPMNIKSSS *NCCEFSIKEYPVFTQGLTAHRVVSFEITGN*SF CLQFCLPGLCLNAVTCLCQVQG*FNGWSPHPEN PEGEDTALKGGSSTKNPRCQWYSGHKGWRP*GC QASSPISILEPV*GQMPTSHSLPGFQSHVIVFSG NISFVAYLLKSIFLVRALELKLTYIVFP*QREK NSGLLFLK*KDVLLSKYPIKIFSFALF*WKIHL IFL*LWF*PF*ICYPFWMAYLIYWNS*QAILQHM HYILTNFF**NQDISKNDIYVTS*CGIMAFCCY SSLIWKHLYPLLPLDVVAFQKSNEKAHFKESKK RCHPNIDSFYISCHLYNSIESCRHSCEVFDKCNL LEARFSCDSQMLVLLSLSNELQLKKT*GKQRDR DFLMNNDGRDLMSLLETARMEIIQPWHSPYHQ* QSFYSFISNVGGLEVEGGNSLCLKILEE*K*SLY PGN*ENEE*ISSARLLNLFILPSIIRLCFHFCF VMLFGCYLAIQFYALKQMIKLISNSIVKY*QSN YSQIKDT*TQKTFICWCCHCTGCTMK*I*KAD*F SRT*ILDVNFQPNSNTAMWHEELQEGGRKIALC *SCLHTEDQSRPSP**F*LKWSSL*ERQRGFSS SW*LFR*WTSLLS*KITKKASESLSVILGHILIT AANCKSFYETLKSLFVN*YSSA*CT*FEKTFLC MWRMSALL*KITKLAEYVQYIFLGNRVFIT*FI KDLPYPDICDIKENQKKSNFLDQDMFLLNANKCS LLLARKKKWLLISGINC*IG*YVPLLLNRHLNA GLLLNLFVSFHLCHTLKNNCCLHTIFVRAEYK* NLFERIM*

Frame 2

YREADDRLRPYQGCTCHILERLHKKPLKIGALW ASGIQRAHFLETQHSGRSHRASLCRCHPYCRAN SQGPY*WFPQGRPCKLL*IISEARERK*HPISC ISLHLNRHLWLSQRACCSHCPQHH*GMACQESP* GGSDHFLCLLRS*LQNLQKENE*VFLRRR***R RRGC*NERRFR*ERSRGEAKCGRNGRAEPRCRW CQHCHCARPCFRRGS*RL*R*RFCKG*KYYKRR* SDRSFCA*PRSSRWTRE*FNEE*NKN*NRIAEL IYGNRRTFIKPRRCRDCGATRSDSINRGPRRKR R*KSSRRGHT*DAWEK*RLQ*PRKYSRS*C*NE* SG*QGK*PNRESTRRSTNSRGTR*SEGTKKWN* MTILSIARPLLALGELGKIAAHAVEEGGGGGKA SPMEGRGILYSNFSSCILFRLSAEKERKKRKKKF PLIWWRDPC*RIFQALSL*FLSFLLQLCPRVTV A*LNTHMCILAPVCFLGYFTKLVTQWFIQRKGR KTVV**AAG*ILGINEPSSSIIPRQRRQDRKWAK ASETTWKRSGQ*GENIFFRVPKAQFVPSG**EI WKLNFFQSRVQRHNRRVGPGSHLGLFLPNHGGR CILL*H*LSAELTWFMRSSHGDYQQEVTLCCITV KDGPLLGVTVKS*EKGQCI*DPNICL*ISKAPP EIAVSFP*KNILSSPKA*QPTEWSHLKLQEIRA FACSSAFLACV*MLSLVYAKFKADSMVGPLTQKT LKGRIQL*RGAAVLKTQDASGIVGTRDGDHEDA RHHHQYLS*SQYKARCLLPTASPGSKVMSLFSV ETYRLLHIFLNPSSL*GL*N*NLLILFFLNRGRK IVDYYF*NKRMFCFLNIPSKSSVLHFFDGKFIL SSYDFGFSLSEFVTPSGWLI*YTGIVNKLYFSI CTIF*QIFFNKIKTSARMTFT*PHNVGLWPSVAI PV*YGSIYILYCR*MLLLFRKVTKRLILKNPRN DVIQILTVSTFHAIFITQLKVAVILVKYLTSAI C*KLVFLVTPKC*CYLASVMSYS*KKHEGNRGTE IF**TMMEET*CPC*KQPGWKLSSPGILLIIND SHFIHLFQMWVG*KWKEGILSA*KF*KNESNLC IQETKRMRNKYLQPDS*ICLFFHL*LDCVFIFAL SCFLVVIWLYSFML*NK**S*FPIQ**NINNLT IARSKTPEHRKPLFAGAAIAQAVQ*NRFEKLID FPAHKFWMSISNQTPIQLCGMKSYRREGGK*PYV SHVCIQRIKVGLHHNSSN*NGPRCRRDKGAFPL AGNYSDDGQVFFHKRLQRRHPNHCL*YWVTY*S LQLIVNLSMKH*KASL*INTVLLDALDLKRHFSV CGACRLCFEK*QS*QNMFNIFSWGIGFLLHDSL RICLTLTFVI*RKIRKKVIFLIKICFYLMQINV VCCLQGKKNGF*YLV*TAK*DNTCLFC*TGI*ML DCF*ICLFLFICAIH*KTTVAFILYLLEQNTNK ICLRG*CE